AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053517

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053517.t1 MTR_4g085910 97.340 376 10 0 1 376 1 376 0.0 751
MS.gene053517.t1 MTR_3g101780 45.433 427 151 12 14 376 23 431 9.35e-91 280
MS.gene053517.t1 MTR_7g104480 34.307 411 176 16 24 370 45 425 7.22e-35 133
MS.gene053517.t1 MTR_8g043960 45.802 131 63 2 17 144 23 148 1.16e-29 116
MS.gene053517.t1 MTR_8g043960 75.000 56 13 1 296 350 237 292 2.77e-15 76.3
MS.gene053517.t1 MTR_7g088090 45.385 130 66 2 17 144 15 141 1.51e-26 108
MS.gene053517.t1 MTR_7g088090 54.348 92 34 2 288 375 232 319 1.51e-16 80.1
MS.gene053517.t1 MTR_3g010660 45.455 132 60 4 18 144 20 144 7.68e-25 103
MS.gene053517.t1 MTR_3g010660 68.519 54 16 1 296 349 246 298 1.62e-14 73.9
MS.gene053517.t1 MTR_6g026800 41.611 149 75 4 18 156 16 162 6.48e-24 100
MS.gene053517.t1 MTR_5g088230 42.188 128 59 4 18 138 17 136 4.12e-16 77.8
MS.gene053517.t1 MTR_5g088230 42.188 128 59 4 18 138 22 141 4.34e-16 77.8
MS.gene053517.t1 MTR_2g086340 36.296 135 68 4 19 142 23 150 2.16e-14 73.9
MS.gene053517.t1 MTR_2g086390 50.575 87 41 2 286 371 194 279 7.04e-13 68.9
MS.gene053517.t1 MTR_2g086390 60.000 60 23 1 286 344 194 253 9.58e-13 68.6
MS.gene053517.t1 MTR_1g098590 60.377 53 21 0 296 348 157 209 1.27e-12 67.4
MS.gene053517.t1 MTR_1g098590 60.377 53 21 0 296 348 157 209 1.48e-12 67.0
MS.gene053517.t1 MTR_7g015990 68.627 51 13 1 301 348 120 170 2.01e-12 65.9
MS.gene053517.t1 MTR_1g098590 60.377 53 21 0 296 348 157 209 2.50e-12 66.6
MS.gene053517.t1 MTR_5g022780 51.220 82 23 2 301 365 166 247 8.01e-12 65.1
MS.gene053517.t1 MTR_4g094390 60.377 53 21 0 296 348 125 177 1.62e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053517.t1 AT1G49720 45.897 390 160 11 18 376 23 392 2.69e-92 282
MS.gene053517.t1 AT1G49720 45.897 390 160 11 18 376 23 392 2.69e-92 282
MS.gene053517.t1 AT4G34000 44.570 442 154 17 15 376 24 454 1.02e-91 283
MS.gene053517.t1 AT4G34000 44.570 442 154 17 15 376 24 454 1.02e-91 283
MS.gene053517.t1 AT4G34000 44.570 442 154 17 15 376 24 454 1.02e-91 283
MS.gene053517.t1 AT1G45249 46.973 413 148 16 18 376 21 416 1.29e-88 273
MS.gene053517.t1 AT1G45249 46.973 413 148 16 18 376 36 431 1.85e-88 273
MS.gene053517.t1 AT1G45249 46.973 413 148 16 18 376 36 431 1.85e-88 273
MS.gene053517.t1 AT1G49720 46.301 365 150 10 18 356 23 367 4.17e-88 272
MS.gene053517.t1 AT3G19290 44.104 424 164 13 10 376 25 432 1.58e-87 271
MS.gene053517.t1 AT3G19290 44.313 422 165 13 10 376 25 431 2.31e-87 271
MS.gene053517.t1 AT3G19290 44.313 422 165 13 10 376 25 431 2.31e-87 271
MS.gene053517.t1 AT1G45249 45.755 424 148 17 18 376 21 427 6.23e-85 264
MS.gene053517.t1 AT4G34000 44.717 407 140 16 15 347 24 419 1.39e-84 264
MS.gene053517.t1 AT3G19290 43.719 398 158 11 10 356 25 407 2.68e-82 257
