AlfalfaGEDB | MS.gene05533

M. sativa cultivar XinJiangDaYe / MS.gene05533

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05533.t1	RHN52888.1	88.6	361	41	0	1	361	1	361	6.60E-184	653.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05533.t1	Q8VWJ2	56.0	75	32	1	140	214	158	231	9.8e-19	95.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05533.t1	A0A072UCB0	88.6	361	41	0	1	361	53	413	4.8e-184	653.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene05533.t1	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05533.t1	MTR_6g086555	88.643	361	41	0	1	361	53	413	0.0	660
MS.gene05533.t1	MTR_1g078260	46.970	396	174	9	1	361	90	484	3.44e-101	307
MS.gene05533.t1	MTR_7g080020	41.353	133	76	2	103	235	443	573	1.61e-24	105
MS.gene05533.t1	MTR_7g080020	41.333	75	42	2	155	229	281	353	5.22e-13	70.9
MS.gene05533.t1	MTR_7g080000	46.226	106	55	2	109	214	388	491	9.00e-24	103
MS.gene05533.t1	MTR_7g080000	41.558	77	43	2	153	229	233	307	5.67e-13	70.5
MS.gene05533.t1	MTR_7g100510	34.868	152	94	3	80	231	291	437	2.94e-23	100
MS.gene05533.t1	MTR_7g100510	46.667	75	38	2	155	229	190	262	6.53e-14	72.8
MS.gene05533.t1	MTR_5g042920	57.333	75	31	1	140	214	306	379	3.26e-23	100
MS.gene05533.t1	MTR_5g042920	39.450	109	55	5	126	230	119	220	1.26e-13	72.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05533.t1	AT5G41570	53.425	73	33	1	139	211	83	154	1.21e-22	94.0
MS.gene05533.t1	AT1G13960	54.667	75	33	1	140	214	395	468	1.68e-22	99.0
MS.gene05533.t1	AT1G13960	46.575	73	36	3	148	219	222	292	3.44e-13	70.9
MS.gene05533.t1	AT4G18170	56.000	75	32	1	140	214	158	231	1.93e-22	96.7
MS.gene05533.t1	AT1G29860	56.757	74	31	1	141	214	123	195	1.97e-22	95.9
MS.gene05533.t1	AT1G13960	54.667	75	33	1	140	214	242	315	2.61e-22	97.1
MS.gene05533.t1	AT1G13960	46.575	73	36	3	148	219	69	139	1.18e-12	68.9
MS.gene05533.t1	AT2G03340	54.667	75	33	1	140	214	401	474	3.52e-22	98.2
MS.gene05533.t1	AT2G03340	47.761	67	32	3	149	214	244	308	1.31e-12	69.3
MS.gene05533.t1	AT1G64000	53.521	71	32	1	141	211	101	170	3.69e-22	93.2

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Find 75 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCATGAAGTTCTGGTAG+AGG	0.540635	6.2:-84783792	None:intergenic
AACCTGTAATGTGAGAAAAC+AGG	0.528589	6.2:+84783380	MS.gene05533:CDS
AAGATTCATAACTTTCAGAG+CGG	0.728852	6.2:-84783596	None:intergenic
AAGCAACATCATTCCTTCAA+TGG	0.416911	6.2:-84782372	None:intergenic
AAGGAAATGCTAAATACCCT+AGG	0.580382	6.2:+84782680	MS.gene05533:CDS
AAGTGAATGATGGTTATCGT+TGG	0.626452	6.2:+84782632	MS.gene05533:CDS
AATACCATGATGATGCCTTA+TGG	0.355278	6.2:+84783672	MS.gene05533:CDS
AATACTGGGAACAGCTTGAG+AGG	0.558169	6.2:-84783748	None:intergenic
AATGAAGACAAAGTGAATGA+TGG	0.529985	6.2:+84782622	MS.gene05533:CDS
ACAACACTTGATCAAGGACA+TGG	0.685805	6.2:+84782149	MS.gene05533:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATTCATAACTTTCAGAG+CGG	-	chr6.2:84783599-84783618	None:intergenic	30.0%
AATGAAGACAAAGTGAATGA+TGG	+	chr6.2:84782622-84782641	MS.gene05533:CDS	30.0%
CTTCTAATTATAAAGAACGG+GGG	-	chr6.2:84782875-84782894	None:intergenic	30.0%
TAATTGCATTACTTTGCACA+TGG	-	chr6.2:84782193-84782212	None:intergenic	30.0%
TATGAAGGCAAACATAATCA+CGG	+	chr6.2:84783444-84783463	MS.gene05533:CDS	30.0%
TCCTAATTATTATGCAATGC+AGG	+	chr6.2:84782512-84782531	MS.gene05533:intron	30.0%
TCCTGCATTGCATAATAATT+AGG	-	chr6.2:84782516-84782535	None:intergenic	30.0%
TTATTGACCATGTTCAAGAA+AGG	-	chr6.2:84783654-84783673	None:intergenic	30.0%
AACCTGTAATGTGAGAAAAC+AGG	+	chr6.2:84783380-84783399	MS.gene05533:CDS	35.0%
AAGCAACATCATTCCTTCAA+TGG	-	chr6.2:84782375-84782394	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	84782080	84783917	84782080	ID=MS.gene05533
chr6.2	mRNA	84782080	84783917	84782080	ID=MS.gene05533.t1;Parent=MS.gene05533
chr6.2	exon	84782080	84782435	84782080	ID=MS.gene05533.t1.exon1;Parent=MS.gene05533.t1
chr6.2	CDS	84782080	84782435	84782080	ID=cds.MS.gene05533.t1;Parent=MS.gene05533.t1
chr6.2	exon	84782534	84782701	84782534	ID=MS.gene05533.t1.exon2;Parent=MS.gene05533.t1
chr6.2	CDS	84782534	84782701	84782534	ID=cds.MS.gene05533.t1;Parent=MS.gene05533.t1
chr6.2	exon	84783356	84783917	84783356	ID=MS.gene05533.t1.exon3;Parent=MS.gene05533.t1
chr6.2	CDS	84783356	84783917	84783356	ID=cds.MS.gene05533.t1;Parent=MS.gene05533.t1

