AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056372

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056372.t1 MTR_0856s0010 60.625 160 61 1 1 158 1 160 5.33e-58 194
MS.gene056372.t1 MTR_6g051425 31.618 272 174 5 35 296 20 289 1.43e-37 144
MS.gene056372.t1 MTR_7g115320 67.470 83 24 1 119 201 6 85 5.72e-28 108
MS.gene056372.t1 MTR_4g035820 29.779 272 153 11 25 273 68 324 2.95e-21 99.4
MS.gene056372.t1 MTR_0558s0020 26.255 259 164 6 36 269 40 296 4.52e-21 94.7
MS.gene056372.t1 MTR_1g008290 33.333 129 83 1 97 225 19 144 9.01e-20 87.0
MS.gene056372.t1 MTR_8g446020 28.729 181 123 3 93 269 78 256 3.51e-18 85.5
MS.gene056372.t1 MTR_1g070355 27.966 236 154 9 38 263 80 309 1.41e-17 87.4
MS.gene056372.t1 MTR_7g446060 43.662 71 40 0 126 196 18 88 1.46e-16 77.4
MS.gene056372.t1 MTR_7g446510 30.168 179 123 2 105 281 155 333 1.81e-16 81.6
MS.gene056372.t1 MTR_7g446480 30.726 179 122 2 105 281 140 318 1.81e-16 81.6
MS.gene056372.t1 MTR_6g022070 29.605 152 91 2 323 468 1 142 1.67e-15 79.0
MS.gene056372.t1 MTR_5g076070 25.862 232 158 6 35 257 56 282 6.19e-14 73.6
MS.gene056372.t1 MTR_1g083640 24.348 230 147 6 36 240 55 282 4.37e-13 70.9
MS.gene056372.t1 MTR_5g023310 28.846 156 44 2 164 319 16 104 7.26e-13 65.9
MS.gene056372.t1 MTR_6g090505 25.843 178 130 2 93 268 53 230 9.30e-13 70.9
MS.gene056372.t1 MTR_1g013060 26.887 212 137 6 31 229 23 229 1.83e-12 68.2
MS.gene056372.t1 MTR_1g019850 31.788 151 92 5 14 160 53 196 6.92e-12 66.6
MS.gene056372.t1 MTR_7g446520 27.815 151 108 1 122 271 165 315 7.12e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056372.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene056372.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene056372.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene056372.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene056372.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene056372.t1 AT4G38180 34.750 659 382 15 17 637 47 695 6.19e-121 381
MS.gene056372.t1 AT1G52520 33.441 619 392 11 32 643 85 690 1.49e-113 359
MS.gene056372.t1 AT1G76320 30.945 656 414 11 36 685 1 623 5.78e-106 338
MS.gene056372.t1 AT1G76320 30.687 655 417 10 36 685 1 623 2.41e-105 338
MS.gene056372.t1 AT1G76320 30.687 655 417 10 36 685 1 623 3.69e-105 338
MS.gene056372.t1 AT1G76320 30.687 655 417 10 36 685 1 623 3.69e-105 338
MS.gene056372.t1 AT3G22170 31.994 622 370 8 26 628 64 651 4.94e-105 340
MS.gene056372.t1 AT3G22170 31.994 622 370 8 26 628 64 651 4.94e-105 340
MS.gene056372.t1 AT2G27110 32.097 620 376 13 18 628 32 615 9.58e-97 318
