AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053717

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053717.t1 MTR_5g061890 91.821 379 23 2 1 376 1 374 0.0 707
MS.gene053717.t1 MTR_5g060780 90.501 379 28 2 1 376 1 374 0.0 694
MS.gene053717.t1 MTR_5g460920 90.501 379 28 2 1 376 1 374 0.0 693
MS.gene053717.t1 MTR_5g060630 55.156 417 134 8 1 367 1 414 3.83e-134 394
MS.gene053717.t1 MTR_5g460740 55.156 417 134 8 1 367 1 414 3.83e-134 394
MS.gene053717.t1 MTR_5g061220 54.430 395 139 9 1 364 8 392 7.70e-131 385
MS.gene053717.t1 MTR_1g058210 54.068 381 139 10 1 364 1 362 3.56e-121 358
MS.gene053717.t1 MTR_2g024430 52.661 357 135 16 1 341 9 347 2.10e-104 317
MS.gene053717.t1 MTR_5g040880 49.408 338 148 8 8 335 7 331 1.82e-97 300
MS.gene053717.t1 MTR_5g040740 51.807 332 125 11 1 315 1 314 1.29e-90 278
MS.gene053717.t1 MTR_2g006270 44.792 384 181 13 6 374 7 374 7.94e-89 275
MS.gene053717.t1 MTR_5g082140 45.954 346 158 10 6 336 6 337 7.03e-88 273
MS.gene053717.t1 MTR_5g060770 53.094 307 88 7 61 365 7 259 3.98e-85 263
MS.gene053717.t1 MTR_2g006380 42.782 381 186 15 6 374 14 374 1.39e-83 262
MS.gene053717.t1 MTR_5g460910 47.003 317 89 10 52 365 14 254 2.17e-65 211
MS.gene053717.t1 MTR_5g062970 58.673 196 65 7 12 195 38 229 2.77e-64 205
MS.gene053717.t1 MTR_3g073420 34.048 420 199 11 9 378 16 407 2.90e-63 213
MS.gene053717.t1 MTR_1g094960 35.049 408 193 15 9 361 16 406 4.56e-58 199
MS.gene053717.t1 MTR_1g064430 36.253 411 191 14 9 360 37 435 2.95e-57 198
MS.gene053717.t1 MTR_1g064430 36.253 411 191 14 9 360 24 422 2.99e-57 198
MS.gene053717.t1 MTR_2g094570 35.092 379 185 12 9 336 8 376 2.23e-56 196
MS.gene053717.t1 MTR_2g094570 35.092 379 185 12 9 336 8 376 2.45e-56 195
MS.gene053717.t1 MTR_2g094570 35.092 379 185 12 9 336 64 432 4.92e-56 196
MS.gene053717.t1 MTR_4g058930 35.385 390 184 13 9 340 9 388 2.24e-54 190
MS.gene053717.t1 MTR_4g058930 35.897 390 182 13 9 340 9 388 2.74e-54 190
MS.gene053717.t1 MTR_7g101280 30.617 405 190 13 9 341 28 413 6.48e-49 175
MS.gene053717.t1 MTR_0041s0110 30.617 405 190 13 9 341 28 413 6.48e-49 175
MS.gene053717.t1 MTR_2g014770 29.897 388 228 15 4 364 43 413 5.93e-41 152
MS.gene053717.t1 MTR_7g101275 32.492 317 172 11 22 314 2 300 1.05e-40 148
MS.gene053717.t1 MTR_2g014770 29.897 388 228 15 4 364 43 413 1.24e-40 152
MS.gene053717.t1 MTR_5g062960 63.393 112 40 1 227 338 1 111 1.30e-39 140
MS.gene053717.t1 MTR_4g021580 28.691 359 236 8 12 358 19 369 1.11e-37 143
MS.gene053717.t1 MTR_2g005240 28.497 386 231 10 12 371 23 389 1.49e-37 143
MS.gene053717.t1 MTR_4g021580 28.691 359 236 8 12 358 19 369 1.95e-37 143
MS.gene053717.t1 MTR_0041s0120 31.595 326 175 12 22 314 2 312 1.19e-36 137
MS.gene053717.t1 MTR_7g062540 28.611 360 238 8 12 359 14 366 1.52e-36 140
MS.gene053717.t1 MTR_8g100050 29.586 338 219 8 12 335 59 391 8.43e-36 139
MS.gene053717.t1 MTR_8g100050 29.586 338 219 8 12 335 59 391 1.85e-35 138
MS.gene053717.t1 MTR_2g018690 29.942 344 218 10 9 335 20 357 3.71e-35 137
MS.gene053717.t1 MTR_4g088210 29.793 386 224 15 12 369 38 404 1.13e-34 135
MS.gene053717.t1 MTR_4g088210 29.793 386 224 15 12 369 38 404 1.41e-34 135
MS.gene053717.t1 MTR_8g027440 29.333 375 243 11 12 373 29 394 2.35e-34 134
MS.gene053717.t1 MTR_4g124900 28.779 344 220 10 9 335 20 355 3.20e-32 129
MS.gene053717.t1 MTR_4g124900 28.818 347 216 10 9 335 20 355 3.71e-32 128
MS.gene053717.t1 MTR_5g076270 29.619 341 217 10 12 335 24 358 4.28e-32 128
