AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053874

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053874.t1 MTR_5g055260 76.950 282 35 5 1 280 1 254 7.32e-145 408
MS.gene053874.t1 MTR_4g021790 74.144 263 67 1 1 263 1 262 3.91e-139 394
MS.gene053874.t1 MTR_4g021855 63.576 302 100 2 1 293 1 301 1.25e-131 376
MS.gene053874.t1 MTR_4g021760 62.583 302 110 3 1 300 1 301 3.56e-128 368
MS.gene053874.t1 MTR_4g021845 71.560 218 61 1 1 218 1 217 7.04e-110 318
MS.gene053874.t1 MTR_1g032570 45.968 248 102 4 10 226 11 257 1.68e-66 219
MS.gene053874.t1 MTR_4g023980 65.942 138 46 1 55 192 35 171 2.94e-62 196
MS.gene053874.t1 MTR_2g034960 43.983 241 110 3 10 226 29 268 1.56e-58 198
MS.gene053874.t1 MTR_4g121020 43.852 244 108 4 10 226 31 272 1.03e-56 194
MS.gene053874.t1 MTR_4g121020 43.852 244 108 4 10 226 31 272 1.14e-56 194
MS.gene053874.t1 MTR_3g086100 40.408 245 117 5 10 226 13 256 2.76e-53 183
MS.gene053874.t1 MTR_8g105600 40.964 249 127 2 1 230 1 248 5.88e-52 180
MS.gene053874.t1 MTR_6g045327 38.558 319 168 8 1 309 1 301 1.00e-51 175
MS.gene053874.t1 MTR_8g079940 39.754 244 120 6 9 226 24 266 3.31e-50 174
MS.gene053874.t1 MTR_3g106220 38.956 249 119 4 10 226 8 255 9.54e-49 172
MS.gene053874.t1 MTR_4g131600 38.559 236 121 4 9 221 19 253 5.87e-48 169
MS.gene053874.t1 MTR_1g013170 38.956 249 121 5 7 226 2 248 1.49e-47 166
MS.gene053874.t1 MTR_2g450070 35.019 257 125 5 10 227 18 271 1.30e-44 161
MS.gene053874.t1 MTR_2g450070 35.019 257 125 5 10 227 18 271 1.50e-44 161
MS.gene053874.t1 MTR_7g117705 35.887 248 128 5 7 225 2 247 6.49e-44 156
MS.gene053874.t1 MTR_8g093040 43.049 223 107 6 10 230 14 218 1.69e-43 150
MS.gene053874.t1 MTR_1g013180 38.039 255 127 6 1 226 147 399 1.77e-43 158
MS.gene053874.t1 MTR_4g131580 36.290 248 132 4 10 232 17 263 1.86e-43 157
MS.gene053874.t1 MTR_4g131570 36.735 245 126 5 10 226 17 260 7.86e-43 155
MS.gene053874.t1 MTR_3g102590 35.909 220 113 4 10 226 8 202 5.10e-41 143
MS.gene053874.t1 MTR_6g016850 44.444 162 88 2 1 161 1 161 9.33e-41 140
MS.gene053874.t1 MTR_1g032570 41.799 189 79 3 68 226 1 188 1.08e-40 149
MS.gene053874.t1 MTR_3g102590 35.238 210 110 3 10 218 8 192 3.26e-38 134
MS.gene053874.t1 MTR_3g102600 35.321 218 104 3 10 226 8 189 4.85e-38 135
MS.gene053874.t1 MTR_3g102600 33.491 212 104 3 10 220 8 183 3.70e-34 124
MS.gene053874.t1 MTR_3g086100 37.255 204 98 5 68 242 1 203 6.95e-33 127
MS.gene053874.t1 MTR_3g102600 34.286 175 79 2 52 226 2 140 2.74e-29 110
MS.gene053874.t1 MTR_7g026400 35.294 204 111 6 45 231 19 218 1.14e-27 113
MS.gene053874.t1 MTR_4g121020 41.818 165 68 3 88 226 6 168 1.78e-27 112
MS.gene053874.t1 MTR_0450s0040 31.897 232 135 6 14 231 8 230 3.69e-26 106
MS.gene053874.t1 MTR_3g102600 31.953 169 79 2 52 220 2 134 3.72e-25 98.6
MS.gene053874.t1 MTR_2g016220 37.209 129 74 3 154 276 69 196 2.20e-16 79.3
MS.gene053874.t1 MTR_2g016220 37.209 129 74 3 154 276 69 196 2.87e-16 79.0
MS.gene053874.t1 MTR_5g041350 53.425 73 31 2 169 240 15 85 7.39e-16 77.4
MS.gene053874.t1 MTR_4g064730 50.685 73 35 1 155 226 116 188 9.80e-16 76.6
MS.gene053874.t1 MTR_7g098250 51.429 70 33 1 169 237 262 331 1.16e-15 77.8
MS.gene053874.t1 MTR_2g016220 36.842 133 73 5 154 276 69 200 2.06e-15 76.3
MS.gene053874.t1 MTR_1g013160 30.000 150 75 4 71 192 1 148 4.02e-15 72.0
MS.gene053874.t1 MTR_1g080330 47.126 87 43 2 165 250 171 255 5.32e-15 74.3
