AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054152

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054152.t1 MTR_1g029100 84.444 90 14 0 42 131 1 90 4.80e-52 160
MS.gene054152.t1 MTR_1g029070 42.857 140 71 2 1 131 1 140 9.38e-31 109
MS.gene054152.t1 MTR_4g133952 38.525 122 67 2 16 132 29 147 2.93e-23 91.7
MS.gene054152.t1 MTR_1g039040 40.179 112 56 2 29 140 2 102 7.82e-22 86.7
MS.gene054152.t1 MTR_1g072790 40.594 101 50 1 48 138 34 134 8.55e-22 86.3
MS.gene054152.t1 MTR_3g058980 40.404 99 58 1 48 146 32 129 1.41e-21 86.3
MS.gene054152.t1 MTR_7g061270 38.318 107 63 1 48 151 31 137 3.65e-21 84.3
MS.gene054152.t1 MTR_7g100650 38.318 107 63 1 48 151 31 137 4.75e-21 84.3
MS.gene054152.t1 MTR_1g088860 42.353 85 49 0 48 132 41 125 7.20e-21 84.3
MS.gene054152.t1 MTR_8g091720 40.594 101 59 1 48 148 33 132 8.00e-21 84.3
MS.gene054152.t1 MTR_5g095740 43.529 85 48 0 48 132 28 112 1.16e-20 83.6
MS.gene054152.t1 MTR_2g026710 42.697 89 50 1 48 136 22 109 1.58e-20 82.8
MS.gene054152.t1 MTR_4g133938 40.659 91 54 0 42 132 18 108 1.94e-20 81.6
MS.gene054152.t1 MTR_0392s0020 34.545 110 62 1 23 132 8 107 4.43e-20 80.9
MS.gene054152.t1 MTR_4g119500 38.824 85 52 0 48 132 27 111 3.14e-19 78.6
MS.gene054152.t1 MTR_8g093920 42.857 84 48 0 48 131 45 128 5.10e-19 80.1
MS.gene054152.t1 MTR_1g083070 35.294 85 55 0 48 132 9 93 2.43e-18 76.3
MS.gene054152.t1 MTR_5g095900 36.905 84 53 0 48 131 21 104 3.59e-15 69.3
MS.gene054152.t1 MTR_1g028480 38.372 86 39 1 79 150 25 110 1.39e-14 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054152.t1 AT2G38880 42.574 101 57 1 48 147 26 126 3.52e-23 89.0
MS.gene054152.t1 AT2G38880 41.748 103 57 1 48 147 26 128 6.90e-23 88.2
MS.gene054152.t1 AT2G38880 41.748 103 57 1 48 147 26 128 7.72e-23 88.2
MS.gene054152.t1 AT2G38880 41.748 103 57 1 48 147 26 128 7.72e-23 88.2
MS.gene054152.t1 AT2G38880 41.748 103 57 1 48 147 26 128 7.72e-23 88.2
MS.gene054152.t1 AT2G37060 42.453 106 58 1 48 150 35 140 1.01e-22 88.6
MS.gene054152.t1 AT2G37060 42.453 106 58 1 48 150 35 140 1.01e-22 88.6
MS.gene054152.t1 AT2G37060 42.453 106 58 1 48 150 35 140 1.01e-22 88.6
MS.gene054152.t1 AT3G53340 42.453 106 58 1 48 150 34 139 1.17e-22 88.6
MS.gene054152.t1 AT3G53340 42.453 106 58 1 48 150 34 139 1.17e-22 88.6
MS.gene054152.t1 AT1G21970 33.108 148 83 1 1 132 1 148 1.82e-21 87.0
MS.gene054152.t1 AT4G14540 37.963 108 66 1 25 132 4 110 2.70e-21 84.7
MS.gene054152.t1 AT2G38880 43.529 85 48 0 48 132 26 110 2.86e-21 83.2