MS.gene056372.t1 AT2G27110 32.097 620 376 13 18 628 32 615 9.58e-97 318
MS.gene056372.t1 AT1G80010 30.366 629 410 11 32 643 95 712 1.74e-93 307
MS.gene056372.t1 AT2G32250 30.887 654 390 10 24 670 26 624 5.23e-92 304
MS.gene056372.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.19e-91 303
MS.gene056372.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.19e-91 303
MS.gene056372.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.81e-91 303
MS.gene056372.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.81e-91 303
MS.gene056372.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.81e-91 303
MS.gene056372.t1 AT2G32250 30.721 638 384 9 36 670 1 583 6.10e-90 298
MS.gene056372.t1 AT2G32250 30.721 638 384 9 36 670 1 583 6.16e-90 298
MS.gene056372.t1 AT3G06250 32.566 565 328 13 29 589 187 702 6.79e-89 295
MS.gene056372.t1 AT3G06250 32.566 565 328 13 29 589 187 702 6.79e-89 295
MS.gene056372.t1 AT3G06250 32.566 565 328 13 29 589 187 702 6.79e-89 295
MS.gene056372.t1 AT2G27110 35.388 438 268 6 194 628 45 470 4.10e-86 286
MS.gene056372.t1 AT4G19990 30.757 621 371 11 15 617 4 583 7.29e-85 284
MS.gene056372.t1 AT4G19990 30.757 621 371 11 15 617 18 597 9.17e-85 285
MS.gene056372.t1 AT4G19990 30.757 621 371 11 15 617 4 583 9.93e-85 284
MS.gene056372.t1 AT4G19990 30.757 621 371 11 15 617 4 583 9.93e-85 284
MS.gene056372.t1 AT4G19990 30.757 621 371 11 15 617 4 583 1.74e-84 284
MS.gene056372.t1 AT4G19990 30.757 621 371 11 15 617 4 583 1.74e-84 284
MS.gene056372.t1 AT5G18960 30.998 571 328 12 31 589 210 726 1.78e-82 279
MS.gene056372.t1 AT5G18960 30.998 571 328 12 31 589 210 726 1.78e-82 279
MS.gene056372.t1 AT4G38170 36.082 388 237 5 206 591 21 399 5.35e-82 271
MS.gene056372.t1 AT4G19990 35.800 419 261 4 201 617 114 526 1.13e-78 266
MS.gene056372.t1 AT1G10240 27.740 584 368 16 32 588 48 604 1.31e-60 216
MS.gene056372.t1 AT1G10240 27.740 584 368 16 32 588 48 604 1.31e-60 216
MS.gene056372.t1 AT5G28530 25.473 581 388 13 32 588 55 614 6.88e-54 197
MS.gene056372.t1 AT5G28530 25.473 581 388 13 32 588 55 614 6.88e-54 197
MS.gene056372.t1 AT5G28530 25.473 581 392 13 32 588 55 618 2.79e-53 196
MS.gene056372.t1 AT5G28530 24.214 318 219 5 275 588 252 551 1.21e-25 112
MS.gene056372.t1 AT5G28530 24.214 318 223 5 275 588 252 555 5.35e-25 110

Find 110 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCTGCATATGAATATTT+TGG 0.129890 4.1:+34171373 MS.gene056372:CDS
CACGATCAAAAGAAACTTTA+AGG 0.142944 4.1:-34172254 None:intergenic
TAAAGAATATGCTTCTAAAA+AGG 0.156428 4.1:+34170790 MS.gene056372:CDS
