AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054311

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054311.t1 MTR_6g043060 70.642 327 66 2 1 297 1 327 3.45e-165 462
MS.gene054311.t1 MTR_8g083100 75.540 278 24 1 23 256 23 300 2.04e-146 420
MS.gene054311.t1 MTR_8g083100 79.545 44 9 0 256 299 414 457 1.97e-15 76.6
MS.gene054311.t1 MTR_2g100680 58.140 301 79 3 1 256 12 310 1.14e-113 336
MS.gene054311.t1 MTR_6g044340 64.062 256 68 2 68 299 8 263 1.36e-109 318
MS.gene054311.t1 MTR_2g100670 56.184 283 92 6 1 254 14 293 1.32e-96 287
MS.gene054311.t1 MTR_2g100670 56.184 283 92 6 1 254 122 401 8.51e-95 286
MS.gene054311.t1 MTR_5g029680 50.237 211 91 4 22 218 32 242 5.26e-64 209
MS.gene054311.t1 MTR_8g087540 49.275 207 95 3 15 216 5 206 8.82e-60 196
MS.gene054311.t1 MTR_6g044430 48.387 248 40 5 100 295 14 225 1.82e-56 182
MS.gene054311.t1 MTR_4g077970 44.498 209 104 3 20 216 10 218 8.73e-55 185
MS.gene054311.t1 MTR_6g044440 54.749 179 28 2 174 299 1 179 4.22e-53 172
MS.gene054311.t1 MTR_1g102860 44.660 206 102 5 19 215 37 239 9.36e-51 170
MS.gene054311.t1 MTR_7g095680 45.411 207 103 4 19 215 46 252 2.04e-50 170
MS.gene054311.t1 MTR_3g104550 35.000 300 173 7 19 297 43 341 8.54e-50 169
MS.gene054311.t1 MTR_1g061170 41.000 200 115 2 20 216 47 246 3.00e-47 164
MS.gene054311.t1 MTR_1g071360 40.553 217 117 3 22 233 32 241 2.58e-46 159
MS.gene054311.t1 MTR_1g071360 40.553 217 117 3 22 233 29 238 2.96e-46 160
MS.gene054311.t1 MTR_8g105780 48.205 195 88 6 20 201 20 214 2.28e-45 157
MS.gene054311.t1 MTR_3g101870 66.667 102 34 0 12 113 14 115 4.07e-44 154
MS.gene054311.t1 MTR_6g086805 40.187 214 101 2 20 219 9 209 6.92e-42 147
MS.gene054311.t1 MTR_2g017890 59.259 108 44 0 12 119 5 112 1.60e-41 147
MS.gene054311.t1 MTR_5g017470 62.745 102 38 0 21 122 8 109 1.67e-41 145
MS.gene054311.t1 MTR_5g089170 64.000 100 36 0 14 113 17 116 7.86e-41 142
MS.gene054311.t1 MTR_6g044350 54.795 146 32 3 174 285 1 146 3.84e-40 137
MS.gene054311.t1 MTR_5g010680 57.407 108 46 0 6 113 8 115 1.09e-39 142
MS.gene054311.t1 MTR_7g091370 51.327 113 55 0 1 113 1 113 3.41e-37 132
MS.gene054311.t1 MTR_0020s0020 61.290 93 36 0 21 113 32 124 6.62e-37 134
MS.gene054311.t1 MTR_1g100777 58.947 95 39 0 21 115 17 111 3.46e-36 132
MS.gene054311.t1 MTR_4g088900 56.701 97 38 1 21 113 32 128 7.81e-33 120
MS.gene054311.t1 MTR_5g047190 45.361 97 46 2 30 119 1 97 3.40e-21 86.7
MS.gene054311.t1 MTR_6g044510 61.972 71 19 1 237 299 18 88 4.21e-21 85.9
MS.gene054311.t1 MTR_4g045757 60.870 69 26 1 12 79 3 71 7.99e-21 84.7
MS.gene054311.t1 MTR_1g061170 30.818 159 99 4 61 216 1 151 1.64e-13 70.5
MS.gene054311.t1 MTR_6g044500 47.561 82 17 3 205 261 1 81 1.60e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054311.t1 AT4G13980 55.983 234 101 2 1 232 1 234 2.37e-85 264
MS.gene054311.t1 AT4G17750 47.685 216 92 4 22 216 52 267 9.21e-59 196
