AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055489

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055489.t1 MTR_3g099370 96.804 876 27 1 1 876 1 875 0.0 1754
MS.gene055489.t1 MTR_7g106990 38.904 365 210 8 11 372 87 441 4.99e-75 253
MS.gene055489.t1 MTR_3g077420 22.000 300 163 11 136 379 16 300 9.50e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055489.t1 AT1G78280 59.908 873 305 11 11 876 6 840 0.0 1067
MS.gene055489.t1 AT5G06550 37.875 367 215 8 12 376 73 428 2.40e-74 253
MS.gene055489.t1 AT5G63080 26.690 281 164 11 133 379 7 279 8.33e-19 90.9

Find 211 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCTTTGTTAAGATTC+TGG 0.145954 3.2:-82668708 None:intergenic
GCATGGAATACTCTTCTTTC+TGG 0.157901 3.2:+82664684 MS.gene055489:CDS
TGATTTCAGTGATCTTTCTA+TGG 0.200906 3.2:+82669484 MS.gene055489:CDS
GTCTACATCTTTCAGAGTTT+TGG 0.213866 3.2:-82666436 None:intergenic
GGACTGGAAGCATCGCTTTA+TGG 0.213881 3.2:+82667941 MS.gene055489:CDS
CATCCAGAAGCTATAATTTA+TGG 0.214640 3.2:+82666490 MS.gene055489:CDS
GATATAAATGAATTATTTAA+AGG 0.220719 3.2:-82668590 None:intergenic
CGAATTTGTATGTTTGGATA+TGG 0.225637 3.2:+82666272 MS.gene055489:CDS
CTCTTCTTTCTGGCCGTAAA+AGG 0.234128 3.2:+82664694 MS.gene055489:CDS
TCCTATAGGTGGCTTATAAT+TGG 0.239011 3.2:+82664612 MS.gene055489:CDS
CACCCTCAGGTCATGCTAAA+TGG 0.250190 3.2:+82664181 MS.gene055489:intron
CATATTAGATCTCTCATATC+TGG 0.253644 3.2:-82666657 None:intergenic
ACGTGGATGGTTTCCCGTTT+GGG 0.269531 3.2:+82668223 MS.gene055489:CDS
TTGGATACACACTGACATTA+TGG 0.277456 3.2:+82669328 MS.gene055489:CDS
TAACTTCGAATTTGTATGTT+TGG 0.284606 3.2:+82666266 MS.gene055489:CDS
AACAAACTTGGCCTCCTTCC+TGG 0.289607 3.2:+82668529 MS.gene055489:CDS
TTGTTTGAGTATAGGAATAC+TGG 0.294582 3.2:+82668261 MS.gene055489:CDS
TCAATCAGTTTGCTAGGAAT+TGG 0.295536 3.2:-82669119 None:intergenic
TTTCCATAAATTATAGCTTC+TGG 0.297974 3.2:-82666493 None:intergenic
CAGATATGAGAGATCTAATA+TGG 0.307979 3.2:+82666658 MS.gene055489:CDS
TGGTTTCCCGTTTGGGGTAT+GGG 0.311759 3.2:+82668230 MS.gene055489:CDS
GACATAATCCTTGAACTTCA+TGG 0.326012 3.2:-82664311 None:intergenic
CTCGCGTCGCTTGCGTTAGC+AGG 0.327432 3.2:+82662280 MS.gene055489:CDS
CATCGCTTAATTATCAAATA+TGG 0.329184 3.2:-82668333 None:intergenic
ATGGTTTCCCGTTTGGGGTA+TGG 0.336226 3.2:+82668229 MS.gene055489:CDS
AACGTGGATGGTTTCCCGTT+TGG 0.339996 3.2:+82668222 MS.gene055489:CDS
CATCCATAGTGGTTCTTCAT+TGG 0.346934 3.2:-82662909 None:intergenic
TGGCACTCTTTGTACTTCTC+TGG 0.357779 3.2:-82663112 None:intergenic
ATCCCAACTTAAATTGGTAT+AGG 0.361855 3.2:-82668145 None:intergenic
GAGAATGGTTGTCCAAGCTT+TGG 0.365534 3.2:+82666625 MS.gene055489:CDS
GATCAGCTGTTACAAAACTA+TGG 0.367650 3.2:+82664244 MS.gene055489:CDS
GAAAGGCTTCCTGTTGGTTC+GGG 0.369837 3.2:+82666868 MS.gene055489:CDS
TTTCGTCGAAAATATATAAT+GGG 0.370958 3.2:-82664363 None:intergenic
CGAAAGGCTTCCTGTTGGTT+CGG 0.372369 3.2:+82666867 MS.gene055489:CDS
CATGGCCTGCCAGGCAGAAA+TGG 0.378327 3.2:+82664215 MS.gene055489:CDS
AATGTAAAGGCTGCTGTCTA+TGG 0.380107 3.2:-82663132 None:intergenic
ATCAAACAATGCAGCAGAAT+GGG 0.387429 3.2:+82668676 MS.gene055489:CDS
TAGCCGGTAACTCAAGTATA+TGG 0.392078 3.2:+82668310 MS.gene055489:CDS
TGGCCATATACTTGAGTTAC+CGG 0.392860 3.2:-82668313 None:intergenic
TGGATGACTCTATGCCTAAA+AGG 0.397240 3.2:+82662926 MS.gene055489:CDS
CCTCCTGGAAAAGTGCCTTT+AGG 0.405897 3.2:+82665885 MS.gene055489:CDS
TGCCTAAAAGGAGGAGCATC+TGG 0.408109 3.2:+82662938 MS.gene055489:CDS
