AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055718

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055718.t1 MTR_0402s0040 71.569 204 57 1 12 215 2 204 4.59e-92 295
MS.gene055718.t1 MTR_0402s0040 56.187 299 73 5 397 693 248 490 2.19e-84 275
MS.gene055718.t1 MTR_7g113010 66.460 161 38 2 1 161 424 568 8.93e-56 201
MS.gene055718.t1 MTR_6g017200 72.093 129 35 1 4 131 25 153 7.88e-55 185
MS.gene055718.t1 MTR_1g057920 75.200 125 29 1 1 125 1 123 4.30e-54 182
MS.gene055718.t1 MTR_5g015480 64.286 154 51 1 12 165 36 185 5.40e-53 184
MS.gene055718.t1 MTR_2g070590 69.167 120 37 0 20 139 1 120 2.58e-49 169
MS.gene055718.t1 MTR_6g051425 31.494 308 180 8 35 317 13 314 3.95e-45 165
MS.gene055718.t1 MTR_0558s0020 33.074 257 153 4 40 277 40 296 1.31e-41 154
MS.gene055718.t1 MTR_8g446020 37.356 174 108 1 105 277 83 256 4.73e-34 131
MS.gene055718.t1 MTR_6g090505 34.597 211 123 3 81 276 20 230 2.58e-33 132
MS.gene055718.t1 MTR_0137s0040 56.731 104 45 0 1 104 1 104 1.57e-30 116
MS.gene055718.t1 MTR_1g070355 32.203 236 148 5 42 271 80 309 1.73e-30 127
MS.gene055718.t1 MTR_1g083640 29.386 228 142 4 40 248 55 282 6.96e-28 114
MS.gene055718.t1 MTR_4g035820 30.496 282 180 9 9 281 50 324 8.84e-26 113
MS.gene055718.t1 MTR_1g050985 76.667 60 14 0 106 165 39 98 3.05e-25 100
MS.gene055718.t1 MTR_7g074790 39.429 175 84 4 33 206 7 160 5.62e-24 101
MS.gene055718.t1 MTR_4g102720 33.140 172 79 3 33 204 4 139 6.45e-23 98.2
MS.gene055718.t1 MTR_0291s0060 63.514 74 27 0 13 86 12 85 3.45e-22 91.7
MS.gene055718.t1 MTR_7g446520 30.165 242 151 4 42 282 94 318 2.32e-21 96.3
MS.gene055718.t1 MTR_5g039760 35.294 204 102 7 32 232 6 182 2.97e-20 89.7
MS.gene055718.t1 MTR_7g446480 35.227 176 111 2 112 285 140 314 3.91e-20 92.4
MS.gene055718.t1 MTR_7g061980 41.176 119 66 3 30 147 4 119 1.75e-19 85.1
MS.gene055718.t1 MTR_7g446510 34.659 176 112 2 112 285 155 329 2.77e-19 90.1
MS.gene055718.t1 MTR_0337s0040 28.902 173 110 3 82 242 51 222 4.21e-19 87.4
MS.gene055718.t1 MTR_5g076070 25.991 227 162 4 42 265 59 282 7.11e-18 85.1
MS.gene055718.t1 MTR_1g019850 33.742 163 101 2 7 169 41 196 5.73e-17 81.3
MS.gene055718.t1 MTR_1g091013 55.128 78 24 1 27 104 3 69 1.14e-16 75.5
MS.gene055718.t1 MTR_1g008290 33.566 143 90 3 89 230 3 141 2.33e-16 77.0
MS.gene055718.t1 MTR_1g086270 56.061 66 21 2 331 396 1 58 3.47e-16 75.5
MS.gene055718.t1 MTR_2g014100 24.623 199 130 3 40 219 48 245 1.04e-15 77.8
MS.gene055718.t1 MTR_4g067290 41.667 108 57 3 35 140 55 158 6.63e-15 75.1
MS.gene055718.t1 MTR_4g067290 41.667 108 57 3 35 140 55 158 6.80e-15 75.1
MS.gene055718.t1 MTR_4g067290 41.667 108 57 3 35 140 75 178 1.08e-14 75.1
MS.gene055718.t1 MTR_4g067290 41.667 108 57 3 35 140 75 178 1.09e-14 75.1
MS.gene055718.t1 MTR_4g067290 41.667 108 57 3 35 140 73 176 1.10e-14 75.1
MS.gene055718.t1 MTR_4g067290 41.667 108 57 3 35 140 115 218 1.37e-14 75.5
MS.gene055718.t1 MTR_2g098920 38.889 108 60 3 35 140 79 182 4.86e-14 73.2
MS.gene055718.t1 MTR_5g045210 40.449 89 53 0 488 576 20 108 6.62e-14 71.6
MS.gene055718.t1 MTR_3g006370 38.095 105 63 2 35 138 49 152 2.13e-12 67.8
MS.gene055718.t1 MTR_3g450850 23.077 247 178 5 41 276 52 297 3.84e-12 68.9
MS.gene055718.t1 MTR_2g099430 38.202 89 53 1 59 147 26 112 4.83e-12 63.9
MS.gene055718.t1 MTR_6g022080 26.582 158 113 2 42 197 91 247 7.20e-12 66.6
MS.gene055718.t1 MTR_1g055240 30.769 117 81 0 104 220 33 149 8.00e-12 64.3
MS.gene055718.t1 MTR_2g098880 33.858 127 77 4 22 142 44 169 2.31e-11 65.1
MS.gene055718.t1 MTR_2g098880 33.858 127 77 4 22 142 56 181 2.64e-11 65.1
MS.gene055718.t1 MTR_3g008240 25.153 163 104 3 40 184 40 202 4.23e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055718.t1 AT4G38180 37.752 347 210 4 23 365 60 404 4.35e-74 255
MS.gene055718.t1 AT4G38180 30.612 196 130 4 380 570 499 693 9.81e-15 78.6
MS.gene055718.t1 AT4G15090 33.433 335 219 4 33 365 48 380 9.25e-59 213
MS.gene055718.t1 AT4G15090 29.630 189 123 3 389 573 484 666 7.38e-14 75.9
MS.gene055718.t1 AT4G15090 33.433 335 219 4 33 365 48 380 9.25e-59 213
MS.gene055718.t1 AT4G15090 29.630 189 123 3 389 573 484 666 7.38e-14 75.9
MS.gene055718.t1 AT4G15090 33.433 335 219 4 33 365 48 380 9.25e-59 213
MS.gene055718.t1 AT4G15090 29.630 189 123 3 389 573 484 666 7.38e-14 75.9
MS.gene055718.t1 AT4G15090 33.433 335 219 4 33 365 48 380 9.25e-59 213
MS.gene055718.t1 AT4G15090 29.630 189 123 3 389 573 484 666 7.38e-14 75.9
MS.gene055718.t1 AT4G15090 33.433 335 219 4 33 365 48 380 9.25e-59 213
MS.gene055718.t1 AT4G15090 29.630 189 123 3 389 573 484 666 7.38e-14 75.9