MS.gene053717.t1 MTR_2g043250 29.360 344 218 11 9 335 20 355 4.73e-32 128
MS.gene053717.t1 MTR_1g024025 30.205 341 214 11 12 335 43 376 1.15e-31 127
MS.gene053717.t1 MTR_8g079492 28.531 354 209 12 9 335 20 356 1.55e-31 126
MS.gene053717.t1 MTR_8g079492 28.531 354 209 12 9 335 20 356 1.62e-31 126
MS.gene053717.t1 MTR_3g064050 29.326 341 218 10 12 335 26 360 5.09e-31 125
MS.gene053717.t1 MTR_2g093740 29.397 398 233 15 12 376 56 438 3.80e-30 122
MS.gene053717.t1 MTR_2g093740 29.397 398 233 15 12 376 56 438 4.90e-30 122
MS.gene053717.t1 MTR_2g093740 29.397 398 233 15 12 376 56 438 6.51e-30 122
MS.gene053717.t1 MTR_4g060460 28.571 399 234 15 12 376 54 435 4.77e-28 116
MS.gene053717.t1 MTR_8g101360 28.291 357 207 11 9 335 28 365 1.81e-27 114
MS.gene053717.t1 MTR_2g093740 28.467 411 233 16 12 376 56 451 4.30e-27 113
MS.gene053717.t1 MTR_2g093740 28.467 411 233 16 12 376 56 451 5.23e-27 113
MS.gene053717.t1 MTR_2g093740 28.467 411 233 16 12 376 56 451 6.07e-27 113
MS.gene053717.t1 MTR_4g060460 28.462 390 236 15 12 371 54 430 7.63e-27 112
MS.gene053717.t1 MTR_8g079492 27.178 287 175 9 70 335 1 274 8.63e-20 91.7
MS.gene053717.t1 MTR_8g446900 27.181 298 173 12 61 335 12 288 3.76e-19 88.6
MS.gene053717.t1 MTR_2g093740 28.435 313 185 13 88 376 11 308 1.53e-15 78.6
MS.gene053717.t1 MTR_2g093740 28.617 311 183 13 90 376 13 308 1.87e-15 78.6
MS.gene053717.t1 MTR_5g046120 35.897 117 69 3 50 160 12 128 4.85e-13 66.2
MS.gene053717.t1 MTR_2g093740 27.469 324 183 14 90 376 13 321 1.32e-12 69.7
MS.gene053717.t1 MTR_2g093740 27.469 324 183 14 90 376 13 321 1.55e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053717.t1 AT1G77850 34.759 374 189 12 8 336 15 378 4.64e-63 212
MS.gene053717.t1 AT1G77850 34.759 374 189 12 8 336 15 378 4.69e-63 212
MS.gene053717.t1 AT4G30080 35.806 391 190 14 9 347 16 397 6.09e-54 189
MS.gene053717.t1 AT2G28350 33.503 394 187 14 9 340 7 387 1.20e-51 182
MS.gene053717.t1 AT2G28350 33.503 394 187 14 9 340 7 387 1.49e-51 183
MS.gene053717.t1 AT2G46530 30.328 366 229 8 12 361 21 376 3.55e-43 159
MS.gene053717.t1 AT2G46530 30.328 366 229 8 12 361 42 397 3.82e-43 159
MS.gene053717.t1 AT3G61830 30.857 350 210 11 12 342 25 361 3.61e-42 155
MS.gene053717.t1 AT1G34390 30.563 373 220 12 12 364 27 380 1.68e-39 148
MS.gene053717.t1 AT1G59750 31.675 382 230 13 12 374 22 391 7.05e-39 147
MS.gene053717.t1 AT1G59750 31.967 366 218 13 12 358 22 375 1.02e-38 147
MS.gene053717.t1 AT1G59750 31.967 366 218 13 12 358 22 375 1.02e-38 147
MS.gene053717.t1 AT1G35520 31.332 383 206 15 12 364 27 382 1.15e-38 146
MS.gene053717.t1 AT1G59750 31.967 366 218 13 12 358 22 375 1.31e-38 146
MS.gene053717.t1 AT1G34410 30.446 381 212 15 12 364 27 382 2.80e-37 142
MS.gene053717.t1 AT1G34310 30.990 384 212 15 9 364 24 382 1.09e-36 140
MS.gene053717.t1 AT1G35540 31.347 386 202 19 12 364 27 382 3.15e-36 139
MS.gene053717.t1 AT5G62000 29.107 347 209 10 12 335 61 393 7.47e-36 139
MS.gene053717.t1 AT5G62000 29.107 347 209 10 12 335 61 393 7.88e-36 139
MS.gene053717.t1 AT5G62000 29.107 347 209 10 12 335 61 393 7.88e-36 139
MS.gene053717.t1 AT5G62000 29.107 347 209 10 12 335 61 393 7.88e-36 139
MS.gene053717.t1 AT5G62000 29.107 347 209 10 12 335 61 393 7.88e-36 139
MS.gene053717.t1 AT4G23980 28.818 347 222 9 12 340 12 351 2.30e-35 137
MS.gene053717.t1 AT4G23980 28.818 347 222 9 12 340 12 351 3.00e-35 137
MS.gene053717.t1 AT1G35240 30.854 363 195 15 12 343 27 364 6.32e-35 135
MS.gene053717.t1 AT5G37020 30.814 344 209 13 12 335 22 356 3.80e-34 134
