AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056355

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056355.t1 MTR_4g066380 84.877 324 30 4 1 306 1 323 0.0 515
MS.gene056355.t1 MTR_4g066460 79.941 339 28 7 1 309 1 329 3.49e-178 495
MS.gene056355.t1 MTR_4g067010 76.877 333 35 6 1 306 1 318 2.93e-174 485
MS.gene056355.t1 MTR_6g047570 61.424 337 101 7 1 309 1 336 8.24e-131 375
MS.gene056355.t1 MTR_0011s0260 56.216 370 89 8 1 308 1 359 4.70e-115 336
MS.gene056355.t1 MTR_0011s0210 56.334 371 89 8 1 309 1 360 1.45e-114 335
MS.gene056355.t1 MTR_0250s0040 57.718 298 82 8 23 309 1 265 3.31e-103 303
MS.gene056355.t1 MTR_4g098035 49.133 346 135 12 1 309 1 342 1.67e-86 263
MS.gene056355.t1 MTR_0246s0020 45.170 383 132 11 1 309 1 379 7.70e-85 260
MS.gene056355.t1 MTR_0246s0050 48.598 321 125 11 25 309 5 321 1.55e-78 242
MS.gene056355.t1 MTR_0246s0020 42.382 361 130 11 1 287 1 357 7.14e-70 221
MS.gene056355.t1 MTR_6g047550 53.509 228 74 6 1 196 1 228 1.33e-62 199
MS.gene056355.t1 MTR_5g014520 42.593 324 144 9 17 308 20 333 4.81e-62 200
MS.gene056355.t1 MTR_5g014560 41.853 313 141 13 17 308 15 307 2.56e-54 179
MS.gene056355.t1 MTR_5g014600 35.673 342 156 10 13 304 7 334 2.74e-44 154
MS.gene056355.t1 MTR_4g009540 41.951 205 97 7 122 305 128 331 1.91e-42 149
MS.gene056355.t1 MTR_2g010450 43.548 186 91 3 134 306 168 352 6.99e-40 143
MS.gene056355.t1 MTR_4g097920 38.942 208 110 4 107 303 108 309 9.68e-40 141
MS.gene056355.t1 MTR_4g097920 38.942 208 110 4 107 303 123 324 1.11e-39 142
MS.gene056355.t1 MTR_4g009110 38.136 236 119 8 92 305 120 350 9.31e-39 140
MS.gene056355.t1 MTR_4g097940 38.462 208 111 4 107 303 123 324 9.33e-39 139
MS.gene056355.t1 MTR_4g097950 46.486 185 83 4 138 306 67 251 1.49e-38 137
MS.gene056355.t1 MTR_8g009020 38.660 194 103 2 128 305 130 323 1.83e-38 139
MS.gene056355.t1 MTR_4g092700 39.423 208 103 3 115 306 132 332 4.87e-38 137
MS.gene056355.t1 MTR_2g104550 39.216 204 111 4 117 308 113 315 1.92e-37 135
MS.gene056355.t1 MTR_2g104490 37.109 256 135 8 59 303 66 306 6.75e-37 134
MS.gene056355.t1 MTR_2g104650 40.084 237 118 8 80 302 40 266 1.57e-36 132
MS.gene056355.t1 MTR_5g014640 36.232 207 105 6 116 304 126 323 4.73e-36 132
MS.gene056355.t1 MTR_4g009540 41.304 184 86 7 122 284 128 310 5.61e-34 126
MS.gene056355.t1 MTR_7g080780 37.321 209 122 4 100 305 127 329 1.11e-32 123
MS.gene056355.t1 MTR_7g080780 37.321 209 122 4 100 305 114 316 1.13e-32 123
MS.gene056355.t1 MTR_2g104560 36.316 190 104 4 130 306 41 226 4.71e-32 119
MS.gene056355.t1 MTR_8g009010 36.406 217 103 4 92 300 78 267 6.84e-32 121
MS.gene056355.t1 MTR_8g069740 37.445 227 118 8 80 283 40 265 7.28e-32 120
MS.gene056355.t1 MTR_2g104490 36.709 237 124 8 59 284 66 287 5.68e-30 115
MS.gene056355.t1 MTR_2g104530 37.427 171 96 4 142 304 106 273 4.45e-29 112
MS.gene056355.t1 MTR_2g104590 36.957 184 83 6 130 301 58 220 1.15e-27 107
MS.gene056355.t1 MTR_2g104500 34.921 189 112 4 127 305 85 272 7.10e-27 106
MS.gene056355.t1 MTR_4g009510 37.423 163 96 5 143 303 45 203 7.31e-27 105
