AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05446

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05446.t1 MTR_6g084120 93.625 251 4 2 1 251 25 263 9.91e-177 486
MS.gene05446.t1 MTR_7g053410 60.909 110 43 0 105 214 288 397 1.94e-37 137
MS.gene05446.t1 MTR_7g053410 60.909 110 43 0 105 214 288 397 3.56e-37 137
MS.gene05446.t1 MTR_8g012290 62.136 103 39 0 112 214 269 371 7.81e-36 133
MS.gene05446.t1 MTR_8g012290 62.136 103 39 0 112 214 269 371 8.29e-36 133
MS.gene05446.t1 MTR_5g037250 55.963 109 41 1 105 206 76 184 8.22e-35 125
MS.gene05446.t1 MTR_3g498825 64.894 94 32 1 125 217 138 231 1.08e-34 127
MS.gene05446.t1 MTR_8g065740 58.559 111 44 1 103 213 276 384 5.19e-34 127
MS.gene05446.t1 MTR_5g048860 59.633 109 42 1 106 214 339 445 6.55e-34 128
MS.gene05446.t1 MTR_4g070320 71.951 82 23 0 130 211 139 220 1.32e-33 124
MS.gene05446.t1 MTR_3g116770 45.752 153 70 2 76 217 122 272 1.91e-33 125
MS.gene05446.t1 MTR_3g116770 45.752 153 70 2 76 217 122 272 2.04e-33 125
MS.gene05446.t1 MTR_8g062240 41.146 192 70 6 68 220 60 247 2.24e-33 122
MS.gene05446.t1 MTR_8g099880 62.766 94 34 1 125 217 136 229 2.42e-33 123
MS.gene05446.t1 MTR_1g017350 60.000 100 39 1 115 213 240 339 1.18e-32 123
MS.gene05446.t1 MTR_1g017350 60.000 100 39 1 115 213 240 339 1.19e-32 123
MS.gene05446.t1 MTR_1g017350 60.000 100 39 1 115 213 240 339 1.23e-32 123
MS.gene05446.t1 MTR_1g017350 60.000 100 39 1 115 213 240 339 1.29e-32 123
MS.gene05446.t1 MTR_1g059270 67.500 80 26 0 132 211 158 237 9.57e-32 117
MS.gene05446.t1 MTR_1g059270 67.500 80 26 0 132 211 158 237 2.37e-31 118
MS.gene05446.t1 MTR_7g092510 67.500 80 26 0 132 211 171 250 1.38e-30 116
MS.gene05446.t1 MTR_4g131160 62.121 66 24 1 134 199 196 260 1.14e-17 81.6
MS.gene05446.t1 MTR_4g131160 62.121 66 24 1 134 199 199 263 1.24e-17 81.6
MS.gene05446.t1 MTR_1g080890 59.091 66 26 1 134 199 193 257 9.91e-17 79.0
MS.gene05446.t1 MTR_1g069155 45.570 79 42 1 121 199 304 381 3.03e-14 72.0
MS.gene05446.t1 MTR_5g005110 43.529 85 47 1 114 198 204 287 2.16e-13 68.9
MS.gene05446.t1 MTR_1g019240 44.304 79 43 1 122 200 73 150 2.42e-13 68.2
MS.gene05446.t1 MTR_4g033295 49.231 65 33 0 134 198 118 182 4.90e-13 66.2
MS.gene05446.t1 MTR_4g033295 47.761 67 34 1 134 199 118 184 7.47e-13 65.5
MS.gene05446.t1 MTR_1g018090 50.769 65 31 1 134 198 274 337 8.07e-13 67.8
MS.gene05446.t1 MTR_4g065870 49.231 65 33 0 134 198 355 419 1.01e-12 67.4
MS.gene05446.t1 MTR_3g103030 44.318 88 44 2 111 198 210 292 3.14e-12 65.5
MS.gene05446.t1 MTR_1g070870 57.576 66 27 1 134 199 120 184 3.47e-12 65.1
MS.gene05446.t1 MTR_1g070870 57.576 66 27 1 134 199 123 187 3.88e-12 65.1