MS.gene053517.t1 AT1G45249 43.889 360 135 15 18 327 21 363 2.50e-64 209
MS.gene053517.t1 AT1G45249 43.889 360 135 15 18 327 21 363 2.50e-64 209
MS.gene053517.t1 AT3G19290 41.530 366 148 11 10 324 25 375 8.14e-64 209
MS.gene053517.t1 AT1G45249 42.744 379 143 17 18 339 21 382 4.35e-63 207
MS.gene053517.t1 AT1G45249 42.744 379 143 17 18 339 21 382 4.35e-63 207
MS.gene053517.t1 AT1G45249 42.744 379 143 17 18 339 21 382 4.35e-63 207
MS.gene053517.t1 AT1G45249 42.744 379 143 17 18 339 21 382 4.35e-63 207
MS.gene053517.t1 AT5G42910 36.022 372 167 15 13 353 15 346 4.98e-39 143
MS.gene053517.t1 AT3G56850 49.306 144 57 5 10 145 8 143 4.37e-31 120
MS.gene053517.t1 AT3G56850 58.242 91 30 3 290 376 211 297 6.42e-17 80.9
MS.gene053517.t1 AT2G41070 35.503 169 83 2 10 173 8 155 8.32e-26 104
MS.gene053517.t1 AT2G41070 65.672 67 20 1 290 353 177 243 1.77e-16 78.6
MS.gene053517.t1 AT2G41070 35.503 169 83 2 10 173 8 155 1.18e-25 104
MS.gene053517.t1 AT2G41070 68.182 66 20 1 280 344 169 234 4.99e-16 77.8
MS.gene053517.t1 AT2G41070 35.503 169 83 2 10 173 8 155 1.18e-25 104
MS.gene053517.t1 AT2G41070 68.182 66 20 1 280 344 169 234 4.99e-16 77.8
MS.gene053517.t1 AT2G41070 35.503 169 83 2 10 173 8 155 1.18e-25 104
MS.gene053517.t1 AT2G41070 68.182 66 20 1 280 344 169 234 4.99e-16 77.8
MS.gene053517.t1 AT2G41070 35.503 169 83 2 10 173 17 164 1.44e-25 104
MS.gene053517.t1 AT2G41070 68.182 66 20 1 280 344 178 243 4.85e-16 77.8
MS.gene053517.t1 AT3G44460 44.118 136 54 6 18 140 35 161 2.54e-20 91.3
MS.gene053517.t1 AT2G36270 35.676 185 59 3 16 140 35 219 8.93e-20 90.9
MS.gene053517.t1 AT2G36270 67.606 71 20 1 289 356 341 411 5.53e-18 85.5
MS.gene053517.t1 AT2G36270 35.676 185 59 3 16 140 35 219 8.93e-20 90.9
MS.gene053517.t1 AT2G36270 67.606 71 20 1 289 356 341 411 5.53e-18 85.5
MS.gene053517.t1 AT2G36270 35.870 184 58 3 17 140 36 219 1.23e-19 90.5
MS.gene053517.t1 AT2G36270 63.636 77 22 2 289 362 341 414 4.63e-18 85.9
MS.gene053517.t1 AT4G35900 52.564 78 26 2 302 376 216 285 1.58e-13 70.9
MS.gene053517.t1 AT2G17770 52.632 76 31 2 301 376 125 195 3.92e-13 68.2
MS.gene053517.t1 AT5G44080 63.793 58 20 1 289 345 219 276 1.15e-12 68.6
MS.gene053517.t1 AT1G03970 60.656 61 23 1 289 348 175 235 2.85e-12 67.0

Find 94 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATACAGGCAGAGTTTA+TGG 0.190438 4.3:+26890709 MS.gene053517:intron
ATGCTACAGTTGTAGGTTTC+GGG 0.224383 4.3:-26889976 None:intergenic
CTAAACCATTCATTATTATT+TGG 0.243582 4.3:-26889791 None:intergenic
CAACAACCAAATGGAAATAA+TGG 0.257332 4.3:+26889857 MS.gene053517:CDS
AAAGAAAAGTTGATGAAGTT+TGG 0.260665 4.3:+26889630 MS.gene053517:CDS
CTACCACCATTTGGGTTGTT+AGG 0.265606 4.3:-26889563 None:intergenic
CCTAATGTTGGTTGTCTTTG+AGG 0.269893 4.3:-26889686 None:intergenic
GAAGAAAATGCTACAGTTGT+AGG 0.325476 4.3:-26889983 None:intergenic
TACTTGAGAAGGACACTTAC+AGG 0.325650 4.3:+26890779 MS.gene053517:CDS
