AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053696

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053696.t1 MTR_5g061220 92.614 528 38 1 1 527 1 528 0.0 1004
MS.gene053696.t1 MTR_1g058210 54.598 522 160 8 8 525 1 449 5.31e-176 504
MS.gene053696.t1 MTR_2g024430 49.908 545 211 14 6 525 7 514 7.90e-159 462
MS.gene053696.t1 MTR_5g040880 49.817 546 207 11 14 527 6 516 1.61e-158 462
MS.gene053696.t1 MTR_5g082140 48.362 519 191 9 14 521 7 459 5.42e-151 440
MS.gene053696.t1 MTR_5g060630 47.426 544 188 13 8 479 1 518 1.29e-142 421
MS.gene053696.t1 MTR_5g460740 47.426 544 188 13 8 479 1 518 1.29e-142 421
MS.gene053696.t1 MTR_3g073420 45.191 551 248 15 19 526 19 558 1.46e-142 423
MS.gene053696.t1 MTR_2g006270 48.162 517 184 14 15 523 9 449 4.66e-136 402
MS.gene053696.t1 MTR_5g060780 53.299 394 139 10 8 391 1 359 8.77e-129 381
MS.gene053696.t1 MTR_5g061890 53.708 391 142 9 8 391 1 359 7.35e-128 379
MS.gene053696.t1 MTR_5g460920 53.299 394 139 10 8 391 1 359 1.52e-127 378
MS.gene053696.t1 MTR_2g006380 44.015 518 191 16 19 524 20 450 1.14e-118 357
MS.gene053696.t1 MTR_5g040740 42.857 525 161 12 15 526 8 406 8.20e-117 351
MS.gene053696.t1 MTR_2g094570 39.448 507 240 15 19 477 11 498 3.97e-100 316
MS.gene053696.t1 MTR_2g094570 39.448 507 240 15 19 477 11 498 6.39e-100 317
MS.gene053696.t1 MTR_2g094570 39.448 507 240 15 19 477 67 554 1.88e-99 317
MS.gene053696.t1 MTR_1g064430 40.671 477 221 12 19 451 27 485 5.47e-99 314
MS.gene053696.t1 MTR_1g064430 40.671 477 221 12 19 451 40 498 1.04e-98 313
MS.gene053696.t1 MTR_7g101280 38.655 476 217 13 14 437 26 478 5.96e-97 307
MS.gene053696.t1 MTR_0041s0110 38.655 476 217 13 14 437 26 478 5.96e-97 307
MS.gene053696.t1 MTR_1g094960 40.889 450 216 14 19 431 19 455 2.13e-93 297
MS.gene053696.t1 MTR_4g058930 41.150 452 212 12 19 431 12 448 3.86e-93 298
MS.gene053696.t1 MTR_4g058930 41.150 452 212 12 19 431 12 448 6.98e-93 298
MS.gene053696.t1 MTR_5g062970 62.051 195 66 3 19 208 38 229 3.49e-78 245
MS.gene053696.t1 MTR_7g101275 40.426 329 154 9 29 345 2 300 8.20e-71 232
MS.gene053696.t1 MTR_5g062960 61.809 199 48 4 263 455 1 177 1.46e-70 224
MS.gene053696.t1 MTR_0041s0120 40.120 334 170 10 29 350 2 317 7.79e-70 230
MS.gene053696.t1 MTR_5g060770 41.085 387 132 12 68 437 7 314 5.97e-69 226
MS.gene053696.t1 MTR_2g014770 33.333 387 200 9 1 366 38 387 1.52e-65 224
MS.gene053696.t1 MTR_2g014770 33.773 379 194 9 10 366 44 387 2.74e-65 224
MS.gene053696.t1 MTR_2g018690 35.500 400 208 12 6 384 6 376 2.10e-60 214
MS.gene053696.t1 MTR_2g005240 35.450 378 207 7 19 385 23 374 8.03e-60 209
MS.gene053696.t1 MTR_4g088210 35.027 374 188 10 16 366 35 376 1.03e-59 209
MS.gene053696.t1 MTR_8g100050 33.943 383 207 9 19 385 59 411 1.34e-59 210
MS.gene053696.t1 MTR_4g088210 35.027 374 188 10 16 366 35 376 1.63e-59 209
MS.gene053696.t1 MTR_8g100050 33.943 383 207 9 19 385 59 411 5.02e-59 209
MS.gene053696.t1 MTR_4g124900 34.140 372 196 7 14 368 18 357 7.31e-59 211
MS.gene053696.t1 MTR_4g124900 34.140 372 196 7 14 368 18 357 9.12e-59 211
MS.gene053696.t1 MTR_5g076270 34.974 386 199 11 19 384 24 377 9.24e-59 209
MS.gene053696.t1 MTR_2g093740 33.418 392 209 11 18 388 55 415 1.20e-58 206
MS.gene053696.t1 MTR_3g064050 33.766 385 205 9 19 384 26 379 2.22e-58 208
MS.gene053696.t1 MTR_4g021580 35.171 381 205 10 19 385 19 371 2.33e-58 204
MS.gene053696.t1 MTR_4g021580 35.171 381 205 10 19 385 19 371 2.90e-58 205
MS.gene053696.t1 MTR_2g093740 33.418 392 209 11 18 388 55 415 3.06e-58 206
MS.gene053696.t1 MTR_2g093740 33.418 392 209 11 18 388 55 415 6.52e-58 206
MS.gene053696.t1 MTR_2g043250 34.146 369 200 7 14 368 18 357 7.54e-58 208
MS.gene053696.t1 MTR_1g024025 35.262 363 191 9 19 366 43 376 9.12e-57 205
MS.gene053696.t1 MTR_8g079492 33.915 401 212 12 6 384 6 375 1.00e-56 204
MS.gene053696.t1 MTR_8g079492 33.915 401 212 12 6 384 6 375 1.03e-56 204
MS.gene053696.t1 MTR_8g101360 34.239 368 201 9 16 369 28 368 1.78e-55 201
MS.gene053696.t1 MTR_7g062540 31.626 449 260 14 19 448 14 434 2.40e-55 197
MS.gene053696.t1 MTR_2g093740 32.346 405 209 12 18 388 55 428 4.24e-55 197
MS.gene053696.t1 MTR_2g093740 32.346 405 209 12 18 388 55 428 1.07e-54 197
MS.gene053696.t1 MTR_2g093740 32.346 405 209 12 18 388 55 428 1.69e-54 197
MS.gene053696.t1 MTR_4g060460 33.417 398 201 10 18 385 53 416 5.01e-53 192
MS.gene053696.t1 MTR_4g060460 33.417 398 201 10 18 385 53 416 6.13e-53 192
MS.gene053696.t1 MTR_8g027440 33.598 378 211 9 19 385 29 377 2.61e-52 189
MS.gene053696.t1 MTR_5g460910 36.585 410 135 16 51 437 2 309 7.44e-51 177
MS.gene053696.t1 MTR_8g079492 33.648 318 172 8 79 384 3 293 1.27e-43 166
MS.gene053696.t1 MTR_2g093740 32.588 313 166 8 90 388 4 285 6.07e-42 159
MS.gene053696.t1 MTR_2g093740 32.588 313 166 8 90 388 4 285 1.05e-41 159
MS.gene053696.t1 MTR_2g093740 31.481 324 164 9 92 388 6 298 1.57e-38 149
MS.gene053696.t1 MTR_2g093740 31.288 326 166 9 90 388 4 298 2.18e-38 149
MS.gene053696.t1 MTR_8g446900 28.197 305 181 7 70 366 14 288 3.07e-31 125
MS.gene053696.t1 MTR_5g046120 39.130 115 57 4 57 163 12 121 1.61e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053696.t1 AT1G77850 38.783 575 285 14 1 526 1 557 6.93e-121 367
MS.gene053696.t1 AT1G77850 38.783 575 285 14 1 526 1 557 9.54e-121 367
MS.gene053696.t1 AT4G30080 39.279 555 272 21 19 526 19 555 2.20e-95 304
MS.gene053696.t1 AT2G28350 42.327 404 190 11 19 388 10 404 3.02e-87 283
