AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053715

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053715.t1 MTR_5g060780 66.398 372 104 8 1 357 9 374 6.74e-159 459
MS.gene053715.t1 MTR_5g061890 66.942 363 100 7 1 348 9 366 3.06e-158 457
MS.gene053715.t1 MTR_5g460920 66.129 372 105 8 1 357 9 374 6.01e-158 456
MS.gene053715.t1 MTR_5g061220 48.624 436 173 10 1 389 16 447 4.48e-125 377
MS.gene053715.t1 MTR_5g060630 51.961 408 132 8 1 345 9 415 1.48e-122 370
MS.gene053715.t1 MTR_5g460740 51.961 408 132 8 1 345 9 415 1.48e-122 370
MS.gene053715.t1 MTR_1g058210 52.500 360 146 9 1 341 9 362 4.10e-115 349
MS.gene053715.t1 MTR_2g024430 49.596 371 156 12 1 351 17 376 1.26e-103 322
MS.gene053715.t1 MTR_5g040880 50.301 332 141 9 1 316 8 331 2.28e-94 298
MS.gene053715.t1 MTR_5g082140 40.633 411 206 13 1 385 9 407 7.91e-86 273
MS.gene053715.t1 MTR_2g006270 44.413 358 169 10 4 341 13 360 1.91e-83 267
MS.gene053715.t1 MTR_2g006380 43.266 349 169 10 4 333 20 358 5.22e-79 256
MS.gene053715.t1 MTR_5g040740 48.101 316 136 9 1 297 9 315 2.84e-78 252
MS.gene053715.t1 MTR_5g060770 49.515 309 97 8 53 354 7 263 2.75e-71 232
MS.gene053715.t1 MTR_3g073420 34.592 503 244 18 1 433 16 503 1.54e-64 221
MS.gene053715.t1 MTR_5g062970 52.885 208 76 7 3 193 37 239 2.45e-61 202
MS.gene053715.t1 MTR_1g064430 33.187 455 209 15 1 373 24 465 8.54e-57 202
MS.gene053715.t1 MTR_1g064430 33.187 455 209 15 1 373 37 478 1.08e-56 202
MS.gene053715.t1 MTR_2g094570 34.375 384 180 12 1 322 8 381 1.17e-53 193
MS.gene053715.t1 MTR_2g094570 34.375 384 180 12 1 322 8 381 1.78e-53 192
MS.gene053715.t1 MTR_2g094570 34.375 384 180 12 1 322 64 437 2.99e-53 193
MS.gene053715.t1 MTR_5g460910 42.105 323 99 10 43 354 13 258 6.47e-52 181
MS.gene053715.t1 MTR_1g094960 33.508 382 175 12 1 317 16 383 1.96e-50 183
MS.gene053715.t1 MTR_4g058930 34.545 385 174 12 1 317 9 383 1.72e-48 179
MS.gene053715.t1 MTR_4g058930 34.545 385 174 12 1 317 9 383 1.81e-48 179
MS.gene053715.t1 MTR_7g101280 29.437 462 220 15 1 373 28 472 3.99e-46 172
MS.gene053715.t1 MTR_0041s0110 29.437 462 220 15 1 373 28 472 3.99e-46 172
MS.gene053715.t1 MTR_2g005240 31.961 413 203 15 4 377 23 396 3.12e-42 161
MS.gene053715.t1 MTR_2g018690 33.884 363 182 17 1 320 20 367 7.57e-39 152
MS.gene053715.t1 MTR_8g100050 31.563 339 200 9 4 316 59 391 2.88e-38 150
MS.gene053715.t1 MTR_8g100050 31.563 339 200 9 4 316 59 391 3.42e-38 150
MS.gene053715.t1 MTR_8g079492 32.687 361 188 15 1 320 20 366 3.77e-38 150
MS.gene053715.t1 MTR_8g079492 32.687 361 188 15 1 320 20 366 4.40e-38 150
MS.gene053715.t1 MTR_7g101275 31.562 320 172 10 14 301 2 306 8.74e-38 144
MS.gene053715.t1 MTR_2g093740 32.425 367 206 13 4 335 56 415 4.01e-37 147
MS.gene053715.t1 MTR_1g024025 31.844 358 203 12 1 324 40 390 4.10e-37 147
MS.gene053715.t1 MTR_2g093740 32.425 367 206 13 4 335 56 415 4.31e-37 146
MS.gene053715.t1 MTR_2g093740 32.425 367 206 13 4 335 56 415 5.96e-37 146
MS.gene053715.t1 MTR_5g076270 32.240 366 184 16 1 320 21 368 7.39e-37 146
MS.gene053715.t1 MTR_2g043250 32.370 346 194 15 1 316 20 355 1.53e-36 145
MS.gene053715.t1 MTR_2g014770 30.337 356 202 13 4 325 53 396 1.64e-36 144
MS.gene053715.t1 MTR_4g021580 30.245 367 211 14 2 335 17 371 2.43e-36 143
MS.gene053715.t1 MTR_2g014770 30.337 356 202 13 4 325 53 396 2.98e-36 144
MS.gene053715.t1 MTR_4g021580 30.245 367 211 14 2 335 17 371 3.03e-36 143
MS.gene053715.t1 MTR_4g088210 31.034 377 208 15 4 342 38 400 5.71e-36 143
MS.gene053715.t1 MTR_4g088210 31.034 377 208 15 4 342 38 400 9.39e-36 142
MS.gene053715.t1 MTR_3g064050 31.233 365 189 15 1 320 23 370 1.53e-35 142
MS.gene053715.t1 MTR_2g093740 31.316 380 206 13 4 335 56 428 3.30e-35 141
MS.gene053715.t1 MTR_2g093740 31.316 380 206 13 4 335 56 428 3.47e-35 140
MS.gene053715.t1 MTR_2g093740 31.316 380 206 13 4 335 56 428 4.40e-35 140
MS.gene053715.t1 MTR_8g027440 31.322 348 214 12 4 331 29 371 6.93e-35 139
MS.gene053715.t1 MTR_7g062540 28.997 369 210 14 4 336 14 366 1.23e-34 139
MS.gene053715.t1 MTR_4g124900 31.897 348 193 13 1 316 20 355 1.91e-34 139
MS.gene053715.t1 MTR_4g124900 31.897 348 193 13 1 316 20 355 1.92e-34 139
MS.gene053715.t1 MTR_5g062960 42.623 183 88 5 211 390 1 169 1.63e-32 124
MS.gene053715.t1 MTR_0041s0120 30.330 333 171 12 14 301 2 318 4.13e-31 125
MS.gene053715.t1 MTR_4g060460 29.570 372 214 12 4 338 54 414 1.04e-30 127
MS.gene053715.t1 MTR_4g060460 29.570 372 214 12 4 338 54 414 1.10e-30 127