CTTGCTAGATTAACACTTCC+AGG 0.329485 4.3:-26889374 None:intergenic
AATGCTACAGTTGTAGGTTT+CGG 0.329976 4.3:-26889977 None:intergenic
TTACTTCCTGGAGAAGTAAC+AGG 0.334643 4.3:-26890106 None:intergenic
GGAGGAAGAGGAACATGTTT+AGG 0.346580 4.3:-26889908 None:intergenic
AACAACCAAATGGAAATAAT+GGG 0.348398 4.3:+26889858 MS.gene053517:CDS
TCAATTTGTTGAGCATGATT+AGG 0.349738 4.3:-26889764 None:intergenic
TTTGCAAACTACCACCATTT+GGG 0.380553 4.3:-26889571 None:intergenic
AAGTGTGTTCCTGTAGAAAA+AGG 0.390691 4.3:+26890211 MS.gene053517:CDS
CTCCCTCTATTGATTGCATA+AGG 0.393176 4.3:-26890187 None:intergenic
GAGGTCACGGGCTCGCAAAC+AGG 0.396241 4.3:+26890282 MS.gene053517:CDS
ATATCCCCATTATTTCCATT+TGG 0.401525 4.3:-26889863 None:intergenic
TATGGAAATTCAGAAAAGTA+AGG 0.412322 4.3:+26890727 MS.gene053517:CDS
ATGAGTTACAAAGCACCATT+GGG 0.416914 4.3:+26889426 MS.gene053517:CDS
AATGGCAGAAGCGTAGTTCC+TGG 0.429990 4.3:+26890043 MS.gene053517:CDS
AACATTAGGTGAAGTGACTT+TGG 0.430676 4.3:+26889700 MS.gene053517:CDS
CCTCAAAGACAACCAACATT+AGG 0.434960 4.3:+26889686 MS.gene053517:CDS
GTTCCTTATGCAATCAATAG+AGG 0.435474 4.3:+26890184 MS.gene053517:CDS
CTATTGATTGCATAAGGAAC+TGG 0.435765 4.3:-26890181 None:intergenic
TTTCTTTGACTAAGTGTCCT+TGG 0.439082 4.3:-26889614 None:intergenic
CTTGGCTTTCAACAACCAAA+TGG 0.439959 4.3:+26889848 MS.gene053517:CDS
GGCTGAAGTTGCAAAACTTA+AGG 0.442265 4.3:+26890566 MS.gene053517:CDS
CGAGGAATTACAAAGAAAAC+AGG 0.444987 4.3:+26890596 MS.gene053517:CDS
CAAGCATTGACATCTCTAAC+TGG 0.446103 4.3:+26889521 MS.gene053517:CDS
GCATTGTTTAGCAAATGAAT+AGG 0.446181 4.3:-26890007 None:intergenic
TGATTCAAGTTAATTGATGA+AGG 0.454755 4.3:-26889929 None:intergenic
GATGAGTTACAAAGCACCAT+TGG 0.454822 4.3:+26889425 MS.gene053517:CDS
AAAGGCCTACACTGTTGAAC+TGG 0.460693 4.3:+26890542 MS.gene053517:intron
TTACGCTTTGTAATCAAATC+CGG 0.464033 4.3:-26890136 None:intergenic
AAAGACCAAATAATAATGAA+TGG 0.465002 4.3:+26889786 MS.gene053517:CDS
AACAATAACACCAATCTACT+TGG 0.466588 4.3:+26889830 MS.gene053517:CDS
GGAACTGGTGACAGTAAAGT+AGG 0.483859 4.3:-26890166 None:intergenic
AGGGATTTGATGAAAGATAG+TGG 0.487452 4.3:+26889653 MS.gene053517:CDS
ACAGCTCCTGTTACTTCTCC+AGG 0.488845 4.3:+26890100 MS.gene053517:CDS
GAGAATCAGCTGCGAGGTCA+CGG 0.489882 4.3:+26890269 MS.gene053517:CDS
TTGCTAAACAATGCTCAACT+TGG 0.492309 4.3:+26890016 MS.gene053517:CDS
GGGTCAAAATGAACTTCGCT+TGG 0.497946 4.3:+26889330 None:intergenic
TTAATTGATGAAGGAGGAAG+AGG 0.498832 4.3:-26889920 None:intergenic
AGTTAGAGATGTCAATGCTT+GGG 0.512680 4.3:-26889519 None:intergenic
AAAAGTTGATGAAGTTTGGA+GGG 0.514926 4.3:+26889634 MS.gene053517:CDS
TCTCTAACTGGTGGAGGAGT+AGG 0.517394 4.3:+26889533 MS.gene053517:CDS
GTTCCTGGTGTTGGAATGCA+TGG 0.529365 4.3:+26890058 MS.gene053517:CDS
GAAAAGTTGATGAAGTTTGG+AGG 0.529458 4.3:+26889633 MS.gene053517:CDS
CAGTTAGAGATGTCAATGCT+TGG 0.539167 4.3:-26889520 None:intergenic