GGGCAACAGCATTGGTCAAC+GGG 0.409974 3.2:+82666596 MS.gene055489:CDS
GGTAGTTGTGTACATTCAAT+TGG 0.411189 3.2:-82666150 None:intergenic
GTATAGGAATACTGGGATTC+CGG 0.412569 3.2:+82668269 MS.gene055489:CDS
ACAAACTTGGCCTCCTTCCT+GGG 0.419271 3.2:+82668530 MS.gene055489:CDS
GTTGTCCATTTCTGCCTGGC+AGG 0.425101 3.2:-82664220 None:intergenic
GAAACATATTCCTAGTGTTA+TGG 0.425446 3.2:+82668092 MS.gene055489:CDS
CAGGCATTCTAACCACTTGC+CGG 0.427854 3.2:-82666800 None:intergenic
ATATTGAACGTGAATTAAAC+TGG 0.429371 3.2:+82668615 MS.gene055489:CDS
GGTGTTGAGATTGGACATAT+TGG 0.431128 3.2:+82670724 MS.gene055489:CDS
AATCCTATACCAATTTAAGT+TGG 0.431151 3.2:+82668142 MS.gene055489:CDS
TGGCGAATACATGGCCTGCC+AGG 0.433299 3.2:+82664206 MS.gene055489:CDS
GATTTCAGTGATCTTTCTAT+GGG 0.433748 3.2:+82669485 MS.gene055489:CDS
ACATGCCCCTAGCTTATTGA+AGG 0.436977 3.2:+82664524 MS.gene055489:CDS
GCAAGCAATTGTTTGAGTAT+AGG 0.439732 3.2:+82668253 MS.gene055489:CDS
ATCCTATACCAATTTAAGTT+GGG 0.441548 3.2:+82668143 MS.gene055489:CDS
ATCAGTTGTCCATTTCTGCC+TGG 0.443048 3.2:-82664224 None:intergenic
GAAGCATCGCTTTATGGTTT+AGG 0.443732 3.2:+82667947 MS.gene055489:CDS
CACAGAGATCTGGTGCCTCT+TGG 0.445712 3.2:+82664637 MS.gene055489:CDS
AAAGATCTCCATGAAGTTCA+AGG 0.447205 3.2:+82664303 MS.gene055489:CDS
TGCTTGCCCCATACCCCAAA+CGG 0.447802 3.2:-82668237 None:intergenic
TGTAAATAGGTTTATCTAGT+GGG 0.450022 3.2:+82667887 MS.gene055489:intron
GCACCTGGTTATCGTCATAA+AGG 0.453039 3.2:+82666294 MS.gene055489:CDS
TTCTTCAATACAAAGGCTCT+TGG 0.456556 3.2:+82662963 MS.gene055489:CDS
TATCCAATGAAGAACCACTA+TGG 0.457458 3.2:+82662906 MS.gene055489:CDS
CTGAAATCAAGGAGGTAACT+AGG 0.465174 3.2:-82669470 None:intergenic
GCACTTCTAAACCCTGCTCT+TGG 0.465947 3.2:+82669309 MS.gene055489:CDS
TTCCATGCACTTGTAAGAGC+TGG 0.466147 3.2:-82664669 None:intergenic
TTCCTAGTGTTATGGCCAGT+GGG 0.468965 3.2:+82668100 MS.gene055489:CDS
TAGAAAGATCACTGAAATCA+AGG 0.469489 3.2:-82669481 None:intergenic
GTCAGTGTGTATCCAAGAGC+AGG 0.469541 3.2:-82669321 None:intergenic
AAGTATACCACTTGGTCAAA+CGG 0.469956 3.2:-82668745 None:intergenic
CAGATAAACGACCATCCTAT+AGG 0.470762 3.2:+82664598 MS.gene055489:CDS
CCTTTCGTCATCTGTGGGAG+GGG 0.475975 3.2:-82666851 None:intergenic
TTGAAGCCAACTCCCCTCTC+AGG 0.479773 3.2:+82668070 MS.gene055489:CDS
TCTAGAACCTGCAGTGATGA+TGG 0.481252 3.2:-82665957 None:intergenic
TCCAATTATAAGCCACCTAT+AGG 0.486415 3.2:-82664613 None:intergenic
ATAATTGGACCACAGAGATC+TGG 0.489173 3.2:+82664627 MS.gene055489:CDS
GCCTTTCGTCATCTGTGGGA+GGG 0.490405 3.2:-82666852 None:intergenic
TGTGGAGAGTTGAGGAACTC+TGG 0.494165 3.2:+82670749 MS.gene055489:CDS
GTGGGGAATGGTGTTGAGAT+TGG 0.496345 3.2:+82670715 MS.gene055489:intron
GAATTATTTAAAGGTGGATG+TGG 0.496530 3.2:-82668581 None:intergenic
AATCAAACAATGCAGCAGAA+TGG 0.499094 3.2:+82668675 MS.gene055489:CDS
CAATTTATGTTCCGAGTGGA+TGG 0.499365 3.2:+82666181 MS.gene055489:CDS
GGTTTCCCGTTTGGGGTATG+GGG 0.499368 3.2:+82668231 MS.gene055489:CDS
ACATGCACCGTCACACCTAA+AGG 0.501322 3.2:-82665900 None:intergenic
AGGTGGGCATTATATCCTCC+TGG 0.509762 3.2:+82665870 MS.gene055489:intron
GCTTGCCCCATACCCCAAAC+GGG 0.510621 3.2:-82668236 None:intergenic
ATGTTTCCTGAGAGGGGAGT+TGG 0.512138 3.2:-82668076 None:intergenic
TGGAGCTCGGGCAACAGCAT+TGG 0.513686 3.2:+82666588 MS.gene055489:CDS
TATACCAATTTAAGTTGGGA+TGG 0.514509 3.2:+82668147 MS.gene055489:CDS
TAAATGGACTGGCGAATACA+TGG 0.516680 3.2:+82664197 MS.gene055489:CDS