MS.gene053715.t1 MTR_8g101360 31.161 353 191 11 1 316 28 365 4.15e-30 126
MS.gene053715.t1 MTR_8g079492 31.081 296 155 13 62 320 1 284 2.31e-24 108
MS.gene053715.t1 MTR_2g093740 31.200 250 152 7 104 335 38 285 3.09e-21 98.2
MS.gene053715.t1 MTR_2g093740 31.200 250 152 7 104 335 38 285 3.78e-21 97.8
MS.gene053715.t1 MTR_2g093740 29.658 263 152 7 104 335 38 298 1.55e-19 92.8
MS.gene053715.t1 MTR_2g093740 29.658 263 152 7 104 335 38 298 1.74e-19 92.8
MS.gene053715.t1 MTR_8g446900 27.397 292 169 12 53 316 12 288 1.24e-18 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053715.t1 AT1G77850 33.484 442 214 14 1 373 16 446 1.19e-65 224
MS.gene053715.t1 AT1G77850 33.484 442 214 14 1 373 16 446 1.44e-65 224
MS.gene053715.t1 AT4G30080 32.688 465 217 16 1 386 16 463 3.20e-52 189
MS.gene053715.t1 AT3G61830 34.190 389 207 16 3 362 24 392 5.01e-46 171
MS.gene053715.t1 AT2G28350 32.911 395 168 15 1 317 7 382 5.38e-45 169
MS.gene053715.t1 AT2G28350 32.911 395 168 15 1 317 7 382 5.86e-45 169
MS.gene053715.t1 AT2G46530 33.149 362 202 14 3 335 20 370 1.26e-43 164
MS.gene053715.t1 AT2G46530 33.149 362 202 14 3 335 41 391 1.40e-43 164
MS.gene053715.t1 AT5G37020 31.967 366 185 17 1 320 19 366 1.82e-40 157
MS.gene053715.t1 AT5G37020 31.967 366 185 17 1 320 19 366 3.06e-40 156
MS.gene053715.t1 AT1G59750 33.138 341 191 11 4 316 22 353 2.16e-39 152
MS.gene053715.t1 AT1G59750 33.138 341 191 11 4 316 22 353 2.35e-39 152
MS.gene053715.t1 AT1G59750 33.138 341 191 11 4 316 22 353 2.45e-39 152
MS.gene053715.t1 AT1G59750 33.138 341 191 11 4 316 22 353 2.45e-39 152
MS.gene053715.t1 AT2G33860 32.796 372 196 16 4 338 54 408 8.40e-39 150
MS.gene053715.t1 AT1G30330 32.500 360 191 14 1 320 22 369 1.75e-38 151
MS.gene053715.t1 AT1G30330 32.500 360 191 14 1 320 22 369 1.75e-38 151
MS.gene053715.t1 AT1G30330 32.500 360 191 14 1 320 20 367 1.79e-38 151
MS.gene053715.t1 AT1G19850 32.044 362 196 13 1 323 51 401 2.53e-37 147
MS.gene053715.t1 AT1G19850 32.044 362 196 13 1 323 40 390 2.61e-37 147
MS.gene053715.t1 AT5G62000 30.904 343 197 10 4 316 61 393 7.50e-37 146
MS.gene053715.t1 AT5G62000 30.904 343 197 10 4 316 61 393 7.50e-37 146
MS.gene053715.t1 AT5G62000 30.904 343 197 10 4 316 61 393 7.50e-37 146
MS.gene053715.t1 AT5G62000 30.904 343 197 10 4 316 61 393 7.50e-37 146
MS.gene053715.t1 AT5G62000 30.904 343 197 10 4 316 61 393 1.07e-36 145
MS.gene053715.t1 AT5G60450 31.016 374 213 13 3 343 40 401 1.77e-35 142
MS.gene053715.t1 AT5G60450 31.016 374 213 13 3 343 64 425 2.01e-35 142
MS.gene053715.t1 AT1G34390 31.778 343 191 12 1 316 24 350 2.91e-34 137
MS.gene053715.t1 AT1G35520 32.754 345 187 14 1 316 24 352 7.13e-34 136
MS.gene053715.t1 AT4G23980 29.714 350 194 13 4 318 12 344 7.63e-34 136
MS.gene053715.t1 AT4G23980 29.714 350 194 13 4 318 12 344 8.97e-34 136
MS.gene053715.t1 AT5G20730 30.595 353 192 12 1 316 21 357 1.40e-33 137
MS.gene053715.t1 AT5G20730 30.595 353 192 12 1 316 21 357 1.54e-33 136
MS.gene053715.t1 AT1G34410 32.174 345 189 14 1 316 24 352 1.75e-33 135
MS.gene053715.t1 AT5G20730 30.682 352 192 12 1 316 21 356 2.23e-33 136
MS.gene053715.t1 AT1G35540 31.085 341 198 12 1 316 24 352 2.28e-33 134
MS.gene053715.t1 AT1G19220 31.594 345 198 14 1 316 20 355 3.56e-33 135
MS.gene053715.t1 AT1G34310 33.041 342 184 14 4 316 27 352 3.60e-33 134
MS.gene053715.t1 AT1G35240 33.043 345 186 15 1 316 24 352 4.16e-33 134
MS.gene053715.t1 AT3G61830 33.238 349 188 15 39 362 13 341 2.12e-32 131
MS.gene053715.t1 AT1G34170 28.571 406 237 17 1 369 22 411 2.25e-30 125
MS.gene053715.t1 AT1G34170 27.778 396 227 15 1 369 22 385 9.65e-25 107
MS.gene053715.t1 AT1G34170 27.778 396 227 15 1 369 22 385 1.63e-24 107
MS.gene053715.t1 AT2G46530 32.549 255 143 11 103 335 36 283 1.10e-23 105
MS.gene053715.t1 AT2G33860 32.061 262 139 12 104 338 2 251 6.80e-23 102
MS.gene053715.t1 AT1G43950 34.135 208 111 7 1 191 24 222 3.56e-22 95.5
MS.gene053715.t1 AT5G37020 30.714 280 144 14 77 320 6 271 5.65e-22 100

Find 153 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTTATCATAGGTTAAA+TGG 0.116737 5.1:-46574878 MS.gene053715:intron
TGTTTCCATCTTCGTCTTCT+TGG 0.202132 5.1:+46575953 None:intergenic
ATAGTTAAATTTGAAGATTC+AGG 0.238302 5.1:+46575436 None:intergenic
GGTGGTTTCTGCGGGGTATT+AGG 0.250113 5.1:+46574849 None:intergenic
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.1:+46576180 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.1:-46575378 MS.gene053715:CDS
