Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056154.t1 | XP_024632961.1 | 95.2 | 311 | 15 | 0 | 1 | 311 | 1 | 311 | 5.20E-153 | 550.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056154.t1 | Q9SZL8 | 28.8 | 257 | 165 | 6 | 52 | 308 | 74 | 312 | 3.6e-25 | 117.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056154.t1 | A0A072TE13 | 86.5 | 311 | 27 | 1 | 1 | 311 | 1 | 296 | 5.6e-149 | 536.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene056154.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056154.t1 | MTR_0558s0020 | 88.746 | 311 | 20 | 1 | 1 | 311 | 1 | 296 | 0.0 | 573 |
| MS.gene056154.t1 | MTR_1g083640 | 94.406 | 286 | 16 | 0 | 1 | 286 | 1 | 286 | 0.0 | 564 |
| MS.gene056154.t1 | MTR_8g446020 | 76.527 | 311 | 18 | 3 | 1 | 311 | 1 | 256 | 2.40e-167 | 465 |
| MS.gene056154.t1 | MTR_2g014100 | 86.561 | 253 | 25 | 2 | 1 | 252 | 1 | 245 | 6.70e-159 | 443 |
| MS.gene056154.t1 | MTR_6g090505 | 91.304 | 230 | 20 | 0 | 81 | 310 | 1 | 230 | 2.39e-157 | 444 |
| MS.gene056154.t1 | MTR_0337s0040 | 73.913 | 276 | 18 | 2 | 1 | 276 | 1 | 222 | 1.99e-139 | 393 |
| MS.gene056154.t1 | MTR_3g008240 | 84.404 | 218 | 19 | 1 | 1 | 218 | 1 | 203 | 5.49e-131 | 371 |
| MS.gene056154.t1 | MTR_4g040430 | 84.404 | 218 | 14 | 3 | 1 | 218 | 1 | 198 | 4.60e-128 | 363 |
| MS.gene056154.t1 | MTR_5g071590 | 87.432 | 183 | 20 | 1 | 1 | 183 | 1 | 180 | 1.17e-116 | 334 |
| MS.gene056154.t1 | MTR_0019s0290 | 89.474 | 152 | 16 | 0 | 98 | 249 | 9 | 160 | 2.75e-98 | 286 |
| MS.gene056154.t1 | MTR_1g055240 | 93.706 | 143 | 9 | 0 | 106 | 248 | 2 | 144 | 9.19e-98 | 285 |
| MS.gene056154.t1 | MTR_1281s0010 | 92.661 | 109 | 8 | 0 | 35 | 143 | 1 | 109 | 2.63e-69 | 211 |
| MS.gene056154.t1 | MTR_8g046060 | 77.083 | 96 | 13 | 2 | 1 | 95 | 91 | 178 | 2.71e-37 | 132 |
| MS.gene056154.t1 | MTR_4g008820 | 74.737 | 95 | 9 | 1 | 1 | 95 | 125 | 204 | 8.97e-36 | 136 |
| MS.gene056154.t1 | MTR_6g051425 | 31.250 | 256 | 147 | 6 | 56 | 304 | 22 | 255 | 1.00e-30 | 118 |
| MS.gene056154.t1 | MTR_1g070355 | 28.458 | 253 | 158 | 6 | 57 | 307 | 80 | 311 | 8.26e-22 | 95.9 |
| MS.gene056154.t1 | MTR_0337s0050 | 83.333 | 36 | 6 | 0 | 275 | 310 | 14 | 49 | 1.07e-15 | 71.2 |
| MS.gene056154.t1 | MTR_1g013060 | 29.032 | 217 | 133 | 8 | 56 | 269 | 29 | 227 | 1.42e-13 | 69.3 |
| MS.gene056154.t1 | MTR_0402s0040 | 27.419 | 186 | 114 | 5 | 65 | 249 | 40 | 205 | 2.30e-13 | 70.9 |
| MS.gene056154.t1 | MTR_4g035820 | 22.464 | 276 | 181 | 6 | 45 | 311 | 69 | 320 | 4.13e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056154.t1 | AT4G38180 | 29.008 | 262 | 158 | 7 | 52 | 308 | 74 | 312 | 2.56e-26 | 109 |
| MS.gene056154.t1 | AT5G28530 | 25.769 | 260 | 163 | 7 | 56 | 303 | 60 | 301 | 2.47e-15 | 77.0 |
| MS.gene056154.t1 | AT5G28530 | 25.769 | 260 | 163 | 7 | 56 | 303 | 60 | 301 | 2.81e-15 | 76.6 |