MS.gene056355.t1 MTR_7g451650 39.041 146 76 3 164 303 57 195 7.19e-24 96.7
MS.gene056355.t1 MTR_4g009420 40.714 140 74 3 164 303 65 195 3.32e-23 95.1
MS.gene056355.t1 MTR_7g050350 37.857 140 74 3 170 303 1 133 3.89e-22 90.5
MS.gene056355.t1 MTR_7g451650 37.857 140 74 3 170 303 1 133 3.89e-22 90.5
MS.gene056355.t1 MTR_5g014550 44.094 127 52 3 171 283 54 175 1.67e-19 85.1
MS.gene056355.t1 MTR_7g451650 36.641 131 70 3 164 288 57 180 1.29e-15 74.3
MS.gene056355.t1 MTR_4g009420 38.400 125 68 3 164 288 65 180 8.62e-15 72.4
MS.gene056355.t1 MTR_8g067280 29.240 171 81 4 133 268 444 609 1.76e-14 74.3
MS.gene056355.t1 MTR_8g024790 27.426 237 141 8 56 261 187 423 2.34e-14 73.6
MS.gene056355.t1 MTR_1g072320 31.034 145 77 3 141 265 470 611 3.02e-14 73.6
MS.gene056355.t1 MTR_5g030430 29.341 167 94 3 126 268 474 640 5.46e-14 72.8
MS.gene056355.t1 MTR_7g117670 31.169 154 101 2 137 285 394 547 1.12e-13 71.6
MS.gene056355.t1 MTR_8g024770 28.477 151 78 4 115 241 248 392 1.68e-13 70.9
MS.gene056355.t1 MTR_7g096350 36.082 97 52 1 108 194 312 408 2.26e-12 67.8
MS.gene056355.t1 MTR_8g027495 45.333 75 41 0 141 215 458 532 3.80e-12 67.0
MS.gene056355.t1 MTR_3g498695 26.984 189 117 3 127 295 284 471 7.16e-12 66.2
MS.gene056355.t1 MTR_8g064700 27.778 180 98 6 126 281 227 398 8.68e-12 65.9
MS.gene056355.t1 MTR_3g030700 32.692 156 89 4 143 283 198 352 3.88e-11 63.5
MS.gene056355.t1 MTR_3g498695 26.984 189 116 4 127 295 284 470 4.78e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056355.t1 AT2G22750 34.582 347 163 14 8 307 4 333 1.12e-37 137
MS.gene056355.t1 AT2G22750 34.118 340 161 13 15 307 6 329 2.43e-37 135
MS.gene056355.t1 AT4G37850 41.176 204 98 6 121 306 128 327 1.01e-36 134
MS.gene056355.t1 AT4G37850 41.176 204 98 6 121 306 128 327 1.01e-36 134
MS.gene056355.t1 AT2G22760 38.298 188 103 3 130 304 108 295 1.28e-36 133
MS.gene056355.t1 AT2G22750 34.582 347 162 15 8 307 4 332 1.65e-36 134
MS.gene056355.t1 AT2G22750 38.174 241 112 7 99 307 70 305 1.20e-34 128
MS.gene056355.t1 AT2G22750 38.174 241 111 8 99 307 70 304 2.23e-33 124
MS.gene056355.t1 AT2G22770 37.566 189 92 2 140 303 131 318 1.51e-31 120
MS.gene056355.t1 AT2G22760 38.323 167 91 2 130 284 108 274 1.05e-30 117
MS.gene056355.t1 AT5G46760 30.000 200 106 5 123 288 393 592 6.02e-19 87.4
MS.gene056355.t1 AT4G17880 33.918 171 73 6 126 266 397 557 1.93e-16 80.1
MS.gene056355.t1 AT1G32640 32.692 156 83 3 126 263 433 584 1.63e-15 77.4
MS.gene056355.t1 AT2G46510 37.607 117 54 4 133 239 377 484 6.47e-14 72.4
MS.gene056355.t1 AT1G01260 49.254 67 31 1 131 194 420 486 4.00e-13 70.1
MS.gene056355.t1 AT1G01260 49.254 67 31 1 131 194 420 486 4.00e-13 70.1
MS.gene056355.t1 AT1G01260 49.254 67 31 1 131 194 420 486 4.00e-13 70.1
MS.gene056355.t1 AT4G16430 46.575 73 35 1 126 194 301 373 6.14e-13 69.3
MS.gene056355.t1 AT4G09820 24.915 293 170 9 20 307 269 516 1.47e-12 68.2
MS.gene056355.t1 AT5G46830 54.839 62 24 1 137 194 335 396 3.44e-12 67.4
MS.gene056355.t1 AT5G41315 27.869 183 95 5 132 280 437 616 1.67e-11 65.5
MS.gene056355.t1 AT5G41315 27.869 183 95 5 132 280 432 611 1.68e-11 65.5