MS.gene053874.t1 MTR_6g032990 56.452 62 24 2 169 229 42 101 5.88e-15 75.1
MS.gene053874.t1 MTR_7g089010 57.627 59 25 0 168 226 90 148 5.98e-15 74.3
MS.gene053874.t1 MTR_7g115530 50.000 70 34 1 169 237 218 287 8.70e-15 73.9
MS.gene053874.t1 MTR_1g080330 47.126 87 43 2 165 250 171 255 1.13e-14 74.3
MS.gene053874.t1 MTR_7g115530 50.000 70 34 1 169 237 218 287 1.55e-14 73.6
MS.gene053874.t1 MTR_7g115530 50.000 70 34 1 169 237 218 287 2.06e-14 73.9
MS.gene053874.t1 MTR_0223s0040 53.968 63 28 1 161 222 10 72 2.36e-14 72.4
MS.gene053874.t1 MTR_2g086450 53.846 65 28 2 158 221 35 98 2.76e-14 73.2
MS.gene053874.t1 MTR_7g115530 61.111 54 20 1 169 221 218 271 5.82e-14 72.0
MS.gene053874.t1 MTR_8g086410 48.529 68 34 1 155 221 97 164 6.38e-14 72.4
MS.gene053874.t1 MTR_8g086410 48.529 68 34 1 155 221 95 162 6.83e-14 72.0
MS.gene053874.t1 MTR_3g450310 49.275 69 34 1 161 228 11 79 9.52e-14 69.3
MS.gene053874.t1 MTR_7g069660 55.357 56 24 1 167 221 69 124 9.74e-14 71.2
MS.gene053874.t1 MTR_1g112370 50.000 70 34 1 154 222 7 76 1.48e-13 70.5
MS.gene053874.t1 MTR_8g086410 43.210 81 41 2 155 230 97 177 1.81e-13 70.9
MS.gene053874.t1 MTR_7g088070 50.667 75 34 2 160 231 41 115 3.45e-13 68.9
MS.gene053874.t1 MTR_4g081710 59.259 54 21 1 169 221 17 70 4.41e-13 69.3
MS.gene053874.t1 MTR_7g088070 45.977 87 44 2 160 243 41 127 5.27e-13 68.6
MS.gene053874.t1 MTR_7g115530 59.615 52 20 1 169 219 218 269 1.62e-12 67.0
MS.gene053874.t1 MTR_2g027800 34.000 150 87 6 143 282 14 161 1.89e-12 67.0
MS.gene053874.t1 MTR_1g090670 55.172 58 25 1 165 221 40 97 1.90e-12 67.0
MS.gene053874.t1 MTR_2g090675 40.000 80 48 0 146 225 138 217 2.00e-12 67.8
MS.gene053874.t1 MTR_2g023580 50.877 57 27 1 167 222 53 109 2.54e-12 65.9
MS.gene053874.t1 MTR_2g027800 45.000 80 42 2 143 221 14 92 2.63e-12 66.6
MS.gene053874.t1 MTR_2g084230 47.619 63 32 1 161 222 89 151 3.61e-12 66.2
MS.gene053874.t1 MTR_2g084230 47.619 63 32 1 161 222 89 151 4.50e-12 66.6
MS.gene053874.t1 MTR_6g444980 55.172 58 25 1 165 221 31 88 4.90e-12 66.2
MS.gene053874.t1 MTR_6g444980 55.172 58 25 1 165 221 31 88 5.28e-12 66.2
MS.gene053874.t1 MTR_7g093030 35.433 127 67 5 167 289 210 325 5.97e-12 66.2
MS.gene053874.t1 MTR_4g098870 47.945 73 37 1 164 236 325 396 6.90e-12 66.2
MS.gene053874.t1 MTR_4g098870 47.945 73 37 1 164 236 305 376 7.15e-12 66.2
MS.gene053874.t1 MTR_7g093030 51.786 56 26 1 167 221 210 265 7.56e-12 65.1
MS.gene053874.t1 MTR_7g115530 58.824 51 20 1 169 218 218 268 9.47e-12 65.1
MS.gene053874.t1 MTR_4g078810 50.000 58 29 0 168 225 157 214 9.52e-12 65.9
MS.gene053874.t1 MTR_4g098870 47.945 73 37 1 164 236 325 396 9.71e-12 65.9
MS.gene053874.t1 MTR_4g098870 47.945 73 37 1 164 236 344 415 9.78e-12 65.9
MS.gene053874.t1 MTR_4g098870 47.945 73 37 1 164 236 305 376 1.02e-11 65.9
MS.gene053874.t1 MTR_1g053800 53.968 63 28 1 163 224 171 233 1.29e-11 64.7
MS.gene053874.t1 MTR_2g027860 53.425 73 30 3 169 237 49 121 1.48e-11 64.7
MS.gene053874.t1 MTR_5g014040 45.070 71 39 0 158 228 300 370 1.88e-11 65.1
MS.gene053874.t1 MTR_5g014040 29.921 127 83 4 10 134 14 136 5.65e-11 63.5
MS.gene053874.t1 MTR_1g053835 41.892 74 40 2 167 239 256 327 3.09e-11 64.3
MS.gene053874.t1 MTR_5g027440 40.964 83 42 2 140 221 2 78 3.29e-11 63.2
MS.gene053874.t1 MTR_5g017980 50.820 61 30 0 166 226 190 250 4.61e-11 63.2
MS.gene053874.t1 MTR_5g017980 50.820 61 30 0 166 226 189 249 4.71e-11 63.2
