Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053471.t1 | PNX66407.1 | 55.8 | 95 | 41 | 1 | 19 | 113 | 5 | 98 | 7.10E-23 | 116.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053471.t1 | A0A2K3KJF0 | 55.8 | 95 | 41 | 1 | 19 | 113 | 5 | 98 | 5.1e-23 | 116.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene053471.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTTGTGGAGAGGTGTTTA+AGG | 0.215079 | 4.3:+26304411 | MS.gene053471:CDS |
| TGTTGTTAGCGAAGATAATA+TGG | 0.354164 | 4.3:+26304225 | MS.gene053471:CDS |
| GTTCTTGCCCTCTTATTAGT+AGG | 0.385967 | 4.3:-26304313 | None:intergenic |
| TGTGGAGAGGTGTTTAAGGC+AGG | 0.423447 | 4.3:+26304415 | MS.gene053471:CDS |
| ATGATTCACCTACTAATAAG+AGG | 0.444593 | 4.3:+26304305 | MS.gene053471:CDS |
| AAAATAGGGCTCGGTGATGA+TGG | 0.458382 | 4.3:+26304367 | MS.gene053471:CDS |
| AACTGTCCAGGGTGATGTCC+AGG | 0.490878 | 4.3:+26304270 | MS.gene053471:CDS |
| AAAATTAAATATGCTGATGT+TGG | 0.512010 | 4.3:+26304490 | MS.gene053471:CDS |
| AGGGAAAAGTGTAATGGTTG+TGG | 0.529106 | 4.3:+26304397 | MS.gene053471:CDS |
| GGGTGAGAATGAAACTGTCC+AGG | 0.534133 | 4.3:+26304258 | MS.gene053471:CDS |
| GAACTTGCAAGGCTGAATGT+TGG | 0.549103 | 4.3:+26304332 | MS.gene053471:CDS |
| ATTCCAAGGGAAAAGTGTAA+TGG | 0.554789 | 4.3:+26304391 | MS.gene053471:CDS |
| GGAGAGGTGTTTAAGGCAGG+AGG | 0.555309 | 4.3:+26304418 | MS.gene053471:CDS |
| AAGGCAGGAGGTAAAGACTA+CGG | 0.573526 | 4.3:+26304430 | MS.gene053471:CDS |
| AGGCAGGAGGTAAAGACTAC+GGG | 0.586696 | 4.3:+26304431 | MS.gene053471:CDS |
| CGAAGATAATATGGATACTG+TGG | 0.589581 | 4.3:+26304234 | MS.gene053471:CDS |
| AGATAATATGGATACTGTGG+AGG | 0.601464 | 4.3:+26304237 | MS.gene053471:CDS |
| TAAGAGGGCAAGAACTTGCA+AGG | 0.602287 | 4.3:+26304321 | MS.gene053471:CDS |
| CTAACAACATCATTATCGAA+TGG | 0.612214 | 4.3:-26304211 | None:intergenic |
| GATAATATGGATACTGTGGA+GGG | 0.631737 | 4.3:+26304238 | MS.gene053471:CDS |
| CGGTGATGATGGAATTCCAA+GGG | 0.635650 | 4.3:+26304378 | MS.gene053471:CDS |
| TGATTCACCTACTAATAAGA+GGG | 0.650107 | 4.3:+26304306 | MS.gene053471:CDS |
| TCGGTGATGATGGAATTCCA+AGG | 0.661836 | 4.3:+26304377 | MS.gene053471:CDS |
| GGTGAGAATGAAACTGTCCA+GGG | 0.665787 | 4.3:+26304259 | MS.gene053471:CDS |
| CATCACCCTGGACATCACCC+TGG | 0.671830 | 4.3:-26304276 | None:intergenic |
| ACTGTCCAGGGTGATGTCCA+GGG | 0.682302 | 4.3:+26304271 | MS.gene053471:CDS |
| AATCATCATCAACATCACCC+TGG | 0.689396 | 4.3:-26304288 | None:intergenic |
| AAAGTGTAATGGTTGTGGAG+AGG | 0.717802 | 4.3:+26304402 | MS.gene053471:CDS |
| CAAGTGATGATTGACTTGCA+AGG | 0.750461 | 4.3:+26304514 | MS.gene053471:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATAAAATTTAGATTTGT+GGG | - | chr4.3:26304817-26304836 | None:intergenic | 10.0% |
| !! | CAATATAAAATTTAGATTTG+TGG | - | chr4.3:26304818-26304837 | None:intergenic | 15.0% |
| !!! | CACAAATCTAAATTTTATAT+TGG | + | chr4.3:26304816-26304835 | MS.gene053471:intron | 15.0% |
| !!! | TTTTTCGAAATCTAAAAAAT+TGG | - | chr4.3:26304951-26304970 | None:intergenic | 15.0% |
| !! | AAAATGTAGTCAAATTAATG+TGG | + | chr4.3:26304968-26304987 | MS.gene053471:intron | 20.0% |
| !! | AAAATTAAATATGCTGATGT+TGG | + | chr4.3:26304490-26304509 | MS.gene053471:CDS | 20.0% |
