AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054540.t1 MTR_4g007490 95.526 380 10 1 1 380 1 373 0.0 737
MS.gene054540.t1 MTR_3g450500 67.717 381 116 5 1 380 1 375 1.54e-178 501
MS.gene054540.t1 MTR_2g019840 37.714 350 204 6 32 378 34 372 1.82e-69 223
MS.gene054540.t1 MTR_2g019810 37.158 366 218 5 14 378 6 360 1.80e-68 220
MS.gene054540.t1 MTR_4g119550 34.835 333 209 5 45 375 52 378 1.30e-58 195
MS.gene054540.t1 MTR_4g120380 32.194 351 224 5 27 374 30 369 4.76e-58 193
MS.gene054540.t1 MTR_4g120380 32.530 332 210 5 27 355 30 350 5.22e-55 187
MS.gene054540.t1 MTR_2g437260 29.851 335 226 3 43 374 35 363 2.59e-53 181
MS.gene054540.t1 MTR_2g437030 33.148 359 226 7 22 375 105 454 5.10e-53 182
MS.gene054540.t1 MTR_4g119580 32.877 365 225 6 22 379 23 374 8.62e-53 179
MS.gene054540.t1 MTR_2g437040 33.141 347 223 5 32 375 28 368 3.61e-52 178
MS.gene054540.t1 MTR_2g436460 33.526 346 210 6 36 374 41 373 4.37e-52 178
MS.gene054540.t1 MTR_2g436400 30.939 362 240 6 17 375 21 375 8.38e-50 172
MS.gene054540.t1 MTR_2g437130 31.746 378 244 8 1 375 3 369 1.47e-49 171
MS.gene054540.t1 MTR_2g437080 30.811 370 245 6 11 375 5 368 8.68e-49 169
MS.gene054540.t1 MTR_2g437160 31.339 351 229 8 29 375 51 393 5.14e-48 168
MS.gene054540.t1 MTR_2g437020 32.861 353 217 9 13 356 9 350 8.68e-48 167
MS.gene054540.t1 MTR_2g437150 31.405 363 237 7 22 375 29 388 8.83e-48 167
MS.gene054540.t1 MTR_2g436440 30.423 355 235 6 24 375 29 374 9.36e-47 164
MS.gene054540.t1 MTR_2g437060 31.282 390 231 9 10 375 7 383 1.43e-46 164
MS.gene054540.t1 MTR_2g437240 29.067 375 223 5 39 375 27 396 6.54e-46 162
MS.gene054540.t1 MTR_2g437960 29.970 337 228 6 46 378 55 387 6.23e-45 160
MS.gene054540.t1 MTR_2g437960 29.970 337 228 6 46 378 55 387 9.49e-45 160
MS.gene054540.t1 MTR_2g437120 29.805 359 241 6 22 375 29 381 2.18e-44 159
MS.gene054540.t1 MTR_2g437170 30.028 363 242 7 22 375 29 388 6.39e-44 157
MS.gene054540.t1 MTR_2g437100 29.288 379 252 8 15 380 23 398 7.24e-44 157
MS.gene054540.t1 MTR_2g437180 30.422 332 223 5 46 374 51 377 3.77e-43 155
MS.gene054540.t1 MTR_2g437200 28.947 380 225 8 35 375 23 396 4.14e-43 154
MS.gene054540.t1 MTR_2g076320 30.707 368 243 8 15 374 17 380 5.87e-42 152
MS.gene054540.t1 MTR_2g438010 29.620 368 247 8 15 374 37 400 6.66e-42 152
MS.gene054540.t1 MTR_2g437990 29.973 367 246 6 15 374 17 379 8.33e-42 152