MS.gene05446.t1 MTR_2g098310 46.154 65 35 0 134 198 332 396 4.11e-12 65.5
MS.gene05446.t1 MTR_4g065870 48.387 62 32 0 134 195 355 416 6.50e-12 65.1
MS.gene05446.t1 MTR_7g099540 43.038 79 43 2 121 199 240 316 7.02e-12 65.1
MS.gene05446.t1 MTR_2g098310 46.875 64 34 0 134 197 332 395 1.34e-11 64.3
MS.gene05446.t1 MTR_7g092540 46.269 67 36 0 134 200 243 309 2.27e-11 63.2
MS.gene05446.t1 MTR_1g084980 45.312 64 34 1 136 199 464 526 2.29e-11 63.9
MS.gene05446.t1 MTR_7g099540 43.038 79 43 2 121 199 240 316 4.64e-11 62.8
MS.gene05446.t1 MTR_3g449770 35.345 116 54 3 82 197 277 371 8.63e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05446.t1 AT1G73830 52.632 247 80 10 21 251 36 261 5.94e-65 203
MS.gene05446.t1 AT1G73830 52.632 247 79 11 21 251 36 260 7.21e-63 197
MS.gene05446.t1 AT1G18400 78.704 108 23 0 131 238 142 249 1.57e-59 189
MS.gene05446.t1 AT1G18400 84.615 91 14 0 131 221 142 232 2.48e-53 173
MS.gene05446.t1 AT1G25330 64.407 118 41 1 124 240 94 211 1.72e-47 157
MS.gene05446.t1 AT5G50915 62.264 106 39 1 113 217 115 220 4.46e-39 137
MS.gene05446.t1 AT5G50915 62.264 106 39 1 113 217 115 220 4.46e-39 137
MS.gene05446.t1 AT5G50915 62.264 106 39 1 113 217 115 220 8.20e-39 137
MS.gene05446.t1 AT5G50915 62.264 106 39 1 113 217 115 220 8.20e-39 137
MS.gene05446.t1 AT3G07340 58.197 122 45 1 96 211 214 335 3.69e-37 135
MS.gene05446.t1 AT3G07340 58.197 122 45 1 96 211 214 335 4.18e-37 136
MS.gene05446.t1 AT5G48560 70.115 87 25 1 126 211 293 379 1.01e-34 129
MS.gene05446.t1 AT5G48560 70.115 87 25 1 126 211 293 379 1.66e-34 129
MS.gene05446.t1 AT1G26260 69.880 83 25 0 132 214 170 252 3.49e-34 125
MS.gene05446.t1 AT1G26260 69.880 83 25 0 132 214 199 281 8.31e-34 125
MS.gene05446.t1 AT1G26260 69.880 83 25 0 132 214 221 303 1.00e-33 125
MS.gene05446.t1 AT1G26260 69.880 83 25 0 132 214 221 303 1.00e-33 125
MS.gene05446.t1 AT1G26260 69.880 83 25 0 132 214 221 303 1.00e-33 125
MS.gene05446.t1 AT1G26260 69.880 83 25 0 132 214 221 303 1.00e-33 125
MS.gene05446.t1 AT1G68920 71.951 82 23 0 132 213 300 381 1.52e-33 127
MS.gene05446.t1 AT3G23690 65.517 87 30 0 126 212 183 269 1.63e-33 125
MS.gene05446.t1 AT1G68920 71.951 82 23 0 132 213 301 382 1.63e-33 126
MS.gene05446.t1 AT1G68920 71.951 82 23 0 132 213 301 382 1.63e-33 126
MS.gene05446.t1 AT1G68920 71.951 82 23 0 132 213 301 382 1.63e-33 126
MS.gene05446.t1 AT1G59640 67.901 81 26 0 126 206 128 208 2.24e-33 122
MS.gene05446.t1 AT4G34530 62.105 95 34 1 123 217 161 253 1.13e-32 122
MS.gene05446.t1 AT4G36540 61.386 101 36 2 122 221 128 226 3.47e-32 120