MS.gene056355.t1 AT5G41315 27.869 183 95 5 132 280 440 619 1.80e-11 65.1
MS.gene056355.t1 AT1G63650 28.378 148 79 4 141 261 405 552 1.94e-11 65.1
MS.gene056355.t1 AT1G63650 28.378 148 79 4 141 261 405 552 1.94e-11 65.1
MS.gene056355.t1 AT1G63650 28.378 148 79 4 141 261 405 552 1.94e-11 65.1

Find 46 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTTCCTAACCTTAAAA+AGG 0.267726 4.1:+34438051 MS.gene056355:CDS
CAGCAGCTGGTTATCTGATT+TGG 0.268145 4.1:+34437243 MS.gene056355:CDS
GGGGCTTCTGGTTAAAATTA+TGG 0.308732 4.1:+34439297 MS.gene056355:CDS
CAAGGAGCACAAGGGGCTTC+TGG 0.325264 4.1:+34439285 MS.gene056355:CDS
TCAAGAACAAAACAAACAAT+TGG 0.334442 4.1:+34437727 MS.gene056355:CDS
CAAGTGGAAGCAAGAGTGTT+AGG 0.334987 4.1:+34439235 MS.gene056355:CDS
ATCAGTGTCTCCAACCGAAT+TGG 0.364748 4.1:+34437862 MS.gene056355:CDS
CTGCAAGAGCAATGAAGCTC+TGG 0.379324 4.1:-34438027 None:intergenic
AATAATAGTGTCTTACCCTT+TGG 0.385740 4.1:+34439352 MS.gene056355:CDS
CAAGAACAAAACAAACAATT+GGG 0.390107 4.1:+34437728 MS.gene056355:CDS
CATCTGCATCAAGAAAATTA+AGG 0.394973 4.1:-34437667 None:intergenic
TTGATGATACTTTCATTGCT+TGG 0.395476 4.1:-34437219 None:intergenic
ATCGCTGCACATGCATGGTT+TGG 0.402273 4.1:-34439155 None:intergenic
ACTCTTGCTTCCACTTGAAA+TGG 0.409697 4.1:-34439229 None:intergenic
AGAAACCTCAAAAGGGTCAT+TGG 0.411401 4.1:+34437904 MS.gene056355:CDS
GCCCCTTGTGCTCCTTGCAT+TGG 0.417499 4.1:-34439279 None:intergenic
CACTCAACAGAAACCTCAAA+AGG 0.444007 4.1:+34437896 MS.gene056355:CDS
TAGGAAAACAAGTGTTGATT+CGG 0.449123 4.1:+34439254 MS.gene056355:CDS
TGACTTGCTGAGATCATTTG+TGG 0.473303 4.1:-34437759 None:intergenic
GCTGAGATCATTTGTGGAAG+TGG 0.497595 4.1:-34437753 None:intergenic
AGCAAAAGGCCTCGTGCTCA+TGG 0.502148 4.1:+34437959 MS.gene056355:CDS
GGTAGCTACCTGAGCAATGA+TGG 0.511294 4.1:-34439395 None:intergenic
GATAACATTGAATGTGTTGT+TGG 0.511734 4.1:+34439199 MS.gene056355:CDS
AAGGCCTCGTGCTCATGGTA+AGG 0.535577 4.1:+34437964 MS.gene056355:CDS
TTCTTGATGCAGATGTGGGA+GGG 0.544131 4.1:+34437675 MS.gene056355:CDS
CGACATTACCATCATTGCTC+AGG 0.544333 4.1:+34439387 MS.gene056355:CDS
GTTGATTCGGTTCCAATGCA+AGG 0.545125 4.1:+34439267 MS.gene056355:CDS
TCTTCATCGCTGCACATGCA+TGG 0.556450 4.1:-34439160 None:intergenic
GTTCCAATGCAAGGAGCACA+AGG 0.563766 4.1:+34439276 MS.gene056355:CDS
TGCAAGAGCAATGAAGCTCT+GGG 0.565077 4.1:-34438026 None:intergenic
AAGTATCATCAATCAGCAGC+TGG 0.573007 4.1:+34437230 MS.gene056355:CDS
TTTCTTGATGCAGATGTGGG+AGG 0.583981 4.1:+34437674 MS.gene056355:CDS
ACTCAACAGAAACCTCAAAA+GGG 0.587077 4.1:+34437897 MS.gene056355:CDS
GTCGAGTGTAGAATCTCCAA+AGG 0.590011 4.1:-34439368 None:intergenic
TCCAATGCAAGGAGCACAAG+GGG 0.595381 4.1:+34439278 MS.gene056355:CDS
AGGGTACAACTTGAGCATAA+AGG 0.600355 4.1:+34439590 MS.gene056355:CDS
TGGTACTTCACCATTTCAAG+TGG 0.606706 4.1:+34439219 MS.gene056355:CDS
TCATGGTAAGGATCACATCA+TGG 0.611981 4.1:+34437976 MS.gene056355:CDS
CTGCACATGCATGGTTTGGT+CGG 0.612901 4.1:-34439151 None:intergenic
TCGAGTGTAGAATCTCCAAA+GGG 0.615421 4.1:-34439367 None:intergenic