MS.gene053717.t1 AT1G30330 29.706 340 218 9 12 335 23 357 4.92e-34 134
MS.gene053717.t1 AT1G30330 29.706 340 218 9 12 335 25 359 4.93e-34 134
MS.gene053717.t1 AT1G30330 29.706 340 218 9 12 335 25 359 4.93e-34 134
MS.gene053717.t1 AT5G37020 30.814 344 209 13 12 335 22 356 5.95e-34 134
MS.gene053717.t1 AT1G34170 30.471 361 208 14 12 349 25 365 1.92e-33 130
MS.gene053717.t1 AT2G33860 29.306 389 228 13 12 378 54 417 5.17e-33 130
MS.gene053717.t1 AT1G19850 30.347 346 218 10 7 335 38 377 1.23e-30 124
MS.gene053717.t1 AT1G19850 30.347 346 218 10 7 335 49 388 1.41e-30 124
MS.gene053717.t1 AT1G19220 28.448 348 216 10 9 335 20 355 1.45e-30 124
MS.gene053717.t1 AT5G20730 29.023 348 215 11 9 335 21 357 9.48e-30 121
MS.gene053717.t1 AT5G20730 29.023 348 215 11 9 335 21 357 9.61e-30 121
MS.gene053717.t1 AT3G61830 30.769 299 180 9 59 342 24 310 1.09e-29 120
MS.gene053717.t1 AT5G20730 28.530 347 217 10 9 335 21 356 2.35e-29 120
MS.gene053717.t1 AT5G60450 29.060 351 212 13 12 335 41 381 1.75e-28 117
MS.gene053717.t1 AT5G60450 29.060 351 212 13 12 335 65 405 1.87e-28 117
MS.gene053717.t1 AT1G43950 32.843 204 117 6 12 203 27 222 2.89e-27 108
MS.gene053717.t1 AT1G34170 28.492 358 193 13 12 349 25 339 4.37e-27 112
MS.gene053717.t1 AT1G34170 28.492 358 193 13 12 349 25 339 7.08e-27 112
MS.gene053717.t1 AT2G46530 29.496 278 174 7 94 361 24 289 3.69e-24 104
MS.gene053717.t1 AT2G33860 28.674 279 167 10 112 378 2 260 2.03e-19 89.7
MS.gene053717.t1 AT5G37020 29.545 264 165 10 85 335 6 261 2.35e-17 84.3

Find 101 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGCATATCTGCCATATTT+TGG 0.143600 5.1:-46558990 None:intergenic
ATGACAATTTCTCAGCTTAA+AGG 0.194158 5.1:+46559820 MS.gene053717:CDS
TGTCTCCATCATCGTCTTCT+TGG 0.236516 5.1:-46559258 None:intergenic
AACTGATGAGATTAAGGTTT+TGG 0.252605 5.1:+46559618 MS.gene053717:CDS
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.1:-46559031 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.1:+46559866 MS.gene053717:CDS
GAATTTAACTGATGAGATTA+AGG 0.298587 5.1:+46559612 MS.gene053717:CDS
CGACCCTGCCAAGGTTTGTT+GGG 0.312042 5.1:-46560488 None:intergenic
TGCCAAGGATTGACTAGGTT+TGG 0.313165 5.1:-46560370 None:intergenic
GATCTTTCCCAAGCTTGATT+TGG 0.314401 5.1:+46559360 MS.gene053717:CDS
ACCTCAAGCATGCGCCAATT+AGG 0.318991 5.1:-46559880 None:intergenic
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5.1:+46559748 MS.gene053717:CDS
CTACTTCCCACGAGGTCATC+TGG 0.355762 5.1:+46559054 MS.gene053717:CDS
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5.1:-46559879 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5.1:-46559016 None:intergenic
TCCATCATCGTCTTCTTGGC+CGG 0.379595 5.1:-46559254 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5.1:-46559746 None:intergenic
TCGACCCTGCCAAGGTTTGT+TGG 0.395481 5.1:-46560489 None:intergenic
CTCGTGTGGAACCCAAAATA+TGG 0.402726 5.1:+46558978 MS.gene053717:CDS
TGACAATTTCTCAGCTTAAA+GGG 0.407434 5.1:+46559821 MS.gene053717:CDS
GCCGGGAACTACAGGAGGAG+GGG 0.417463 5.1:-46559236 None:intergenic
CGCTTCATCTGTAGTGAACT+TGG 0.424216 5.1:-46559575 None:intergenic
AATTCCGACAGATATCTTTC+CGG 0.425831 5.1:-46559542 None:intergenic
CTTGGTCAGTTACGGGAGTC+AGG 0.426329 5.1:-46559210 None:intergenic
TGGCCGGGAACTACAGGAGG+AGG 0.428083 5.1:-46559238 None:intergenic
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5.1:-46559011 None:intergenic
CGACCTTAAAATCCGAGAAT+GGG 0.449393 5.1:+46559408 MS.gene053717:CDS
TCACCCCAACAAACCTTGGC+AGG 0.450090 5.1:+46560484 MS.gene053717:CDS