GAAGGACCTAACAACCCAAA+TGG 0.541425 4.3:+26889557 MS.gene053517:CDS
GTTGTAGGTTTCGGGAAAAG+TGG 0.542287 4.3:-26889968 None:intergenic
AACAGGAGCTGTGACATTTG+AGG 0.542288 4.3:-26890089 None:intergenic
AGAATCAGCTGCGAGGTCAC+GGG 0.543539 4.3:+26890270 MS.gene053517:CDS
ATAAAATAAAATACTTGAGA+AGG 0.546602 4.3:+26890768 MS.gene053517:CDS
TTGTTGAAAGCCAAGTAGAT+TGG 0.562943 4.3:-26889840 None:intergenic
TGAGGGAGTATATTGAAGAC+TGG 0.563542 4.3:-26889397 None:intergenic
GGTCAAAATGAACTTCGCTT+GGG 0.564260 4.3:+26889331 None:intergenic
AAAGTCACTTCACCTAATGT+TGG 0.567590 4.3:-26889698 None:intergenic
ATTAGGTGAAGTGACTTTGG+AGG 0.571566 4.3:+26889703 MS.gene053517:CDS
GAACTGGTGACAGTAAAGTA+GGG 0.576448 4.3:-26890165 None:intergenic
TCCAGGAAGTAAAATGTCGC+CGG 0.577416 4.3:+26890117 MS.gene053517:CDS
GCAATGAGTGTGAAGACACC+TGG 0.579770 4.3:+26889356 MS.gene053517:CDS
TTGACATCTCTAACTGGTGG+AGG 0.580857 4.3:+26889527 MS.gene053517:CDS
TTCAAGTTAATTGATGAAGG+AGG 0.584325 4.3:-26889926 None:intergenic
TCAGAAAAGTAAGGAGGACC+TGG 0.603007 4.3:+26890736 MS.gene053517:CDS
TTTGTAACTCATCAAAAGTG+AGG 0.603693 4.3:-26889415 None:intergenic
AGAAGCGTAGTTCCTGGTGT+TGG 0.604312 4.3:+26890049 MS.gene053517:CDS
TTGTAACTCATCAAAAGTGA+GGG 0.607665 4.3:-26889414 None:intergenic
TTCCTTATGCAATCAATAGA+GGG 0.608134 4.3:+26890185 MS.gene053517:CDS
GTAGGTTTCGGGAAAAGTGG+TGG 0.611415 4.3:-26889965 None:intergenic
GGTTTCGGGAAAAGTGGTGG+CGG 0.614066 4.3:-26889962 None:intergenic
AAGTGTCCTTGGCAATGTCA+AGG 0.621700 4.3:-26889603 None:intergenic
AATGCTCAACTTGGTAACAA+TGG 0.622719 4.3:+26890025 MS.gene053517:CDS
GAAGGACACTTACAGGTCCA+TGG 0.625288 4.3:+26890786 MS.gene053517:CDS
ACAACTCACATAATCTACCA+TGG 0.625778 4.3:-26890803 None:intergenic
GGCCTACACTGTTGAACTGG+AGG 0.626943 4.3:+26890545 MS.gene053517:intron
GCATTGACATCTCTAACTGG+TGG 0.627413 4.3:+26889524 MS.gene053517:CDS
ACTGGTGGAGGAGTAGGAGA+AGG 0.628322 4.3:+26889539 MS.gene053517:CDS
TTAACTTGAATCACTCTCAA+CGG 0.629011 4.3:+26889939 MS.gene053517:CDS
AAAGTAAGGAGGACCTGGTG+CGG 0.645011 4.3:+26890741 MS.gene053517:CDS
GGAAATTCAGAAAAGTAAGG+AGG 0.649721 4.3:+26890730 MS.gene053517:CDS
ACAACCAAATGGAAATAATG+GGG 0.659967 4.3:+26889859 MS.gene053517:CDS
AAACCATGCATTCCAACACC+AGG 0.661096 4.3:-26890061 None:intergenic
AAGGCTCCTTGACATTGCCA+AGG 0.666283 4.3:+26889597 MS.gene053517:CDS
GGACCTAACAACCCAAATGG+TGG 0.667509 4.3:+26889560 MS.gene053517:CDS
AGCCTCCAGTTCAACAGTGT+AGG 0.672463 4.3:-26890547 None:intergenic
CTTATGCAATCAATAGAGGG+AGG 0.679251 4.3:+26890188 MS.gene053517:CDS
TGAGTTACAAAGCACCATTG+GGG 0.703369 4.3:+26889427 MS.gene053517:CDS
GAGTTACAAAGCACCATTGG+GGG 0.704982 4.3:+26889428 MS.gene053517:CDS
AGAATAGAGAATCAGCTGCG+AGG 0.715608 4.3:+26890263 MS.gene053517:CDS
AAAACTTAAGGAAGTAAACG+AGG 0.734887 4.3:+26890578 MS.gene053517:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAATACTTGAGA+AGG + chr4.3:26890768-26890787 MS.gene053517:CDS 15.0%
