Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054790.t1 | XP_003614286.1 | 96.2 | 156 | 6 | 0 | 1 | 156 | 1 | 156 | 8.30E-75 | 289.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054790.t1 | Q9FJK3 | 47.4 | 156 | 81 | 1 | 1 | 155 | 1 | 156 | 2.2e-31 | 136.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054790.t1 | G7JY85 | 96.2 | 156 | 6 | 0 | 1 | 156 | 1 | 156 | 6.0e-75 | 289.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene054790.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054790.t1 | MTR_5g047580 | 96.154 | 156 | 6 | 0 | 1 | 156 | 1 | 156 | 1.03e-108 | 306 |
| MS.gene054790.t1 | MTR_1g084950 | 85.897 | 156 | 22 | 0 | 1 | 156 | 1 | 156 | 5.77e-88 | 254 |
| MS.gene054790.t1 | MTR_5g047560 | 82.051 | 156 | 12 | 1 | 1 | 156 | 1 | 140 | 2.61e-87 | 251 |
| MS.gene054790.t1 | MTR_7g011950 | 75.000 | 156 | 39 | 0 | 1 | 156 | 1 | 156 | 5.43e-76 | 223 |
| MS.gene054790.t1 | MTR_4g028720 | 75.000 | 156 | 39 | 0 | 1 | 156 | 1 | 156 | 2.09e-75 | 222 |
| MS.gene054790.t1 | MTR_1g077390 | 68.590 | 156 | 49 | 0 | 1 | 156 | 1 | 156 | 7.13e-75 | 221 |
| MS.gene054790.t1 | MTR_1g077320 | 72.436 | 156 | 43 | 0 | 1 | 156 | 1 | 156 | 1.52e-73 | 217 |
| MS.gene054790.t1 | MTR_1g077300 | 71.154 | 156 | 43 | 1 | 1 | 156 | 1 | 154 | 1.03e-67 | 202 |
| MS.gene054790.t1 | MTR_1g090783 | 64.706 | 153 | 54 | 0 | 3 | 155 | 2 | 154 | 6.05e-66 | 198 |
| MS.gene054790.t1 | MTR_1g090697 | 59.355 | 155 | 63 | 0 | 1 | 155 | 1 | 155 | 7.33e-62 | 187 |
| MS.gene054790.t1 | MTR_1g090710 | 57.051 | 156 | 66 | 1 | 1 | 155 | 1 | 156 | 3.11e-59 | 181 |
| MS.gene054790.t1 | MTR_3g065100 | 51.282 | 156 | 75 | 1 | 1 | 155 | 1 | 156 | 9.18e-43 | 142 |
| MS.gene054790.t1 | MTR_3g466980 | 44.805 | 154 | 84 | 1 | 1 | 153 | 1 | 154 | 3.61e-40 | 135 |
| MS.gene054790.t1 | MTR_4g032620 | 40.909 | 154 | 89 | 2 | 1 | 152 | 1 | 154 | 1.33e-39 | 134 |
| MS.gene054790.t1 | MTR_4g063790 | 42.857 | 154 | 86 | 2 | 1 | 152 | 1 | 154 | 2.10e-36 | 124 |
| MS.gene054790.t1 | MTR_3g466830 | 42.857 | 154 | 87 | 1 | 3 | 155 | 2 | 155 | 7.25e-34 | 117 |
| MS.gene054790.t1 | MTR_8g036130 | 42.767 | 159 | 87 | 2 | 1 | 155 | 1 | 159 | 8.29e-34 | 119 |
| MS.gene054790.t1 | MTR_5g075380 | 35.897 | 156 | 99 | 1 | 1 | 155 | 1 | 156 | 9.34e-33 | 114 |
| MS.gene054790.t1 | MTR_4g031910 | 43.590 | 156 | 86 | 2 | 1 | 154 | 1 | 156 | 1.34e-32 | 115 |
| MS.gene054790.t1 | MTR_3g467080 | 38.217 | 157 | 95 | 2 | 1 | 155 | 1 | 157 | 9.72e-31 | 108 |
