Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056225.t1 | XP_003627814.1 | 98.3 | 118 | 2 | 0 | 1 | 118 | 1 | 118 | 1.70E-56 | 228.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056225.t1 | O80366 | 68.6 | 121 | 34 | 2 | 1 | 118 | 1 | 120 | 4.5e-37 | 155.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056225.t1 | Q1RU46 | 98.3 | 118 | 2 | 0 | 1 | 118 | 1 | 118 | 1.2e-56 | 228.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene056225.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056225.t1 | MTR_8g038620 | 98.305 | 118 | 2 | 0 | 1 | 118 | 1 | 118 | 1.26e-82 | 239 |
| MS.gene056225.t1 | MTR_7g490310 | 75.630 | 119 | 28 | 1 | 1 | 118 | 1 | 119 | 3.34e-59 | 182 |
| MS.gene056225.t1 | MTR_3g015490 | 81.132 | 106 | 19 | 1 | 8 | 112 | 3 | 108 | 5.57e-57 | 174 |
| MS.gene056225.t1 | MTR_6g007460 | 60.000 | 155 | 14 | 2 | 3 | 117 | 5 | 151 | 7.88e-53 | 165 |
| MS.gene056225.t1 | MTR_3g088630 | 56.557 | 122 | 42 | 2 | 6 | 117 | 19 | 139 | 4.42e-41 | 136 |
| MS.gene056225.t1 | MTR_5g036480 | 59.292 | 113 | 40 | 2 | 7 | 118 | 14 | 121 | 2.49e-38 | 129 |
| MS.gene056225.t1 | MTR_1g049100 | 60.714 | 112 | 38 | 2 | 7 | 118 | 6 | 111 | 6.92e-38 | 127 |
| MS.gene056225.t1 | MTR_3g093860 | 57.798 | 109 | 40 | 2 | 10 | 118 | 19 | 121 | 7.93e-38 | 125 |
| MS.gene056225.t1 | MTR_3g078613 | 54.783 | 115 | 42 | 2 | 4 | 117 | 18 | 123 | 2.88e-35 | 120 |
| MS.gene056225.t1 | MTR_4g106590 | 57.265 | 117 | 39 | 3 | 4 | 118 | 21 | 128 | 5.41e-35 | 120 |
| MS.gene056225.t1 | MTR_3g078613 | 54.310 | 116 | 42 | 3 | 4 | 117 | 18 | 124 | 1.94e-33 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056225.t1 | AT3G57040 | 68.595 | 121 | 34 | 2 | 1 | 118 | 1 | 120 | 7.38e-52 | 163 |
| MS.gene056225.t1 | AT3G57040 | 68.595 | 121 | 34 | 2 | 1 | 118 | 1 | 120 | 7.38e-52 | 163 |
| MS.gene056225.t1 | AT2G41310 | 64.407 | 118 | 42 | 0 | 1 | 118 | 1 | 118 | 2.66e-50 | 159 |
| MS.gene056225.t1 | AT1G59940 | 60.714 | 112 | 36 | 2 | 7 | 118 | 31 | 134 | 2.72e-40 | 134 |
| MS.gene056225.t1 | AT3G56380 | 53.043 | 115 | 47 | 1 | 4 | 118 | 15 | 122 | 9.36e-40 | 130 |
| MS.gene056225.t1 | AT1G59940 | 60.714 | 112 | 36 | 2 | 7 | 118 | 123 | 226 | 7.66e-39 | 132 |
| MS.gene056225.t1 | AT2G40670 | 52.212 | 113 | 49 | 1 | 6 | 118 | 26 | 133 | 2.27e-38 | 127 |
| MS.gene056225.t1 | AT3G48100 | 55.085 | 118 | 44 | 2 | 2 | 118 | 18 | 127 | 2.38e-38 | 127 |
| MS.gene056225.t1 | AT1G10470 | 58.036 | 112 | 39 | 1 | 7 | 118 | 18 | 121 | 4.82e-38 | 128 |
| MS.gene056225.t1 | AT1G10470 | 58.036 | 112 | 39 | 1 | 7 | 118 | 32 | 135 | 5.49e-38 | 129 |
| MS.gene056225.t1 | AT3G56380 | 52.586 | 116 | 47 | 2 | 4 | 118 | 15 | 123 | 6.03e-38 | 125 |
| MS.gene056225.t1 | AT5G62920 | 55.652 | 115 | 43 | 1 | 4 | 118 | 20 | 126 | 1.33e-37 | 125 |
| MS.gene056225.t1 | AT1G19050 | 55.455 | 110 | 41 | 1 | 8 | 117 | 23 | 124 | 3.96e-37 | 125 |
| MS.gene056225.t1 | AT2G40670 | 51.754 | 114 | 49 | 2 | 6 | 118 | 26 | 134 | 1.28e-36 | 122 |
| MS.gene056225.t1 | AT1G74890 | 54.955 | 111 | 42 | 1 | 8 | 118 | 17 | 119 | 1.97e-36 | 123 |
