AlfalfaGEDB | MS.gene055194

M. sativa cultivar XinJiangDaYe / MS.gene055194

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055194.t1	XP_024626703.1	85.5	214	29	2	1	213	1	213	1.20E-97	365.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055194.t1	Q39266	32.6	132	80	4	9	136	5	131	3.5e-08	60.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055194.t1	A0A396GYM7	85.5	214	29	2	1	213	1	213	9.0e-98	365.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene055194.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055194.t1	MTR_2g067740	73.364	214	56	1	1	213	1	214	4.31e-99	286
MS.gene055194.t1	MTR_3g012010	39.474	114	54	5	58	160	98	207	1.22e-13	68.6
MS.gene055194.t1	MTR_3g009410	44.000	75	37	2	58	131	98	168	3.61e-13	67.4
MS.gene055194.t1	MTR_7g022980	34.400	125	62	5	55	174	63	172	4.66e-13	66.6
MS.gene055194.t1	MTR_5g008280	37.391	115	54	6	57	160	45	152	1.33e-12	66.2
MS.gene055194.t1	MTR_7g023010	33.600	125	63	5	55	174	63	172	3.81e-12	63.9
MS.gene055194.t1	MTR_7g022960	30.986	142	76	6	56	192	84	208	1.74e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055194.t1	AT1G24625	37.143	105	60	3	34	137	33	132	2.53e-12	63.9
MS.gene055194.t1	AT1G67030	40.000	85	41	4	56	138	37	113	8.69e-11	59.3
MS.gene055194.t1	AT5G01860	50.000	60	22	1	58	117	65	116	8.99e-11	59.7