GACTCGTCCAAATCAAGCTT+GGG 0.456946 5.1:-46559367 None:intergenic
GTGTATTATCCGACAGCTAT+TGG 0.461034 5.1:+46559682 MS.gene053717:CDS
TCTTCTTGGCCGGGAACTAC+AGG 0.462528 5.1:-46559244 None:intergenic
CCATCATCGTCTTCTTGGCC+GGG 0.465753 5.1:-46559253 None:intergenic
TACGGGAGTCAGGAGCAGTT+TGG 0.469490 5.1:-46559200 None:intergenic
CCGGGAACTACAGGAGGAGG+GGG 0.475602 5.1:-46559235 None:intergenic
CCATGGTTTGGCTATGTGTC+AGG 0.476593 5.1:-46560523 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5.1:+46559736 MS.gene053717:CDS
GATGAAAACAACAGAATCGT+TGG 0.490573 5.1:-46559509 None:intergenic
AGACTCGTCCAAATCAAGCT+TGG 0.490804 5.1:-46559368 None:intergenic
GAAGATTCATATTTCTTCAA+AGG 0.492904 5.1:-46559787 None:intergenic
ATGGTTCTTGTGGCAGCAGT+AGG 0.493323 5.1:-46559094 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.493859 5.1:+46560328 MS.gene053717:intron
TGAGGCCGAGTTGACGGTGG+AGG 0.494772 5.1:-46560431 None:intergenic
GGCCGAGTTGACGGTGGAGG+AGG 0.496620 5.1:-46560428 None:intergenic
CCGACCTTAAAATCCGAGAA+TGG 0.506666 5.1:+46559407 MS.gene053717:CDS
ATCCAAACCTAGTCAATCCT+TGG 0.508187 5.1:+46560368 MS.gene053717:CDS
CAACCTGCCAAGGATTGACT+AGG 0.513736 5.1:-46560375 None:intergenic
GAGACGATGCATGGAATGGA+TGG 0.515792 5.1:-46559133 None:intergenic
TCATCCTCTGCCACCTCGTG+TGG 0.517268 5.1:+46558964 MS.gene053717:CDS
AATCTCAGTAGATTACATAC+TGG 0.519388 5.1:+46559451 MS.gene053717:CDS
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5.1:+46559879 MS.gene053717:CDS
GGTTCCACACGAGGTGGCAG+AGG 0.527487 5.1:-46558968 None:intergenic
GGAAGAGACGATGCATGGAA+TGG 0.530637 5.1:-46559137 None:intergenic
TGGTTCTTGTGGCAGCAGTA+GGG 0.533159 5.1:-46559093 None:intergenic
TTCATCACCCCAACAAACCT+TGG 0.536178 5.1:+46560480 MS.gene053717:CDS
TGACTAGGTTTGGATTCTGC+GGG 0.536979 5.1:-46560360 None:intergenic
GATGAACTCTCAGCAAGTCT+AGG 0.537540 5.1:-46560463 None:intergenic
GAGGAGGAAGTCGAAGGTGC+AGG 0.538875 5.1:-46560412 None:intergenic
AGATATCTGTCGGAATTCGC+AGG 0.543135 5.1:+46559548 MS.gene053717:CDS
ACTACAGATGAAGCGGATGA+AGG 0.543135 5.1:+46559583 MS.gene053717:CDS
TTTGGATGCAGTGGAATTGG+CGG 0.544683 5.1:+46559636 MS.gene053717:CDS
TTGTAAACTTCAACCTGCCA+AGG 0.544687 5.1:-46560385 None:intergenic
AAACCTAGTCAATCCTTGGC+AGG 0.546100 5.1:+46560372 MS.gene053717:CDS
ACGATGCCATGAAGATTGGT+TGG 0.558168 5.1:+46559740 MS.gene053717:CDS
TCAGTAGATTACATACTGGC+TGG 0.560711 5.1:+46559455 MS.gene053717:CDS
CCCGGCCAAGAAGACGATGA+TGG 0.562611 5.1:+46559253 MS.gene053717:CDS
GGTTCTTGTGGCAGCAGTAG+GGG 0.568653 5.1:-46559092 None:intergenic
GACCCTGCCAAGGTTTGTTG+GGG 0.572005 5.1:-46560487 None:intergenic
ATTATCCGACAGCTATTGGT+TGG 0.574808 5.1:+46559686 MS.gene053717:CDS
GGCCGGGAACTACAGGAGGA+GGG 0.579671 5.1:-46559237 None:intergenic
TTCCTCCTCCACCGTCAACT+CGG 0.580429 5.1:+46560426 MS.gene053717:CDS
TTGACTAGGTTTGGATTCTG+CGG 0.581555 5.1:-46560361 None:intergenic
TGGACGGTAACGATCAAGAA+TGG 0.583957 5.1:-46559113 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.584809 5.1:+46559576 MS.gene053717:CDS
AAAATATGGCAGATATGCGC+CGG 0.589425 5.1:+46558992 MS.gene053717:CDS
GACCTTAAAATCCGAGAATG+GGG 0.592494 5.1:+46559409 MS.gene053717:CDS
TAATCTACTGAGATTTGCAT+AGG 0.604127 5.1:-46559443 None:intergenic
TCAACGGAAGAGACGATGCA+TGG 0.604313 5.1:-46559142 None:intergenic
GCAAAGACCTCGTCGGTGTG+AGG 0.608714 5.1:-46559178 None:intergenic
CAAACCTTGGCAGGGTCGAG+TGG 0.615213 5.1:+46560493 MS.gene053717:CDS
CGATGCATGGAATGGATGGA+CGG 0.620709 5.1:-46559129 None:intergenic
