AlfalfaGEDB | MS.gene054304

M. sativa cultivar XinJiangDaYe / MS.gene054304

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054304.t1	GAU15485.1	64.8	91	28	1	18	108	78	164	1.70E-21	112.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054304.t1	Q9LT89	59.5	79	28	1	18	96	66	140	2.8e-17	89.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054304.t1	A0A1S2XYS3	63.0	92	28	2	18	108	83	169	6.0e-21	109.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene054304.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054304.t1	MTR_1g101810	63.043	92	28	2	18	108	103	189	1.39e-28	106
MS.gene054304.t1	MTR_1g101810	63.043	92	28	2	18	108	73	159	2.91e-28	105
MS.gene054304.t1	MTR_8g033070	56.818	88	31	3	18	104	89	170	4.07e-22	88.6
MS.gene054304.t1	MTR_7g028160	52.000	75	30	2	18	91	59	128	4.95e-16	71.6
MS.gene054304.t1	MTR_2g006150	47.423	97	41	3	9	100	126	217	4.05e-15	70.1
MS.gene054304.t1	MTR_2g006150	47.423	97	41	3	9	100	126	217	7.61e-15	69.3
MS.gene054304.t1	MTR_5g039600	49.351	77	35	1	18	94	76	148	2.82e-14	67.4
MS.gene054304.t1	MTR_1g063870	75.000	36	9	0	40	75	76	111	9.40e-13	62.0
MS.gene054304.t1	MTR_4g108370	60.345	58	19	1	18	75	106	159	1.26e-12	62.8
MS.gene054304.t1	MTR_1g114380	57.627	59	19	2	18	75	42	95	3.26e-12	60.8
MS.gene054304.t1	MTR_1g038650	70.000	40	12	0	36	75	55	94	3.35e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054304.t1	AT2G45680	58.065	93	32	3	18	108	84	171	9.90e-24	93.2
MS.gene054304.t1	AT5G51910	61.644	73	24	1	18	90	66	134	4.90e-22	87.8
MS.gene054304.t1	AT5G51910	61.644	73	24	1	18	90	66	134	4.90e-22	87.8
MS.gene054304.t1	AT1G69690	60.345	58	19	1	18	75	61	114	1.90e-14	67.8
MS.gene054304.t1	AT3G47620	59.322	59	18	2	18	75	126	179	4.43e-14	67.0
MS.gene054304.t1	AT1G35560	61.017	59	17	2	18	75	64	117	2.21e-13	64.7
MS.gene054304.t1	AT3G27010	57.895	57	20	1	18	74	87	139	3.42e-13	64.3
MS.gene054304.t1	AT3G27010	57.895	57	20	1	18	74	87	139	3.42e-13	64.3
MS.gene054304.t1	AT1G58100	45.783	83	41	1	18	100	69	147	1.66e-12	62.4
MS.gene054304.t1	AT5G23280	57.627	59	19	2	18	75	48	101	3.06e-12	61.2
MS.gene054304.t1	AT5G08330	57.627	59	19	2	18	75	40	93	3.26e-12	60.8
MS.gene054304.t1	AT1G72010	60.345	58	19	1	18	75	72	125	4.59e-12	61.2
MS.gene054304.t1	AT3G45150	49.091	55	24	1	18	72	26	76	8.30e-11	56.2

Find 36 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGCTTTGATGATTGC+AGG	0.311422	6.1:+24796396	None:intergenic
