Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055967.t1 | AES81523.1 | 95 | 161 | 8 | 0 | 1 | 161 | 1 | 161 | 1.60E-84 | 322 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055967.t1 | G7KWB3 | 95.0 | 161 | 8 | 0 | 1 | 161 | 1 | 161 | 1.1e-84 | 322.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene055967.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055967.t1 | MTR_7g093700 | 95.031 | 161 | 8 | 0 | 1 | 161 | 1 | 161 | 1.86e-104 | 296 |
| MS.gene055967.t1 | MTR_7g093670 | 84.146 | 82 | 13 | 0 | 80 | 161 | 1 | 82 | 2.65e-43 | 138 |
| MS.gene055967.t1 | MTR_7g093690 | 62.264 | 106 | 25 | 3 | 69 | 161 | 33 | 136 | 5.14e-33 | 114 |
| MS.gene055967.t1 | MTR_3g011210 | 49.451 | 91 | 22 | 2 | 72 | 138 | 31 | 121 | 2.95e-18 | 76.3 |
| MS.gene055967.t1 | MTR_7g093660 | 63.462 | 52 | 18 | 1 | 98 | 149 | 1 | 51 | 1.64e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATAATATCGATGGATCTT+TGG | 0.212883 | 7.4:-15326750 | MS.gene055967:CDS |
| AATAATATCGATGGATCTTT+GGG | 0.246908 | 7.4:-15326749 | MS.gene055967:CDS |
| GAACTGCACGAGATCTTTCT+TGG | 0.257265 | 7.4:-15326979 | MS.gene055967:CDS |
| ACACAATTTGCAACATAAAT+CGG | 0.291650 | 7.4:-15326943 | MS.gene055967:CDS |
| CAAATCAAGGTTGATGTCAT+TGG | 0.397831 | 7.4:+15326637 | None:intergenic |
| GTGCAGTTCTGAAGGTTTCA+TGG | 0.407889 | 7.4:+15326993 | None:intergenic |
| TGCAAATTGTGTATGTGCTT+TGG | 0.408651 | 7.4:+15326954 | None:intergenic |
| CACAATTTGCAACATAAATC+GGG | 0.419218 | 7.4:-15326942 | MS.gene055967:CDS |
| ACAATTGGAACCTCTGGTAT+AGG | 0.433089 | 7.4:+15326794 | None:intergenic |
| TGGCAATGGTTGCATGGAAT+AGG | 0.448209 | 7.4:+15327013 | None:intergenic |
| TTCTGAAGGTTTCATGGCAA+TGG | 0.457643 | 7.4:+15326999 | None:intergenic |
| TCAGATGATGAGAGCAGTAC+TGG | 0.465223 | 7.4:-15327061 | MS.gene055967:CDS |
| CGATCAGTACGAGAGACAAT+TGG | 0.466761 | 7.4:+15326779 | None:intergenic |
| TCAGTTGGAGCTGAATCAAT+CGG | 0.471657 | 7.4:+15326692 | None:intergenic |
| CAACCTAGAGCTTCAAATCA+AGG | 0.475468 | 7.4:+15326624 | None:intergenic |
| CGAGAGACAATTGGAACCTC+TGG | 0.486624 | 7.4:+15326788 | None:intergenic |
| TCTCATCATCTGATTCAGAT+AGG | 0.499444 | 7.4:+15327071 | None:intergenic |
| CTTCCATCCCGCCACACCAC+TGG | 0.504811 | 7.4:+15326662 | None:intergenic |
| TCTGGTATAGGCCTCATGTA+TGG | 0.510085 | 7.4:+15326806 | None:intergenic |
| ACCACTGGTAATTCTTCAGT+TGG | 0.510267 | 7.4:+15326677 | None:intergenic |
| CAACCTTGATTTGAAGCTCT+AGG | 0.512638 | 7.4:-15326627 | None:intergenic |
| TGTGATGAAGGTGGTGGAGA+TGG | 0.521845 | 7.4:+15326881 | None:intergenic |
| TAATTACCGCTTCCATGCAT+AGG | 0.528205 | 7.4:+15326845 | None:intergenic |
| ACTATGTTGTTTCCTATGCA+TGG | 0.528781 | 7.4:-15326857 | MS.gene055967:CDS |
| TTACCAGTGGTGTGGCGGGA+TGG | 0.529438 | 7.4:-15326665 | MS.gene055967:CDS |
| CGTGATTACAATAATATCGA+TGG | 0.538614 | 7.4:-15326758 | MS.gene055967:CDS |