CCCTAATTGGCGCATGCTTA+TGG 0.282778 5.1:-46575365 MS.gene053715:intron
TCTTGAGACTTCTGCAACTC+TGG 0.293011 5.1:+46575844 None:intergenic
CAGTTCTACAGGTGCGGACT+TGG 0.294646 5.1:-46574361 MS.gene053715:CDS
ATGGTGATTGGGGCAGCATT+AGG 0.296457 5.1:+46576117 None:intergenic
TCACTTGGTGCTTGGCTCAT+TGG 0.308376 5.1:+46574119 None:intergenic
TGGTGATTGGGGCAGCATTA+GGG 0.311934 5.1:+46576118 None:intergenic
ACCATAAGCATGCGCCAATT+AGG 0.314871 5.1:+46575364 None:intergenic
CTACTTCCCACAAGGTCATT+TGG 0.318755 5.1:-46576157 MS.gene053715:CDS
CGCAGAGTCGACTGATACTT+TGG 0.321532 5.1:-46574406 MS.gene053715:CDS
ACATTTCCAACCAATCTTCA+TGG 0.335775 5.1:+46575489 None:intergenic
CAGTTCTAAAGGTGCGGACT+TGG 0.342634 5.1:-46574223 MS.gene053715:CDS
CAGTTCTAAAGGTGCGGACT+TGG 0.342634 5.1:-46574292 MS.gene053715:CDS
CTCTTTATCATAGGTTAAAT+GGG 0.347475 5.1:-46574877 MS.gene053715:intron
TCTTCAAATTTAACTATAAG+AGG 0.347983 5.1:-46575430 MS.gene053715:CDS
CCAAGCTTTAGTCTTGTATC+AGG 0.348766 5.1:+46574453 None:intergenic
TGACCAGGGGTGGTTTCTGC+GGG 0.350158 5.1:+46574841 None:intergenic
CTTGGTCAGTTACGGGAGTT+AGG 0.352495 5.1:+46576001 None:intergenic
AGCGCATTTCTGCCATAGTT+TGG 0.354882 5.1:+46576221 None:intergenic
GATGAAAACAACGGAATCTT+CGG 0.357948 5.1:+46575714 None:intergenic
TTCTCACCTCTTTCAGCTGC+AGG 0.358959 5.1:+46575640 None:intergenic
CCAACCTTTAGCCTTGTATC+AGG 0.361804 5.1:+46574522 None:intergenic
GCCCCAATCACCATTCTTGA+TGG 0.362674 5.1:-46576108 MS.gene053715:CDS
GAGGGCAAAGCTTCGTTTGA+TGG 0.363032 5.1:-46575461 MS.gene053715:CDS
GTCTCTGTCACTGACCTTCC+AGG 0.363192 5.1:-46575820 MS.gene053715:CDS
AGTGGTACTTTGCTTGATAC+AGG 0.364586 5.1:-46574603 MS.gene053715:CDS
GAAGAACTCTGAGAAAGTTT+AGG 0.368472 5.1:+46574750 None:intergenic
CCATAAGCATGCGCCAATTA+GGG 0.368474 5.1:+46575365 None:intergenic
ATTAACTGAGAGTGCGGTCT+TGG 0.368633 5.1:-46575617 MS.gene053715:CDS
CCTCCTCCTCCTGTGGTTCA+TGG 0.376894 5.1:-46575976 MS.gene053715:CDS
CCACAGGAGGAGGAGGTTCT+TGG 0.381303 5.1:+46575983 None:intergenic
AGTGATACTTTGGCTGATAC+AGG 0.384060 5.1:-46574258 MS.gene053715:CDS
AGTGATACTTTGGCTGATAC+AGG 0.384060 5.1:-46574327 MS.gene053715:CDS
ATGAAAACAACGGAATCTTC+GGG 0.384466 5.1:+46575715 None:intergenic
AGGAGGTTCTTGGTCAGTTA+CGG 0.395498 5.1:+46575993 None:intergenic
ACACAAACTGATACTAAATC+TGG 0.395790 5.1:-46575904 MS.gene053715:CDS
AACCATCAAGAATGGTGATT+GGG 0.402186 5.1:+46576106 None:intergenic
CAGTTCTAAAGGCGCTAACT+TGG 0.406148 5.1:-46574430 MS.gene053715:CDS
CTAAAGCTTGGCAGTTCTAC+AGG 0.408162 5.1:-46574372 MS.gene053715:CDS
GGAGGTTCTTGGTCAGTTAC+GGG 0.408872 5.1:+46575994 None:intergenic
GGTCTTGGGTGCAGTGGATT+TGG 0.411620 5.1:-46575602 MS.gene053715:CDS
TTTATCCCACAGATGGGAAT+TGG 0.411809 5.1:-46575549 MS.gene053715:CDS
TTGACCAGGGGTGGTTTCTG+CGG 0.423294 5.1:+46574840 None:intergenic
CTCCTATGGACTCCAAACTA+TGG 0.424679 5.1:-46576233 None:intergenic
GTGCTGAGTTGTTGCCTCAT+AGG 0.445159 5.1:-46574143 MS.gene053715:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.452082 5.1:-46574441 MS.gene053715:CDS
AACCACCCCTGGTCAATCCC+TGG 0.452665 5.1:-46574833 MS.gene053715:CDS
ATCTTTGGAATCTTGACGTC+GGG 0.453371 5.1:+46576195 None:intergenic
CTAAAGCTTGGCAGTTCTAA+AGG 0.454684 5.1:-46574165 MS.gene053715:CDS
CGCAGAGTCGAGTGATACTT+TGG 0.455775 5.1:-46574199 MS.gene053715:CDS
CGCAGAGTCGAGTGATACTT+TGG 0.455775 5.1:-46574268 MS.gene053715:CDS
CGCAGAGTCGAGTGATACTT+TGG 0.455775 5.1:-46574337 MS.gene053715:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.457123 5.1:-46574234 MS.gene053715:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.457123 5.1:-46574303 MS.gene053715:CDS
TACGGGAGTTAGGATCAGTT+TGG 0.462848 5.1:+46576011 None:intergenic
CATCTGGCATGCAAGGAGTC+AGG 0.465776 5.1:-46574656 MS.gene053715:CDS
GGTAATGGTGTCAGTGTACT+TGG 0.473148 5.1:+46574699 None:intergenic
TTAACTGAGAGTGCGGTCTT+GGG 0.478339 5.1:-46575616 MS.gene053715:CDS
TAACCATCAAGAATGGTGAT+TGG 0.479552 5.1:+46576105 None:intergenic
AACTTCCCTGCAGCTGAAAG+AGG 0.482726 5.1:-46575646 MS.gene053715:CDS
ATAAGCACTGGAGGTGGTAA+TGG 0.485821 5.1:+46574684 None:intergenic
GTATAGGTGTAGACTTTGCC+TGG 0.494583 5.1:+46575802 None:intergenic
GTAGATGATGCCATGAAGAT+TGG 0.497274 5.1:-46575499 MS.gene053715:CDS
CATGGCATCATCTACAACCT+TGG 0.499201 5.1:+46575507 None:intergenic
AGTTCTAAAGGTGCGGACTT+GGG 0.500873 5.1:-46574222 MS.gene053715:CDS
AGTTCTAAAGGTGCGGACTT+GGG 0.500873 5.1:-46574291 MS.gene053715:CDS
AGTTCTACAGGTGCGGACTT+GGG 0.504327 5.1:-46574360 MS.gene053715:CDS
GTTGTACTTTGCCTGATACA+AGG 0.504571 5.1:-46574533 MS.gene053715:CDS