GGCTATGGCCGGAATTATTC+CGG	0.369975	6.1:+24796371	None:intergenic
AGAAACAATAGGGTGGTTAC+TGG	0.376057	6.1:-24796430	MS.gene054304:CDS
CAACTTTCCGCCCTCTGAAA+TGG	0.397867	6.1:+24796350	None:intergenic
CACCGGCACCGGAATAATTC+CGG	0.398178	6.1:-24796379	MS.gene054304:CDS
AAGTCTGATGGAGAAACAAT+AGG	0.400346	6.1:-24796441	MS.gene054304:CDS
TTCCAGTTGACGAGAGATCT+TGG	0.406107	6.1:-24796468	MS.gene054304:intron
GGTCTAGCTGCCGATGTTGT+TGG	0.412258	6.1:+24796321	None:intergenic
TTCAGAGGGCGGAAAGTTGA+AGG	0.466569	6.1:-24796346	MS.gene054304:CDS
TTGCTGACAGTGTGACTCTT+CGG	0.478708	6.1:+24796508	None:intergenic
ATCCACAAATTAACCAATTG+AGG	0.488844	6.1:+24796260	None:intergenic
TTCGTGTGACGGTCTTCTGA+TGG	0.489473	6.1:+24796546	None:intergenic
ATCATCAAAGCCACCGGCAC+CGG	0.497106	6.1:-24796390	MS.gene054304:CDS
AGCTAGACCAGACGGCCAAA+AGG	0.501805	6.1:-24796307	MS.gene054304:CDS
CCCTCTGAAATGGCTATGGC+CGG	0.502325	6.1:+24796360	None:intergenic
AAAAGCAAGAGAGCCTCAAT+TGG	0.505951	6.1:-24796273	MS.gene054304:CDS
AAAATAGACGTTGAACCCAC+TGG	0.511072	6.1:-24796601	MS.gene054304:CDS
GGCCGGAATTATTCCGGTGC+CGG	0.511782	6.1:+24796377	None:intergenic
AGTCTGATGGAGAAACAATA+GGG	0.517180	6.1:-24796440	MS.gene054304:CDS
TCCGCCCTCTGAAATGGCTA+TGG	0.528278	6.1:+24796356	None:intergenic
CGGAATTATTCCGGTGCCGG+TGG	0.529649	6.1:+24796380	None:intergenic
GTCCCTCCACCTTCGTGTGA+CGG	0.543321	6.1:+24796535	None:intergenic
GATCTTGGTCACAAGTCTGA+TGG	0.556205	6.1:-24796453	MS.gene054304:CDS
AGACCGTCACACGAAGGTGG+AGG	0.561466	6.1:-24796538	MS.gene054304:CDS
CAGACGGCCAAAAGGCCCCA+AGG	0.564342	6.1:-24796299	MS.gene054304:CDS
GACCGTCACACGAAGGTGGA+GGG	0.603506	6.1:-24796537	MS.gene054304:CDS
TCCGGCCATAGCCATTTCAG+AGG	0.619818	6.1:-24796361	MS.gene054304:CDS
GCCATAGCCATTTCAGAGGG+CGG	0.621097	6.1:-24796357	MS.gene054304:CDS
AGAAGACCGTCACACGAAGG+TGG	0.622175	6.1:-24796541	MS.gene054304:CDS
CCTGCAATCATCAAAGCCAC+CGG	0.628378	6.1:-24796396	MS.gene054304:CDS
CCGGCCATAGCCATTTCAGA+GGG	0.636688	6.1:-24796360	MS.gene054304:CDS
ACATCGGCAGCTAGACCAGA+CGG	0.655173	6.1:-24796315	MS.gene054304:CDS
GTTGAAGGTTCCAACAACAT+CGG	0.665250	6.1:-24796331	MS.gene054304:CDS
GGAACCACAAAAGACAGTGT+CGG	0.676346	6.1:-24796580	MS.gene054304:CDS
ATCAGAAGACCGTCACACGA+AGG	0.685962	6.1:-24796544	MS.gene054304:CDS