MS.gene054311.t1 AT1G32330 45.957 235 108 5 1 216 16 250 2.19e-58 194
MS.gene054311.t1 AT1G32330 45.957 235 108 5 1 216 16 250 2.41e-58 194
MS.gene054311.t1 AT5G16820 47.867 211 95 5 20 216 25 234 6.59e-58 193
MS.gene054311.t1 AT5G16820 47.867 211 95 5 20 216 25 234 6.59e-58 193
MS.gene054311.t1 AT3G02990 42.918 233 120 4 8 228 9 240 1.16e-57 192
MS.gene054311.t1 AT5G45710 38.462 312 153 8 14 295 5 307 3.13e-55 182
MS.gene054311.t1 AT5G45710 38.462 312 153 8 14 295 5 307 3.13e-55 182
MS.gene054311.t1 AT4G18880 48.815 211 98 3 16 220 9 215 6.61e-53 178
MS.gene054311.t1 AT2G26150 42.342 222 120 4 19 232 41 262 1.49e-50 170
MS.gene054311.t1 AT2G26150 42.342 222 120 4 19 232 41 262 1.49e-50 170
MS.gene054311.t1 AT3G51910 42.180 211 105 5 22 226 29 228 2.42e-47 160
MS.gene054311.t1 AT3G22830 39.815 216 117 3 19 221 58 273 6.76e-47 162
MS.gene054311.t1 AT5G03720 42.079 202 109 3 20 213 53 254 5.04e-46 160
MS.gene054311.t1 AT5G03720 42.079 202 109 3 20 213 53 254 5.04e-46 160
MS.gene054311.t1 AT3G24520 45.665 173 74 3 20 191 15 168 2.21e-44 154
MS.gene054311.t1 AT1G46264 61.607 112 33 1 21 122 33 144 2.64e-43 151
MS.gene054311.t1 AT1G67970 44.271 192 94 5 20 201 17 205 2.75e-43 152
MS.gene054311.t1 AT5G43840 41.711 187 97 3 21 205 18 194 1.10e-41 145
MS.gene054311.t1 AT5G54070 42.623 183 99 2 20 201 69 246 1.73e-40 144
MS.gene054311.t1 AT3G63350 31.655 278 163 7 19 295 25 276 4.10e-39 139
MS.gene054311.t1 AT4G36990 46.667 165 55 3 21 153 13 176 6.96e-38 135
MS.gene054311.t1 AT4G11660 62.366 93 35 0 21 113 58 150 3.97e-37 136
MS.gene054311.t1 AT5G62020 49.580 119 59 1 1 118 1 119 1.08e-36 133
MS.gene054311.t1 AT2G41690 51.304 115 56 0 22 136 40 154 3.42e-36 130
MS.gene054311.t1 AT4G18870 46.591 88 47 0 22 109 146 233 5.11e-22 94.0
MS.gene054311.t1 AT4G18870 45.833 96 51 1 14 109 5 99 1.17e-21 92.8
MS.gene054311.t1 AT1G77570 42.391 92 49 3 19 106 22 113 3.07e-17 77.4
MS.gene054311.t1 AT2G26150 31.500 200 122 6 43 232 13 207 9.15e-16 76.3
MS.gene054311.t1 AT2G26150 31.500 200 122 6 43 232 13 207 9.15e-16 76.3
MS.gene054311.t1 AT5G45710 29.545 220 116 9 106 295 1 211 7.11e-14 70.5
MS.gene054311.t1 AT5G45710 29.545 220 116 9 106 295 1 211 7.11e-14 70.5
MS.gene054311.t1 AT4G19630 34.021 97 61 1 19 112 8 104 1.12e-12 64.3

Find 70 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCAATGCTGCCCTTTC+TGG -0.010683 6.1:+24591430 None:intergenic
TTTCTTTGAGAAGGCTCTTC+AGG 0.261460 6.1:-24591263 MS.gene054311:CDS
GAAAATCAGATCCTGATCTT+TGG 0.266552 6.1:-24591561 MS.gene054311:CDS
GAAATTGCTGTGTTTGAAAT+AGG 0.280393 6.1:+24592636 None:intergenic
ATAATTATAGCTTAAGAGTT+TGG 0.284728 6.1:+24591366 None:intergenic
CCACAGATGAGATCGTTTCT+TGG 0.319260 6.1:-24592723 MS.gene054311:CDS
CAAAATCGAATCCATGGATT+TGG 0.330894 6.1:-24591212 MS.gene054311:CDS
