AlfalfaGEDB | MS.gene054312

M. sativa cultivar XinJiangDaYe / MS.gene054312

KEGG: K09419
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054312.t1	XP_003629611.1	59.1	457	53	5	1	323	1	457	2.40E-124	455.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054312.t1	Q94BZ5	40.7	432	143	7	1	319	1	432	4.4e-71	269.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054312.t1	G7LH28	59.1	457	53	5	1	323	1	457	1.7e-124	455.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene054312.t1	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054312.t1	MTR_6g043060	72.590	332	75	5	1	321	1	327	1.47e-168	471
MS.gene054312.t1	MTR_8g083100	76.871	294	29	4	23	277	23	316	4.01e-153	438
MS.gene054312.t1	MTR_8g083100	78.261	46	10	0	278	323	412	457	9.66e-16	78.2
MS.gene054312.t1	MTR_2g100680	58.228	316	90	6	1	276	12	325	6.17e-116	342
MS.gene054312.t1	MTR_6g044340	62.222	270	74	3	68	323	8	263	7.68e-110	320
MS.gene054312.t1	MTR_2g100670	56.446	287	101	5	1	269	14	294	1.04e-99	296
MS.gene054312.t1	MTR_2g100670	56.446	287	101	5	1	269	122	402	8.46e-98	295
MS.gene054312.t1	MTR_5g029680	51.185	211	89	4	22	218	32	242	7.17e-64	210
MS.gene054312.t1	MTR_8g087540	36.915	363	159	9	15	319	5	355	9.78e-64	207
MS.gene054312.t1	MTR_6g044430	51.383	253	49	6	100	319	14	225	1.64e-62	198

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054312.t1	AT4G13980	56.710	231	98	2	1	229	1	231	6.65e-85	263
MS.gene054312.t1	AT4G17750	49.074	216	89	4	22	216	52	267	6.75e-60	199
MS.gene054312.t1	AT1G32330	47.234	235	105	5	1	216	16	250	2.58e-58	195
MS.gene054312.t1	AT1G32330	47.234	235	105	5	1	216	16	250	2.61e-58	195
MS.gene054312.t1	AT5G16820	47.345	226	101	6	20	228	25	249	1.01e-57	193
MS.gene054312.t1	AT5G16820	47.345	226	101	6	20	228	25	249	1.01e-57	193
MS.gene054312.t1	AT3G02990	42.918	233	120	4	8	228	9	240	1.37e-56	190
MS.gene054312.t1	AT4G18880	48.341	211	99	3	16	220	9	215	1.58e-51	175
MS.gene054312.t1	AT5G45710	36.025	322	171	8	14	319	5	307	8.41e-50	169
MS.gene054312.t1	AT5G45710	36.025	322	171	8	14	319	5	307	8.41e-50	169

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Find 73 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGATCCTGATCGTT+GGG	0.473476	6.1:-24573630	MS.gene054312:CDS
AAATAAACTCCCTGAAGATG+AGG	0.580535	6.1:-24573159	MS.gene054312:CDS
AACTGACCAAGCAGTTACTG+GGG	0.698051	6.1:-24573084	MS.gene054312:CDS
AAGATGCCCCAGTAACTGCT+TGG	0.594092	6.1:+24573078	None:intergenic
AAGGTAGCTTGGTTAATCCA+GGG	0.645414	6.1:-24573202	MS.gene054312:CDS
ACAGAGTGAATGATGTGTTT+TGG	0.360172	6.1:-24572992	MS.gene054312:CDS
ACCAAGCTACCTTCGGCAAC+AGG	0.417304	6.1:+24573212	None:intergenic
ACGTTACCTGAAGCCTCCTT+TGG	0.398346	6.1:+24573038	None:intergenic
AGAAACGATCTCATCTGTGG+TGG	0.582151	6.1:+24574786	None:intergenic
AGAACGCGAGAGAATTCAGG+AGG	0.628084	6.1:+24574727	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAATTTCTGCAAGCATC+TGG	-	chr6.1:24573703-24573722	MS.gene054312:intron	30.0%
AGATCAAAAACATCTTCTGA+CGG	-	chr6.1:24574236-24574255	MS.gene054312:intron	30.0%
ATAACAAAAGCTTCATCGTT+TGG	-	chr6.1:24573065-24573084	MS.gene054312:CDS	30.0%
ATAATCATACATGTCGTTGT+CGG	-	chr6.1:24573557-24573576	MS.gene054312:CDS	30.0%
ATATAGTTCAGGAACTAAAC+CGG	+	chr6.1:24573799-24573818	None:intergenic	30.0%
CAAACAATCAAGCAAAAACA+CGG	+	chr6.1:24573255-24573274	None:intergenic	30.0%
CTTCATCGAATTTCAATTAG+TGG	-	chr6.1:24573305-24573324	MS.gene054312:CDS	30.0%
CTTCATTACACAAAAACTTC+AGG	+	chr6.1:24574029-24574048	None:intergenic	30.0%
TCTGAATATCACACTATTTC+CGG	-	chr6.1:24573777-24573796	MS.gene054312:intron	30.0%
AAAACTTCAGGAAGCAAATC+TGG	+	chr6.1:24574017-24574036	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	24572932	24574908	24572932	ID=MS.gene054312
chr6.1	mRNA	24572932	24574908	24572932	ID=MS.gene054312.t1;Parent=MS.gene054312
chr6.1	exon	24574666	24574908	24574666	ID=MS.gene054312.t1.exon1;Parent=MS.gene054312.t1
chr6.1	CDS	24574666	24574908	24574666	ID=cds.MS.gene054312.t1;Parent=MS.gene054312.t1
chr6.1	exon	24572932	24573660	24572932	ID=MS.gene054312.t1.exon2;Parent=MS.gene054312.t1
chr6.1	CDS	24572932	24573660	24572932	ID=cds.MS.gene054312.t1;Parent=MS.gene054312.t1