!!! AAATATTTGTTTCTTTTGAA+TGG + chr4.3:26890519-26890538 MS.gene053517:intron 15.0%
!! AAAGACCAAATAATAATGAA+TGG + chr4.3:26889786-26889805 MS.gene053517:CDS 20.0%
!! ATACATTATATGTGTATTGA+AGG - chr4.3:26890421-26890440 None:intergenic 20.0%
!! CTAAACCATTCATTATTATT+TGG - chr4.3:26889794-26889813 None:intergenic 20.0%
!! TATTAAAATGATGTAAGCAT+TGG + chr4.3:26890475-26890494 MS.gene053517:intron 20.0%
! AACAACCAAATGGAAATAAT+GGG + chr4.3:26889858-26889877 MS.gene053517:CDS 25.0%
! ATAACAAGATCATAAGCTTA+AGG + chr4.3:26890439-26890458 MS.gene053517:intron 25.0%
! ATGAACTCTTGAAAAACATA+TGG + chr4.3:26889483-26889502 MS.gene053517:CDS 25.0%
! TATGGAAATTCAGAAAAGTA+AGG + chr4.3:26890727-26890746 MS.gene053517:CDS 25.0%
!! TGATTCAAGTTAATTGATGA+AGG - chr4.3:26889932-26889951 None:intergenic 25.0%
!!! AAAGAAAAGTTGATGAAGTT+TGG + chr4.3:26889630-26889649 MS.gene053517:CDS 25.0%
!!! TTTGTTTCTTTTGAATGGAA+AGG + chr4.3:26890524-26890543 MS.gene053517:intron 25.0%
AAAACTTAAGGAAGTAAACG+AGG + chr4.3:26890578-26890597 MS.gene053517:CDS 30.0%
AACAATAACACCAATCTACT+TGG + chr4.3:26889830-26889849 MS.gene053517:CDS 30.0%
ACAACCAAATGGAAATAATG+GGG + chr4.3:26889859-26889878 MS.gene053517:CDS 30.0%
ATATCCCCATTATTTCCATT+TGG - chr4.3:26889866-26889885 None:intergenic 30.0%
CAACAACCAAATGGAAATAA+TGG + chr4.3:26889857-26889876 MS.gene053517:CDS 30.0%
CATCAGTAATATGTTCACTA+AGG + chr4.3:26890650-26890669 MS.gene053517:intron 30.0%
GCATTGTTTAGCAAATGAAT+AGG - chr4.3:26890010-26890029 None:intergenic 30.0%
TTACAGTTTCTGACTACATT+TGG + chr4.3:26890686-26890705 MS.gene053517:intron 30.0%
TTACGCTTTGTAATCAAATC+CGG - chr4.3:26890139-26890158 None:intergenic 30.0%
TTCCTTATGCAATCAATAGA+GGG + chr4.3:26890185-26890204 MS.gene053517:CDS 30.0%
TTGTAACTCATCAAAAGTGA+GGG - chr4.3:26889417-26889436 None:intergenic 30.0%
TTTGTAACTCATCAAAAGTG+AGG - chr4.3:26889418-26889437 None:intergenic 30.0%
! AGATTTTGGATCAATGAACA+TGG + chr4.3:26889460-26889479 MS.gene053517:CDS 30.0%
! TTAACTTGAATCACTCTCAA+CGG + chr4.3:26889939-26889958 MS.gene053517:CDS 30.0%
! TTCAAGTTAATTGATGAAGG+AGG - chr4.3:26889929-26889948 None:intergenic 30.0%
!! AAAAGTTGATGAAGTTTGGA+GGG + chr4.3:26889634-26889653 MS.gene053517:CDS 30.0%
!! TCAATTTGTTGAGCATGATT+AGG - chr4.3:26889767-26889786 None:intergenic 30.0%
AAAGTCACTTCACCTAATGT+TGG - chr4.3:26889701-26889720 None:intergenic 35.0%
AACATTAGGTGAAGTGACTT+TGG + chr4.3:26889700-26889719 MS.gene053517:CDS 35.0%
AAGTGTGTTCCTGTAGAAAA+AGG + chr4.3:26890211-26890230 MS.gene053517:CDS 35.0%
AATGCTACAGTTGTAGGTTT+CGG - chr4.3:26889980-26889999 None:intergenic 35.0%
AGGGATTTGATGAAAGATAG+TGG + chr4.3:26889653-26889672 MS.gene053517:CDS 35.0%
AGTTAGAGATGTCAATGCTT+GGG - chr4.3:26889522-26889541 None:intergenic 35.0%
CGAGGAATTACAAAGAAAAC+AGG + chr4.3:26890596-26890615 MS.gene053517:CDS 35.0%