Find 20 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAAGGCTTTAAAATTTCT+TGG | 0.266953 | 8.1:+65204362 | MS.gene056225:CDS |
| AGGTATGTCTTTGAAAGATT+TGG | 0.295699 | 8.1:-65205076 | None:intergenic |
| AGAGAATCAGTTCCATGTTT+TGG | 0.307421 | 8.1:+65204105 | MS.gene056225:CDS |
| GGCTTTAAAATTTCTTGGTT+TGG | 0.308229 | 8.1:+65204367 | MS.gene056225:CDS |
| TCTCACCTATTGATCCTAGA+TGG | 0.331221 | 8.1:-65205126 | None:intergenic |
| TACAGTTGATTCTGCTAGTA+AGG | 0.354062 | 8.1:+65204346 | MS.gene056225:CDS |
| TGTTGCTTCAGAAATTAATC+AGG | 0.404529 | 8.1:+65204421 | MS.gene056225:CDS |
| ATGTTCCATCTAGGATCAAT+AGG | 0.432186 | 8.1:+65205121 | MS.gene056225:CDS |
| GTTTGGTTGAGGATGAAATC+AGG | 0.441138 | 8.1:+65204384 | MS.gene056225:CDS |
| AAAATTTCTTGGTTTGGTTG+AGG | 0.449963 | 8.1:+65204373 | MS.gene056225:CDS |
| ATAACAGATTATTGTATGCC+AGG | 0.463952 | 8.1:+65204555 | MS.gene056225:CDS |
| TCTGAGGACACTATCACAAC+AGG | 0.494621 | 8.1:-65205096 | None:intergenic |
| TATTGTATGCCAGGGATGAC+CGG | 0.500365 | 8.1:+65204564 | MS.gene056225:CDS |
| TTGATAGAATGTTGATTGAG+AGG | 0.516155 | 8.1:+65204146 | MS.gene056225:CDS |
| AGATCATAGCCGGTCATCCC+TGG | 0.522997 | 8.1:-65204573 | None:intergenic |
| CCTCAGAGAATGTTCCATCT+AGG | 0.527247 | 8.1:+65205112 | MS.gene056225:CDS |
| TAACAGATTATTGTATGCCA+GGG | 0.564090 | 8.1:+65204556 | MS.gene056225:CDS |
| TATCATCAACAGCCAAAACA+TGG | 0.580138 | 8.1:-65204117 | None:intergenic |
| TATCTTTCAAGTAATGGGCA+TGG | 0.581553 | 8.1:+65204078 | None:intergenic |
| CCTAGATGGAACATTCTCTG+AGG | 0.707424 | 8.1:-65205112 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACAAATTTATCTAATAAAA+TGG | - | chr8.1:65204486-65204505 | None:intergenic | 10.0% |
| !! | TATTGAATAGATAAATATTA+TGG | + | chr8.1:65204454-65204473 | MS.gene056225:intron | 10.0% |
| !!! | AGATTAGTTAATTGAAAATT+TGG | - | chr8.1:65204924-65204943 | None:intergenic | 15.0% |
| !!! | ATTATTTTGCTTTTCTATTT+AGG | + | chr8.1:65204757-65204776 | MS.gene056225:intron | 15.0% |
| !!! | GATTAGTTAATTGAAAATTT+GGG | - | chr8.1:65204923-65204942 | None:intergenic | 15.0% |
| !! | TAGGAAACAATTTGAAAAAT+AGG | + | chr8.1:65204776-65204795 | MS.gene056225:intron | 20.0% |
| !! | TTTGATTAAATTATTCATGC+AGG | + | chr8.1:65204512-65204531 | MS.gene056225:intron | 20.0% |
| !!! | GATCTTATTTCTTAAATACA+TGG | + | chr8.1:65204798-65204817 | MS.gene056225:intron | 20.0% |
| ! | ATCAATTCTCACAATTGATT+TGG | + | chr8.1:65204890-65204909 | MS.gene056225:intron | 25.0% |
| ! | TAGACTAATCAAATAAGAAG+TGG | - | chr8.1:65204661-65204680 | None:intergenic | 25.0% |
| ! | TTGAATGCATCAAAAAAGTT+TGG | + | chr8.1:65204730-65204749 | MS.gene056225:intron | 25.0% |
| !! | CATTAAAAGAAGAAGTTTTG+AGG | - | chr8.1:65204174-65204193 | None:intergenic | 25.0% |
| !! | CTCAAAACTTCTTCTTTTAA+TGG | + | chr8.1:65204172-65204191 | MS.gene056225:CDS | 25.0% |
| !!! | AGTAAGGCTTTAAAATTTCT+TGG | + | chr8.1:65204362-65204381 | MS.gene056225:CDS | 25.0% |
| AATAAAGTTCATTGATCTGC+AGG | + | chr8.1:65205050-65205069 | MS.gene056225:intron | 30.0% | |
| ATAACAGATTATTGTATGCC+AGG | + | chr8.1:65204555-65204574 | MS.gene056225:CDS | 30.0% | |
