Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055969.t1 | AES81523.1 | 68.7 | 179 | 27 | 3 | 9 | 186 | 11 | 161 | 1.70E-58 | 235.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055969.t1 | G7KWB3 | 68.7 | 179 | 27 | 3 | 9 | 186 | 11 | 161 | 1.2e-58 | 235.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene055969.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055969.t1 | MTR_7g093700 | 66.667 | 189 | 32 | 4 | 1 | 186 | 1 | 161 | 2.38e-70 | 211 |
| MS.gene055969.t1 | MTR_7g093670 | 87.805 | 82 | 9 | 1 | 106 | 186 | 1 | 82 | 8.62e-46 | 145 |
| MS.gene055969.t1 | MTR_7g093690 | 54.545 | 132 | 43 | 5 | 70 | 186 | 7 | 136 | 4.73e-30 | 107 |
| MS.gene055969.t1 | MTR_3g011210 | 40.458 | 131 | 50 | 4 | 70 | 175 | 6 | 133 | 2.60e-17 | 74.7 |
| MS.gene055969.t1 | MTR_7g093660 | 53.247 | 77 | 22 | 2 | 123 | 186 | 1 | 76 | 4.77e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATAATATTGATGGATCTT+TGG | 0.189029 | 7.4:-15336180 | MS.gene055969:CDS |
| AATAATATTGATGGATCTTT+GGG | 0.232329 | 7.4:-15336179 | MS.gene055969:CDS |
| AAACTGCACAAGATCTTTCT+TGG | 0.245892 | 7.4:-15336460 | MS.gene055969:CDS |
| CGACCTTGATTTGAAGCTTT+AGG | 0.290526 | 7.4:-15336057 | None:intergenic |
| ACCATGTTGTTTCCTATTCA+TGG | 0.300234 | 7.4:-15336284 | MS.gene055969:CDS |
| ACACAATTTGCAACATAAAT+CGG | 0.316739 | 7.4:-15336424 | MS.gene055969:CDS |
| CATGATTACAATAATATTGA+TGG | 0.344856 | 7.4:-15336188 | MS.gene055969:CDS |
| TGTAAATCTTGTGATGTTGT+TGG | 0.389716 | 7.4:+15336359 | None:intergenic |
| CACAATTTGCAACATAAATC+GGG | 0.419218 | 7.4:-15336423 | MS.gene055969:CDS |
| TAATTACCGCTTCCATGAAT+AGG | 0.420026 | 7.4:+15336272 | None:intergenic |
| ACCGCTGGTACTTCTTCAAT+TGG | 0.421292 | 7.4:+15336107 | None:intergenic |
| TGTGATGAAGGTGGTGGAGA+TGG | 0.423532 | 7.4:+15336308 | None:intergenic |
| ACAATTGGAACCTCTGGTAT+AGG | 0.433089 | 7.4:+15336224 | None:intergenic |
| TGCAAATTGTGTATGAGCTT+TGG | 0.452975 | 7.4:+15336435 | None:intergenic |
| CAACCTAAAGCTTCAAATCA+AGG | 0.460309 | 7.4:+15336054 | None:intergenic |
| TGACGAGTACGAGAGACAAT+TGG | 0.479925 | 7.4:+15336209 | None:intergenic |
| TTGAAGAAGTACCAGCGGTC+TGG | 0.485006 | 7.4:-15336103 | MS.gene055969:CDS |
| CGAGAGACAATTGGAACCTC+TGG | 0.486624 | 7.4:+15336218 | None:intergenic |
| TCAATTGGAGCAGAATCAAT+CGG | 0.499372 | 7.4:+15336122 | None:intergenic |
| TTGTTTCCTATTCATGGAAG+CGG | 0.510636 | 7.4:-15336278 | MS.gene055969:CDS |
| TTTCCATCCTGCCAGACCGC+TGG | 0.517374 | 7.4:+15336092 | None:intergenic |
| AACCTCTGGTATAGGCCTCA+TGG | 0.524083 | 7.4:+15336232 | None:intergenic |
| AGAAGTACCAGCGGTCTGGC+AGG | 0.529373 | 7.4:-15336099 | MS.gene055969:CDS |
| GTTGGTATGAATGTTTGTGA+TGG | 0.529752 | 7.4:+15336377 | None:intergenic |
| GAGCAGAATCAATCGGCTTG+TGG | 0.540865 | 7.4:+15336129 | None:intergenic |
| AGATCTTCTTTGTACCACAT+TGG | 0.545928 | 7.4:-15336335 | MS.gene055969:CDS |
