AlfalfaGEDB | MS.gene054715

M. sativa cultivar XinJiangDaYe / MS.gene054715

KEGG: -
GO: -
PFAM: PF03101.18 PF10551.12

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054715.t1	XP_024642224.1	72.2	468	128	1	1	466	1	468	5.60E-202	713.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054715.t1	Q9SWG3	37.1	456	271	8	3	449	25	473	2.0e-83	311.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054715.t1	A0A2Z6M8U9	68.9	466	141	1	1	466	1	462	7.7e-193	682.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene054715.t1	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054715.t1	MTR_0856s0010	59.375	160	63	1	1	158	1	160	2.49e-58	190
MS.gene054715.t1	MTR_6g051425	32.342	269	168	6	42	300	27	291	1.06e-37	141
MS.gene054715.t1	MTR_7g115320	66.265	83	25	1	119	201	6	85	7.22e-28	106
MS.gene054715.t1	MTR_0558s0020	25.869	259	165	6	36	269	40	296	1.72e-20	92.0
MS.gene054715.t1	MTR_4g035820	29.368	269	158	11	25	273	68	324	4.41e-20	94.0
MS.gene054715.t1	MTR_1g008290	32.558	129	84	1	97	225	19	144	3.82e-19	84.3
MS.gene054715.t1	MTR_8g446020	28.177	181	124	3	93	269	78	256	7.81e-18	83.6
MS.gene054715.t1	MTR_1g070355	26.496	234	160	7	38	263	80	309	2.21e-17	85.5
MS.gene054715.t1	MTR_7g446480	31.285	179	121	2	105	281	140	318	5.03e-17	82.4
MS.gene054715.t1	MTR_7g446510	30.726	179	122	2	105	281	155	333	5.69e-17	82.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054715.t1	AT4G15090	36.383	459	280	7	3	454	25	478	7.81e-98	313
MS.gene054715.t1	AT4G15090	36.383	459	280	7	3	454	25	478	7.81e-98	313
MS.gene054715.t1	AT4G15090	36.383	459	280	7	3	454	25	478	7.81e-98	313
MS.gene054715.t1	AT4G15090	36.383	459	280	7	3	454	25	478	7.81e-98	313
MS.gene054715.t1	AT4G15090	36.383	459	280	7	3	454	25	478	7.81e-98	313
MS.gene054715.t1	AT4G38180	35.398	452	274	7	26	462	62	510	9.01e-93	299
MS.gene054715.t1	AT1G52520	34.571	431	267	7	32	459	85	503	2.75e-86	280
MS.gene054715.t1	AT1G76320	31.659	458	299	6	36	490	1	447	1.54e-79	261
MS.gene054715.t1	AT3G22170	32.473	465	271	6	26	471	64	504	3.40e-79	264
MS.gene054715.t1	AT3G22170	32.473	465	271	6	26	471	64	504	3.40e-79	264

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Find 76 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCATGTCATATTTAT+TGG	0.272458	5.3:-32850767	None:intergenic
AACAGAGAAAGGCGTATCAT+TGG	0.454227	5.3:+32850556	MS.gene054715:CDS
AACATAATGATTGGCTGAGT+GGG	0.556371	5.3:+32851157	MS.gene054715:CDS
AACTTAGCAGTAATCTTTGC+AGG	0.345757	5.3:-32850295	None:intergenic
AACTTTATTTGGATGTGGTC+TGG	0.372803	5.3:+32850825	MS.gene054715:CDS
AAGACACAGAGTCCTTTGTG+TGG	0.707585	5.3:+32850857	MS.gene054715:CDS
AAGAGAAGGACATCATGTGT+CGG	0.593763	5.3:+32850225	MS.gene054715:CDS
AAGATTAGAACACCAAAGAA+AGG	0.546564	5.3:+32850163	MS.gene054715:CDS
AATCCAAATCAATGATGAAG+CGG	0.605155	5.3:+32850438	MS.gene054715:CDS
AATGGAGTTGATAGCTTTAG+TGG	0.398440	5.3:+32850037	MS.gene054715:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATTAGAACACCAAAGAA+AGG	+	chr5.3:32850163-32850182	MS.gene054715:CDS	30.0%
AATCCAAATCAATGATGAAG+CGG	+	chr5.3:32850438-32850457	MS.gene054715:CDS	30.0%
AGTTGGGAAAAGATGAATTA+TGG	+	chr5.3:32850314-32850333	MS.gene054715:CDS	30.0%
CATCTGAATTCGAAGAAAAA+TGG	+	chr5.3:32851106-32851125	MS.gene054715:CDS	30.0%
CATGGTCAATCAACTTTATT+TGG	+	chr5.3:32850814-32850833	MS.gene054715:CDS	30.0%
GGAATTATGTTAACAGAGAA+AGG	+	chr5.3:32850545-32850564	MS.gene054715:CDS	30.0%
TATTATCACTGACCAATGTA+AGG	+	chr5.3:32850927-32850946	MS.gene054715:CDS	30.0%
TCACATGAGTATATTCTTCT+TGG	-	chr5.3:32851439-32851458	None:intergenic	30.0%
TTGAATAACCTTGACTTAGT+GGG	-	chr5.3:32850382-32850401	None:intergenic	30.0%
AACTTAGCAGTAATCTTTGC+AGG	-	chr5.3:32850298-32850317	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	32849986	32851458	32849986	ID=MS.gene054715
chr5.3	mRNA	32849986	32851458	32849986	ID=MS.gene054715.t1;Parent=MS.gene054715
chr5.3	exon	32849986	32851458	32849986	ID=MS.gene054715.t1.exon1;Parent=MS.gene054715.t1
chr5.3	CDS	32849986	32851458	32849986	ID=cds.MS.gene054715.t1;Parent=MS.gene054715.t1