| CAAAAGTAAGCACAAAATTG+TGG | - | chr8.1:65204706-65204725 | None:intergenic | 30.0% | |
| TAACAGATTATTGTATGCCA+GGG | + | chr8.1:65204556-65204575 | MS.gene056225:CDS | 30.0% | |
| TCTCAATCCAAAATTGTTCA+TGG | + | chr8.1:65204941-65204960 | MS.gene056225:intron | 30.0% | |
| TGATCTGTTGAGAAAAATCA+AGG | + | chr8.1:65204590-65204609 | MS.gene056225:CDS | 30.0% | |
| ! | AGGTATGTCTTTGAAAGATT+TGG | - | chr8.1:65205079-65205098 | None:intergenic | 30.0% |
| ! | ATGCTTTCCATGAACAATTT+TGG | - | chr8.1:65204951-65204970 | None:intergenic | 30.0% |
| ! | TGTTGCTTCAGAAATTAATC+AGG | + | chr8.1:65204421-65204440 | MS.gene056225:CDS | 30.0% |
| ! | TTGATAGAATGTTGATTGAG+AGG | + | chr8.1:65204146-65204165 | MS.gene056225:CDS | 30.0% |
| !! | AAAATTTCTTGGTTTGGTTG+AGG | + | chr8.1:65204373-65204392 | MS.gene056225:CDS | 30.0% |
| !! | GGCTTTAAAATTTCTTGGTT+TGG | + | chr8.1:65204367-65204386 | MS.gene056225:CDS | 30.0% |
| CTGTTGAGAAAAATCAAGGT+AGG | + | chr8.1:65204594-65204613 | MS.gene056225:intron | 35.0% | |
| TATCATCAACAGCCAAAACA+TGG | - | chr8.1:65204120-65204139 | None:intergenic | 35.0% | |
| ! | AGAGAATCAGTTCCATGTTT+TGG | + | chr8.1:65204105-65204124 | MS.gene056225:CDS | 35.0% |
| ! | ATGTTCCATCTAGGATCAAT+AGG | + | chr8.1:65205121-65205140 | MS.gene056225:CDS | 35.0% |
| ! | TACAGTTGATTCTGCTAGTA+AGG | + | chr8.1:65204346-65204365 | MS.gene056225:CDS | 35.0% |
| ! | TTTTCTCAACAGATCATAGC+CGG | - | chr8.1:65204586-65204605 | None:intergenic | 35.0% |
| GTTTGGTTGAGGATGAAATC+AGG | + | chr8.1:65204384-65204403 | MS.gene056225:CDS | 40.0% | |
| CCTAGATGGAACATTCTCTG+AGG | - | chr8.1:65205115-65205134 | None:intergenic | 45.0% | |
| CCTCAGAGAATGTTCCATCT+AGG | + | chr8.1:65205112-65205131 | MS.gene056225:CDS | 45.0% | |
| TATTGTATGCCAGGGATGAC+CGG | + | chr8.1:65204564-65204583 | MS.gene056225:CDS | 45.0% | |
| TCTGAGGACACTATCACAAC+AGG | - | chr8.1:65205099-65205118 | None:intergenic | 45.0% | |
| AGATCATAGCCGGTCATCCC+TGG | - | chr8.1:65204576-65204595 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 65204091 | 65205146 | 65204091 | ID=MS.gene056225 |
| chr8.1 | mRNA | 65204091 | 65205146 | 65204091 | ID=MS.gene056225.t1;Parent=MS.gene056225 |
| chr8.1 | exon | 65204091 | 65204193 | 65204091 | ID=MS.gene056225.t1.exon1;Parent=MS.gene056225.t1 |
| chr8.1 | CDS | 65204091 | 65204193 | 65204091 | ID=cds.MS.gene056225.t1;Parent=MS.gene056225.t1 |
| chr8.1 | exon | 65204342 | 65204442 | 65204342 | ID=MS.gene056225.t1.exon2;Parent=MS.gene056225.t1 |
| chr8.1 | CDS | 65204342 | 65204442 | 65204342 | ID=cds.MS.gene056225.t1;Parent=MS.gene056225.t1 |
| chr8.1 | exon | 65204534 | 65204611 | 65204534 | ID=MS.gene056225.t1.exon3;Parent=MS.gene056225.t1 |
| chr8.1 | CDS | 65204534 | 65204611 | 65204534 | ID=cds.MS.gene056225.t1;Parent=MS.gene056225.t1 |
| chr8.1 | exon | 65205072 | 65205146 | 65205072 | ID=MS.gene056225.t1.exon4;Parent=MS.gene056225.t1 |
| chr8.1 | CDS | 65205072 | 65205146 | 65205072 | ID=cds.MS.gene056225.t1;Parent=MS.gene056225.t1 |
| Gene Sequence |
| Protein sequence |