MS.gene054152.t1 AT2G38880 43.529 85 48 0 48 132 26 110 8.76e-21 83.6
MS.gene054152.t1 AT2G38880 43.529 85 48 0 48 132 26 110 8.76e-21 83.6
MS.gene054152.t1 AT5G47640 44.706 85 47 0 48 132 32 116 9.64e-21 84.0
MS.gene054152.t1 AT5G47670 38.947 95 58 0 48 142 34 128 1.58e-20 84.0
MS.gene054152.t1 AT2G47810 40.385 104 59 2 31 131 36 139 1.67e-20 82.8
MS.gene054152.t1 AT2G38880 43.529 85 48 0 48 132 26 110 1.85e-20 82.4
MS.gene054152.t1 AT2G38880 43.529 85 48 0 48 132 26 110 1.85e-20 82.4
MS.gene054152.t1 AT2G38880 43.529 85 48 0 48 132 26 110 1.85e-20 82.4
MS.gene054152.t1 AT5G47670 38.947 95 58 0 48 142 63 157 3.11e-20 83.6
MS.gene054152.t1 AT5G47670 38.947 95 58 0 48 142 63 157 3.11e-20 83.6
MS.gene054152.t1 AT3G53340 44.706 85 47 0 48 132 34 118 6.63e-20 80.1
MS.gene054152.t1 AT3G53340 44.706 85 47 0 48 132 34 118 1.22e-18 77.4
MS.gene054152.t1 AT3G53340 44.706 85 47 0 48 132 34 118 1.22e-18 77.4
MS.gene054152.t1 AT2G13570 42.857 84 48 0 48 131 41 124 3.13e-18 78.2
MS.gene054152.t1 AT1G09030 33.696 92 60 1 42 132 1 92 2.40e-16 71.2

Find 38 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCACTTCCTCGGCCATTGC+TGG 0.302846 1.3:-15114215 None:intergenic
TTTCTGCTGATGATTTACTA+TGG 0.312242 1.3:+15114402 MS.gene054152:CDS
GAGGAATCTGTTCGGTCTCT+TGG 0.334557 1.3:+15114446 MS.gene054152:CDS
ATTTACTATGGACGATGAAT+AGG 0.363355 1.3:+15114414 MS.gene054152:CDS
CGCAAGAACATGGATGGTTC+AGG 0.394145 1.3:+15114488 MS.gene054152:CDS
AGGAATCTGTTCGGTCTCTT+GGG 0.418982 1.3:+15114447 MS.gene054152:CDS
GATGAATAGGTTGGGCTTTG+AGG 0.427694 1.3:+15114427 MS.gene054152:CDS
CGTTCGGCACACTCAAGCTT+TGG 0.430626 1.3:+15113083 MS.gene054152:CDS
GATGTTCTACATTGGCTTGT+TGG 0.438115 1.3:-15114370 None:intergenic
CTATGGACGATGAATAGGTT+GGG 0.452990 1.3:+15114419 MS.gene054152:CDS
ACCAACAACAACACTGGCAT+TGG 0.458777 1.3:+15114170 MS.gene054152:CDS
GCCAGTGTTGTTGTTGGTGG+TGG 0.460032 1.3:-15114165 None:intergenic
TCCAATGCCAGTGTTGTTGT+TGG 0.470529 1.3:-15114171 None:intergenic
TCAACGTTGGTCATCATCAT+TGG 0.472149 1.3:-15114531 None:intergenic
GAACGGTTGTTGTTAGATGA+AGG 0.489970 1.3:-15113065 None:intergenic
AATGCCAGTGTTGTTGTTGG+TGG 0.494945 1.3:-15114168 None:intergenic
GACTCTTGTCACATGACTGA+TGG 0.498388 1.3:-15114243 None:intergenic
TGGGCTTTGAGGAATCTGTT+CGG 0.518806 1.3:+15114438 MS.gene054152:CDS
TTCATCTAACAACAACCGTT+CGG 0.526767 1.3:+15113067 MS.gene054152:CDS
TCATCAGTGATTATTGTGTC+TGG 0.528332 1.3:-15114284 None:intergenic