MS.gene055718.t1 AT1G76320 33.642 324 202 6 40 361 1 313 9.96e-53 194
MS.gene055718.t1 AT1G76320 30.857 175 103 6 389 556 421 584 8.91e-14 75.5
MS.gene055718.t1 AT1G76320 33.642 324 202 6 40 361 1 313 2.26e-52 194
MS.gene055718.t1 AT1G76320 30.857 175 103 6 389 556 421 584 5.03e-14 76.3
MS.gene055718.t1 AT1G76320 33.642 324 202 6 40 361 1 313 2.31e-52 194
MS.gene055718.t1 AT1G76320 30.857 175 103 6 389 556 421 584 5.40e-14 76.3
MS.gene055718.t1 AT1G76320 33.642 324 202 6 40 361 1 313 2.31e-52 194
MS.gene055718.t1 AT1G76320 30.857 175 103 6 389 556 421 584 5.40e-14 76.3
MS.gene055718.t1 AT5G28530 25.085 590 341 15 36 531 55 637 1.76e-49 185
MS.gene055718.t1 AT5G28530 25.085 590 341 15 36 531 55 637 1.76e-49 185
MS.gene055718.t1 AT1G10240 32.831 332 210 9 29 351 41 368 1.93e-49 184
MS.gene055718.t1 AT1G10240 32.831 332 210 9 29 351 41 368 1.93e-49 184
MS.gene055718.t1 AT3G22170 31.832 333 189 7 33 351 67 375 5.38e-47 179
MS.gene055718.t1 AT3G22170 34.270 178 94 6 389 556 493 657 3.18e-18 90.1
MS.gene055718.t1 AT3G22170 31.832 333 189 7 33 351 67 375 5.38e-47 179
MS.gene055718.t1 AT3G22170 34.270 178 94 6 389 556 493 657 3.18e-18 90.1
MS.gene055718.t1 AT2G27110 31.818 352 204 10 28 373 41 362 1.17e-40 160
MS.gene055718.t1 AT2G27110 34.694 196 118 6 380 569 444 635 3.10e-20 96.3
MS.gene055718.t1 AT2G27110 31.818 352 204 10 28 373 41 362 1.17e-40 160
MS.gene055718.t1 AT2G27110 34.694 196 118 6 380 569 444 635 3.10e-20 96.3
MS.gene055718.t1 AT3G06250 29.063 320 184 5 35 352 189 467 2.22e-40 159
MS.gene055718.t1 AT3G06250 30.435 161 104 4 413 568 601 758 3.73e-13 73.6
MS.gene055718.t1 AT3G06250 29.063 320 184 5 35 352 189 467 2.22e-40 159
MS.gene055718.t1 AT3G06250 30.435 161 104 4 413 568 601 758 3.73e-13 73.6
MS.gene055718.t1 AT3G06250 29.063 320 184 5 35 352 189 467 2.22e-40 159
MS.gene055718.t1 AT3G06250 30.435 161 104 4 413 568 601 758 3.73e-13 73.6
MS.gene055718.t1 AT2G32250 29.560 318 175 9 37 351 39 310 5.43e-38 151
MS.gene055718.t1 AT2G32250 25.941 239 160 7 339 568 375 605 1.22e-12 72.0
MS.gene055718.t1 AT2G32250 28.882 322 172 9 37 351 39 310 6.61e-38 151
MS.gene055718.t1 AT2G32250 25.941 239 160 7 339 568 375 605 9.35e-13 72.4
MS.gene055718.t1 AT2G32250 28.882 322 172 9 37 351 39 310 6.61e-38 151
MS.gene055718.t1 AT2G32250 25.941 239 160 7 339 568 375 605 9.35e-13 72.4
MS.gene055718.t1 AT2G32250 28.882 322 172 9 37 351 39 310 6.61e-38 151
MS.gene055718.t1 AT2G32250 25.941 239 160 7 339 568 375 605 9.35e-13 72.4
MS.gene055718.t1 AT2G32250 28.882 322 172 9 37 351 39 310 7.45e-38 151
MS.gene055718.t1 AT2G32250 25.941 239 160 7 339 568 375 605 1.04e-12 72.0
MS.gene055718.t1 AT2G32250 28.882 322 172 9 37 351 39 310 7.45e-38 151
MS.gene055718.t1 AT2G32250 25.941 239 160 7 339 568 375 605 1.04e-12 72.0
MS.gene055718.t1 AT2G32250 28.840 319 170 9 40 351 1 269 7.73e-38 151
MS.gene055718.t1 AT2G32250 25.941 239 160 7 339 568 334 564 1.12e-12 72.0
MS.gene055718.t1 AT2G32250 28.840 319 170 9 40 351 1 269 9.01e-38 151
MS.gene055718.t1 AT2G32250 27.083 240 156 9 339 568 334 564 1.18e-12 72.0
MS.gene055718.t1 AT5G28530 27.419 372 234 10 36 383 55 414 1.24e-37 150
MS.gene055718.t1 AT5G28530 29.252 147 99 2 390 531 495 641 1.70e-11 68.2
MS.gene055718.t1 AT5G18960 29.664 327 190 8 29 353 204 492 1.62e-37 150
MS.gene055718.t1 AT5G18960 30.435 161 104 4 413 568 625 782 1.00e-13 75.5
MS.gene055718.t1 AT5G18960 29.664 327 190 8 29 353 204 492 1.62e-37 150
MS.gene055718.t1 AT5G18960 30.435 161 104 4 413 568 625 782 1.00e-13 75.5
MS.gene055718.t1 AT1G80010 27.093 454 283 10 21 435 80 524 7.97e-36 145
MS.gene055718.t1 AT1G52520 29.969 327 194 9 36 351 85 387 3.26e-35 142
MS.gene055718.t1 AT2G27110 39.877 163 94 2 215 373 55 217 3.28e-32 133
MS.gene055718.t1 AT2G27110 34.694 196 118 6 380 569 299 490 2.86e-20 96.3
MS.gene055718.t1 AT4G19990 27.824 363 208 8 42 387 27 352 1.57e-31 132
MS.gene055718.t1 AT4G19990 31.548 168 89 4 413 566 455 610 4.56e-11 66.6
MS.gene055718.t1 AT4G19990 27.824 363 208 8 42 387 41 366 1.83e-31 131
MS.gene055718.t1 AT4G19990 31.548 168 89 4 413 566 469 624 4.81e-11 66.6
MS.gene055718.t1 AT4G19990 27.824 363 208 8 42 387 27 352 1.94e-31 131
MS.gene055718.t1 AT4G19990 31.034 174 94 4 413 572 455 616 5.01e-11 66.6
MS.gene055718.t1 AT4G19990 27.824 363 208 8 42 387 27 352 1.94e-31 131
MS.gene055718.t1 AT4G19990 31.034 174 94 4 413 572 455 616 5.01e-11 66.6
MS.gene055718.t1 AT4G19990 27.824 363 208 8 42 387 27 352 2.06e-31 131
MS.gene055718.t1 AT4G19990 31.548 168 89 4 413 566 455 610 4.52e-11 66.6
MS.gene055718.t1 AT4G19990 27.824 363 208 8 42 387 27 352 2.06e-31 131