| GTTTCATGGCAATGGTTGCA+TGG | 0.544580 | 7.4:+15327007 | None:intergenic |
| AAGAATTACCAGTGGTGTGG+CGG | 0.548905 | 7.4:-15326670 | MS.gene055967:CDS |
| GCCTCATGTATGGATGAAAA+CGG | 0.550640 | 7.4:+15326816 | None:intergenic |
| AAGATCTCGTGCAGTTCTGA+AGG | 0.551614 | 7.4:+15326985 | None:intergenic |
| TTGTTTCCTATGCATGGAAG+CGG | 0.563807 | 7.4:-15326851 | MS.gene055967:CDS |
| TGGATCTTTGGGGAGAACAA+TGG | 0.585871 | 7.4:-15326738 | MS.gene055967:CDS |
| AACAACATAGTGTGTGATGA+AGG | 0.595436 | 7.4:+15326869 | None:intergenic |
| AGAATTACCAGTGGTGTGGC+GGG | 0.597554 | 7.4:-15326669 | MS.gene055967:CDS |
| GATGAAGGTGGTGGAGATGG+TGG | 0.599841 | 7.4:+15326884 | None:intergenic |
| GTGGAGATGGTGGTCCAATG+TGG | 0.619599 | 7.4:+15326894 | None:intergenic |
| CTAAACAACACACACCACAT+TGG | 0.630539 | 7.4:-15326908 | MS.gene055967:CDS |
| CTGAAGAATTACCAGTGGTG+TGG | 0.651724 | 7.4:-15326673 | MS.gene055967:CDS |
| ATACATGAGGCCTATACCAG+AGG | 0.686494 | 7.4:-15326804 | MS.gene055967:CDS |
| TCCAACTGAAGAATTACCAG+TGG | 0.699300 | 7.4:-15326678 | MS.gene055967:CDS |
| ATAGTGTGTGATGAAGGTGG+TGG | 0.699659 | 7.4:+15326875 | None:intergenic |
| ATAATATCGATGGATCTTTG+GGG | 0.708326 | 7.4:-15326748 | MS.gene055967:CDS |
| AACATAGTGTGTGATGAAGG+TGG | 0.727614 | 7.4:+15326872 | None:intergenic |
| TCATGTATGGATGAAAACGG+CGG | 0.744847 | 7.4:+15326819 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAATATCGATGGATCTTT+GGG | - | chr7.4:15326970-15326989 | MS.gene055967:CDS | 25.0% |
| ! | ACACAATTTGCAACATAAAT+CGG | - | chr7.4:15326776-15326795 | MS.gene055967:CDS | 25.0% |
| ATAATATCGATGGATCTTTG+GGG | - | chr7.4:15326971-15326990 | MS.gene055967:CDS | 30.0% | |
| CAATAATATCGATGGATCTT+TGG | - | chr7.4:15326969-15326988 | MS.gene055967:CDS | 30.0% | |
| CACAATTTGCAACATAAATC+GGG | - | chr7.4:15326777-15326796 | MS.gene055967:CDS | 30.0% | |
| CGTGATTACAATAATATCGA+TGG | - | chr7.4:15326961-15326980 | MS.gene055967:CDS | 30.0% | |
| AACAACATAGTGTGTGATGA+AGG | + | chr7.4:15326853-15326872 | None:intergenic | 35.0% | |
| TCTCATCATCTGATTCAGAT+AGG | + | chr7.4:15326651-15326670 | None:intergenic | 35.0% | |
| TGCAAATTGTGTATGTGCTT+TGG | + | chr7.4:15326768-15326787 | None:intergenic | 35.0% | |
| !! | ACTATGTTGTTTCCTATGCA+TGG | - | chr7.4:15326862-15326881 | MS.gene055967:CDS | 35.0% |
| !! | CAAATCAAGGTTGATGTCAT+TGG | + | chr7.4:15327085-15327104 | None:intergenic | 35.0% |
| AACATAGTGTGTGATGAAGG+TGG | + | chr7.4:15326850-15326869 | None:intergenic | 40.0% | |
| ACAATTGGAACCTCTGGTAT+AGG | + | chr7.4:15326928-15326947 | None:intergenic | 40.0% | |
| ACCACTGGTAATTCTTCAGT+TGG | + | chr7.4:15327045-15327064 | None:intergenic | 40.0% | |
| CTAAACAACACACACCACAT+TGG | - | chr7.4:15326811-15326830 | MS.gene055967:CDS | 40.0% | |
| GCCTCATGTATGGATGAAAA+CGG | + | chr7.4:15326906-15326925 | None:intergenic | 40.0% | |