| AACAACATGGTGTGTGATGA+AGG | 0.556950 | 7.4:+15336296 | None:intergenic |
| GTACCAGCGGTCTGGCAGGA+TGG | 0.558642 | 7.4:-15336095 | MS.gene055969:CDS |
| TGGTTGCATCGAAATTCGAT+TGG | 0.568245 | 7.4:+15336500 | None:intergenic |
| TGGATCTTTGGGGAGAACAA+TGG | 0.571321 | 7.4:-15336168 | MS.gene055969:CDS |
| TAGGCCTCATGGATGGATGA+AGG | 0.586352 | 7.4:+15336243 | None:intergenic |
| GTTAGAGTAAGAACAAGAGT+AGG | 0.594186 | 7.4:+15336524 | None:intergenic |
| GATGAAGGTGGTGGAGATGG+TGG | 0.599841 | 7.4:+15336311 | None:intergenic |
| TCTGGTATAGGCCTCATGGA+TGG | 0.600026 | 7.4:+15336236 | None:intergenic |
| GTGGAGATGGTGGTCCAATG+TGG | 0.620516 | 7.4:+15336321 | None:intergenic |
| ATCCATGAGGCCTATACCAG+AGG | 0.624967 | 7.4:-15336234 | MS.gene055969:CDS |
| TCCAATTGAAGAAGTACCAG+CGG | 0.672889 | 7.4:-15336108 | MS.gene055969:CDS |
| ATAATATTGATGGATCTTTG+GGG | 0.673989 | 7.4:-15336178 | MS.gene055969:CDS |
| ATGGTGTGTGATGAAGGTGG+TGG | 0.684137 | 7.4:+15336302 | None:intergenic |
| GCCTCATGGATGGATGAAGG+TGG | 0.701559 | 7.4:+15336246 | None:intergenic |
| ACCACCTTCATCCATCCATG+AGG | 0.719012 | 7.4:-15336247 | MS.gene055969:CDS |
| TCCATGAATAGGAAACAACA+TGG | 0.720476 | 7.4:+15336283 | None:intergenic |
| AACATGGTGTGTGATGAAGG+TGG | 0.787924 | 7.4:+15336299 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATGATTACAATAATATTGA+TGG | - | chr7.4:15336466-15336485 | MS.gene055969:CDS | 20.0% |
| !!! | AATAATATTGATGGATCTTT+GGG | - | chr7.4:15336475-15336494 | MS.gene055969:CDS | 20.0% |
| ! | ACACAATTTGCAACATAAAT+CGG | - | chr7.4:15336230-15336249 | MS.gene055969:CDS | 25.0% |
| !! | ATAATATTGATGGATCTTTG+GGG | - | chr7.4:15336476-15336495 | MS.gene055969:CDS | 25.0% |
| !! | CAATAATATTGATGGATCTT+TGG | - | chr7.4:15336474-15336493 | MS.gene055969:CDS | 25.0% |
| CACAATTTGCAACATAAATC+GGG | - | chr7.4:15336231-15336250 | MS.gene055969:CDS | 30.0% | |
| TGTAAATCTTGTGATGTTGT+TGG | + | chr7.4:15336298-15336317 | None:intergenic | 30.0% | |
| AAACTGCACAAGATCTTTCT+TGG | - | chr7.4:15336194-15336213 | MS.gene055969:CDS | 35.0% | |
| AGATCTTCTTTGTACCACAT+TGG | - | chr7.4:15336319-15336338 | MS.gene055969:CDS | 35.0% | |
| GTTAGAGTAAGAACAAGAGT+AGG | + | chr7.4:15336133-15336152 | None:intergenic | 35.0% | |
| GTTGGTATGAATGTTTGTGA+TGG | + | chr7.4:15336280-15336299 | None:intergenic | 35.0% | |
| TAATTACCGCTTCCATGAAT+AGG | + | chr7.4:15336385-15336404 | None:intergenic | 35.0% | |
| TCAATTGGAGCAGAATCAAT+CGG | + | chr7.4:15336535-15336554 | None:intergenic | 35.0% | |
| TCCATGAATAGGAAACAACA+TGG | + | chr7.4:15336374-15336393 | None:intergenic | 35.0% | |
| TGCAAATTGTGTATGAGCTT+TGG | + | chr7.4:15336222-15336241 | None:intergenic | 35.0% | |
| TTGTTTCCTATTCATGGAAG+CGG | - | chr7.4:15336376-15336395 | MS.gene055969:CDS | 35.0% | |
| !! | AAGATCTTGTGCAGTTTTGA+AGG | + | chr7.4:15336191-15336210 | None:intergenic | 35.0% |