ATTGAGCAGAGGATTTCATC+GGG 0.517061 3.2:-82662228 None:intergenic
AATTTAAGTTGGGATGGAAA+AGG 0.517463 3.2:+82668153 MS.gene055489:CDS
CAGTAATCCTTCAATAAGCT+AGG 0.518812 3.2:-82664531 None:intergenic
AATTGAGCAGAGGATTTCAT+CGG 0.521661 3.2:-82662229 None:intergenic
AATAAATGACATCTCAATGA+AGG 0.522634 3.2:+82663371 MS.gene055489:CDS
CAAGTATGACATGAAGAAAC+CGG 0.525208 3.2:+82663404 MS.gene055489:CDS
TAAGTTACTCTGATTTGTCA+AGG 0.527231 3.2:+82666400 MS.gene055489:CDS
TTGATAATTAAGCGATGTGA+GGG 0.528989 3.2:+82668339 MS.gene055489:CDS
TAGGAATATGTTTCCTGAGA+GGG 0.530545 3.2:-82668083 None:intergenic
CGGGCAACAGCATTGGTCAA+CGG 0.534919 3.2:+82666595 MS.gene055489:CDS
AGGCATAGAGTCATCCATAG+TGG 0.536115 3.2:-82662920 None:intergenic
ATAAATGAATTATTTAAAGG+TGG 0.536575 3.2:-82668587 None:intergenic
TATGGATGATAATATTTACA+TGG 0.536763 3.2:+82669346 MS.gene055489:CDS
ATTGCTCTTCAAAATGACAC+TGG 0.537117 3.2:-82668181 None:intergenic
AGTAATCCTTCAATAAGCTA+GGG 0.541163 3.2:-82664530 None:intergenic
TGTTTGAGTATAGGAATACT+GGG 0.542687 3.2:+82668262 MS.gene055489:CDS
CAAGGATTATGTCTCATACA+TGG 0.543266 3.2:+82664321 MS.gene055489:CDS
GCATGCATTGCACTAAATGC+CGG 0.547137 3.2:+82666781 MS.gene055489:CDS
CTGTAAATAGGTTTATCTAG+TGG 0.547357 3.2:+82667886 MS.gene055489:intron
TAAGCTGCTAAATATAAATG+AGG 0.548356 3.2:+82669175 MS.gene055489:CDS
GGAACATAAATTGTCTCTCC+AGG 0.550858 3.2:-82666171 None:intergenic
AGGAAGCCTTTCGTCATCTG+TGG 0.551264 3.2:-82666857 None:intergenic
AAACTAACTGATCCGTCCGC+CGG 0.551543 3.2:-82668288 None:intergenic
GATCGCAGAATAGACGCTCT+TGG 0.552054 3.2:+82662189 MS.gene055489:CDS
ATCCAGCTCTTACAAGTGCA+TGG 0.557351 3.2:+82664667 MS.gene055489:CDS
ACCACGTTGGAGTGAAATCC+TGG 0.558962 3.2:+82669444 MS.gene055489:CDS
GACGGATCAGTTAGTTTAGC+CGG 0.560179 3.2:+82668294 MS.gene055489:CDS
GCGACGCGAGCAGCATCTTG+AGG 0.560780 3.2:-82662267 None:intergenic
GGATTGCTACCCGAACCAAC+AGG 0.563415 3.2:-82666877 None:intergenic
GTGGGAAGATACAACAAACT+TGG 0.564350 3.2:+82668517 MS.gene055489:CDS
GAGGTAGAGCAAATCGATGA+TGG 0.564450 3.2:-82669371 None:intergenic
TTGCATTTAAAATTTCTCAA+AGG 0.564534 3.2:+82664272 MS.gene055489:CDS
CGTGGATGGTTTCCCGTTTG+GGG 0.564553 3.2:+82668224 MS.gene055489:CDS
TTGTTACCATCAAAATGTAA+AGG 0.567945 3.2:-82663145 None:intergenic
AGAAGCTATAATTTATGGAA+AGG 0.568532 3.2:+82666495 MS.gene055489:CDS
TGAGACTCCATCATCACTGC+AGG 0.568853 3.2:+82665950 MS.gene055489:CDS
CATAAAGGAGTCTGCCGTGT+GGG 0.569358 3.2:+82666309 MS.gene055489:CDS
CCAGAATCTTAACAAAGAAG+AGG 0.570253 3.2:+82668708 MS.gene055489:CDS
AGCCTTTCGTCATCTGTGGG+AGG 0.570411 3.2:-82666853 None:intergenic
AAACGGGAACTAGTGGGGAA+TGG 0.574590 3.2:+82670703 MS.gene055489:intron
ACCCCACTGGCCATAACACT+AGG 0.575861 3.2:-82668102 None:intergenic
TCAGGTCATGCTAAATGGAC+TGG 0.577021 3.2:+82664186 MS.gene055489:intron
AGCATCTTCTGTATTCCCAC+TGG 0.577196 3.2:-82669393 None:intergenic
AGCTATAATTTATGGAAAGG+TGG 0.579530 3.2:+82666498 MS.gene055489:CDS
ATTCCTAGTGTTATGGCCAG+TGG 0.580711 3.2:+82668099 MS.gene055489:CDS
TCTCAAGCCGTTTGACCAAG+TGG 0.584548 3.2:+82668738 MS.gene055489:CDS
CTTTCGTCATCTGTGGGAGG+GGG 0.587951 3.2:-82666850 None:intergenic
TCCTAGTGTTATGGCCAGTG+GGG 0.588471 3.2:+82668101 MS.gene055489:CDS
AATACTGGGATTCCGGCGGA+CGG 0.589838 3.2:+82668276 MS.gene055489:CDS
GTCAACGGGAACTAAGAGAA+TGG 0.591171 3.2:+82666610 MS.gene055489:CDS
AGCAGCTTAGATAAATCAGA+TGG 0.592390 3.2:-82669161 None:intergenic