CGAATACAATATATTTGCTG+AGG 0.624364 4.1:+34437637 MS.gene056355:CDS
TGAAGATTCGTCATCATGTG+AGG 0.642706 4.1:+34439177 MS.gene056355:CDS
TTCCAATGCAAGGAGCACAA+GGG 0.644827 4.1:+34439277 MS.gene056355:CDS
AAGAACAAAACAAACAATTG+GGG 0.650416 4.1:+34437729 MS.gene056355:CDS
ACTTGTGAAGAACGTACGCA+TGG 0.661373 4.1:+34439614 MS.gene056355:CDS
TGATCCTTACCATGAGCACG+AGG 0.705697 4.1:-34437968 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGATGTATAATAAATA+GGG - chr4.1:34438709-34438728 None:intergenic 10.0%
!!! ATCATATATTATTTTATAGT+TGG + chr4.1:34439563-34439582 MS.gene056355:intron 10.0%
!!! GTTTATATTTTGAAATATTT+AGG + chr4.1:34438323-34438342 MS.gene056355:intron 10.0%
!!! TCATATATTATTTTATAGTT+GGG + chr4.1:34439564-34439583 MS.gene056355:intron 10.0%
!!! TTTATTATTTTTCTTTTAGA+TGG + chr4.1:34439048-34439067 MS.gene056355:intron 10.0%
!! AAAAGATGTATAATAAATAG+GGG - chr4.1:34438708-34438727 None:intergenic 15.0%
!! AAAGAAATAATTAATGTAGT+TGG - chr4.1:34438676-34438695 None:intergenic 15.0%
!! ATGATTAATATTAGATACAA+GGG - chr4.1:34439548-34439567 None:intergenic 15.0%
!! CATTAAAAAAAAATTGTGAT+AGG - chr4.1:34439419-34439438 None:intergenic 15.0%
!! GAAAAAGATGTATAATAAAT+AGG - chr4.1:34438710-34438729 None:intergenic 15.0%
!! TATGATTAATATTAGATACA+AGG - chr4.1:34439549-34439568 None:intergenic 15.0%
!!! AGACAAATCAATTTTATTTA+AGG + chr4.1:34438990-34439009 MS.gene056355:intron 15.0%
!!! CTTACATTTAACTTAATTAA+TGG - chr4.1:34437347-34437366 None:intergenic 15.0%
!! AAAAAAAAATCTTGAAGACT+TGG - chr4.1:34437509-34437528 None:intergenic 20.0%
!! CACAAAAATTGTCAAAAAAA+TGG - chr4.1:34438436-34438455 None:intergenic 20.0%
!! CCTTAAATAAGAATAAACAT+AGG + chr4.1:34437442-34437461 MS.gene056355:intron 20.0%
!! TACATATGACATATATGAAT+TGG - chr4.1:34438268-34438287 None:intergenic 20.0%
!!! CCTATGTTTATTCTTATTTA+AGG - chr4.1:34437445-34437464 None:intergenic 20.0%
!!! GACAAAAAATTTTCTCTAAA+GGG - chr4.1:34438626-34438645 None:intergenic 20.0%
!!! GTTTATTCTTATTTAAGGTT+AGG - chr4.1:34437440-34437459 None:intergenic 20.0%
!!! TCATTCTCTTTTGAAATATT+TGG - chr4.1:34437836-34437855 None:intergenic 20.0%
!!! TGAACTTTTATTTTTTTTGC+AGG + chr4.1:34437607-34437626 MS.gene056355:intron 20.0%
!!! TTACATAACATACCTTTTTA+AGG - chr4.1:34438066-34438085 None:intergenic 20.0%
!!! TTTATTTTTTTTGCAGGAAA+TGG + chr4.1:34437613-34437632 MS.gene056355:intron 20.0%
! AAAAAAATGCATGAAAACCA+AGG - chr4.1:34438509-34438528 None:intergenic 25.0%
! AAGAACAAAACAAACAATTG+GGG + chr4.1:34437729-34437748 MS.gene056355:CDS 25.0%
! ACCAAAAAAAAAAGCTAATG+GGG - chr4.1:34438099-34438118 None:intergenic 25.0%
! ATATTATTTGTCTCAACGTT+TGG + chr4.1:34437555-34437574 MS.gene056355:intron 25.0%
! CAAGAACAAAACAAACAATT+GGG + chr4.1:34437728-34437747 MS.gene056355:CDS 25.0%
! CACCAAAAAAAAAAGCTAAT+GGG - chr4.1:34438100-34438119 None:intergenic 25.0%
! CTTTGACATATAATTATGAG+CGG + chr4.1:34438906-34438925 MS.gene056355:intron 25.0%
! GAGGTAGTATATTGTTTATA+TGG - chr4.1:34438586-34438605 None:intergenic 25.0%
! GTAGTATATTGTTTATATGG+AGG - chr4.1:34438583-34438602 None:intergenic 25.0%