MS.gene053696.t1 AT2G28350 42.327 404 190 11 19 388 10 404 3.38e-87 283
MS.gene053696.t1 AT2G46530 36.769 359 193 8 19 369 21 353 3.19e-63 217
MS.gene053696.t1 AT2G46530 36.798 356 191 8 19 366 42 371 5.40e-63 217
MS.gene053696.t1 AT2G33860 34.865 370 190 8 16 366 51 388 1.05e-62 216
MS.gene053696.t1 AT5G37020 34.000 400 213 11 19 399 22 389 3.04e-61 215
MS.gene053696.t1 AT5G37020 34.000 400 213 11 19 399 22 389 5.70e-61 215
MS.gene053696.t1 AT3G61830 35.040 371 197 9 19 381 25 359 1.81e-60 210
MS.gene053696.t1 AT1G30330 34.197 386 212 8 14 384 20 378 1.96e-60 214
MS.gene053696.t1 AT1G30330 34.197 386 212 8 14 384 20 378 1.96e-60 214
MS.gene053696.t1 AT1G30330 34.197 386 212 8 14 384 18 376 1.99e-60 214
MS.gene053696.t1 AT4G23980 30.103 485 284 13 19 483 12 461 3.47e-57 201
MS.gene053696.t1 AT5G60450 34.271 391 203 13 13 382 35 392 4.66e-57 203
MS.gene053696.t1 AT5G62000 32.921 404 215 10 3 385 45 413 6.67e-57 204
MS.gene053696.t1 AT4G23980 33.333 384 214 9 19 389 12 366 7.82e-57 201
MS.gene053696.t1 AT5G62000 32.921 404 215 10 3 385 45 413 9.04e-57 204
MS.gene053696.t1 AT5G62000 32.921 404 215 10 3 385 45 413 9.04e-57 204
MS.gene053696.t1 AT5G62000 32.921 404 215 10 3 385 45 413 9.04e-57 204
MS.gene053696.t1 AT5G62000 32.921 404 215 10 3 385 45 413 9.04e-57 204
MS.gene053696.t1 AT5G60450 34.545 385 198 13 19 382 65 416 1.05e-56 202
MS.gene053696.t1 AT5G20730 33.957 374 194 8 14 368 19 358 1.45e-56 204
MS.gene053696.t1 AT1G59750 33.884 363 204 6 19 371 22 358 1.80e-56 200
MS.gene053696.t1 AT1G59750 33.884 363 204 6 19 371 22 358 1.83e-56 200
MS.gene053696.t1 AT1G59750 33.884 363 204 6 19 371 22 358 2.35e-56 200
MS.gene053696.t1 AT1G59750 33.884 363 204 6 19 371 22 358 2.35e-56 200
MS.gene053696.t1 AT1G19220 33.850 387 208 11 16 385 20 375 1.32e-55 201
MS.gene053696.t1 AT1G34310 33.508 382 211 9 19 389 27 376 1.52e-55 196
MS.gene053696.t1 AT5G20730 33.867 375 194 9 14 368 19 359 4.78e-55 200
MS.gene053696.t1 AT5G20730 33.867 375 194 9 14 368 19 359 5.00e-55 200
MS.gene053696.t1 AT1G35520 34.204 383 207 11 19 389 27 376 6.33e-55 194
MS.gene053696.t1 AT1G34410 32.323 396 226 10 19 405 27 389 9.52e-55 194
MS.gene053696.t1 AT1G34390 32.558 387 214 10 19 397 27 374 1.57e-54 194
MS.gene053696.t1 AT1G19850 34.153 366 198 9 16 366 40 377 4.68e-54 196
MS.gene053696.t1 AT1G19850 34.153 366 198 9 16 366 51 388 6.54e-54 196
MS.gene053696.t1 AT1G35240 32.741 394 213 12 19 398 27 382 5.76e-52 186
MS.gene053696.t1 AT1G35540 32.105 380 219 9 19 389 27 376 6.70e-52 186
MS.gene053696.t1 AT1G34170 31.421 366 195 10 19 366 25 352 7.95e-47 171
MS.gene053696.t1 AT3G61830 34.036 332 179 8 54 381 13 308 1.17e-46 171
MS.gene053696.t1 AT2G33860 34.241 257 135 4 117 366 2 231 3.59e-45 165
MS.gene053696.t1 AT2G46530 36.965 257 137 4 114 369 34 266 6.80e-44 163
MS.gene053696.t1 AT5G37020 34.589 292 154 8 117 399 31 294 3.82e-42 160
MS.gene053696.t1 AT1G34170 27.115 520 269 17 19 507 25 465 3.40e-41 156
MS.gene053696.t1 AT1G34170 29.781 366 175 10 19 366 25 326 1.87e-40 152
MS.gene053696.t1 AT1G43950 38.725 204 111 5 19 216 27 222 1.25e-37 138

Find 150 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTTCAATTAAGTTATT+TGG 0.154755 5.1:-46990411 MS.gene053696:CDS
TTCAAACTACTAAGGTTATT+TGG 0.200926 5.1:+46990489 None:intergenic
TCTCTTTATCATAGGTTAAC+TGG 0.215844 5.1:-46990866 MS.gene053696:intron
CCCTAATTGGCGCATGCTTC+AGG 0.273598 5.1:-46992636 MS.gene053696:intron
AGAGAATGGTTTGGAGGTTA+AGG 0.274717 5.1:-46992939 MS.gene053696:CDS
CATGATCATTTATCTCTATC+AGG 0.276134 5.1:-46990597 MS.gene053696:CDS
TGAACCAAACATTGTTGAAT+TGG 0.283162 5.1:-46990653 MS.gene053696:CDS
TATATTACCCTAGGGCTAAT+TGG 0.305719 5.1:-46992817 MS.gene053696:CDS
AATTTCATTGGATCCTTTAG+AGG 0.319231 5.1:+46990329 None:intergenic
CAAACAATCGCCGTTATTGA+TGG 0.325853 5.1:-46993484 MS.gene053696:CDS
GTCAATGCATCAAATTTCAT+TGG 0.331745 5.1:+46990317 None:intergenic
CTGATGTTCATGACGGAGTT+TGG 0.334825 5.1:-46993171 MS.gene053696:CDS
TACCGGACTGAGTAGCAATT+TGG 0.335469 5.1:+46993387 None:intergenic
AGCCCCAATTCAACAATGTT+TGG 0.335857 5.1:+46990649 None:intergenic
ACGGCGATTGTTTGAGCGTT+AGG 0.342143 5.1:+46993493 None:intergenic
ATGAAGATTGGTTGGGCATC+TGG 0.343084 5.1:-46992755 MS.gene053696:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.343626 5.1:+46992635 None:intergenic
GAACCAAACATTGTTGAATT+GGG 0.349855 5.1:-46990652 MS.gene053696:CDS
CTTAGTAGTTTGAACACATT+CGG 0.352705 5.1:-46990480 MS.gene053696:CDS
GAGAATGGTTTGGAGGTTAA+GGG 0.354789 5.1:-46992938 MS.gene053696:CDS
GAAAGTGATGTCCATGATTC+TGG 0.371511 5.1:-46990369 MS.gene053696:CDS
GGAGAATTTAAGTCAAGCTT+GGG 0.377595 5.1:+46993220 None:intergenic
CCTGAAGCATGCGCCAATTA+GGG 0.379672 5.1:+46992636 None:intergenic
TAAACTTTAGAACGTACCTT+TGG 0.384455 5.1:+46993556 None:intergenic
TGCCTCAATAACCGCCTTCT+CGG 0.388165 5.1:+46992877 None:intergenic
GAGTCCAACCAGTAGTAAAA+AGG 0.388502 5.1:+46993110 None:intergenic
TCTTTGAATGATAATAGTTA+TGG 0.390167 5.1:-46990564 MS.gene053696:CDS
CGGCGATTGTTTGAGCGTTA+GGG 0.395501 5.1:+46993494 None:intergenic
GGAGTTGGCGGAAAAGAATA+TGG 0.402969 5.1:-46992852 MS.gene053696:CDS
CTCTTTATCATAGGTTAACT+GGG 0.403608 5.1:-46990865 MS.gene053696:intron
GGGACAGACATGGTGGCCGG+TGG 0.403787 5.1:+46993640 None:intergenic
TAGATGCACTGCAGGATTGC+GGG 0.405787 5.1:+46990782 None:intergenic
CAGCAGGCATGCAGGGAGTC+AGG 0.416086 5.1:-46990620 MS.gene053696:CDS
TTGAATTTCACGCGGCGGGC+CGG 0.416798 5.1:+46993616 None:intergenic