MS.gene05446.t1 AT4G36540 61.386 101 36 2 122 221 128 226 3.58e-32 120
MS.gene05446.t1 AT3G57800 63.736 91 33 0 124 214 194 284 5.42e-32 120
MS.gene05446.t1 AT1G59640 64.444 90 27 1 122 206 119 208 5.99e-32 120
MS.gene05446.t1 AT5G62610 69.737 76 23 0 126 201 145 220 7.34e-32 118
MS.gene05446.t1 AT5G62610 69.737 76 23 0 126 201 145 220 7.34e-32 118
MS.gene05446.t1 AT1G10120 60.825 97 36 1 119 215 191 285 1.54e-31 119
MS.gene05446.t1 AT1G10120 60.825 97 36 1 119 215 191 285 1.54e-31 119
MS.gene05446.t1 AT2G42300 66.250 80 27 0 132 211 183 262 1.07e-30 115
MS.gene05446.t1 AT2G42300 49.593 123 52 3 132 249 183 300 3.22e-30 115
MS.gene05446.t1 AT2G18300 55.455 110 41 1 102 203 145 254 1.32e-29 114
MS.gene05446.t1 AT2G18300 55.455 110 41 1 102 203 145 254 1.32e-29 114
MS.gene05446.t1 AT2G18300 55.455 110 41 1 102 203 145 254 2.62e-29 113
MS.gene05446.t1 AT3G57800 42.029 138 33 1 124 214 194 331 3.42e-23 97.8
MS.gene05446.t1 AT2G24260 54.321 81 35 2 134 213 35 114 7.02e-18 80.5
MS.gene05446.t1 AT4G30980 62.121 66 24 1 134 199 130 194 1.41e-17 80.9
MS.gene05446.t1 AT4G30980 62.121 66 24 1 134 199 178 242 1.67e-17 81.3
MS.gene05446.t1 AT5G58010 54.545 77 34 1 123 199 88 163 2.68e-17 79.7
MS.gene05446.t1 AT2G24260 54.321 81 35 2 134 213 138 217 3.35e-17 80.1
MS.gene05446.t1 AT2G42300 75.610 41 10 0 132 172 183 223 2.95e-14 70.1
MS.gene05446.t1 AT2G42300 75.610 41 10 0 132 172 183 223 2.95e-14 70.1
MS.gene05446.t1 AT2G42300 75.610 41 10 0 132 172 183 223 3.94e-14 70.1
MS.gene05446.t1 AT1G09530 36.552 145 77 5 74 208 267 406 2.11e-13 69.7
MS.gene05446.t1 AT1G09530 36.552 145 77 5 74 208 267 406 2.11e-13 69.7
MS.gene05446.t1 AT1G09530 36.552 145 77 5 74 208 267 406 2.11e-13 69.7
MS.gene05446.t1 AT1G09530 36.552 145 77 5 74 208 267 406 2.11e-13 69.7
MS.gene05446.t1 AT1G09530 36.552 145 77 5 74 208 267 406 2.11e-13 69.7
MS.gene05446.t1 AT1G09530 36.552 145 77 5 74 208 267 406 2.11e-13 69.7
MS.gene05446.t1 AT2G20180 42.857 77 43 1 123 199 196 271 2.64e-13 69.3
MS.gene05446.t1 AT2G20180 42.857 77 43 1 123 199 196 271 2.64e-13 69.3
MS.gene05446.t1 AT2G20180 42.857 77 43 1 123 199 196 271 2.64e-13 69.3
MS.gene05446.t1 AT2G20180 42.857 77 43 1 123 199 267 342 2.77e-13 69.3
MS.gene05446.t1 AT2G20180 42.857 77 43 1 123 199 267 342 2.77e-13 69.3
MS.gene05446.t1 AT2G20180 42.857 77 43 1 123 199 267 342 2.77e-13 69.3
MS.gene05446.t1 AT2G20180 42.857 77 43 1 123 199 267 342 2.77e-13 69.3
MS.gene05446.t1 AT1G27740 32.258 155 81 5 67 198 77 230 4.91e-13 67.4
MS.gene05446.t1 AT4G33880 40.496 121 63 4 82 198 214 329 8.23e-13 67.4
MS.gene05446.t1 AT2G46970 40.964 83 43 2 122 199 206 287 1.51e-12 67.0