! TAATCAACCATCTTTGAATT+TGG - chr4.1:34437308-34437327 None:intergenic 25.0%
! TCAAGAACAAAACAAACAAT+TGG + chr4.1:34437727-34437746 MS.gene056355:CDS 25.0%
! TTTCTTCAGACAATAATTGA+TGG + chr4.1:34437527-34437546 MS.gene056355:intron 25.0%
!! AACATGTTATTTATTGCTGA+GGG - chr4.1:34438735-34438754 None:intergenic 25.0%
!! ATTTCCGTTATGTTTTCAAA+AGG + chr4.1:34438846-34438865 MS.gene056355:intron 25.0%
!! GATTTGGTAAGAAAATCAAA+AGG + chr4.1:34437259-34437278 MS.gene056355:intron 25.0%
!! GGACAAAAAATTTTCTCTAA+AGG - chr4.1:34438627-34438646 None:intergenic 25.0%
!! TCTAAAGGGTCTTATAATTA+GGG - chr4.1:34438612-34438631 None:intergenic 25.0%
!! TTCTTATTTAAGGTTAGGTT+AGG - chr4.1:34437435-34437454 None:intergenic 25.0%
!! TTTCCGTTATGTTTTCAAAA+GGG + chr4.1:34438847-34438866 MS.gene056355:intron 25.0%
!!! ATGCTATTTTACTCAATCAT+AGG + chr4.1:34437388-34437407 MS.gene056355:intron 25.0%
!!! ATTATTTTATAGTTGGGTGA+AGG + chr4.1:34439570-34439589 MS.gene056355:intron 25.0%
!!! TAACATACCTTTTTAAGGTT+AGG - chr4.1:34438061-34438080 None:intergenic 25.0%
!!! TTATTTTATAGTTGGGTGAA+GGG + chr4.1:34439571-34439590 MS.gene056355:intron 25.0%
AATAATAGTGTCTTACCCTT+TGG + chr4.1:34439352-34439371 MS.gene056355:CDS 30.0%
AATGTAGTTGGAAATAGCAA+AGG - chr4.1:34438664-34438683 None:intergenic 30.0%
ACCTTCTCAAATAGATTTGT+AGG + chr4.1:34437483-34437502 MS.gene056355:intron 30.0%
ACTTTATCTCTTTATTGCCT+TGG + chr4.1:34438489-34438508 MS.gene056355:intron 30.0%
AGATACAAGGGTAAATTTCA+TGG - chr4.1:34439536-34439555 None:intergenic 30.0%
CAAAAACCTCAAAAAGCAAA+AGG + chr4.1:34437945-34437964 MS.gene056355:CDS 30.0%
CATAATATATGCAAGGTTTG+GGG - chr4.1:34438138-34438157 None:intergenic 30.0%
CATCTGCATCAAGAAAATTA+AGG - chr4.1:34437670-34437689 None:intergenic 30.0%
CCACCAAAAAAAAAAGCTAA+TGG - chr4.1:34438101-34438120 None:intergenic 30.0%
GACAATGCATAATATATGCA+AGG - chr4.1:34438145-34438164 None:intergenic 30.0%
GATAACATTGAATGTGTTGT+TGG + chr4.1:34439199-34439218 MS.gene056355:CDS 30.0%
GCATAATATATGCAAGGTTT+GGG - chr4.1:34438139-34438158 None:intergenic 30.0%
TCTTGTTCCTAACCTTAAAA+AGG + chr4.1:34438051-34438070 MS.gene056355:CDS 30.0%
TGCATAATATATGCAAGGTT+TGG - chr4.1:34438140-34438159 None:intergenic 30.0%
! AATTTTCTTGATGCAGATGT+GGG + chr4.1:34437671-34437690 MS.gene056355:CDS 30.0%
! AGCAAAGGAGTCTTATAAAA+AGG - chr4.1:34438649-34438668 None:intergenic 30.0%
! ATTTAAGGTTAGGTTAGGTT+AGG - chr4.1:34437430-34437449 None:intergenic 30.0%
! CAACATGTTATTTATTGCTG+AGG - chr4.1:34438736-34438755 None:intergenic 30.0%
! CGAATACAATATATTTGCTG+AGG + chr4.1:34437637-34437656 MS.gene056355:CDS 30.0%
! CTCTAAAGGGTCTTATAATT+AGG - chr4.1:34438613-34438632 None:intergenic 30.0%
! GCAAAGGAGTCTTATAAAAA+GGG - chr4.1:34438648-34438667 None:intergenic 30.0%
! GTGATTCATGTTTTCCAATT+CGG - chr4.1:34437879-34437898 None:intergenic 30.0%
! TAATTTTCTTGATGCAGATG+TGG + chr4.1:34437670-34437689 MS.gene056355:CDS 30.0%
! TTGATGATACTTTCATTGCT+TGG - chr4.1:34437222-34437241 None:intergenic 30.0%
!! TAGGAAAACAAGTGTTGATT+CGG + chr4.1:34439254-34439273 MS.gene056355:CDS 30.0%