TCCCTTCGAATAATTCTATT+TGG 0.180280 4.1:-34172642 None:intergenic
GTTACCTTCCACGATATTTA+AGG 0.186766 4.1:-34172172 None:intergenic
TCGCATGAGTATATTCTTCT+TGG 0.219492 4.1:-34172098 None:intergenic
CAAATCTAGCTTGCTATGTT+TGG 0.233030 4.1:+34172578 MS.gene056372:CDS
CGCCCGCTTCATCATTGATT+TGG 0.236954 4.1:-34171102 None:intergenic
AGCATACATGCTTTCTTTGA+TGG 0.264138 4.1:+34171925 MS.gene056372:CDS
ATATACTCATGCGAAGTTTA+AGG 0.285045 4.1:+34172107 MS.gene056372:CDS
AAAAGGCATATCATATTTAT+TGG 0.296866 4.1:-34171428 None:intergenic
TGAAGACACTTCCGACTAAA+AGG 0.299870 4.1:+34170924 MS.gene056372:CDS
TTCATGTACGAAACGTGTTT+TGG 0.310952 4.1:+34171335 MS.gene056372:CDS
TGCTCATTGTTTGAGTTTAG+AGG 0.314378 4.1:+34172300 MS.gene056372:CDS
ACATAATTGGCACCCATCTA+TGG 0.318132 4.1:-34171855 None:intergenic
CATGGTCAATCAACTTTACT+TGG 0.319017 4.1:+34171475 MS.gene056372:CDS
CCTGAAAAGCTTAGTAGATA+TGG 0.320188 4.1:+34171688 MS.gene056372:CDS
GTGTTGGAGGAGATGTTAAT+TGG 0.326688 4.1:+34172210 MS.gene056372:CDS
TTTCTTCTATGATATAGATT+TGG 0.334701 4.1:+34171303 MS.gene056372:CDS
TGAATTCATGAAGTGTTTCA+TGG 0.341741 4.1:-34172557 None:intergenic
AACTTAGCAGTAATCTTTGC+AGG 0.345757 4.1:-34170956 None:intergenic
AAATCTAGCTTGCTATGTTT+GGG 0.356420 4.1:+34172579 MS.gene056372:CDS
TCACAAACATCAAAATTGTT+TGG 0.358937 4.1:-34170656 None:intergenic
AGTTGGGAAAAGATGAATTA+TGG 0.360559 4.1:+34170975 MS.gene056372:CDS
AACTTTACTTGGATGTGGTC+TGG 0.388630 4.1:+34171486 MS.gene056372:CDS
CTTGAATAACCTTGACTTAG+TGG 0.398152 4.1:-34171041 None:intergenic
GCAAGTTGCGAGCCTTCATC+AGG 0.400046 4.1:+34170728 MS.gene056372:CDS
TGTACAATGAGCGCCATAGA+TGG 0.404513 4.1:+34171842 MS.gene056372:CDS
AATGGAGTTGATAACTTTAG+TGG 0.409161 4.1:+34170698 MS.gene056372:CDS
CTGTCAAACCTATCCATTTG+TGG 0.418494 4.1:-34172468 None:intergenic
TGCAAAGATTACTGCTAAGT+TGG 0.422853 4.1:+34170958 MS.gene056372:CDS
CTAATTGTGTGAGAGTGGTT+AGG 0.441187 4.1:-34171016 None:intergenic
GGAATTATGTTAATAGAGAA+AGG 0.442025 4.1:+34171206 MS.gene056372:CDS
ATTTACAAATTGATGCAAAC+TGG 0.443341 4.1:-34171965 None:intergenic
CACCACCTATGTCTAGTAGT+AGG 0.443756 4.1:-34171640 None:intergenic
CACGTGCCTTAGTTGTCAAT+TGG 0.446141 4.1:-34172797 None:intergenic
AATAGAGAAAGGCGTATCAT+TGG 0.465390 4.1:+34171217 MS.gene056372:CDS
CTAGACATAGGTGGTGTTTG+TGG 0.469131 4.1:+34171647 MS.gene056372:CDS
CCATTGCGAGTCACGCGTAA+AGG 0.473557 4.1:+34172693 MS.gene056372:CDS
TTGAATAACCTTGACTTAGT+GGG 0.483800 4.1:-34171040 None:intergenic
CACCTCCGCTACAGATGAAA+AGG 0.484298 4.1:-34170756 None:intergenic
CACTAAGTCAAGGTTATTCA+AGG 0.496285 4.1:+34171042 MS.gene056372:CDS