AGTTCTTGTCCTACAGTTAC+CGG 0.417820 5.1:+46993370 None:intergenic
GCCGCGTGAAATTCAAAATC+TGG 0.417967 5.1:-46993609 MS.gene053696:CDS
ATGTGTCCAGTGTCCCTAAT+TGG 0.419261 5.1:-46992649 MS.gene053696:CDS
TTAGTAGTTTGAACACATTC+GGG 0.421729 5.1:-46990479 MS.gene053696:CDS
ACTATTATCATTCAAAGAGT+TGG 0.428670 5.1:+46990569 None:intergenic
ATCAAAACCGCGTCAATCCT+TGG 0.437926 5.1:-46990821 MS.gene053696:CDS
ATCAACGGCCGTGATGATAC+AGG 0.446735 5.1:+46993444 None:intergenic
TTGAAGTTATATATTACCCT+AGG 0.447607 5.1:-46992826 MS.gene053696:CDS
AGGAGAATTTAAGTCAAGCT+TGG 0.457295 5.1:+46993219 None:intergenic
CGCCAAATTGCTACTCAGTC+CGG 0.457882 5.1:-46993389 MS.gene053696:CDS
CTGACTCCCTGCATGCCTGC+TGG 0.459021 5.1:+46990621 None:intergenic
ACAAAATCTGATTCTAATAA+CGG 0.462788 5.1:-46993286 MS.gene053696:CDS
AAGGAGGAGGGTAAAGAGAA+TGG 0.463485 5.1:-46992953 MS.gene053696:CDS
GAACTCTATCATAGGAATGA+AGG 0.464627 5.1:+46990689 None:intergenic
GGTTTGGAGGTTAAGGGAGA+TGG 0.469959 5.1:-46992932 MS.gene053696:CDS
TCTTCTTCATCAACAACCTC+GGG 0.472000 5.1:+46993346 None:intergenic
CTCTGAGGGAACTCTATCAT+AGG 0.472447 5.1:+46990681 None:intergenic
AACACAAAAGGAGTTGGTTC+TGG 0.474124 5.1:-46990429 MS.gene053696:CDS
GGAGGGTAAAGAGAATGGTT+TGG 0.480150 5.1:-46992948 MS.gene053696:CDS
AATTTCACGCGGCGGGCCGG+AGG 0.480942 5.1:+46993619 None:intergenic
GGCCACCATGTCTGTCCCTC+CGG 0.483118 5.1:-46993635 MS.gene053696:CDS
GTGGATGATGCTATGAAGAT+TGG 0.483610 5.1:-46992767 MS.gene053696:CDS
AAGGGTGAAGATTCCTCTAA+AGG 0.485287 5.1:-46990342 MS.gene053696:CDS
TTTAGAGGAATCTTCACCCT+TGG 0.485917 5.1:+46990344 None:intergenic
GGTTATTGAGGCAGTGGAGT+TGG 0.491818 5.1:-46992867 MS.gene053696:CDS
TTACTTCCCACAAGGTCACT+TGG 0.494429 5.1:-46993533 MS.gene053696:CDS
TGGAACGTTCACCGATGGAC+CGG 0.495600 5.1:+46993576 None:intergenic
CGAATGTGTTCAAACTACTA+AGG 0.499098 5.1:+46990481 None:intergenic
ACCTTTGGAACGTTCACCGA+TGG 0.510835 5.1:+46993571 None:intergenic
TTCTTCTTCATCAACAACCT+CGG 0.514354 5.1:+46993345 None:intergenic
AAAATCTGGCAGACATGTGC+CGG 0.515291 5.1:-46993595 MS.gene053696:CDS
GGACAGACATGGTGGCCGGT+GGG 0.516458 5.1:+46993641 None:intergenic
GCAGACATGTGCCGGTCCAT+CGG 0.518088 5.1:-46993587 MS.gene053696:CDS
GTGAATAATAAGAAGGAGGA+GGG 0.519419 5.1:-46992965 MS.gene053696:CDS
AAAAGCAGTGAATAATAAGA+AGG 0.519919 5.1:-46992972 MS.gene053696:CDS
ATTGTTTGAGCGTTAGGGGA+AGG 0.521449 5.1:+46993499 None:intergenic
ACTGCCGGAGATATAGTTGT+TGG 0.521728 5.1:-46993031 MS.gene053696:CDS
AACCGAGAAGGCGGTTATTG+AGG 0.522496 5.1:-46992879 MS.gene053696:CDS
ACTCACAACAACAACACAAA+AGG 0.522870 5.1:-46990441 MS.gene053696:CDS
TCTGATTCTAATAACGGCGG+TGG 0.523119 5.1:-46993280 MS.gene053696:CDS
GAAGATTCATCAATCTTCAA+AGG 0.524456 5.1:+46992716 None:intergenic
GTAGAATCCTGAACTAGTCT+AGG 0.524565 5.1:+46990738 None:intergenic
AGTGAATAATAAGAAGGAGG+AGG 0.524591 5.1:-46992966 MS.gene053696:CDS
GAGAGTTCCTGAGACGGAAA+AGG 0.524822 5.1:+46993199 None:intergenic
GACAGACATGGTGGCCGGTG+GGG 0.526151 5.1:+46993642 None:intergenic
GGGTAAAGAGAATGGTTTGG+AGG 0.526164 5.1:-46992945 MS.gene053696:CDS
CACCCTTGGTACCAGAATCA+TGG 0.526655 5.1:+46990358 None:intergenic
CAGCAAACTTCATGTTTCTG+CGG 0.530328 5.1:+46993005 None:intergenic
GGTGGCCGGTGGGGAAGACA+TGG 0.530680 5.1:+46993651 None:intergenic
GACCTCATCGGTGTGAGGAT+CGG 0.532501 5.1:+46993414 None:intergenic
TGTAGTTGATTCAAATGTTG+TGG 0.533977 5.1:-46992786 MS.gene053696:CDS
GGAGGGACAGACATGGTGGC+CGG 0.536529 5.1:+46993637 None:intergenic
AACCAAACATTGTTGAATTG+GGG 0.537810 5.1:-46990651 MS.gene053696:CDS
AACTCTATCATAGGAATGAA+GGG 0.541293 5.1:+46990690 None:intergenic
TTCCTTTCCCTGTATCATCA+CGG 0.541294 5.1:-46993452 MS.gene053696:CDS
AGAAATGGTAGATGCACTGC+AGG 0.541883 5.1:+46990774 None:intergenic
GATTCCAACAACTATATCTC+CGG 0.544415 5.1:+46993027 None:intergenic
CATGTTCCAAGTGACCTTGT+GGG 0.545385 5.1:+46993527 None:intergenic
TTGTTTGAGCGTTAGGGGAA+GGG 0.546169 5.1:+46993500 None:intergenic
TACCTTGTTTAGTGATAACA+AGG 0.546334 5.1:-46990718 MS.gene053696:CDS
TATTGAGGCAGTGGAGTTGG+CGG 0.551866 5.1:-46992864 MS.gene053696:CDS
GAAATAAGTTCAACTTGCCA+AGG 0.552664 5.1:+46990804 None:intergenic
GAAGGCGGTTATTGAGGCAG+TGG 0.558765 5.1:-46992873 MS.gene053696:CDS
TTGAGCGTTAGGGGAAGGGC+AGG 0.559568 5.1:+46993504 None:intergenic
ATGATGCTATGAAGATTGGT+TGG 0.562627 5.1:-46992763 MS.gene053696:CDS
TTTGGTTCAAAGATCTCTGA+GGG 0.562864 5.1:+46990667 None:intergenic
TGAAGTTATATATTACCCTA+GGG 0.565837 5.1:-46992825 MS.gene053696:CDS
TGGCGACAAGTCGTTAGATG+TGG 0.568778 5.1:+46990509 None:intergenic
GGCGATTGTTTGAGCGTTAG+GGG 0.568994 5.1:+46993495 None:intergenic
GCATGTTCCAAGTGACCTTG+TGG 0.569473 5.1:+46993526 None:intergenic
AAATTGCACCAATTAGCCCT+AGG 0.571867 5.1:+46992809 None:intergenic
ATCATGGACATCACTTTCAA+CGG 0.572291 5.1:+46990374 None:intergenic
TTCCACCATGTCTTCCCCAC+CGG 0.577652 5.1:-46993656 None:intergenic
TGATGCTATGAAGATTGGTT+GGG 0.580467 5.1:-46992762 MS.gene053696:CDS
GGCCGTGATGATACAGGGAA+AGG 0.585344 5.1:+46993450 None:intergenic
ATTTCACGCGGCGGGCCGGA+GGG 0.586809 5.1:+46993620 None:intergenic
AAGGGGAATGCTAACCGAGA+AGG 0.592212 5.1:-46992891 MS.gene053696:CDS
GTAGATGCACTGCAGGATTG+CGG 0.593419 5.1:+46990781 None:intergenic
GGCGACAAGTCGTTAGATGT+GGG 0.596585 5.1:+46990510 None:intergenic
AACAACAACACAAAAGGAGT+TGG 0.596832 5.1:-46990435 MS.gene053696:CDS
TGTCCATGATTCTGGTACCA+AGG 0.599100 5.1:-46990361 MS.gene053696:CDS
CATGCGCCAATTAGGGACAC+TGG 0.600814 5.1:+46992643 None:intergenic
CTACTCAGTCCGGTAACTGT+AGG 0.600878 5.1:-46993379 MS.gene053696:CDS