MS.gene054540.t1 MTR_2g438020 27.596 366 226 3 46 375 73 435 3.86e-39 146
MS.gene054540.t1 MTR_8g081000 24.927 341 236 3 36 375 64 385 8.22e-31 121
MS.gene054540.t1 MTR_8g081000 24.927 341 236 3 36 375 64 385 8.96e-31 121
MS.gene054540.t1 MTR_8g081000 24.927 341 236 3 36 375 64 385 9.78e-31 121
MS.gene054540.t1 MTR_8g080990 27.485 342 238 5 44 380 41 377 3.05e-29 117
MS.gene054540.t1 MTR_2g437940 26.866 335 207 7 46 375 51 352 1.31e-28 115
MS.gene054540.t1 MTR_4g119570 28.205 234 129 3 138 371 31 225 1.06e-23 98.6
MS.gene054540.t1 MTR_2g437090 29.605 152 107 0 224 375 5 156 6.01e-21 89.4
MS.gene054540.t1 MTR_2g436370 37.143 175 103 5 27 198 30 200 8.36e-21 90.1
MS.gene054540.t1 MTR_2g437160 28.767 219 135 6 177 375 85 302 1.59e-20 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054540.t1 AT5G07900 41.176 340 197 3 37 374 61 399 2.58e-88 273
MS.gene054540.t1 AT1G21150 33.693 371 239 4 10 374 17 386 2.61e-61 202
MS.gene054540.t1 AT1G21150 33.693 371 239 4 10 374 81 450 1.17e-60 202
MS.gene054540.t1 AT1G21150 34.000 350 222 4 33 374 2 350 2.38e-59 196
MS.gene054540.t1 AT1G62120 30.179 391 226 7 14 360 18 405 3.68e-43 155
MS.gene054540.t1 AT1G61980 29.412 357 210 5 41 360 48 399 9.35e-40 146
MS.gene054540.t1 AT1G61980 29.412 357 210 5 41 360 48 399 9.35e-40 146
MS.gene054540.t1 AT1G61980 29.412 357 210 5 41 360 48 399 9.35e-40 146
MS.gene054540.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene054540.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene054540.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene054540.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene054540.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene054540.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene054540.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene054540.t1 AT1G61990 28.052 385 226 6 27 371 32 405 1.27e-39 145
MS.gene054540.t1 AT1G61990 28.052 385 226 6 27 371 32 405 1.27e-39 145
MS.gene054540.t1 AT3G46950 32.226 301 188 6 14 309 18 307 8.86e-38 141
MS.gene054540.t1 AT5G23930 29.120 443 222 10 7 370 12 441 5.40e-36 137
MS.gene054540.t1 AT1G62010 29.282 362 192 9 42 361 45 384 6.05e-36 135
MS.gene054540.t1 AT1G62150 31.900 279 175 5 35 307 48 317 1.29e-33 130
MS.gene054540.t1 AT1G62085 32.248 307 185 8 14 307 18 314 1.74e-32 127