MS.gene053874.t1 MTR_5g014040 29.921 127 83 4 10 134 14 136 5.14e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053874.t1 AT3G16857 40.304 263 110 3 10 226 33 294 2.97e-55 190
MS.gene053874.t1 AT3G16857 40.304 263 110 3 10 226 33 294 3.50e-55 190
MS.gene053874.t1 AT2G01760 41.961 255 112 6 7 226 4 257 1.20e-54 182
MS.gene053874.t1 AT1G67710 40.408 245 116 5 11 226 8 251 2.05e-49 172
MS.gene053874.t1 AT2G01760 37.809 283 112 7 7 226 4 285 1.08e-48 167
MS.gene053874.t1 AT2G25180 39.004 241 122 5 10 226 13 252 1.92e-45 162
MS.gene053874.t1 AT4G31920 38.889 234 128 5 10 230 13 244 1.50e-43 157
MS.gene053874.t1 AT5G58080 38.618 246 125 5 10 232 14 256 5.01e-42 154
MS.gene053874.t1 AT3G16857 38.839 224 90 3 49 226 2 224 3.57e-41 151
MS.gene053874.t1 AT3G62670 33.929 224 124 6 27 227 52 274 2.75e-33 127
MS.gene053874.t1 AT2G01760 39.378 193 82 5 68 226 1 192 4.58e-33 124
MS.gene053874.t1 AT1G49190 31.020 245 133 6 10 221 30 271 8.85e-31 120
MS.gene053874.t1 AT5G49240 32.068 237 133 6 9 218 37 272 1.19e-29 114
MS.gene053874.t1 AT1G67710 38.503 187 86 4 68 226 1 186 5.48e-29 115
MS.gene053874.t1 AT5G07210 27.675 271 139 7 14 230 16 283 1.09e-21 95.9
MS.gene053874.t1 AT1G67710 37.725 167 75 4 88 226 12 177 1.50e-20 92.0
MS.gene053874.t1 AT5G07210 26.882 279 139 8 14 230 16 291 2.90e-19 88.6
MS.gene053874.t1 AT1G49190 35.714 126 79 2 10 134 30 154 7.76e-18 84.3
MS.gene053874.t1 AT1G68210 25.926 270 142 7 8 221 7 274 2.66e-17 82.8
MS.gene053874.t1 AT5G59570 55.000 60 27 0 167 226 139 198 1.95e-15 75.9
MS.gene053874.t1 AT5G59570 55.000 60 27 0 167 226 139 198 1.95e-15 75.9
MS.gene053874.t1 AT5G05090 57.627 59 25 0 168 226 80 138 2.64e-15 75.1
MS.gene053874.t1 AT3G10760 57.627 59 25 0 168 226 104 162 4.39e-15 75.1
MS.gene053874.t1 AT2G40970 55.932 59 26 0 168 226 104 162 4.48e-15 73.9
MS.gene053874.t1 AT3G46640 49.315 73 34 1 157 226 129 201 4.51e-15 75.1
MS.gene053874.t1 AT3G46640 49.315 73 34 1 157 226 129 201 4.52e-15 75.1
MS.gene053874.t1 AT3G46640 49.315 73 34 1 157 226 129 201 4.52e-15 75.1
MS.gene053874.t1 AT2G27070 25.753 299 158 10 1 242 3 294 5.79e-15 75.9
MS.gene053874.t1 AT3G04030 55.385 65 27 2 158 221 35 98 6.50e-15 75.1
MS.gene053874.t1 AT3G04030 55.385 65 27 2 158 221 35 98 7.47e-15 75.1
MS.gene053874.t1 AT5G18240 55.385 65 27 2 158 221 35 98 1.86e-14 73.9
MS.gene053874.t1 AT5G18240 55.385 65 27 2 158 221 35 98 1.86e-14 73.9
MS.gene053874.t1 AT5G18240 55.385 65 27 2 158 221 35 98 1.88e-14 73.9
MS.gene053874.t1 AT5G18240 55.385 65 27 2 158 221 35 98 1.94e-14 73.6
MS.gene053874.t1 AT5G18240 55.385 65 27 2 158 221 35 98 1.94e-14 73.6
MS.gene053874.t1 AT4G13640 40.741 108 54 3 165 268 33 134 4.31e-14 72.0
MS.gene053874.t1 AT4G13640 40.741 108 54 3 165 268 33 134 4.70e-14 71.6
MS.gene053874.t1 AT3G04030 51.562 64 30 1 158 221 35 97 7.25e-14 72.0
MS.gene053874.t1 AT2G01060 49.412 85 39 3 158 238 4 88 8.91e-14 70.9
MS.gene053874.t1 AT4G04605 42.857 77 42 2 167 241 12 88 1.08e-13 69.7
MS.gene053874.t1 AT5G44190 55.172 58 26 0 168 225 146 203 1.49e-13 70.9
MS.gene053874.t1 AT3G24120 39.450 109 54 4 165 268 37 138 1.74e-13 70.1
MS.gene053874.t1 AT2G40260 47.945 73 37 1 151 222 64 136 1.79e-13 70.9
MS.gene053874.t1 AT5G44190 55.172 58 26 0 168 225 146 203 1.91e-13 70.5
MS.gene053874.t1 AT3G24120 39.450 109 54 4 165 268 37 138 1.95e-13 70.1