MS.gene05446.t1 AT2G46970 40.964 83 43 2 122 199 184 265 1.83e-12 66.6
MS.gene05446.t1 AT2G46970 40.964 83 43 2 122 199 175 256 1.93e-12 66.6
MS.gene05446.t1 AT2G20180 43.243 74 41 1 123 196 267 339 2.95e-12 65.9
MS.gene05446.t1 AT1G03040 49.383 81 39 2 134 213 139 218 7.61e-12 64.3
MS.gene05446.t1 AT1G03040 49.383 81 39 2 134 213 144 223 9.25e-12 64.3
MS.gene05446.t1 AT4G02590 56.061 66 28 1 134 199 83 147 1.20e-11 63.2
MS.gene05446.t1 AT4G02590 56.061 66 28 1 134 199 146 210 1.41e-11 63.5
MS.gene05446.t1 AT4G02590 56.061 66 28 1 134 199 146 210 1.41e-11 63.5
MS.gene05446.t1 AT1G03040 49.383 81 39 2 134 213 144 223 1.57e-11 63.5
MS.gene05446.t1 AT4G00050 42.683 82 46 1 117 198 88 168 1.80e-11 63.2
MS.gene05446.t1 AT1G35460 44.776 67 37 0 132 198 179 245 2.37e-11 62.8
MS.gene05446.t1 AT4G28790 33.981 103 65 2 99 199 234 335 2.60e-11 63.5
MS.gene05446.t1 AT4G09180 45.455 66 36 0 133 198 183 248 2.73e-11 62.4
MS.gene05446.t1 AT4G28815 36.585 82 47 2 137 214 71 151 4.02e-11 61.6
MS.gene05446.t1 AT4G00050 45.333 75 40 1 124 198 197 270 4.12e-11 62.8
MS.gene05446.t1 AT4G28800 40.244 82 44 2 117 198 236 312 4.43e-11 62.8
MS.gene05446.t1 AT4G00050 45.333 75 40 1 124 198 197 270 4.90e-11 62.4
MS.gene05446.t1 AT2G43140 48.485 66 34 0 132 197 132 197 5.85e-11 60.8
MS.gene05446.t1 AT2G43140 50.000 64 32 0 134 197 231 294 9.21e-11 61.2

Find 35 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGAAGAAATTTACAAGTT+TGG 0.242755 6.4:-58085207 None:intergenic
TATTGTTCCAGGATGTTATA+AGG 0.296431 6.4:+58085873 MS.gene05446:CDS
AAGATGTTGTCACAAGAAAA+TGG 0.334761 6.4:-58084945 None:intergenic
TGCTTGCAAAATATTGTTCC+AGG 0.400546 6.4:+58085862 MS.gene05446:CDS
ACTCCTGCAGTTTCTGAAAG+TGG 0.440216 6.4:+58085176 MS.gene05446:CDS
GGAACTATTGAATCAATTCA+TGG 0.446503 6.4:+58084887 MS.gene05446:CDS
ACATGTGTTTCCAAGAAACT+TGG 0.456545 6.4:+58084995 MS.gene05446:CDS
CTTCCACTTTCAGAAACTGC+AGG 0.466079 6.4:-58085179 None:intergenic
TTGCTTGCTTTCAACCAACT+TGG 0.490280 6.4:+58086853 MS.gene05446:CDS
TAGCAAGGTATAAGAGAGAA+GGG 0.498236 6.4:+58086820 MS.gene05446:CDS
GAAAATAAAGTTCATCATGA+AGG 0.498927 6.4:+58085104 MS.gene05446:CDS
TTGATTTGTGGCAGAGAGCA+AGG 0.504432 6.4:+58086778 MS.gene05446:intron
TTTCAATGGTTGCAGACCAT+GGG 0.515237 6.4:+58085985 MS.gene05446:intron
AGGTATAAGAGAGAAGGGTA+TGG 0.517402 6.4:+58086825 MS.gene05446:CDS
TTAGCAAGGTATAAGAGAGA+AGG 0.520461 6.4:+58086819 MS.gene05446:CDS
TGAAGTCAAAGTGGCCAAGT+TGG 0.520695 6.4:-58086867 None:intergenic
TAATTTCAGAGTGGTGGAAG+AGG 0.521842 6.4:+58085312 MS.gene05446:intron
TATGATTAATTTCAGAGTGG+TGG 0.522541 6.4:+58085306 MS.gene05446:intron
