Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055663.t1 | XP_013468354.1 | 94.6 | 56 | 3 | 0 | 39 | 94 | 27 | 82 | 3.60E-20 | 107.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055663.t1 | A0A072VLC9 | 94.6 | 56 | 3 | 0 | 39 | 94 | 27 | 82 | 2.6e-20 | 107.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene055663.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055663.t1 | MTR_1g069020 | 92.982 | 57 | 4 | 0 | 39 | 95 | 27 | 83 | 1.24e-30 | 113 |
| MS.gene055663.t1 | MTR_1g069015 | 83.929 | 56 | 9 | 0 | 39 | 94 | 23 | 78 | 2.48e-25 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGACAGAAGCTAACATTCC+TGG | 0.419560 | 1.4:-47940364 | None:intergenic |
| ATTCAGGGAAGAAGCGGTTC+GGG | 0.436156 | 1.4:+47941120 | MS.gene055663:intron |
| GATTCAGGGAAGAAGCGGTT+CGG | 0.441206 | 1.4:+47941119 | MS.gene055663:intron |
| CTTAGGTTACATTATACCAT+TGG | 0.470347 | 1.4:+47940705 | MS.gene055663:intron |
| AGGTTACATTATACCATTGG+TGG | 0.498266 | 1.4:+47940708 | MS.gene055663:intron |
| TTCCTCACAATTGAAAAGCC+AGG | 0.501686 | 1.4:+47940346 | MS.gene055663:CDS |
| AGATGCCGGAGATGGCTAAA+CGG | 0.516595 | 1.4:-47940760 | None:intergenic |
| TTACTTTGTGGAAGAGATGC+CGG | 0.520785 | 1.4:-47940774 | None:intergenic |
| TGTGGAAGAGATGCCGGAGA+TGG | 0.553990 | 1.4:-47940768 | None:intergenic |
| TATCTCTTGTGAACAAGAAG+TGG | 0.555948 | 1.4:-47940287 | None:intergenic |
| CTCTTGTGAACAAGAAGTGG+AGG | 0.556696 | 1.4:-47940284 | None:intergenic |
| AATCACCGTTTAGCCATCTC+CGG | 0.559295 | 1.4:+47940755 | MS.gene055663:CDS |
| TCTTGTGAACAAGAAGTGGA+GGG | 0.597209 | 1.4:-47940283 | None:intergenic |
| ATGATTTAACGAGCCACCAA+TGG | 0.597436 | 1.4:-47940721 | None:intergenic |
| GTTTCCCTTGAGACTGTCGA+TGG | 0.607459 | 1.4:+47941181 | MS.gene055663:CDS |
| ATATACCATCGACAGTCTCA+AGG | 0.611734 | 1.4:-47941186 | None:intergenic |
| GTTAGCTTCTGTCAGACGCA+AGG | 0.614739 | 1.4:+47940372 | MS.gene055663:CDS |
| GTTGTGATTCAGGGAAGAAG+CGG | 0.620242 | 1.4:+47941114 | MS.gene055663:intron |
| TATTATCAAAAGATATGATG+AGG | 0.626070 | 1.4:+47941159 | MS.gene055663:CDS |
| TATACCATCGACAGTCTCAA+GGG | 0.636705 | 1.4:-47941185 | None:intergenic |
| TGGCTCGTTAAATCATCTCA+AGG | 0.637214 | 1.4:+47940728 | MS.gene055663:CDS |
| CTTGTGAACAAGAAGTGGAG+GGG | 0.638845 | 1.4:-47940282 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATACAAAAAATAAATTAAA+AGG | - | chr1.4:47940987-47941006 | None:intergenic | 10.0% |
| !! | TATAATGTAACCTAAGAAAA+AGG | - | chr1.4:47940701-47940720 | None:intergenic | 20.0% |