MS.gene054540.t1 AT1G62085 32.248 307 185 8 14 307 18 314 1.74e-32 127
MS.gene054540.t1 AT1G61960 33.577 274 173 6 27 296 32 300 1.44e-31 124
MS.gene054540.t1 AT1G62110 31.633 294 174 7 27 307 32 311 3.62e-31 123
MS.gene054540.t1 AT5G64950 29.221 308 207 5 77 375 71 376 1.14e-30 121
MS.gene054540.t1 AT1G56380 23.161 367 231 11 39 361 10 369 2.12e-18 86.3
MS.gene054540.t1 AT1G56380 23.161 367 231 11 39 361 21 380 2.69e-18 86.3
MS.gene054540.t1 AT1G62490 27.341 267 150 7 30 296 83 305 2.64e-15 76.6

Find 66 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACCTTGTTGAAAGTTTA+AGG 0.085452 4.1:+87866227 None:intergenic
GAAAGATTTGCCATTGTTAT+TGG 0.156741 4.1:-87865321 MS.gene054540:CDS
CTTGTCACCAATCAAGATTT+TGG 0.169173 4.1:+87866021 None:intergenic
TAAGTATGGACAAAGAAATT+TGG 0.197332 4.1:-87865673 MS.gene054540:CDS
TATGAAAACAATGGACTTCT+TGG 0.234883 4.1:-87865546 MS.gene054540:CDS
GAATGCTACACAAGGAAAAT+TGG 0.258902 4.1:+87865793 None:intergenic
CCTAGTTTGCGATGATAAAA+AGG 0.296431 4.1:-87865942 MS.gene054540:CDS
TTCCTCACTTGTCATAATAA+AGG 0.304109 4.1:+87865376 None:intergenic
AAAGAACAGTAGTTGAATAA+AGG 0.305931 4.1:+87866368 None:intergenic
AATGGCAAATCTTTCTGAAA+TGG 0.311445 4.1:+87865329 None:intergenic
AAAAGCTGAATAACAGCATT+TGG 0.312391 4.1:+87866184 None:intergenic
ATAACAGCATTTGGGCCATT+AGG 0.321633 4.1:+87866193 None:intergenic
ACAGCAGAGAAAAGTGAAAA+AGG 0.329234 4.1:+87866331 None:intergenic
AAGTATGGACAAAGAAATTT+GGG 0.343578 4.1:-87865672 MS.gene054540:CDS
GATTTCCCCAAAATCTTGAT+TGG 0.348520 4.1:-87866028 MS.gene054540:CDS
TCAAAGTTCTATTTCCAGTT+TGG 0.350471 4.1:-87865819 MS.gene054540:CDS
TAACAGCATTTGGGCCATTA+GGG 0.351944 4.1:+87866194 None:intergenic
GGGCATGTAGCAATGTCTTC+TGG 0.354285 4.1:+87865503 None:intergenic
GTTAATTCAGTAAAAGAAAT+GGG 0.366519 4.1:-87865743 MS.gene054540:CDS
TCACATACTCAGGATACTTT+AGG 0.366731 4.1:+87865588 None:intergenic
ATGGCAAATCTTTCTGAAAT+GGG 0.369413 4.1:+87865330 None:intergenic
CAACCTTGTTGAAAGTTTAA+GGG 0.375048 4.1:+87866228 None:intergenic
ATCTTGATAACAGCACATTT+AGG 0.385800 4.1:+87865443 None:intergenic
AGGAAAATTGGATACCAAAC+TGG 0.392789 4.1:+87865805 None:intergenic
TGTTAATTCAGTAAAAGAAA+TGG 0.415470 4.1:-87865744 MS.gene054540:CDS
ACAACCCACATGAGTTTATG+AGG 0.423242 4.1:+87866263 None:intergenic
AAGTGTCTTCTCCAAGTTGC+GGG 0.424163 4.1:+87865469 None:intergenic