Find 63 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAAATTTGGTGGCTATTT+GGG	0.249196	7.4:+40405602	None:intergenic
CTGAATAATAATGTGGGATT+TGG	0.253595	7.4:-40405715	MS.gene055194:CDS
CCATATCCCTTCACTGTTGC+AGG	0.257859	7.4:-40405847	MS.gene055194:CDS
CATTATTATTCAGTGGAGTT+TGG	0.280641	7.4:+40405725	None:intergenic
CAGCTGCAGGTTCCTTCATT+TGG	0.286157	7.4:-40405673	MS.gene055194:CDS
CAAGGAATGAGGTTGAAATT+TGG	0.293587	7.4:+40405590	None:intergenic
GTTGAAATTTGGTGGCTATT+TGG	0.298879	7.4:+40405601	None:intergenic
CCTGCAACAGTGAAGGGATA+TGG	0.307330	7.4:+40405847	None:intergenic
TATGGGTGATAAATTGAATA+TGG	0.313679	7.4:+40405865	None:intergenic
CATCCAAGTCCATGATAAAA+AGG	0.315893	7.4:+40405817	None:intergenic
GAAATTCAATTAGGAACGTT+TGG	0.323330	7.4:-40405910	MS.gene055194:CDS
CAATTAGGAACGTTTGGACT+TGG	0.342211	7.4:-40405904	MS.gene055194:CDS
ACGTCATTGTCTCTGAGTTT+AGG	0.351844	7.4:-40406096	MS.gene055194:CDS
GCAAGCTATGACAACCCATT+TGG	0.366612	7.4:-40405775	MS.gene055194:CDS
ATTATTATTCAGTGGAGTTT+GGG	0.370973	7.4:+40405726	None:intergenic
TCATAGCTTGCATGGGCAAC+AGG	0.381549	7.4:+40405787	None:intergenic
CAAGCACTCTTTCACGCCTA+TGG	0.392813	7.4:+40405947	None:intergenic
TTCTTGTTGCAAAACTTACA+TGG	0.408494	7.4:+40406009	None:intergenic
CAAGCTATGACAACCCATTT+GGG	0.409161	7.4:-40405774	MS.gene055194:CDS
GCTATTTGGGTCATCATGAT+TGG	0.436051	7.4:+40405615	None:intergenic
CTCATGGATCTTGAGGGATT+CGG	0.442013	7.4:+40406047	None:intergenic
TCAGCTCCTCATGGATCTTG+AGG	0.451104	7.4:+40406040	None:intergenic
AGTTTAGGAGGAGGGAGAAG+TGG	0.456414	7.4:-40406081	MS.gene055194:CDS
CAGCTCCTCATGGATCTTGA+GGG	0.457928	7.4:+40406041	None:intergenic
GGATGATCAATAACTCTGCT+TGG	0.462130	7.4:-40405753	MS.gene055194:CDS
ATGTCAGAAACACTACGATT+TGG	0.469253	7.4:-40406189	MS.gene055194:CDS
CTCTTAACAATCATGTGCTA+AGG	0.471735	7.4:-40405645	MS.gene055194:CDS
GAGTTATTGATCATCCCAAA+TGG	0.474296	7.4:+40405760	None:intergenic
CTGCAACAGTGAAGGGATAT+GGG	0.476975	7.4:+40405848	None:intergenic
AATTAGGAACGTTTGGACTT+GGG	0.482030	7.4:-40405903	MS.gene055194:CDS
GGTATTCCCTCAGCTCCTCA+TGG	0.496808	7.4:+40406031	None:intergenic
GGCACCTTGGTGTCCTCCCA+AGG	0.506085	7.4:+40405969	None:intergenic
GATGATCAATAACTCTGCTT+GGG	0.511350	7.4:-40405752	MS.gene055194:CDS
ATTAGGAACGTTTGGACTTG+GGG	0.514323	7.4:-40405902	MS.gene055194:CDS
TAGGGACAAATCAAGGAATG+AGG	0.515985	7.4:+40405579	None:intergenic
TGAACTTAATAATCAGCTGC+AGG	0.516698	7.4:-40405686	MS.gene055194:CDS
CTTGCATGGGCAACAGGTTG+AGG	0.519092	7.4:+40405793	None:intergenic
AGTTATTGATCATCCCAAAT+GGG	0.522275	7.4:+40405761	None:intergenic
ATGGGTTGTCATAGCTTGCA+TGG	0.525223	7.4:+40405779	None:intergenic
TTAGGAGGAGGGAGAAGTGG+AGG	0.528661	7.4:-40406078	MS.gene055194:CDS
TCTTAACAATCATGTGCTAA+GGG	0.533636	7.4:-40405644	MS.gene055194:CDS
GGAATGAGGTTGAAATTTGG+TGG	0.534438	7.4:+40405593	None:intergenic
AACTCCACTGAATAATAATG+TGG	0.547835	7.4:-40405722	MS.gene055194:CDS
AAGCACTCTTTCACGCCTAT+GGG	0.549509	7.4:+40405948	None:intergenic
TCTTGTTGCAAAACTTACAT+GGG	0.552429	7.4:+40406010	None:intergenic
AATCCCACATTATTATTCAG+TGG	0.569517	7.4:+40405718	None:intergenic
TTAAGAGCAACACCAAATGA+AGG	0.575359	7.4:+40405661	None:intergenic
TGACGACCCTGCAACAGTGA+AGG	0.582185	7.4:+40405840	None:intergenic
CAAGATCCATGAGGAGCTGA+GGG	0.584633	7.4:-40406037	MS.gene055194:CDS
TCATTGTCTCTGAGTTTAGG+AGG	0.586488	7.4:-40406093	MS.gene055194:CDS