CGGGTAAACTTCAACCTCCC+AGG 0.506933 5.1:+46574815 None:intergenic
AAGATACCTCACTTGGTGCT+TGG 0.507669 5.1:+46574111 None:intergenic
CTTGATACAGGGCTAAAGCT+TGG 0.508063 5.1:-46574591 MS.gene053715:CDS
TTTCTCAGAGTTCTTCTCCC+TGG 0.509292 5.1:-46574743 MS.gene053715:CDS
GGAGATGTTGACAGTGGAAG+AGG 0.510953 5.1:+46574785 None:intergenic
TTTGCCTGATACAAGGCTAA+AGG 0.515810 5.1:-46574526 MS.gene053715:CDS
GAAGGTGCTGACTTGTTCGC+AGG 0.520378 5.1:-46574630 MS.gene053715:CDS
TCTTCTTGGCCATGAACCAC+AGG 0.526193 5.1:+46575967 None:intergenic
GCTGATACAGTGCTAAAGCT+TGG 0.530738 5.1:-46574177 MS.gene053715:CDS
GCTGATACAGGGCTAAAGCT+TGG 0.532624 5.1:-46574246 MS.gene053715:CDS
GCTGATACAGGGCTAAAGCT+TGG 0.532624 5.1:-46574315 MS.gene053715:CDS
AGGTGTAGACTTTGCCTGGA+AGG 0.536557 5.1:+46575806 None:intergenic
GCTGATACAGAGCTAAAGCT+TGG 0.537434 5.1:-46574384 MS.gene053715:CDS
AAATATATGTCGGAATTCGC+AGG 0.543716 5.1:-46575675 MS.gene053715:CDS
GAGACGATGCATGGAATGGA+TGG 0.546365 5.1:+46576078 None:intergenic
AGTTCTAAAGGCGCTAACTT+GGG 0.546463 5.1:-46574429 MS.gene053715:CDS
CCATGAACCACAGGAGGAGG+AGG 0.550101 5.1:+46575976 None:intergenic
TGGCCATGAACCACAGGAGG+AGG 0.550434 5.1:+46575973 None:intergenic
CTTGGCTCATTGGACCTATG+AGG 0.550878 5.1:+46574129 None:intergenic
CCAAGAACCTCCTCCTCCTG+TGG 0.552145 5.1:-46575983 MS.gene053715:CDS
ATGAAGATTGGTTGGAAATG+TGG 0.555888 5.1:-46575487 MS.gene053715:CDS
ATGATGCCATGAAGATTGGT+TGG 0.558711 5.1:-46575495 MS.gene053715:CDS
CAACCTCCCAGGGATTGACC+AGG 0.559879 5.1:+46574826 None:intergenic
CATGGCCAAGAAGACGAAGA+TGG 0.560268 5.1:-46575958 MS.gene053715:CDS
CCTGATACAAGACTAAAGCT+TGG 0.563888 5.1:-46574453 MS.gene053715:CDS
GAGTGCGGTCTTGGGTGCAG+TGG 0.564030 5.1:-46575608 MS.gene053715:CDS
GGTGATTGGGGCAGCATTAG+GGG 0.566964 5.1:+46576119 None:intergenic
ATGCAAGGAGTCAGGCAAGA+AGG 0.567091 5.1:-46574648 MS.gene053715:CDS
AAACTATGGCAGAAATGCGC+TGG 0.567239 5.1:-46576219 MS.gene053715:CDS
ATTACACCAATTCCCATCTG+TGG 0.570163 5.1:+46575543 None:intergenic
TCCCAGGGATTGACCAGGGG+TGG 0.570982 5.1:+46574831 None:intergenic
AGTAGAGACGATGCATGGAA+TGG 0.572932 5.1:+46576074 None:intergenic
ACCACCCCTGGTCAATCCCT+GGG 0.574328 5.1:-46574832 MS.gene053715:CDS
GACTTGTTCGCAGGGTCGAG+TGG 0.575371 5.1:-46574621 MS.gene053715:CDS
AAACAGAGTAGACTAACTAA+AGG 0.575699 5.1:-46575772 MS.gene053715:CDS
TCTCACCTCTTTCAGCTGCA+GGG 0.578496 5.1:+46575641 None:intergenic
TGGTTGGAAATGTGGAATGA+GGG 0.580702 5.1:-46575479 MS.gene053715:CDS
CTGACTCCTTGCATGCCAGA+TGG 0.584361 5.1:+46574657 None:intergenic
ATCAAGTCTGCGCATTGACG+AGG 0.587901 5.1:+46575871 None:intergenic
CATGTTCCAAATGACCTTGT+GGG 0.588081 5.1:+46576151 None:intergenic
TGGACGGTAACCATCAAGAA+TGG 0.589526 5.1:+46576098 None:intergenic
GTGGTACTTTGCTTGATACA+GGG 0.591580 5.1:-46574602 MS.gene053715:CDS
CCTGATACAAGGCTAAAGGT+TGG 0.595695 5.1:-46574522 MS.gene053715:CDS
TTACACCAATTCCCATCTGT+GGG 0.595749 5.1:+46575544 None:intergenic
TTGGTTGGAAATGTGGAATG+AGG 0.597352 5.1:-46575480 MS.gene053715:CDS
GTGATACTTTGGCTGATACA+GGG 0.598282 5.1:-46574257 MS.gene053715:CDS
GTGATACTTTGGCTGATACA+GGG 0.598282 5.1:-46574326 MS.gene053715:CDS
TTGTGTGAGAGTCTTGACAT+AGG 0.600208 5.1:+46575921 None:intergenic
TGCCATAGTTTGGAGTCCAT+AGG 0.605278 5.1:+46576231 None:intergenic
GCCAATGAAGATACCTCACT+TGG 0.608165 5.1:+46574104 None:intergenic
GCAAAGACCTCGTCGGTGCG+AGG 0.609060 5.1:+46576033 None:intergenic
CGATGCATGGAATGGATGGA+CGG 0.620709 5.1:+46576082 None:intergenic
ACCATCAAGAATGGTGATTG+GGG 0.621569 5.1:+46576107 None:intergenic
CAGTGGAAGAGGAAGTTGAC+GGG 0.623769 5.1:+46574796 None:intergenic
GGGTAAACTTCAACCTCCCA+GGG 0.625766 5.1:+46574816 None:intergenic
TCAACAGTAGAGACGATGCA+TGG 0.626713 5.1:+46576069 None:intergenic
AGAGTAGACTAACTAAAGGA+TGG 0.627717 5.1:-46575768 MS.gene053715:CDS
TGTTGTGGAGATGTTGACAG+TGG 0.633588 5.1:+46574779 None:intergenic
GCATGTTCCAAATGACCTTG+TGG 0.633704 5.1:+46576150 None:intergenic
TACCCCGCAGAAACCACCCC+TGG 0.634765 5.1:-46574844 MS.gene053715:CDS
ACCTCCCAGGGATTGACCAG+GGG 0.638700 5.1:+46574828 None:intergenic
AACCTCCCAGGGATTGACCA+GGG 0.639624 5.1:+46574827 None:intergenic
AATCTTTGGAATCTTGACGT+CGG 0.641188 5.1:+46576194 None:intergenic
GACCAGGGGTGGTTTCTGCG+GGG 0.641988 5.1:+46574842 None:intergenic
AAGGTGCTGACTTGTTCGCA+GGG 0.642119 5.1:-46574629 MS.gene053715:CDS
ACAGTGGAAGAGGAAGTTGA+CGG 0.650244 5.1:+46574795 None:intergenic
TGTTGATCCTCGCACCGACG+AGG 0.652791 5.1:-46576040 MS.gene053715:CDS