GAAGAAAATGCTACAGTTGT+AGG - chr4.3:26889986-26890005 None:intergenic 35.0%
GACTACATTTGGTATGATAC+AGG + chr4.3:26890697-26890716 MS.gene053517:intron 35.0%
GATCAGAGAAACAAAACAGA+TGG - chr4.3:26890362-26890381 None:intergenic 35.0%
GGAAATTCAGAAAAGTAAGG+AGG + chr4.3:26890730-26890749 MS.gene053517:CDS 35.0%
GTTCCTTATGCAATCAATAG+AGG + chr4.3:26890184-26890203 MS.gene053517:CDS 35.0%
TATGATACAGGCAGAGTTTA+TGG + chr4.3:26890709-26890728 MS.gene053517:intron 35.0%
TTAATTGATGAAGGAGGAAG+AGG - chr4.3:26889923-26889942 None:intergenic 35.0%
TTGCTAAACAATGCTCAACT+TGG + chr4.3:26890016-26890035 MS.gene053517:CDS 35.0%
TTGTTGAAAGCCAAGTAGAT+TGG - chr4.3:26889843-26889862 None:intergenic 35.0%
TTTCTTTGACTAAGTGTCCT+TGG - chr4.3:26889617-26889636 None:intergenic 35.0%
TTTGCAAACTACCACCATTT+GGG - chr4.3:26889574-26889593 None:intergenic 35.0%
! AATGCTCAACTTGGTAACAA+TGG + chr4.3:26890025-26890044 MS.gene053517:CDS 35.0%
! ATGAGTTACAAAGCACCATT+GGG + chr4.3:26889426-26889445 MS.gene053517:CDS 35.0%
! CTATTGATTGCATAAGGAAC+TGG - chr4.3:26890184-26890203 None:intergenic 35.0%
! CTTTCAACACCTTTTTCTAC+AGG - chr4.3:26890223-26890242 None:intergenic 35.0%
! TTTTGCAAACTACCACCATT+TGG - chr4.3:26889575-26889594 None:intergenic 35.0%
!! CTGTAGAAAAAGGTGTTGAA+AGG + chr4.3:26890221-26890240 MS.gene053517:CDS 35.0%
!! GAAAAGTTGATGAAGTTTGG+AGG + chr4.3:26889633-26889652 MS.gene053517:CDS 35.0%
!! TTATTTTATTTGTCCGCACC+AGG - chr4.3:26890757-26890776 None:intergenic 35.0%
ATGCTACAGTTGTAGGTTTC+GGG - chr4.3:26889979-26889998 None:intergenic 40.0%
ATTAGGTGAAGTGACTTTGG+AGG + chr4.3:26889703-26889722 MS.gene053517:CDS 40.0%
CAGTTAGAGATGTCAATGCT+TGG - chr4.3:26889523-26889542 None:intergenic 40.0%
CCTCAAAGACAACCAACATT+AGG + chr4.3:26889686-26889705 MS.gene053517:CDS 40.0%
CTTATGCAATCAATAGAGGG+AGG + chr4.3:26890188-26890207 MS.gene053517:CDS 40.0%
CTTGCTAGATTAACACTTCC+AGG - chr4.3:26889377-26889396 None:intergenic 40.0%
GAACTGGTGACAGTAAAGTA+GGG - chr4.3:26890168-26890187 None:intergenic 40.0%
GGCTGAAGTTGCAAAACTTA+AGG + chr4.3:26890566-26890585 MS.gene053517:CDS 40.0%
TACTTGAGAAGGACACTTAC+AGG + chr4.3:26890779-26890798 MS.gene053517:CDS 40.0%
TTACTTCCTGGAGAAGTAAC+AGG - chr4.3:26890109-26890128 None:intergenic 40.0%
! CCTAATGTTGGTTGTCTTTG+AGG - chr4.3:26889689-26889708 None:intergenic 40.0%
! CTCCCTCTATTGATTGCATA+AGG - chr4.3:26890190-26890209 None:intergenic 40.0%
! CTTGGCTTTCAACAACCAAA+TGG + chr4.3:26889848-26889867 MS.gene053517:CDS 40.0%
! GATGAGTTACAAAGCACCAT+TGG + chr4.3:26889425-26889444 MS.gene053517:CDS 40.0%
! GGTGGTAGTTTGCAAAAACA+AGG + chr4.3:26889578-26889597 MS.gene053517:CDS 40.0%
! TGAGGGAGTATATTGAAGAC+TGG - chr4.3:26889400-26889419 None:intergenic 40.0%
! TGAGTTACAAAGCACCATTG+GGG + chr4.3:26889427-26889446 MS.gene053517:CDS 40.0%
!! AGTGACTTTGGAGGAGTTTT+TGG + chr4.3:26889712-26889731 MS.gene053517:CDS 40.0%