GTGATGATACAGGGAAAGGA+AGG 0.601435 5.1:+46993454 None:intergenic
TCCATCGGTGAACGTTCCAA+AGG 0.605082 5.1:-46993572 MS.gene053696:CDS
AAGTTTGCTCATGTCTATCG+AGG 0.613801 5.1:-46993148 MS.gene053696:CDS
GTCCATGATTCTGGTACCAA+GGG 0.620974 5.1:-46990360 MS.gene053696:CDS
TCAACGGCCGTGATGATACA+GGG 0.621862 5.1:+46993445 None:intergenic
GCGGGCCGGAGGGACAGACA+TGG 0.622803 5.1:+46993630 None:intergenic
AGGACGGTAACCATCAATAA+CGG 0.623715 5.1:+46993474 None:intergenic
ACCCTTGTTATCACTAAACA+AGG 0.625546 5.1:+46990716 None:intergenic
CAACTTGCCAAGGATTGACG+CGG 0.626664 5.1:+46990814 None:intergenic
AGCAGTGAATAATAAGAAGG+AGG 0.629221 5.1:-46992969 MS.gene053696:CDS
CAATTTGGCGAAGACCTCAT+CGG 0.632488 5.1:+46993402 None:intergenic
AATTGCACCAATTAGCCCTA+GGG 0.639117 5.1:+46992810 None:intergenic
ATCAAGTCAGCACAAAGGCG+AGG 0.645484 5.1:+46993247 None:intergenic
GTTTGGTTCAAAGATCTCTG+AGG 0.646990 5.1:+46990666 None:intergenic
TCCGTCATGAACATCAGTAA+CGG 0.649838 5.1:+46993177 None:intergenic
GCGAAGACCTCATCGGTGTG+AGG 0.652471 5.1:+46993409 None:intergenic
GGGAATGCTAACCGAGAAGG+CGG 0.652518 5.1:-46992888 MS.gene053696:CDS
AGTTTGCTCATGTCTATCGA+GGG 0.654001 5.1:-46993147 MS.gene053696:CDS
TCCGTTACTGATGTTCATGA+CGG 0.654864 5.1:-46993178 MS.gene053696:CDS
CATCGGTGTGAGGATCGGCT+AGG 0.655915 5.1:+46993419 None:intergenic
GTTGTTGATGAAGAAGAAGA+CGG 0.659219 5.1:-46993340 MS.gene053696:CDS
AGCCGATCCTCACACCGATG+AGG 0.659256 5.1:-46993416 MS.gene053696:CDS
GGCCGGAGGGACAGACATGG+TGG 0.661369 5.1:+46993633 None:intergenic
AAAGTTTATTACTTCCCACA+AGG 0.663461 5.1:-46993541 MS.gene053696:CDS
ACATTAGAGATTGTTCCTTG+AGG 0.666803 5.1:+46992668 None:intergenic
TGATACAGGGAAAGGAAGGA+CGG 0.682674 5.1:+46993458 None:intergenic
GTAACGGAGAGTTCCTGAGA+CGG 0.687969 5.1:+46993193 None:intergenic
GAGAATTTAAGTCAAGCTTG+GGG 0.690174 5.1:+46993221 None:intergenic
AGGATCGGCTAGGAGATCAA+CGG 0.692255 5.1:+46993429 None:intergenic
AAATCTGATTCTAATAACGG+CGG 0.706742 5.1:-46993283 MS.gene053696:CDS
GGAAGATCAAGTCAGCACAA+AGG 0.715164 5.1:+46993242 None:intergenic
ACCTTGTTTAGTGATAACAA+GGG 0.724822 5.1:-46990717 MS.gene053696:CDS
TGTAGGACAAGAACTTCCCG+AGG 0.729698 5.1:-46993362 MS.gene053696:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTAGTATATAAATATT+AGG - chr5.1:46992419-46992438 MS.gene053696:intron 10.0%
!!! AGAATACTTTTTTTTTTTTT+TGG - chr5.1:46992641-46992660 MS.gene053696:CDS 10.0%
!!! TATTTTATTTTTTGAATCTA+GGG - chr5.1:46991392-46991411 MS.gene053696:intron 10.0%
!!! TGCATTTTATTATAAAAATA+TGG + chr5.1:46992951-46992970 None:intergenic 10.0%
!!! TTATTTTATTTTTTGAATCT+AGG - chr5.1:46991391-46991410 MS.gene053696:intron 10.0%
!! ATAGTTTAGTATACTTAATT+AGG + chr5.1:46991607-46991626 None:intergenic 15.0%
!! TTATATACTAAATTGATCAA+GGG + chr5.1:46992414-46992433 None:intergenic 15.0%
!! TTTATATACTAAATTGATCA+AGG + chr5.1:46992415-46992434 None:intergenic 15.0%
!!! AAAACATGATTTTTTAAACT+TGG + chr5.1:46992389-46992408 None:intergenic 15.0%
!!! ATTTTACATTAATAATTGCT+TGG + chr5.1:46992067-46992086 None:intergenic 15.0%
!!! CTATAATTATGGATTAATTT+TGG + chr5.1:46992731-46992750 None:intergenic 15.0%
!! AAATATTAGGGTTAAGTTAT+AGG - chr5.1:46992432-46992451 MS.gene053696:intron 20.0%
!! ACAAAATCTGATTCTAATAA+CGG - chr5.1:46990689-46990708 MS.gene053696:CDS 20.0%
!! CTATGATAAAGAGATAAAAA+TGG + chr5.1:46993103-46993122 None:intergenic 20.0%
!! TATTTATAACTTATCCGATA+GGG + chr5.1:46992487-46992506 None:intergenic 20.0%
!! TCTTTGAATGATAATAGTTA+TGG - chr5.1:46993411-46993430 MS.gene053696:CDS 20.0%
!! TTATTTATAACTTATCCGAT+AGG + chr5.1:46992488-46992507 None:intergenic 20.0%
!! TTGAAACTATAATCAAATGA+TGG - chr5.1:46992011-46992030 MS.gene053696:intron 20.0%
!!! CATTTTTATCTCTTTATCAT+AGG - chr5.1:46993101-46993120 MS.gene053696:CDS 20.0%
!!! GTCGTTAATTTACTATTTTT+TGG - chr5.1:46992538-46992557 MS.gene053696:intron 20.0%
!!! TGGAAAAGTATTAGATATTT+AGG - chr5.1:46992922-46992941 MS.gene053696:CDS 20.0%
! AAAAGCAGTGAATAATAAGA+AGG - chr5.1:46991003-46991022 MS.gene053696:intron 25.0%
! AAATTAACGACTAACTTAGT+TGG + chr5.1:46992529-46992548 None:intergenic 25.0%
! AACATTATTCAACGATTATG+AGG - chr5.1:46992141-46992160 MS.gene053696:intron 25.0%
! ACTATTATCATTCAAAGAGT+TGG + chr5.1:46993409-46993428 None:intergenic 25.0%
! AGTATGTATATGCACATAAA+AGG + chr5.1:46993038-46993057 None:intergenic 25.0%
! ATGATTACCAAAATACAGTT+AGG - chr5.1:46992165-46992184 MS.gene053696:intron 25.0%
! ATGTTTAATCAAGAGGAATA+GGG - chr5.1:46992298-46992317 MS.gene053696:intron 25.0%
! ATTTACTCTTATTCCTAAGT+AGG - chr5.1:46992970-46992989 MS.gene053696:CDS 25.0%
! GAAAAGTGAGTGTAAAAAAA+AGG + chr5.1:46991699-46991718 None:intergenic 25.0%
! GGTATATATGTTTAATCAAG+AGG - chr5.1:46992291-46992310 MS.gene053696:intron 25.0%
! GTAAAATATAGATCCTACTT+AGG + chr5.1:46992986-46993005 None:intergenic 25.0%
! TAAAGAGAAAGAAAAATCTC+CGG + chr5.1:46991818-46991837 None:intergenic 25.0%
! TACCTTCAACACTAATAAAA+GGG - chr5.1:46992574-46992593 MS.gene053696:intron 25.0%
! TATGTTTAATCAAGAGGAAT+AGG - chr5.1:46992297-46992316 MS.gene053696:intron 25.0%
! TATTATTGTCGAAGAAGTTT+AGG - chr5.1:46991446-46991465 MS.gene053696:intron 25.0%
! TGAAGTTATATATTACCCTA+GGG - chr5.1:46991150-46991169 MS.gene053696:intron 25.0%
! TTACCTTCAACACTAATAAA+AGG - chr5.1:46992573-46992592 MS.gene053696:intron 25.0%
! TTCAAACTACTAAGGTTATT+TGG + chr5.1:46993489-46993508 None:intergenic 25.0%
! TTGAAGTTATATATTACCCT+AGG - chr5.1:46991149-46991168 MS.gene053696:intron 25.0%