AAGGAGTGAATGATGTGTTT+TGG 0.360172 6.1:-24590994 MS.gene054311:CDS
ATAACAAAAGCTTCATCGTT+TGG 0.362747 6.1:-24592693 MS.gene054311:CDS
AGAAGAACGCGAGAGAATTC+AGG 0.373908 6.1:+24592664 None:intergenic
AAAATCAGATCCTGATCTTT+GGG 0.374128 6.1:-24591560 MS.gene054311:CDS
ACGTTACCTGAAGCCTCCTT+TGG 0.398346 6.1:+24591040 None:intergenic
TAAGAAAAGGCGATTGTCTC+AGG 0.414889 6.1:-24591179 MS.gene054311:CDS
ACCAAGCTACCTTCGGCAAC+AGG 0.417714 6.1:+24591142 None:intergenic
AGCTTAAGAGTTTGGATTGA+AGG 0.419735 6.1:+24591374 None:intergenic
TGCTGTGTTTGAAATAGGTT+GGG 0.448167 6.1:+24592641 None:intergenic
TTGCTGTGTTTGAAATAGGT+TGG 0.457272 6.1:+24592640 None:intergenic
TGGAAACTTATAATAAGAAA+AGG 0.461564 6.1:-24591192 MS.gene054311:CDS
GCCGAGCAGCTGCTTCGACT+TGG 0.465666 6.1:+24591017 None:intergenic
CCACAGTCATAGTCATCCTC+CGG 0.469987 6.1:-24591467 MS.gene054311:CDS
TTCCCGCAAAATCGAATCCA+TGG 0.478636 6.1:-24591218 MS.gene054311:CDS
GCTTAAGAGTTTGGATTGAA+GGG 0.480417 6.1:+24591375 None:intergenic
ATTATAAGTTTCCAAATCCA+TGG 0.481800 6.1:+24591201 None:intergenic
GCTGATCCAAAGGAGGCTTC+AGG 0.482903 6.1:-24591046 MS.gene054311:CDS
TTTGCAGAATTTCTTTGAGA+AGG 0.483479 6.1:-24591272 MS.gene054311:CDS
GAAGCCTCCTTTGGATCAGC+GGG 0.484852 6.1:+24591049 None:intergenic
AGATCCAGAAAGGGCAGCAT+TGG 0.494908 6.1:-24591434 MS.gene054311:CDS
TTTGCTTCCGTCGGCAGCAG+TGG 0.495690 6.1:+24592798 None:intergenic
TGAGACAGAAAAGCTTCAGC+TGG 0.500611 6.1:-24591341 MS.gene054311:CDS
TTGGCAAACTCCCAAAGATC+AGG 0.503856 6.1:+24591550 None:intergenic
ATTGAGCTGACGGATGAAGC+TGG 0.506271 6.1:+24592612 None:intergenic
GCCTGTTGCCGAAGGTAGCT+TGG 0.510527 6.1:-24591143 MS.gene054311:CDS
TTCTCTCAGAATCTTTCTGA+AGG 0.511282 6.1:-24591103 MS.gene054311:CDS
AACTGACCCCGCTGATCCAA+AGG 0.526560 6.1:-24591056 MS.gene054311:CDS
CTTTCTGAAGGAGAACTGAC+AGG 0.534056 6.1:-24591091 MS.gene054311:CDS
CGACGGAAGCAAAGAAGGTA+TGG 0.536953 6.1:-24592788 MS.gene054311:CDS
GCTGCCGACGGAAGCAAAGA+AGG 0.537828 6.1:-24592793 MS.gene054311:CDS
GTCGAAGCAGCTGCTCGGCA+AGG 0.540320 6.1:-24591013 MS.gene054311:CDS
AACAGCATGGACTTTCAGTA+AGG 0.541453 6.1:+24590963 None:intergenic
AAGGTCAACCAAGCTACCTT+CGG 0.541708 6.1:+24591135 None:intergenic
TCTGAGAGAAATTGCTAGGA+AGG 0.542451 6.1:+24591116 None:intergenic
TCATACGTCTTCTGAAGAAA+CGG 0.543831 6.1:+24592763 None:intergenic
TCCAAGTCGAAGCAGCTGCT+CGG 0.548536 6.1:-24591018 MS.gene054311:CDS
TCAGCGGCGATTGGAAGGCA+TGG 0.549084 6.1:-24591311 MS.gene054311:CDS
CCGGAGGATGACTATGACTG+TGG 0.551542 6.1:+24591467 None:intergenic
CACAGTCATAGTCATCCTCC+GGG 0.559092 6.1:-24591466 MS.gene054311:CDS
TTCTGCTGTAGATCCAGAAA+GGG 0.565003 6.1:-24591443 MS.gene054311:CDS
AGATTCTGAGAGAAATTGCT+AGG 0.568077 6.1:+24591112 None:intergenic
GTTCTGCTGTAGATCCAGAA+AGG 0.569845 6.1:-24591444 MS.gene054311:CDS
GACTGTGGATAGGTTTCTTG+CGG 0.574041 6.1:+24591482 None:intergenic