CTGATGGAGAAACAATAGGG+TGG	0.705438	6.1:-24796437	MS.gene054304:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCTGATGGAGAAACAAT+AGG	-	chr6.1:24796438-24796457	MS.gene054304:CDS	35.0%
	AGTCTGATGGAGAAACAATA+GGG	-	chr6.1:24796439-24796458	MS.gene054304:CDS	35.0%
!	GTCTTCTGATGGTTTCTTTT+CGG	+	chr6.1:24796325-24796344	None:intergenic	35.0%
	AAAAGCAAGAGAGCCTCAAT+TGG	-	chr6.1:24796606-24796625	MS.gene054304:CDS	40.0%
	AAAATAGACGTTGAACCCAC+TGG	-	chr6.1:24796278-24796297	MS.gene054304:CDS	40.0%
	AGAAACAATAGGGTGGTTAC+TGG	-	chr6.1:24796449-24796468	MS.gene054304:CDS	40.0%
	GTTGAAGGTTCCAACAACAT+CGG	-	chr6.1:24796548-24796567	MS.gene054304:CDS	40.0%
!!	CTCTCTTGCTTTTCTTCCTT+GGG	+	chr6.1:24796599-24796618	None:intergenic	40.0%
!!	TCTCTTGCTTTTCTTCCTTG+GGG	+	chr6.1:24796598-24796617	None:intergenic	40.0%
	CTGATGGAGAAACAATAGGG+TGG	-	chr6.1:24796442-24796461	MS.gene054304:CDS	45.0%
	GATCTTGGTCACAAGTCTGA+TGG	-	chr6.1:24796426-24796445	MS.gene054304:CDS	45.0%
	GGAACCACAAAAGACAGTGT+CGG	-	chr6.1:24796299-24796318	MS.gene054304:CDS	45.0%
	TGTCAGCAACATGTGCATCT+AGG	-	chr6.1:24796385-24796404	MS.gene054304:CDS	45.0%
	TTCCAGTTGACGAGAGATCT+TGG	-	chr6.1:24796411-24796430	MS.gene054304:CDS	45.0%
	TTGCTGACAGTGTGACTCTT+CGG	+	chr6.1:24796374-24796393	None:intergenic	45.0%
!	ACTGTCTTTTGTGGTTCCAG+TGG	+	chr6.1:24796297-24796316	None:intergenic	45.0%
!	CTGTCTTTTGTGGTTCCAGT+GGG	+	chr6.1:24796296-24796315	None:intergenic	45.0%
!	TTTTCTTCCTTGGGGCCTTT+TGG	+	chr6.1:24796590-24796609	None:intergenic	45.0%
!!	GCTCTCTTGCTTTTCTTCCT+TGG	+	chr6.1:24796600-24796619	None:intergenic	45.0%
	ATCAGAAGACCGTCACACGA+AGG	-	chr6.1:24796335-24796354	MS.gene054304:CDS	50.0%
	CCTGCAATCATCAAAGCCAC+CGG	-	chr6.1:24796483-24796502	MS.gene054304:intron	50.0%
	GACCAAGATCTCTCGTCAAC+TGG	+	chr6.1:24796416-24796435	None:intergenic	50.0%
	GGCTATGGCCGGAATTATTC+CGG	+	chr6.1:24796511-24796530	None:intergenic	50.0%
	TTCAGAGGGCGGAAAGTTGA+AGG	-	chr6.1:24796533-24796552	MS.gene054304:CDS	50.0%
	TTCGTGTGACGGTCTTCTGA+TGG	+	chr6.1:24796336-24796355	None:intergenic	50.0%
!	CAACTTTCCGCCCTCTGAAA+TGG	+	chr6.1:24796532-24796551	None:intergenic	50.0%
	ACATCGGCAGCTAGACCAGA+CGG	-	chr6.1:24796564-24796583	MS.gene054304:CDS	55.0%
	AGAAGACCGTCACACGAAGG+TGG	-	chr6.1:24796338-24796357	MS.gene054304:CDS	55.0%
	AGCTAGACCAGACGGCCAAA+AGG	-	chr6.1:24796572-24796591	MS.gene054304:CDS	55.0%