| MS.gene054790.t1 | MTR_3g031240 | 37.580 | 157 | 96 | 2 | 1 | 155 | 1 | 157 | 1.10e-30 | 109 |
| MS.gene054790.t1 | MTR_3g466890 | 39.610 | 154 | 92 | 1 | 3 | 155 | 2 | 155 | 1.09e-29 | 106 |
| MS.gene054790.t1 | MTR_4g032260 | 42.949 | 156 | 87 | 2 | 1 | 154 | 1 | 156 | 5.03e-29 | 104 |
| MS.gene054790.t1 | MTR_3g031100 | 37.342 | 158 | 95 | 3 | 1 | 155 | 1 | 157 | 6.41e-29 | 106 |
| MS.gene054790.t1 | MTR_2g035610 | 45.763 | 118 | 63 | 1 | 1 | 117 | 1 | 118 | 1.14e-28 | 102 |
| MS.gene054790.t1 | MTR_4g032290 | 44.231 | 156 | 85 | 2 | 1 | 154 | 1 | 156 | 2.03e-28 | 105 |
| MS.gene054790.t1 | MTR_2g016210 | 37.748 | 151 | 92 | 2 | 1 | 149 | 1 | 151 | 8.05e-27 | 101 |
| MS.gene054790.t1 | MTR_4g028800 | 53.425 | 73 | 32 | 1 | 1 | 73 | 1 | 71 | 2.91e-23 | 87.4 |
| MS.gene054790.t1 | MTR_3g465410 | 38.889 | 126 | 75 | 2 | 29 | 152 | 1 | 126 | 2.96e-23 | 89.4 |
| MS.gene054790.t1 | MTR_2g035580 | 34.194 | 155 | 101 | 1 | 1 | 154 | 1 | 155 | 3.11e-23 | 89.7 |
| MS.gene054790.t1 | MTR_4g019670 | 34.899 | 149 | 85 | 4 | 1 | 145 | 1 | 141 | 1.28e-18 | 82.0 |
| MS.gene054790.t1 | MTR_7g106510 | 32.639 | 144 | 88 | 3 | 3 | 145 | 6 | 141 | 1.00e-17 | 79.3 |
| MS.gene054790.t1 | MTR_1g033930 | 46.237 | 93 | 49 | 1 | 64 | 155 | 22 | 114 | 2.38e-17 | 73.6 |
| MS.gene054790.t1 | MTR_3g466900 | 34.400 | 125 | 81 | 1 | 32 | 155 | 2 | 126 | 2.81e-17 | 73.6 |
| MS.gene054790.t1 | MTR_3g466930 | 34.400 | 125 | 81 | 1 | 32 | 155 | 2 | 126 | 3.96e-17 | 73.2 |
| MS.gene054790.t1 | MTR_7g055800 | 35.043 | 117 | 49 | 2 | 1 | 117 | 1 | 90 | 2.23e-16 | 70.1 |
| MS.gene054790.t1 | MTR_7g055790 | 39.640 | 111 | 48 | 2 | 1 | 110 | 59 | 151 | 6.55e-16 | 70.5 |
| MS.gene054790.t1 | MTR_1g033370 | 43.000 | 100 | 56 | 1 | 57 | 155 | 21 | 120 | 5.10e-15 | 67.4 |
| MS.gene054790.t1 | MTR_1g033790 | 43.333 | 90 | 50 | 1 | 64 | 152 | 22 | 111 | 7.47e-14 | 64.3 |
| MS.gene054790.t1 | MTR_6g005450 | 31.481 | 108 | 68 | 3 | 1 | 102 | 1 | 108 | 2.11e-12 | 63.9 |
| MS.gene054790.t1 | MTR_7g055940 | 34.314 | 102 | 40 | 2 | 1 | 102 | 1 | 75 | 2.55e-12 | 59.3 |
| MS.gene054790.t1 | MTR_3g093900 | 28.829 | 111 | 78 | 1 | 3 | 112 | 6 | 116 | 2.31e-11 | 60.8 |
| MS.gene054790.t1 | MTR_6g018920 | 28.704 | 108 | 71 | 3 | 1 | 102 | 1 | 108 | 2.92e-11 | 60.5 |
| MS.gene054790.t1 | MTR_1g114730 | 28.440 | 109 | 70 | 3 | 1 | 102 | 1 | 108 | 3.26e-11 | 60.5 |
| MS.gene054790.t1 | MTR_5g066180 | 34.513 | 113 | 57 | 3 | 1 | 113 | 1 | 96 | 6.30e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054790.t1 | AT5G26630 | 46.795 | 156 | 82 | 1 | 1 | 155 | 1 | 156 | 1.47e-39 | 133 |