Gene Sequence

>MS.gene054312

ATGAATTCTGCACCACAATCATCATCAGCCGCTGCTGCCGACGGAAGCAGAGAAGGTATGGCACCGTTTCTTCAGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCCCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTCTTCTCCCAACCTATTTCAAACACAACAATTTCTCCAGCTTCATCCGTCAGCTTAATACCTACGTAAGTCAATTCATGCTATAAGCTTCTTTTGATTATTTCATTGTTACTTTGTATGATCAATTAGTTATTGTCTTTCACCGTGTTTTTGCTTGATTGTTTGTTGTATTTGTATGATTTGGTTTCATTGTGTCTTCATCGAATTTCAATTAGTGGATATTGATTATTATGATGATAGTGATCATTAAGAGATTGATGAAAAAAATGATTAGTAAGATAAGAATTAGGTACAACTTTAGATTTGGATTATTTCACTGAGTTTAGTTGGAGATACAAAATTTTGAAACCTGAGAATGTTGAAAGATGGATGAGAAATTATAGTGTTGTATGGCACTTTTATTCTATGAAACATAAAACAGAGACACCGACAATGATATAATTCAATATAATCATACATGTCGTTGTCGGACACCGACGCCATGACACACCTAATCCAAGGAGTGTCCTTGCTTCGTAGGTCTTAATTTTTGGTGTGGGTGTGTGAATTGGTGGGTGATTTAGGACTCTAAATGCACTCAAGAAAAACAATCAAAAAAATTTCTGCAAGCATCTGGATCTTGGTAATGATGGTACATCGAATCATGAATAAAACCATCAATTTAGTCTCTGAATATCACACTATTTCCGGTTTAGTTCCTGAACTATATCAATATGATAAGTAGTTTCTAAAGTATATGAAATTTGTAGTTCCTGATTTTTATTGTTTAATTCTGCATCGTACACATCTCAAGCATCTAGGTTTTCATTTATTGAACTTGTTGAAATTGATATCTAAGTCTTAGATAGGGTTGAAAAATAGAAATCATGCACTTATGGCAATGATTAAAAACTAAGCCCCACCGCCAGATTTGCTTCCTGAAGTTTTTGTGTAATGAAGAATATTCTTGTAGAGATTTAATAGTAAATCGTTCAAAAAAGTTTGTAGGTCTTATGGTTGTAATGATAAATAATTGTTTAAGAATGTATGTTTTAATTTGTTACGGTTTATTTACGTGTGCTTTTTATCAGGGATTTCGAAAATCAGATCCTGATCGTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTACTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTTTCGCGCGAAAAATGCCCTTCAATCCAAACTCCTAAGCTATAATTATCTTGAGACTGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGATGGCATGGAGAAGAGGCAGACAAATTTGCAGAACTTCTTTGAGAAGGCTCTTCAAGATTCTTTTATTGTTGAACTTCTTTCCCACAAAATCGAATCCATGGATTTGGAAACTTATAATAAGAAAAGGCGATTGTCTCAGGTTGATCGCACGCAGCCTGTTGCCGAAGGTAGCTTGGTTAATCCAGGGAATATTGCCGAAGATATCTCAAATAAACTCCCTGAAGATGAGGAAAGCCTGCAGAAAAATCTTTCTGACGGAGAACTGATAGGAATGCAGACAAGAACTGACCAAGCAGTTACTGGGGCATCTTTTACTAATGTGGCTAATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCACAGAGTGAATGATGTGTTTTGGGAACAATTTCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGAAATGTTCAACTAA

Protein sequence

>MS.gene054312.t1

MNSAPQSSSAAAADGSREGMAPFLQKTYDMVDDPTTDEIVSWSPDNKSFIVWNPPEFSRVLLPTYFKHNNFSSFIRQLNTYGFRKSDPDRWEFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSTVDPERAALEKEIEKLFRAKNALQSKLLSYNYLETEKLQLEDFQRRLDGMEKRQTNLQNFFEKALQDSFIVELLSHKIESMDLETYNKKRRLSQVDRTQPVAEGSLVNPGNIAEDISNKLPEDEESLQKNLSDGELIGMQTRTDQAVTGASFTNVANPKEASGNVQVEAAARHRVNDVFWEQFLTESPCCSDNEEEMFN