GACTGTGACTATCAGTAGCT+TGG 0.523846 6.4:-58085407 None:intergenic
TAGTCACAGTCTAGCAGAAA+GGG 0.525871 6.4:+58085419 MS.gene05446:CDS
TATAAGAGAGAAGGGTATGG+AGG 0.527385 6.4:+58086828 MS.gene05446:CDS
CATGGGAATGGCAGTAATGT+TGG 0.527745 6.4:+58086002 MS.gene05446:CDS
ATATTAACCTTATAACATCC+TGG 0.539111 6.4:-58085880 None:intergenic
TTGTTGTTCAGGTTAGAAGA+GGG 0.558240 6.4:+58085818 MS.gene05446:intron
ATAGTCACAGTCTAGCAGAA+AGG 0.561228 6.4:+58085418 MS.gene05446:CDS
ATCCAAAATTGAAGTCAAAG+TGG 0.565182 6.4:-58086876 None:intergenic
GCAGAGAGCAAGGGCATCTG+AGG 0.566936 6.4:+58086788 MS.gene05446:intron
TGATTTGTGGCAGAGAGCAA+GGG 0.569667 6.4:+58086779 MS.gene05446:intron
TTTGTTGTTCAGGTTAGAAG+AGG 0.588572 6.4:+58085817 MS.gene05446:intron
ATGGTTGCAGACCATGGGAA+TGG 0.592994 6.4:+58085990 MS.gene05446:intron
GTTCATGTTAGAGCAAGAAG+AGG 0.594328 6.4:+58085384 MS.gene05446:CDS
CTGAGGCAAAAGAGTTAGCA+AGG 0.595828 6.4:+58086805 MS.gene05446:CDS
AACAGAAGAAGAGAAAGCAA+AGG 0.600831 6.4:+58085356 MS.gene05446:CDS
CAACATTACTGCCATTCCCA+TGG 0.603267 6.4:-58086001 None:intergenic
AGATGATCTAGAAACAATGC+AGG 0.618754 6.4:+58086262 MS.gene05446:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTTTTAAATTAAAA+AGG - chr6.4:58085911-58085930 None:intergenic 0.0%
!!! GTTAATTTTTTTTAAAAGAA+AGG + chr6.4:58086524-58086543 MS.gene05446:intron 10.0%
!!! TAAACTATTTCTATAATAAA+TGG + chr6.4:58085511-58085530 MS.gene05446:intron 10.0%
!!! TTATTTATTTATTTGATTTG+TGG + chr6.4:58086766-58086785 MS.gene05446:intron 10.0%
!! ATATTATTATATGAATTGAG+AGG - chr6.4:58086386-58086405 None:intergenic 15.0%
!! GATGAAATTAAATTATTAAC+TGG + chr6.4:58085269-58085288 MS.gene05446:intron 15.0%
!! TATTAATGTCAATTTATGTT+TGG + chr6.4:58086171-58086190 MS.gene05446:intron 15.0%
!!! AATCAAATTGGTTTTATATA+AGG + chr6.4:58085542-58085561 MS.gene05446:intron 15.0%
!!! AGTAATATTCATTTATTTCT+AGG + chr6.4:58086072-58086091 MS.gene05446:intron 15.0%
!!! CACTAATTATAATTTTTTTG+AGG - chr6.4:58086141-58086160 None:intergenic 15.0%
!!! TTATTTTGTTTTTGTTTCAA+TGG + chr6.4:58085971-58085990 MS.gene05446:intron 15.0%
!! AAGTTAAAATTCATTAGAGT+TGG + chr6.4:58086427-58086446 MS.gene05446:intron 20.0%
!! ATATAATATGCTCATATTCA+TGG + chr6.4:58086322-58086341 MS.gene05446:intron 20.0%
!! ATGGTAAAATCAAATCAAAT+TGG + chr6.4:58085530-58085549 MS.gene05446:intron 20.0%
!!! AAATATTTGTTTTGTTGTTC+AGG + chr6.4:58085807-58085826 MS.gene05446:intron 20.0%
!!! ATCTATTTCTAAAAGTTTCA+TGG + chr6.4:58086289-58086308 MS.gene05446:intron 20.0%
! AGGTAAAAAGAGAAAAATGA+TGG + chr6.4:58085124-58085143 MS.gene05446:CDS 25.0%