AGAAACGATCTCATCTGTGG+TGG 0.582151 6.1:+24592726 None:intergenic
CATACCTTCTTTGCTTCCGT+CGG 0.584464 6.1:+24592789 None:intergenic
TGAAGCCTCCTTTGGATCAG+CGG 0.591974 6.1:+24591048 None:intergenic
GATTGGAAGGCATGGAGAAG+AGG 0.593047 6.1:-24591303 MS.gene054311:CDS
CACATGCAGCCTGTTGCCGA+AGG 0.596050 6.1:-24591151 MS.gene054311:CDS
TCTTCATTGTCTGAACAGCA+TGG 0.597736 6.1:+24590950 None:intergenic
TTACATAGGTATTGAGCTGA+CGG 0.598207 6.1:+24592602 None:intergenic
TCATCAGCCACTGCTGCCGA+CGG 0.606067 6.1:-24592805 MS.gene054311:CDS
TCTGGATCTACAGCAGAACC+CGG 0.614712 6.1:+24591448 None:intergenic
GGATGACTATGACTGTGGAT+AGG 0.617132 6.1:+24591472 None:intergenic
GAAACGATCTCATCTGTGGT+GGG 0.626600 6.1:+24592727 None:intergenic
AGAACGCGAGAGAATTCAGG+AGG 0.628084 6.1:+24592667 None:intergenic
CCAAGAAACGATCTCATCTG+TGG 0.633047 6.1:+24592723 None:intergenic
ATGCTGTTCAGACAATGAAG+AGG 0.637659 6.1:-24590948 MS.gene054311:CDS
TGACCCCGCTGATCCAAAGG+AGG 0.662370 6.1:-24591053 MS.gene054311:CDS
GAACGCGAGAGAATTCAGGA+GGG 0.662504 6.1:+24592668 None:intergenic
TCTTCAGAAGACGTATGACA+TGG 0.665030 6.1:-24592758 MS.gene054311:CDS
GCAGTGGCTGATGATGATTG+TGG 0.706595 6.1:+24592814 None:intergenic
AAGCCTCCTTTGGATCAGCG+GGG 0.731334 6.1:+24591050 None:intergenic
GGATCTACAGCAGAACCCGG+AGG 0.744225 6.1:+24591451 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTATAAATTTTTCAT+TGG + chr6.1:24592232-24592251 None:intergenic 10.0%
!! TTATCATATTTATATAGTTC+AGG + chr6.1:24591843-24591862 None:intergenic 15.0%
!!! ATGTATGTTTTAATTTGTTA+CGG - chr6.1:24592144-24592163 MS.gene054311:intron 15.0%
!! AGGATTAAACAATAAAAATC+AGG + chr6.1:24591872-24591891 None:intergenic 20.0%
!! ATAATTATAGCTTAAGAGTT+TGG + chr6.1:24592397-24592416 None:intergenic 20.0%
!! ATATTCTTGTAGAGATTTAA+TGG - chr6.1:24592069-24592088 MS.gene054311:intron 20.0%
!! ATTATTTCATTGAGTTTAGT+TGG - chr6.1:24591419-24591438 MS.gene054311:CDS 20.0%
!! TGATTAGTAAGATAAGAATT+AGG - chr6.1:24591379-24591398 MS.gene054311:CDS 20.0%
!! TGGAAACTTATAATAAGAAA+AGG - chr6.1:24592568-24592587 MS.gene054311:intron 20.0%
!!! TTTGTTGTATTTGTATGATT+TGG - chr6.1:24591273-24591292 MS.gene054311:CDS 20.0%
! AAATAGAAATCATGCACTTA+TGG - chr6.1:24591984-24592003 MS.gene054311:intron 25.0%
! AATGGTAAATCGTTCAAAAT+AGG - chr6.1:24592087-24592106 MS.gene054311:intron 25.0%
! ATACTGAATTATGTCATTGT+CGG + chr6.1:24591573-24591592 None:intergenic 25.0%
! ATATTCAGAGACTAAATTGA+TGG + chr6.1:24591799-24591818 None:intergenic 25.0%
! ATTAGGTACAACTTTAGATT+TGG - chr6.1:24591396-24591415 MS.gene054311:CDS 25.0%
! ATTATAAGTTTCCAAATCCA+TGG + chr6.1:24592562-24592581 None:intergenic 25.0%
! CTTCATTAAACAAAAACTTC+AGG + chr6.1:24592048-24592067 None:intergenic 25.0%
! TGATATCTAAGTCTTAGATA+GGG - chr6.1:24591956-24591975 MS.gene054311:intron 25.0%
! TTCAGTATAATCATATGTGT+CGG - chr6.1:24591585-24591604 MS.gene054311:intron 25.0%