CAATCAACTTTACTTGGATG+TGG 0.499442 4.1:+34171481 MS.gene056372:CDS
GCAAAGATTACTGCTAAGTT+GGG 0.503061 4.1:+34170959 MS.gene056372:CDS
ACACCAACAAATGCAGCAAA+AGG 0.504617 4.1:-34171445 None:intergenic
AGTAGGGAATACTGATTGAA+TGG 0.504923 4.1:-34171623 None:intergenic
GCGTGACTCGCAATGGACTA+CGG 0.506171 4.1:-34172686 None:intergenic
GACAGAGTTGAAGCCACAAA+TGG 0.507415 4.1:+34172455 MS.gene056372:CDS
AGAGAAGGAAATCATGTGTC+GGG 0.510956 4.1:+34170887 MS.gene056372:CDS
TATTATCACTGACCAATGTA+AGG 0.514760 4.1:+34171588 MS.gene056372:CDS
GAAAGGCGTATCATTGGAAA+AGG 0.517095 4.1:+34171223 MS.gene056372:CDS
ACATCAAAGAAAGGAGAGGA+TGG 0.519853 4.1:+34170833 MS.gene056372:CDS
TGCTATTTACCACGTGTTGG+AGG 0.524536 4.1:+34172197 MS.gene056372:CDS
TTTCTATGAGGTTGCTGAAG+TGG 0.529465 4.1:+34172503 MS.gene056372:CDS
TTGAGCTGGAACATAATGAT+TGG 0.533395 4.1:+34171809 MS.gene056372:CDS
GAAAAGGACATCCCTGATGA+AGG 0.537910 4.1:-34170740 None:intergenic
TTGTGCAGAGAAGCAGGAAG+CGG 0.537951 4.1:+34172757 MS.gene056372:CDS
ATTTGGCCTCGAGTCAGTGT+TGG 0.539381 4.1:-34172625 None:intergenic
AAGATTAGAACATCAAAGAA+AGG 0.539533 4.1:+34170824 MS.gene056372:CDS
CCTTGCTATTTACCACGTGT+TGG 0.542509 4.1:+34172194 MS.gene056372:CDS
TCATGGCTTCGTTGTATGCA+AGG 0.543000 4.1:+34171550 MS.gene056372:CDS
CAGACTATTGTTAGAGACGC+TGG 0.544566 4.1:+34171145 MS.gene056372:CDS
ATCCAAATCAATGATGAAGC+GGG 0.553848 4.1:+34171100 MS.gene056372:CDS
GGGTGACCAATTGACAACTA+AGG 0.555202 4.1:+34172791 MS.gene056372:CDS
GGCCAAATAGAATTATTCGA+AGG 0.555904 4.1:+34172640 MS.gene056372:CDS
AACATAATGATTGGCTGAGT+GGG 0.556371 4.1:+34171818 MS.gene056372:CDS
CCTTTACGCGTGACTCGCAA+TGG 0.556393 4.1:-34172693 None:intergenic
TGGACACAACAATTCCTTGT+GGG 0.558651 4.1:+34172049 MS.gene056372:CDS
GTACAATGAGCGCCATAGAT+GGG 0.559433 4.1:+34171843 MS.gene056372:CDS
ATGCAAGGGAAGGCTCCTGC+AGG 0.564052 4.1:+34171565 MS.gene056372:CDS
TGTTACCTCCTTAAATATCG+TGG 0.565341 4.1:+34172164 MS.gene056372:CDS
GCCAAATAGAATTATTCGAA+GGG 0.566470 4.1:+34172641 MS.gene056372:CDS
ATCATTGGAAAAGGAGGAGA+CGG 0.566514 4.1:+34171232 MS.gene056372:CDS
ACCACCTATGTCTAGTAGTA+GGG 0.566962 4.1:-34171639 None:intergenic
AGTTGAAGCCACAAATGGAT+AGG 0.568044 4.1:+34172460 MS.gene056372:CDS
AGCATTCACAACTAACATCA+TGG 0.572632 4.1:-34172281 None:intergenic
CATCAAAGAAAGGAGAGGAT+GGG 0.575239 4.1:+34170834 MS.gene056372:CDS
ACCTCCTTAAATATCGTGGA+AGG 0.586701 4.1:+34172168 MS.gene056372:CDS
ATGCTATCTTGCTCAAGAGA+AGG 0.587758 4.1:+34170872 MS.gene056372:CDS
AAGAGAAGGAAATCATGTGT+CGG 0.591467 4.1:+34170886 MS.gene056372:CDS
GGAAGTGTCTTCAATGTACA+TGG 0.592537 4.1:-34170914 None:intergenic