!! CAAGCATTGACATCTCTAAC+TGG + chr4.3:26889521-26889540 MS.gene053517:CDS 40.0%
AAACCATGCATTCCAACACC+AGG - chr4.3:26890064-26890083 None:intergenic 45.0%
AAAGGCCTACACTGTTGAAC+TGG + chr4.3:26890542-26890561 MS.gene053517:intron 45.0%
AACAGGAGCTGTGACATTTG+AGG - chr4.3:26890092-26890111 None:intergenic 45.0%
AAGTGTCCTTGGCAATGTCA+AGG - chr4.3:26889606-26889625 None:intergenic 45.0%
AGAATAGAGAATCAGCTGCG+AGG + chr4.3:26890263-26890282 MS.gene053517:CDS 45.0%
GAAGGACCTAACAACCCAAA+TGG + chr4.3:26889557-26889576 MS.gene053517:CDS 45.0%
GGAACTGGTGACAGTAAAGT+AGG - chr4.3:26890169-26890188 None:intergenic 45.0%
GGAGGAAGAGGAACATGTTT+AGG - chr4.3:26889911-26889930 None:intergenic 45.0%
GTTGTAGGTTTCGGGAAAAG+TGG - chr4.3:26889971-26889990 None:intergenic 45.0%
TCAGAAAAGTAAGGAGGACC+TGG + chr4.3:26890736-26890755 MS.gene053517:CDS 45.0%
TCCAGGAAGTAAAATGTCGC+CGG + chr4.3:26890117-26890136 MS.gene053517:CDS 45.0%
TTGACATCTCTAACTGGTGG+AGG + chr4.3:26889527-26889546 MS.gene053517:CDS 45.0%
! ATCTTTTCCAACTCCCCCAA+TGG - chr4.3:26889444-26889463 None:intergenic 45.0%
! GAGTTACAAAGCACCATTGG+GGG + chr4.3:26889428-26889447 MS.gene053517:CDS 45.0%
! GCATTGACATCTCTAACTGG+TGG + chr4.3:26889524-26889543 MS.gene053517:CDS 45.0%
! GGGGGAGTTGGAAAAGATTT+TGG + chr4.3:26889446-26889465 MS.gene053517:CDS 45.0%
!! CTACCACCATTTGGGTTGTT+AGG - chr4.3:26889566-26889585 None:intergenic 45.0%
AAAGTAAGGAGGACCTGGTG+CGG + chr4.3:26890741-26890760 MS.gene053517:CDS 50.0%
AAGGCTCCTTGACATTGCCA+AGG + chr4.3:26889597-26889616 MS.gene053517:CDS 50.0%
AGCCTCCAGTTCAACAGTGT+AGG - chr4.3:26890550-26890569 None:intergenic 50.0%
GAAGGACACTTACAGGTCCA+TGG + chr4.3:26890786-26890805 MS.gene053517:CDS 50.0%
GCAATGAGTGTGAAGACACC+TGG + chr4.3:26889356-26889375 MS.gene053517:CDS 50.0%
GGACCTAACAACCCAAATGG+TGG + chr4.3:26889560-26889579 MS.gene053517:CDS 50.0%
GTAGGTTTCGGGAAAAGTGG+TGG - chr4.3:26889968-26889987 None:intergenic 50.0%
TCTCTAACTGGTGGAGGAGT+AGG + chr4.3:26889533-26889552 MS.gene053517:CDS 50.0%
! AATGGCAGAAGCGTAGTTCC+TGG + chr4.3:26890043-26890062 MS.gene053517:CDS 50.0%
! ACAGCTCCTGTTACTTCTCC+AGG + chr4.3:26890100-26890119 MS.gene053517:CDS 50.0%
! AGAAGCGTAGTTCCTGGTGT+TGG + chr4.3:26890049-26890068 MS.gene053517:CDS 50.0%
! GTTCCTGGTGTTGGAATGCA+TGG + chr4.3:26890058-26890077 MS.gene053517:CDS 50.0%
! TCCGGCGACATTTTACTTCC+TGG - chr4.3:26890121-26890140 None:intergenic 50.0%
!!! GAGGAGTTTTTGGTGAGAGC+TGG + chr4.3:26889722-26889741 MS.gene053517:CDS 50.0%
ACTGGTGGAGGAGTAGGAGA+AGG + chr4.3:26889539-26889558 MS.gene053517:CDS 55.0%
AGAATCAGCTGCGAGGTCAC+GGG + chr4.3:26890270-26890289 MS.gene053517:CDS 55.0%
GAGAATCAGCTGCGAGGTCA+CGG + chr4.3:26890269-26890288 MS.gene053517:CDS 55.0%
GGCCTACACTGTTGAACTGG+AGG + chr4.3:26890545-26890564 MS.gene053517:intron 55.0%