! TTGCAGTTGATCTATAATTA+TGG + chr5.1:46992742-46992761 None:intergenic 25.0%
!! AGTGTAAAAAAAAGGTTTTC+GGG + chr5.1:46991691-46991710 None:intergenic 25.0%
!! ATTGCTTGGTATTAGAATTT+TGG + chr5.1:46992053-46992072 None:intergenic 25.0%
!! GAGTGTAAAAAAAAGGTTTT+CGG + chr5.1:46991692-46991711 None:intergenic 25.0%
!! GATTTTTCCTAACTGTATTT+TGG + chr5.1:46992175-46992194 None:intergenic 25.0%
!! TCTGGTTCAATTAAGTTATT+TGG - chr5.1:46993564-46993583 MS.gene053696:CDS 25.0%
!! TTTTGTAAGAGTCTTTACAA+AGG + chr5.1:46990675-46990694 None:intergenic 25.0%
!!! ATATTTTGAATGACTGATGT+TGG - chr5.1:46993070-46993089 MS.gene053696:CDS 25.0%
!!! GTTTTCAGGCATATAAATAA+TGG - chr5.1:46992879-46992898 MS.gene053696:CDS 25.0%
AAAGTTTATTACTTCCCACA+AGG - chr5.1:46990434-46990453 MS.gene053696:CDS 30.0%
AAATCTGATTCTAATAACGG+CGG - chr5.1:46990692-46990711 MS.gene053696:CDS 30.0%
AACCAAACATTGTTGAATTG+GGG - chr5.1:46993324-46993343 MS.gene053696:CDS 30.0%
AACTCTATCATAGGAATGAA+GGG + chr5.1:46993288-46993307 None:intergenic 30.0%
AATTTCATTGGATCCTTTAG+AGG + chr5.1:46993649-46993668 None:intergenic 30.0%
ACAAAATTTATTTGCTCCAC+CGG - chr5.1:46991796-46991815 MS.gene053696:intron 30.0%
ACTAATACCCATGTAATTCA+TGG + chr5.1:46991906-46991925 None:intergenic 30.0%
CATGATCATTTATCTCTATC+AGG - chr5.1:46993378-46993397 MS.gene053696:CDS 30.0%
CTCTTATTCAACCTCTTAAT+TGG - chr5.1:46991839-46991858 MS.gene053696:intron 30.0%
CTCTTTATCATAGGTTAACT+GGG - chr5.1:46993110-46993129 MS.gene053696:CDS 30.0%
CTTAGTAGTTTGAACACATT+CGG - chr5.1:46993495-46993514 MS.gene053696:CDS 30.0%
GAACCAAACATTGTTGAATT+GGG - chr5.1:46993323-46993342 MS.gene053696:CDS 30.0%
GAAGATTCATCAATCTTCAA+AGG + chr5.1:46991262-46991281 None:intergenic 30.0%
TAGGGAAGTTATAGATAACT+TGG - chr5.1:46992316-46992335 MS.gene053696:intron 30.0%
TCAAGAGAATTATGTGACTA+TGG - chr5.1:46991988-46992007 MS.gene053696:intron 30.0%
TCTCTTTATCATAGGTTAAC+TGG - chr5.1:46993109-46993128 MS.gene053696:CDS 30.0%
TGAACCAAACATTGTTGAAT+TGG - chr5.1:46993322-46993341 MS.gene053696:CDS 30.0%
TTAGTAGTTTGAACACATTC+GGG - chr5.1:46993496-46993515 MS.gene053696:CDS 30.0%
TTGTGAATTGATACTTGTAC+CGG + chr5.1:46991957-46991976 None:intergenic 30.0%
! AAAAGACACCTTTTTACTAC+TGG - chr5.1:46990857-46990876 MS.gene053696:intron 30.0%
! ACCTTGTTTAGTGATAACAA+GGG - chr5.1:46993258-46993277 MS.gene053696:CDS 30.0%
! ATAAATAATGGAGAAGCTTC+AGG - chr5.1:46992891-46992910 MS.gene053696:CDS 30.0%
! GTGGAGCAAATAAATTTTGT+TGG + chr5.1:46991796-46991815 None:intergenic 30.0%
! GTTGAATAATGTTTTCAGCA+AGG + chr5.1:46992134-46992153 None:intergenic 30.0%
! TAAATAATGGAGAAGCTTCA+GGG - chr5.1:46992892-46992911 MS.gene053696:CDS 30.0%
! TACCTTGTTTAGTGATAACA+AGG - chr5.1:46993257-46993276 MS.gene053696:CDS 30.0%
! TGTAGTTGATTCAAATGTTG+TGG - chr5.1:46991189-46991208 MS.gene053696:intron 30.0%
!! TAAACTTTAGAACGTACCTT+TGG + chr5.1:46990422-46990441 None:intergenic 30.0%
!! TTCCCTTTTATTAGTGTTGA+AGG + chr5.1:46992579-46992598 None:intergenic 30.0%
!!! AGTAGTAAAAAGGTGTCTTT+TGG + chr5.1:46990858-46990877 None:intergenic 30.0%
!!! AGTTTTTTCGTGTTCACAAA+CGG + chr5.1:46990890-46990909 None:intergenic 30.0%
!!! GTAGTAAAAAGGTGTCTTTT+GGG + chr5.1:46990857-46990876 None:intergenic 30.0%
!!! TCTTTTTTAGAGCTTCAAAG+AGG - chr5.1:46991766-46991785 MS.gene053696:intron 30.0%
AACAACAACACAAAAGGAGT+TGG - chr5.1:46993540-46993559 MS.gene053696:CDS 35.0%
AAGAATTCGATGCATGCATA+CGG - chr5.1:46991543-46991562 MS.gene053696:intron 35.0%
AATCATATGAGGAGCAAGAA+GGG + chr5.1:46992820-46992839 None:intergenic 35.0%
ACATTAGAGATTGTTCCTTG+AGG + chr5.1:46991310-46991329 None:intergenic 35.0%
ACTCACAACAACAACACAAA+AGG - chr5.1:46993534-46993553 MS.gene053696:CDS 35.0%
AGCAGTGAATAATAAGAAGG+AGG - chr5.1:46991006-46991025 MS.gene053696:intron 35.0%
AGGAGAATTTAAGTCAAGCT+TGG + chr5.1:46990759-46990778 None:intergenic 35.0%
AGTGAATAATAAGAAGGAGG+AGG - chr5.1:46991009-46991028 MS.gene053696:intron 35.0%
ATCAAAGAGGACCAATTAAG+AGG + chr5.1:46991853-46991872 None:intergenic 35.0%
ATCATGGACATCACTTTCAA+CGG + chr5.1:46993604-46993623 None:intergenic 35.0%
ATGATGCTATGAAGATTGGT+TGG - chr5.1:46991212-46991231 MS.gene053696:intron 35.0%
CAATACTATGAATCGATGCA+AGG + chr5.1:46991578-46991597 None:intergenic 35.0%
CCAATCTCCATGAATTACAT+GGG - chr5.1:46991896-46991915 MS.gene053696:intron 35.0%
CGAATGTGTTCAAACTACTA+AGG + chr5.1:46993497-46993516 None:intergenic 35.0%
GAAATAAGTTCAACTTGCCA+AGG + chr5.1:46993174-46993193 None:intergenic 35.0%
GAACTCTATCATAGGAATGA+AGG + chr5.1:46993289-46993308 None:intergenic 35.0%
GAGAATTTAAGTCAAGCTTG+GGG + chr5.1:46990757-46990776 None:intergenic 35.0%
GATTCCAACAACTATATCTC+CGG + chr5.1:46990951-46990970 None:intergenic 35.0%
GGAGAATTTAAGTCAAGCTT+GGG + chr5.1:46990758-46990777 None:intergenic 35.0%
GTCAATCACTCAATCATATG+AGG + chr5.1:46992831-46992850 None:intergenic 35.0%
GTGAATAATAAGAAGGAGGA+GGG - chr5.1:46991010-46991029 MS.gene053696:intron 35.0%
TATATTACCCTAGGGCTAAT+TGG - chr5.1:46991158-46991177 MS.gene053696:intron 35.0%
TTCTTCTTCATCAACAACCT+CGG + chr5.1:46990633-46990652 None:intergenic 35.0%
! AAAAAAGCCTAGACTAGTTC+AGG - chr5.1:46993230-46993249 MS.gene053696:CDS 35.0%
! AAAGAGTGACAAGTGTTTTC+AGG - chr5.1:46992865-46992884 MS.gene053696:CDS 35.0%
! ACCCTTGTTATCACTAAACA+AGG + chr5.1:46993262-46993281 None:intergenic 35.0%
! ATTCGCATTTCGCTTTGTTA+CGG - chr5.1:46991499-46991518 MS.gene053696:intron 35.0%
! GTTGTTGATGAAGAAGAAGA+CGG - chr5.1:46990635-46990654 MS.gene053696:CDS 35.0%