| !! | AAATACTTATTTAGAAAGCT+TGG | + | chr4.3:26305060-26305079 | MS.gene053471:intron | 20.0% |
| !! | AATCCTACTTATTCTAATAT+TGG | - | chr4.3:26304883-26304902 | None:intergenic | 20.0% |
| !! | GGAAAATGTTTACAAAAATA+GGG | + | chr4.3:26304353-26304372 | MS.gene053471:CDS | 20.0% |
| !! | GTAAAATATCTTTACACTAA+TGG | - | chr4.3:26305149-26305168 | None:intergenic | 20.0% |
| !! | TAACCAATATTAGAATAAGT+AGG | + | chr4.3:26304877-26304896 | MS.gene053471:intron | 20.0% |
| !! | TGGAAAATGTTTACAAAAAT+AGG | + | chr4.3:26304352-26304371 | MS.gene053471:CDS | 20.0% |
| !! | TGTAAAGTCATTTATTTGAT+TGG | + | chr4.3:26305261-26305280 | MS.gene053471:intron | 20.0% |
| !!! | GATAAACTTTAGAAATATGT+TGG | - | chr4.3:26305099-26305118 | None:intergenic | 20.0% |
| ! | ATAAATTGTCACAACAAAGT+TGG | + | chr4.3:26305030-26305049 | MS.gene053471:intron | 25.0% |
| !! | TAAGTCTTGACTATGTATTT+TGG | + | chr4.3:26304765-26304784 | MS.gene053471:intron | 25.0% |
| !!! | AGAGAGGTTCTATTTTTATT+AGG | + | chr4.3:26304670-26304689 | MS.gene053471:intron | 25.0% |
| AAAATTTAGATTTGTGGGAC+AGG | - | chr4.3:26304812-26304831 | None:intergenic | 30.0% | |
| AAATTAGAGAACAGCAAAAC+AGG | - | chr4.3:26304724-26304743 | None:intergenic | 30.0% | |
| ACGTTGTCAATTTATCATGA+TGG | + | chr4.3:26305167-26305186 | MS.gene053471:intron | 30.0% | |
| ATGATTCACCTACTAATAAG+AGG | + | chr4.3:26304305-26304324 | MS.gene053471:CDS | 30.0% | |
| ATGTTTACAAAAATAGGGCT+CGG | + | chr4.3:26304358-26304377 | MS.gene053471:CDS | 30.0% | |
| CTAACAACATCATTATCGAA+TGG | - | chr4.3:26304214-26304233 | None:intergenic | 30.0% | |
| CTTATTTAGAAAGCTTGGAT+TGG | + | chr4.3:26305065-26305084 | MS.gene053471:intron | 30.0% | |
| GTTCAATATCGTTAAGACTA+GGG | - | chr4.3:26304924-26304943 | None:intergenic | 30.0% | |
| TAACCAATGAGCAGATTATA+TGG | + | chr4.3:26304563-26304582 | MS.gene053471:intron | 30.0% | |
| TGATTCACCTACTAATAAGA+GGG | + | chr4.3:26304306-26304325 | MS.gene053471:CDS | 30.0% | |
| TGTTCAATATCGTTAAGACT+AGG | - | chr4.3:26304925-26304944 | None:intergenic | 30.0% | |
| TGTTGTTAGCGAAGATAATA+TGG | + | chr4.3:26304225-26304244 | MS.gene053471:CDS | 30.0% | |
| ! | ACACATATGTTGCTTAGATA+AGG | + | chr4.3:26304540-26304559 | MS.gene053471:intron | 30.0% |
| ! | ACTTTAGAAATATGTTGGAG+TGG | - | chr4.3:26305094-26305113 | None:intergenic | 30.0% |
| ! | CTTTAGAAATATGTTGGAGT+GGG | - | chr4.3:26305093-26305112 | None:intergenic | 30.0% |
| ! | TTTTCTTGCACTTCTTCATA+TGG | - | chr4.3:26304474-26304493 | None:intergenic | 30.0% |
| AGATAATATGGATACTGTGG+AGG | + | chr4.3:26304237-26304256 | MS.gene053471:CDS | 35.0% | |
| CGAAGATAATATGGATACTG+TGG | + | chr4.3:26304234-26304253 | MS.gene053471:CDS | 35.0% | |
| GATAATATGGATACTGTGGA+GGG | + | chr4.3:26304238-26304257 | MS.gene053471:CDS | 35.0% | |
| ! | ATTCCAAGGGAAAAGTGTAA+TGG | + | chr4.3:26304391-26304410 | MS.gene053471:CDS | 35.0% |
| !! | TTCTACTGATGTTGCTTTCA+CGG | + | chr4.3:26304599-26304618 | MS.gene053471:intron | 35.0% |
| AAAGTGTAATGGTTGTGGAG+AGG | + | chr4.3:26304402-26304421 | MS.gene053471:CDS | 40.0% | |
| AACAGAGGGATAAGTAACTC+CGG | - | chr4.3:26304639-26304658 | None:intergenic | 40.0% | |
| AATCATCATCAACATCACCC+TGG | - | chr4.3:26304291-26304310 | None:intergenic | 40.0% | |