CTTGTGCAAGCATTTCTATG+AGG 0.596711 4.1:+34172491 MS.gene056372:CDS
TGGTCAGTGATAATACCTGC+AGG 0.597873 4.1:-34171580 None:intergenic
TGGGGCTAATTGTGTGAGAG+TGG 0.603064 4.1:-34171021 None:intergenic
TGAATAACCTTGACTTAGTG+GGG 0.603118 4.1:-34171039 None:intergenic
TCAATGATGAAGCGGGCGTG+AGG 0.603491 4.1:+34171107 MS.gene056372:CDS
AATCCAAATCAATGATGAAG+CGG 0.605155 4.1:+34171099 MS.gene056372:CDS
ACCTTCCACGATATTTAAGG+AGG 0.607235 4.1:-34172169 None:intergenic
CAAGTAAAGTTGATTGACCA+TGG 0.609756 4.1:-34171474 None:intergenic
TATTCCCTACTACTAGACAT+AGG 0.615682 4.1:+34171635 MS.gene056372:CDS
CATGGCTTCGTTGTATGCAA+GGG 0.615709 4.1:+34171551 MS.gene056372:CDS
CTTACTAAAACATACTTCAG+TGG 0.627472 4.1:-34172378 None:intergenic
ATCGATGAGCTTGACCCACA+AGG 0.628232 4.1:-34172063 None:intergenic
TCTGGTTTCAGCTGAAGACA+CGG 0.628431 4.1:+34171504 MS.gene056372:CDS
CCAACACGTGGTAAATAGCA+AGG 0.628598 4.1:-34172194 None:intergenic
GCTTCGTTGTATGCAAGGGA+AGG 0.629895 4.1:+34171555 MS.gene056372:CDS
AGGCGTATCATTGGAAAAGG+AGG 0.630655 4.1:+34171226 MS.gene056372:CDS
TACTCATGCGAAGTTTAAGG+AGG 0.641837 4.1:+34172110 MS.gene056372:CDS
GAAGTTTAAGGAGGTTCAAG+CGG 0.643158 4.1:+34172119 MS.gene056372:CDS
TAGAACATCAAAGAAAGGAG+AGG 0.643941 4.1:+34170829 MS.gene056372:CDS
ATGGACACAACAATTCCTTG+TGG 0.653497 4.1:+34172048 MS.gene056372:CDS
GAACATAATGATTGGCTGAG+TGG 0.655057 4.1:+34171817 MS.gene056372:CDS
CAATTAGCCCCACTAAGTCA+AGG 0.658920 4.1:+34171032 MS.gene056372:CDS
CAAGTTGCGAGCCTTCATCA+GGG 0.660640 4.1:+34170729 MS.gene056372:CDS
GAAGTTCCAACACTGACTCG+AGG 0.694071 4.1:+34172619 MS.gene056372:CDS
TTTGTTGGTGTGAATCACCA+TGG 0.705691 4.1:+34171457 MS.gene056372:CDS
ACTATTGTTAGAGACGCTGG+AGG 0.710750 4.1:+34171148 MS.gene056372:CDS
TTAACATCTCCTCCAACACG+TGG 0.712095 4.1:-34172206 None:intergenic
TCCCTACTACTAGACATAGG+TGG 0.718251 4.1:+34171638 MS.gene056372:CDS
CTGGTATGTCGACAACACAA+CGG 0.726317 4.1:+34171896 MS.gene056372:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGATTTTTTCAACATAAT+TGG - chr4.1:34171871-34171890 None:intergenic 15.0%
!!! TATGTTGAAAAAATCTTTTT+GGG + chr4.1:34171873-34171892 MS.gene056372:CDS 15.0%
!!! TTATGTTGAAAAAATCTTTT+TGG + chr4.1:34171872-34171891 MS.gene056372:CDS 15.0%
!! AGAAAAAGAAGATAAAACTA+TGG + chr4.1:34172530-34172549 MS.gene056372:CDS 20.0%
!! TTTCTTCTATGATATAGATT+TGG + chr4.1:34171303-34171322 MS.gene056372:CDS 20.0%
!!! AAAAGGCATATCATATTTAT+TGG - chr4.1:34171431-34171450 None:intergenic 20.0%
!!! AAAGAATATGCTTCTAAAAA+GGG + chr4.1:34170791-34170810 MS.gene056372:CDS 20.0%
!!! TAAAGAATATGCTTCTAAAA+AGG + chr4.1:34170790-34170809 MS.gene056372:CDS 20.0%