! GTTTTCATGAAAAACACTGC+CGG - chr5.1:46990929-46990948 MS.gene053696:intron 35.0%
! TGATGCTATGAAGATTGGTT+GGG - chr5.1:46991213-46991232 MS.gene053696:intron 35.0%
! TTTGGTTCAAAGATCTCTGA+GGG + chr5.1:46993311-46993330 None:intergenic 35.0%
!! AAAAAAAAGGTTTTCGGGAG+TGG + chr5.1:46991686-46991705 None:intergenic 35.0%
!! AAAAAAAGGTTTTCGGGAGT+GGG + chr5.1:46991685-46991704 None:intergenic 35.0%
!! TTCGTAAAAGTACCACAAAC+CGG - chr5.1:46991935-46991954 MS.gene053696:intron 35.0%
!!! ATGACATTTTTTCAGCCTCA+AGG - chr5.1:46991292-46991311 MS.gene053696:intron 35.0%
!!! GGCTTTTTTGTTGAGAGAAA+TGG + chr5.1:46993219-46993238 None:intergenic 35.0%
AAATTGCACCAATTAGCCCT+AGG + chr5.1:46991169-46991188 None:intergenic 40.0%
AACAATGATCAAGGCTCTCA+AGG + chr5.1:46992363-46992382 None:intergenic 40.0%
AACACGAAAAAACTCGTTGC+TGG - chr5.1:46990896-46990915 MS.gene053696:intron 40.0%
AAGGGTGAAGATTCCTCTAA+AGG - chr5.1:46993633-46993652 MS.gene053696:CDS 40.0%
AATTGCACCAATTAGCCCTA+GGG + chr5.1:46991168-46991187 None:intergenic 40.0%
ACAATGATCAAGGCTCTCAA+GGG + chr5.1:46992362-46992381 None:intergenic 40.0%
ACTTGGAGCAACAATGATCA+AGG + chr5.1:46992372-46992391 None:intergenic 40.0%
AGAGAAAGAAAAATCTCCGG+TGG + chr5.1:46991815-46991834 None:intergenic 40.0%
AGCCCCAATTCAACAATGTT+TGG + chr5.1:46993329-46993348 None:intergenic 40.0%
AGGACGGTAACCATCAATAA+CGG + chr5.1:46990504-46990523 None:intergenic 40.0%
AGTTCTTGTCCTACAGTTAC+CGG + chr5.1:46990608-46990627 None:intergenic 40.0%
CAAACAATCGCCGTTATTGA+TGG - chr5.1:46990491-46990510 MS.gene053696:CDS 40.0%
CAAGTGTGTGAAGATCAAAG+AGG + chr5.1:46991866-46991885 None:intergenic 40.0%
CAATCATATGAGGAGCAAGA+AGG + chr5.1:46992821-46992840 None:intergenic 40.0%
CCCATGTAATTCATGGAGAT+TGG + chr5.1:46991899-46991918 None:intergenic 40.0%
GAAAGTGATGTCCATGATTC+TGG - chr5.1:46993606-46993625 MS.gene053696:CDS 40.0%
GAGTCCAACCAGTAGTAAAA+AGG + chr5.1:46990868-46990887 None:intergenic 40.0%
GCCAATCTCCATGAATTACA+TGG - chr5.1:46991895-46991914 MS.gene053696:intron 40.0%
GTGGATGATGCTATGAAGAT+TGG - chr5.1:46991208-46991227 MS.gene053696:intron 40.0%
TCCGTCATGAACATCAGTAA+CGG + chr5.1:46990801-46990820 None:intergenic 40.0%
TCCGTTACTGATGTTCATGA+CGG - chr5.1:46990797-46990816 MS.gene053696:CDS 40.0%
TCTTCTTCATCAACAACCTC+GGG + chr5.1:46990632-46990651 None:intergenic 40.0%
TGATCTTCACACACTTGTCT+CGG - chr5.1:46991869-46991888 MS.gene053696:intron 40.0%
TTCCTTTCCCTGTATCATCA+CGG - chr5.1:46990523-46990542 MS.gene053696:CDS 40.0%
TTTAGAGGAATCTTCACCCT+TGG + chr5.1:46993634-46993653 None:intergenic 40.0%
! AAATTCTCCTTTTCCGTCTC+AGG - chr5.1:46990769-46990788 MS.gene053696:CDS 40.0%
! AACACAAAAGGAGTTGGTTC+TGG - chr5.1:46993546-46993565 MS.gene053696:CDS 40.0%
! AAGTTTGCTCATGTCTATCG+AGG - chr5.1:46990827-46990846 MS.gene053696:CDS 40.0%
! AGAGAATGGTTTGGAGGTTA+AGG - chr5.1:46991036-46991055 MS.gene053696:intron 40.0%
! AGATTTTGAATTTCACGCGG+CGG + chr5.1:46990367-46990386 None:intergenic 40.0%
! AGTTTGCTCATGTCTATCGA+GGG - chr5.1:46990828-46990847 MS.gene053696:CDS 40.0%
! CAGCAAACTTCATGTTTCTG+CGG + chr5.1:46990973-46990992 None:intergenic 40.0%
! GACACCTTTTTACTACTGGT+TGG - chr5.1:46990861-46990880 MS.gene053696:intron 40.0%
! GAGAATGGTTTGGAGGTTAA+GGG - chr5.1:46991037-46991056 MS.gene053696:intron 40.0%
! GCCAGATTTTGAATTTCACG+CGG + chr5.1:46990370-46990389 None:intergenic 40.0%
! GGGTTAAGTTATAGGAGTGT+AGG - chr5.1:46992440-46992459 MS.gene053696:intron 40.0%
! GTAGAATCCTGAACTAGTCT+AGG + chr5.1:46993240-46993259 None:intergenic 40.0%
! GTTTGGTTCAAAGATCTCTG+AGG + chr5.1:46993312-46993331 None:intergenic 40.0%
! GTTTTCAGCAAGGAATGTGT+TGG + chr5.1:46992124-46992143 None:intergenic 40.0%
! TTCGCATTTCGCTTTGTTAC+GGG - chr5.1:46991500-46991519 MS.gene053696:intron 40.0%
!! ATAGCTTTGTTCTGCCCTAT+CGG - chr5.1:46992470-46992489 MS.gene053696:intron 40.0%
!! CACTTTTGCTGCTTCTATTG+CGG + chr5.1:46992272-46992291 None:intergenic 40.0%
!! GTTGTGATTGTGAGCATTGA+AGG + chr5.1:46992206-46992225 None:intergenic 40.0%
!! TTGATACTTGTACCGGTTTG+TGG + chr5.1:46991950-46991969 None:intergenic 40.0%
!!! CTTCAGGTGCTCTTCTTTTT+TGG - chr5.1:46991355-46991374 MS.gene053696:intron 40.0%
AAAATCTGGCAGACATGTGC+CGG - chr5.1:46990380-46990399 MS.gene053696:CDS 45.0%
AAGGAGGAGGGTAAAGAGAA+TGG - chr5.1:46991022-46991041 MS.gene053696:intron 45.0%
AATTCGATGCATGCATACGG+AGG - chr5.1:46991546-46991565 MS.gene053696:intron 45.0%
ACTGCCGGAGATATAGTTGT+TGG - chr5.1:46990944-46990963 MS.gene053696:intron 45.0%
AGAAATGGTAGATGCACTGC+AGG + chr5.1:46993204-46993223 None:intergenic 45.0%
ATCAAAACCGCGTCAATCCT+TGG - chr5.1:46993154-46993173 MS.gene053696:CDS 45.0%
ATGTGTCCAGTGTCCCTAAT+TGG - chr5.1:46991326-46991345 MS.gene053696:intron 45.0%
ATTGTTTGAGCGTTAGGGGA+AGG + chr5.1:46990479-46990498 None:intergenic 45.0%
CAATTTGGCGAAGACCTCAT+CGG + chr5.1:46990576-46990595 None:intergenic 45.0%
CATGTTCCAAGTGACCTTGT+GGG + chr5.1:46990451-46990470 None:intergenic 45.0%
CGCAATAGAAGCAGCAAAAG+TGG - chr5.1:46992270-46992289 MS.gene053696:intron 45.0%
GCCGCGTGAAATTCAAAATC+TGG - chr5.1:46990366-46990385 MS.gene053696:CDS 45.0%
GGAAGATCAAGTCAGCACAA+AGG + chr5.1:46990736-46990755 None:intergenic 45.0%
GGAGGGTAAAGAGAATGGTT+TGG - chr5.1:46991027-46991046 MS.gene053696:intron 45.0%
GGAGTTGGCGGAAAAGAATA+TGG - chr5.1:46991123-46991142 MS.gene053696:intron 45.0%
GTCCATGATTCTGGTACCAA+GGG - chr5.1:46993615-46993634 MS.gene053696:CDS 45.0%
GTGATGATACAGGGAAAGGA+AGG + chr5.1:46990524-46990543 None:intergenic 45.0%