TTTGATAATTAAGCGATGTG+AGG 0.594501 3.2:+82668338 MS.gene055489:CDS
TCGATTTGCTCTACCTCCAG+TGG 0.595230 3.2:+82669377 MS.gene055489:CDS
ACAGAGAAATGCAACGTGGA+TGG 0.595885 3.2:+82668210 MS.gene055489:CDS
TATTGGACTGTGGAGAGTTG+AGG 0.598470 3.2:+82670741 MS.gene055489:CDS
GTAAATAGGTTTATCTAGTG+GGG 0.600129 3.2:+82667888 MS.gene055489:intron
ATCTTCTGTATTCCCACTGG+AGG 0.600340 3.2:-82669390 None:intergenic
TATGAGAGATCTAATATGGA+AGG 0.601472 3.2:+82666662 MS.gene055489:CDS
TGACTTCAGAATTTCTCAAG+TGG 0.604991 3.2:+82669287 MS.gene055489:intron
AATCTTAACAAAGAAGAGGA+AGG 0.605956 3.2:+82668712 MS.gene055489:CDS
AATACAGAAGATGCTGCCGA+GGG 0.606356 3.2:+82669401 MS.gene055489:CDS
GTATGTTTGGATATGGCACC+TGG 0.607998 3.2:+82666279 MS.gene055489:CDS
ATTATAAGCCACCTATAGGA+TGG 0.608178 3.2:-82664609 None:intergenic
CCCCTCCCACAGATGACGAA+AGG 0.610507 3.2:+82666851 MS.gene055489:CDS
TTGCACTAAATGCCGGCAAG+TGG 0.611811 3.2:+82666788 MS.gene055489:CDS
GTGCATGTTAATGAAGAAGA+TGG 0.611852 3.2:+82665915 MS.gene055489:CDS
AGCTCCACTCAAAATTGTAG+TGG 0.612842 3.2:-82666572 None:intergenic
GGAAGCCTTTCGTCATCTGT+GGG 0.613871 3.2:-82666856 None:intergenic
CAGAAGAGACAGACTATCGT+GGG 0.616345 3.2:+82668499 MS.gene055489:intron
CAAACTTGGCCTCCTTCCTG+GGG 0.618358 3.2:+82668531 MS.gene055489:CDS
TGTTTAGAAAGAGAAATAAG+TGG 0.619138 3.2:+82666465 MS.gene055489:CDS
TTGCTCTTCAAAATGACACT+GGG 0.620536 3.2:-82668180 None:intergenic
GAATACAGAAGATGCTGCCG+AGG 0.621862 3.2:+82669400 MS.gene055489:CDS
TACAGAAGATGCTGCCGAGG+GGG 0.626315 3.2:+82669403 MS.gene055489:CDS
TTGTCTTCGTCAGCAAGAAG+AGG 0.627512 3.2:-82666126 None:intergenic
AGGAATATGTTTCCTGAGAG+GGG 0.628568 3.2:-82668082 None:intergenic
GTGGATGTGGTAAAAGATGA+AGG 0.628693 3.2:-82668568 None:intergenic
CGATTTGCTCTACCTCCAGT+GGG 0.629855 3.2:+82669378 MS.gene055489:CDS
CAGATGAAACGTAAATCTTG+AGG 0.630314 3.2:-82670778 None:intergenic
ATACAGTGCCACCATCCACT+CGG 0.631096 3.2:-82666192 None:intergenic
TCAAACAATGCAGCAGAATG+GGG 0.631106 3.2:+82668677 MS.gene055489:CDS
GACGTTCGAGAATTGAGCAG+AGG 0.634922 3.2:-82662239 None:intergenic
GATGACGAAAGGCTTCCTGT+TGG 0.636057 3.2:+82666862 MS.gene055489:CDS
TTTATGTTCCGAGTGGATGG+TGG 0.637501 3.2:+82666184 MS.gene055489:CDS
TTAAACTGGTCTGAGGTGAA+AGG 0.638216 3.2:+82668629 MS.gene055489:CDS
TTCAAGATAAACAACCCCAC+TGG 0.638268 3.2:-82668115 None:intergenic
ATTGAATGTACACAACTACC+TGG 0.639305 3.2:+82666153 MS.gene055489:CDS
GCAGAAGAGACAGACTATCG+TGG 0.640708 3.2:+82668498 MS.gene055489:intron
CTAGGAATATGTTTCCTGAG+AGG 0.644586 3.2:-82668084 None:intergenic
ACTCCTTTATGACGATAACC+AGG 0.653563 3.2:-82666297 None:intergenic
CAAGAGCAAGCAAACCCACA+CGG 0.656542 3.2:-82666323 None:intergenic
GAGACAATTTATGTTCCGAG+TGG 0.659270 3.2:+82666177 MS.gene055489:CDS
TCAGTGTGTATCCAAGAGCA+GGG 0.663517 3.2:-82669320 None:intergenic
AAGGAGGTAACTAGGACACC+AGG 0.663763 3.2:-82669462 None:intergenic
GTAATCCTTCAATAAGCTAG+GGG 0.667445 3.2:-82664529 None:intergenic
AAAAGTGCCTTTAGGTGTGA+CGG 0.668167 3.2:+82665893 MS.gene055489:CDS
GGTTTATTGAGTGACCACGT+TGG 0.675486 3.2:+82669431 MS.gene055489:CDS
AGCTGGATCAACATGCCAAG+AGG 0.677057 3.2:-82664652 None:intergenic
TGTATAAAATGTATCCAGTG+TGG 0.684015 3.2:-82663328 None:intergenic
TATCCAGTGTGGTATGACAC+CGG 0.684470 3.2:-82663317 None:intergenic
ATAAACGACCATCCTATAGG+TGG 0.685021 3.2:+82664601 MS.gene055489:CDS
AAACTTGGCCTCCTTCCTGG+GGG 0.689807 3.2:+82668532 MS.gene055489:CDS