TTTCACGCCTATGGGCACCT+TGG	0.587966	7.4:+40405956	None:intergenic
GACGACCCTGCAACAGTGAA+GGG	0.590012	7.4:+40405841	None:intergenic
GAGGACACCAAGGTGCCCAT+AGG	0.592853	7.4:-40405963	MS.gene055194:CDS
TCAAGATCCATGAGGAGCTG+AGG	0.596229	7.4:-40406038	MS.gene055194:CDS
TGTCTCTGAGTTTAGGAGGA+GGG	0.598792	7.4:-40406089	MS.gene055194:CDS
TCAAGTTCTCAAGCCTTGGG+AGG	0.602598	7.4:-40405982	MS.gene055194:CDS
TTGTCTCTGAGTTTAGGAGG+AGG	0.607557	7.4:-40406090	MS.gene055194:CDS
TGGGTTGTCATAGCTTGCAT+GGG	0.610755	7.4:+40405780	None:intergenic
CATATCCCTTCACTGTTGCA+GGG	0.613658	7.4:-40405846	MS.gene055194:CDS
ACTCCACTGAATAATAATGT+GGG	0.629245	7.4:-40405721	MS.gene055194:CDS
CTTAACAATCATGTGCTAAG+GGG	0.643060	7.4:-40405643	MS.gene055194:CDS
CAAGCCTTGGGAGGACACCA+AGG	0.644910	7.4:-40405973	MS.gene055194:CDS
CGAATCCCTCAAGATCCATG+AGG	0.735597	7.4:-40406046	MS.gene055194:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGAAAAAGAAATTCAATT+AGG	-	chr7.4:40405840-40405859	MS.gene055194:CDS	15.0%
!	ATTATTATTCAGTGGAGTTT+GGG	+	chr7.4:40406036-40406055	None:intergenic	25.0%
!	TATGGGTGATAAATTGAATA+TGG	+	chr7.4:40405897-40405916	None:intergenic	25.0%
	AACTCCACTGAATAATAATG+TGG	-	chr7.4:40406037-40406056	MS.gene055194:CDS	30.0%
	AATCCCACATTATTATTCAG+TGG	+	chr7.4:40406044-40406063	None:intergenic	30.0%
	ACTCCACTGAATAATAATGT+GGG	-	chr7.4:40406038-40406057	MS.gene055194:CDS	30.0%
	AGTTATTGATCATCCCAAAT+GGG	+	chr7.4:40406001-40406020	None:intergenic	30.0%
	CATTATTATTCAGTGGAGTT+TGG	+	chr7.4:40406037-40406056	None:intergenic	30.0%
	CTGAATAATAATGTGGGATT+TGG	-	chr7.4:40406044-40406063	MS.gene055194:CDS	30.0%
	GAAATTCAATTAGGAACGTT+TGG	-	chr7.4:40405849-40405868	MS.gene055194:CDS	30.0%
	TCTTAACAATCATGTGCTAA+GGG	-	chr7.4:40406115-40406134	MS.gene055194:CDS	30.0%
	TCTTGTTGCAAAACTTACAT+GGG	+	chr7.4:40405752-40405771	None:intergenic	30.0%
	TTCTTGTTGCAAAACTTACA+TGG	+	chr7.4:40405753-40405772	None:intergenic	30.0%
!	TTGAAATTTGGTGGCTATTT+GGG	+	chr7.4:40406160-40406179	None:intergenic	30.0%
!!!	AAAGTTTTAGGGACAAATCA+AGG	+	chr7.4:40406190-40406209	None:intergenic	30.0%
	AATTAGGAACGTTTGGACTT+GGG	-	chr7.4:40405856-40405875	MS.gene055194:CDS	35.0%
	ATGTCAGAAACACTACGATT+TGG	-	chr7.4:40405570-40405589	MS.gene055194:CDS	35.0%
	CAAGGAATGAGGTTGAAATT+TGG	+	chr7.4:40406172-40406191	None:intergenic	35.0%
	CATCCAAGTCCATGATAAAA+AGG	+	chr7.4:40405945-40405964	None:intergenic	35.0%
	CTCTTAACAATCATGTGCTA+AGG	-	chr7.4:40406114-40406133	MS.gene055194:CDS	35.0%
	CTTAACAATCATGTGCTAAG+GGG	-	chr7.4:40406116-40406135	MS.gene055194:CDS	35.0%
	GAGTTATTGATCATCCCAAA+TGG	+	chr7.4:40406002-40406021	None:intergenic	35.0%
	GATGATCAATAACTCTGCTT+GGG	-	chr7.4:40406007-40406026	MS.gene055194:CDS	35.0%
	TGAACTTAATAATCAGCTGC+AGG	-	chr7.4:40406073-40406092	MS.gene055194:CDS	35.0%
	TTAAGAGCAACACCAAATGA+AGG	+	chr7.4:40406101-40406120	None:intergenic	35.0%
!	CTTTTTATCATGGACTTGGA+TGG	-	chr7.4:40405943-40405962	MS.gene055194:CDS	35.0%
!	GTTGAAATTTGGTGGCTATT+TGG	+	chr7.4:40406161-40406180	None:intergenic	35.0%
!	TCACCTTTTTATCATGGACT+TGG	-	chr7.4:40405939-40405958	MS.gene055194:CDS	35.0%
!!!	TTTTCAAGTTCTCAAGCCTT+GGG	-	chr7.4:40405774-40405793	MS.gene055194:CDS	35.0%
	ACGTCATTGTCTCTGAGTTT+AGG	-	chr7.4:40405663-40405682	MS.gene055194:CDS	40.0%
	ATTAGGAACGTTTGGACTTG+GGG	-	chr7.4:40405857-40405876	MS.gene055194:CDS	40.0%
	CAAAAAGCTAAAGCGCAAGA+TGG	-	chr7.4:40405614-40405633	MS.gene055194:CDS	40.0%