MS.gene055718.t1 AT4G19990 31.548 168 89 4 413 566 455 610 4.52e-11 66.6
MS.gene055718.t1 AT4G19990 29.167 288 163 7 104 387 45 295 4.10e-25 111
MS.gene055718.t1 AT4G19990 31.548 168 89 4 413 566 398 553 4.75e-11 66.6
MS.gene055718.t1 AT4G38170 31.795 195 119 5 204 396 10 192 6.99e-25 110
MS.gene055718.t1 AT4G38170 32.168 143 91 3 380 517 264 405 1.07e-16 84.3
MS.gene055718.t1 AT5G28530 21.739 575 316 20 36 531 55 574 1.67e-21 99.8
MS.gene055718.t1 AT2G43280 39.316 117 67 3 34 147 20 135 9.14e-16 77.0
MS.gene055718.t1 AT3G59470 35.915 142 79 4 9 140 33 172 4.59e-14 73.2
MS.gene055718.t1 AT3G59470 35.915 142 79 4 9 140 33 172 4.59e-14 73.2
MS.gene055718.t1 AT3G59470 36.620 142 78 4 9 140 45 184 5.41e-14 73.2
MS.gene055718.t1 AT4G12850 35.714 126 74 3 36 161 11 129 2.52e-12 65.5
MS.gene055718.t1 AT5G28530 29.252 147 99 2 390 531 432 578 1.69e-11 68.2
MS.gene055718.t1 AT2G43280 37.000 100 59 3 51 147 2 100 2.01e-11 63.5
MS.gene055718.t1 AT4G12850 38.095 105 61 2 35 139 10 110 2.21e-11 62.8
MS.gene055718.t1 AT4G12850 37.143 105 62 2 35 139 10 110 6.44e-11 62.4

Find 132 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATAACAAATATATTT+TGG 0.083882 7.4:+20023307 MS.gene055718:CDS
AGACAATTCTGTTGATAAAT+TGG 0.199895 7.4:+20022618 MS.gene055718:CDS
CATGAATCCGTCGTCTATTT+TGG 0.206115 7.4:+20022594 None:intergenic
GCACAAATGATCAATGATTA+TGG 0.212099 7.4:+20023336 MS.gene055718:CDS
CATTTCATGGATTGTGTACT+TGG 0.244889 7.4:+20023619 MS.gene055718:CDS
TTTGGTATTCGTCGAGAATA+TGG 0.265133 7.4:+20022775 MS.gene055718:CDS
ATGTCCTCCAATGATTAAAT+TGG 0.279912 7.4:+20024448 MS.gene055718:CDS
ATATATTCATGCAAATCTTT+TGG 0.296908 7.4:-20023105 None:intergenic
CACTGTACCGCTTCATGAAA+TGG 0.307019 7.4:-20024421 None:intergenic
ACAAACATCACAAATTATAA+TGG 0.314128 7.4:+20023062 MS.gene055718:CDS
AGAATTTGCTTCGAACAAAA+AGG 0.321129 7.4:+20023181 MS.gene055718:CDS
CGCTTTAGTATATAGTGTTC+AGG 0.332129 7.4:-20024326 None:intergenic
AATGAGTATAGTAAAAGAAT+TGG 0.336174 7.4:+20022751 MS.gene055718:CDS
CACCACATACAAGACAAATA+AGG 0.340827 7.4:+20023386 MS.gene055718:CDS
ATTTCACTTGATCGAAAGAT+AGG 0.345588 7.4:+20022940 MS.gene055718:CDS
GTTATGCCGGAGACATTTCA+TGG 0.359089 7.4:+20023606 MS.gene055718:CDS
CTGTTGGCCAATTTAATCAT+TGG 0.359632 7.4:-20024455 None:intergenic
ATTATAATGGGAGATGAATC+TGG 0.362761 7.4:+20023075 MS.gene055718:CDS
CGGCCGTTGGGAGTATTTGT+TGG 0.367992 7.4:+20023414 MS.gene055718:CDS
CTTCGCTTGATTGTGTCCCT+TGG 0.383017 7.4:-20024998 None:intergenic
ATATAAGACAAAACGCTCTA+AGG 0.384570 7.4:+20023643 MS.gene055718:CDS
TTTGTCTTCATTGTGCCCCT+TGG 0.391077 7.4:-20025052 None:intergenic
TATAGTAAAAGAATTGGATT+TGG 0.393932 7.4:+20022757 MS.gene055718:CDS
CTTGACAACGGTTTCCATGC+TGG 0.393949 7.4:-20024636 None:intergenic
GATATTCCTCGTATTCCTCT+TGG 0.399365 7.4:-20024112 None:intergenic
GCTGGACTCTTCATAAACTT+TGG 0.408472 7.4:-20024513 None:intergenic
AGAATCAAGTGGAATGGTTC+AGG 0.410213 7.4:+20024577 MS.gene055718:CDS
CAAACATCACAAATTATAAT+GGG 0.416760 7.4:+20023063 MS.gene055718:CDS
CTCAATTCAATTACTCTCAA+TGG 0.424494 7.4:+20024900 MS.gene055718:CDS
TGTAAAAGAGAGGACAGTTA+TGG 0.424574 7.4:+20024193 MS.gene055718:CDS
AGGATGGACTCACTAAAGTA+TGG 0.430267 7.4:+20023201 MS.gene055718:CDS
CAATCAAGCGAAGTTGATAA+TGG 0.430767 7.4:+20025008 MS.gene055718:CDS
ATCATGTGAACATACTCTTC+CGG 0.437349 7.4:-20023012 None:intergenic
CATCATCAAGCTTCAATTCA+AGG 0.437452 7.4:+20024927 MS.gene055718:CDS
ACAAGACAAATAAGGGTTAT+CGG 0.444271 7.4:+20023394 MS.gene055718:CDS
TGCATAAAGGTGTTGAAAGA+AGG 0.445593 7.4:+20024143 MS.gene055718:CDS
ACCACATACAAGACAAATAA+GGG 0.447196 7.4:+20023387 MS.gene055718:CDS
CTCTATCCCACCAACCTGCT+TGG 0.449496 7.4:-20023130 None:intergenic
GACAAAAGACGTCAACCGAC+TGG 0.454042 7.4:+20022871 MS.gene055718:CDS
GTAAAAGAGAGGACAGTTAT+GGG 0.455513 7.4:+20024194 MS.gene055718:CDS
GGTTGGGAAGTAATCAAGAT+TGG 0.457422 7.4:+20024366 MS.gene055718:CDS
TCAAATAACCACTGAAAAGA+AGG 0.457713 7.4:-20023498 None:intergenic
CACAAATGATCAATGATTAT+GGG 0.462910 7.4:+20023337 MS.gene055718:CDS
TGAAAGTCATAAAAGAAAGA+TGG 0.464163 7.4:-20023270 None:intergenic
ACTGTACCGCTTCATGAAAT+GGG 0.469454 7.4:-20024420 None:intergenic
GGGTATTTCTTTAGTTATGC+CGG 0.469549 7.4:+20023593 MS.gene055718:CDS
AGCATCTTGATCCGTTAGAA+TGG 0.469910 7.4:-20023559 None:intergenic
AACCGGGTGTAATGCACGTA+TGG 0.472531 7.4:+20022915 MS.gene055718:CDS
TGCCGACATCAAACCTCGCT+TGG 0.474766 7.4:+20022687 MS.gene055718:CDS
ATGAGATTCTGAAGCTCTGT+TGG 0.476036 7.4:-20024471 None:intergenic
AATGGGCATGGACTTATGTT+CGG 0.478812 7.4:+20023835 MS.gene055718:CDS