! ATATTAACCTTATAACATCC+TGG - chr6.4:58085883-58085902 None:intergenic 25.0%
! GAAAATAAAGTTCATCATGA+AGG + chr6.4:58085104-58085123 MS.gene05446:CDS 25.0%
! GTAGAAGAAATTTACAAGTT+TGG - chr6.4:58085210-58085229 None:intergenic 25.0%
! TTCTATGATTAATTTCAGAG+TGG + chr6.4:58085303-58085322 MS.gene05446:intron 25.0%
!! AAATTTTCTTCCAAGTTTCT+TGG - chr6.4:58085008-58085027 None:intergenic 25.0%
!! AACTTGGAAGAAAATTTTCA+TGG + chr6.4:58085011-58085030 MS.gene05446:CDS 25.0%
!! TTTTCTTCTTGGAAAATTGT+TGG - chr6.4:58085089-58085108 None:intergenic 25.0%
!!! GATGAACTTTATTTTCTTCT+TGG - chr6.4:58085100-58085119 None:intergenic 25.0%
!!! GATTTTGATTATTGACATGA+TGG - chr6.4:58085043-58085062 None:intergenic 25.0%
AAGATGTTGTCACAAGAAAA+TGG - chr6.4:58084948-58084967 None:intergenic 30.0%
GGAACTATTGAATCAATTCA+TGG + chr6.4:58084887-58084906 MS.gene05446:CDS 30.0%
TAGTCCTAAAGAACCAAAAT+TGG - chr6.4:58086631-58086650 None:intergenic 30.0%
TATGATTAATTTCAGAGTGG+TGG + chr6.4:58085306-58085325 MS.gene05446:intron 30.0%
TATTGTTCCAGGATGTTATA+AGG + chr6.4:58085873-58085892 MS.gene05446:CDS 30.0%
TTGGACAAAGTTTATGTAGT+TGG - chr6.4:58086600-58086619 None:intergenic 30.0%
TTGTTCATGATTGATGTGTT+TGG + chr6.4:58085769-58085788 MS.gene05446:intron 30.0%
! ATGGTTTCTTTCATTTTGCA+TGG + chr6.4:58086341-58086360 MS.gene05446:intron 30.0%
! ATTTTCTTGTGACAACATCT+TGG + chr6.4:58084947-58084966 MS.gene05446:CDS 30.0%
! TTCATTTTGCATGGTTGTTA+GGG + chr6.4:58086350-58086369 MS.gene05446:intron 30.0%
! TTTCATTTTGCATGGTTGTT+AGG + chr6.4:58086349-58086368 MS.gene05446:intron 30.0%
!! ACCAAAATTGGTGCTAAAAT+TGG - chr6.4:58086619-58086638 None:intergenic 30.0%
!! TCCAATTTTAGCACCAATTT+TGG + chr6.4:58086615-58086634 MS.gene05446:intron 30.0%
AACAGAAGAAGAGAAAGCAA+AGG + chr6.4:58085356-58085375 MS.gene05446:CDS 35.0%
ACATGTGTTTCCAAGAAACT+TGG + chr6.4:58084995-58085014 MS.gene05446:CDS 35.0%
AGATGATCTAGAAACAATGC+AGG + chr6.4:58086262-58086281 MS.gene05446:CDS 35.0%
ATATACGCTAACTGTTGTGA+TGG + chr6.4:58086402-58086421 MS.gene05446:intron 35.0%
TAGCAAGGTATAAGAGAGAA+GGG + chr6.4:58086820-58086839 MS.gene05446:CDS 35.0%
TGCTTGCAAAATATTGTTCC+AGG + chr6.4:58085862-58085881 MS.gene05446:CDS 35.0%
TTAGCAAGGTATAAGAGAGA+AGG + chr6.4:58086819-58086838 MS.gene05446:CDS 35.0%
! TTGTTGTTCAGGTTAGAAGA+GGG + chr6.4:58085818-58085837 MS.gene05446:intron 35.0%
!! GATTTTCAAGAGACTAGTTC+TGG + chr6.4:58085146-58085165 MS.gene05446:CDS 35.0%
!! TTTGTTGTTCAGGTTAGAAG+AGG + chr6.4:58085817-58085836 MS.gene05446:intron 35.0%
!!! AGCACCAATTTTGGTTCTTT+AGG + chr6.4:58086624-58086643 MS.gene05446:intron 35.0%
AGGTATAAGAGAGAAGGGTA+TGG + chr6.4:58086825-58086844 MS.gene05446:CDS 40.0%