Gene Sequence

>MS.gene054715

ATGGATAGTCCAAACAATTTTGATGTTTGTGATGGTTTTGATGGTGAAGCTAATGGAGTTGATAGCTTTAGTGGTGAACAAGCAAGTTGCGAGCCTTCATCAGGGATGTCCTTTTCATATGTAGATGAGGTGAAATCATTTTATAAAGAATATGCTTCTAAAAAGGGTTTTGATTGGAAGATTAGAACACCAAAGAAAGGAGAGGATGGGAATCTCAAGTACTTCATGCTATCTTGCTCAAGAGAAGGACATCATGTGTCGGGAGTACCATGTACATTGAAGACACTTCCGACTAAAAGGAAAAGTTGTCCTGCAAAGATTACTGCTAAGTTGGGAAAAGATGAATTATGGTATATTAAAAAGTTTGAACCTAACCACTCTCACACAATTAGCCCCACTAAGTCAAGGTTATTCAAGGCAAACAAAAATATGAACATGCATGCAAAGAGAACAATCCAAATCAATGATGAAGCGGGCGTGAGGATCAACAAGACTTTTCAGGCTATTGTTAGAGATGCTGGAGGTTATGAAAATGTAGCATTTTGTGAAAGAGATGTTAGGAATTATGTTAACAGAGAAAGGCGTATCATTGGAAAAGGAGGAGACGGCAAAGCATTGCTAAATTATTTTTGTCGAATGAGGGAGCAAAATACAAATTTCTTCTATGATATAGATTTGGAAGATGATTTTCACGTACGAAACGTGTTTTGGGCTGATGCAAGAAGCAGAGTTGCATATGAATATTTTGGGGATGTTGTGACTTTCGATACAACGTATTTGACCAATAAATATGACATGCCTTTTGCTGCATTTGTTGGTGTGAATCACCATGGTCAATCAACTTTATTTGGATGTGGTCTGGCTTCAGCTAAAGACACAGAGTCCTTTGTGTGGCTTTTTCAATCATGGCTTCGTTGTATGCAAGGGAAGGCTCCTGCAGGTATTATCACTGACCAATGTAAGGCTATGCAAAATGTTATTCAGTCAGTATTCCCTACTACTAGACATAGGTGGTGTTTGTGGCACATAATGGCCAAAATCCCTGAAAAGCTTAGTAGATATGGTAAATATAAGAATATAAAGTTTGCAATGAAAGAAGCAGTTTATGATTCACTTACACCATCTGAATTCGAAGAAAAATGGTGTTATTTTGTTAACAAATTTGAGCTGGAACATAATGATTGGCTGAGTGGGTTGTACAATGAGCGCCATAGATGGGTGCCAATTATGTTGAAAAAATCTTTTTGGGCTGGTATGTCGACAACACAACGGAGTGAGAGCATACATGCTTTCTTTGATGGATATATCAATTCGACAACCAGTTTGCATCAATTTGTAAATCAATATGATAATGCTCTCAAAGTAGAGCAGAAAAAGAGTTTGAAGCTGATTTTCAATCAATGGACACAACAATTCCTTGTGGGTCAAGCTCGTCGATCGAAAAGCAATTCCAAGAAGAATATACTCATGTGA

Protein sequence

>MS.gene054715.t1

MDSPNNFDVCDGFDGEANGVDSFSGEQASCEPSSGMSFSYVDEVKSFYKEYASKKGFDWKIRTPKKGEDGNLKYFMLSCSREGHHVSGVPCTLKTLPTKRKSCPAKITAKLGKDELWYIKKFEPNHSHTISPTKSRLFKANKNMNMHAKRTIQINDEAGVRINKTFQAIVRDAGGYENVAFCERDVRNYVNRERRIIGKGGDGKALLNYFCRMREQNTNFFYDIDLEDDFHVRNVFWADARSRVAYEYFGDVVTFDTTYLTNKYDMPFAAFVGVNHHGQSTLFGCGLASAKDTESFVWLFQSWLRCMQGKAPAGIITDQCKAMQNVIQSVFPTTRHRWCLWHIMAKIPEKLSRYGKYKNIKFAMKEAVYDSLTPSEFEEKWCYFVNKFELEHNDWLSGLYNERHRWVPIMLKKSFWAGMSTTQRSESIHAFFDGYINSTTSLHQFVNQYDNALKVEQKKSLKLIFNQWTQQFLVGQARRSKSNSKKNILM