ATATCAAGACACTTGCATAA+AGG 0.478993 7.4:+20024130 MS.gene055718:CDS
ATCAATGATTATGGGTACTT+TGG 0.481641 7.4:+20023345 MS.gene055718:CDS
GAAACCGTTGTCAAGGCAAA+AGG 0.483784 7.4:+20024644 MS.gene055718:CDS
GGGTGTAAACATTTCCGGCT+TGG 0.486090 7.4:-20024070 None:intergenic
ACCCTTATTTGTCTTGTATG+TGG 0.486140 7.4:-20023388 None:intergenic
AATAAGGGTTATCGGCCGTT+GGG 0.489965 7.4:+20023402 MS.gene055718:CDS
CATTGAGAGTAATTGAATTG+AGG 0.493852 7.4:-20024899 None:intergenic
TTTATCATTTCCAAGTGATA+CGG 0.494676 7.4:-20024603 None:intergenic
TTGGTATTCGTCGAGAATAT+GGG 0.496079 7.4:+20022776 MS.gene055718:CDS
TGGACAAGAGAGGCAAGGTT+GGG 0.506606 7.4:+20024350 MS.gene055718:CDS
TCTACGGCAGAAACTAGAAC+CGG 0.508196 7.4:+20022898 MS.gene055718:CDS
CAAAATAGTTGAAGATATGT+TGG 0.512003 7.4:+20024535 MS.gene055718:CDS
CATACTCTTCCGGCTGAAGA+AGG 0.514601 7.4:-20023002 None:intergenic
TTGGCCAATTTAATCATTGG+AGG 0.515834 7.4:-20024452 None:intergenic
CAATGTCAAGCTACAATCCA+AGG 0.517257 7.4:+20025035 MS.gene055718:CDS
CTATGTCAAACTGCAATCCA+AGG 0.518552 7.4:+20024981 MS.gene055718:CDS
ACCTAGATTGAAAATGAAGA+AGG 0.518870 7.4:+20024034 MS.gene055718:CDS
ATGGACAAGAGAGGCAAGGT+TGG 0.520495 7.4:+20024349 MS.gene055718:CDS
GTTGGTGGGATAGAGATGGT+TGG 0.521078 7.4:+20023138 MS.gene055718:CDS
CACAATCCATGAAATGTCTC+CGG 0.521781 7.4:-20023612 None:intergenic
AAATAAGGGTTATCGGCCGT+TGG 0.523633 7.4:+20023401 MS.gene055718:CDS
CAAGATGCTGCGATGGCAAA+GGG 0.528327 7.4:+20023573 MS.gene055718:CDS
GAAGTAATCAAGATTGGAAA+GGG 0.535116 7.4:+20024372 MS.gene055718:CDS
CAATATTCTTGAACAAAATG+AGG 0.537039 7.4:+20022645 MS.gene055718:CDS
GTTCGGAAAACATTCACTGC+AGG 0.544889 7.4:+20023852 MS.gene055718:CDS
ACAAAAGACGTCAACCGACT+GGG 0.550284 7.4:+20022872 MS.gene055718:CDS
TCAAGATGCTGCGATGGCAA+AGG 0.551791 7.4:+20023572 MS.gene055718:CDS
CTCTCATCGTCGCATATCTG+AGG 0.552258 7.4:+20023038 MS.gene055718:CDS
TCACTAAAGTATGGTGAAGT+GGG 0.555591 7.4:+20023210 MS.gene055718:CDS
GCATGAATATATGTCCAAGC+AGG 0.557373 7.4:+20023116 MS.gene055718:CDS
TAGTCATGCTTTGAAAGTGT+TGG 0.559284 7.4:+20024280 MS.gene055718:CDS
GTAATTGAATTGAGGTCAAG+TGG 0.560596 7.4:-20024891 None:intergenic
TTTCACTTGATCGAAAGATA+GGG 0.567868 7.4:+20022941 MS.gene055718:CDS
AACACTATATACTAAAGCGA+TGG 0.568133 7.4:+20024330 MS.gene055718:CDS
TCAACCGACTGGGGAGTCTA+CGG 0.568520 7.4:+20022882 MS.gene055718:CDS
GCTCGAATGCTCGTCCAAGC+CGG 0.569311 7.4:+20024056 MS.gene055718:CDS
CAGCATGGAAACCGTTGTCA+AGG 0.570861 7.4:+20024637 MS.gene055718:CDS
GGAAGTAATCAAGATTGGAA+AGG 0.571376 7.4:+20024371 MS.gene055718:CDS
TATATGTCCAAGCAGGTTGG+TGG 0.571492 7.4:+20023123 MS.gene055718:CDS
GACAGTTATGGGAAATCCTA+CGG 0.576210 7.4:+20024205 MS.gene055718:CDS
CACTTTAAAAGAGCTGTCAA+TGG 0.576395 7.4:+20023963 MS.gene055718:intron
ACACAACCACCTTCTTCAGC+CGG 0.580649 7.4:+20022993 MS.gene055718:CDS
TAAAGAGAAAGAAGCCAGCA+TGG 0.582901 7.4:+20024622 MS.gene055718:CDS
ATCATCAAGCTTCAATTCAA+GGG 0.586404 7.4:+20024928 MS.gene055718:CDS
AACGGATCAAGATGCTGCGA+TGG 0.589430 7.4:+20023566 MS.gene055718:CDS
CTCACTAAAGTATGGTGAAG+TGG 0.589479 7.4:+20023209 MS.gene055718:CDS
GTTCAGGTAACCGTATCACT+TGG 0.589853 7.4:+20024593 MS.gene055718:CDS
ACAGGAAAACCAGTCGTTAG+CGG 0.592357 7.4:+20024778 MS.gene055718:CDS
AGATTCACATGCTACAAAGA+AGG 0.592653 7.4:+20022835 MS.gene055718:CDS
CTTGAACAAAATGAGGAACA+TGG 0.594103 7.4:+20022652 MS.gene055718:CDS
AAATCTTGGATTGAGAAAGA+AGG 0.598872 7.4:+20024710 MS.gene055718:CDS
AAAGCCTAAAACCATTCTAA+CGG 0.600846 7.4:+20023548 MS.gene055718:CDS
ACCGGGTGTAATGCACGTAT+GGG 0.604247 7.4:+20022916 MS.gene055718:CDS
GGATATGATATTTGAATCCG+AGG 0.604669 7.4:+20022708 MS.gene055718:CDS
TGAAATCCCATTTCATGAAG+CGG 0.605325 7.4:+20024414 MS.gene055718:CDS
ACCCATACGTGCATTACACC+CGG 0.613296 7.4:-20022917 None:intergenic
GAATATATGTCCAAGCAGGT+TGG 0.614674 7.4:+20023120 MS.gene055718:CDS
ATATGTCCAAGCAGGTTGGT+GGG 0.619945 7.4:+20023124 MS.gene055718:CDS
GCAGGTTGGTGGGATAGAGA+TGG 0.629905 7.4:+20023134 MS.gene055718:CDS
TCAAATATCATATCCAAGCG+AGG 0.630717 7.4:-20022700 None:intergenic
AATGTCAAGCTACAATCCAA+GGG 0.633905 7.4:+20025036 MS.gene055718:CDS
AAAGAGCTGTCAATGGTAAG+CGG 0.639455 7.4:+20023970 MS.gene055718:intron
ACTCTTCCGGCTGAAGAAGG+TGG 0.641594 7.4:-20022999 None:intergenic
TCGAAAGATAGGGAAATACA+AGG 0.644061 7.4:+20022951 MS.gene055718:CDS
CTACGGCAGAAACTAGAACC+GGG 0.648731 7.4:+20022899 MS.gene055718:CDS
AGCGATGGACAAGAGAGGCA+AGG 0.649888 7.4:+20024345 MS.gene055718:CDS
ATTATGATAATGTAAAAGAG+AGG 0.650123 7.4:+20024183 MS.gene055718:CDS
TGTCGTAAATTTGAGACACA+CGG 0.654078 7.4:+20024248 MS.gene055718:CDS
TATGTCAAACTGCAATCCAA+GGG 0.654427 7.4:+20024982 MS.gene055718:CDS