	ATCATCAAAGCCACCGGCAC+CGG	-	chr6.1:24796489-24796508	MS.gene054304:intron	55.0%
	CCGGCCATAGCCATTTCAGA+GGG	-	chr6.1:24796519-24796538	MS.gene054304:CDS	55.0%
	GCCATAGCCATTTCAGAGGG+CGG	-	chr6.1:24796522-24796541	MS.gene054304:CDS	55.0%
	GGTCTAGCTGCCGATGTTGT+TGG	+	chr6.1:24796561-24796580	None:intergenic	55.0%
	TCCGGCCATAGCCATTTCAG+AGG	-	chr6.1:24796518-24796537	MS.gene054304:CDS	55.0%
!	CACCGGCACCGGAATAATTC+CGG	-	chr6.1:24796500-24796519	MS.gene054304:intron	55.0%
!	TCGGCCGACACTGTCTTTTG+TGG	+	chr6.1:24796306-24796325	None:intergenic	55.0%
!!	CCCTCTGAAATGGCTATGGC+CGG	+	chr6.1:24796522-24796541	None:intergenic	55.0%
!!	CCGGTGGCTTTGATGATTGC+AGG	+	chr6.1:24796486-24796505	None:intergenic	55.0%
!!	TCCGCCCTCTGAAATGGCTA+TGG	+	chr6.1:24796526-24796545	None:intergenic	55.0%
	AGACCGTCACACGAAGGTGG+AGG	-	chr6.1:24796341-24796360	MS.gene054304:CDS	60.0%
	GACCGTCACACGAAGGTGGA+GGG	-	chr6.1:24796342-24796361	MS.gene054304:CDS	60.0%
	GGCCGGAATTATTCCGGTGC+CGG	+	chr6.1:24796505-24796524	None:intergenic	60.0%
	GTCCCTCCACCTTCGTGTGA+CGG	+	chr6.1:24796347-24796366	None:intergenic	60.0%
!	CGGAATTATTCCGGTGCCGG+TGG	+	chr6.1:24796502-24796521	None:intergenic	60.0%
	CAGACGGCCAAAAGGCCCCA+AGG	-	chr6.1:24796580-24796599	MS.gene054304:CDS	65.0%
!	CTTGGGGCCTTTTGGCCGTC+TGG	+	chr6.1:24796582-24796601	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	24796269	24796632	24796269	ID=MS.gene054304
chr6.1	mRNA	24796269	24796632	24796269	ID=MS.gene054304.t1;Parent=MS.gene054304
chr6.1	exon	24796516	24796632	24796516	ID=MS.gene054304.t1.exon1;Parent=MS.gene054304.t1
chr6.1	CDS	24796516	24796632	24796516	ID=cds.MS.gene054304.t1;Parent=MS.gene054304.t1
chr6.1	exon	24796269	24796484	24796269	ID=MS.gene054304.t1.exon2;Parent=MS.gene054304.t1
chr6.1	CDS	24796269	24796484	24796269	ID=cds.MS.gene054304.t1;Parent=MS.gene054304.t1

Gene Sequence	>MS.gene054304 ATGCATGGAAAAATAGACGTTGAACCCACTGGAACCACAAAAGACAGTGTCGGCCGAAAAGAAACCATCAGAAGACCGTCACACGAAGGTGGAGGGACGTGTCCGAAGAGTCACACTGTCAGCAACATGTGCATCTAGGATCTTCCAGTTGACGAGAGATCTTGGTCACAAGTCTGATGGAGAAACAATAGGGTGGTTACTGGAACAAGCTGAACCTGCAATCATCAAAGCCACCGGCACCGGAATAATTCCGGCCATAGCCATTTCAGAGGGCGGAAAGTTGAAGGTTCCAACAACATCGGCAGCTAGACCAGACGGCCAAAAGGCCCCAAGGAAGAAAAGCAAGAGAGCCTCAATTGGTTAA
Protein sequence	>MS.gene054304.t1 MHGKIDVEPTGTTKDSVGRKETIRRPSHEGGGTCPKSHTLTRDLGHKSDGETIGWLLEQAEPAIIKATGTGIIPAIAISEGGKLKVPTTSAARPDGQKAPRKKSKRASIG