| !! | TATTATCAAAAGATATGATG+AGG | + | chr1.4:47941159-47941178 | MS.gene055663:CDS | 20.0% |
| !!! | TCATCATATCTTTTGATAAT+AGG | - | chr1.4:47941160-47941179 | None:intergenic | 20.0% |
| ! | AATCTTGTTGAATCAGAAAT+TGG | + | chr1.4:47940850-47940869 | MS.gene055663:intron | 25.0% |
| ! | ACATTTAATGTTGTGATTCA+GGG | + | chr1.4:47941105-47941124 | MS.gene055663:intron | 25.0% |
| ! | TTCTGATTCAACAAGATTAT+TGG | - | chr1.4:47940848-47940867 | None:intergenic | 25.0% |
| !!! | AAAATCTTTGGAAAGTTTTC+AGG | - | chr1.4:47941032-47941051 | None:intergenic | 25.0% |
| !!! | ATATCTTTTGATAATAGGAG+CGG | - | chr1.4:47941155-47941174 | None:intergenic | 25.0% |
| AAACACGAGTTTAATGTGTT+TGG | - | chr1.4:47940441-47940460 | None:intergenic | 30.0% | |
| AATCGTATAGGCATTTAACT+TGG | + | chr1.4:47940886-47940905 | MS.gene055663:intron | 30.0% | |
| ATGCGGAATAGAATGAATAA+GGG | - | chr1.4:47940664-47940683 | None:intergenic | 30.0% | |
| CACATTTAATGTTGTGATTC+AGG | + | chr1.4:47941104-47941123 | MS.gene055663:intron | 30.0% | |
| CTTAGGTTACATTATACCAT+TGG | + | chr1.4:47940705-47940724 | MS.gene055663:intron | 30.0% | |
| GTTACTTGAGTTAATCGTAT+AGG | + | chr1.4:47940874-47940893 | MS.gene055663:intron | 30.0% | |
| GTTGAAGATAAATATGTCAG+AGG | - | chr1.4:47940524-47940543 | None:intergenic | 30.0% | |
| TTAGCCAAAACTCACTATTA+CGG | + | chr1.4:47940397-47940416 | MS.gene055663:intron | 30.0% | |
| !! | GCATTAGGTTGAAAAATCTT+TGG | - | chr1.4:47941044-47941063 | None:intergenic | 30.0% |
| AGAAAAAGGAAGTGAGAAGA+AGG | - | chr1.4:47940687-47940706 | None:intergenic | 35.0% | |
| AGAATGAATAAGGGAAAGTG+AGG | - | chr1.4:47940655-47940674 | None:intergenic | 35.0% | |
| AGATAAATATGTCAGAGGAG+AGG | - | chr1.4:47940519-47940538 | None:intergenic | 35.0% | |
| AGGTTACATTATACCATTGG+TGG | + | chr1.4:47940708-47940727 | MS.gene055663:intron | 35.0% | |
| CAGTTATGAATACAAACCTG+AGG | - | chr1.4:47941257-47941276 | None:intergenic | 35.0% | |
| CATCACATTCATTCACACAA+TGG | + | chr1.4:47940548-47940567 | MS.gene055663:intron | 35.0% | |
| GATGCGGAATAGAATGAATA+AGG | - | chr1.4:47940665-47940684 | None:intergenic | 35.0% | |
| GCATAAGCATACTTACTTTG+TGG | - | chr1.4:47940789-47940808 | None:intergenic | 35.0% | |
| GGTTTACTAGAAACAATGTC+AGG | + | chr1.4:47940907-47940926 | MS.gene055663:intron | 35.0% | |
| GTGTGAATGAATGTGATGTT+TGG | - | chr1.4:47940546-47940565 | None:intergenic | 35.0% | |
| TAAACTCGTGTTTGAGAAAG+CGG | + | chr1.4:47940448-47940467 | MS.gene055663:intron | 35.0% | |
| TATCTCTTGTGAACAAGAAG+TGG | - | chr1.4:47940290-47940309 | None:intergenic | 35.0% | |