| TAATTACCGCTTCCATGCAT+AGG | + | chr7.4:15326877-15326896 | None:intergenic | 40.0% | |
| TCAGTTGGAGCTGAATCAAT+CGG | + | chr7.4:15327030-15327049 | None:intergenic | 40.0% | |
| TCATGTATGGATGAAAACGG+CGG | + | chr7.4:15326903-15326922 | None:intergenic | 40.0% | |
| TCCAACTGAAGAATTACCAG+TGG | - | chr7.4:15327041-15327060 | MS.gene055967:CDS | 40.0% | |
| TTGTTTCCTATGCATGGAAG+CGG | - | chr7.4:15326868-15326887 | MS.gene055967:CDS | 40.0% | |
| ! | TTCTGAAGGTTTCATGGCAA+TGG | + | chr7.4:15326723-15326742 | None:intergenic | 40.0% |
| AAGAATTACCAGTGGTGTGG+CGG | - | chr7.4:15327049-15327068 | MS.gene055967:CDS | 45.0% | |
| ATACATGAGGCCTATACCAG+AGG | - | chr7.4:15326915-15326934 | MS.gene055967:CDS | 45.0% | |
| ATAGTGTGTGATGAAGGTGG+TGG | + | chr7.4:15326847-15326866 | None:intergenic | 45.0% | |
| CGATCAGTACGAGAGACAAT+TGG | + | chr7.4:15326943-15326962 | None:intergenic | 45.0% | |
| CTGAAGAATTACCAGTGGTG+TGG | - | chr7.4:15327046-15327065 | MS.gene055967:CDS | 45.0% | |
| GAACTGCACGAGATCTTTCT+TGG | - | chr7.4:15326740-15326759 | MS.gene055967:CDS | 45.0% | |
| GTTTCATGGCAATGGTTGCA+TGG | + | chr7.4:15326715-15326734 | None:intergenic | 45.0% | |
| TCAGATGATGAGAGCAGTAC+TGG | - | chr7.4:15326658-15326677 | MS.gene055967:CDS | 45.0% | |
| TCTGGTATAGGCCTCATGTA+TGG | + | chr7.4:15326916-15326935 | None:intergenic | 45.0% | |
| TGGCAATGGTTGCATGGAAT+AGG | + | chr7.4:15326709-15326728 | None:intergenic | 45.0% | |
| ! | AAGATCTCGTGCAGTTCTGA+AGG | + | chr7.4:15326737-15326756 | None:intergenic | 45.0% |
| ! | TGGATCTTTGGGGAGAACAA+TGG | - | chr7.4:15326981-15327000 | MS.gene055967:CDS | 45.0% |
| !! | GCCGTTTTCATCCATACATG+AGG | - | chr7.4:15326902-15326921 | MS.gene055967:CDS | 45.0% |
| !! | GTGCAGTTCTGAAGGTTTCA+TGG | + | chr7.4:15326729-15326748 | None:intergenic | 45.0% |
| AGAATTACCAGTGGTGTGGC+GGG | - | chr7.4:15327050-15327069 | MS.gene055967:CDS | 50.0% | |
| CGAGAGACAATTGGAACCTC+TGG | + | chr7.4:15326934-15326953 | None:intergenic | 50.0% | |
| !! | TGTGATGAAGGTGGTGGAGA+TGG | + | chr7.4:15326841-15326860 | None:intergenic | 50.0% |
| ! | GTGGAGATGGTGGTCCAATG+TGG | + | chr7.4:15326828-15326847 | None:intergenic | 55.0% |
| !! | GATGAAGGTGGTGGAGATGG+TGG | + | chr7.4:15326838-15326857 | None:intergenic | 55.0% |
| ! | TTACCAGTGGTGTGGCGGGA+TGG | - | chr7.4:15327054-15327073 | MS.gene055967:CDS | 60.0% |
| CTTCCATCCCGCCACACCAC+TGG | + | chr7.4:15327060-15327079 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 15326628 | 15327113 | 15326628 | ID=MS.gene055967 |
| chr7.4 | mRNA | 15326628 | 15327113 | 15326628 | ID=MS.gene055967.t1;Parent=MS.gene055967 |
| chr7.4 | exon | 15326628 | 15327113 | 15326628 | ID=MS.gene055967.t1.exon1;Parent=MS.gene055967.t1 |
| chr7.4 | CDS | 15326628 | 15327113 | 15326628 | ID=cds.MS.gene055967.t1;Parent=MS.gene055967.t1 |
| Gene Sequence |
| Protein sequence |