ACAGATGAAGCGGATGAAGG+AGG 0.630115 5.1:+46559586 MS.gene053717:CDS
AATCTTTGGAATCTTGACGT+CGG 0.630358 5.1:-46559017 None:intergenic
ACGATCAAGAATGGTTCTTG+TGG 0.631537 5.1:-46559104 None:intergenic
TCTTGAGGCCGAGTTGACGG+TGG 0.632846 5.1:-46560434 None:intergenic
TCACGCCAACCAATAGCTGT+CGG 0.635734 5.1:-46559691 None:intergenic
TTGTGTGAGAGTCTTGACAT+AGG 0.637006 5.1:-46559290 None:intergenic
AGTACCACTCGACCCTGCCA+AGG 0.642496 5.1:-46560497 None:intergenic
TCTGCCGGAAAGATATCTGT+CGG 0.642648 5.1:+46559538 MS.gene053717:CDS
CACCCCAACAAACCTTGGCA+GGG 0.644050 5.1:+46560485 MS.gene053717:CDS
AGGATCAACAAAGAGATCAA+CGG 0.651533 5.1:-46559158 None:intergenic
TGTTGATCCTCACACCGACG+AGG 0.655379 5.1:+46559171 MS.gene053717:CDS
TCTTGGCCGGGAACTACAGG+AGG 0.657068 5.1:-46559241 None:intergenic
CAGTTTGGCAAAGACCTCGT+CGG 0.663811 5.1:-46559185 None:intergenic
AGGTGGCAGAGGATGAGACA+TGG 0.663852 5.1:-46558957 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5.1:-46559015 None:intergenic
ATCAAGTTAGAGCATTCGAT+AGG 0.670484 5.1:-46559340 None:intergenic
ACCTTAAAATCCGAGAATGG+GGG 0.676081 5.1:+46559410 MS.gene053717:CDS
CATGTTCCAGATGACCTCGT+GGG 0.687319 5.1:-46559060 None:intergenic
GCATGTTCCAGATGACCTCG+TGG 0.693178 5.1:-46559061 None:intergenic
ACGGTGGAGGAGGAAGTCGA+AGG 0.704506 5.1:-46560418 None:intergenic
AAAGTTTACTACTTCCCACG+AGG 0.761653 5.1:+46559046 MS.gene053717:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTATCTTTTTATCAT+AGG + chr5.1:46560315-46560334 MS.gene053717:intron 15.0%
!! GAAATATTATTAATTCCCAT+TGG + chr5.1:46560059-46560078 MS.gene053717:intron 20.0%
!! TCAATCAAATAATCTTATCA+TGG - chr5.1:46559948-46559967 None:intergenic 20.0%
! AAAAGTTTCTACTTAGCATT+AGG - chr5.1:46560226-46560245 None:intergenic 25.0%
! GAAGATTCATATTTCTTCAA+AGG - chr5.1:46559790-46559809 None:intergenic 25.0%
! GAATTTAACTGATGAGATTA+AGG + chr5.1:46559612-46559631 MS.gene053717:CDS 25.0%
! GTACATATGCTTTCTTAATT+TGG + chr5.1:46559901-46559920 MS.gene053717:intron 25.0%
!! CTTTTTATCATAGGTTAACT+GGG + chr5.1:46560324-46560343 MS.gene053717:intron 25.0%
!! TCTTTTTATCATAGGTTAAC+TGG + chr5.1:46560323-46560342 MS.gene053717:intron 25.0%
!!! CTATTTTTAGTGAATATCAC+TGG - chr5.1:46560158-46560177 None:intergenic 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG - chr5.1:46559034-46559053 None:intergenic 25.0%
AATCTCAGTAGATTACATAC+TGG + chr5.1:46559451-46559470 MS.gene053717:CDS 30.0%
ATGACAATTTCTCAGCTTAA+AGG + chr5.1:46559820-46559839 MS.gene053717:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG + chr5.1:46559866-46559885 MS.gene053717:CDS 30.0%
TAATCTACTGAGATTTGCAT+AGG - chr5.1:46559446-46559465 None:intergenic 30.0%
TGACAATTTCTCAGCTTAAA+GGG + chr5.1:46559821-46559840 MS.gene053717:CDS 30.0%
! AACTGATGAGATTAAGGTTT+TGG + chr5.1:46559618-46559637 MS.gene053717:CDS 30.0%
!! AGGAGTATTTACAATGAGAA+AGG - chr5.1:46560206-46560225 None:intergenic 30.0%
AATTCCGACAGATATCTTTC+CGG - chr5.1:46559545-46559564 None:intergenic 35.0%
AGATTTCCAACCAATCTTCA+TGG - chr5.1:46559749-46559768 None:intergenic 35.0%
AGGATCAACAAAGAGATCAA+CGG - chr5.1:46559161-46559180 None:intergenic 35.0%
GATGAAAACAACAGAATCGT+TGG - chr5.1:46559512-46559531 None:intergenic 35.0%
TACAATGAGAAAGGCTTAGA+AGG - chr5.1:46560197-46560216 None:intergenic 35.0%
TTATCATAGGTTAACTGGGA+TGG + chr5.1:46560328-46560347 MS.gene053717:intron 35.0%
! AAGTCTAGGAGTTTTTCTTG+AGG - chr5.1:46560452-46560471 None:intergenic 35.0%
! ATCAAGTTAGAGCATTCGAT+AGG - chr5.1:46559343-46559362 None:intergenic 35.0%