MS.gene053874.t1 AT2G20570 46.377 69 37 0 157 225 141 209 3.89e-13 70.1
MS.gene053874.t1 AT5G06800 45.570 79 36 3 169 240 192 270 4.21e-13 69.7
MS.gene053874.t1 AT2G42660 47.692 65 33 1 159 222 40 104 4.55e-13 68.6
MS.gene053874.t1 AT1G14600 59.259 54 21 1 170 222 24 77 4.72e-13 68.2
MS.gene053874.t1 AT5G06800 44.048 84 35 3 169 240 192 275 1.10e-12 68.6
MS.gene053874.t1 AT2G20570 56.364 55 24 0 171 225 171 225 1.59e-12 68.2
MS.gene053874.t1 AT1G69580 36.885 122 66 5 169 284 34 150 2.64e-12 67.0
MS.gene053874.t1 AT2G38300 50.877 57 27 1 167 222 52 108 2.67e-12 67.0
MS.gene053874.t1 AT2G02060 50.000 62 30 1 170 230 30 91 2.69e-12 66.2
MS.gene053874.t1 AT2G02060 50.000 62 30 1 170 230 30 91 2.69e-12 66.2
MS.gene053874.t1 AT5G29000 34.862 109 63 2 129 236 198 299 3.44e-12 66.6
MS.gene053874.t1 AT4G28610 45.946 74 38 2 169 241 225 297 3.44e-12 67.0
MS.gene053874.t1 AT5G24470 29.134 127 85 3 13 135 49 174 4.02e-12 67.0
MS.gene053874.t1 AT5G06800 55.556 54 23 1 169 221 192 245 4.23e-12 65.9
MS.gene053874.t1 AT3G04450 51.515 66 22 2 166 221 226 291 4.46e-12 66.6
MS.gene053874.t1 AT5G29000 31.746 126 78 2 121 245 147 265 4.56e-12 66.6
MS.gene053874.t1 AT5G29000 31.746 126 78 2 121 245 147 265 4.56e-12 66.6
MS.gene053874.t1 AT3G04450 51.515 66 22 2 166 221 186 251 4.97e-12 66.6
MS.gene053874.t1 AT3G04450 51.515 66 22 2 166 221 186 251 4.97e-12 66.6
MS.gene053874.t1 AT5G29000 31.746 126 78 2 121 245 190 308 5.50e-12 66.6
MS.gene053874.t1 AT3G04450 44.304 79 34 2 153 221 213 291 5.64e-12 65.9
MS.gene053874.t1 AT3G12730 51.724 58 27 1 165 221 19 76 6.08e-12 64.7
MS.gene053874.t1 AT1G79430 44.944 89 45 3 165 252 30 115 6.17e-12 66.2
MS.gene053874.t1 AT2G20400 57.407 54 22 1 169 221 231 284 6.24e-12 66.2
MS.gene053874.t1 AT2G20400 57.407 54 22 1 169 221 231 284 6.51e-12 66.2
MS.gene053874.t1 AT1G69580 38.835 103 56 4 169 269 34 131 8.48e-12 65.5
MS.gene053874.t1 AT3G13040 36.752 117 64 5 168 281 240 349 9.23e-12 65.9
MS.gene053874.t1 AT3G13040 36.752 117 64 5 168 281 240 349 9.23e-12 65.9
MS.gene053874.t1 AT3G13040 36.752 117 64 5 168 281 240 349 9.23e-12 65.9
MS.gene053874.t1 AT5G45580 53.448 58 26 1 165 221 3 60 1.81e-11 63.9
MS.gene053874.t1 AT5G45580 49.231 65 32 1 158 221 12 76 1.85e-11 63.9
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 8.84e-11 63.2
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 8.84e-11 63.2
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 8.84e-11 63.2
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 8.84e-11 63.2
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 8.84e-11 63.2
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 9.31e-11 62.8
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 9.31e-11 62.8
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 9.31e-11 62.8
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 9.31e-11 62.8
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 9.31e-11 62.8
MS.gene053874.t1 AT4G18020 45.455 66 35 1 160 225 288 352 9.31e-11 62.8

Find 63 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGGAAGCTTGGATGTTC+AGG 0.252747 5.3:-43879906 MS.gene053874:CDS
CAACAAAATTGGATGGAAAT+TGG 0.274934 5.3:-43879299 MS.gene053874:CDS
CATATGGGGCTTGCGAATAT+TGG 0.282422 5.3:-43880015 MS.gene053874:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 5.3:-43879610 MS.gene053874:intron