AAGAACAGTAGTTGAATAAA+GGG 0.427774 4.1:+87866369 None:intergenic
TCCAACACAAATCAACATAA+AGG 0.430035 4.1:-87866307 MS.gene054540:CDS
TAGCTTTGAGGCGTGGATCA+TGG 0.435733 4.1:-87865913 MS.gene054540:CDS
GATTCGAGTAATTGTAAGTA+TGG 0.436929 4.1:-87865687 MS.gene054540:CDS
TTCCTCAAAACCTCAATGTT+CGG 0.445259 4.1:+87865854 None:intergenic
AAAAGGTAGTTCTAGCTTTG+AGG 0.445792 4.1:-87865925 MS.gene054540:CDS
CTCAACAAATCTAGAATGCT+TGG 0.457146 4.1:+87865769 None:intergenic
ACCTTTATGTTGATTTGTGT+TGG 0.460913 4.1:+87866306 None:intergenic
AAGTTGCGGGTCAGAATCCC+AGG 0.461038 4.1:+87865482 None:intergenic
AAAGCTGAATAACAGCATTT+GGG 0.472696 4.1:+87866185 None:intergenic
TCAAGAGGTTTAATAAAGAG+TGG 0.472917 4.1:-87865413 MS.gene054540:CDS
GAAGACATTGCTACATGCCC+TGG 0.473610 4.1:-87865500 MS.gene054540:CDS
CAAAAGGGTGTTTCTTAACA+AGG 0.481935 4.1:+87866125 None:intergenic
TTGCAACTCATAAACCCTAA+TGG 0.484587 4.1:-87866208 MS.gene054540:CDS
GGACTTCTTGGTGAAGGATG+TGG 0.492155 4.1:-87865534 MS.gene054540:CDS
TATGAGGTTTGACAATGTAA+AGG 0.499828 4.1:+87866279 None:intergenic
TGGGATTCTGACCCGCAACT+TGG 0.503137 4.1:-87865480 MS.gene054540:CDS
ATCAATGGTTGAAGCCCCAA+TGG 0.538102 4.1:+87866048 None:intergenic
CAAGCATTCTAGATTTGTTG+AGG 0.552143 4.1:-87865768 MS.gene054540:CDS
GACTTCTTGGTGAAGGATGT+GGG 0.555487 4.1:-87865533 MS.gene054540:CDS
GCAATGAGAAAATGATACAT+TGG 0.568531 4.1:+87866415 None:intergenic
AAAACAAAATACAACTTCGA+AGG 0.568698 4.1:+87865713 None:intergenic
TCTGAGATCATCACATACTC+AGG 0.569253 4.1:+87865578 None:intergenic
TGATTCTGTTCCGAACATTG+AGG 0.570760 4.1:-87865864 MS.gene054540:CDS
TCAAACCTCATAAACTCATG+TGG 0.578097 4.1:-87866268 MS.gene054540:CDS
GTAGTTCTAGCTTTGAGGCG+TGG 0.585169 4.1:-87865920 MS.gene054540:CDS
AACAATGGACTTCTTGGTGA+AGG 0.587900 4.1:-87865540 MS.gene054540:CDS
AAGACATTGCTACATGCCCT+GGG 0.601471 4.1:-87865499 MS.gene054540:CDS
CAAGTGTCTTCTCCAAGTTG+CGG 0.602943 4.1:+87865468 None:intergenic
AAACTGGAAATAGAACTTTG+AGG 0.609479 4.1:+87865821 None:intergenic
TACCTTTATTATGACAAGTG+AGG 0.612836 4.1:-87865378 MS.gene054540:CDS
TGGCAAATCTTTCTGAAATG+GGG 0.627523 4.1:+87865331 None:intergenic
TTATATGCATCCAATAACAA+TGG 0.629215 4.1:+87865311 None:intergenic
TTAATTCAGTAAAAGAAATG+GGG 0.635626 4.1:-87865742 MS.gene054540:CDS
CGGAACAGAATCATTAACCA+TGG 0.638798 4.1:+87865874 None:intergenic