| MS.gene054790.t1 | AT5G48670 | 47.436 | 156 | 81 | 1 | 1 | 155 | 1 | 156 | 6.76e-37 | 129 |
| MS.gene054790.t1 | AT1G65330 | 42.857 | 154 | 87 | 1 | 3 | 155 | 2 | 155 | 1.49e-36 | 127 |
| MS.gene054790.t1 | AT1G65300 | 40.909 | 154 | 90 | 1 | 3 | 155 | 2 | 155 | 5.93e-35 | 123 |
| MS.gene054790.t1 | AT3G05860 | 41.611 | 149 | 86 | 1 | 1 | 148 | 1 | 149 | 2.42e-34 | 119 |
| MS.gene054790.t1 | AT3G05860 | 41.611 | 149 | 86 | 1 | 1 | 148 | 1 | 149 | 8.63e-34 | 119 |
| MS.gene054790.t1 | AT3G05860 | 41.611 | 149 | 86 | 1 | 1 | 148 | 1 | 149 | 1.41e-33 | 119 |
| MS.gene054790.t1 | AT5G26650 | 37.908 | 153 | 94 | 1 | 4 | 155 | 2 | 154 | 1.93e-30 | 113 |
| MS.gene054790.t1 | AT5G27960 | 36.601 | 153 | 96 | 1 | 4 | 155 | 2 | 154 | 2.03e-28 | 107 |
| MS.gene054790.t1 | AT1G31630 | 31.126 | 151 | 102 | 2 | 3 | 152 | 2 | 151 | 1.85e-25 | 99.8 |
| MS.gene054790.t1 | AT1G22590 | 33.974 | 156 | 99 | 3 | 1 | 155 | 1 | 153 | 3.04e-23 | 90.1 |
| MS.gene054790.t1 | AT1G31640 | 34.000 | 150 | 97 | 2 | 3 | 151 | 2 | 150 | 6.05e-23 | 94.0 |
| MS.gene054790.t1 | AT2G28700 | 38.017 | 121 | 68 | 2 | 1 | 117 | 1 | 118 | 3.30e-21 | 88.2 |
| MS.gene054790.t1 | AT5G27810 | 43.333 | 90 | 50 | 1 | 29 | 117 | 1 | 90 | 5.99e-21 | 82.8 |
| MS.gene054790.t1 | AT5G26580 | 34.194 | 155 | 89 | 1 | 1 | 155 | 1 | 142 | 8.47e-21 | 87.0 |
| MS.gene054790.t1 | AT2G40210 | 36.496 | 137 | 81 | 3 | 1 | 134 | 1 | 134 | 3.19e-16 | 74.7 |
| MS.gene054790.t1 | AT5G06500 | 34.932 | 146 | 82 | 5 | 1 | 141 | 1 | 138 | 9.80e-16 | 72.0 |
| MS.gene054790.t1 | AT5G58890 | 35.417 | 96 | 55 | 2 | 1 | 89 | 1 | 96 | 1.87e-11 | 60.8 |
| MS.gene054790.t1 | AT1G47760 | 33.333 | 111 | 67 | 3 | 1 | 110 | 1 | 105 | 2.38e-11 | 59.3 |
| MS.gene054790.t1 | AT5G55690 | 29.167 | 120 | 78 | 2 | 1 | 113 | 1 | 120 | 4.97e-11 | 59.7 |
| MS.gene054790.t1 | AT5G55690 | 29.167 | 120 | 78 | 2 | 1 | 113 | 1 | 120 | 4.97e-11 | 59.7 |
Find 25 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTTTCATTGATGAGTTT+TGG | 0.227326 | 5.3:+34703005 | None:intergenic |
| CTCTAATGATTCGGCAAGAA+AGG | 0.394804 | 5.3:-34703185 | MS.gene054790:CDS |
| GAGCTGAACAATCTTATGAT+TGG | 0.404054 | 5.3:-34702887 | MS.gene054790:CDS |
| CTTTAGTAATCCTCTGCAAT+AGG | 0.413033 | 5.3:+34702954 | None:intergenic |
| GTACCTCTCAATTACCTGTC+TGG | 0.418841 | 5.3:+34703027 | None:intergenic |
| TCAGAAACAGATAATTCATC+TGG | 0.457651 | 5.3:+34702842 | None:intergenic |