! TTGATATCTAAGTCTTAGAT+AGG - chr6.1:24591955-24591974 MS.gene054311:intron 25.0%
!!! ATTTATGTGTGCTTTTTATC+AGG - chr6.1:24592170-24592189 MS.gene054311:intron 25.0%
!!! TTTATGTGTGCTTTTTATCA+GGG - chr6.1:24592171-24592190 MS.gene054311:intron 25.0%
AAAAAAATTTCTGCAGCATC+TGG - chr6.1:24591736-24591755 MS.gene054311:intron 30.0%
AAAATCAGATCCTGATCTTT+GGG - chr6.1:24592200-24592219 MS.gene054311:intron 30.0%
AGATCAAAAACATCTTCTGA+CGG - chr6.1:24592248-24592267 MS.gene054311:intron 30.0%
ATAACAAAAGCTTCATCGTT+TGG - chr6.1:24591067-24591086 MS.gene054311:CDS 30.0%
ATATAGTTCAGGAACTAAAC+CGG + chr6.1:24591832-24591851 None:intergenic 30.0%
ATCGTTCAAAATAGGTCTTA+TGG - chr6.1:24592095-24592114 MS.gene054311:intron 30.0%
CTTCATCGAATTTCAATTAG+TGG - chr6.1:24591307-24591326 MS.gene054311:CDS 30.0%
TCTGAATATCACACTATTTC+CGG - chr6.1:24591810-24591829 MS.gene054311:intron 30.0%
! GAAATTGCTGTGTTTGAAAT+AGG + chr6.1:24591127-24591146 None:intergenic 30.0%
! TTTGCAGAATTTCTTTGAGA+AGG - chr6.1:24592488-24592507 MS.gene054311:intron 30.0%
!! CGAGATATTATAGTCTTGTA+TGG - chr6.1:24591482-24591501 MS.gene054311:CDS 30.0%
AAAACTTCAGGAAGCAAATC+TGG + chr6.1:24592036-24592055 None:intergenic 35.0%
AGCTTAAGAGTTTGGATTGA+AGG + chr6.1:24592389-24592408 None:intergenic 35.0%
CAAAATCGAATCCATGGATT+TGG - chr6.1:24592548-24592567 MS.gene054311:intron 35.0%
GAAAATCAGATCCTGATCTT+TGG - chr6.1:24592199-24592218 MS.gene054311:intron 35.0%
GACATGAGAATGTTGAAAGA+TGG - chr6.1:24591458-24591477 MS.gene054311:CDS 35.0%
GCTTAAGAGTTTGGATTGAA+GGG + chr6.1:24592388-24592407 None:intergenic 35.0%
TTCTCTCAGAATCTTTCTGA+AGG - chr6.1:24592657-24592676 MS.gene054311:CDS 35.0%
! AAGGAGTGAATGATGTGTTT+TGG - chr6.1:24592766-24592785 MS.gene054311:CDS 35.0%
! AATCCATGGATTCGATTTTG+CGG + chr6.1:24592548-24592567 None:intergenic 35.0%
! AGATTCTGAGAGAAATTGCT+AGG + chr6.1:24592651-24592670 None:intergenic 35.0%
! AGGAGTGAATGATGTGTTTT+GGG - chr6.1:24592767-24592786 MS.gene054311:CDS 35.0%
! TAGCGTGAATTGACTTACAT+AGG + chr6.1:24591175-24591194 None:intergenic 35.0%
! TCATACGTCTTCTGAAGAAA+CGG + chr6.1:24591000-24591019 None:intergenic 35.0%
! TGCTGTGTTTGAAATAGGTT+GGG + chr6.1:24591122-24591141 None:intergenic 35.0%
! TGTGAATTGGTGGGTAATTT+AGG - chr6.1:24591685-24591704 MS.gene054311:intron 35.0%
! TTGCTGTGTTTGAAATAGGT+TGG + chr6.1:24591123-24591142 None:intergenic 35.0%
!! TTACATAGGTATTGAGCTGA+CGG + chr6.1:24591161-24591180 None:intergenic 35.0%
AAATTCAGACCTACGAAGCA+AGG + chr6.1:24591653-24591672 None:intergenic 40.0%
AACAGCATGGACTTTCAGTA+AGG + chr6.1:24592800-24592819 None:intergenic 40.0%
ATGCTGTTCAGACAATGAAG+AGG - chr6.1:24592812-24592831 MS.gene054311:CDS 40.0%
TAAGAAAAGGCGATTGTCTC+AGG - chr6.1:24592581-24592600 MS.gene054311:intron 40.0%
TCTGAGAGAAATTGCTAGGA+AGG + chr6.1:24592647-24592666 None:intergenic 40.0%