CAAACCTCATAAACTCATGT+GGG 0.639091 4.1:-87866267 MS.gene054540:CDS
AAGCCCTTAAACTTTCAACA+AGG 0.642886 4.1:-87866231 MS.gene054540:CDS
TGCTTGGTGAATGCTACACA+AGG 0.683625 4.1:+87865785 None:intergenic
AGTTGCGGGTCAGAATCCCA+GGG 0.702455 4.1:+87865483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAATTATGAAAACAA+TGG - chr4.1:87866149-87866168 MS.gene054540:CDS 15.0%
!!! ATTTTGATAAAAGTACAAAA+GGG + chr4.1:87865597-87865616 None:intergenic 15.0%
!! GTTAATTCAGTAAAAGAAAT+GGG - chr4.1:87865961-87865980 MS.gene054540:CDS 20.0%
!! TGTTAATTCAGTAAAAGAAA+TGG - chr4.1:87865960-87865979 MS.gene054540:CDS 20.0%
!! TTAATTCAGTAAAAGAAATG+GGG - chr4.1:87865962-87865981 MS.gene054540:CDS 20.0%
!!! GATTTTGATAAAAGTACAAA+AGG + chr4.1:87865598-87865617 None:intergenic 20.0%
! AAAACAAAATACAACTTCGA+AGG + chr4.1:87865994-87866013 None:intergenic 25.0%
! AAAGAACAGTAGTTGAATAA+AGG + chr4.1:87865339-87865358 None:intergenic 25.0%
! AAGAACAGTAGTTGAATAAA+GGG + chr4.1:87865338-87865357 None:intergenic 25.0%
! AAGTATGGACAAAGAAATTT+GGG - chr4.1:87866032-87866051 MS.gene054540:CDS 25.0%
! TAAGTATGGACAAAGAAATT+TGG - chr4.1:87866031-87866050 MS.gene054540:CDS 25.0%
! TTATATGCATCCAATAACAA+TGG + chr4.1:87866396-87866415 None:intergenic 25.0%
!!! CAAAATTTAAGATTTTCGAG+AGG - chr4.1:87866058-87866077 MS.gene054540:CDS 25.0%
!!! TTCGAAGTTGTATTTTGTTT+TGG - chr4.1:87865993-87866012 MS.gene054540:CDS 25.0%
AAAAGCTGAATAACAGCATT+TGG + chr4.1:87865523-87865542 None:intergenic 30.0%
AAACTGGAAATAGAACTTTG+AGG + chr4.1:87865886-87865905 None:intergenic 30.0%
AAAGCTGAATAACAGCATTT+GGG + chr4.1:87865522-87865541 None:intergenic 30.0%
CAACCTTGTTGAAAGTTTAA+GGG + chr4.1:87865479-87865498 None:intergenic 30.0%
GAAAGATTTGCCATTGTTAT+TGG - chr4.1:87866383-87866402 MS.gene054540:CDS 30.0%
GATTCGAGTAATTGTAAGTA+TGG - chr4.1:87866017-87866036 MS.gene054540:CDS 30.0%
TACCTTTATTATGACAAGTG+AGG - chr4.1:87866326-87866345 MS.gene054540:CDS 30.0%
TATGAGGTTTGACAATGTAA+AGG + chr4.1:87865428-87865447 None:intergenic 30.0%
TCAAAGTTCTATTTCCAGTT+TGG - chr4.1:87865885-87865904 MS.gene054540:CDS 30.0%
TCAAGAGGTTTAATAAAGAG+TGG - chr4.1:87866291-87866310 MS.gene054540:CDS 30.0%
TCCAACACAAATCAACATAA+AGG - chr4.1:87865397-87865416 MS.gene054540:CDS 30.0%
TTCCTCACTTGTCATAATAA+AGG + chr4.1:87866331-87866350 None:intergenic 30.0%
! AATGGCAAATCTTTCTGAAA+TGG + chr4.1:87866378-87866397 None:intergenic 30.0%