! AAGATTAGAACATCAAAGAA+AGG + chr4.1:34170824-34170843 MS.gene056372:CDS 25.0%
! ATTTACAAATTGATGCAAAC+TGG - chr4.1:34171968-34171987 None:intergenic 25.0%
! GATGGAGCAAAAATATTAAA+AGG + chr4.1:34172400-34172419 MS.gene056372:CDS 25.0%
! GGAATTATGTTAATAGAGAA+AGG + chr4.1:34171206-34171225 MS.gene056372:CDS 25.0%
! TCACAAACATCAAAATTGTT+TGG - chr4.1:34170659-34170678 None:intergenic 25.0%
!! TTTTGTTAACAAATTTGAGC+TGG + chr4.1:34171795-34171814 MS.gene056372:CDS 25.0%
!!! AACAATTTTGATGTTTGTGA+TGG + chr4.1:34170659-34170678 MS.gene056372:CDS 25.0%
!!! TGAAGTATGTTTTAGTAAGA+TGG + chr4.1:34172382-34172401 MS.gene056372:CDS 25.0%
AATCCAAATCAATGATGAAG+CGG + chr4.1:34171099-34171118 MS.gene056372:CDS 30.0%
AGTTGGGAAAAGATGAATTA+TGG + chr4.1:34170975-34170994 MS.gene056372:CDS 30.0%
ATATACTCATGCGAAGTTTA+AGG + chr4.1:34172107-34172126 MS.gene056372:CDS 30.0%
CACGATCAAAAGAAACTTTA+AGG - chr4.1:34172257-34172276 None:intergenic 30.0%
CATCTGAATTCGAAGAAAAA+TGG + chr4.1:34171767-34171786 MS.gene056372:CDS 30.0%
CTTACTAAAACATACTTCAG+TGG - chr4.1:34172381-34172400 None:intergenic 30.0%
GCCAAATAGAATTATTCGAA+GGG + chr4.1:34172641-34172660 MS.gene056372:CDS 30.0%
TATTATCACTGACCAATGTA+AGG + chr4.1:34171588-34171607 MS.gene056372:CDS 30.0%
TCCCTTCGAATAATTCTATT+TGG - chr4.1:34172645-34172664 None:intergenic 30.0%
TTGAATAACCTTGACTTAGT+GGG - chr4.1:34171043-34171062 None:intergenic 30.0%
! AGAGCTGCATATGAATATTT+TGG + chr4.1:34171373-34171392 MS.gene056372:CDS 30.0%
! AGCTGCATATGAATATTTTG+GGG + chr4.1:34171375-34171394 MS.gene056372:CDS 30.0%
! CATATCTACTAAGCTTTTCA+GGG - chr4.1:34171690-34171709 None:intergenic 30.0%
! CATTTTGTGAAAGAGATGTT+AGG + chr4.1:34171185-34171204 MS.gene056372:CDS 30.0%
! CATTTTTCTTCGAATTCAGA+TGG - chr4.1:34171769-34171788 None:intergenic 30.0%
! GAGCTGCATATGAATATTTT+GGG + chr4.1:34171374-34171393 MS.gene056372:CDS 30.0%
! GATAATTTTCTCTTTGTTCG+AGG - chr4.1:34172723-34172742 None:intergenic 30.0%
! TGAATTCATGAAGTGTTTCA+TGG - chr4.1:34172560-34172579 None:intergenic 30.0%
!! AAATCTAGCTTGCTATGTTT+GGG + chr4.1:34172579-34172598 MS.gene056372:CDS 30.0%
!! AATGGAGTTGATAACTTTAG+TGG + chr4.1:34170698-34170717 MS.gene056372:CDS 30.0%
!! CTTCTAAAAAGGGTTTTGAT+TGG + chr4.1:34170801-34170820 MS.gene056372:CDS 30.0%
!! TGAAGCTGATTTTCAATCAA+TGG + chr4.1:34172029-34172048 MS.gene056372:CDS 30.0%
!! TTGAAAAAATCTTTTTGGGC+TGG + chr4.1:34171877-34171896 MS.gene056372:CDS 30.0%
AACTTAGCAGTAATCTTTGC+AGG - chr4.1:34170959-34170978 None:intergenic 35.0%
AAGAGAAGGAAATCATGTGT+CGG + chr4.1:34170886-34170905 MS.gene056372:CDS 35.0%