AGTCCAACAAATACTCCCAA+CGG 0.659244 7.4:-20023417 None:intergenic
AATATTTGAGGAATTCCAAG+AGG 0.661269 7.4:+20024097 MS.gene055718:CDS
GGATGGAGAATCAAGTGGAA+TGG 0.662094 7.4:+20024571 MS.gene055718:CDS
ATCCAAGCGAGGTTTGATGT+CGG 0.664750 7.4:-20022689 None:intergenic
TCTGCCGTAGACTCCCCAGT+CGG 0.665919 7.4:-20022886 None:intergenic
CAAAAGACGTCAACCGACTG+GGG 0.676456 7.4:+20022873 MS.gene055718:CDS
TGTGTGGATGGAGAATCAAG+TGG 0.678307 7.4:+20024566 MS.gene055718:CDS
ACTAAAGCGATGGACAAGAG+AGG 0.678959 7.4:+20024340 MS.gene055718:CDS
GGAATTCCAAGAGGAATACG+AGG 0.680926 7.4:+20024106 MS.gene055718:CDS
AAATTCATAAGCATCTACCT+CGG 0.685544 7.4:-20022725 None:intergenic
TTGTCTACCAAAATAGACGA+CGG 0.691097 7.4:-20022601 None:intergenic
ATGTCAAGCTACAATCCAAG+GGG 0.727067 7.4:+20025037 MS.gene055718:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCAAATAACAAATATATTT+TGG + chr7.4:20023307-20023326 MS.gene055718:CDS 15.0%
!!! GCAAATAACAAATATATTTT+GGG + chr7.4:20023308-20023327 MS.gene055718:CDS 15.0%
!! AATGAGTATAGTAAAAGAAT+TGG + chr7.4:20022751-20022770 MS.gene055718:CDS 20.0%
!! ACAAACATCACAAATTATAA+TGG + chr7.4:20023062-20023081 MS.gene055718:CDS 20.0%
!! CAAACATCACAAATTATAAT+GGG + chr7.4:20023063-20023082 MS.gene055718:CDS 20.0%
!! TATAGTAAAAGAATTGGATT+TGG + chr7.4:20022757-20022776 MS.gene055718:CDS 20.0%
!! TATTTAAATCTGACTTCAAA+TGG - chr7.4:20023926-20023945 None:intergenic 20.0%
!!! ATATATTCATGCAAATCTTT+TGG - chr7.4:20023108-20023127 None:intergenic 20.0%
!!! ATTATGATAATGTAAAAGAG+AGG + chr7.4:20024183-20024202 MS.gene055718:CDS 20.0%
!!! TTTCTTTTATGACTTTCAAA+TGG + chr7.4:20023275-20023294 MS.gene055718:CDS 20.0%
!!! TTTTCGATTTAAGGAAAAAT+GGG + chr7.4:20023818-20023837 MS.gene055718:intron 20.0%
!!! TTTTTCGATTTAAGGAAAAA+TGG + chr7.4:20023817-20023836 MS.gene055718:intron 20.0%
! CAAAATAGTTGAAGATATGT+TGG + chr7.4:20024535-20024554 MS.gene055718:CDS 25.0%
! CAATATTCTTGAACAAAATG+AGG + chr7.4:20022645-20022664 MS.gene055718:CDS 25.0%
! CACAAATGATCAATGATTAT+GGG + chr7.4:20023337-20023356 MS.gene055718:CDS 25.0%
! GAATAAAAGCAAAAAAGACA+GGG + chr7.4:20022798-20022817 MS.gene055718:CDS 25.0%
! TGAAAGTCATAAAAGAAAGA+TGG - chr7.4:20023273-20023292 None:intergenic 25.0%
! TTTATCATTTCCAAGTGATA+CGG - chr7.4:20024606-20024625 None:intergenic 25.0%
!! AAGATGAATTTTTGAATGCA+TGG + chr7.4:20023742-20023761 MS.gene055718:intron 25.0%
!! AGACAATTCTGTTGATAAAT+TGG + chr7.4:20022618-20022637 MS.gene055718:CDS 25.0%
!! CTGTGATTTTCTCTTTTTTT+GGG - chr7.4:20024762-20024781 None:intergenic 25.0%
!! GGAATTCCTCAAATATTTTT+GGG - chr7.4:20024094-20024113 None:intergenic 25.0%
!! TGGAATTCCTCAAATATTTT+TGG - chr7.4:20024095-20024114 None:intergenic 25.0%
!!! GAATTCCTCAAATATTTTTG+GGG - chr7.4:20024093-20024112 None:intergenic 25.0%
!!! GCATATGATTTTTCGATTTA+AGG + chr7.4:20023809-20023828 MS.gene055718:intron 25.0%
AAAGCCTAAAACCATTCTAA+CGG + chr7.4:20023548-20023567 MS.gene055718:CDS 30.0%
AAATCTTGGATTGAGAAAGA+AGG + chr7.4:20024710-20024729 MS.gene055718:CDS 30.0%
AAATTCATAAGCATCTACCT+CGG - chr7.4:20022728-20022747 None:intergenic 30.0%
AACACTATATACTAAAGCGA+TGG + chr7.4:20024330-20024349 MS.gene055718:CDS 30.0%
AATATTTGAGGAATTCCAAG+AGG + chr7.4:20024097-20024116 MS.gene055718:CDS 30.0%
ACAAGACAAATAAGGGTTAT+CGG + chr7.4:20023394-20023413 MS.gene055718:CDS 30.0%
ACCACATACAAGACAAATAA+GGG + chr7.4:20023387-20023406 MS.gene055718:CDS 30.0%
ACCTAGATTGAAAATGAAGA+AGG + chr7.4:20024034-20024053 MS.gene055718:CDS 30.0%
ATATAAGACAAAACGCTCTA+AGG + chr7.4:20023643-20023662 MS.gene055718:CDS 30.0%
ATATCAAGACACTTGCATAA+AGG + chr7.4:20024130-20024149 MS.gene055718:CDS 30.0%
ATCAATGATTATGGGTACTT+TGG + chr7.4:20023345-20023364 MS.gene055718:CDS 30.0%
ATCATCAAGCTTCAATTCAA+GGG + chr7.4:20024928-20024947 MS.gene055718:CDS 30.0%
ATGTCCTCCAATGATTAAAT+TGG + chr7.4:20024448-20024467 MS.gene055718:CDS 30.0%
ATTTCACTTGATCGAAAGAT+AGG + chr7.4:20022940-20022959 MS.gene055718:CDS 30.0%
CATTGAGAGTAATTGAATTG+AGG - chr7.4:20024902-20024921 None:intergenic 30.0%
CCAAAAAAAGAGAAAATCAC+AGG + chr7.4:20024760-20024779 MS.gene055718:CDS 30.0%
CTAAAAAACGAGCTAAATCT+TGG + chr7.4:20024696-20024715 MS.gene055718:CDS 30.0%
CTCAATTCAATTACTCTCAA+TGG + chr7.4:20024900-20024919 MS.gene055718:CDS 30.0%
GAAGTAATCAAGATTGGAAA+GGG + chr7.4:20024372-20024391 MS.gene055718:CDS 30.0%
GCACAAATGATCAATGATTA+TGG + chr7.4:20023336-20023355 MS.gene055718:CDS 30.0%
GGAATAAAAGCAAAAAAGAC+AGG + chr7.4:20022797-20022816 MS.gene055718:CDS 30.0%
TCAAATAACCACTGAAAAGA+AGG - chr7.4:20023501-20023520 None:intergenic 30.0%