| ! | CTTCTCACTTCCTTTTTCTT+AGG | + | chr1.4:47940688-47940707 | MS.gene055663:intron | 35.0% |
| ! | TCAACCTAATGCAAAAGCAT+AGG | + | chr1.4:47941052-47941071 | MS.gene055663:intron | 35.0% |
| AACAAAGAAGCAAGCATGTG+GGG | - | chr1.4:47940813-47940832 | None:intergenic | 40.0% | |
| AGAACAAAGAAGCAAGCATG+TGG | - | chr1.4:47940815-47940834 | None:intergenic | 40.0% | |
| AGGAATAACCAACTGTTCGT+TGG | - | chr1.4:47940632-47940651 | None:intergenic | 40.0% | |
| ATATACCATCGACAGTCTCA+AGG | - | chr1.4:47941189-47941208 | None:intergenic | 40.0% | |
| ATGAATAAGGGAAAGTGAGG+AGG | - | chr1.4:47940652-47940671 | None:intergenic | 40.0% | |
| ATGATTTAACGAGCCACCAA+TGG | - | chr1.4:47940724-47940743 | None:intergenic | 40.0% | |
| ATTAGATAAACAACGCACGC+TGG | + | chr1.4:47941217-47941236 | MS.gene055663:intron | 40.0% | |
| GAACAAAGAAGCAAGCATGT+GGG | - | chr1.4:47940814-47940833 | None:intergenic | 40.0% | |
| TATACCATCGACAGTCTCAA+GGG | - | chr1.4:47941188-47941207 | None:intergenic | 40.0% | |
| TCTTGTGAACAAGAAGTGGA+GGG | - | chr1.4:47940286-47940305 | None:intergenic | 40.0% | |
| TGGAAGCAAGAAGAAGTAGA+AGG | - | chr1.4:47940612-47940631 | None:intergenic | 40.0% | |
| TGGCTCGTTAAATCATCTCA+AGG | + | chr1.4:47940728-47940747 | MS.gene055663:CDS | 40.0% | |
| TTACTTTGTGGAAGAGATGC+CGG | - | chr1.4:47940777-47940796 | None:intergenic | 40.0% | |
| TTCCTCACAATTGAAAAGCC+AGG | + | chr1.4:47940346-47940365 | MS.gene055663:CDS | 40.0% | |
| ! | AAGAAGAAGTAGAAGGTGTG+GGG | - | chr1.4:47940605-47940624 | None:intergenic | 40.0% |
| ! | CAAGAAGAAGTAGAAGGTGT+GGG | - | chr1.4:47940606-47940625 | None:intergenic | 40.0% |
| ! | GGATAATGCTTTTACTCCTC+AGG | + | chr1.4:47941238-47941257 | MS.gene055663:intron | 40.0% |
| ! | GTGACCTATGCTTTTGCATT+AGG | - | chr1.4:47941059-47941078 | None:intergenic | 40.0% |
| ! | TTCGCCGTAATAGTGAGTTT+TGG | - | chr1.4:47940404-47940423 | None:intergenic | 40.0% |
| !! | ACAATGGCTGATTTTACTCC+CGG | + | chr1.4:47940564-47940583 | MS.gene055663:intron | 40.0% |
| !! | TTCCTGGCTTTTCAATTGTG+AGG | - | chr1.4:47940351-47940370 | None:intergenic | 40.0% |
| AATCACCGTTTAGCCATCTC+CGG | + | chr1.4:47940755-47940774 | MS.gene055663:CDS | 45.0% | |
| AGGAAGTGAGAAGAAGGATG+CGG | - | chr1.4:47940681-47940700 | None:intergenic | 45.0% | |
| CTCTTGTGAACAAGAAGTGG+AGG | - | chr1.4:47940287-47940306 | None:intergenic | 45.0% | |
| CTGACAGAAGCTAACATTCC+TGG | - | chr1.4:47940367-47940386 | None:intergenic | 45.0% | |
| CTTGTGAACAAGAAGTGGAG+GGG | - | chr1.4:47940285-47940304 | None:intergenic | 45.0% | |