! ATCTTGATAACAGCACATTT+AGG + chr4.1:87866264-87866283 None:intergenic 30.0%
! GAACTTTTACAATGATTCCA+TGG - chr4.1:87865813-87865832 MS.gene054540:CDS 30.0%
! GATTGCAAAAACTTGAGTTT+TGG + chr4.1:87865637-87865656 None:intergenic 30.0%
! TATGAAAACAATGGACTTCT+TGG - chr4.1:87866158-87866177 MS.gene054540:CDS 30.0%
! TCAAGTTTTTGCAATCCATT+GGG - chr4.1:87865641-87865660 MS.gene054540:CDS 30.0%
! TTGTCACCAATCAAGATTTT+GGG + chr4.1:87865685-87865704 None:intergenic 30.0%
!! AAAACTTGAGTTTTGGCAAA+AGG + chr4.1:87865630-87865649 None:intergenic 30.0%
!! AAACTTGAGTTTTGGCAAAA+GGG + chr4.1:87865629-87865648 None:intergenic 30.0%
!! AATTTAAGATTTTCGAGAGG+TGG - chr4.1:87866061-87866080 MS.gene054540:CDS 30.0%
!! ACCTTTATGTTGATTTGTGT+TGG + chr4.1:87865401-87865420 None:intergenic 30.0%
!! ATGGCAAATCTTTCTGAAAT+GGG + chr4.1:87866377-87866396 None:intergenic 30.0%
!! ATTTAAGATTTTCGAGAGGT+GGG - chr4.1:87866062-87866081 MS.gene054540:CDS 30.0%
!! CTTTTAAGAATCTTGTAACG+AGG + chr4.1:87865742-87865761 None:intergenic 30.0%
!!! ATCAAGATTTTGAAGTCAAG+AGG - chr4.1:87866276-87866295 MS.gene054540:CDS 30.0%
AAGCCCTTAAACTTTCAACA+AGG - chr4.1:87865473-87865492 MS.gene054540:CDS 35.0%
ACAGCAGAGAAAAGTGAAAA+AGG + chr4.1:87865376-87865395 None:intergenic 35.0%
AGGAAAATTGGATACCAAAC+TGG + chr4.1:87865902-87865921 None:intergenic 35.0%
CAAACCTCATAAACTCATGT+GGG - chr4.1:87865437-87865456 MS.gene054540:CDS 35.0%
GAATGCTACACAAGGAAAAT+TGG + chr4.1:87865914-87865933 None:intergenic 35.0%
GATTTCCCCAAAATCTTGAT+TGG - chr4.1:87865676-87865695 MS.gene054540:CDS 35.0%
GCAACCTTGTTGAAAGTTTA+AGG + chr4.1:87865480-87865499 None:intergenic 35.0%
TCAAACCTCATAAACTCATG+TGG - chr4.1:87865436-87865455 MS.gene054540:CDS 35.0%
TCACATACTCAGGATACTTT+AGG + chr4.1:87866119-87866138 None:intergenic 35.0%
TTCCTCAAAACCTCAATGTT+CGG + chr4.1:87865853-87865872 None:intergenic 35.0%
TTGCAACTCATAAACCCTAA+TGG - chr4.1:87865496-87865515 MS.gene054540:CDS 35.0%
! ATTCAGCTTTTCAGAAGCTA+TGG - chr4.1:87865532-87865551 MS.gene054540:CDS 35.0%
! CAAGTTTTTGCAATCCATTG+GGG - chr4.1:87865642-87865661 MS.gene054540:CDS 35.0%
! CCTAGTTTGCGATGATAAAA+AGG - chr4.1:87865762-87865781 MS.gene054540:CDS 35.0%
! CCTTTTTATCATCGCAAACT+AGG + chr4.1:87865765-87865784 None:intergenic 35.0%
! CTCAACAAATCTAGAATGCT+TGG + chr4.1:87865938-87865957 None:intergenic 35.0%