TTACACCCCAAAAATATTTG+AGG + chr7.4:20024085-20024104 MS.gene055718:CDS 30.0%
TTTCACTTGATCGAAAGATA+GGG + chr7.4:20022941-20022960 MS.gene055718:CDS 30.0%
! AAACAATACCTTCTTTTCAG+TGG + chr7.4:20023490-20023509 MS.gene055718:CDS 30.0%
! AGAATTTGCTTCGAACAAAA+AGG + chr7.4:20023181-20023200 MS.gene055718:CDS 30.0%
! ATTATAATGGGAGATGAATC+TGG + chr7.4:20023075-20023094 MS.gene055718:CDS 30.0%
! CATATCTTCAACTATTTTGC+TGG - chr7.4:20024534-20024553 None:intergenic 30.0%
! CATCGACAAACAATCATTTT+TGG + chr7.4:20023447-20023466 MS.gene055718:CDS 30.0%
! CCTGTGATTTTCTCTTTTTT+TGG - chr7.4:20024763-20024782 None:intergenic 30.0%
! GAAACTTTTCTCAAAGCAAT+GGG + chr7.4:20023519-20023538 MS.gene055718:CDS 30.0%
! GCTTACTCTTTTGATATAGA+TGG - chr7.4:20023677-20023696 None:intergenic 30.0%
! TGAAACTTTTCTCAAAGCAA+TGG + chr7.4:20023518-20023537 MS.gene055718:CDS 30.0%
!! ACTTGTTGAGCATAATGTTT+TGG + chr7.4:20023773-20023792 MS.gene055718:intron 30.0%
!!! AGATTTAGCTCGTTTTTTAG+TGG - chr7.4:20024696-20024715 None:intergenic 30.0%
!!! ATAATGTTTTGGTAGGTTCA+TGG + chr7.4:20023784-20023803 MS.gene055718:intron 30.0%
AATGTCAAGCTACAATCCAA+GGG + chr7.4:20025036-20025055 MS.gene055718:CDS 35.0%
AGATTCACATGCTACAAAGA+AGG + chr7.4:20022835-20022854 MS.gene055718:CDS 35.0%
ATCATGTGAACATACTCTTC+CGG - chr7.4:20023015-20023034 None:intergenic 35.0%
ATGCATAGATCTACATGAAG+AGG + chr7.4:20023719-20023738 MS.gene055718:intron 35.0%
CACCACATACAAGACAAATA+AGG + chr7.4:20023386-20023405 MS.gene055718:CDS 35.0%
CATCATCAAGCTTCAATTCA+AGG + chr7.4:20024927-20024946 MS.gene055718:CDS 35.0%
CGCTTTAGTATATAGTGTTC+AGG - chr7.4:20024329-20024348 None:intergenic 35.0%
CTGTTGGCCAATTTAATCAT+TGG - chr7.4:20024458-20024477 None:intergenic 35.0%
CTTGAACAAAATGAGGAACA+TGG + chr7.4:20022652-20022671 MS.gene055718:CDS 35.0%
GATTTAAGGAAAAATGGGCA+TGG + chr7.4:20023823-20023842 MS.gene055718:intron 35.0%
GGAAGTAATCAAGATTGGAA+AGG + chr7.4:20024371-20024390 MS.gene055718:CDS 35.0%
GGATATGATATTTGAATCCG+AGG + chr7.4:20022708-20022727 MS.gene055718:CDS 35.0%
GGGTATTTCTTTAGTTATGC+CGG + chr7.4:20023593-20023612 MS.gene055718:CDS 35.0%
GTAAAAGAGAGGACAGTTAT+GGG + chr7.4:20024194-20024213 MS.gene055718:CDS 35.0%
GTAATTGAATTGAGGTCAAG+TGG - chr7.4:20024894-20024913 None:intergenic 35.0%
TATGTCAAACTGCAATCCAA+GGG + chr7.4:20024982-20025001 MS.gene055718:CDS 35.0%
TATGTTGGCCAAAAAATGTG+TGG + chr7.4:20024550-20024569 MS.gene055718:CDS 35.0%
TCAAATATCATATCCAAGCG+AGG - chr7.4:20022703-20022722 None:intergenic 35.0%
TCACTAAAGTATGGTGAAGT+GGG + chr7.4:20023210-20023229 MS.gene055718:CDS 35.0%
TCGAAAGATAGGGAAATACA+AGG + chr7.4:20022951-20022970 MS.gene055718:CDS 35.0%
TGAAATCCCATTTCATGAAG+CGG + chr7.4:20024414-20024433 MS.gene055718:CDS 35.0%
TGTAAAAGAGAGGACAGTTA+TGG + chr7.4:20024193-20024212 MS.gene055718:CDS 35.0%
TTGGCCAATTTAATCATTGG+AGG - chr7.4:20024455-20024474 None:intergenic 35.0%
TTGGTATTCGTCGAGAATAT+GGG + chr7.4:20022776-20022795 MS.gene055718:CDS 35.0%
TTGTCTACCAAAATAGACGA+CGG - chr7.4:20022604-20022623 None:intergenic 35.0%
TTTGCTTCGAACAAAAAGGA+TGG + chr7.4:20023185-20023204 MS.gene055718:CDS 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr7.4:20022775-20022794 MS.gene055718:CDS 35.0%
! ACCCTTATTTGTCTTGTATG+TGG - chr7.4:20023391-20023410 None:intergenic 35.0%
! CACTTTAAAAGAGCTGTCAA+TGG + chr7.4:20023963-20023982 MS.gene055718:intron 35.0%
! CATTTCATGGATTGTGTACT+TGG + chr7.4:20023619-20023638 MS.gene055718:CDS 35.0%
! GCCTTCTTCATTTTCAATCT+AGG - chr7.4:20024038-20024057 None:intergenic 35.0%
! TGATCCGTTAGAATGGTTTT+AGG - chr7.4:20023555-20023574 None:intergenic 35.0%
! TGTCGTAAATTTGAGACACA+CGG + chr7.4:20024248-20024267 MS.gene055718:CDS 35.0%
!! CAATCAAGCGAAGTTGATAA+TGG + chr7.4:20025008-20025027 MS.gene055718:CDS 35.0%
!! TAGTCATGCTTTGAAAGTGT+TGG + chr7.4:20024280-20024299 MS.gene055718:CDS 35.0%
!! TGCATAAAGGTGTTGAAAGA+AGG + chr7.4:20024143-20024162 MS.gene055718:CDS 35.0%
!!! CAAGAAACTTTTAGTTCCGT+AGG - chr7.4:20024224-20024243 None:intergenic 35.0%
!!! GTTGAGCATAATGTTTTGGT+AGG + chr7.4:20023777-20023796 MS.gene055718:intron 35.0%
!!! TTCTCCATCCACACATTTTT+TGG - chr7.4:20024561-20024580 None:intergenic 35.0%
!!! TTTTGGGGTGTAAACATTTC+CGG - chr7.4:20024078-20024097 None:intergenic 35.0%
AAAAACTTACCGCTAACGAC+TGG - chr7.4:20024790-20024809 None:intergenic 40.0%
AAAGAGCTGTCAATGGTAAG+CGG + chr7.4:20023970-20023989 MS.gene055718:intron 40.0%
AATGGGCATGGACTTATGTT+CGG + chr7.4:20023835-20023854 MS.gene055718:CDS 40.0%
ACTGTACCGCTTCATGAAAT+GGG - chr7.4:20024423-20024442 None:intergenic 40.0%
AGAATCAAGTGGAATGGTTC+AGG + chr7.4:20024577-20024596 MS.gene055718:CDS 40.0%