| GCAAGAAGAAGTAGAAGGTG+TGG | - | chr1.4:47940607-47940626 | None:intergenic | 45.0% | |
| GTTGTGATTCAGGGAAGAAG+CGG | + | chr1.4:47941114-47941133 | MS.gene055663:intron | 45.0% | |
| TATTACGGCGAAAGCAGAGT+TGG | + | chr1.4:47940412-47940431 | MS.gene055663:intron | 45.0% | |
| TCTTGCTTCCAACGAACAGT+TGG | + | chr1.4:47940621-47940640 | MS.gene055663:intron | 45.0% | |
| ! | AGAAAGCGGAAAAGCACGTT+GGG | + | chr1.4:47940462-47940481 | MS.gene055663:intron | 45.0% |
| ! | AGAAGAAGTAGAAGGTGTGG+GGG | - | chr1.4:47940604-47940623 | None:intergenic | 45.0% |
| AGACTCTCTCTCACGCTGTA+CGG | - | chr1.4:47940496-47940515 | None:intergenic | 50.0% | |
| AGATGCCGGAGATGGCTAAA+CGG | - | chr1.4:47940763-47940782 | None:intergenic | 50.0% | |
| ATTCAGGGAAGAAGCGGTTC+GGG | + | chr1.4:47941120-47941139 | MS.gene055663:intron | 50.0% | |
| GATTCAGGGAAGAAGCGGTT+CGG | + | chr1.4:47941119-47941138 | MS.gene055663:intron | 50.0% | |
| GGGGTGCAATTAGAAGAACC+GGG | - | chr1.4:47940585-47940604 | None:intergenic | 50.0% | |
| GTTTCCCTTGAGACTGTCGA+TGG | + | chr1.4:47941181-47941200 | MS.gene055663:CDS | 50.0% | |
| ! | GAGAAAGCGGAAAAGCACGT+TGG | + | chr1.4:47940461-47940480 | MS.gene055663:intron | 50.0% |
| ! | GGGGGTGCAATTAGAAGAAC+CGG | - | chr1.4:47940586-47940605 | None:intergenic | 50.0% |
| ! | GTTAGCTTCTGTCAGACGCA+AGG | + | chr1.4:47940372-47940391 | MS.gene055663:CDS | 50.0% |
| TGTGGAAGAGATGCCGGAGA+TGG | - | chr1.4:47940771-47940790 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 47940271 | 47941299 | 47940271 | ID=MS.gene055663 |
| chr1.4 | mRNA | 47940271 | 47941299 | 47940271 | ID=MS.gene055663.t1;Parent=MS.gene055663 |
| chr1.4 | exon | 47940271 | 47940393 | 47940271 | ID=MS.gene055663.t1.exon1;Parent=MS.gene055663.t1 |
| chr1.4 | CDS | 47940271 | 47940393 | 47940271 | ID=cds.MS.gene055663.t1;Parent=MS.gene055663.t1 |
| chr1.4 | exon | 47940710 | 47940792 | 47940710 | ID=MS.gene055663.t1.exon2;Parent=MS.gene055663.t1 |
| chr1.4 | CDS | 47940710 | 47940792 | 47940710 | ID=cds.MS.gene055663.t1;Parent=MS.gene055663.t1 |
| chr1.4 | exon | 47941126 | 47941202 | 47941126 | ID=MS.gene055663.t1.exon3;Parent=MS.gene055663.t1 |
| chr1.4 | CDS | 47941126 | 47941202 | 47941126 | ID=cds.MS.gene055663.t1;Parent=MS.gene055663.t1 |
| chr1.4 | exon | 47941292 | 47941299 | 47941292 | ID=MS.gene055663.t1.exon4;Parent=MS.gene055663.t1 |
| chr1.4 | CDS | 47941292 | 47941299 | 47941292 | ID=cds.MS.gene055663.t1;Parent=MS.gene055663.t1 |
| Gene Sequence |
| Protein sequence |