| MS.gene056154.t1 | AT5G28530 | 25.769 | 260 | 163 | 7 | 56 | 303 | 60 | 301 | 2.81e-15 | 76.6 |
| MS.gene056154.t1 | AT1G10240 | 22.846 | 267 | 186 | 7 | 48 | 311 | 45 | 294 | 1.20e-13 | 72.0 |
| MS.gene056154.t1 | AT1G10240 | 22.846 | 267 | 186 | 7 | 48 | 311 | 45 | 294 | 1.20e-13 | 72.0 |
Find 37 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATGTTGTCCTGTTGTT+AGG | 0.288908 | 1.2:+49356205 | None:intergenic |
| GGTAATTATGCATATAAAAC+TGG | 0.302948 | 1.2:-49356349 | MS.gene056154:CDS |
| TTACTTCTCGAAATGATCTA+AGG | 0.311482 | 1.2:+49356014 | None:intergenic |
| CAACTAGATTATTCTCTATT+CGG | 0.323831 | 1.2:-49355698 | MS.gene056154:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.341758 | 1.2:+49355648 | None:intergenic |
| AACAATAAATATCGGCCATT+AGG | 0.388497 | 1.2:-49355641 | MS.gene056154:CDS |
| ACCGAACAAACAATAAATAT+CGG | 0.389395 | 1.2:-49355649 | MS.gene056154:CDS |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 1.2:-49355939 | MS.gene056154:CDS |
| ATGTGTATAAGATATATAGA+TGG | 0.407680 | 1.2:-49356084 | MS.gene056154:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 1.2:-49355926 | MS.gene056154:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 1.2:-49356001 | MS.gene056154:CDS |
| ACAAAATAAGAAATTTGATA+AGG | 0.431852 | 1.2:-49356287 | MS.gene056154:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 1.2:-49356136 | MS.gene056154:CDS |
| GCTCTTATAAATTCTAAAGC+TGG | 0.462891 | 1.2:-49355971 | MS.gene056154:CDS |
| TCCATGATACTTTATTGCTA+GGG | 0.464004 | 1.2:+49356416 | None:intergenic |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 1.2:-49355938 | MS.gene056154:CDS |
| GTTTGCTTAATCCTAACAAC+AGG | 0.467124 | 1.2:-49356216 | MS.gene056154:CDS |
| TTTAAGTCTGAAAACCGAAA+AGG | 0.483010 | 1.2:+49355887 | None:intergenic |
| TTCCATGATACTTTATTGCT+AGG | 0.489989 | 1.2:+49356415 | None:intergenic |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 1.2:-49356253 | MS.gene056154:CDS |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | 1.2:-49356119 | MS.gene056154:CDS |
| CCCCTAGCAATAAAGTATCA+TGG | 0.498381 | 1.2:-49356417 | MS.gene056154:CDS |
| CTAAGGTAACATGAATGCTC+TGG | 0.504903 | 1.2:+49356031 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 1.2:-49356242 | MS.gene056154:CDS |
| GAATGCTCTGGTTTATGTAA+TGG | 0.517705 | 1.2:+49356043 | None:intergenic |
| TTACAACCAACCCTTTCCTC+CGG | 0.518116 | 1.2:+49356142 | None:intergenic |
| TGTCTTCTGTAGCATCAACT+TGG | 0.520245 | 1.2:+49355753 | None:intergenic |
| TGTGTATAAGATATATAGAT+GGG | 0.523615 | 1.2:-49356083 | MS.gene056154:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 1.2:-49356241 | MS.gene056154:CDS |
| TTCACAAACTCAATAACCAA+TGG | 0.554620 | 1.2:+49356178 | None:intergenic |
| GGAGTAACATTGAGAACTAT+CGG | 0.556951 | 1.2:+49356438 | None:intergenic |