Gene Sequence

>MS.gene05533

ATGGCAACGGGAGGAGGTGTAGAACTCCAAGATGAAGTTCAACATCCAATTTCAAATATTTCCTTCCCAACAACACTTGATCAAGGACATGGTGCAGAAAAAGAACAAGTCCATGTGCAAAGTAATGCAATTACGCTAAACGCTGAAATTCTAAAGGCTAGGAAAGGAAAGATGCCAATGAATTATCGTGAAGATGCACCTACCCAAGAAGTTGTTGCAGCTGATGAAGCAAAAACGGTATCCTTAGATGAACAACAAGTTACAAATACAACTATTGGTGTTGAAGCAAGTTCCATTGAAGGAATGATGTTGCTTCAACATAATGATACTAGTGCTAGTGGATCTTCTTCACAAAGGTTTTATAATTATTCAATATTGTAAATATATATTTGTTTCAAACAAAAAATGGCTAACTTTTTTTGTTTTTTCATTTCCTAATTATTATGCAATGCAGGAAGAGGAAAACTGACTTCTCTGAACACTATAAACTCCCTTGCACCGTGATAAATGTTGCGCCAAAAGTAGTGTTCAGAATTGAAATCAATGAAGACAAAGTGAATGATGGTTATCGTTGGCATAAGTATGGACGCAAGGAGATCAAAGGAAATGCTAAATACCCTAGGTAATCATTTACCTTTTTCCCTGGTGTCAATGCAAGTATGTGTATATTTATAAATGTTTTTCTTTTTAATGCATTATTCGCCTTGGTGTAGATATGAAAGAGGAATATATGATTCAAGACATCATGGACTAGTACTTAAACTTTTTCTAAAACACCAACAACTACTGACGCCCCCGTTCTTTATAATTAGAAGTTTATAGTTCTCATTGTTTTCATAGCCAAGACAGTGACATAAATTTATATATATTATCATTTCGTTTATTGTCTCAAATGTGACAACTAGAAAATTATTGAACTTCTACTTTTAAGAACTAACATCAATCATCATTATACTTTGAGATGATTTTAAGGTATTGATCAACAATTGACCCCTGCTAAAAGTGATTTATCGTAAAGATTACATGCAATTTGATATTAAATTTGTGGGGACATACACGTATATATGTCCCTTAATTATCGATTTTTCACCAAAATTTATTATTTAATTATAAGTAGTTTTCACTATACTTTATTTGTGCACACACATGTTTTAGTATATATATAGTTTCTTATCATGATGTAAATATTGTTCATTGGAATTAAGGTATTTGTACCATGTAGCATATACATAAATATGTTTTGAAGCATGATTAATTAATATTGTATGCATTATAGGGGATACTACAGGTGTACAACAAAAACCTGTAATGTGAGAAAACAGGTGGAAAGGGATGCACAGAATCCGAAATATGTAATTGTTTCATATGAAGGCAAACATAATCACGGATTCCCTATCATCACAAAAAAAAATCCAACAAAGTCAAGTACTACACTGACAACAGTAACACGTAGGAATGCTAGCATTGTATCGCCACCTTTTGCATTGCCTATGACCCAACCTTTCACACCTCCTCACCGCTCTGAAAGTTATGAATCTTATGGTAAGAACTGTACTTGGCCTAATAATAATCCTTTCTTGAACATGGTCAATAATACCATGATGATGCCTTATGGATATATTAATGGACAACATTTTCGAGATTGTTATGCTGCTCCTTCAATTGCTGCCTCTCAAGCTGTTCCCAGTATTGGTGGATTTCAAATATCTCCACCTCTACCAGAACTTCATGATTTTCTTCGACTTAATGGGTACAATATTGCTGCTGCTACAATGTTTCCTTTTGAAGGTGCTACACCAATTAATCATGAAGCCAACGACAGTCCTAGTAATGAATGA

Protein sequence

>MS.gene05533.t1

MATGGGVELQDEVQHPISNISFPTTLDQGHGAEKEQVHVQSNAITLNAEILKARKGKMPMNYREDAPTQEVVAADEAKTVSLDEQQVTNTTIGVEASSIEGMMLLQHNDTSASGSSSQRKRKTDFSEHYKLPCTVINVAPKVVFRIEINEDKVNDGYRWHKYGRKEIKGNAKYPRGYYRCTTKTCNVRKQVERDAQNPKYVIVSYEGKHNHGFPIITKKNPTKSSTTLTTVTRRNASIVSPPFALPMTQPFTPPHRSESYESYGKNCTWPNNNPFLNMVNNTMMMPYGYINGQHFRDCYAAPSIAASQAVPSIGGFQISPPLPELHDFLRLNGYNIAAATMFPFEGATPINHEANDSPSNE