| AGCTGAACAATCTTATGATT+GGG | 0.458403 | 5.3:-34702886 | MS.gene054790:CDS |
| AGAGAACTCACCATTCTTTG+TGG | 0.496533 | 5.3:-34703121 | MS.gene054790:CDS |
| GCTCCTTCTCACGATTATCA+TGG | 0.497017 | 5.3:+34702906 | None:intergenic |
| TGCTGGAATACCACAAAGAA+TGG | 0.514499 | 5.3:+34703111 | None:intergenic |
| TGAGAAAATTCTGAAGGACA+TGG | 0.544670 | 5.3:-34702795 | MS.gene054790:CDS |
| TTGGAAATTATAGCACATGC+TGG | 0.559487 | 5.3:+34703094 | None:intergenic |
| TCTGCAATAGGAAACTCTCT+TGG | 0.562811 | 5.3:+34702966 | None:intergenic |
| AGAGGTGTGGCCAGATCTAG+AGG | 0.572723 | 5.3:-34703053 | MS.gene054790:CDS |
| CAACGCCATGACTAGGAAGA+AGG | 0.575303 | 5.3:-34703224 | None:intergenic |
| GCTTATTGAGAAAATTCTGA+AGG | 0.584834 | 5.3:-34702801 | MS.gene054790:CDS |
| AAGAGAGTTTCCTATTGCAG+AGG | 0.589804 | 5.3:-34702964 | MS.gene054790:CDS |
| TTAATTCTAAAACAGAGGTG+TGG | 0.602716 | 5.3:-34703066 | MS.gene054790:CDS |
| TGCATCACAACGCCATGACT+AGG | 0.604632 | 5.3:-34703231 | None:intergenic |
| GATAACAAGATCGATGCACT+TGG | 0.609178 | 5.3:-34702773 | MS.gene054790:CDS |
| GAGGTGTGGCCAGATCTAGA+GGG | 0.615970 | 5.3:-34703052 | MS.gene054790:CDS |
| AAGCCATGATAATCGTGAGA+AGG | 0.628086 | 5.3:-34702909 | MS.gene054790:CDS |
| AGGTGTGGCCAGATCTAGAG+GGG | 0.656006 | 5.3:-34703051 | MS.gene054790:CDS |
| GGGCCAGACAGGTAATTGAG+AGG | 0.686598 | 5.3:-34703030 | MS.gene054790:CDS |
| GGTGTGGCCAGATCTAGAGG+GGG | 0.723497 | 5.3:-34703050 | MS.gene054790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACCTACAATAAAAGAAAAA+AGG | - | chr5.3:34702825-34702844 | MS.gene054790:CDS | 20.0% |
| !! | ACCTACAATAAAAGAAAAAA+GGG | - | chr5.3:34702826-34702845 | MS.gene054790:CDS | 20.0% |
| !!! | TGTTTTAGAATTAAAAGGAT+TGG | + | chr5.3:34702914-34702933 | None:intergenic | 20.0% |
| !! | TGCTTTTATCTCTAATGATT+CGG | - | chr5.3:34702792-34702811 | MS.gene054790:CDS | 25.0% |
| !!! | ACCCTTTTTTCTTTTATTGT+AGG | + | chr5.3:34702830-34702849 | None:intergenic | 25.0% |
| !!! | ACCTCTGTTTTAGAATTAAA+AGG | + | chr5.3:34702919-34702938 | None:intergenic | 25.0% |
| !!! | TCCTTTTAATTCTAAAACAG+AGG | - | chr5.3:34702915-34702934 | MS.gene054790:CDS | 25.0% |
| AGCTGAACAATCTTATGATT+GGG | - | chr5.3:34703100-34703119 | MS.gene054790:CDS | 30.0% | |
| GCTTATTGAGAAAATTCTGA+AGG | - | chr5.3:34703185-34703204 | MS.gene054790:CDS | 30.0% | |
| TCAGAAACAGATAATTCATC+TGG | + | chr5.3:34703147-34703166 | None:intergenic | 30.0% | |
| ! | CATCTTTCATTGATGAGTTT+TGG | + | chr5.3:34702984-34703003 | None:intergenic | 30.0% |