ACTATGGACGATGAATAGGT+TGG 0.532041 1.3:+15114418 MS.gene054152:CDS
CCATTGCTGGAGTTGAATGT+TGG 0.538932 1.3:-15114202 None:intergenic
GATGGGCATACGCACTTCCT+CGG 0.539534 1.3:-15114225 None:intergenic
TTCAACTCCAGCAATGGCCG+AGG 0.555161 1.3:+15114208 MS.gene054152:CDS
AGTCATTTGATGTTCTACAT+TGG 0.556175 1.3:-15114378 None:intergenic
AATAATCACTGATGATGCTA+AGG 0.557692 1.3:+15114292 MS.gene054152:CDS
ACTCTTGTCACATGACTGAT+GGG 0.557969 1.3:-15114242 None:intergenic
AACTATCGCAAGAACATGGA+TGG 0.564685 1.3:+15114482 MS.gene054152:CDS
TGTGTCTAAATTTATGAACA+AGG 0.573477 1.3:+15114331 MS.gene054152:CDS
ATGGATGGTTCAGGTGCTAG+TGG 0.576300 1.3:+15114497 MS.gene054152:CDS
ACCACCACCAACAACAACAC+TGG 0.591133 1.3:+15114164 MS.gene054152:CDS
CAACACTGGCATTGGAACTG+AGG 0.595180 1.3:+15114178 MS.gene054152:CDS
CCAACATTCAACTCCAGCAA+TGG 0.604613 1.3:+15114202 MS.gene054152:CDS
CCATAATTTCATATCAACGT+TGG 0.610658 1.3:-15114544 None:intergenic
TATGAACAAGGTCACAAGTG+AGG 0.614031 1.3:+15114343 MS.gene054152:CDS
TCAGTGATTATTGTGTCTGG+TGG 0.634070 1.3:-15114281 None:intergenic
TTCTAAAGCAGTATGGAGCG+TGG 0.645240 1.3:+15113038 None:intergenic
CAAAGCTTGAGTGTGCCGAA+CGG 0.668304 1.3:-15113082 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAAATTAATTAATTA+AGG + chr1.3:15113686-15113705 MS.gene054152:intron 0.0%
!! TAATAAATTAAAAATATTAA+AGG - chr1.3:15113485-15113504 None:intergenic 0.0%
!! TTATTATAATTAATATTATA+TGG - chr1.3:15113671-15113690 None:intergenic 0.0%
!! ATTAAAAATAATAAAATACG+AGG - chr1.3:15113168-15113187 None:intergenic 10.0%
!! AAATTAAGTAAAAGAGATAG+AGG - chr1.3:15113454-15113473 None:intergenic 20.0%
!!! ACTAAGATACAAACATATTT+TGG - chr1.3:15113867-15113886 None:intergenic 20.0%
!!! ATCTTGGAATTAATTTTGTT+CGG + chr1.3:15113572-15113591 MS.gene054152:intron 20.0%
!!! CTTTTCTTCTTTTTAATCTA+AGG - chr1.3:15113983-15114002 None:intergenic 20.0%
!!! TACCAAATCTATTAATTTTG+AGG + chr1.3:15114074-15114093 MS.gene054152:intron 20.0%
!!! TGAATGAAATTTTGATTGAA+GGG + chr1.3:15113396-15113415 MS.gene054152:intron 20.0%
!!! TTGAATGAAATTTTGATTGA+AGG + chr1.3:15113395-15113414 MS.gene054152:intron 20.0%
! AACAAAACTGACGTATTTAA+AGG - chr1.3:15113785-15113804 None:intergenic 25.0%
! AAGAAATAAAGTGAGAGAAA+AGG + chr1.3:15113736-15113755 MS.gene054152:intron 25.0%
! AATCTAATAGAGCAATTAAG+GGG - chr1.3:15113294-15113313 None:intergenic 25.0%
! ACAAAACTGACGTATTTAAA+GGG - chr1.3:15113784-15113803 None:intergenic 25.0%
! ACTTTGTAAAACATTGAATC+CGG + chr1.3:15113195-15113214 MS.gene054152:intron 25.0%