TCTTCAGAAGACGTATGACA+TGG - chr6.1:24591002-24591021 MS.gene054311:CDS 40.0%
TCTTCATTGTCTGAACAGCA+TGG + chr6.1:24592813-24592832 None:intergenic 40.0%
TTCGTTGTTTGTGATGTGTC+TGG + chr6.1:24591541-24591560 None:intergenic 40.0%
TTCTGCTGTAGATCCAGAAA+GGG - chr6.1:24592317-24592336 MS.gene054311:intron 40.0%
! ATCCATGGATTCGATTTTGC+GGG + chr6.1:24592547-24592566 None:intergenic 40.0%
! GCTTCGTAGGTCTGAATTTT+CGG - chr6.1:24591654-24591673 MS.gene054311:intron 40.0%
! TTCAGCTGGAAGATTTTCAG+CGG - chr6.1:24592433-24592452 MS.gene054311:intron 40.0%
! TTTCTTTGAGAAGGCTCTTC+AGG - chr6.1:24592497-24592516 MS.gene054311:intron 40.0%
!! CATCTGGATCTTGGTAATGA+TGG - chr6.1:24591752-24591771 MS.gene054311:intron 40.0%
!! TAGGTCTGAATTTTCGGTGT+GGG - chr6.1:24591660-24591679 MS.gene054311:intron 40.0%
AAGGTCAACCAAGCTACCTT+CGG + chr6.1:24592628-24592647 None:intergenic 45.0%
ACTTCAGGAAGCAAATCTGG+CGG + chr6.1:24592033-24592052 None:intergenic 45.0%
AGAAACGATCTCATCTGTGG+TGG + chr6.1:24591037-24591056 None:intergenic 45.0%
AGAAGAACGCGAGAGAATTC+AGG + chr6.1:24591099-24591118 None:intergenic 45.0%
CATACCTTCTTTGCTTCCGT+CGG + chr6.1:24590974-24590993 None:intergenic 45.0%
CCAAGAAACGATCTCATCTG+TGG + chr6.1:24591040-24591059 None:intergenic 45.0%
CCACAGATGAGATCGTTTCT+TGG - chr6.1:24591037-24591056 MS.gene054311:CDS 45.0%
CGTAGACATCTCAAGCATCT+AGG - chr6.1:24591907-24591926 MS.gene054311:intron 45.0%
GAAACGATCTCATCTGTGGT+GGG + chr6.1:24591036-24591055 None:intergenic 45.0%
GACTGTGGATAGGTTTCTTG+CGG + chr6.1:24592281-24592300 None:intergenic 45.0%
GGATGACTATGACTGTGGAT+AGG + chr6.1:24592291-24592310 None:intergenic 45.0%
GTTCTGCTGTAGATCCAGAA+AGG - chr6.1:24592316-24592335 MS.gene054311:intron 45.0%
TGAGACAGAAAAGCTTCAGC+TGG - chr6.1:24592419-24592438 MS.gene054311:intron 45.0%
TTCCCGCAAAATCGAATCCA+TGG - chr6.1:24592542-24592561 MS.gene054311:intron 45.0%
TTGGCAAACTCCCAAAGATC+AGG + chr6.1:24592213-24592232 None:intergenic 45.0%
TTTCTGCAGCATCTGGATCT+TGG - chr6.1:24591743-24591762 MS.gene054311:intron 45.0%
! CTTTCTGAAGGAGAACTGAC+AGG - chr6.1:24592669-24592688 MS.gene054311:CDS 45.0%
! GTAGGTCTGAATTTTCGGTG+TGG - chr6.1:24591659-24591678 MS.gene054311:intron 45.0%
ACGTTACCTGAAGCCTCCTT+TGG + chr6.1:24592723-24592742 None:intergenic 50.0%
AGAACGCGAGAGAATTCAGG+AGG + chr6.1:24591096-24591115 None:intergenic 50.0%
AGATCCAGAAAGGGCAGCAT+TGG - chr6.1:24592326-24592345 MS.gene054311:intron 50.0%
CACAGTCATAGTCATCCTCC+GGG - chr6.1:24592294-24592313 MS.gene054311:intron 50.0%
CCACAGTCATAGTCATCCTC+CGG - chr6.1:24592293-24592312 MS.gene054311:intron 50.0%
CGACGGAAGCAAAGAAGGTA+TGG - chr6.1:24590972-24590991 MS.gene054311:CDS 50.0%
CTACGATGCAGAAGAGAAGC+AGG + chr6.1:24591892-24591911 None:intergenic 50.0%
GAACGCGAGAGAATTCAGGA+GGG + chr6.1:24591095-24591114 None:intergenic 50.0%