!!! ACCCCATTAGCTTTTTTTTT+TGG + chr4.1:34438095-34438114 MS.gene056355:intron 30.0%
!!! CCATTAGCTTTTTTTTTTGG+TGG + chr4.1:34438098-34438117 MS.gene056355:intron 30.0%
!!! TTTTTTCCACAACTTCCAAA+CGG - chr4.1:34439126-34439145 None:intergenic 30.0%
!!! TTTTTTTTTTGGTGGTGTCA+GGG + chr4.1:34438106-34438125 MS.gene056355:intron 30.0%
AAACCAACTCTCTCTCTTAA+AGG + chr4.1:34438763-34438782 MS.gene056355:intron 35.0%
ACTCAACAGAAACCTCAAAA+GGG + chr4.1:34437897-34437916 MS.gene056355:CDS 35.0%
CATCTTTGAATTTGGAAAGC+AGG - chr4.1:34437300-34437319 None:intergenic 35.0%
CTGCTTTCCAAATTCAAAGA+TGG + chr4.1:34437298-34437317 MS.gene056355:intron 35.0%
GAAAGAGCTTAAAAACCGTT+TGG + chr4.1:34439108-34439127 MS.gene056355:CDS 35.0%
GCCTACAAATCTATTTGAGA+AGG - chr4.1:34437487-34437506 None:intergenic 35.0%
TAAAAACCGTTTGGAAGTTG+TGG + chr4.1:34439117-34439136 MS.gene056355:CDS 35.0%
TACTCAATCATAGGTCTCAA+GGG + chr4.1:34437397-34437416 MS.gene056355:intron 35.0%
TCAAAAGGGCCCTATAATTA+GGG + chr4.1:34438861-34438880 MS.gene056355:intron 35.0%
TTACTCAATCATAGGTCTCA+AGG + chr4.1:34437396-34437415 MS.gene056355:intron 35.0%
TTCAAAAGGGCCCTATAATT+AGG + chr4.1:34438860-34438879 MS.gene056355:intron 35.0%
TTGAATTTGGAAAGCAGGAA+AGG - chr4.1:34437295-34437314 None:intergenic 35.0%
! AAGGGTCTTATAATTAGGGA+CGG - chr4.1:34438608-34438627 None:intergenic 35.0%
! ATTTTCCAATGACCCTTTTG+AGG - chr4.1:34437912-34437931 None:intergenic 35.0%
! TATCCTTTAAGAGAGAGAGT+TGG - chr4.1:34438769-34438788 None:intergenic 35.0%
! TTCATGTTTTCCAATTCGGT+TGG - chr4.1:34437875-34437894 None:intergenic 35.0%
!!! CTTTTTTTTTTGGTGGTGTC+AGG + chr4.1:34438105-34438124 MS.gene056355:intron 35.0%
AAGTATCATCAATCAGCAGC+TGG + chr4.1:34437230-34437249 MS.gene056355:CDS 40.0%
ACTCTTGCTTCCACTTGAAA+TGG - chr4.1:34439232-34439251 None:intergenic 40.0%
AGAAACCTCAAAAGGGTCAT+TGG + chr4.1:34437904-34437923 MS.gene056355:CDS 40.0%
AGGGTACAACTTGAGCATAA+AGG + chr4.1:34439590-34439609 MS.gene056355:CDS 40.0%
CACTCAACAGAAACCTCAAA+AGG + chr4.1:34437896-34437915 MS.gene056355:CDS 40.0%
TCATGGTAAGGATCACATCA+TGG + chr4.1:34437976-34437995 MS.gene056355:CDS 40.0%
TCGAGTGTAGAATCTCCAAA+GGG - chr4.1:34439370-34439389 None:intergenic 40.0%
TGAAGATTCGTCATCATGTG+AGG + chr4.1:34439177-34439196 MS.gene056355:CDS 40.0%
TGGTACTTCACCATTTCAAG+TGG + chr4.1:34439219-34439238 MS.gene056355:CDS 40.0%
! CGAGCTTAACTCAGTTGATA+AGG - chr4.1:34438167-34438186 None:intergenic 40.0%
! GGGGCTTCTGGTTAAAATTA+TGG + chr4.1:34439297-34439316 MS.gene056355:CDS 40.0%
!! GGAGGAGTGTTATTTGAACT+TGG - chr4.1:34438565-34438584 None:intergenic 40.0%
!! GGGCCCTTTTGAAAACATAA+CGG - chr4.1:34438853-34438872 None:intergenic 40.0%
!! TGACTTGCTGAGATCATTTG+TGG - chr4.1:34437762-34437781 None:intergenic 40.0%
AAGGGCCCTATAATTAGGGA+CGG + chr4.1:34438865-34438884 MS.gene056355:intron 45.0%
ACTTGTGAAGAACGTACGCA+TGG + chr4.1:34439614-34439633 MS.gene056355:CDS 45.0%
ATCAACTGAGTTAAGCTCGC+AGG + chr4.1:34438168-34438187 MS.gene056355:intron 45.0%
ATCAGTGTCTCCAACCGAAT+TGG + chr4.1:34437862-34437881 MS.gene056355:CDS 45.0%