| CCATGATACTTTATTGCTAG+GGG | 0.563054 | 1.2:+49356417 | None:intergenic |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 1.2:-49355923 | MS.gene056154:CDS |
| TTACTGATTCATGCTGAGAG+TGG | 0.579535 | 1.2:-49356496 | MS.gene056154:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 1.2:-49355932 | MS.gene056154:CDS |
| GACAACATCAGACAGTCCAT+TGG | 0.604607 | 1.2:-49356194 | MS.gene056154:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 1.2:-49356541 | MS.gene056154:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATTTGATA+AGG | - | chr1.2:49355900-49355919 | MS.gene056154:CDS | 15.0% |
| !! | ATGTGTATAAGATATATAGA+TGG | - | chr1.2:49356103-49356122 | MS.gene056154:CDS | 20.0% |
| !! | TGTGTATAAGATATATAGAT+GGG | - | chr1.2:49356104-49356123 | MS.gene056154:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | - | chr1.2:49355817-49355836 | MS.gene056154:CDS | 20.0% |
| !!! | ATAAAACTGGATTTTCTATT+AGG | - | chr1.2:49355851-49355870 | MS.gene056154:CDS | 20.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | chr1.2:49356538-49356557 | MS.gene056154:CDS | 25.0% |
| ! | CAACTAGATTATTCTCTATT+CGG | - | chr1.2:49356489-49356508 | MS.gene056154:CDS | 25.0% |
| ! | GGTAATTATGCATATAAAAC+TGG | - | chr1.2:49355838-49355857 | MS.gene056154:CDS | 25.0% |
| ! | TGGATATCATAGTAAAAAGA+TGG | + | chr1.2:49356417-49356436 | None:intergenic | 25.0% |
| !!! | AAGACATAGCTAGTATTTTT+TGG | - | chr1.2:49356451-49356470 | MS.gene056154:CDS | 25.0% |
| !!! | AGACATAGCTAGTATTTTTT+GGG | - | chr1.2:49356452-49356471 | MS.gene056154:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | - | chr1.2:49355946-49355965 | MS.gene056154:CDS | 30.0% | |
| AACAATAAATATCGGCCATT+AGG | - | chr1.2:49356546-49356565 | MS.gene056154:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr1.2:49356249-49356268 | MS.gene056154:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | chr1.2:49355934-49355953 | MS.gene056154:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | chr1.2:49356542-49356561 | None:intergenic | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | chr1.2:49355945-49355964 | MS.gene056154:CDS | 30.0% | |
| TCCATGATACTTTATTGCTA+GGG | + | chr1.2:49355774-49355793 | None:intergenic | 30.0% | |
| TGTGAAAGTGAAAGAAAAAC+CGG | - | chr1.2:49356026-49356045 | MS.gene056154:CDS | 30.0% | |
| TTACTTCTCGAAATGATCTA+AGG | + | chr1.2:49356176-49356195 | None:intergenic | 30.0% | |
| TTCACAAACTCAATAACCAA+TGG | + | chr1.2:49356012-49356031 | None:intergenic | 30.0% | |
| TTCCATGATACTTTATTGCT+AGG | + | chr1.2:49355775-49355794 | None:intergenic | 30.0% | |
| TTTAAGTCTGAAAACCGAAA+AGG | + | chr1.2:49356303-49356322 | None:intergenic | 30.0% | |
| !! | GCTCTTATAAATTCTAAAGC+TGG | - | chr1.2:49356216-49356235 | MS.gene056154:CDS | 30.0% |
| AATTCGTCAAAAAGAGATGG+TGG | - | chr1.2:49356338-49356357 | MS.gene056154:CDS | 35.0% | |
| ATATCAACTTCATCTGAGAG+TGG | - | chr1.2:49355646-49355665 | MS.gene056154:CDS | 35.0% | |