| ATCAGTAGAAGCTCCAAGTT+TGG | - | chr4.3:26304589-26304608 | None:intergenic | 40.0% | |
| CGAAACAACAACACTACTTG+AGG | - | chr4.3:26305003-26305022 | None:intergenic | 40.0% | |
| GCAGATTATATGGCCAAACT+TGG | + | chr4.3:26304573-26304592 | MS.gene053471:intron | 40.0% | |
| GTTCTTGCCCTCTTATTAGT+AGG | - | chr4.3:26304316-26304335 | None:intergenic | 40.0% | |
| TCTCATCTGTTCTCAACAGA+GGG | - | chr4.3:26304653-26304672 | None:intergenic | 40.0% | |
| TGGCCATATAATCTGCTCAT+TGG | - | chr4.3:26304569-26304588 | None:intergenic | 40.0% | |
| ! | AGGGAAAAGTGTAATGGTTG+TGG | + | chr4.3:26304397-26304416 | MS.gene053471:CDS | 40.0% |
| ! | CAACCATTACACTTTTCCCT+TGG | - | chr4.3:26304397-26304416 | None:intergenic | 40.0% |
| !! | CAAGTGATGATTGACTTGCA+AGG | + | chr4.3:26304514-26304533 | MS.gene053471:CDS | 40.0% |
| AAGGCAGGAGGTAAAGACTA+CGG | + | chr4.3:26304430-26304449 | MS.gene053471:CDS | 45.0% | |
| CTCTCATCTGTTCTCAACAG+AGG | - | chr4.3:26304654-26304673 | None:intergenic | 45.0% | |
| CTGTTGAGAACAGATGAGAG+AGG | + | chr4.3:26304654-26304673 | MS.gene053471:intron | 45.0% | |
| GAACTTGCAAGGCTGAATGT+TGG | + | chr4.3:26304332-26304351 | MS.gene053471:CDS | 45.0% | |
| GGTGAGAATGAAACTGTCCA+GGG | + | chr4.3:26304259-26304278 | MS.gene053471:CDS | 45.0% | |
| TAAGAGGGCAAGAACTTGCA+AGG | + | chr4.3:26304321-26304340 | MS.gene053471:CDS | 45.0% | |
| !! | AAAATAGGGCTCGGTGATGA+TGG | + | chr4.3:26304367-26304386 | MS.gene053471:CDS | 45.0% |
| !! | CGGTGATGATGGAATTCCAA+GGG | + | chr4.3:26304378-26304397 | MS.gene053471:CDS | 45.0% |
| !! | TCGGTGATGATGGAATTCCA+AGG | + | chr4.3:26304377-26304396 | MS.gene053471:CDS | 45.0% |
| !! | TGGTTGTGGAGAGGTGTTTA+AGG | + | chr4.3:26304411-26304430 | MS.gene053471:CDS | 45.0% |
| AGAGGGATAAGTAACTCCGG+AGG | - | chr4.3:26304636-26304655 | None:intergenic | 50.0% | |
| AGGCAGGAGGTAAAGACTAC+GGG | + | chr4.3:26304431-26304450 | MS.gene053471:CDS | 50.0% | |
| GGGTGAGAATGAAACTGTCC+AGG | + | chr4.3:26304258-26304277 | MS.gene053471:CDS | 50.0% | |
| !! | TGTGGAGAGGTGTTTAAGGC+AGG | + | chr4.3:26304415-26304434 | MS.gene053471:CDS | 50.0% |
| AACTGTCCAGGGTGATGTCC+AGG | + | chr4.3:26304270-26304289 | MS.gene053471:CDS | 55.0% | |
| ACTGTCCAGGGTGATGTCCA+GGG | + | chr4.3:26304271-26304290 | MS.gene053471:CDS | 55.0% | |
| !! | GGAGAGGTGTTTAAGGCAGG+AGG | + | chr4.3:26304418-26304437 | MS.gene053471:CDS | 55.0% |
| CATCACCCTGGACATCACCC+TGG | - | chr4.3:26304279-26304298 | None:intergenic | 60.0% | |
| CACGGTTCACTCTGCGCCTC+CGG | + | chr4.3:26304617-26304636 | MS.gene053471:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 26304196 | 26305319 | 26304196 | ID=MS.gene053471 |
| chr4.3 | mRNA | 26304196 | 26305319 | 26304196 | ID=MS.gene053471.t1;Parent=MS.gene053471 |
| chr4.3 | exon | 26304196 | 26304535 | 26304196 | ID=MS.gene053471.t1.exon1;Parent=MS.gene053471.t1 |
| chr4.3 | CDS | 26304196 | 26304535 | 26304196 | ID=cds.MS.gene053471.t1;Parent=MS.gene053471.t1 |
| chr4.3 | exon | 26305315 | 26305319 | 26305315 | ID=MS.gene053471.t1.exon2;Parent=MS.gene053471.t1 |
| chr4.3 | CDS | 26305315 | 26305319 | 26305315 | ID=cds.MS.gene053471.t1;Parent=MS.gene053471.t1 |
| Gene Sequence |
| Protein sequence |