CAGCAGCTGGTTATCTGATT+TGG + chr4.1:34437243-34437262 MS.gene056355:CDS 45.0%
CGACATTACCATCATTGCTC+AGG + chr4.1:34439387-34439406 MS.gene056355:CDS 45.0%
GCTGAGATCATTTGTGGAAG+TGG - chr4.1:34437756-34437775 None:intergenic 45.0%
GTCGAGTGTAGAATCTCCAA+AGG - chr4.1:34439371-34439390 None:intergenic 45.0%
TGCAAGAGCAATGAAGCTCT+GGG - chr4.1:34438029-34438048 None:intergenic 45.0%
TGCCTCCGTCCCTAATTATA+GGG - chr4.1:34438873-34438892 None:intergenic 45.0%
TTCTTGATGCAGATGTGGGA+GGG + chr4.1:34437675-34437694 MS.gene056355:CDS 45.0%
TTTCTTGATGCAGATGTGGG+AGG + chr4.1:34437674-34437693 MS.gene056355:CDS 45.0%
! GGTCTTATAATTAGGGACGG+AGG - chr4.1:34438605-34438624 None:intergenic 45.0%
! TTCCAATGCAAGGAGCACAA+GGG + chr4.1:34439277-34439296 MS.gene056355:CDS 45.0%
!! CAAGTGGAAGCAAGAGTGTT+AGG + chr4.1:34439235-34439254 MS.gene056355:CDS 45.0%
!! GTTGATTCGGTTCCAATGCA+AGG + chr4.1:34439267-34439286 MS.gene056355:CDS 45.0%
!!! ACGAGGCCTTTTGCTTTTTG+AGG - chr4.1:34437954-34437973 None:intergenic 45.0%
!!! CATTTTTTTTTTTTAAATTA+TGG + chr4.1:34438520-34438539 MS.gene056355:intron 5.0%
!!! TTATTTTGTTTTTTTTATTT+TGG + chr4.1:34439500-34439519 MS.gene056355:intron 5.0%
!!! TTTATATTTTGAAATATTTA+GGG + chr4.1:34438324-34438343 MS.gene056355:intron 5.0%
ATCGCTGCACATGCATGGTT+TGG - chr4.1:34439158-34439177 None:intergenic 50.0%
CTGCAAGAGCAATGAAGCTC+TGG - chr4.1:34438030-34438049 None:intergenic 50.0%
CTGCCTCCGTCCCTAATTAT+AGG - chr4.1:34438874-34438893 None:intergenic 50.0%
GGTAGCTACCTGAGCAATGA+TGG - chr4.1:34439398-34439417 None:intergenic 50.0%
TCTTCATCGCTGCACATGCA+TGG - chr4.1:34439163-34439182 None:intergenic 50.0%
TGATCCTTACCATGAGCACG+AGG - chr4.1:34437971-34437990 None:intergenic 50.0%
! GTTCCAATGCAAGGAGCACA+AGG + chr4.1:34439276-34439295 MS.gene056355:CDS 50.0%
! TCCAATGCAAGGAGCACAAG+GGG + chr4.1:34439278-34439297 MS.gene056355:CDS 50.0%
!! CTGCACATGCATGGTTTGGT+CGG - chr4.1:34439154-34439173 None:intergenic 50.0%
AAGGCCTCGTGCTCATGGTA+AGG + chr4.1:34437964-34437983 MS.gene056355:CDS 55.0%
AGCAAAAGGCCTCGTGCTCA+TGG + chr4.1:34437959-34437978 MS.gene056355:CDS 55.0%
GGCCCTATAATTAGGGACGG+AGG + chr4.1:34438868-34438887 MS.gene056355:intron 55.0%
GCCCCTTGTGCTCCTTGCAT+TGG - chr4.1:34439282-34439301 None:intergenic 60.0%
! CAAGGAGCACAAGGGGCTTC+TGG + chr4.1:34439285-34439304 MS.gene056355:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 34437202 34439662 34437202 ID=MS.gene056355
chr4.1 mRNA 34437202 34439662 34437202 ID=MS.gene056355.t1;Parent=MS.gene056355
chr4.1 exon 34437202 34437264 34437202 ID=MS.gene056355.t1.exon1;Parent=MS.gene056355.t1
chr4.1 CDS 34437202 34437264 34437202 ID=cds.MS.gene056355.t1;Parent=MS.gene056355.t1
chr4.1 exon 34437629 34438072 34437629 ID=MS.gene056355.t1.exon2;Parent=MS.gene056355.t1
chr4.1 CDS 34437629 34438072 34437629 ID=cds.MS.gene056355.t1;Parent=MS.gene056355.t1
chr4.1 exon 34439067 34439408 34439067 ID=MS.gene056355.t1.exon3;Parent=MS.gene056355.t1
chr4.1 CDS 34439067 34439408 34439067 ID=cds.MS.gene056355.t1;Parent=MS.gene056355.t1