| !! | AAAAAAGGGTATCATCAAGA+AGG | - | chr5.3:34702840-34702859 | MS.gene054790:CDS | 30.0% |
| !! | TTAATTCTAAAACAGAGGTG+TGG | - | chr5.3:34702920-34702939 | MS.gene054790:CDS | 30.0% |
| CTTTAGTAATCCTCTGCAAT+AGG | + | chr5.3:34703035-34703054 | None:intergenic | 35.0% | |
| GAGCTGAACAATCTTATGAT+TGG | - | chr5.3:34703099-34703118 | MS.gene054790:CDS | 35.0% | |
| TTGGAAATTATAGCACATGC+TGG | + | chr5.3:34702895-34702914 | None:intergenic | 35.0% | |
| ! | TGAGAAAATTCTGAAGGACA+TGG | - | chr5.3:34703191-34703210 | MS.gene054790:CDS | 35.0% |
| AAGAGAGTTTCCTATTGCAG+AGG | - | chr5.3:34703022-34703041 | MS.gene054790:CDS | 40.0% | |
| AAGCCATGATAATCGTGAGA+AGG | - | chr5.3:34703077-34703096 | MS.gene054790:CDS | 40.0% | |
| AGAGAACTCACCATTCTTTG+TGG | - | chr5.3:34702865-34702884 | MS.gene054790:CDS | 40.0% | |
| GATAACAAGATCGATGCACT+TGG | - | chr5.3:34703213-34703232 | MS.gene054790:CDS | 40.0% | |
| TCTGCAATAGGAAACTCTCT+TGG | + | chr5.3:34703023-34703042 | None:intergenic | 40.0% | |
| TGCTGGAATACCACAAAGAA+TGG | + | chr5.3:34702878-34702897 | None:intergenic | 40.0% | |
| ! | CTCTAATGATTCGGCAAGAA+AGG | - | chr5.3:34702801-34702820 | MS.gene054790:CDS | 40.0% |
| !!! | TTGCTTTTGAAGTTGCTCTC+TGG | + | chr5.3:34703058-34703077 | None:intergenic | 40.0% |
| GCTCCTTCTCACGATTATCA+TGG | + | chr5.3:34703083-34703102 | None:intergenic | 45.0% | |
| GTACCTCTCAATTACCTGTC+TGG | + | chr5.3:34702962-34702981 | None:intergenic | 45.0% | |
| AGGTGTGGCCAGATCTAGAG+GGG | - | chr5.3:34702935-34702954 | MS.gene054790:CDS | 55.0% | |
| GAGGTGTGGCCAGATCTAGA+GGG | - | chr5.3:34702934-34702953 | MS.gene054790:CDS | 55.0% | |
| GGGCCAGACAGGTAATTGAG+AGG | - | chr5.3:34702956-34702975 | MS.gene054790:CDS | 55.0% | |
| ! | AGAGGTGTGGCCAGATCTAG+AGG | - | chr5.3:34702933-34702952 | MS.gene054790:CDS | 55.0% |
| AGATCTAGAGGGGGCCAGAC+AGG | - | chr5.3:34702945-34702964 | MS.gene054790:CDS | 60.0% | |
| GGTGTGGCCAGATCTAGAGG+GGG | - | chr5.3:34702936-34702955 | MS.gene054790:CDS | 60.0% | |
| TGTCTGGCCCCCTCTAGATC+TGG | + | chr5.3:34702946-34702965 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 34702769 | 34703239 | 34702769 | ID=MS.gene054790 |
| chr5.3 | mRNA | 34702769 | 34703239 | 34702769 | ID=MS.gene054790.t1;Parent=MS.gene054790 |
| chr5.3 | exon | 34702769 | 34703239 | 34702769 | ID=MS.gene054790.t1.exon1;Parent=MS.gene054790.t1 |
| chr5.3 | CDS | 34702769 | 34703239 | 34702769 | ID=cds.MS.gene054790.t1;Parent=MS.gene054790.t1 |
| Gene Sequence |
| Protein sequence |