GCAAGGACACTCCTCAGATT+AGG + chr6.1:24591636-24591655 None:intergenic 50.0%
GTGTGGGTGTGTGAATTGGT+GGG - chr6.1:24591676-24591695 MS.gene054311:intron 50.0%
TCCTCAGATTAGGTGTGTCC+TGG + chr6.1:24591626-24591645 None:intergenic 50.0%
TCTGGATCTACAGCAGAACC+CGG + chr6.1:24592315-24592334 None:intergenic 50.0%
TGAAGCCTCCTTTGGATCAG+CGG + chr6.1:24592715-24592734 None:intergenic 50.0%
TTCTCCAATGCTGCCCTTTC+TGG + chr6.1:24592333-24592352 None:intergenic 50.0%
! ATTGAGCTGACGGATGAAGC+TGG + chr6.1:24591151-24591170 None:intergenic 50.0%
! GATTGGAAGGCATGGAGAAG+AGG - chr6.1:24592457-24592476 MS.gene054311:intron 50.0%
! GATTTTCAGCGGCGATTGGA+AGG - chr6.1:24592444-24592463 MS.gene054311:intron 50.0%
! GCAGTGGCTGATGATGATTG+TGG + chr6.1:24590949-24590968 None:intergenic 50.0%
! GGAAGATTTTCAGCGGCGAT+TGG - chr6.1:24592440-24592459 MS.gene054311:intron 50.0%
! TTCGGTGTGGGTGTGTGAAT+TGG - chr6.1:24591672-24591691 MS.gene054311:intron 50.0%
AACTGACCCCGCTGATCCAA+AGG - chr6.1:24592704-24592723 MS.gene054311:CDS 55.0%
AAGCCTCCTTTGGATCAGCG+GGG + chr6.1:24592713-24592732 None:intergenic 55.0%
ACCAAGCTACCTTCGGCAAC+AGG + chr6.1:24592621-24592640 None:intergenic 55.0%
AGGAAGCAAATCTGGCGGTG+GGG + chr6.1:24592028-24592047 None:intergenic 55.0%
CAGGAAGCAAATCTGGCGGT+GGG + chr6.1:24592029-24592048 None:intergenic 55.0%
CCGGAGGATGACTATGACTG+TGG + chr6.1:24592296-24592315 None:intergenic 55.0%
GAAGCCTCCTTTGGATCAGC+GGG + chr6.1:24592714-24592733 None:intergenic 55.0%
GATTAGGTGTGTCCTGGCGT+CGG + chr6.1:24591620-24591639 None:intergenic 55.0%
GCCAGGACACACCTAATCTG+AGG - chr6.1:24591622-24591641 MS.gene054311:intron 55.0%
GCTGATCCAAAGGAGGCTTC+AGG - chr6.1:24592714-24592733 MS.gene054311:CDS 55.0%
GGTGTGGGTGTGTGAATTGG+TGG - chr6.1:24591675-24591694 MS.gene054311:intron 55.0%
TCAGGAAGCAAATCTGGCGG+TGG + chr6.1:24592030-24592049 None:intergenic 55.0%
TCCAAGTCGAAGCAGCTGCT+CGG - chr6.1:24592742-24592761 MS.gene054311:CDS 55.0%
!! GAGGAGTGTCCTTGCTTCGT+AGG - chr6.1:24591641-24591660 MS.gene054311:intron 55.0%
CACATGCAGCCTGTTGCCGA+AGG - chr6.1:24592609-24592628 MS.gene054311:CDS 60.0%
GCCTGTTGCCGAAGGTAGCT+TGG - chr6.1:24592617-24592636 MS.gene054311:CDS 60.0%
GCTGCCGACGGAAGCAAAGA+AGG - chr6.1:24590967-24590986 MS.gene054311:CDS 60.0%
GGATCTACAGCAGAACCCGG+AGG + chr6.1:24592312-24592331 None:intergenic 60.0%
TCAGCGGCGATTGGAAGGCA+TGG - chr6.1:24592449-24592468 MS.gene054311:intron 60.0%
TCATCAGCCACTGCTGCCGA+CGG - chr6.1:24590955-24590974 MS.gene054311:CDS 60.0%
TGACCCCGCTGATCCAAAGG+AGG - chr6.1:24592707-24592726 MS.gene054311:CDS 60.0%
TTTGCTTCCGTCGGCAGCAG+TGG + chr6.1:24590965-24590984 None:intergenic 60.0%
GCCGAGCAGCTGCTTCGACT+TGG + chr6.1:24592746-24592765 None:intergenic 65.0%
GTCGAAGCAGCTGCTCGGCA+AGG - chr6.1:24592747-24592766 MS.gene054311:CDS 65.0%
! CGGTGTTAGACACCGACGCC+AGG - chr6.1:24591605-24591624 MS.gene054311:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 24590934 24592848 24590934 ID=MS.gene054311