! AGAATCTAATAGAGCAATTA+AGG - chr1.3:15113296-15113315 None:intergenic 25.0%
! ATATAATTGTGCATCACTTA+TGG + chr1.3:15113357-15113376 MS.gene054152:intron 25.0%
! CTCCTCAAAATTAATAGATT+TGG - chr1.3:15114079-15114098 None:intergenic 25.0%
! GAATCTAATAGAGCAATTAA+GGG - chr1.3:15113295-15113314 None:intergenic 25.0%
! TATAATTGTGCATCACTTAT+GGG + chr1.3:15113358-15113377 MS.gene054152:intron 25.0%
! TCTACTATGAAATTAGATAC+CGG - chr1.3:15113217-15113236 None:intergenic 25.0%
! TGTGTCTAAATTTATGAACA+AGG + chr1.3:15114331-15114350 MS.gene054152:CDS 25.0%
!! CTAAAAAATGTTTGAGATCT+TGG + chr1.3:15113556-15113575 MS.gene054152:intron 25.0%
!! GTGTGAGTGTTATAAATTTT+GGG + chr1.3:15113514-15113533 MS.gene054152:intron 25.0%
!! TGTGAGTGTTATAAATTTTG+GGG + chr1.3:15113515-15113534 MS.gene054152:intron 25.0%
!! TGTGTGAGTGTTATAAATTT+TGG + chr1.3:15113513-15113532 MS.gene054152:intron 25.0%
!! TTCTTCTTTTTAATCTAAGG+TGG - chr1.3:15113980-15113999 None:intergenic 25.0%
!!! AGCTAAGATGTTTTTAATAG+AGG - chr1.3:15114135-15114154 None:intergenic 25.0%
!!! GAATGAAATTTTGATTGAAG+GGG + chr1.3:15113397-15113416 MS.gene054152:intron 25.0%
AAAGAGATAGAGGTCAAATA+TGG - chr1.3:15113444-15113463 None:intergenic 30.0%
AAATAAAGTGAGAGAAAAGG+TGG + chr1.3:15113739-15113758 MS.gene054152:intron 30.0%
AAGAGATAGAGGTCAAATAT+GGG - chr1.3:15113443-15113462 None:intergenic 30.0%
AATAAAGTGAGAGAAAAGGT+GGG + chr1.3:15113740-15113759 MS.gene054152:intron 30.0%
AGTCATTTGATGTTCTACAT+TGG - chr1.3:15114381-15114400 None:intergenic 30.0%
ATTGCTCTATTAGATTCTCA+AGG + chr1.3:15113298-15113317 MS.gene054152:intron 30.0%
ATTTACTATGGACGATGAAT+AGG + chr1.3:15114414-15114433 MS.gene054152:CDS 30.0%
CAAAACTGACGTATTTAAAG+GGG - chr1.3:15113783-15113802 None:intergenic 30.0%
GGTGGGTGAAAAAATAAATA+AGG + chr1.3:15113757-15113776 MS.gene054152:intron 30.0%
TTTCTGCTGATGATTTACTA+TGG + chr1.3:15114402-15114421 MS.gene054152:CDS 30.0%
! AATAATCACTGATGATGCTA+AGG + chr1.3:15114292-15114311 MS.gene054152:CDS 30.0%
CAAAAACTATCGCAAGAACA+TGG + chr1.3:15114478-15114497 MS.gene054152:CDS 35.0%
TCATCAGTGATTATTGTGTC+TGG - chr1.3:15114287-15114306 None:intergenic 35.0%
TTCATCTAACAACAACCGTT+CGG + chr1.3:15113067-15113086 MS.gene054152:CDS 35.0%
TTGGTTGTCTGTGTGTATAT+TGG - chr1.3:15113848-15113867 None:intergenic 35.0%
! TGTAGTCACATTTTACAAGC+TGG + chr1.3:15113950-15113969 MS.gene054152:intron 35.0%
! TGTTCTTGCGATAGTTTTTG+AGG - chr1.3:15114478-15114497 None:intergenic 35.0%