ATAGTCACAGTCTAGCAGAA+AGG + chr6.4:58085418-58085437 MS.gene05446:CDS 40.0%
GTTCATGTTAGAGCAAGAAG+AGG + chr6.4:58085384-58085403 MS.gene05446:CDS 40.0%
TAGTCACAGTCTAGCAGAAA+GGG + chr6.4:58085419-58085438 MS.gene05446:CDS 40.0%
TTGCTTGCTTTCAACCAACT+TGG + chr6.4:58086853-58086872 MS.gene05446:CDS 40.0%
TTTCAATGGTTGCAGACCAT+GGG + chr6.4:58085985-58086004 MS.gene05446:intron 40.0%
! TAATTTCAGAGTGGTGGAAG+AGG + chr6.4:58085312-58085331 MS.gene05446:intron 40.0%
! TATAAGAGAGAAGGGTATGG+AGG + chr6.4:58086828-58086847 MS.gene05446:CDS 40.0%
!! GTTGTTTTCGTAAGCTACAC+TGG + chr6.4:58085570-58085589 MS.gene05446:intron 40.0%
CAACATTACTGCCATTCCCA+TGG - chr6.4:58086004-58086023 None:intergenic 45.0%
CATGGGAATGGCAGTAATGT+TGG + chr6.4:58086002-58086021 MS.gene05446:CDS 45.0%
CTGAGGCAAAAGAGTTAGCA+AGG + chr6.4:58086805-58086824 MS.gene05446:CDS 45.0%
CTTCCACTTTCAGAAACTGC+AGG - chr6.4:58085182-58085201 None:intergenic 45.0%
GACTGTGACTATCAGTAGCT+TGG - chr6.4:58085410-58085429 None:intergenic 45.0%
GTTTCAATGGTTGCAGACCA+TGG + chr6.4:58085984-58086003 MS.gene05446:intron 45.0%
! ACTCCTGCAGTTTCTGAAAG+TGG + chr6.4:58085176-58085195 MS.gene05446:CDS 45.0%
! TGATTTGTGGCAGAGAGCAA+GGG + chr6.4:58086779-58086798 MS.gene05446:intron 45.0%
!! TTGATTTGTGGCAGAGAGCA+AGG + chr6.4:58086778-58086797 MS.gene05446:intron 45.0%
!! TTATATTTAGTAATATTTAT+AGG + chr6.4:58086502-58086521 MS.gene05446:intron 5.0%
!!! TTAAAAAACTTTTTTTTAAA+AGG - chr6.4:58086673-58086692 None:intergenic 5.0%
ATGGTTGCAGACCATGGGAA+TGG + chr6.4:58085990-58086009 MS.gene05446:intron 50.0%
!! GCAGAGAGCAAGGGCATCTG+AGG + chr6.4:58086788-58086807 MS.gene05446:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 58084885 58086884 58084885 ID=MS.gene05446
chr6.4 mRNA 58084885 58086884 58084885 ID=MS.gene05446.t1;Parent=MS.gene05446
chr6.4 exon 58084885 58085214 58084885 ID=MS.gene05446.t1.exon1;Parent=MS.gene05446.t1
chr6.4 CDS 58084885 58085214 58084885 ID=cds.MS.gene05446.t1;Parent=MS.gene05446.t1
chr6.4 exon 58085321 58085440 58085321 ID=MS.gene05446.t1.exon2;Parent=MS.gene05446.t1
chr6.4 CDS 58085321 58085440 58085321 ID=cds.MS.gene05446.t1;Parent=MS.gene05446.t1
chr6.4 exon 58085829 58085894 58085829 ID=MS.gene05446.t1.exon3;Parent=MS.gene05446.t1
chr6.4 CDS 58085829 58085894 58085829 ID=cds.MS.gene05446.t1;Parent=MS.gene05446.t1
chr6.4 exon 58086000 58086068 58086000 ID=MS.gene05446.t1.exon4;Parent=MS.gene05446.t1
chr6.4 CDS 58086000 58086068 58086000 ID=cds.MS.gene05446.t1;Parent=MS.gene05446.t1
chr6.4 exon 58086206 58086283 58086206 ID=MS.gene05446.t1.exon5;Parent=MS.gene05446.t1