| !! | ACCATGTTGTTTCCTATTCA+TGG | - | chr7.4:15336370-15336389 | MS.gene055969:CDS | 35.0% |
| !!! | TTTTGAAGGTGTTATGGCAA+TGG | + | chr7.4:15336177-15336196 | None:intergenic | 35.0% |
| AACAACATGGTGTGTGATGA+AGG | + | chr7.4:15336361-15336380 | None:intergenic | 40.0% | |
| ACAATTGGAACCTCTGGTAT+AGG | + | chr7.4:15336433-15336452 | None:intergenic | 40.0% | |
| TGGTTGCATCGAAATTCGAT+TGG | + | chr7.4:15336157-15336176 | None:intergenic | 40.0% | |
| !! | TCCAATTGAAGAAGTACCAG+CGG | - | chr7.4:15336546-15336565 | MS.gene055969:CDS | 40.0% |
| !!! | GTGCAGTTTTGAAGGTGTTA+TGG | + | chr7.4:15336183-15336202 | None:intergenic | 40.0% |
| AACATGGTGTGTGATGAAGG+TGG | + | chr7.4:15336358-15336377 | None:intergenic | 45.0% | |
| ACCGCTGGTACTTCTTCAAT+TGG | + | chr7.4:15336550-15336569 | None:intergenic | 45.0% | |
| TGACGAGTACGAGAGACAAT+TGG | + | chr7.4:15336448-15336467 | None:intergenic | 45.0% | |
| ! | TGGATCTTTGGGGAGAACAA+TGG | - | chr7.4:15336486-15336505 | MS.gene055969:CDS | 45.0% |
| AACCTCTGGTATAGGCCTCA+TGG | + | chr7.4:15336425-15336444 | None:intergenic | 50.0% | |
| ACCACCTTCATCCATCCATG+AGG | - | chr7.4:15336407-15336426 | MS.gene055969:CDS | 50.0% | |
| ATCCATGAGGCCTATACCAG+AGG | - | chr7.4:15336420-15336439 | MS.gene055969:CDS | 50.0% | |
| ATGGTGTGTGATGAAGGTGG+TGG | + | chr7.4:15336355-15336374 | None:intergenic | 50.0% | |
| CGAGAGACAATTGGAACCTC+TGG | + | chr7.4:15336439-15336458 | None:intergenic | 50.0% | |
| TAGGCCTCATGGATGGATGA+AGG | + | chr7.4:15336414-15336433 | None:intergenic | 50.0% | |
| TCTGGTATAGGCCTCATGGA+TGG | + | chr7.4:15336421-15336440 | None:intergenic | 50.0% | |
| !! | GAGCAGAATCAATCGGCTTG+TGG | + | chr7.4:15336528-15336547 | None:intergenic | 50.0% |
| !! | TGTGATGAAGGTGGTGGAGA+TGG | + | chr7.4:15336349-15336368 | None:intergenic | 50.0% |
| !! | TTGAAGAAGTACCAGCGGTC+TGG | - | chr7.4:15336551-15336570 | MS.gene055969:CDS | 50.0% |
| GCCTCATGGATGGATGAAGG+TGG | + | chr7.4:15336411-15336430 | None:intergenic | 55.0% | |
| ! | GTGGAGATGGTGGTCCAATG+TGG | + | chr7.4:15336336-15336355 | None:intergenic | 55.0% |
| !! | GATGAAGGTGGTGGAGATGG+TGG | + | chr7.4:15336346-15336365 | None:intergenic | 55.0% |
| TTTCCATCCTGCCAGACCGC+TGG | + | chr7.4:15336565-15336584 | None:intergenic | 60.0% | |
| !! | AGAAGTACCAGCGGTCTGGC+AGG | - | chr7.4:15336555-15336574 | MS.gene055969:CDS | 60.0% |
| GTACCAGCGGTCTGGCAGGA+TGG | - | chr7.4:15336559-15336578 | MS.gene055969:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 15336058 | 15336618 | 15336058 | ID=MS.gene055969 |
| chr7.4 | mRNA | 15336058 | 15336618 | 15336058 | ID=MS.gene055969.t1;Parent=MS.gene055969 |
| chr7.4 | exon | 15336058 | 15336618 | 15336058 | ID=MS.gene055969.t1.exon1;Parent=MS.gene055969.t1 |
| chr7.4 | CDS | 15336058 | 15336618 | 15336058 | ID=cds.MS.gene055969.t1;Parent=MS.gene055969.t1 |
| Gene Sequence |
| Protein sequence |