chr6.1 mRNA 24590934 24592848 24590934 ID=MS.gene054311.t1;Parent=MS.gene054311
chr6.1 exon 24592606 24592848 24592606 ID=MS.gene054311.t1.exon1;Parent=MS.gene054311.t1
chr6.1 CDS 24592606 24592848 24592606 ID=cds.MS.gene054311.t1;Parent=MS.gene054311.t1
chr6.1 exon 24590934 24591590 24590934 ID=MS.gene054311.t1.exon2;Parent=MS.gene054311.t1
chr6.1 CDS 24590934 24591590 24590934 ID=cds.MS.gene054311.t1;Parent=MS.gene054311.t1
Gene Sequence

>MS.gene054311

ATGAATTCTGCACCACAATCATCATCAGCCACTGCTGCCGACGGAAGCAAAGAAGGTATGGCACCGTTTCTTCAGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTCTTCTCCCAACCTATTTCAAACACAGCAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGTAAGTCAATTCACGCTATAAGCTTCTTTTGATTATTTCATTGTCACTTTGTATGATCAATTAGTTATTGTCTTTCACTGTGTTTTTGCTTGATTGTTTGTTGTATTTGTATGATTTGGTTTCATTGTGTCTTCATCGAATTTCAATTAGTGGATACTGATTATTATGATGATCATGATCATTAAGAGATTGATGAAAAAAATGATTAGTAAGATAAGAATTAGGTACAACTTTAGATTTGGATTATTTCATTGAGTTTAGTTGGAGATACAAAATGTTGAGACATGAGAATGTTGAAAGATGGACGAGATATTATAGTCTTGTATGGCACTTTTATTCTATGAAACAGAAAACACAGACACCAGACACATCACAAACAACGAAACGCAAACACCGACAATGACATAATTCAGTATAATCATATGTGTCGGTGTTAGACACCGACGCCAGGACACACCTAATCTGAGGAGTGTCCTTGCTTCGTAGGTCTGAATTTTCGGTGTGGGTGTGTGAATTGGTGGGTAATTTAGGACTCTAAATGCACTCAAGAAAAACGATCAAAAAAATTTCTGCAGCATCTGGATCTTGGTAATGATGGTACATCGAATTATGAATAAAACCATCAATTTAGTCTCTGAATATCACACTATTTCCGGTTTAGTTCCTGAACTATATAAATATGATAAGTAGTTCCTGATTTTTATTGTTTAATCCTGCTTCTCTTCTGCATCGTAGACATCTCAAGCATCTAGGTTTTCATTTATTTAACTTGTTGAAATTGATATCTAAGTCTTAGATAGGGTTGAAAAATAGAAATCATGCACTTATGGCAATGATTAAAAACTAAGCCCCACCGCCAGATTTGCTTCCTGAAGTTTTTGTTTAATGAAGAATATTCTTGTAGAGATTTAATGGTAAATCGTTCAAAATAGGTCTTATGGATGTAATGATAAATAATTGTTTAAGAATGTATGTTTTAATTTGTTACGGTTTATTTATGTGTGCTTTTTATCAGGGATTTCGAAAATCAGATCCTGATCTTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTGCTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTCTCGCGAGAAAAATGCCCTTCAATCCAAACTCTTAAGCTATAATTATCTTGAGACAGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGAAGGCATGGAGAAGAGGCAGACAAATTTGCAGAATTTCTTTGAGAAGGCTCTTCAGGATTCTTTCATTGTTGAACTTCTTTCCCGCAAAATCGAATCCATGGATTTGGAAACTTATAATAAGAAAAGGCGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCTTGGTTGACCTTCCTAGCAATTTCTCTCAGAATCTTTCTGAAGGAGAACTGACAGGAATACAGACAAGAACTGACCCCGCTGATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCAAGGAGTGAATGATGTGTTTTGGGAACAATTCCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGAAATGTTCAACTAA
Protein sequence

>MS.gene054311.t1

MNSAPQSSSATAADGSKEGMAPFLQKTYDMVDDPTTDEIVSWSSDNKSFIVWNPPEFSRVLLPTYFKHSNFSSFIRQLNTYGFRKSDPDLWEFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSAVDPERAALEKEIEKLSREKNALQSKLLSYNYLETEKLQLEDFQRRLEGMEKRQTNLQNFFEKALQDSFIVELLSRKIESMDLETYNKKRRLSQVDHMQPVAEGSLVDLPSNFSQNLSEGELTGIQTRTDPADPKEASGNVQVEAAARQGVNDVFWEQFLTESPCCSDNEEEMFN