! ATGAAGATTGGTTGGAAATC+TGG + chr5.1:46559748-46559767 MS.gene053717:CDS 35.0%
! GTTTTCATCAAAAACTCTGC+CGG + chr5.1:46559523-46559542 MS.gene053717:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG - chr5.1:46559020-46559039 None:intergenic 35.0%
AAAATATGGCAGATATGCGC+CGG + chr5.1:46558992-46559011 MS.gene053717:CDS 40.0%
AAAGTTTACTACTTCCCACG+AGG + chr5.1:46559046-46559065 MS.gene053717:CDS 40.0%
ACCTTAAAATCCGAGAATGG+GGG + chr5.1:46559410-46559429 MS.gene053717:CDS 40.0%
ACGATCAAGAATGGTTCTTG+TGG - chr5.1:46559107-46559126 None:intergenic 40.0%
ATCCAAACCTAGTCAATCCT+TGG + chr5.1:46560368-46560387 MS.gene053717:CDS 40.0%
ATTATCCGACAGCTATTGGT+TGG + chr5.1:46559686-46559705 MS.gene053717:CDS 40.0%
CAAGTTCACTACAGATGAAG+CGG + chr5.1:46559576-46559595 MS.gene053717:CDS 40.0%
CGACCTTAAAATCCGAGAAT+GGG + chr5.1:46559408-46559427 MS.gene053717:CDS 40.0%
CTATCAAGTGCAAGACCAAT+GGG - chr5.1:46560077-46560096 None:intergenic 40.0%
GACCTTAAAATCCGAGAATG+GGG + chr5.1:46559409-46559428 MS.gene053717:CDS 40.0%
GATCTTTCCCAAGCTTGATT+TGG + chr5.1:46559360-46559379 MS.gene053717:CDS 40.0%
GTGTATTATCCGACAGCTAT+TGG + chr5.1:46559682-46559701 MS.gene053717:CDS 40.0%
TATATGTGTACCCCCATTCT+CGG - chr5.1:46559423-46559442 None:intergenic 40.0%
TCAGTAGATTACATACTGGC+TGG + chr5.1:46559455-46559474 MS.gene053717:CDS 40.0%
TCTATCAAGTGCAAGACCAA+TGG - chr5.1:46560078-46560097 None:intergenic 40.0%
TTGACTAGGTTTGGATTCTG+CGG - chr5.1:46560364-46560383 None:intergenic 40.0%
TTGTAAACTTCAACCTGCCA+AGG - chr5.1:46560388-46560407 None:intergenic 40.0%
TTGTGTGAGAGTCTTGACAT+AGG - chr5.1:46559293-46559312 None:intergenic 40.0%
! GCGCATATCTGCCATATTTT+GGG - chr5.1:46558992-46559011 None:intergenic 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG - chr5.1:46559019-46559038 None:intergenic 40.0%
!! TATTGGTTGGCGTGATTTTG+TGG + chr5.1:46559699-46559718 MS.gene053717:CDS 40.0%
!!! CCATTCTCGGATTTTAAGGT+CGG - chr5.1:46559410-46559429 None:intergenic 40.0%
!!! GATTAAGGTTTTGGATGCAG+TGG + chr5.1:46559627-46559646 MS.gene053717:CDS 40.0%
AAACCTAGTCAATCCTTGGC+AGG + chr5.1:46560372-46560391 MS.gene053717:CDS 45.0%
ACGATGCCATGAAGATTGGT+TGG + chr5.1:46559740-46559759 MS.gene053717:CDS 45.0%
ACTACAGATGAAGCGGATGA+AGG + chr5.1:46559583-46559602 MS.gene053717:CDS 45.0%
AGACTCGTCCAAATCAAGCT+TGG - chr5.1:46559371-46559390 None:intergenic 45.0%
AGATATCTGTCGGAATTCGC+AGG + chr5.1:46559548-46559567 MS.gene053717:CDS 45.0%
CCGACCTTAAAATCCGAGAA+TGG + chr5.1:46559407-46559426 MS.gene053717:CDS 45.0%
CGCTTCATCTGTAGTGAACT+TGG - chr5.1:46559578-46559597 None:intergenic 45.0%
CTCGTGTGGAACCCAAAATA+TGG + chr5.1:46558978-46558997 MS.gene053717:CDS 45.0%
GACTCGTCCAAATCAAGCTT+GGG - chr5.1:46559370-46559389 None:intergenic 45.0%
GATGAACTCTCAGCAAGTCT+AGG - chr5.1:46560466-46560485 None:intergenic 45.0%
GTTGACGATGCCATGAAGAT+TGG + chr5.1:46559736-46559755 MS.gene053717:CDS 45.0%
TCTGCCGGAAAGATATCTGT+CGG + chr5.1:46559538-46559557 MS.gene053717:CDS 45.0%
TGACTAGGTTTGGATTCTGC+GGG - chr5.1:46560363-46560382 None:intergenic 45.0%
TGCCAAGGATTGACTAGGTT+TGG - chr5.1:46560373-46560392 None:intergenic 45.0%
TGGACGGTAACGATCAAGAA+TGG - chr5.1:46559116-46559135 None:intergenic 45.0%
TGTCTCCATCATCGTCTTCT+TGG - chr5.1:46559261-46559280 None:intergenic 45.0%
TTCATCACCCCAACAAACCT+TGG + chr5.1:46560480-46560499 MS.gene053717:CDS 45.0%
TTTGGATGCAGTGGAATTGG+CGG + chr5.1:46559636-46559655 MS.gene053717:CDS 45.0%
! ACCCCCATTCTCGGATTTTA+AGG - chr5.1:46559414-46559433 None:intergenic 45.0%