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5.1:-46574229 MS.gene053715:CDS
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5.1:-46574298 MS.gene053715:CDS
AATGAGCCAAGCACCAAGTG+AGG 0.655123 5.1:-46574117 None:intergenic
GCTTGGCAGTTCTACAGGTG+CGG 0.659476 5.1:-46574367 MS.gene053715:CDS
GACTTGTTTGCAGAGTCGAG+TGG 0.663016 5.1:-46574483 MS.gene053715:CDS
CAGTTTGGCAAAGACCTCGT+CGG 0.663811 5.1:+46576026 None:intergenic
TCTGCCGGGAAAATATATGT+CGG 0.676335 5.1:-46575685 MS.gene053715:CDS
AAAGTTTACTACTTCCCACA+AGG 0.678948 5.1:-46576165 MS.gene053715:CDS
TCTTGGCCATGAACCACAGG+AGG 0.679438 5.1:+46575970 None:intergenic
TGAGAAATTAACTGAGAGTG+CGG 0.683957 5.1:-46575623 MS.gene053715:CDS
TGAAAACAACGGAATCTTCG+GGG 0.698750 5.1:+46575716 None:intergenic
ACCCCTGGTCAATCCCTGGG+AGG 0.702615 5.1:-46574829 MS.gene053715:CDS
GGATTTGGCTAACAAGAACG+TGG 0.730069 5.1:-46575587 MS.gene053715:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGATAAAGAGATAAAAAA+TGG + chr5.1:46575462-46575481 None:intergenic 15.0%
!!! ATTTTTTATCTCTTTATCAT+AGG - chr5.1:46575461-46575480 MS.gene053715:CDS 15.0%
!!! TAAATCAATCTTGTTTATTA+TGG + chr5.1:46575070-46575089 None:intergenic 15.0%
!! ATAGTTAAATTTGAAGATTC+AGG + chr5.1:46574914-46574933 None:intergenic 20.0%
!! TCTTCAAATTTAACTATAAG+AGG - chr5.1:46574917-46574936 MS.gene053715:intron 20.0%
! ACATTTGACACTTATTCTAT+AGG + chr5.1:46575191-46575210 None:intergenic 25.0%
! ACGTAATCTACTTACATTAA+CGG + chr5.1:46575297-46575316 None:intergenic 25.0%
! CATTTGACACTTATTCTATA+GGG + chr5.1:46575190-46575209 None:intergenic 25.0%
! CTCTTTATCATAGGTTAAAT+GGG - chr5.1:46575470-46575489 MS.gene053715:CDS 25.0%
! TCTCTTTATCATAGGTTAAA+TGG - chr5.1:46575469-46575488 MS.gene053715:CDS 25.0%
!! AGAGTTTTTGATGAAAACAA+CGG + chr5.1:46574645-46574664 None:intergenic 25.0%
!!! ATAAAACTTTGAGACTTAGT+AGG - chr5.1:46575131-46575150 MS.gene053715:intron 25.0%
!!! GCTTTCTTATTTTGTTCTAA+TGG - chr5.1:46575012-46575031 MS.gene053715:intron 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + chr5.1:46574170-46574189 None:intergenic 25.0%
AAACAGAGTAGACTAACTAA+AGG - chr5.1:46574575-46574594 MS.gene053715:CDS 30.0%
ACACAAACTGATACTAAATC+TGG - chr5.1:46574443-46574462 MS.gene053715:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5.1:46574969-46574988 MS.gene053715:intron 30.0%
CCAAAATAGAGATTCTACTT+AGG + chr5.1:46575345-46575364 None:intergenic 30.0%
GGAAATAATAATGTAGCAGA+TGG + chr5.1:46575253-46575272 None:intergenic 30.0%
TCATGAGATATCAAGAACAA+TGG + chr5.1:46575274-46575293 None:intergenic 30.0%
! AATTCCGACATATATTTTCC+CGG + chr5.1:46574669-46574688 None:intergenic 30.0%
! CCTAAGTAGAATCTCTATTT+TGG - chr5.1:46575342-46575361 MS.gene053715:intron 30.0%
! GAAGAATTTTGAATTTGCCA+GGG + chr5.1:46575624-46575643 None:intergenic 30.0%
! TAACTATAAGAGGTTTTCAG+CGG - chr5.1:46574927-46574946 MS.gene053715:intron 30.0%
! TGAAGAATTTTGAATTTGCC+AGG + chr5.1:46575625-46575644 None:intergenic 30.0%
!! AAGTTTAGGACTTTTTGTTG+TGG + chr5.1:46575586-46575605 None:intergenic 30.0%
AAAGTTTACTACTTCCCACA+AGG - chr5.1:46574182-46574201 MS.gene053715:CDS 35.0%
AAATATATGTCGGAATTCGC+AGG - chr5.1:46574672-46574691 MS.gene053715:CDS 35.0%
AACCATCAAGAATGGTGATT+GGG + chr5.1:46574244-46574263 None:intergenic 35.0%
ACATTTCCAACCAATCTTCA+TGG + chr5.1:46574861-46574880 None:intergenic 35.0%
AGAGTAGACTAACTAAAGGA+TGG - chr5.1:46574579-46574598 MS.gene053715:CDS 35.0%
ATGAAAACAACGGAATCTTC+GGG + chr5.1:46574635-46574654 None:intergenic 35.0%
GATGAAAACAACGGAATCTT+CGG + chr5.1:46574636-46574655 None:intergenic 35.0%
TAACCATCAAGAATGGTGAT+TGG + chr5.1:46574245-46574264 None:intergenic 35.0%
TGAGAAATTAACTGAGAGTG+CGG - chr5.1:46574724-46574743 MS.gene053715:CDS 35.0%
! AACTATAAGAGGTTTTCAGC+GGG - chr5.1:46574928-46574947 MS.gene053715:intron 35.0%
! ATGAAGATTGGTTGGAAATG+TGG - chr5.1:46574860-46574879 MS.gene053715:CDS 35.0%
! GAAGAACTCTGAGAAAGTTT+AGG + chr5.1:46575600-46575619 None:intergenic 35.0%
! GAATTGGTGTAATTTTGTGG+TGG - chr5.1:46574814-46574833 MS.gene053715:CDS 35.0%
! GTTTTCATCAAAAACTCTGC+CGG - chr5.1:46574647-46574666 MS.gene053715:CDS 35.0%
! TGGGAATTGGTGTAATTTTG+TGG - chr5.1:46574811-46574830 MS.gene053715:CDS 35.0%
! TTGTGTTTTATCCCACAGAT+GGG - chr5.1:46574792-46574811 MS.gene053715:CDS 35.0%
! TTTTCATCAAAAACTCTGCC+GGG - chr5.1:46574648-46574667 MS.gene053715:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG + chr5.1:46574156-46574175 None:intergenic 35.0%