AATTCATCAGTTGATTATTT+CGG 0.292540 5.3:-43879209 MS.gene053874:CDS
GCAATAACGTTGAGACCATT+AGG 0.294493 5.3:+43880501 None:intergenic
AAGATGATGCACAACAAAAT+TGG 0.295199 5.3:-43879310 MS.gene053874:CDS
GAATTAATCACGACACAATC+TGG 0.322128 5.3:+43879227 None:intergenic
TTACCAAGTAACCTTGATAA+TGG 0.334363 5.3:+43880424 None:intergenic
TTCTGATGCATTGAACTTGT+TGG 0.353399 5.3:-43880297 MS.gene053874:CDS
GTTACAAAGTCGATCACATA+TGG 0.372078 5.3:-43880031 MS.gene053874:CDS
GAACGTCATCATGTTGGTTC+CGG 0.381973 5.3:+43879375 None:intergenic
ACTTGAAAATATTGGAAGCT+TGG 0.394226 5.3:-43879915 MS.gene053874:CDS
ATCAGAAGCTGTATTGCATT+TGG 0.401294 5.3:+43880313 None:intergenic
ATTGAACTTGTTGGAAAGAA+AGG 0.408638 5.3:-43880288 MS.gene053874:CDS
TTCCGGTGACCATGACAAAC+GGG 0.410735 5.3:+43879823 None:intergenic
CCCGCGGATAACTTTCATCT+TGG 0.418067 5.3:+43879336 None:intergenic
ACATCTCCTCAATGGTGTTA+AGG 0.419121 5.3:+43880457 None:intergenic
CAATAACGTTGAGACCATTA+GGG 0.421209 5.3:+43880502 None:intergenic
TTGAGACCATTAGGGAATGA+TGG 0.453801 5.3:+43880510 None:intergenic
AAGTCATAGGCATCCATGTT+TGG 0.461471 5.3:+43880230 None:intergenic
ATGTTCAGGGTCATAGAAAA+CGG 0.475696 5.3:-43879892 MS.gene053874:CDS
GTTCCGGTGACCATGACAAA+CGG 0.489781 5.3:+43879822 None:intergenic
TTGGACCAAGCCGTTGTATG+AGG 0.493917 5.3:-43879996 MS.gene053874:CDS
GACAACCCATCATTCCCTAA+TGG 0.494012 5.3:-43880516 MS.gene053874:CDS
CTCAATGGTGTTAAGGAAAG+TGG 0.494366 5.3:+43880464 None:intergenic
TAGAAGCTCAGCGGATTGAC+CGG 0.500927 5.3:-43879394 MS.gene053874:CDS
TGGCACCATGTTGTAAGAAA+AGG 0.502846 5.3:-43879956 MS.gene053874:CDS
TGTTCAGGGTCATAGAAAAC+GGG 0.503985 5.3:-43879891 MS.gene053874:CDS
ATTGGAAGCTTGGATGTTCA+GGG 0.504724 5.3:-43879905 MS.gene053874:CDS
TTACAAAGTCGATCACATAT+GGG 0.505649 5.3:-43880030 MS.gene053874:CDS
GCCCCGTTTGTCATGGTCAC+CGG 0.505882 5.3:-43879825 MS.gene053874:CDS
ACACAATCTGGGAACAAGTT+GGG 0.508030 5.3:+43879239 None:intergenic
CTTTGTAACAGAACTTAAAG+TGG 0.517752 5.3:+43880045 None:intergenic
AGGGAATGATGGGTTGTCAA+TGG 0.519036 5.3:+43880521 None:intergenic
AGGAAAGAACCATACTATCA+AGG 0.530499 5.3:+43879762 None:intergenic
TGATGGGTTGTCAATGGAAA+GGG 0.530511 5.3:+43880527 None:intergenic
CACTTTCCTTAACACCATTG+AGG 0.536078 5.3:-43880463 MS.gene053874:CDS
TTGAATTGTTCCTCATACAA+CGG 0.536378 5.3:+43879986 None:intergenic
AGCATGTTGAACAAAGTCAT+AGG 0.540588 5.3:+43880217 None:intergenic
TGTAATCGATGCCATTATCA+AGG 0.541272 5.3:-43880435 MS.gene053874:intron
GACACAATCTGGGAACAAGT+TGG 0.557153 5.3:+43879238 None:intergenic
TGATGCACAACAAAATTGGA+TGG 0.559172 5.3:-43879306 MS.gene053874:CDS
TTTGTAACAGAACTTAAAGT+GGG 0.561171 5.3:+43880046 None:intergenic
TGAGACCATTAGGGAATGAT+GGG 0.561537 5.3:+43880511 None:intergenic
TCGATTACACATCTCCTCAA+TGG 0.564409 5.3:+43880449 None:intergenic
TTATGGGCTATCAATCAACT+TGG 0.565523 5.3:-43879785 MS.gene053874:CDS
AATTAATCACGACACAATCT+GGG 0.566059 5.3:+43879228 None:intergenic
CGAAGAAGCCCCGTTTGTCA+TGG 0.567178 5.3:-43879832 MS.gene053874:CDS
GATGTTCATGGTTTGAGTGT+AGG 0.572619 5.3:-43879633 MS.gene053874:CDS
ATGATGACGTTCAAGATGAG+TGG 0.593959 5.3:-43879364 MS.gene053874:CDS
TTGTTCCTCATACAACGGCT+TGG 0.596667 5.3:+43879991 None:intergenic
CATCTTGAACGTCATCATGT+TGG 0.599748 5.3:+43879369 None:intergenic
ATGATGGGTTGTCAATGGAA+AGG 0.601102 5.3:+43880526 None:intergenic
CCAAGATGAAAGTTATCCGC+GGG 0.608633 5.3:-43879336 MS.gene053874:CDS
TCTTACAACATGGTGCCACA+TGG 0.626414 5.3:+43879961 None:intergenic