! GGCGCATATCTGCCATATTT+TGG - chr5.1:46558993-46559012 None:intergenic 45.0%
!! TCTTTGGAATCTTGACGTCG+GGG - chr5.1:46559018-46559037 None:intergenic 45.0%
!! TTTTCTTGAGGCCGAGTTGA+CGG - chr5.1:46560440-46560459 None:intergenic 45.0%
!!! CATATTTTGGGTTCCACACG+AGG - chr5.1:46558980-46558999 None:intergenic 45.0%
!!! GGTTTTGGATGCAGTGGAAT+TGG + chr5.1:46559633-46559652 MS.gene053717:CDS 45.0%
ACAGATGAAGCGGATGAAGG+AGG + chr5.1:46559586-46559605 MS.gene053717:CDS 50.0%
ACCTCAAGCATGCGCCAATT+AGG - chr5.1:46559883-46559902 None:intergenic 50.0%
AGGGGGTTCTTGGTCAGTTA+CGG - chr5.1:46559221-46559240 None:intergenic 50.0%
ATGGTTCTTGTGGCAGCAGT+AGG - chr5.1:46559097-46559116 None:intergenic 50.0%
CAACCTGCCAAGGATTGACT+AGG - chr5.1:46560378-46560397 None:intergenic 50.0%
CAGTTTGGCAAAGACCTCGT+CGG - chr5.1:46559188-46559207 None:intergenic 50.0%
CATGTTCCAGATGACCTCGT+GGG - chr5.1:46559063-46559082 None:intergenic 50.0%
CCTCAAGCATGCGCCAATTA+GGG - chr5.1:46559882-46559901 None:intergenic 50.0%
CGATGCATGGAATGGATGGA+CGG - chr5.1:46559132-46559151 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG - chr5.1:46559136-46559155 None:intergenic 50.0%
GGAAGAGACGATGCATGGAA+TGG - chr5.1:46559140-46559159 None:intergenic 50.0%
TCAACGGAAGAGACGATGCA+TGG - chr5.1:46559145-46559164 None:intergenic 50.0%
TCACGCCAACCAATAGCTGT+CGG - chr5.1:46559694-46559713 None:intergenic 50.0%
TCCATCATCGTCTTCTTGGC+CGG - chr5.1:46559257-46559276 None:intergenic 50.0%
TGGTTCTTGTGGCAGCAGTA+GGG - chr5.1:46559096-46559115 None:intergenic 50.0%
! TGGTTGGCGTGATTTTGTGG+TGG + chr5.1:46559702-46559721 MS.gene053717:CDS 50.0%
!!! ATTTTGGGTTCCACACGAGG+TGG - chr5.1:46558977-46558996 None:intergenic 50.0%
CACCCCAACAAACCTTGGCA+GGG + chr5.1:46560485-46560504 MS.gene053717:CDS 55.0%
CCATCATCGTCTTCTTGGCC+GGG - chr5.1:46559256-46559275 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTG+AGG + chr5.1:46559879-46559898 MS.gene053717:CDS 55.0%
CTACTTCCCACGAGGTCATC+TGG + chr5.1:46559054-46559073 MS.gene053717:CDS 55.0%
CTTGGTCAGTTACGGGAGTC+AGG - chr5.1:46559213-46559232 None:intergenic 55.0%
GCATGTTCCAGATGACCTCG+TGG - chr5.1:46559064-46559083 None:intergenic 55.0%
GGGGGTTCTTGGTCAGTTAC+GGG - chr5.1:46559220-46559239 None:intergenic 55.0%
GGTTCTTGTGGCAGCAGTAG+GGG - chr5.1:46559095-46559114 None:intergenic 55.0%
TACGGGAGTCAGGAGCAGTT+TGG - chr5.1:46559203-46559222 None:intergenic 55.0%
TCACCCCAACAAACCTTGGC+AGG + chr5.1:46560484-46560503 MS.gene053717:CDS 55.0%
TCGACCCTGCCAAGGTTTGT+TGG - chr5.1:46560492-46560511 None:intergenic 55.0%
TCTTCTTGGCCGGGAACTAC+AGG - chr5.1:46559247-46559266 None:intergenic 55.0%
TGTTGATCCTCACACCGACG+AGG + chr5.1:46559171-46559190 MS.gene053717:CDS 55.0%
TTCCTCCTCCACCGTCAACT+CGG + chr5.1:46560426-46560445 MS.gene053717:CDS 55.0%
! CGACCCTGCCAAGGTTTGTT+GGG - chr5.1:46560491-46560510 None:intergenic 55.0%
!! GACCCTGCCAAGGTTTGTTG+GGG - chr5.1:46560490-46560509 None:intergenic 55.0%
ACGGTGGAGGAGGAAGTCGA+AGG - chr5.1:46560421-46560440 None:intergenic 60.0%
AGTACCACTCGACCCTGCCA+AGG - chr5.1:46560500-46560519 None:intergenic 60.0%
CAAACCTTGGCAGGGTCGAG+TGG + chr5.1:46560493-46560512 MS.gene053717:CDS 60.0%
CCCGGCCAAGAAGACGATGA+TGG + chr5.1:46559253-46559272 MS.gene053717:CDS 60.0%
CTACAGGAGGAGGGGGTTCT+TGG - chr5.1:46559231-46559250 None:intergenic 60.0%
GCAAAGACCTCGTCGGTGTG+AGG - chr5.1:46559181-46559200 None:intergenic 60.0%
TCATCCTCTGCCACCTCGTG+TGG + chr5.1:46558964-46558983 MS.gene053717:CDS 60.0%