!! ACTCTGTTTTGAGTAGGTAT+AGG + chr5.1:46574564-46574583 None:intergenic 35.0%
!! GTTGCTACTGTTTTGAATGT+AGG - chr5.1:46575153-46575172 MS.gene053715:intron 35.0%
!! TAGTCTACTCTGTTTTGAGT+AGG + chr5.1:46574570-46574589 None:intergenic 35.0%
ACCATCAAGAATGGTGATTG+GGG + chr5.1:46574243-46574262 None:intergenic 40.0%
AGTGATACTTTGGCTGATAC+AGG - chr5.1:46576020-46576039 MS.gene053715:CDS 40.0%
AGTGGTACTTTGCTTGATAC+AGG - chr5.1:46575744-46575763 MS.gene053715:CDS 40.0%
ATGATGCCATGAAGATTGGT+TGG - chr5.1:46574852-46574871 MS.gene053715:CDS 40.0%
ATTACACCAATTCCCATCTG+TGG + chr5.1:46574807-46574826 None:intergenic 40.0%
CATGTTCCAAATGACCTTGT+GGG + chr5.1:46574199-46574218 None:intergenic 40.0%
CCAGATGGAAAAATAAGCAC+TGG + chr5.1:46575678-46575697 None:intergenic 40.0%
CCTGATACAAGACTAAAGCT+TGG - chr5.1:46575894-46575913 MS.gene053715:CDS 40.0%
GTAGATGATGCCATGAAGAT+TGG - chr5.1:46574848-46574867 MS.gene053715:CDS 40.0%
GTGATACTTTGGCTGATACA+GGG - chr5.1:46576021-46576040 MS.gene053715:CDS 40.0%
GTGGTACTTTGCTTGATACA+GGG - chr5.1:46575745-46575764 MS.gene053715:CDS 40.0%
GTTGTACTTTGCCTGATACA+AGG - chr5.1:46575814-46575833 MS.gene053715:CDS 40.0%
TCTGCCGGGAAAATATATGT+CGG - chr5.1:46574662-46574681 MS.gene053715:CDS 40.0%
TGAAAACAACGGAATCTTCG+GGG + chr5.1:46574634-46574653 None:intergenic 40.0%
TGGTTGGAAATGTGGAATGA+GGG - chr5.1:46574868-46574887 MS.gene053715:intron 40.0%
TGTTTCCATCTTCGTCTTCT+TGG + chr5.1:46574397-46574416 None:intergenic 40.0%
TTACACCAATTCCCATCTGT+GGG + chr5.1:46574806-46574825 None:intergenic 40.0%
TTGGTTGGAAATGTGGAATG+AGG - chr5.1:46574867-46574886 MS.gene053715:CDS 40.0%
TTGTGTGAGAGTCTTGACAT+AGG + chr5.1:46574429-46574448 None:intergenic 40.0%
TTTATCCCACAGATGGGAAT+TGG - chr5.1:46574798-46574817 MS.gene053715:CDS 40.0%
TTTGCCTGATACAAGGCTAA+AGG - chr5.1:46575821-46575840 MS.gene053715:CDS 40.0%
! ATTTTTCCATCTGGCATGCA+AGG - chr5.1:46575684-46575703 MS.gene053715:CDS 40.0%
! CTAAAGCTTGGCAGTTCTAA+AGG - chr5.1:46575768-46575787 MS.gene053715:CDS 40.0%
! GTTGTGTTTTATCCCACAGA+TGG - chr5.1:46574791-46574810 MS.gene053715:CDS 40.0%
!! AGTTCTAAAGGCGCTAACTT+GGG - chr5.1:46575918-46575937 MS.gene053715:CDS 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG + chr5.1:46574155-46574174 None:intergenic 40.0%
!! CCAAGCTTTAGTCTTGTATC+AGG + chr5.1:46575897-46575916 None:intergenic 40.0%
!! CCAGTGCTTATTTTTCCATC+TGG - chr5.1:46575675-46575694 MS.gene053715:CDS 40.0%
!! GATGGAAAAATAAGCACTGG+AGG + chr5.1:46575675-46575694 None:intergenic 40.0%
!!! TAATTTTGTGGTGGATGCCA+AGG - chr5.1:46574823-46574842 MS.gene053715:CDS 40.0%
AAACTATGGCAGAAATGCGC+TGG - chr5.1:46574128-46574147 MS.gene053715:CDS 45.0%
ACAGTGGAAGAGGAAGTTGA+CGG + chr5.1:46575555-46575574 None:intergenic 45.0%
ACCATAAGCATGCGCCAATT+AGG + chr5.1:46574986-46575005 None:intergenic 45.0%
AGCGCATTTCTGCCATAGTT+TGG + chr5.1:46574129-46574148 None:intergenic 45.0%
AGGAGGTTCTTGGTCAGTTA+CGG + chr5.1:46574357-46574376 None:intergenic 45.0%
AGTAGAGACGATGCATGGAA+TGG + chr5.1:46574276-46574295 None:intergenic 45.0%
CATGGCATCATCTACAACCT+TGG + chr5.1:46574843-46574862 None:intergenic 45.0%
CCATAAGCATGCGCCAATTA+GGG + chr5.1:46574985-46575004 None:intergenic 45.0%
CCTGATACAAGGCTAAAGGT+TGG - chr5.1:46575825-46575844 MS.gene053715:CDS 45.0%
CTAAAGCTTGGCAGTTCTAC+AGG - chr5.1:46575975-46575994 MS.gene053715:CDS 45.0%
CTACTTCCCACAAGGTCATT+TGG - chr5.1:46574190-46574209 MS.gene053715:CDS 45.0%
CTTGATACAGGGCTAAAGCT+TGG - chr5.1:46575756-46575775 MS.gene053715:CDS 45.0%
GCATGTTCCAAATGACCTTG+TGG + chr5.1:46574200-46574219 None:intergenic 45.0%
GCTGATACAGAGCTAAAGCT+TGG - chr5.1:46575963-46575982 MS.gene053715:CDS 45.0%
GCTGATACAGTGCTAAAGCT+TGG - chr5.1:46576170-46576189 MS.gene053715:CDS 45.0%
GGATTTGGCTAACAAGAACG+TGG - chr5.1:46574760-46574779 MS.gene053715:CDS 45.0%
GTATAGGTGTAGACTTTGCC+TGG + chr5.1:46574548-46574567 None:intergenic 45.0%
TACGGGAGTTAGGATCAGTT+TGG + chr5.1:46574339-46574358 None:intergenic 45.0%
TCAACAGTAGAGACGATGCA+TGG + chr5.1:46574281-46574300 None:intergenic 45.0%
TCTTGAGACTTCTGCAACTC+TGG + chr5.1:46574506-46574525 None:intergenic 45.0%
TGGACGGTAACCATCAAGAA+TGG + chr5.1:46574252-46574271 None:intergenic 45.0%
TGTTGTGGAGATGTTGACAG+TGG + chr5.1:46575571-46575590 None:intergenic 45.0%
TTTCTCAGAGTTCTTCTCCC+TGG - chr5.1:46575604-46575623 MS.gene053715:CDS 45.0%
! ATAAGAGGTTTTCAGCGGGA+GGG - chr5.1:46574932-46574951 MS.gene053715:intron 45.0%
! ATTAACTGAGAGTGCGGTCT+TGG - chr5.1:46574730-46574749 MS.gene053715:CDS 45.0%