chr4.1 exon 34439582 34439662 34439582 ID=MS.gene056355.t1.exon4;Parent=MS.gene056355.t1
chr4.1 CDS 34439582 34439662 34439582 ID=cds.MS.gene056355.t1;Parent=MS.gene056355.t1
Gene Sequence

>MS.gene056355

ATGGAGGAAATCAACAACCAAGCAATGAAAGTATCATCAATCAGCAGCTGGTTATCTGATTTGGTAAGAAAATCAAAAGGCAATTATTCACCTTTCCTGCTTTCCAAATTCAAAGATGGTTGATTATTAATTAATTCTTTTGCCATTAATTAAGTTAAATGTAAGCTAAGTGAAGCTTATAATCTTATGCTATTTTACTCAATCATAGGTCTCAAGGGAAATAAGCCTAACCTAACCTAACCTTAAATAAGAATAAACATAGGTCTCAAATATAGAGTTATACCTTCTCAAATAGATTTGTAGGCCAAGTCTTCAAGATTTTTTTTTTCTTCAGACAATAATTGATGGACTTTATATTATTTGTCTCAACGTTTGGAATAATATAGTCATTAATTAATTAACACTTGAACTTTTATTTTTTTTGCAGGAAATGGACGAATACAATATATTTGCTGAGGAATGCAACCTTAATTTTCTTGATGCAGATGTGGGAGGGTTTCTTTCAGATGACATATCTACTGTATTTCAAGAACAAAACAAACAATTGGGGTCCACTTCCACAAATGATCTCAGCAAGTCATTCATTCATGAAACAATTGATAATAATGACAAAATCAATGTATCTCTTTCACCAAATATTTCAAAAGAGAATGAAACAATATCAGTGTCTCCAACCGAATTGGAAAACATGAATCACTCAACAGAAACCTCAAAAGGGTCATTGGAAAATGAAAAGTTTGAAACAAAAACCTCAAAAAGCAAAAGGCCTCGTGCTCATGGTAAGGATCACATCATGGCTGAGAGAAATCGTAGAGAGAAACTCACCCAGAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTTAAAAAGGTATGTTATGTAATTTTCACTTACCCCATTAGCTTTTTTTTTTGGTGGTGTCAGGGTTTGAACCCCAAACCTTGCATATATTATGCATTGTCCTTATCAACTGAGTTAAGCTCGCAGGACACTTACTCTATTAGCTAACTCTTAGAATCATCAATTATCAATCAATTACTTCATCGATAAATACATGTTGCACCAATTCATATATGTCATATGTAAGATTTTGATCACTAATATCTTTAAATATTTAAAAGTTTATATTTTGAAATATTTAGGGAGACAAATCGAATATCTTATATATTAACATTTCTCTTTACATATGATCAAAGTATGTTATATGAATGAGAAATGATATTTGAACATCCATTTTTTTGACAATTTTTGTGATAATTTTCTCTCTCATACTCACATTATTTTTTACTTTATCTCTTTATTGCCTTGGTTTTCATGCATTTTTTTTTTTTAAATTATGGTTGTAATATAAGTTGTCAACCAAGTTCAAATAACACTCCTCCATATAAACAATATACTACCTCCGTCCCTAATTATAAGACCCTTTAGAGAAAATTTTTTGTCCCTTTTTATAAGACTCCTTTGCTATTTCCAACTACATTAATTATTTCTTTACATACATGCCCCTATTTATTATACATCTTTTTCTTCCCTCAGCAATAAATAACATGTTGAAAACATAAACCAACTCTCTCTCTTAAAGGATAAAATTGTAAAAACATTAGTAATTACAAACATATTTAATACAAATATCTACTATCTTAATTTCCGTTATGTTTTCAAAAGGGCCCTATAATTAGGGACGGAGGCAGTACATAGTAAAACTTTGACATATAATTATGAGCGGAGAGTACAAATATAAGAAAATTAATTACATATTTAATACATTCTTATCTTTCTTTAAAATAAGACAAATCAATTTTATTTAAGGACAAAACTATCATTTAACATTATTATTTCTAACTCTTTATTATTTTTCTTTTAGATGGACAAGTTATCTGTACTAATTGACACTATCAAATACTTGAAAGAGCTTAAAAACCGTTTGGAAGTTGTGGAAAAAAAGTCACCGACCAAACCATGCATGTGCAGCGATGAAGATTCGTCATCATGTGAGGATAACATTGAATGTGTTGTTGGTACTTCACCATTTCAAGTGGAAGCAAGAGTGTTAGGAAAACAAGTGTTGATTCGGTTCCAATGCAAGGAGCACAAGGGGCTTCTGGTTAAAATTATGGTCGAAATTCAAAAATTTCAACTATTTGTTGTCAATAATAGTGTCTTACCCTTTGGAGATTCTACACTCGACATTACCATCATTGCTCAGGTAGCTACCTATCACAATTTTTTTTTAATGTTATTATCTTATAATAAGTAGTTTATTCTAAGTTGTTTTCTACAATAATTGCGTGATATATTTATTTTGTTTTTTTTATTTTGGTCTTTTTTCTCCATGAAATTTACCCTTGTATCTAATATTAATCATATATTATTTTATAGTTGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTGAAGAACGTACGCATGGCATTATTGAAGTTTACGTCATCATAA
Protein sequence

>MS.gene056355.t1

MEEINNQAMKVSSISSWLSDLEMDEYNIFAEECNLNFLDADVGGFLSDDISTVFQEQNKQLGSTSTNDLSKSFIHETIDNNDKINVSLSPNISKENETISVSPTELENMNHSTETSKGSLENEKFETKTSKSKRPRAHGKDHIMAERNRREKLTQSFIALAALVPNLKKMDKLSVLIDTIKYLKELKNRLEVVEKKSPTKPCMCSDEDSSSCEDNIECVVGTSPFQVEARVLGKQVLIRFQCKEHKGLLVKIMVEIQKFQLFVVNNSVLPFGDSTLDITIIAQLGEGYNLSIKELVKNVRMALLKFTSS