TCTTGGCCGGGAACTACAGG+AGG - chr5.1:46559244-46559263 None:intergenic 60.0%
TGGAATCTTGACGTCGGGGC+CGG - chr5.1:46559014-46559033 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+AGG - chr5.1:46560415-46560434 None:intergenic 60.0%
! TCTTGAGGCCGAGTTGACGG+TGG - chr5.1:46560437-46560456 None:intergenic 60.0%
CCCCCTCCTCCTGTAGTTCC+CGG + chr5.1:46559235-46559254 MS.gene053717:CDS 65.0%
CCGGGAACTACAGGAGGAGG+GGG - chr5.1:46559238-46559257 None:intergenic 65.0%
GCCGGGAACTACAGGAGGAG+GGG - chr5.1:46559239-46559258 None:intergenic 65.0%
GGCCGGGAACTACAGGAGGA+GGG - chr5.1:46559240-46559259 None:intergenic 65.0%
GGTTCCACACGAGGTGGCAG+AGG - chr5.1:46558971-46558990 None:intergenic 65.0%
TGGCCGGGAACTACAGGAGG+AGG - chr5.1:46559241-46559260 None:intergenic 65.0%
! TGAGGCCGAGTTGACGGTGG+AGG - chr5.1:46560434-46560453 None:intergenic 65.0%
! GGCCGAGTTGACGGTGGAGG+AGG - chr5.1:46560431-46560450 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 46558959 46560534 46558959 ID=MS.gene053717
chr5.1 mRNA 46558959 46560534 46558959 ID=MS.gene053717.t1;Parent=MS.gene053717
chr5.1 exon 46558959 46559900 46558959 ID=MS.gene053717.t1.exon1;Parent=MS.gene053717.t1
chr5.1 CDS 46558959 46559900 46558959 ID=cds.MS.gene053717.t1;Parent=MS.gene053717.t1
chr5.1 exon 46560337 46560534 46560337 ID=MS.gene053717.t1.exon2;Parent=MS.gene053717.t1
chr5.1 CDS 46560337 46560534 46560337 ID=cds.MS.gene053717.t1;Parent=MS.gene053717.t1
Gene Sequence

>MS.gene053717

ATGTCTCATCCTCTGCCACCTCGTGTGGAACCCAAAATATGGCAGATATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACGAGGTCATCTGGAACATGCGTGTTCTTCCCCTACTGCTGCCACAAGAACCATTCTTGATCGTTACCGTCCATCCATTCCATGCATCGTCTCTTCCGTTGATCTCTTTGTTGATCCTCACACCGACGAGGTCTTTGCCAAACTGCTCCTGACTCCCGTAACTGACCAAGAACCCCCTCCTCCTGTAGTTCCCGGCCAAGAAGACGATGATGGAGACAATCTTGTTTCCTATGTCAAGACTCTCACACAATCTGATTGTGCAAGAGTATTATGCGTACCTATCGAATGCTCTAACTTGATCTTTCCCAAGCTTGATTTGGACGAGTCTCAGAGCATCACTGTCACCGACCTTAAAATCCGAGAATGGGGGTACACATATACCTATGCAAATCTCAGTAGATTACATACTGGCTGGTTAAATTTTGTACGAGAGAAGAAACTCGTCGCCAACGATTCTGTTGTTTTCATCAAAAACTCTGCCGGAAAGATATCTGTCGGAATTCGCAGGAACACCAAGTTCACTACAGATGAAGCGGATGAAGGAGGCGAGAATTTAACTGATGAGATTAAGGTTTTGGATGCAGTGGAATTGGCGGAGAAGAACACAGCATTTGATGTTGTGTATTATCCGACAGCTATTGGTTGGCGTGATTTTGTGGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAACTTCCTTTGAAGAAATATGAATCTTCAAGTTCAAAGATGACAATTTCTCAGCTTAAAGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTACATATGCTTTCTTAATTTGGTCTGATTATATTTAGTTGAATCCATGATAAGATTATTTGATTGATATATATGAGAGTTGTTGAAGTTAATTCATGAAACTATAATTAGATGATACTATATGATACAGAAAAATGTAACAGAATCAGATTACACTTGAAATATTATTAATTCCCATTGGTCTTGCACTTGATAGATATCTCATGACACTTAATGTTTACTTTATGTAATCAGATTGTTTTTATACGAAACTGCCAGTGATATTCACTAAAAATAGTTATGAAGTTTAAGAACCTTCTAAGCCTTTCTCATTGTAAATACTCCTAATGCTAAGTAGAAACTTTTGATGTCTAAATACATATATACTATATATATTTTATCAAATATTTCAAATTATTGATATTGATTCATACTGTTTTTTATCTTTTTATCATAGGTTAACTGGGATGGACTTGATTTCCCGCAGAATCCAAACCTAGTCAATCCTTGGCAGGTTGAAGTTTACAACATACCTGCACCTTCGACTTCCTCCTCCACCGTCAACTCGGCCTCAAGAAAAACTCCTAGACTTGCTGAGAGTTCATCACCCCAACAAACCTTGGCAGGGTCGAGTGGTACTTTGCCTGACACATAG
Protein sequence

>MS.gene053717.t1

MSHPLPPRVEPKIWQICAGPDVKIPKIHSKVYYFPRGHLEHACSSPTAATRTILDRYRPSIPCIVSSVDLFVDPHTDEVFAKLLLTPVTDQEPPPPVVPGQEDDDGDNLVSYVKTLTQSDCARVLCVPIECSNLIFPKLDLDESQSITVTDLKIREWGYTYTYANLSRLHTGWLNFVREKKLVANDSVVFIKNSAGKISVGIRRNTKFTTDEADEGGENLTDEIKVLDAVELAEKNTAFDVVYYPTAIGWRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKYESSSSKMTISQLKGTISFVFNDSSNVPNWRMLEVNWDGLDFPQNPNLVNPWQVEVYNIPAPSTSSSTVNSASRKTPRLAESSSPQQTLAGSSGTLPDT