	CAAGCTATGACAACCCATTT+GGG	-	chr7.4:40405985-40406004	MS.gene055194:CDS	40.0%
	CAATTAGGAACGTTTGGACT+TGG	-	chr7.4:40405855-40405874	MS.gene055194:CDS	40.0%
	GCTATTTGGGTCATCATGAT+TGG	+	chr7.4:40406147-40406166	None:intergenic	40.0%
	GGAATGAGGTTGAAATTTGG+TGG	+	chr7.4:40406169-40406188	None:intergenic	40.0%
	GGATGATCAATAACTCTGCT+TGG	-	chr7.4:40406006-40406025	MS.gene055194:CDS	40.0%
	TAGGGACAAATCAAGGAATG+AGG	+	chr7.4:40406183-40406202	None:intergenic	40.0%
	TCATTGTCTCTGAGTTTAGG+AGG	-	chr7.4:40405666-40405685	MS.gene055194:CDS	40.0%
!!!	GTTTTCAAGTTCTCAAGCCT+TGG	-	chr7.4:40405773-40405792	MS.gene055194:CDS	40.0%
	AAGCACTCTTTCACGCCTAT+GGG	+	chr7.4:40405814-40405833	None:intergenic	45.0%
	CATATCCCTTCACTGTTGCA+GGG	-	chr7.4:40405913-40405932	MS.gene055194:CDS	45.0%
	CTCATGGATCTTGAGGGATT+CGG	+	chr7.4:40405715-40405734	None:intergenic	45.0%
	CTGCAACAGTGAAGGGATAT+GGG	+	chr7.4:40405914-40405933	None:intergenic	45.0%
	GCAAGCTATGACAACCCATT+TGG	-	chr7.4:40405984-40406003	MS.gene055194:CDS	45.0%
	TGTCTCTGAGTTTAGGAGGA+GGG	-	chr7.4:40405670-40405689	MS.gene055194:CDS	45.0%
	TTGTCTCTGAGTTTAGGAGG+AGG	-	chr7.4:40405669-40405688	MS.gene055194:CDS	45.0%
!	ATGGGTTGTCATAGCTTGCA+TGG	+	chr7.4:40405983-40406002	None:intergenic	45.0%
!	GGGTCGTCACCTTTTTATCA+TGG	-	chr7.4:40405933-40405952	MS.gene055194:CDS	45.0%
!	TGGGTTGTCATAGCTTGCAT+GGG	+	chr7.4:40405982-40406001	None:intergenic	45.0%
	AGTTTAGGAGGAGGGAGAAG+TGG	-	chr7.4:40405678-40405697	MS.gene055194:CDS	50.0%
	CAAGATCCATGAGGAGCTGA+GGG	-	chr7.4:40405722-40405741	MS.gene055194:CDS	50.0%
	CAAGCACTCTTTCACGCCTA+TGG	+	chr7.4:40405815-40405834	None:intergenic	50.0%
	CAGCTCCTCATGGATCTTGA+GGG	+	chr7.4:40405721-40405740	None:intergenic	50.0%
	CAGCTGCAGGTTCCTTCATT+TGG	-	chr7.4:40406086-40406105	MS.gene055194:CDS	50.0%
	CCATATCCCTTCACTGTTGC+AGG	-	chr7.4:40405912-40405931	MS.gene055194:CDS	50.0%
	CCTGCAACAGTGAAGGGATA+TGG	+	chr7.4:40405915-40405934	None:intergenic	50.0%
	CGAATCCCTCAAGATCCATG+AGG	-	chr7.4:40405713-40405732	MS.gene055194:CDS	50.0%
	TCAAGATCCATGAGGAGCTG+AGG	-	chr7.4:40405721-40405740	MS.gene055194:CDS	50.0%
	TCAAGTTCTCAAGCCTTGGG+AGG	-	chr7.4:40405777-40405796	MS.gene055194:CDS	50.0%
	TCAGCTCCTCATGGATCTTG+AGG	+	chr7.4:40405722-40405741	None:intergenic	50.0%
!	TCATAGCTTGCATGGGCAAC+AGG	+	chr7.4:40405975-40405994	None:intergenic	50.0%
	CTTGCATGGGCAACAGGTTG+AGG	+	chr7.4:40405969-40405988	None:intergenic	55.0%
	GACGACCCTGCAACAGTGAA+GGG	+	chr7.4:40405921-40405940	None:intergenic	55.0%
	GGTATTCCCTCAGCTCCTCA+TGG	+	chr7.4:40405731-40405750	None:intergenic	55.0%
	TGACGACCCTGCAACAGTGA+AGG	+	chr7.4:40405922-40405941	None:intergenic	55.0%
	TTAGGAGGAGGGAGAAGTGG+AGG	-	chr7.4:40405681-40405700	MS.gene055194:CDS	55.0%
	TTTCACGCCTATGGGCACCT+TGG	+	chr7.4:40405806-40405825	None:intergenic	55.0%
	CAAGCCTTGGGAGGACACCA+AGG	-	chr7.4:40405786-40405805	MS.gene055194:CDS	60.0%
!	GAGGACACCAAGGTGCCCAT+AGG	-	chr7.4:40405796-40405815	MS.gene055194:CDS	60.0%
!!	GGCACCTTGGTGTCCTCCCA+AGG	+	chr7.4:40405793-40405812	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	40405570	40406211	40405570	ID=MS.gene055194
chr7.4	mRNA	40405570	40406211	40405570	ID=MS.gene055194.t1;Parent=MS.gene055194
chr7.4	exon	40405570	40406211	40405570	ID=MS.gene055194.t1.exon1;Parent=MS.gene055194.t1
chr7.4	CDS	40405570	40406211	40405570	ID=cds.MS.gene055194.t1;Parent=MS.gene055194.t1