TGTGCATCATCTTGTACCCG+CGG 0.630231 5.3:+43879320 None:intergenic
TGATGTTCATATGCCAAACA+TGG 0.637365 5.3:-43880243 MS.gene053874:CDS
ACCAAGATGAAAGTTATCCG+CGG 0.659940 5.3:-43879337 MS.gene053874:CDS
TACAAAGTCGATCACATATG+GGG 0.662060 5.3:-43880029 MS.gene053874:CDS
TCCGGTGACCATGACAAACG+GGG 0.701794 5.3:+43879824 None:intergenic
AGGAACAATTCAAGACCATG+TGG 0.731329 5.3:-43879976 MS.gene053874:CDS
GTGATGACTTAGAAGCTCAG+CGG 0.758152 5.3:-43879403 MS.gene053874:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAACTTGAAAATAT+TGG - chr5.3:43879813-43879832 MS.gene053874:CDS 10.0%
!! GAGTTTATTATAATTGTAAT+TGG - chr5.3:43880226-43880245 MS.gene053874:CDS 15.0%
!! AAGAAAAACTTACTAATAAC+AGG + chr5.3:43879563-43879582 None:intergenic 20.0%
!! AATTCATCAGTTGATTATTT+CGG - chr5.3:43880527-43880546 MS.gene053874:CDS 20.0%
!! TGTTTGTATTATTTAACAAC+TGG - chr5.3:43880014-43880033 MS.gene053874:CDS 20.0%
!! TTATAATTGTAATTGGAATG+TGG - chr5.3:43880233-43880252 MS.gene053874:CDS 20.0%
! AAAAAAGTTGCGAATATTGT+CGG + chr5.3:43880468-43880487 None:intergenic 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG + chr5.3:43879997-43880016 None:intergenic 25.0%
! TTAAAATCACACAAAACATG+TGG + chr5.3:43880193-43880212 None:intergenic 25.0%
! TTTGTAACAGAACTTAAAGT+GGG + chr5.3:43879693-43879712 None:intergenic 25.0%
!! TAAACTGATTCTCAAAAAGA+TGG - chr5.3:43880081-43880100 MS.gene053874:intron 25.0%
!!! AACTCTAGAATTGATGAATA+AGG + chr5.3:43879344-43879363 None:intergenic 25.0%
!!! ATCTTTTTGAGAATCAGTTT+AGG + chr5.3:43880082-43880101 None:intergenic 25.0%
AAGATGATGCACAACAAAAT+TGG - chr5.3:43880426-43880445 MS.gene053874:intron 30.0%
AATTAATCACGACACAATCT+GGG + chr5.3:43880511-43880530 None:intergenic 30.0%
ACTTGAAAATATTGGAAGCT+TGG - chr5.3:43879821-43879840 MS.gene053874:CDS 30.0%
CAACAAAATTGGATGGAAAT+TGG - chr5.3:43880437-43880456 MS.gene053874:CDS 30.0%
CGATTGATGAGAAAAAATGT+GGG - chr5.3:43880291-43880310 MS.gene053874:CDS 30.0%
CTTTGTAACAGAACTTAAAG+TGG + chr5.3:43879694-43879713 None:intergenic 30.0%
TCGATTGATGAGAAAAAATG+TGG - chr5.3:43880290-43880309 MS.gene053874:CDS 30.0%
TTACAAAGTCGATCACATAT+GGG - chr5.3:43879706-43879725 MS.gene053874:intron 30.0%
TTACCAAGTAACCTTGATAA+TGG + chr5.3:43879315-43879334 None:intergenic 30.0%
TTGAATTGTTCCTCATACAA+CGG + chr5.3:43879753-43879772 None:intergenic 30.0%
! AACTGCATCAACGATTTTTA+TGG - chr5.3:43879934-43879953 MS.gene053874:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG - chr5.3:43879935-43879954 MS.gene053874:CDS 30.0%
!! ATTGAACTTGTTGGAAAGAA+AGG - chr5.3:43879448-43879467 MS.gene053874:CDS 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG - chr5.3:43880051-43880070 MS.gene053874:CDS 30.0%
AAAAATCGTTGATGCAGTTC+CGG + chr5.3:43879933-43879952 None:intergenic 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr5.3:43880126-43880145 MS.gene053874:intron 35.0%
AGCATGTTGAACAAAGTCAT+AGG + chr5.3:43879522-43879541 None:intergenic 35.0%
AGGAAAGAACCATACTATCA+AGG + chr5.3:43879977-43879996 None:intergenic 35.0%
ATCAGAAGCTGTATTGCATT+TGG + chr5.3:43879426-43879445 None:intergenic 35.0%
ATGCCATTATCAAGGTTACT+TGG - chr5.3:43879309-43879328 MS.gene053874:CDS 35.0%
ATGTTCAGGGTCATAGAAAA+CGG - chr5.3:43879844-43879863 MS.gene053874:CDS 35.0%
ATTCAGTAGACATCACTACA+AGG + chr5.3:43879668-43879687 None:intergenic 35.0%
CAATAACGTTGAGACCATTA+GGG + chr5.3:43879237-43879256 None:intergenic 35.0%