! GGTAATGGTGTCAGTGTACT+TGG + chr5.1:46575651-46575670 None:intergenic 45.0%
! TATAAGAGGTTTTCAGCGGG+AGG - chr5.1:46574931-46574950 MS.gene053715:intron 45.0%
! TTAACTGAGAGTGCGGTCTT+GGG - chr5.1:46574731-46574750 MS.gene053715:CDS 45.0%
!! AGTTCTAAAGGTGCGGACTT+GGG - chr5.1:46576056-46576075 MS.gene053715:CDS 45.0%
!! ATAAGCACTGGAGGTGGTAA+TGG + chr5.1:46575666-46575685 None:intergenic 45.0%
!! CAGTTCTAAAGGCGCTAACT+TGG - chr5.1:46575917-46575936 MS.gene053715:CDS 45.0%
!! CCAACCTTTAGCCTTGTATC+AGG + chr5.1:46575828-46575847 None:intergenic 45.0%
!! GGAAAAATAAGCACTGGAGG+TGG + chr5.1:46575672-46575691 None:intergenic 45.0%
AACTTCCCTGCAGCTGAAAG+AGG - chr5.1:46574701-46574720 MS.gene053715:CDS 50.0%
AGGTGTAGACTTTGCCTGGA+AGG + chr5.1:46574544-46574563 None:intergenic 50.0%
AGTTCTACAGGTGCGGACTT+GGG - chr5.1:46575987-46576006 MS.gene053715:CDS 50.0%
ATGCAAGGAGTCAGGCAAGA+AGG - chr5.1:46575699-46575718 MS.gene053715:CDS 50.0%
ATGGTGATTGGGGCAGCATT+AGG + chr5.1:46574233-46574252 None:intergenic 50.0%
CAGTGGAAGAGGAAGTTGAC+GGG + chr5.1:46575554-46575573 None:intergenic 50.0%
CAGTTTGGCAAAGACCTCGT+CGG + chr5.1:46574324-46574343 None:intergenic 50.0%
CATGGCCAAGAAGACGAAGA+TGG - chr5.1:46574389-46574408 MS.gene053715:CDS 50.0%
CCCTAATTGGCGCATGCTTA+TGG - chr5.1:46574982-46575001 MS.gene053715:intron 50.0%
CGATGCATGGAATGGATGGA+CGG + chr5.1:46574268-46574287 None:intergenic 50.0%
CGCAGAGTCGACTGATACTT+TGG - chr5.1:46575941-46575960 MS.gene053715:CDS 50.0%
CGCAGAGTCGAGTGATACTT+TGG - chr5.1:46576010-46576029 MS.gene053715:CDS 50.0%
CTTGGCTCATTGGACCTATG+AGG + chr5.1:46576221-46576240 None:intergenic 50.0%
CTTGGTCAGTTACGGGAGTT+AGG + chr5.1:46574349-46574368 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + chr5.1:46574272-46574291 None:intergenic 50.0%
GAGGGCAAAGCTTCGTTTGA+TGG - chr5.1:46574886-46574905 MS.gene053715:intron 50.0%
GCCCCAATCACCATTCTTGA+TGG - chr5.1:46574239-46574258 MS.gene053715:CDS 50.0%
GCTGATACAGGGCTAAAGCT+TGG - chr5.1:46576032-46576051 MS.gene053715:CDS 50.0%
GGAGATGTTGACAGTGGAAG+AGG + chr5.1:46575565-46575584 None:intergenic 50.0%
GGAGGTTCTTGGTCAGTTAC+GGG + chr5.1:46574356-46574375 None:intergenic 50.0%
GGGTAAACTTCAACCTCCCA+GGG + chr5.1:46575534-46575553 None:intergenic 50.0%
GTGCTGAGTTGTTGCCTCAT+AGG - chr5.1:46576204-46576223 MS.gene053715:CDS 50.0%
TCTCACCTCTTTCAGCTGCA+GGG + chr5.1:46574709-46574728 None:intergenic 50.0%
TCTTCTTGGCCATGAACCAC+AGG + chr5.1:46574383-46574402 None:intergenic 50.0%
TGGTGATTGGGGCAGCATTA+GGG + chr5.1:46574232-46574251 None:intergenic 50.0%
TTCTCACCTCTTTCAGCTGC+AGG + chr5.1:46574710-46574729 None:intergenic 50.0%
! ATCAAGTCTGCGCATTGACG+AGG + chr5.1:46574479-46574498 None:intergenic 50.0%
! GACTTGTTTGCAGAGTCGAG+TGG - chr5.1:46575864-46575883 MS.gene053715:CDS 50.0%
! TAAGAGGTTTTCAGCGGGAG+GGG - chr5.1:46574933-46574952 MS.gene053715:intron 50.0%
!! AAGGTGCTGACTTGTTCGCA+GGG - chr5.1:46575718-46575737 MS.gene053715:CDS 50.0%
!! CAGTTCTAAAGGTGCGGACT+TGG - chr5.1:46576055-46576074 MS.gene053715:CDS 50.0%
!! GCTTGGCAGTTCTAAAGGTG+CGG - chr5.1:46576049-46576068 MS.gene053715:CDS 50.0%
!! TCACTTGGTGCTTGGCTCAT+TGG + chr5.1:46576231-46576250 None:intergenic 50.0%
AACCTCCCAGGGATTGACCA+GGG + chr5.1:46575523-46575542 None:intergenic 55.0%
CAGTTCTACAGGTGCGGACT+TGG - chr5.1:46575986-46576005 MS.gene053715:CDS 55.0%
CATCTGGCATGCAAGGAGTC+AGG - chr5.1:46575691-46575710 MS.gene053715:CDS 55.0%
CGGGTAAACTTCAACCTCCC+AGG + chr5.1:46575535-46575554 None:intergenic 55.0%
CTGACTCCTTGCATGCCAGA+TGG + chr5.1:46575693-46575712 None:intergenic 55.0%
GCTTGGCAGTTCTACAGGTG+CGG - chr5.1:46575980-46575999 MS.gene053715:CDS 55.0%
GGTGATTGGGGCAGCATTAG+GGG + chr5.1:46574231-46574250 None:intergenic 55.0%
GTCTCTGTCACTGACCTTCC+AGG - chr5.1:46574527-46574546 MS.gene053715:CDS 55.0%
TCTTGGCCATGAACCACAGG+AGG + chr5.1:46574380-46574399 None:intergenic 55.0%
TTGACCAGGGGTGGTTTCTG+CGG + chr5.1:46575510-46575529 None:intergenic 55.0%
! GGTCTTGGGTGCAGTGGATT+TGG - chr5.1:46574745-46574764 MS.gene053715:CDS 55.0%
! GGTGGTTTCTGCGGGGTATT+AGG + chr5.1:46575501-46575520 None:intergenic 55.0%
!! GAAGGTGCTGACTTGTTCGC+AGG - chr5.1:46575717-46575736 MS.gene053715:CDS 55.0%
AACCACCCCTGGTCAATCCC+TGG - chr5.1:46575514-46575533 MS.gene053715:CDS 60.0%
ACCACCCCTGGTCAATCCCT+GGG - chr5.1:46575515-46575534 MS.gene053715:CDS 60.0%
ACCTCCCAGGGATTGACCAG+GGG + chr5.1:46575522-46575541 None:intergenic 60.0%
CAACCTCCCAGGGATTGACC+AGG + chr5.1:46575524-46575543 None:intergenic 60.0%