GGTTTCGGGAAAAGTGGTGG+CGG - chr4.3:26889965-26889984 None:intergenic 55.0%
! CAAAGCACCATTGGGGGAGT+TGG + chr4.3:26889434-26889453 MS.gene053517:CDS 55.0%
GAGGTCACGGGCTCGCAAAC+AGG + chr4.3:26890282-26890301 MS.gene053517:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 26889338 26890811 26889338 ID=MS.gene053517
chr4.3 mRNA 26889338 26890811 26889338 ID=MS.gene053517.t1;Parent=MS.gene053517
chr4.3 exon 26889338 26890303 26889338 ID=MS.gene053517.t1.exon1;Parent=MS.gene053517.t1
chr4.3 CDS 26889338 26890303 26889338 ID=cds.MS.gene053517.t1;Parent=MS.gene053517.t1
chr4.3 exon 26890546 26890617 26890546 ID=MS.gene053517.t1.exon2;Parent=MS.gene053517.t1
chr4.3 CDS 26890546 26890617 26890546 ID=cds.MS.gene053517.t1;Parent=MS.gene053517.t1
chr4.3 exon 26890719 26890811 26890719 ID=MS.gene053517.t1.exon3;Parent=MS.gene053517.t1
chr4.3 CDS 26890719 26890811 26890719 ID=cds.MS.gene053517.t1;Parent=MS.gene053517.t1
Gene Sequence

>MS.gene053517

ATGAACTTCGCTTGGGATGCAATGAGTGTGAAGACACCTGGAAGTGTTAATCTAGCAAGCCAGTCTTCAATATACTCCCTCACTTTTGATGAGTTACAAAGCACCATTGGGGGAGTTGGAAAAGATTTTGGATCAATGAACATGGATGAACTCTTGAAAAACATATGGAATGTTGAAGAGACCCAAGCATTGACATCTCTAACTGGTGGAGGAGTAGGAGAAGGACCTAACAACCCAAATGGTGGTAGTTTGCAAAAACAAGGCTCCTTGACATTGCCAAGGACACTTAGTCAAAGAAAAGTTGATGAAGTTTGGAGGGATTTGATGAAAGATAGTGGTTCATCTATGCCTCAAAGACAACCAACATTAGGTGAAGTGACTTTGGAGGAGTTTTTGGTGAGAGCTGGTGTTGTGAAAGAAGATACGCCTAATCATGCTCAACAAATTGAAAGACCAAATAATAATGAATGGTTTAGTGACTTCTCTAGATCAAACAATAACACCAATCTACTTGGCTTTCAACAACCAAATGGAAATAATGGGGATATGAGTGACAACAATAACTTAGTTCCTAAACATGTTCCTCTTCCTCCTTCATCAATTAACTTGAATCACTCTCAACGGCCGCCACCACTTTTCCCGAAACCTACAACTGTAGCATTTTCTTCTCCTATTCATTTGCTAAACAATGCTCAACTTGGTAACAATGGCAGAAGCGTAGTTCCTGGTGTTGGAATGCATGGTTTATCTGCCTCAAATGTCACAGCTCCTGTTACTTCTCCAGGAAGTAAAATGTCGCCGGATTTGATTACAAAGCGTAATTTAGACCCTACTTTACTGTCACCAGTTCCTTATGCAATCAATAGAGGGAGGAAGTGTGTTCCTGTAGAAAAAGGTGTTGAAAGGAGACAGAAGAGAATGATAAAGAATAGAGAATCAGCTGCGAGGTCACGGGCTCGCAAACAGGTTAGCATTTCATTTTAGTCATTTATGCATTCTTGTCAATATGAAGAAAATTTAACCATCTGTTTTGTTTCTCTGATCAGAATGATTAAGCTTGTTAAGTTCATTCGTTATTGTCCTTCAATACACATATAATGTATAACAAGATCATAAGCTTAAGGAAATTATTCAAAGTATTAAAATGATGTAAGCATTGGCTTATAATGTACATAACTTCTAAATATTTGTTTCTTTTGAATGGAAAGGCCTACACTGTTGAACTGGAGGCTGAAGTTGCAAAACTTAAGGAAGTAAACGAGGAATTACAAAGAAAACAGGTATGTTTGATTGTCATTTTGCGCTGCATTCTCATCAGTAATATGTTCACTAAGGTTTTTTTTTTTTTTTACAGTTTCTGACTACATTTGGTATGATACAGGCAGAGTTTATGGAAATTCAGAAAAGTAAGGAGGACCTGGTGCGGACAAATAAAATAAAATACTTGAGAAGGACACTTACAGGTCCATGGTAG
Protein sequence

>MS.gene053517.t1

MNFAWDAMSVKTPGSVNLASQSSIYSLTFDELQSTIGGVGKDFGSMNMDELLKNIWNVEETQALTSLTGGGVGEGPNNPNGGSLQKQGSLTLPRTLSQRKVDEVWRDLMKDSGSSMPQRQPTLGEVTLEEFLVRAGVVKEDTPNHAQQIERPNNNEWFSDFSRSNNNTNLLGFQQPNGNNGDMSDNNNLVPKHVPLPPSSINLNHSQRPPPLFPKPTTVAFSSPIHLLNNAQLGNNGRSVVPGVGMHGLSASNVTAPVTSPGSKMSPDLITKRNLDPTLLSPVPYAINRGRKCVPVEKGVERRQKRMIKNRESAARSRARKQAYTVELEAEVAKLKEVNEELQRKQAEFMEIQKSKEDLVRTNKIKYLRRTLTGPW