CTCAAAAAGATGGATGTTCA+TGG - chr5.3:43880091-43880110 MS.gene053874:intron 35.0%
GAATTAATCACGACACAATC+TGG + chr5.3:43880512-43880531 None:intergenic 35.0%
GTTACAAAGTCGATCACATA+TGG - chr5.3:43879705-43879724 MS.gene053874:intron 35.0%
TACAAAGTCGATCACATATG+GGG - chr5.3:43879707-43879726 MS.gene053874:intron 35.0%
TGATGCACAACAAAATTGGA+TGG - chr5.3:43880430-43880449 MS.gene053874:intron 35.0%
TGATGTTCATATGCCAAACA+TGG - chr5.3:43879493-43879512 MS.gene053874:intron 35.0%
TGTAATCGATGCCATTATCA+AGG - chr5.3:43879301-43879320 MS.gene053874:CDS 35.0%
TTCTGATGCATTGAACTTGT+TGG - chr5.3:43879439-43879458 MS.gene053874:CDS 35.0%
! TCAGACCTTTTCTTACAACA+TGG + chr5.3:43879788-43879807 None:intergenic 35.0%
! TTATGGGCTATCAATCAACT+TGG - chr5.3:43879951-43879970 MS.gene053874:CDS 35.0%
AAGTCATAGGCATCCATGTT+TGG + chr5.3:43879509-43879528 None:intergenic 40.0%
ACACAATCTGGGAACAAGTT+GGG + chr5.3:43880500-43880519 None:intergenic 40.0%
ACCAAGATGAAAGTTATCCG+CGG - chr5.3:43880399-43880418 MS.gene053874:intron 40.0%
AGGAACAATTCAAGACCATG+TGG - chr5.3:43879760-43879779 MS.gene053874:intron 40.0%
ATTGGAAGCTTGGATGTTCA+GGG - chr5.3:43879831-43879850 MS.gene053874:CDS 40.0%
CACTTTCCTTAACACCATTG+AGG - chr5.3:43879273-43879292 MS.gene053874:CDS 40.0%
CATCTTGAACGTCATCATGT+TGG + chr5.3:43880370-43880389 None:intergenic 40.0%
GATGTTCATGGTTTGAGTGT+AGG - chr5.3:43880103-43880122 MS.gene053874:intron 40.0%
GCAATAACGTTGAGACCATT+AGG + chr5.3:43879238-43879257 None:intergenic 40.0%
TATTGGAAGCTTGGATGTTC+AGG - chr5.3:43879830-43879849 MS.gene053874:CDS 40.0%
TCGATTACACATCTCCTCAA+TGG + chr5.3:43879290-43879309 None:intergenic 40.0%
TGAGACCATTAGGGAATGAT+GGG + chr5.3:43879228-43879247 None:intergenic 40.0%
TGGCACCATGTTGTAAGAAA+AGG - chr5.3:43879780-43879799 MS.gene053874:CDS 40.0%
TGTTCAGGGTCATAGAAAAC+GGG - chr5.3:43879845-43879864 MS.gene053874:CDS 40.0%
TTGAGACCATTAGGGAATGA+TGG + chr5.3:43879229-43879248 None:intergenic 40.0%
! ACATCTCCTCAATGGTGTTA+AGG + chr5.3:43879282-43879301 None:intergenic 40.0%
! ATGATGACGTTCAAGATGAG+TGG - chr5.3:43880372-43880391 MS.gene053874:intron 40.0%
! CTCAATGGTGTTAAGGAAAG+TGG + chr5.3:43879275-43879294 None:intergenic 40.0%
!! ATGATGGGTTGTCAATGGAA+AGG + chr5.3:43879213-43879232 None:intergenic 40.0%
!! TCAACTTGGCCTTGATAGTA+TGG - chr5.3:43879965-43879984 MS.gene053874:CDS 40.0%
!! TGATGGGTTGTCAATGGAAA+GGG + chr5.3:43879212-43879231 None:intergenic 40.0%
CATATGGGGCTTGCGAATAT+TGG - chr5.3:43879721-43879740 MS.gene053874:intron 45.0%
CCAAGATGAAAGTTATCCGC+GGG - chr5.3:43880400-43880419 MS.gene053874:intron 45.0%
GACAACCCATCATTCCCTAA+TGG - chr5.3:43879220-43879239 MS.gene053874:CDS 45.0%
GACACAATCTGGGAACAAGT+TGG + chr5.3:43880501-43880520 None:intergenic 45.0%
GTGATGACTTAGAAGCTCAG+CGG - chr5.3:43880333-43880352 MS.gene053874:intron 45.0%
TTGTTCCTCATACAACGGCT+TGG + chr5.3:43879748-43879767 None:intergenic 45.0%
!! AGGGAATGATGGGTTGTCAA+TGG + chr5.3:43879218-43879237 None:intergenic 45.0%
!! GAACGTCATCATGTTGGTTC+CGG + chr5.3:43880364-43880383 None:intergenic 45.0%
!! TCTTACAACATGGTGCCACA+TGG + chr5.3:43879778-43879797 None:intergenic 45.0%
!!! TGATTTTAAATTTAAATTTA+TGG - chr5.3:43880204-43880223 MS.gene053874:CDS 5.0%
CCCGCGGATAACTTTCATCT+TGG + chr5.3:43880403-43880422 None:intergenic 50.0%
GTTCCGGTGACCATGACAAA+CGG + chr5.3:43879917-43879936 None:intergenic 50.0%