! CTCAAGTTTTTGCAATCCAT+TGG - chr4.1:87865640-87865659 MS.gene054540:CDS 35.0%
! CTTGTCACCAATCAAGATTT+TGG + chr4.1:87865686-87865705 None:intergenic 35.0%
! TGTCACCAATCAAGATTTTG+GGG + chr4.1:87865684-87865703 None:intergenic 35.0%
!! AAAAGGTAGTTCTAGCTTTG+AGG - chr4.1:87865779-87865798 MS.gene054540:CDS 35.0%
!! CAAAAGGGTGTTTCTTAACA+AGG + chr4.1:87865582-87865601 None:intergenic 35.0%
!! CAAGATTTTGGGGAAATCAA+TGG + chr4.1:87865674-87865693 None:intergenic 35.0%
!! CAAGCATTCTAGATTTGTTG+AGG - chr4.1:87865936-87865955 MS.gene054540:CDS 35.0%
!! GAAAGTTTAAGGGCTTTTTC+AGG + chr4.1:87865469-87865488 None:intergenic 35.0%
!! TGGCAAATCTTTCTGAAATG+GGG + chr4.1:87866376-87866395 None:intergenic 35.0%
!! TTTAAGATTTTCGAGAGGTG+GGG - chr4.1:87866063-87866082 MS.gene054540:CDS 35.0%
ACAACCCACATGAGTTTATG+AGG + chr4.1:87865444-87865463 None:intergenic 40.0%
ATAACAGCATTTGGGCCATT+AGG + chr4.1:87865514-87865533 None:intergenic 40.0%
CGGAACAGAATCATTAACCA+TGG + chr4.1:87865833-87865852 None:intergenic 40.0%
TAACAGCATTTGGGCCATTA+GGG + chr4.1:87865513-87865532 None:intergenic 40.0%
TGATTCTGTTCCGAACATTG+AGG - chr4.1:87865840-87865859 MS.gene054540:CDS 40.0%
! AACAATGGACTTCTTGGTGA+AGG - chr4.1:87866164-87866183 MS.gene054540:CDS 40.0%
! CAAGTTTTTGACAGCAAGCT+TGG - chr4.1:87865702-87865721 MS.gene054540:CDS 40.0%
! TCTGAGATCATCACATACTC+AGG + chr4.1:87866129-87866148 None:intergenic 40.0%
! TTCCGAACATTGAGGTTTTG+AGG - chr4.1:87865848-87865867 MS.gene054540:CDS 40.0%
!!! ATTGAGGTTTTGAGGAAGCT+TGG - chr4.1:87865856-87865875 MS.gene054540:CDS 40.0%
AAGACATTGCTACATGCCCT+GGG - chr4.1:87866205-87866224 MS.gene054540:CDS 45.0%
AAGTGTCTTCTCCAAGTTGC+GGG + chr4.1:87866238-87866257 None:intergenic 45.0%
ATCAATGGTTGAAGCCCCAA+TGG + chr4.1:87865659-87865678 None:intergenic 45.0%
CAAGTGTCTTCTCCAAGTTG+CGG + chr4.1:87866239-87866258 None:intergenic 45.0%
GACTTCTTGGTGAAGGATGT+GGG - chr4.1:87866171-87866190 MS.gene054540:CDS 45.0%
! TGCTTGGTGAATGCTACACA+AGG + chr4.1:87865922-87865941 None:intergenic 45.0%
GAAGACATTGCTACATGCCC+TGG - chr4.1:87866204-87866223 MS.gene054540:CDS 50.0%
GGACTTCTTGGTGAAGGATG+TGG - chr4.1:87866170-87866189 MS.gene054540:CDS 50.0%
GGGCATGTAGCAATGTCTTC+TGG + chr4.1:87866204-87866223 None:intergenic 50.0%
!! GTAGTTCTAGCTTTGAGGCG+TGG - chr4.1:87865784-87865803 MS.gene054540:CDS 50.0%