AACTATCGCAAGAACATGGA+TGG + chr1.3:15114482-15114501 MS.gene054152:CDS 40.0%
ACTATGGACGATGAATAGGT+TGG + chr1.3:15114418-15114437 MS.gene054152:CDS 40.0%
ACTCTTGTCACATGACTGAT+GGG - chr1.3:15114245-15114264 None:intergenic 40.0%
CTATGGACGATGAATAGGTT+GGG + chr1.3:15114419-15114438 MS.gene054152:CDS 40.0%
GGGCAAGAATGATCTAACTT+GGG + chr1.3:15113334-15113353 MS.gene054152:intron 40.0%
TATGAACAAGGTCACAAGTG+AGG + chr1.3:15114343-15114362 MS.gene054152:CDS 40.0%
TCAACGTTGGTCATCATCAT+TGG - chr1.3:15114534-15114553 None:intergenic 40.0%
TCAGTGATTATTGTGTCTGG+TGG - chr1.3:15114284-15114303 None:intergenic 40.0%
! GAACGGTTGTTGTTAGATGA+AGG - chr1.3:15113068-15113087 None:intergenic 40.0%
! TCTCAAGGCATTATTAGCCT+CGG + chr1.3:15113313-15113332 MS.gene054152:intron 40.0%
!! GATGTTCTACATTGGCTTGT+TGG - chr1.3:15114373-15114392 None:intergenic 40.0%
ACCAACAACAACACTGGCAT+TGG + chr1.3:15114170-15114189 MS.gene054152:CDS 45.0%
AGGAATCTGTTCGGTCTCTT+GGG + chr1.3:15114447-15114466 MS.gene054152:CDS 45.0%
AGTTAGATCATTCTTGCCCG+AGG - chr1.3:15113333-15113352 None:intergenic 45.0%
CCAACATTCAACTCCAGCAA+TGG + chr1.3:15114202-15114221 MS.gene054152:CDS 45.0%
CCATTGCTGGAGTTGAATGT+TGG - chr1.3:15114205-15114224 None:intergenic 45.0%
CGGGCAAGAATGATCTAACT+TGG + chr1.3:15113333-15113352 MS.gene054152:intron 45.0%
GACTCTTGTCACATGACTGA+TGG - chr1.3:15114246-15114265 None:intergenic 45.0%
! AATGCCAGTGTTGTTGTTGG+TGG - chr1.3:15114171-15114190 None:intergenic 45.0%
! CTCAAGGCATTATTAGCCTC+GGG + chr1.3:15113314-15113333 MS.gene054152:intron 45.0%
! GATGAATAGGTTGGGCTTTG+AGG + chr1.3:15114427-15114446 MS.gene054152:CDS 45.0%
! TCCAATGCCAGTGTTGTTGT+TGG - chr1.3:15114174-15114193 None:intergenic 45.0%
!! TGGGCTTTGAGGAATCTGTT+CGG + chr1.3:15114438-15114457 MS.gene054152:CDS 45.0%
ACCACCACCAACAACAACAC+TGG + chr1.3:15114164-15114183 MS.gene054152:CDS 50.0%
ATGGATGGTTCAGGTGCTAG+TGG + chr1.3:15114497-15114516 MS.gene054152:CDS 50.0%
CAAAGCTTGAGTGTGCCGAA+CGG - chr1.3:15113085-15113104 None:intergenic 50.0%
CGCAAGAACATGGATGGTTC+AGG + chr1.3:15114488-15114507 MS.gene054152:CDS 50.0%
GAGCAGATCTGCGTATGCAA+TGG + chr1.3:15113594-15113613 MS.gene054152:intron 50.0%
GAGGAATCTGTTCGGTCTCT+TGG + chr1.3:15114446-15114465 MS.gene054152:CDS 50.0%
! CAACACTGGCATTGGAACTG+AGG + chr1.3:15114178-15114197 MS.gene054152:CDS 50.0%
CGTTCGGCACACTCAAGCTT+TGG + chr1.3:15113083-15113102 MS.gene054152:CDS 55.0%
GATGGGCATACGCACTTCCT+CGG - chr1.3:15114228-15114247 None:intergenic 55.0%