TGTGCATCATCTTGTACCCG+CGG + chr5.3:43880419-43880438 None:intergenic 50.0%
TTCCGGTGACCATGACAAAC+GGG + chr5.3:43879916-43879935 None:intergenic 50.0%
TTGGACCAAGCCGTTGTATG+AGG - chr5.3:43879740-43879759 MS.gene053874:intron 50.0%
!! TAGAAGCTCAGCGGATTGAC+CGG - chr5.3:43880342-43880361 MS.gene053874:intron 50.0%
CGAAGAAGCCCCGTTTGTCA+TGG - chr5.3:43879904-43879923 MS.gene053874:CDS 55.0%
TCCGGTGACCATGACAAACG+GGG + chr5.3:43879915-43879934 None:intergenic 55.0%
GCCCCGTTTGTCATGGTCAC+CGG - chr5.3:43879911-43879930 MS.gene053874:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 43879205 43880553 43879205 ID=MS.gene053874
chr5.3 mRNA 43879205 43880553 43879205 ID=MS.gene053874.t1;Parent=MS.gene053874
chr5.3 exon 43880436 43880553 43880436 ID=MS.gene053874.t1.exon1;Parent=MS.gene053874.t1
chr5.3 CDS 43880436 43880553 43880436 ID=cds.MS.gene053874.t1;Parent=MS.gene053874.t1
chr5.3 exon 43880189 43880338 43880189 ID=MS.gene053874.t1.exon2;Parent=MS.gene053874.t1
chr5.3 CDS 43880189 43880338 43880189 ID=cds.MS.gene053874.t1;Parent=MS.gene053874.t1
chr5.3 exon 43879777 43880085 43879777 ID=MS.gene053874.t1.exon3;Parent=MS.gene053874.t1
chr5.3 CDS 43879777 43880085 43879777 ID=cds.MS.gene053874.t1;Parent=MS.gene053874.t1
chr5.3 exon 43879611 43879687 43879611 ID=MS.gene053874.t1.exon4;Parent=MS.gene053874.t1
chr5.3 CDS 43879611 43879687 43879611 ID=cds.MS.gene053874.t1;Parent=MS.gene053874.t1
chr5.3 exon 43879205 43879492 43879205 ID=MS.gene053874.t1.exon5;Parent=MS.gene053874.t1
chr5.3 CDS 43879205 43879492 43879205 ID=cds.MS.gene053874.t1;Parent=MS.gene053874.t1
Gene Sequence

>MS.gene053874

ATGGCCCTTTCCATTGACAACCCATCATTCCCTAATGGTCTCAACGTTATTGCAATTGATCATGACACCACTTTCCTTAACACCATTGAGGAGATGTGTAATCGATGCCATTATCAAGGTTACTTGGTAAATTACACCTTATTCATCAATTCTAGAGTTTTCATCTTTTTCTAAAAATTCTTGATTTTACATATTTTTACGTTTGTTTATTTCAGTTACCAAATGCAATACAGCTTCTGATGCATTGAACTTGTTGGAAAGAAAGGACTGTTTTGATGTAATGTTAATTGATGTTCATATGCCAAACATGGATGCCTATGACTTTGTTCAACATGCTACAGTACAACTCAATATTCCTGTTATTAGTAAGTTTTTCTTCATCTTTATACTTATACTTTGTTACATTCATTCAAATATATATACTTATGAAGTGTGAGTGTGACTGACATTGATATTTCTTCCTTGTAGTGATGTCTACTGAATATCCCACTTTAAGTTCTGTTACAAAGTCGATCACATATGGGGCTTGCGAATATTGGACCAAGCCGTTGTATGAGGAACAATTCAAGACCATGTGGCACCATGTTGTAAGAAAAGGTCTGAGCGAAAATAAAAAACTTGAAAATATTGGAAGCTTGGATGTTCAGGGTCATAGAAAACGGGAAAGAGAAGATGATAATGTCACAAAAGAAACAAATGCGAAGAAGCCCCGTTTGTCATGGTCACCGGAACTGCATCAACGATTTTTATGGGCTATCAATCAACTTGGCCTTGATAGTATGGTTCTTTCCTAATTCTTTTCTTTGCTTTGTTTGTATTATTTAACAACTGGACTAATGTGCATTTTTCTCTCACTTATTTTTCAGAGGCAAAACCTAAACTGATTCTCAAAAAGATGGATGTTCATGGTTTGAGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTTTAATAGAATCTAAGAAAATTATTGATAATATACCACATGTTTTGTGTGATTTTAAATTTAAATTTATGGAGTTTATTATAATTGTAATTGGAATGTGGTAATTTGCAGAAATATAGAAACTATTTGAAGAGATCGATTGATGAGAAAAAATGTGGGAAGAAAAAATCAAAGCTGAGTGATGACTTAGAAGCTCAGCGGATTGACCGGAACCAACATGATGACGTTCAAGATGAGTGGATTGACCAAGATGAAAGTTATCCGCGGGTACAAGATGATGCACAACAAAATTGGATGGAAATTGGCAAATCCGACAATATTCGCAACTTTTTTTCTGACGTACCCAACTTGTTCCCAGATTGTGTCGTGATTAATTCATCAGTTGATTATTTCGGCTAG
Protein sequence

>MS.gene053874.t1

MALSIDNPSFPNGLNVIAIDHDTTFLNTIEEMCNRCHYQVTKCNTASDALNLLERKDCFDVMLIDVHMPNMDAYDFVQHATVQLNIPVIMMSTEYPTLSSVTKSITYGACEYWTKPLYEEQFKTMWHHVVRKGLSENKKLENIGSLDVQGHRKREREDDNVTKETNAKKPRLSWSPELHQRFLWAINQLGLDKAKPKLILKKMDVHGLSVGHVASHLQKYRNYLKRSIDEKKCGKKKSKLSDDLEAQRIDRNQHDDVQDEWIDQDESYPRVQDDAQQNWMEIGKSDNIRNFFSDVPNLFPDCVVINSSVDYFG