TGATAATTAAGCGATGTGAG+GGG 0.689937 3.2:+82668340 MS.gene055489:CDS
ATGACTCTATGCCTAAAAGG+AGG 0.692833 3.2:+82662929 MS.gene055489:CDS
TTATACAGAGGGTGGCAATG+TGG 0.697663 3.2:+82663344 MS.gene055489:CDS
TCATAAAGGAGTCTGCCGTG+TGG 0.699636 3.2:+82666308 MS.gene055489:CDS
AGATTGGACATATTGGACTG+TGG 0.706385 3.2:+82670731 MS.gene055489:CDS
ATACCGGTGTCATACCACAC+TGG 0.707579 3.2:+82663314 MS.gene055489:CDS
CTGTATCTAAGATATCGAAG+AGG 0.711016 3.2:-82664578 None:intergenic
TAGGAATACTGGGATTCCGG+CGG 0.711408 3.2:+82668272 MS.gene055489:CDS
ACGTGAATTAAACTGGTCTG+AGG 0.711510 3.2:+82668622 MS.gene055489:CDS
GCTTTATGGTTTAGGCACAG+AGG 0.719101 3.2:+82667955 MS.gene055489:CDS
ATACAGAAGATGCTGCCGAG+GGG 0.739771 3.2:+82669402 MS.gene055489:CDS
CAAGAGGCACCAGATCTCTG+TGG 0.740954 3.2:-82664636 None:intergenic
CATCACAGAGAAATGCAACG+TGG 0.742244 3.2:+82668206 MS.gene055489:CDS
AAAGATCACTGAAATCAAGG+AGG 0.742693 3.2:-82669478 None:intergenic
ACCAGGATTTCACTCCAACG+TGG 0.759662 3.2:-82669445 None:intergenic
GATGTGGTAAAAGATGAAGG+TGG 0.808325 3.2:-82668565 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.2 gene 82662153 82670825 82662153 ID=MS.gene055489
chr3.2 mRNA 82662153 82670825 82662153 ID=MS.gene055489.t1;Parent=MS.gene055489
chr3.2 exon 82662153 82662301 82662153 ID=MS.gene055489.t1.exon1;Parent=MS.gene055489.t1
chr3.2 CDS 82662153 82662301 82662153 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82662892 82663005 82662892 ID=MS.gene055489.t1.exon2;Parent=MS.gene055489.t1
chr3.2 CDS 82662892 82663005 82662892 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82663102 82663160 82663102 ID=MS.gene055489.t1.exon3;Parent=MS.gene055489.t1
chr3.2 CDS 82663102 82663160 82663102 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82663283 82663425 82663283 ID=MS.gene055489.t1.exon4;Parent=MS.gene055489.t1
chr3.2 CDS 82663283 82663425 82663283 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82664190 82664387 82664190 ID=MS.gene055489.t1.exon5;Parent=MS.gene055489.t1
chr3.2 CDS 82664190 82664387 82664190 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82664516 82664715 82664516 ID=MS.gene055489.t1.exon6;Parent=MS.gene055489.t1
chr3.2 CDS 82664516 82664715 82664516 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82665872 82665971 82665872 ID=MS.gene055489.t1.exon7;Parent=MS.gene055489.t1
chr3.2 CDS 82665872 82665971 82665872 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82666108 82666683 82666108 ID=MS.gene055489.t1.exon8;Parent=MS.gene055489.t1
chr3.2 CDS 82666108 82666683 82666108 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82666778 82666900 82666778 ID=MS.gene055489.t1.exon9;Parent=MS.gene055489.t1
chr3.2 CDS 82666778 82666900 82666778 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82667896 82667976 82667896 ID=MS.gene055489.t1.exon10;Parent=MS.gene055489.t1
chr3.2 CDS 82667896 82667976 82667896 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82668048 82668379 82668048 ID=MS.gene055489.t1.exon11;Parent=MS.gene055489.t1
chr3.2 CDS 82668048 82668379 82668048 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82668502 82668759 82668502 ID=MS.gene055489.t1.exon12;Parent=MS.gene055489.t1
chr3.2 CDS 82668502 82668759 82668502 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82669112 82669196 82669112 ID=MS.gene055489.t1.exon13;Parent=MS.gene055489.t1