TACCGGACTGAGTAGCAATT+TGG + chr5.1:46990591-46990610 None:intergenic 45.0%
TCTGATTCTAATAACGGCGG+TGG - chr5.1:46990695-46990714 MS.gene053696:CDS 45.0%
TGATACAGGGAAAGGAAGGA+CGG + chr5.1:46990520-46990539 None:intergenic 45.0%
TGTCCATGATTCTGGTACCA+AGG - chr5.1:46993614-46993633 MS.gene053696:CDS 45.0%
TTACTTCCCACAAGGTCACT+TGG - chr5.1:46990442-46990461 MS.gene053696:CDS 45.0%
TTGTTTGAGCGTTAGGGGAA+GGG + chr5.1:46990478-46990497 None:intergenic 45.0%
! AAGGGAGATGGTTTTTCTCG+AGG - chr5.1:46991055-46991074 MS.gene053696:intron 45.0%
! AGAAGCTTCAGGGTGTCTTA+TGG - chr5.1:46992902-46992921 MS.gene053696:CDS 45.0%
! ATGAAGATTGGTTGGGCATC+TGG - chr5.1:46991220-46991239 MS.gene053696:intron 45.0%
! CTCTGAGGGAACTCTATCAT+AGG + chr5.1:46993297-46993316 None:intergenic 45.0%
! CTGATGTTCATGACGGAGTT+TGG - chr5.1:46990804-46990823 MS.gene053696:CDS 45.0%
! GATTTTGAATTTCACGCGGC+GGG + chr5.1:46990366-46990385 None:intergenic 45.0%
! GGGTAAAGAGAATGGTTTGG+AGG - chr5.1:46991030-46991049 MS.gene053696:intron 45.0%
! TATTTTCCAGCAGGCATGCA+GGG - chr5.1:46993348-46993367 MS.gene053696:CDS 45.0%
! TTCGCTTTGTTACGGGTTTG+CGG - chr5.1:46991507-46991526 MS.gene053696:intron 45.0%
!! AATTGGGGCTATTTTCCAGC+AGG - chr5.1:46993339-46993358 MS.gene053696:CDS 45.0%
!! ATGTGTTGGTTGAGCTGAGA+TGG + chr5.1:46992110-46992129 None:intergenic 45.0%
!! AATTTAGTATATAAATATTA+GGG - chr5.1:46992420-46992439 MS.gene053696:intron 5.0%
!! TAATATTACAAAAAATTAAA+AGG - chr5.1:46991629-46991648 MS.gene053696:intron 5.0%
AACCGAGAAGGCGGTTATTG+AGG - chr5.1:46991096-46991115 MS.gene053696:intron 50.0%
AAGGGGAATGCTAACCGAGA+AGG - chr5.1:46991084-46991103 MS.gene053696:intron 50.0%
ACCTGAAGCATGCGCCAATT+AGG + chr5.1:46991343-46991362 None:intergenic 50.0%
ACGGCGATTGTTTGAGCGTT+AGG + chr5.1:46990485-46990504 None:intergenic 50.0%
AGCTGAGATGGTCAGAGGAT+TGG + chr5.1:46992098-46992117 None:intergenic 50.0%
AGGATCGGCTAGGAGATCAA+CGG + chr5.1:46990549-46990568 None:intergenic 50.0%
ATCAACGGCCGTGATGATAC+AGG + chr5.1:46990534-46990553 None:intergenic 50.0%
ATCAAGTCAGCACAAAGGCG+AGG + chr5.1:46990731-46990750 None:intergenic 50.0%
CAACTTGCCAAGGATTGACG+CGG + chr5.1:46993164-46993183 None:intergenic 50.0%
CACCCTTGGTACCAGAATCA+TGG + chr5.1:46993620-46993639 None:intergenic 50.0%
CCTGAAGCATGCGCCAATTA+GGG + chr5.1:46991342-46991361 None:intergenic 50.0%
CGCCAAATTGCTACTCAGTC+CGG - chr5.1:46990586-46990605 MS.gene053696:CDS 50.0%
CGGCGATTGTTTGAGCGTTA+GGG + chr5.1:46990484-46990503 None:intergenic 50.0%
CTACTCAGTCCGGTAACTGT+AGG - chr5.1:46990596-46990615 MS.gene053696:CDS 50.0%
CTTCACACACTTGTCTCGGT+TGG - chr5.1:46991873-46991892 MS.gene053696:intron 50.0%
GAGAGTTCCTGAGACGGAAA+AGG + chr5.1:46990779-46990798 None:intergenic 50.0%
GCATGTTCCAAGTGACCTTG+TGG + chr5.1:46990452-46990471 None:intergenic 50.0%
GGCGACAAGTCGTTAGATGT+GGG + chr5.1:46993468-46993487 None:intergenic 50.0%
GGCGATTGTTTGAGCGTTAG+GGG + chr5.1:46990483-46990502 None:intergenic 50.0%
GGTTATTGAGGCAGTGGAGT+TGG - chr5.1:46991108-46991127 MS.gene053696:intron 50.0%
GGTTTGGAGGTTAAGGGAGA+TGG - chr5.1:46991043-46991062 MS.gene053696:intron 50.0%
GTAGATGCACTGCAGGATTG+CGG + chr5.1:46993197-46993216 None:intergenic 50.0%
TAGATGCACTGCAGGATTGC+GGG + chr5.1:46993196-46993215 None:intergenic 50.0%
TATTGAGGCAGTGGAGTTGG+CGG - chr5.1:46991111-46991130 MS.gene053696:intron 50.0%
TCAACGGCCGTGATGATACA+GGG + chr5.1:46990533-46990552 None:intergenic 50.0%
TCCATCGGTGAACGTTCCAA+AGG - chr5.1:46990403-46990422 MS.gene053696:CDS 50.0%
TGCCTCAATAACCGCCTTCT+CGG + chr5.1:46991101-46991120 None:intergenic 50.0%
TGGCGACAAGTCGTTAGATG+TGG + chr5.1:46993469-46993488 None:intergenic 50.0%
TGTAGGACAAGAACTTCCCG+AGG - chr5.1:46990613-46990632 MS.gene053696:CDS 50.0%
! CTATTTTCCAGCAGGCATGC+AGG - chr5.1:46993347-46993366 MS.gene053696:CDS 50.0%
! GAGATGGTTTTTCTCGAGGC+GGG - chr5.1:46991059-46991078 MS.gene053696:intron 50.0%
! GGAGATGGTTTTTCTCGAGG+CGG - chr5.1:46991058-46991077 MS.gene053696:intron 50.0%
! GTAACGGAGAGTTCCTGAGA+CGG + chr5.1:46990785-46990804 None:intergenic 50.0%
! TTTTTCTCGAGGCGGGAGAA+GGG - chr5.1:46991066-46991085 MS.gene053696:intron 50.0%
!! ACCTTTGGAACGTTCACCGA+TGG + chr5.1:46990407-46990426 None:intergenic 50.0%
CATGCGCCAATTAGGGACAC+TGG + chr5.1:46991335-46991354 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTC+AGG - chr5.1:46991339-46991358 MS.gene053696:intron 55.0%
GAAGGCGGTTATTGAGGCAG+TGG - chr5.1:46991102-46991121 MS.gene053696:intron 55.0%
GACCTCATCGGTGTGAGGAT+CGG + chr5.1:46990564-46990583 None:intergenic 55.0%
GGCCGTGATGATACAGGGAA+AGG + chr5.1:46990528-46990547 None:intergenic 55.0%
GGGAATGCTAACCGAGAAGG+CGG - chr5.1:46991087-46991106 MS.gene053696:intron 55.0%
GGTTGAGCTGAGATGGTCAG+AGG + chr5.1:46992103-46992122 None:intergenic 55.0%
TGGAACGTTCACCGATGGAC+CGG + chr5.1:46990402-46990421 None:intergenic 55.0%
! GTTTTTCTCGAGGCGGGAGA+AGG - chr5.1:46991065-46991084 MS.gene053696:intron 55.0%
! TGAGGAGCAAGAAGGGTGCA+TGG + chr5.1:46992813-46992832 None:intergenic 55.0%
! TTTTCTCGAGGCGGGAGAAG+GGG - chr5.1:46991067-46991086 MS.gene053696:intron 55.0%
AGCCGATCCTCACACCGATG+AGG - chr5.1:46990559-46990578 MS.gene053696:CDS 60.0%
CATCGGTGTGAGGATCGGCT+AGG + chr5.1:46990559-46990578 None:intergenic 60.0%
GCGAAGACCTCATCGGTGTG+AGG + chr5.1:46990569-46990588 None:intergenic 60.0%
TTGAATTTCACGCGGCGGGC+CGG + chr5.1:46990362-46990381 None:intergenic 60.0%
TTGAGCGTTAGGGGAAGGGC+AGG + chr5.1:46990474-46990493 None:intergenic 60.0%