| CCATGATACTTTATTGCTAG+GGG | + | chr1.2:49355773-49355792 | None:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr1.2:49356186-49356205 | MS.gene056154:CDS | 35.0% | |
| GACAATTCGTCAAAAAGAGA+TGG | - | chr1.2:49356335-49356354 | MS.gene056154:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr1.2:49356248-49356267 | MS.gene056154:CDS | 35.0% | |
| GGAGTAACATTGAGAACTAT+CGG | + | chr1.2:49355752-49355771 | None:intergenic | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | chr1.2:49355971-49355990 | MS.gene056154:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | chr1.2:49356255-49356274 | MS.gene056154:CDS | 35.0% | |
| ! | GAATGCTCTGGTTTATGTAA+TGG | + | chr1.2:49356147-49356166 | None:intergenic | 35.0% |
| CTAAGGTAACATGAATGCTC+TGG | + | chr1.2:49356159-49356178 | None:intergenic | 40.0% | |
| GAAAGAAAAACCGGAGGAAA+GGG | - | chr1.2:49356035-49356054 | MS.gene056154:CDS | 40.0% | |
| GAAAGTGAAAGAAAAACCGG+AGG | - | chr1.2:49356029-49356048 | MS.gene056154:CDS | 40.0% | |
| TCTGATGTTGTCCTGTTGTT+AGG | + | chr1.2:49355985-49356004 | None:intergenic | 40.0% | |
| TGAAAGAAAAACCGGAGGAA+AGG | - | chr1.2:49356034-49356053 | MS.gene056154:CDS | 40.0% | |
| TGTCTTCTGTAGCATCAACT+TGG | + | chr1.2:49356437-49356456 | None:intergenic | 40.0% | |
| ! | CCCCTAGCAATAAAGTATCA+TGG | - | chr1.2:49355770-49355789 | MS.gene056154:CDS | 40.0% |
| ! | GCACGAAGAATCTTCCTTTT+CGG | - | chr1.2:49356286-49356305 | MS.gene056154:CDS | 40.0% |
| !! | AGCTGGAATATCTTTGAAGC+TGG | - | chr1.2:49356068-49356087 | MS.gene056154:CDS | 40.0% |
| !! | TTACTGATTCATGCTGAGAG+TGG | - | chr1.2:49355691-49355710 | MS.gene056154:CDS | 40.0% |
| !!! | GCTAGTATTTTTTGGGCAGA+TGG | - | chr1.2:49356459-49356478 | MS.gene056154:CDS | 40.0% |
| GAAGTTATGGGTCAAGGAGT+TGG | - | chr1.2:49356261-49356280 | MS.gene056154:CDS | 45.0% | |
| GACAACATCAGACAGTCCAT+TGG | - | chr1.2:49355993-49356012 | MS.gene056154:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+CGG | + | chr1.2:49356048-49356067 | None:intergenic | 45.0% | |
| ! | CGTCAAAAAGAGATGGTGGT+TGG | - | chr1.2:49356342-49356361 | MS.gene056154:CDS | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr1.2:49356051-49356070 | MS.gene056154:CDS | 45.0% |
| GAAAAACCGGAGGAAAGGGT+TGG | - | chr1.2:49356039-49356058 | MS.gene056154:CDS | 50.0% | |
| GTTATGGGTCAAGGAGTTGG+AGG | - | chr1.2:49356264-49356283 | MS.gene056154:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 49355637 | 49356572 | 49355637 | ID=MS.gene056154 |
| chr1.2 | mRNA | 49355637 | 49356572 | 49355637 | ID=MS.gene056154.t1;Parent=MS.gene056154 |
| chr1.2 | exon | 49355637 | 49356572 | 49355637 | ID=MS.gene056154.t1.exon1;Parent=MS.gene056154.t1 |
| chr1.2 | CDS | 49355637 | 49356572 | 49355637 | ID=cds.MS.gene056154.t1;Parent=MS.gene056154.t1 |
| Gene Sequence |
| Protein sequence |