Gene Sequence

>MS.gene055194

ATGTCAGAAACACTACGATTTGGTGATGATGTTACAAGAGATAACAAAAAGCTAAAGCGCAAGATGGATGATGCTATTTCTGCTGATTCAGAGACGTCATTGTCTCTGAGTTTAGGAGGAGGGAGAAGTGGAGGATCTTCATCCGAATCCCTCAAGATCCATGAGGAGCTGAGGGAATACCCATGTAAGTTTTGCAACAAGAAGTTTTCAAGTTCTCAAGCCTTGGGAGGACACCAAGGTGCCCATAGGCGTGAAAGAGTGCTTGCGAGAATTGAAAAAGAAATTCAATTAGGAACGTTTGGACTTGGGGATCATCATTTGTGTCCATATTCAATTTATCACCCATATCCCTTCACTGTTGCAGGGTCGTCACCTTTTTATCATGGACTTGGATGGCCTCAACCTGTTGCCCATGCAAGCTATGACAACCCATTTGGGATGATCAATAACTCTGCTTGGGCTACCCAAACTCCACTGAATAATAATGTGGGATTTGGAAATTATGAACTTAATAATCAGCTGCAGGTTCCTTCATTTGGTGTTGCTCTTAACAATCATGTGCTAAGGGGAAATACCAATCATGATGACCCAAATAGCCACCAAATTTCAACCTCATTCCTTGATTTGTCCCTAAAACTTTAG

Protein sequence

>MS.gene055194.t1

MSETLRFGDDVTRDNKKLKRKMDDAISADSETSLSLSLGGGRSGGSSSESLKIHEELREYPCKFCNKKFSSSQALGGHQGAHRRERVLARIEKEIQLGTFGLGDHHLCPYSIYHPYPFTVAGSSPFYHGLGWPQPVAHASYDNPFGMINNSAWATQTPLNNNVGFGNYELNNQLQVPSFGVALNNHVLRGNTNHDDPNSHQISTSFLDLSLKL