AGCATCTTGATCCGTTAGAA+TGG - chr7.4:20023562-20023581 None:intergenic 40.0%
AGTCCAACAAATACTCCCAA+CGG - chr7.4:20023420-20023439 None:intergenic 40.0%
ATGTCAAGCTACAATCCAAG+GGG + chr7.4:20025037-20025056 MS.gene055718:CDS 40.0%
CAATGTCAAGCTACAATCCA+AGG + chr7.4:20025035-20025054 MS.gene055718:CDS 40.0%
CACAATCCATGAAATGTCTC+CGG - chr7.4:20023615-20023634 None:intergenic 40.0%
CTATGTCAAACTGCAATCCA+AGG + chr7.4:20024981-20025000 MS.gene055718:CDS 40.0%
CTCACTAAAGTATGGTGAAG+TGG + chr7.4:20023209-20023228 MS.gene055718:CDS 40.0%
GAATATATGTCCAAGCAGGT+TGG + chr7.4:20023120-20023139 MS.gene055718:CDS 40.0%
GACAGTTATGGGAAATCCTA+CGG + chr7.4:20024205-20024224 MS.gene055718:CDS 40.0%
GATATTCCTCGTATTCCTCT+TGG - chr7.4:20024115-20024134 None:intergenic 40.0%
GCATGAATATATGTCCAAGC+AGG + chr7.4:20023116-20023135 MS.gene055718:CDS 40.0%
GCTGGACTCTTCATAAACTT+TGG - chr7.4:20024516-20024535 None:intergenic 40.0%
GGTTGGGAAGTAATCAAGAT+TGG + chr7.4:20024366-20024385 MS.gene055718:CDS 40.0%
TAAAGAGAAAGAAGCCAGCA+TGG + chr7.4:20024622-20024641 MS.gene055718:CDS 40.0%
TTGGCCAAAAAATGTGTGGA+TGG + chr7.4:20024554-20024573 MS.gene055718:CDS 40.0%
! ACTTTTCTCAAAGCAATGGG+TGG + chr7.4:20023522-20023541 MS.gene055718:CDS 40.0%
! AGGATGGACTCACTAAAGTA+TGG + chr7.4:20023201-20023220 MS.gene055718:CDS 40.0%
! TATGCCTTTTGCCTTGACAA+CGG - chr7.4:20024651-20024670 None:intergenic 40.0%
!! ATGAGATTCTGAAGCTCTGT+TGG - chr7.4:20024474-20024493 None:intergenic 40.0%
AAATAAGGGTTATCGGCCGT+TGG + chr7.4:20023401-20023420 MS.gene055718:CDS 45.0%
AATAAGGGTTATCGGCCGTT+GGG + chr7.4:20023402-20023421 MS.gene055718:CDS 45.0%
ACAAAAGACGTCAACCGACT+GGG + chr7.4:20022872-20022891 MS.gene055718:CDS 45.0%
ACAGGAAAACCAGTCGTTAG+CGG + chr7.4:20024778-20024797 MS.gene055718:CDS 45.0%
ACTAAAGCGATGGACAAGAG+AGG + chr7.4:20024340-20024359 MS.gene055718:CDS 45.0%
ATCCAAGCGAGGTTTGATGT+CGG - chr7.4:20022692-20022711 None:intergenic 45.0%
CACTGTACCGCTTCATGAAA+TGG - chr7.4:20024424-20024443 None:intergenic 45.0%
GAAACCGTTGTCAAGGCAAA+AGG + chr7.4:20024644-20024663 MS.gene055718:CDS 45.0%
GGAATTCCAAGAGGAATACG+AGG + chr7.4:20024106-20024125 MS.gene055718:CDS 45.0%
GGATGGAGAATCAAGTGGAA+TGG + chr7.4:20024571-20024590 MS.gene055718:CDS 45.0%
GTTATGCCGGAGACATTTCA+TGG + chr7.4:20023606-20023625 MS.gene055718:CDS 45.0%
GTTCAGGTAACCGTATCACT+TGG + chr7.4:20024593-20024612 MS.gene055718:CDS 45.0%
GTTCGGAAAACATTCACTGC+AGG + chr7.4:20023852-20023871 MS.gene055718:CDS 45.0%
TCTACGGCAGAAACTAGAAC+CGG + chr7.4:20022898-20022917 MS.gene055718:CDS 45.0%
TGTGTGGATGGAGAATCAAG+TGG + chr7.4:20024566-20024585 MS.gene055718:CDS 45.0%
! TATATGTCCAAGCAGGTTGG+TGG + chr7.4:20023123-20023142 MS.gene055718:CDS 45.0%
!! ATATGTCCAAGCAGGTTGGT+GGG + chr7.4:20023124-20023143 MS.gene055718:CDS 45.0%
AACCGGGTGTAATGCACGTA+TGG + chr7.4:20022915-20022934 MS.gene055718:CDS 50.0%
AACGGATCAAGATGCTGCGA+TGG + chr7.4:20023566-20023585 MS.gene055718:CDS 50.0%
ACACAACCACCTTCTTCAGC+CGG + chr7.4:20022993-20023012 MS.gene055718:CDS 50.0%
ACCCATACGTGCATTACACC+CGG - chr7.4:20022920-20022939 None:intergenic 50.0%
ACCGGGTGTAATGCACGTAT+GGG + chr7.4:20022916-20022935 MS.gene055718:CDS 50.0%
ATGGACAAGAGAGGCAAGGT+TGG + chr7.4:20024349-20024368 MS.gene055718:CDS 50.0%
CAAAAGACGTCAACCGACTG+GGG + chr7.4:20022873-20022892 MS.gene055718:CDS 50.0%
CAAGATGCTGCGATGGCAAA+GGG + chr7.4:20023573-20023592 MS.gene055718:CDS 50.0%
CAGCATGGAAACCGTTGTCA+AGG + chr7.4:20024637-20024656 MS.gene055718:CDS 50.0%
CATACTCTTCCGGCTGAAGA+AGG - chr7.4:20023005-20023024 None:intergenic 50.0%
CTACGGCAGAAACTAGAACC+GGG + chr7.4:20022899-20022918 MS.gene055718:CDS 50.0%
CTCTCATCGTCGCATATCTG+AGG + chr7.4:20023038-20023057 MS.gene055718:CDS 50.0%
CTTCGCTTGATTGTGTCCCT+TGG - chr7.4:20025001-20025020 None:intergenic 50.0%
CTTGACAACGGTTTCCATGC+TGG - chr7.4:20024639-20024658 None:intergenic 50.0%
GACAAAAGACGTCAACCGAC+TGG + chr7.4:20022871-20022890 MS.gene055718:CDS 50.0%
GGGTGTAAACATTTCCGGCT+TGG - chr7.4:20024073-20024092 None:intergenic 50.0%
TCAAGATGCTGCGATGGCAA+AGG + chr7.4:20023572-20023591 MS.gene055718:CDS 50.0%
TGGACAAGAGAGGCAAGGTT+GGG + chr7.4:20024350-20024369 MS.gene055718:CDS 50.0%
! GTTGGTGGGATAGAGATGGT+TGG + chr7.4:20023138-20023157 MS.gene055718:CDS 50.0%
ACTCTTCCGGCTGAAGAAGG+TGG - chr7.4:20023002-20023021 None:intergenic 55.0%
AGCGATGGACAAGAGAGGCA+AGG + chr7.4:20024345-20024364 MS.gene055718:CDS 55.0%
CTCTATCCCACCAACCTGCT+TGG - chr7.4:20023133-20023152 None:intergenic 55.0%
TCAACCGACTGGGGAGTCTA+CGG + chr7.4:20022882-20022901 MS.gene055718:CDS 55.0%