CCAAGAACCTCCTCCTCCTG+TGG - chr5.1:46574364-46574383 MS.gene053715:CDS 60.0%
CCACAGGAGGAGGAGGTTCT+TGG + chr5.1:46574367-46574386 None:intergenic 60.0%
CCATGAACCACAGGAGGAGG+AGG + chr5.1:46574374-46574393 None:intergenic 60.0%
CCTCCTCCTCCTGTGGTTCA+TGG - chr5.1:46574371-46574390 MS.gene053715:CDS 60.0%
TGACCAGGGGTGGTTTCTGC+GGG + chr5.1:46575509-46575528 None:intergenic 60.0%
TGGCCATGAACCACAGGAGG+AGG + chr5.1:46574377-46574396 None:intergenic 60.0%
! GACTTGTTCGCAGGGTCGAG+TGG - chr5.1:46575726-46575745 MS.gene053715:CDS 60.0%
! TGTTGATCCTCGCACCGACG+AGG - chr5.1:46574307-46574326 MS.gene053715:CDS 60.0%
ACCCCTGGTCAATCCCTGGG+AGG - chr5.1:46575518-46575537 MS.gene053715:CDS 65.0%
GACCAGGGGTGGTTTCTGCG+GGG + chr5.1:46575508-46575527 None:intergenic 65.0%
GAGTGCGGTCTTGGGTGCAG+TGG - chr5.1:46574739-46574758 MS.gene053715:CDS 65.0%
GCAAAGACCTCGTCGGTGCG+AGG + chr5.1:46574317-46574336 None:intergenic 65.0%
TACCCCGCAGAAACCACCCC+TGG - chr5.1:46575503-46575522 MS.gene053715:CDS 65.0%
TCCCAGGGATTGACCAGGGG+TGG + chr5.1:46575519-46575538 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 46574119 46576250 46574119 ID=MS.gene053715
chr5.1 mRNA 46574119 46576250 46574119 ID=MS.gene053715.t1;Parent=MS.gene053715
chr5.1 exon 46575366 46576250 46575366 ID=MS.gene053715.t1.exon1;Parent=MS.gene053715.t1
chr5.1 CDS 46575366 46576250 46575366 ID=cds.MS.gene053715.t1;Parent=MS.gene053715.t1
chr5.1 exon 46574119 46574886 46574119 ID=MS.gene053715.t1.exon2;Parent=MS.gene053715.t1
chr5.1 CDS 46574119 46574886 46574119 ID=cds.MS.gene053715.t1;Parent=MS.gene053715.t1
Gene Sequence

>MS.gene053715

ATGGACTCCAAACTATGGCAGAAATGCGCTGGTCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACAAGGTCATTTGGAACATGCATGTTCTTCCCCTAATGCTGCCCCAATCACCATTCTTGATGGTTACCGTCCATCCATTCCATGCATCGTCTCTACTGTTGATCTCTTTGTTGATCCTCGCACCGACGAGGTCTTTGCCAAACTGATCCTAACTCCCGTAACTGACCAAGAACCTCCTCCTCCTGTGGTTCATGGCCAAGAAGACGAAGATGGAAACAATCTTGTTTCCTATGTCAAGACTCTCACACAAACTGATACTAAATCTGGATTCAATATGCCTCGTCAATGCGCAGACTTGATCTTTCCAGAGTTGCAGAAGTCTCAAGAAGTCTCTGTCACTGACCTTCCAGGCAAAGTCTACACCTATACCTACTCAAAACAGAGTAGACTAACTAAAGGATGGTTTAATTTTATAAGTGAGAAGAAACTCGTCCCCGAAGATTCCGTTGTTTTCATCAAAAACTCTGCCGGGAAAATATATGTCGGAATTCGCAGGAACACTAACTTCCCTGCAGCTGAAAGAGGTGAGAAATTAACTGAGAGTGCGGTCTTGGGTGCAGTGGATTTGGCTAACAAGAACGTGGCGTTTGAAGTTGTGTTTTATCCCACAGATGGGAATTGGTGTAATTTTGTGGTGGATGCCAAGGTTGTAGATGATGCCATGAAGATTGGTTGGAAATGTGGAATGAGGGCAAAGCTTCGTTTGATGGAACCTGAATCTTCAAATTTAACTATAAGAGGTTTTCAGCGGGAGGGGACAATCTCTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTATGGTACTTATGCTTTCTTATTTTGTTCTAATGGTATTTCAATTGCATATTGTTATCAAGTTGAATCCATAATAAACAAGATTGATTTAGATATTAAAGCTATAATTAGATGATTCTTGATGAAATAGATATAAAACTTTGAGACTTAGTAGGTTGCTACTGTTTTGAATGTAGGCTTGCTTTGTGCCCTATAGAATAAGTGTCAAATGTAATTCTAAAATTAAGAAACACAAAATCATTACAGAATATCCATCTGCTACATTATTATTTCCATTGTTCTTGATATCTCATGACCGTTAATGTAAGTAGATTACGTCTTAGAATCAATTGTTTGTATCACACCTAAGTAGAATCTCTATTTTGGCTCATGTTATTAATTGTTATGAAACTTATTAATTGATGTCTAAATACATATATATACTAAGTTATCAAATATTTCAAATTATTGATATTGATTTCCATTTTTTATCTCTTTATCATAGGTTAAATGGGATGAACCTAATACCCCGCAGAAACCACCCCTGGTCAATCCCTGGGAGGTTGAAGTTTACCCGTCAACTTCCTCTTCCACTGTCAACATCTCCACAACAAAAAGTCCTAAACTTTCTCAGAGTTCTTCTCCCTGGCAAATTCAAAATTCTTCAATGCCAAGTACACTGACACCATTACCACCTCCAGTGCTTATTTTTCCATCTGGCATGCAAGGAGTCAGGCAAGAAGGTGCTGACTTGTTCGCAGGGTCGAGTGGTACTTTGCTTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGATGCTGAATTGTTCGCAGAGTTGAGTTGTACTTTGCCTGATACAAGGCTAAAGGTTGGCAGTTCTAAAGATGTTGACTTGTTTGCAGAGTCGAGTGGTACTTTGCCTGATACAAGACTAAAGCTTGGCAGTTCTAAAGGCGCTAACTTGGGCGCAGAGTCGACTGATACTTTGGCTGATACAGAGCTAAAGCTTGGCAGTTCTACAGGTGCGGACTTGGGCGCAGAGTCGAGTGATACTTTGGCTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGCGCAGAGTCGAGTGATACTTTGGCTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGCGCAGAGTCGAGTGATACTTTGGCTGATACAGTGCTAAAGCTTGGCAGTTCTAAAGGTGCTGAGTTGTTGCCTCATAGGTCCAATGAGCCAAGCACCAAGTGA
Protein sequence

>MS.gene053715.t1

MDSKLWQKCAGPDVKIPKIHSKVYYFPQGHLEHACSSPNAAPITILDGYRPSIPCIVSTVDLFVDPRTDEVFAKLILTPVTDQEPPPPVVHGQEDEDGNNLVSYVKTLTQTDTKSGFNMPRQCADLIFPELQKSQEVSVTDLPGKVYTYTYSKQSRLTKGWFNFISEKKLVPEDSVVFIKNSAGKIYVGIRRNTNFPAAERGEKLTESAVLGAVDLANKNVAFEVVFYPTDGNWCNFVVDAKVVDDAMKIGWKCGMRAKLRLMEPESSNLTIRGFQREGTISNDSSNVPNWRMLMVKWDEPNTPQKPPLVNPWEVEVYPSTSSSTVNISTTKSPKLSQSSSPWQIQNSSMPSTLTPLPPPVLIFPSGMQGVRQEGADLFAGSSGTLLDTGLKLGSSKDAELFAELSCTLPDTRLKVGSSKDVDLFAESSGTLPDTRLKLGSSKGANLGAESTDTLADTELKLGSSTGADLGAESSDTLADTGLKLGSSKGADLGAESSDTLADTGLKLGSSKGADLGAESSDTLADTVLKLGSSKGAELLPHRSNEPSTK