!! TAGCTTTGAGGCGTGGATCA+TGG - chr4.1:87865791-87865810 MS.gene054540:CDS 50.0%
AAGTTGCGGGTCAGAATCCC+AGG + chr4.1:87866225-87866244 None:intergenic 55.0%
AGTTGCGGGTCAGAATCCCA+GGG + chr4.1:87866224-87866243 None:intergenic 55.0%
TGGGATTCTGACCCGCAACT+TGG - chr4.1:87866224-87866243 MS.gene054540:CDS 55.0%
!! AGATTTTCGAGAGGTGGGGC+TGG - chr4.1:87866067-87866086 MS.gene054540:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 87865292 87866434 87865292 ID=MS.gene054540
chr4.1 mRNA 87865292 87866434 87865292 ID=MS.gene054540.t1;Parent=MS.gene054540
chr4.1 exon 87865292 87866434 87865292 ID=MS.gene054540.t1.exon1;Parent=MS.gene054540.t1
chr4.1 CDS 87865292 87866434 87865292 ID=cds.MS.gene054540.t1;Parent=MS.gene054540.t1
Gene Sequence

>MS.gene054540

ATGTATCATTTTCTCATTGCTAGATTCACTCATACTCATAGAACCCTTTATTCAACTACTGTTCTTTATCACAGACACAATCCTTTTTCACTTTTCTCTGCTGTTTCCAACACAAATCAACATAAAGGTGACACCTTTACATTGTCAAACCTCATAAACTCATGTGGGTTGTCTCCTGAAAAAGCCCTTAAACTTTCAACAAGGTTGCAACTCATAAACCCTAATGGCCCAAATGCTGTTATTCAGCTTTTCAGAAGCTATGGTTTCTCTGATTCTCAGTTATCAAGCCTTGTTAAGAAACACCCTTTTGTACTTTTATCAAAATCTGATAAGACCCTTTTGCCAAAACTCAAGTTTTTGCAATCCATTGGGGCTTCAACCATTGATTTCCCCAAAATCTTGATTGGTGACAAGTTTTTGACAGCAAGCTTGGAGAAAACGATAATACCTCGTTACAAGATTCTTAAAAGCCTAGTTTGCGATGATAAAAAGGTAGTTCTAGCTTTGAGGCGTGGATCATGGAACTTTTACAATGATTCCATGGTTAATGATTCTGTTCCGAACATTGAGGTTTTGAGGAAGCTTGGTGTGCCTCAAAGTTCTATTTCCAGTTTGGTATCCAATTTTCCTTGTGTAGCATTCACCAAGCATTCTAGATTTGTTGAGGCTGTTAATTCAGTAAAAGAAATGGGGTTCAATCCTTCGAAGTTGTATTTTGTTTTGGCGATTCGAGTAATTGTAAGTATGGACAAAGAAATTTGGGAATCAAAATTTAAGATTTTCGAGAGGTGGGGCTGGTCGAGAGATATTTGTCGTTTCGCTTTCCTAAAGTATCCTGAGTATGTGATGATCTCAGAGAAAAAAATTATGAAAACAATGGACTTCTTGGTGAAGGATGTGGGTCTTACACCAGAAGACATTGCTACATGCCCTGGGATTCTGACCCGCAACTTGGAGAAGACACTTGTACCTAAATGTGCTGTTATCAAGATTTTGAAGTCAAGAGGTTTAATAAAGAGTGGTTTGCGTGTAAGTACCTTTATTATGACAAGTGAGGAAAAGTTTGTGCAGAAATATGTGACCCCATTTCAGAAAGATTTGCCATTGTTATTGGATGCATATAAAGTTCAGAAATCAGATTGA
Protein sequence

>MS.gene054540.t1

MYHFLIARFTHTHRTLYSTTVLYHRHNPFSLFSAVSNTNQHKGDTFTLSNLINSCGLSPEKALKLSTRLQLINPNGPNAVIQLFRSYGFSDSQLSSLVKKHPFVLLSKSDKTLLPKLKFLQSIGASTIDFPKILIGDKFLTASLEKTIIPRYKILKSLVCDDKKVVLALRRGSWNFYNDSMVNDSVPNIEVLRKLGVPQSSISSLVSNFPCVAFTKHSRFVEAVNSVKEMGFNPSKLYFVLAIRVIVSMDKEIWESKFKIFERWGWSRDICRFAFLKYPEYVMISEKKIMKTMDFLVKDVGLTPEDIATCPGILTRNLEKTLVPKCAVIKILKSRGLIKSGLRVSTFIMTSEEKFVQKYVTPFQKDLPLLLDAYKVQKSD