TGCCGACATCAAACCTCGCT+TGG + chr7.4:20022687-20022706 MS.gene055718:CDS 55.0%
!! CGGCCGTTGGGAGTATTTGT+TGG + chr7.4:20023414-20023433 MS.gene055718:CDS 55.0%
!! GCAGGTTGGTGGGATAGAGA+TGG + chr7.4:20023134-20023153 MS.gene055718:CDS 55.0%
GCTCGAATGCTCGTCCAAGC+CGG + chr7.4:20024056-20024075 MS.gene055718:CDS 60.0%
TCTGCCGTAGACTCCCCAGT+CGG - chr7.4:20022889-20022908 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 20022595 20025067 20022595 ID=MS.gene055718
chr7.4 mRNA 20022595 20025067 20022595 ID=MS.gene055718.t1;Parent=MS.gene055718
chr7.4 exon 20022595 20023690 20022595 ID=MS.gene055718.t1.exon1;Parent=MS.gene055718.t1
chr7.4 CDS 20022595 20023690 20022595 ID=cds.MS.gene055718.t1;Parent=MS.gene055718.t1
chr7.4 exon 20023831 20023873 20023831 ID=MS.gene055718.t1.exon2;Parent=MS.gene055718.t1
chr7.4 CDS 20023831 20023873 20023831 ID=cds.MS.gene055718.t1;Parent=MS.gene055718.t1
chr7.4 exon 20023974 20024799 20023974 ID=MS.gene055718.t1.exon3;Parent=MS.gene055718.t1
chr7.4 CDS 20023974 20024799 20023974 ID=cds.MS.gene055718.t1;Parent=MS.gene055718.t1
chr7.4 exon 20024888 20025067 20024888 ID=MS.gene055718.t1.exon4;Parent=MS.gene055718.t1
chr7.4 CDS 20024888 20025067 20024888 ID=cds.MS.gene055718.t1;Parent=MS.gene055718.t1
Gene Sequence

>MS.gene055718

ATGAATCCGTCGTCTATTTTGGTAGACAATTCTGTTGATAAATTGGTAAACAATATTCTTGAACAAAATGAGGAACATGGAAAAGAAAGTATTGCCGACATCAAACCTCGCTTGGATATGATATTTGAATCCGAGGTAGATGCTTATGAATTTTATAATGAGTATAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGGAATAAAAGCAAAAAAGACAGGGTTTTAACTTCAAGAAGATTCACATGCTACAAAGAAGGTACACGAAGTGTTGACAAAAGACGTCAACCGACTGGGGAGTCTACGGCAGAAACTAGAACCGGGTGTAATGCACGTATGGGTATTTCACTTGATCGAAAGATAGGGAAATACAAGGTTGTTGACTTTGTACTAGAACACAACCACCTTCTTCAGCCGGAAGAGTATGTTCACATGATTCGCTCTCATCGTCGCATATCTGAGGCACAAACATCACAAATTATAATGGGAGATGAATCTGGATTAAGACCAAAAGATTTGCATGAATATATGTCCAAGCAGGTTGGTGGGATAGAGATGGTTGGTTTTACAAGAACAAACGTTAAGAATTTGCTTCGAACAAAAAGGATGGACTCACTAAAGTATGGTGAAGTGGGTGCATTGATGACTTATTTCAAACAAGAAAGCGAAAATCCATCTTTCTTTTATGACTTTCAAATGGATGTTGAAGAGCAAATAACAAATATATTTTGGGCAGATGCACAAATGATCAATGATTATGGGTACTTTGGTGATGTCATCACTTTTGACACCACATACAAGACAAATAAGGGTTATCGGCCGTTGGGAGTATTTGTTGGACTTAATAATCATCGACAAACAATCATTTTTGGTGCAACATTGTTATATGATGAAACAATACCTTCTTTTCAGTGGTTATTTGAAACTTTTCTCAAAGCAATGGGTGGAAAAAAGCCTAAAACCATTCTAACGGATCAAGATGCTGCGATGGCAAAGGGTATTTCTTTAGTTATGCCGGAGACATTTCATGGATTGTGTACTTGGCATATAAGACAAAACGCTCTAAGGCATGTGAACCATCTATATCAAAAGAGTAAGCACTTTTCTTCAGATTTTGAAGCATGCATAGATCTACATGAAGAGGAAGATGAATTTTTGAATGCATGGAACTCTTTACTTGTTGAGCATAATGTTTTGGTAGGTTCATGGTTGCATATGATTTTTCGATTTAAGGAAAAATGGGCATGGACTTATGTTCGGAAAACATTCACTGCAGGTATGAGATCTACGCAATTAAGTGAAAGCTTTAATGCTGATTTGAAAAACCATTTGAAGTCAGATTTAAATATAGTACAATTTTTTACACACTTTAAAAGAGCTGTCAATGGTAAGCGGAACAATGAGTCAGAAGCTGAATATGAGTCAAGACATAAGCTACCTAGATTGAAAATGAAGAAGGCTCGAATGCTCGTCCAAGCCGGAAATGTTTACACCCCAAAAATATTTGAGGAATTCCAAGAGGAATACGAGGAATATCAAGACACTTGCATAAAGGTGTTGAAAGAAGGTTTGTATGCTGTCACAAATTATGATAATGTAAAAGAGAGGACAGTTATGGGAAATCCTACGGAACTAAAAGTTTCTTGTGATTGTCGTAAATTTGAGACACACGGCATCTTATGTAGTCATGCTTTGAAAGTGTTGGATGTCATGAATATTAAGTTAATTCCTGAACACTATATACTAAAGCGATGGACAAGAGAGGCAAGGTTGGGAAGTAATCAAGATTGGAAAGGGATGCATGTTGAACTAGACATGAAATCCCATTTCATGAAGCGGTACAGTGAGTTATGTCCTCCAATGATTAAATTGGCCAACAGAGCTTCAGAATCTCATGAAACTTACACTTTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAGTTGAAGATATGTTGGCCAAAAAATGTGTGGATGGAGAATCAAGTGGAATGGTTCAGGTAACCGTATCACTTGGAAATGATAAAGAGAAAGAAGCCAGCATGGAAACCGTTGTCAAGGCAAAAGGCATAAAGAAGCGAGATTGTTCACGTTCCACTAAAAAACGAGCTAAATCTTGGATTGAGAAAGAAGGTAAAAAGAGAAACAAATACACTCAGTCCCAAAAAAAGAGAAAATCACAGGAAAACCAGTCGTTAGCGGTAAGTTTTTTTCTTTCTTTCTTTACTTATCAATGTTTTAAATTTTGTTTAACTATATCATTCATTTCAGTTTTCTTATATTATGCAGCAACCACTTGACCTCAATTCAATTACTCTCAATGGAACTCATCATCAAGCTTCAATTCAAGGGACACAATCAAGTCAAGCTGACAATGTAATTCTATGTCAAACTGCAATCCAAGGGACACAATCAAGCGAAGTTGATAATGGAGCTCAATGTCAAGCTACAATCCAAGGGGCACAATGA
Protein sequence

>MS.gene055718.t1

MNPSSILVDNSVDKLVNNILEQNEEHGKESIADIKPRLDMIFESEVDAYEFYNEYSKRIGFGIRREYGNKSKKDRVLTSRRFTCYKEGTRSVDKRRQPTGESTAETRTGCNARMGISLDRKIGKYKVVDFVLEHNHLLQPEEYVHMIRSHRRISEAQTSQIIMGDESGLRPKDLHEYMSKQVGGIEMVGFTRTNVKNLLRTKRMDSLKYGEVGALMTYFKQESENPSFFYDFQMDVEEQITNIFWADAQMINDYGYFGDVITFDTTYKTNKGYRPLGVFVGLNNHRQTIIFGATLLYDETIPSFQWLFETFLKAMGGKKPKTILTDQDAAMAKGISLVMPETFHGLCTWHIRQNALRHVNHLYQKRKMGMDLCSENIHCRAVNGKRNNESEAEYESRHKLPRLKMKKARMLVQAGNVYTPKIFEEFQEEYEEYQDTCIKVLKEGLYAVTNYDNVKERTVMGNPTELKVSCDCRKFETHGILCSHALKVLDVMNIKLIPEHYILKRWTREARLGSNQDWKGMHVELDMKSHFMKRYSELCPPMIKLANRASESHETYTFLSKVYEESSKIVEDMLAKKCVDGESSGMVQVTVSLGNDKEKEASMETVVKAKGIKKRDCSRSTKKRAKSWIEKEGKKRNKYTQSQKKRKSQENQSLAQPLDLNSITLNGTHHQASIQGTQSSQADNVILCQTAIQGTQSSEVDNGAQCQATIQGAQ