chr6.4 CDS 58086206 58086283 58086206 ID=cds.MS.gene05446.t1;Parent=MS.gene05446.t1
chr6.4 exon 58086792 58086884 58086792 ID=MS.gene05446.t1.exon6;Parent=MS.gene05446.t1
chr6.4 CDS 58086792 58086884 58086792 ID=cds.MS.gene05446.t1;Parent=MS.gene05446.t1
Gene Sequence

>MS.gene05446

ATGGAACTATTGAATCAATTCATGGAGAATTCAAACATGAACATGATGCATAATTTGATGCCATTTTCTTGTGACAACATCTTGGAACATCATCAACAACAAGAACAAGAACATGTGTTTCCAAGAAACTTGGAAGAAAATTTTCATGGTCTTGTCCATCATGTCAATAATCAAAATCACAATGCAGTTCAACTTTCACTTCCAACAATTTTCCAAGAAGAAAATAAAGTTCATCATGAAGGTAAAAAGAGAAAAATGATGGATTTTCAAGAGACTAGTTCTGGAAATTCAACTCCTGCAGTTTCTGAAAGTGGAAGCAAAACCAAACTTGTAAATTTCTTCTACTTATTTTTTTTCATTTTATTCTTCATTTTTTGTGATTATGATGAAATTAAATTATTAACTGGTTTTGTTATTTTTCTATGATTAATTTCAGAGTGGTGGAAGAGGAAAAAGAGTGAAAAGCTATGAAACAGAAGAAGAGAAAGCAAAGGAAGTTGTTCATGTTAGAGCAAGAAGAGGCCAAGCTACTGATAGTCACAGTCTAGCAGAAAGGGTATAATTTCTTAAATACAGCTTAATTAAGTGTTTATAGCATAATCATTTATTATATAAGTGTCTATGCATAAACTATTTCTATAATAAATGGTAAAATCAAATCAAATTGGTTTTATATAAGGTATAAGTTGTTTTCGTAAGCTACACTGGTGAGCTTATATTAAAATAAATTAAAAACAATTTATGAATATGAGATAAGTTCATAAATTCTTCAAAATAGTCTTGCACATGTTTATATAGTAGTAGATAAGTTCAAATAAGTCATTACGAAAAGACAATAATCATTATGAATAGATTTAATGTCTTCATCAATTTTTTTTCTATCTTTGTTCATGATTGATGTGTTTGGACTAATAAATATCTAAAATATTTGTTTTGTTGTTCAGGTTAGAAGAGGGAAAATCAATGAAAAATTGAAGTGCTTGCAAAATATTGTTCCAGGATGTTATAAGGTTAATATATGTTCCTTTTTAATTTAAAAAAATATATAAACTCACAATTGTGTTTAATTTCATAATTTTTAAAATCTTATTTTGTTTTTGTTTCAATGGTTGCAGACCATGGGAATGGCAGTAATGTTGGATGAAATCATAAACTATGTTCAGTCTTTGCAGCATCAAGTAGAAGTAAGTAATATTCATTTATTTCTAGGAAAATTTAGTTTTAAAATATATTTAATTAATGTTAATTTATCACCTCAAAAAAATTATAATTAGTGTCAAAATAAATATTAATGTCAATTTATGTTTGGCTATTTTTGCAGTTTCTTTCTTTGAAGCTTACTGCAGCAAGTACTTATTATGATTTCAATTCAGAGACAGATGATCTAGAAACAATGCAGGTATAATCTATTTCTAAAAGTTTCATGGTTTTATTCGTATATAATATGCTCATATTCATGGTTTCTTTCATTTTGCATGGTTGTTAGGGTGCATGAAAACCTCTCAATTCATATAATAATATACGCTAACTGTTGTGATGGATAAGTTAAAATTCATTAGAGTTGGTGAATATGAAATTAAGTAGTTGATAGTTGATAAAATTAATTAGAAACAATTATTATATTTAGTAATATTTATAGGTTAATTTTTTTTAAAAGAAAGGTTAAATATGTTTTTAGTTTCTATAAGAATCATAAATTTTTAAGTTTAGTTCCAACTACATAAACTTTGTCCAATTTTAGCACCAATTTTGGTTCTTTAGGACTAATATTGAGACTAAATAACTCCTTTTAAAAAAAAGTTTTTTAATTAAATACTAAAATGAGACAAAATATATGTAAAATACTCTTCTTTTATTTATTTATTTATTTATTTATTTTATTTATTTATTTATTTGATTTGTGGCAGAGAGCAAGGGCATCTGAGGCAAAAGAGTTAGCAAGGTATAAGAGAGAAGGGTATGGAGGAATTGCTTGCTTTCAACCAACTTGGCCACTTTGA
Protein sequence

>MS.gene05446.t1

MELLNQFMENSNMNMMHNLMPFSCDNILEHHQQQEQEHVFPRNLEENFHGLVHHVNNQNHNAVQLSLPTIFQEENKVHHEGKKRKMMDFQETSSGNSTPAVSESGSKTKLSGGRGKRVKSYETEEEKAKEVVHVRARRGQATDSHSLAERVRRGKINEKLKCLQNIVPGCYKTMGMAVMLDEIINYVQSLQHQVEFLSLKLTAASTYYDFNSETDDLETMQRARASEAKELARYKREGYGGIACFQPTWPL