! GCAGACATGTGCCGGTCCAT+CGG - chr5.1:46990388-46990407 MS.gene053696:CDS 60.0%
CAGCAGGCATGCAGGGAGTC+AGG - chr5.1:46993355-46993374 MS.gene053696:CDS 65.0%
CTGACTCCCTGCATGCCTGC+TGG + chr5.1:46993357-46993376 None:intergenic 65.0%
GACAGACATGGTGGCCGGTG+GGG + chr5.1:46990336-46990355 None:intergenic 65.0%
GGACAGACATGGTGGCCGGT+GGG + chr5.1:46990337-46990356 None:intergenic 65.0%
GGAGGGACAGACATGGTGGC+CGG + chr5.1:46990341-46990360 None:intergenic 65.0%
GGCCACCATGTCTGTCCCTC+CGG - chr5.1:46990340-46990359 MS.gene053696:CDS 65.0%
AATTTCACGCGGCGGGCCGG+AGG + chr5.1:46990359-46990378 None:intergenic 70.0%
ATTTCACGCGGCGGGCCGGA+GGG + chr5.1:46990358-46990377 None:intergenic 70.0%
GGCCGGAGGGACAGACATGG+TGG + chr5.1:46990345-46990364 None:intergenic 70.0%
GGGACAGACATGGTGGCCGG+TGG + chr5.1:46990338-46990357 None:intergenic 70.0%
GCGGGCCGGAGGGACAGACA+TGG + chr5.1:46990348-46990367 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 46990326 46993671 46990326 ID=MS.gene053696
chr5.1 mRNA 46990326 46993671 46990326 ID=MS.gene053696.t1;Parent=MS.gene053696
chr5.1 exon 46992637 46993671 46992637 ID=MS.gene053696.t1.exon1;Parent=MS.gene053696.t1
chr5.1 CDS 46992637 46993671 46992637 ID=cds.MS.gene053696.t1;Parent=MS.gene053696.t1
chr5.1 exon 46990326 46990874 46990326 ID=MS.gene053696.t1.exon2;Parent=MS.gene053696.t1
chr5.1 CDS 46990326 46990874 46990326 ID=cds.MS.gene053696.t1;Parent=MS.gene053696.t1
Gene Sequence

>MS.gene053696

ATGTCTTCCCCACCGGCCACCATGTCTGTCCCTCCGGCCCGCCGCGTGAAATTCAAAATCTGGCAGACATGTGCCGGTCCATCGGTGAACGTTCCAAAGGTACGTTCTAAAGTTTATTACTTCCCACAAGGTCACTTGGAACATGCCTGCCCTTCCCCTAACGCTCAAACAATCGCCGTTATTGATGGTTACCGTCCTTCCTTTCCCTGTATCATCACGGCCGTTGATCTCCTAGCCGATCCTCACACCGATGAGGTCTTCGCCAAATTGCTACTCAGTCCGGTAACTGTAGGACAAGAACTTCCCGAGGTTGTTGATGAAGAAGAAGACGGTGATAAAGTTGTTTCCTTTGTAAAGACTCTTACAAAATCTGATTCTAATAACGGCGGTGGATTCTCTGTACCTCGCCTTTGTGCTGACTTGATCTTCCCCAAGCTTGACTTAAATTCTCCTTTTCCGTCTCAGGAACTCTCCGTTACTGATGTTCATGACGGAGTTTGGAAGTTTGCTCATGTCTATCGAGGGAGACCCAAAAGACACCTTTTTACTACTGGTTGGACTCCGTTTGTGAACACGAAAAAACTCGTTGCTGGTGATTCTATTGTTTTCATGAAAAACACTGCCGGAGATATAGTTGTTGGAATCCGCAGAAACATGAAGTTTGCTGCAGCTGAAGCAAAAGCAGTGAATAATAAGAAGGAGGAGGGTAAAGAGAATGGTTTGGAGGTTAAGGGAGATGGTTTTTCTCGAGGCGGGAGAAGGGGAATGCTAACCGAGAAGGCGGTTATTGAGGCAGTGGAGTTGGCGGAAAAGAATATGGCATTTGAAGTTATATATTACCCTAGGGCTAATTGGTGCAATTTTGTAGTTGATTCAAATGTTGTGGATGATGCTATGAAGATTGGTTGGGCATCTGGAATGAGAGTGAAGCTTCCTTTGAAGATTGATGAATCTTCAAATTCGAAGATGACATTTTTTCAGCCTCAAGGAACAATCTCTAATGTGTCCAGTGTCCCTAATTGGCGCATGCTTCAGGTGCTCTTCTTTTTTGGTCTTATTGTAGTTTTATTTTATTTTTTGAATCTAGGGCTGATTATATTTGACTGCATAATGTTATAAATATTATTGTCGAAGAAGTTTAGGCATGATTCATTTAGACGTTGTAGTCACATAATTCGCATTTCGCTTTGTTACGGGTTTGCGGTACACGACATGAGAAGAATTCGATGCATGCATACGGAGGTATAAACCTTGCATCGATTCATAGTATTGTTACTACCTAATTAAGTATACTAAACTATCTTAATATTACAAAAAATTAAAAGGATTATATCGACAAGCTACAACAAATTAGTGCCCACTCCCGAAAACCTTTTTTTTACACTCACTTTTCTTTCACTTTTTTACTTTTGTATCAATGTTATTTTGCAAGATACGTTCTCTTTTTTAGAGCTTCAAAGAGGTTTTCCAACAAAATTTATTTGCTCCACCGGAGATTTTTCTTTCTCTTTATCTCTTATTCAACCTCTTAATTGGTCCTCTTTGATCTTCACACACTTGTCTCGGTTGGCCAATCTCCATGAATTACATGGGTATTAGTGCAAATGAATTCGTAAAAGTACCACAAACCGGTACAAGTATCAATTCACAATTCGCAGCATGTCAAGAGAATTATGTGACTATGGTTGAAACTATAATCAAATGATGGTTCATGAAATAGATATCCAAAATTCTAATACCAAGCAATTATTAATGTAAAATACTAGTATCCAATCCTCTGACCATCTCAGCTCAACCAACACATTCCTTGCTGAAAACATTATTCAACGATTATGAGGCATGATTACCAAAATACAGTTAGGAAAAATCACAACAGACCTTCAATGCTCACAATCACAACAATAGCTAGCTGTATAATTGTAGCAGTGTCATCTTTTTTCTTTCCGCAATAGAAGCAGCAAAAGTGGTATATATGTTTAATCAAGAGGAATAGGGAAGTTATAGATAACTTGGAATGCAAAATAGAGTTACATCCCTTGAGAGCCTTGATCATTGTTGCTCCAAGTTTAAAAAATCATGTTTTATCCCTTGATCAATTTAGTATATAAATATTAGGGTTAAGTTATAGGAGTGTAGGCTACTGTATAGCTTTGTTCTGCCCTATCGGATAAGTTATAAATAAAATTCTTATATTATCTGACCAACTAAGTTAGTCGTTAATTTACTATTTTTTGGTGTTGTTATTATTTACCTTCAACACTAATAAAAGGGAAATATTAGAACATCAATTTATGTATAGTTGAGATAATTTTAAGAGAATACTTTTTTTTTTTTTTGGTACAAATTTTAAGAGAATACTTTAGTTTGATTAGAAATACATTTGAAGTATTTTATAGTTGATACCAAAATTAATCCATAATTATAGATCAACTGCAACACAATTCGTTCTTGCACTTTTATCGTTTGAGATCATTAGTTGCAAAACCATGCACCCTTCTTGCTCCTCATATGATTGAGTGATTGACGAAATGCGAGAATAAAAGAGTGACAAGTGTTTTCAGGCATATAAATAATGGAGAAGCTTCAGGGTGTCTTATGGAAAAGTATTAGATATTTAGGTTACCATATTTTTATAATAAAATGCATTTACTCTTATTCCTAAGTAGGATCTATATTTTACTCACATGTTATTAGTATTTTGTTAAGAAACCTTTTATGTGCATATACATACTCTAACTTATCGTATATTTTGAATGACTGATGTTGGATTCTTACCATTTTTATCTCTTTATCATAGGTTAACTGGGATGAACTTGAAATCTCGCAAAATCAAAACCGCGTCAATCCTTGGCAAGTTGAACTTATTTCCCGCAATCCTGCAGTGCATCTACCATTTCTCTCAACAAAAAAGCCTAGACTAGTTCAGGATTCTACCTTGTTTAGTGATAACAAGGGTGATCCCTTCATTCCTATGATAGAGTTCCCTCAGAGATCTTTGAACCAAACATTGTTGAATTGGGGCTATTTTCCAGCAGGCATGCAGGGAGTCAGGCATGATCATTTATCTCTATCAGGTTTTTCCAACTCTTTGAATGATAATAGTTATGGTTTCTTTGTGAACAATCTAAACATTGACAGTCCCACATCTAACGACTTGTCGCCAAATAACCTTAGTAGTTTGAACACATTCGGGACAGATTTTTTTGAGACTCACAACAACAACACAAAAGGAGTTGGTTCTGGTTCAATTAAGTTATTTGGTAAGATCATACAGACCGTTGAAAGTGATGTCCATGATTCTGGTACCAAGGGTGAAGATTCCTCTAAAGGATCCAATGAAATTTGA
Protein sequence

>MS.gene053696.t1

MSSPPATMSVPPARRVKFKIWQTCAGPSVNVPKVRSKVYYFPQGHLEHACPSPNAQTIAVIDGYRPSFPCIITAVDLLADPHTDEVFAKLLLSPVTVGQELPEVVDEEEDGDKVVSFVKTLTKSDSNNGGGFSVPRLCADLIFPKLDLNSPFPSQELSVTDVHDGVWKFAHVYRGRPKRHLFTTGWTPFVNTKKLVAGDSIVFMKNTAGDIVVGIRRNMKFAAAEAKAVNNKKEEGKENGLEVKGDGFSRGGRRGMLTEKAVIEAVELAEKNMAFEVIYYPRANWCNFVVDSNVVDDAMKIGWASGMRVKLPLKIDESSNSKMTFFQPQGTISNVSSVPNWRMLQVNWDELEISQNQNRVNPWQVELISRNPAVHLPFLSTKKPRLVQDSTLFSDNKGDPFIPMIEFPQRSLNQTLLNWGYFPAGMQGVRHDHLSLSGFSNSLNDNSYGFFVNNLNIDSPTSNDLSPNNLSSLNTFGTDFFETHNNNTKGVGSGSIKLFGKIIQTVESDVHDSGTKGEDSSKGSNEI