TTCAACTCCAGCAATGGCCG+AGG + chr1.3:15114208-15114227 MS.gene054152:CDS 55.0%
! GCCAGTGTTGTTGTTGGTGG+TGG - chr1.3:15114168-15114187 None:intergenic 55.0%
CGCACTTCCTCGGCCATTGC+TGG - chr1.3:15114218-15114237 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 15113050 15114553 15113050 ID=MS.gene054152
chr1.3 mRNA 15113050 15114553 15113050 ID=MS.gene054152.t1;Parent=MS.gene054152
chr1.3 exon 15113050 15113104 15113050 ID=MS.gene054152.t1.exon1;Parent=MS.gene054152.t1
chr1.3 CDS 15113050 15113104 15113050 ID=cds.MS.gene054152.t1;Parent=MS.gene054152.t1
chr1.3 exon 15114153 15114553 15114153 ID=MS.gene054152.t1.exon2;Parent=MS.gene054152.t1
chr1.3 CDS 15114153 15114553 15114153 ID=cds.MS.gene054152.t1;Parent=MS.gene054152.t1
Gene Sequence

>MS.gene054152

ATGGAGCGTGGAAGTCCTTCATCTAACAACAACCGTTCGGCACACTCAAGCTTTGGTAAGCACTCTAAGCAAAAAAAAAATTACACATTTGTATCTCTTTTTAATGAGTTGAGATCCTCGTATTTTATTATTTTTAATTTTTTTTACTTTGTAAAACATTGAATCCGGTATCTAATTTCATAGTAGATCTTATTGTTTTAATATTTTTTCACTAGATTTTATTTTATTTTTCGTATTTTTTCCCCTTAATTGCTCTATTAGATTCTCAAGGCATTATTAGCCTCGGGCAAGAATGATCTAACTTGGGATATAATTGTGCATCACTTATGGGAAAATACTTAGTTTTTGAATGAAATTTTGATTGAAGGGGATGACAAAAAAAATATGCATCCCATATTTGACCTCTATCTCTTTTACTTAATTTTATCTATACCTTTAATATTTTTAATTTATTATTTTAACGTGTGTGAGTGTTATAAATTTTGGGGTGCTGAATTCGATTCACACTAAAAAATGTTTGAGATCTTGGAATTAATTTTGTTCGGAGCAGATCTGCGTATGCAATGGTGAGAAAAACAACTTTGCTAAGATTTCAATTTTTTAATATTATATATAAAACCATATAATATTAATTATAATAATAAATTAATTAATTAAGGTTACGCAAAAATGCAATTATCGTGTGAAAGAAATAAAGTGAGAGAAAAGGTGGGTGAAAAAATAAATAAGGCCCCTTTAAATACGTCAGTTTTGTTACAAATTTCATTATTGATCTATCAAATAGCGTGAAGCTAACCAATATACACACAGACAACCAAAATATGTTTGTATCTTAGTTGTAATAGCTTCATATGTCTCGTTTCTATATGTAATGATTCTACTAACGTCAAGTGATGTATGTGTAGTCACATTTTACAAGCTGGTTTGCCACCTTAGATTAAAAAGAAGAAAAGAAATTATACGTTACATTTTTTGTCTAAGTGAAATTTTTGTTAAAAGTTAAAAAGAAATTAATTTTCTCTTGTACCAAATCTATTAATTTTGAGGAGCTAAGATTTATTTTTTTTTTCTTTTTTCTTTTACCTCTATTAAAAACATCTTAGCTGAAAGAAACACCACCACCAACAACAACACTGGCATTGGAACTGAGGACCAACATTCAACTCCAGCAATGGCCGAGGAAGTGCGTATGCCCATCAGTCATGTGACAAGAGTCATGCAGAATGTTCTTCCACCAGACACAATAATCACTGATGATGCTAAGGAGATAATGCAATTGTTTGTGTCTAAATTTATGAACAAGGTCACAAGTGAGGCTTTCCAACAAGCCAATGTAGAACATCAAATGACTGTTTCTGCTGATGATTTACTATGGACGATGAATAGGTTGGGCTTTGAGGAATCTGTTCGGTCTCTTGGGAAATACCTCAAAAACTATCGCAAGAACATGGATGGTTCAGGTGCTAGTGGCTCAAACAATGCCAATGATGATGACCAACGTTGA
Protein sequence

>MS.gene054152.t1

MERGSPSSNNNRSAHSSFAERNTTTNNNTGIGTEDQHSTPAMAEEVRMPISHVTRVMQNVLPPDTIITDDAKEIMQLFVSKFMNKVTSEAFQQANVEHQMTVSADDLLWTMNRLGFEESVRSLGKYLKNYRKNMDGSGASGSNNANDDDQR