chr3.2 CDS 82669112 82669196 82669112 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82669296 82669506 82669296 ID=MS.gene055489.t1.exon14;Parent=MS.gene055489.t1
chr3.2 CDS 82669296 82669506 82669296 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
chr3.2 exon 82670716 82670825 82670716 ID=MS.gene055489.t1.exon15;Parent=MS.gene055489.t1
chr3.2 CDS 82670716 82670825 82670716 ID=cds.MS.gene055489.t1;Parent=MS.gene055489.t1
Gene Sequence

>MS.gene055489

ATGGATTCTCAACAAGCTCACACTCACATTCAGATAGATCGCAGAATAGACGCTCTTGGCGATCTTCGAGTTCTTCCCGATGAAATCCTCTGCTCAATTCTCGAACGTCTCACTCCTCAAGATGCTGCTCGCGTCGCTTGCGTTAGCAGGTAATTCCTTCATCGTTTGCTTTCATTTTTCCATATCGATTTGATAAGGAATCTGGTTGCACTGTTTCATTCAAGTACACTTTGGCGTTTGTTTTGATTCATCACATTAATCAACGGAACCTGTTTATGGCAATAGTATAAATGATTCCGTTTGAATTTTGAATTTCTTATACGAGCGTCATTAATGATTGATTACGATCCTGTTAGGATAAATAGCTTTATTTGCAACTTATAGCATTAACACTTATCACGATAAGAGCCTATGTATAAACTATTTCTATTAAAAAAGATAGCACAAGGTTAAATTATTTTCGTATAAGCTATAGTTTGTTTTTATAAGCTATCTTGGATAACTTATGAGAATAACCTGAAAACAGCAGCTTATGAACATGTCATAAGCTGTTTTCATAAGTTTTTCTAAGCAGTCTTACAAGTGCTTATGCGTTAGGTAAGCTCAAATAAGTCAATCTAAACAGGCTCTTAGAAACTTTTCTTTAGATATTATAATTTTATTAACCGCCACATAGCTCTTGAGCACTTTAGCTGTTATAAATGACGGTTAATTTTTTTCTTCATTTATTTATTGACAGTGTGATGTATATACTATCCAATGAAGAACCACTATGGATGACTCTATGCCTAAAAGGAGGAGCATCTGGTTTTCTTCAATACAAAGGCTCTTGGAAGAAAACTGCTCTGCATAAGTACGATGTCGCTCACTCTCTCAATGTACAATTGCCTGTTTTATTCATTTTTATATTTAATGCTAACATATTTGGAATCGGATATTTTGAATGCAGTTTGAATCTTCCAGAGAAGTACAAAGAGTGCCATAGACAGCAGCCTTTACATTTTGATGGTAACAATTCTGATATCCTAAGAATCTGATCTGTCCTATATTTTTTCAGGAAACTTTTTGCTGTATGGAGTTAAAAAGTCATATGGTTTTTGAATATACAACTGATGCTAACCTTCCTGCAGGATTCAACTCTTTGTTTTTGTATCGGAGATTATACCGGTGTCATACCACACTGGATACATTTTATACAGAGGGTGGCAATGTGGAAAGAATAAATGACATCTCAATGAAGGACTTCAACGACAAGTATGACATGAAGAAACCGGTACAGAGATTTAAAGAATTTAGTAGTAAACCCACATAAATTTTTCCCCTCGTGTTGGTTCTGTTTCAGTGTCAACCATTAATTTTGAGCTGTCTTCCTGGCGAAAAGAAAATATCCTGAGCTCTATTTTCCATTGTTTATTTTGCTGGAATTTTTGTGAAAGAAATGAATGCTGTGGCAATACACATTTTTTTGTGATACAAAATACTCCCTCCGGTCCTATTTATAAGAGAAAATTGGGTCAACAAAACTTGATGTATCTGGACTGAATTTTGAACCAGATACATCAACTTTTGTTGACCCAATTTTCTCTTATAAATAGGACCGGAGGTAGTATCTCTTTTTTAAAAATAAATAATACACTTAAAAAATGACCAGTTTACGAGGCCAGTGTGTTGACTTTAAAACGTGGACGATAATGAAGATTGTGTTCAAAAGCATGTGTTGATAGTATTTCTCTTTTGAAAAAGAGGAATCATTGTTATTTATTTTTTCTCATTATATATATGTAGTTGTGTACATATTTTGCTCTTGATCTCTGAAGCATTTGGGTAGCTGTAGTGTTTTTTTCCCCAGCTTTTTCTTGTAAAGATGGAAAATGAGAGACGAGAATTAATTATTTTTATAACTACAACCGTCCTTTGTGTAGTAGGGTTATAGTTGATTTTGCATTTTAGATATCTTGGAATTTATCAATTTTGAACCATCAAACATTGGTGGTTTTCTTTTTGTTGAAATGTAATTTTGATGAATCACACCCTCAGGTCATGCTAAATGGACTGGCGAATACATGGCCTGCCAGGCAGAAATGGACAACTGATCAGCTGTTACAAAACTATGGAGATGTTGCATTTAAAATTTCTCAAAGGAGTTCCAAAAAGATCTCCATGAAGTTCAAGGATTATGTCTCATACATGGAAGTTCAACACGATGAAGACCCATTATATATTTTCGACGAAAAGGTGCTTGCGTTGAGATATTCCTGTTTCAATAATGCTCTTCCCTGTCTCCCCACACTACTCCTTTCAGTTTTTCTTGTTTTCAAATTTGAGATTTTGGAAGTTCATTTAACTTTTAATTTAACCAACAGTTTGGCGAACATGCCCCTAGCTTATTGAAGGATTACTGCGTGCCTCATCTTTTTCAAGAAGACCTCTTCGATATCTTAGATACAGATAAACGACCATCCTATAGGTGGCTTATAATTGGACCACAGAGATCTGGTGCCTCTTGGCATGTTGATCCAGCTCTTACAAGTGCATGGAATACTCTTCTTTCTGGCCGTAAAAGGTAAAAAAGATGCTCAACATGAATTTTTTATTTGCAATATGGTTTTGAATCATGTAGCTTGGACCATGACTGTTTACATGGTATCTGAGGAAACTACGTGCTTCCTTGTTTTTAGTTTAAGCCTTAAACATAATTAGTTGGCTACTCGGTTCTTCTAGCTTATTGCTGCTCTTTTCTTTTTCTAGTTAAAATTAGGCTCCCAAAATTTAATGAAGGGGATAGGGAGAGGTGGATAAAATTTGAGCATTGAGACTCACTAGGGATCTAACTGGGCATCAGTTTTTTTTTCCCCATTGGGAAGCTGGGTGCGTTTAGGCTGCAAGTCAATTGGCTCGTCCATGCAAATATGTTGTTCTGTTTACGAGTTTCACTTTTGATTTTGACTGCTAATGCAACGTAAACTAGCTCACCATTTTATGCTTTTAATGATTCAAAGCAAAGTTTTCTCTGGAATTTGTAAGATTGGAATAGTTTTTGCAAAGTAAGATTAGCCACTTTTAGACTGAGAGTGCTAATATCTTGTTAGGACTAGTTTCGTAAAAAAATTTATTGACAAATGTTAAAAAGATTCCTAAAACAATGACTCTTGAATCCTCTGTAGACATTTAACTATTTTCAGGCTTCTGCGTCTTAGATGGGAGTAGATAGGAGTTGGAAATAAAAATGCACAAAGAATAAGAAGTCGCCAAGTATTATCAAACTCAACTAGATACAAACAAGTATGCTTAATTATGCAGTAGTAGTACGGAGTAGTGGCTACAGTTGAACAGAAAATAATGTACCACCCGTGAATAGGCCGGGAGGTTATCCTTCACTCTTGTCATGTTAAAAATTATTGTCCAAATCAACACATTCTATTAGCAATTTATTGAAACATTAACACTAGGATAGGACCAGGTTTGCTTAATTGGAAAAATGAGTGCACTTGATTTAAGAAAGTGAAACTGCATGGACCAAAATTAACGCATTCTTAAAACTACAATGGCCAGAAGCGTATTTAAATATTTAACTTAAATTAGAGTTCTTGTTCTTTAATGATTCTACAACTAATAACTTAAATTTTATGTAATTTGGTGCATGGATTTTCTGTGTTGGTGCTTGATTATTTTCCAAATAATTAATGCTCAAGAGGTATGTCTTGCTTAATATTTGCTAGGTGGGCATTATATCCTCCTGGAAAAGTGCCTTTAGGTGTGACGGTGCATGTTAATGAAGAAGATGGTGATGTCAGTATTGAGACTCCATCATCACTGCAGGTTCTAGAATCTTGCAATTTGAGCAATCTACATATCCTTTTTTTGGGTTTAAGTAATTATTGCCATTAGGATGTCAACTACATTGTGTCTATCTGTTGAATTTTTTATTATTAATTACATTTATGTTTGCTTACAGTGGTGGTTAGATTTTTATCCTCTTCTTGCTGACGAAGACAAGCCAATTGAATGTACACAACTACCTGGAGAGACAATTTATGTTCCGAGTGGATGGTGGCACTGTATTTTAAATTTGGAAACAACTATTGCAGTCACGCAGAATTTTGTGAATTCTAATAACTTCGAATTTGTATGTTTGGATATGGCACCTGGTTATCGTCATAAAGGAGTCTGCCGTGTGGGTTTGCTTGCTCTTGAAGAAGATGTTTATGAAAATGTCATACAGAACATGTCATGTAATGAAGAAAACTTAAGTTACTCTGATTTGTCAAGGAAAGAGAAAAGATCCAAAACTCTGAAAGATGTAGACGATCTATGTTTAGAAAGAGAAATAAGTGGTGCATCCAGAAGCTATAATTTATGGAAAGGTGGATTTTCTTATGATATAAATTTCTTATCCATGTTTTTGGATAAGGATAGAGACCACTACAATTTTGAGTGGAGCTCGGGCAACAGCATTGGTCAACGGGAACTAAGAGAATGGTTGTCCAAGCTTTGGATTCAAAAACCAGATATGAGAGATCTAATATGGAAGGTAATTTTGTTTCTAAAGTAATGCATTCTTTTTGTGCTCACAAAGTAAGTATATTATCTGATTTTATGACTTTTTTCTGTTTATACATGTCCAGGGAGCATGCATTGCACTAAATGCCGGCAAGTGGTTAGAATGCCTGTCAAAAATTTGTGCCTTCAACAACTTGCCCCCTCCCACAGATGACGAAAGGCTTCCTGTTGGTTCGGGTAGCAATCCTGTGAGTTTTTATTTATGTAATTAATAATTTTTACTGTTTGCATTTTTTGAGAAGCTTGCAGTACATCGAAATTTCAAATGCTTCATAAAAAATAATTGGTCATAGGTGTCCCAAACAATATTGATGACCGCTTCAAAATTAGAATATAATTCAGTGGATCATTTAGAAAATTAGAAGGATTTTCTGGTTGAATCAAAGTCGCGATGTTTTGCTTAAAGTCTTTCCCTTGTAAGAAACAAATTCTCATCCTCAACATGTCCTCCCTTACTGTACTTTATCAATATTTTGGACCTGTATTTGGAGACTAATTACAACAGTCTTAGCATCTAAAAACTAGTTTTCATTTCAAGATACCTTTCATATTTGCATCGAGACTAGAACAGGTTACCTTTGTAGGAAGGCACTTTGGTCACCGAAGTTTGGTGCACATGGTTCGATCCCTATAGGCTCCAACTTTTTAAAATTCATGATTTTTCTACAAAACCGTGCATTTAATGTTATACCGGATTGACTCGAAAACCGACCGAGTTACCGGTTATGACCGGTTTTCAGCGAGTCTCACCGGTTCACACTGGTCTAATTACATGACCGATTCATAAGGTGGCTCGACTCGGTCTAATCACCGGTTCACGGTCGGACCGGTTCAACCGGCCGGTTCGAGCCGAGTTTTAAAACTATGCTTTGGTGGAACTGACCTGCATACTATCTTTATGCCTTTGTAATATTAATCATACAAAGGAGTCGGTTGCAGCAATTAGTAAAACATGATGGCGTGTTTTTAAAATTTCTCTTCAAACAGTCGGATTCATTCTGAAATTATCTCGTTGGTGCTTACCATAACTTGGCATGATATGCTATCTAATTACTTAGCCATGTTTCTTATGATTCTGCTTTTGAATTCTGACATGAGACCGGTTTTTGGTATAGTCATTCATTATCTTGTAGAGTACTTCTATAAAGTCTCTAACTATGAACATTTTCCATTCTGTAAATAGGTTTATCTAGTGGGGAACTATGTTGTTAAGATTTTTGTCGAAGAAGGACTGGAAGCATCGCTTTATGGTTTAGGCACAGAGGTACTTGATTCATATTCTATTTTCATTCCATCTTCTGTACATATCTAACTTTCATATTGTATCTCTTACAGTTAGAGTTTTACAGTCTGCTGCTTGAAGCCAACTCCCCTCTCAGGAAACATATTCCTAGTGTTATGGCCAGTGGGGTTGTTTATCTTGAAGACGAATCCTATACCAATTTAAGTTGGGATGGAAAAGGAGTTCCCAGTGTCATTTTGAAGAGCAATATCATCACAGAGAAATGCAACGTGGATGGTTTCCCGTTTGGGGTATGGGGCAAGCAATTGTTTGAGTATAGGAATACTGGGATTCCGGCGGACGGATCAGTTAGTTTAGCCGGTAACTCAAGTATATGGCCATATTTGATAATTAAGCGATGTGAGGGGAATATGTTTGCAGACTTGTAAGTGTTGTCCTTGTGGTTTATAAATAATTTCATCATGCAGCATAGCAATTCAAATTTCCTACTTCTGCAGGCCACACAACTCTCTTAATATTGTAGAGTTTGTTTTCATTATTTTGCAGAAGAGACAGACTATCGTGGGAAGATACAACAAACTTGGCCTCCTTCCTGGGGGAGCAAATGCGCCACCTTCATCTTTTACCACATCCACCTTTAAATAATTCATTTATATCTGATATTGAACGTGAATTAAACTGGTCTGAGGTGAAAGGTTGCACTGCAAATGTTAATTGTAAATCAAACAATGCAGCAGAATGGGGACTCTTCACCAGAATCTTAACAAAGAAGAGGAAGGACGTCTCAAGCCGTTTGACCAAGTGGTATACTTAGTTTTATTGAGCTCTCCAATTTCTTGATAAACAGGATAGTTCAAGAATGAGAGAGGGGTTATCATGCATACAATTTAAAATCTAATGATCTTCTGAGCATTTTAAGTTTAAAGTTTCAAAGTTTTATGGCTTTTCCTGATTAAAGGAAGTGCTACTTTTGGTAGCTGTGTTTTCCAATTGTTTATAAACTCTCTTTTGACTTTGGCTCACTTAAGATTATATTTTATCCATGCATGATAAATGTATTCCATTTTTGGGTTTGCCTTGGTGCTTCTGTTGCATGCTAGCTTCACATTTTTATTTGTATGGCGGGCCTCTGATGCTTCGTACTAAAACTTTGCAGGGGAGATCCAATTCCTAGCAAACTGATTGAGAAAATCGATGAATATCTACCATCTGATTTATCTAAGCTGCTAAATATAAATGAGGTTTGTTCTAAATTCACTATTTCATCCCAACATAATTTTTTTTTTTCATTGGATATGCTACATTAAGTTTGTTTTTTGTCAACTTCTATTTGACTTCAGAATTTCTCAAGTGGCACTTCTAAACCCTGCTCTTGGATACACACTGACATTATGGATGATAATATTTACATGGAACCATCATCGATTTGCTCTACCTCCAGTGGGAATACAGAAGATGCTGCCGAGGGGGACAATGGTTTATTGAGTGACCACGTTGGAGTGAAATCCTGGTGTCCTAGTTACCTCCTTGATTTCAGTGATCTTTCTATGGGTCAGTACCTTTTCTTTTCTACCTGTTCCATAAATTTACATCCAACTTCAGTCATACCCGTCTTTATTAGAAATGGCTTACATTGGAATGTTGTCATGATGTGGTATCATAATTTATCAAGGGGCCTTTCAGTATCTGCAAGATTATTTTATTAATCTGTAGAGTTGAGCTGTGTTTTCTTTAAAGAAGAGCAAGGCTTGTGTTATTGACCCCTACATGTAGGGTTTAATTTTCCTATGATCGTCACTTCCTTTCTTCCTCCACCATTTTTATGATTTTCAGCCAAATGATGTTCTGCATGTAATTGTAATATTGCAGTTTCATTCTTAACAATTCACTTGCTTAATTTTTGTTATGAAGTCATTGTTGGCCTTGAGATGCATATTGACTGCAGACCACTGATTAAGAAAGTGTATGTGAGAACCCACTTGGATTGGGCTGGTGGTATTGGCTTGGGATCTGGGAGTGTGTCCCAGGTTCGATTCTCCCCAATGTCAATTTGGGTGGGCTAATTTAGCTTCTTCAAAAAAAAAAAGTGTATGTGAGTAGAAATAAAAAGGGTATTGTAAGTAATAACTACCTCAAGAATACAAATTTAGTTAGTTATAAGAAAATGCCATAAATAGGATAGAATACAAATTTAGTTTGTTATAAGAAAATTTTATAAATAGGATAAAAGGGAATTTGTGGGGACAGGTGTTAAAATCGGAAATAACGGAGAATTGAGTAGCTCTCAAATTCCTTAAGACTGTGCTTTTGCGTGTCTGTGTCACATTGATCAAGATCTATCAATAAATCACTGTTTTCTCTCTGTGAATTCTCTCTTTTATGCTCCAGTTCTCAACTGGTAATAGTTCATGAAAAGACAGAAATCAGAGAAATATTTTGGGAAAAATGTAACCACTAATTGACTTCAAAAACTACTGGTTCATTATTGTTTTGGGAAAAATGTTTCTGGTTTAGGAATCATCATTAACCCTTTGTTTGGCTATGATGTTTAAAGAGTGTTTGATGAGTAGCCACTAATTGACTTCAAAAACTACTGGTTCAGTATTCCCAAGTTTGCCAATGATATTTGATAGTAGACCACTCATTAAGAGAGTGTATGTGAGGAGAAATAAAAAGGGTATTGTAAATAATAGCTAGCTAACAGGGTACAAATTCAGTTATTAGTTATAAGATGTTATAAATTGGTGAAACGGGAACTAGTGGGGAATGGTGTTGAGATTGGACATATTGGACTGTGGAGAGTTGAGGAACTCTGGATTCAGCCTCAAGATTTACGTTTCATCTGTGTCACATTGACCAAGACCCATTAA
Protein sequence

>MS.gene055489.t1

MDSQQAHTHIQIDRRIDALGDLRVLPDEILCSILERLTPQDAARVACVSSVMYILSNEEPLWMTLCLKGGASGFLQYKGSWKKTALHNLNLPEKYKECHRQQPLHFDGFNSLFLYRRLYRCHTTLDTFYTEGGNVERINDISMKDFNDKYDMKKPVMLNGLANTWPARQKWTTDQLLQNYGDVAFKISQRSSKKISMKFKDYVSYMEVQHDEDPLYIFDEKFGEHAPSLLKDYCVPHLFQEDLFDILDTDKRPSYRWLIIGPQRSGASWHVDPALTSAWNTLLSGRKRWALYPPGKVPLGVTVHVNEEDGDVSIETPSSLQWWLDFYPLLADEDKPIECTQLPGETIYVPSGWWHCILNLETTIAVTQNFVNSNNFEFVCLDMAPGYRHKGVCRVGLLALEEDVYENVIQNMSCNEENLSYSDLSRKEKRSKTLKDVDDLCLEREISGASRSYNLWKGGFSYDINFLSMFLDKDRDHYNFEWSSGNSIGQRELREWLSKLWIQKPDMRDLIWKGACIALNAGKWLECLSKICAFNNLPPPTDDERLPVGSGSNPVYLVGNYVVKIFVEEGLEASLYGLGTELEFYSLLLEANSPLRKHIPSVMASGVVYLEDESYTNLSWDGKGVPSVILKSNIITEKCNVDGFPFGVWGKQLFEYRNTGIPADGSVSLAGNSSIWPYLIIKRCEGNMFADLRDRLSWEDTTNLASFLGEQMRHLHLLPHPPLNNSFISDIERELNWSEVKGCTANVNCKSNNAAEWGLFTRILTKKRKDVSSRLTKWGDPIPSKLIEKIDEYLPSDLSKLLNINENFSSGTSKPCSWIHTDIMDDNIYMEPSSICSTSSGNTEDAAEGDNGLLSDHVGVKSWCPSYLLDFSDLSMVGNGVEIGHIGLWRVEELWIQPQDLRFICVTLTKTH