AGCATACATGCTTTCTTTGA+TGG + chr4.1:34171925-34171944 MS.gene056372:CDS 35.0%
AGCATTCACAACTAACATCA+TGG - chr4.1:34172284-34172303 None:intergenic 35.0%
ATCCAAATCAATGATGAAGC+GGG + chr4.1:34171100-34171119 MS.gene056372:CDS 35.0%
CAATCAACTTTACTTGGATG+TGG + chr4.1:34171481-34171500 MS.gene056372:CDS 35.0%
CACTAAGTCAAGGTTATTCA+AGG + chr4.1:34171042-34171061 MS.gene056372:CDS 35.0%
CATGGTCAATCAACTTTACT+TGG + chr4.1:34171475-34171494 MS.gene056372:CDS 35.0%
CCTGAAAAGCTTAGTAGATA+TGG + chr4.1:34171688-34171707 MS.gene056372:CDS 35.0%
CTTGAATAACCTTGACTTAG+TGG - chr4.1:34171044-34171063 None:intergenic 35.0%
GCAAAGATTACTGCTAAGTT+GGG + chr4.1:34170959-34170978 MS.gene056372:CDS 35.0%
GGCCAAATAGAATTATTCGA+AGG + chr4.1:34172640-34172659 MS.gene056372:CDS 35.0%
GTTACCTTCCACGATATTTA+AGG - chr4.1:34172175-34172194 None:intergenic 35.0%
TAGAACATCAAAGAAAGGAG+AGG + chr4.1:34170829-34170848 MS.gene056372:CDS 35.0%
TATTCCCTACTACTAGACAT+AGG + chr4.1:34171635-34171654 MS.gene056372:CDS 35.0%
TCGCATGAGTATATTCTTCT+TGG - chr4.1:34172101-34172120 None:intergenic 35.0%
TGAATAACCTTGACTTAGTG+GGG - chr4.1:34171042-34171061 None:intergenic 35.0%
TGCAAAGATTACTGCTAAGT+TGG + chr4.1:34170958-34170977 MS.gene056372:CDS 35.0%
TGTTACCTCCTTAAATATCG+TGG + chr4.1:34172164-34172183 MS.gene056372:CDS 35.0%
TTGAGCTGGAACATAATGAT+TGG + chr4.1:34171809-34171828 MS.gene056372:CDS 35.0%
! AACATAATGATTGGCTGAGT+GGG + chr4.1:34171818-34171837 MS.gene056372:CDS 35.0%
! AATAGAGAAAGGCGTATCAT+TGG + chr4.1:34171217-34171236 MS.gene056372:CDS 35.0%
! AGTAGGGAATACTGATTGAA+TGG - chr4.1:34171626-34171645 None:intergenic 35.0%
! CAAGTAAAGTTGATTGACCA+TGG - chr4.1:34171477-34171496 None:intergenic 35.0%
! CCATATCTACTAAGCTTTTC+AGG - chr4.1:34171691-34171710 None:intergenic 35.0%
! TCATGTACGAAACGTGTTTT+GGG + chr4.1:34171336-34171355 MS.gene056372:CDS 35.0%
! TGCTCATTGTTTGAGTTTAG+AGG + chr4.1:34172300-34172319 MS.gene056372:CDS 35.0%
! TTCATGTACGAAACGTGTTT+TGG + chr4.1:34171335-34171354 MS.gene056372:CDS 35.0%
!! ACTAAGCTTTTCAGGGATTT+TGG - chr4.1:34171683-34171702 None:intergenic 35.0%
!! CAAATCTAGCTTGCTATGTT+TGG + chr4.1:34172578-34172597 MS.gene056372:CDS 35.0%
!! CATTTTGCATAGCCTTACAT+TGG - chr4.1:34171603-34171622 None:intergenic 35.0%
!!! CAATTGGTCACCCTTTTTTT+TGG - chr4.1:34172784-34172803 None:intergenic 35.0%
!!! GATGTTTGTGATGGTTTTGA+TGG + chr4.1:34170668-34170687 MS.gene056372:CDS 35.0%
!!! TTGTGTGGCTTTTTCAATCA+TGG + chr4.1:34171533-34171552 MS.gene056372:CDS 35.0%
AAAAAGTTGTGCAGAGAAGC+AGG + chr4.1:34172751-34172770 MS.gene056372:CDS 40.0%
AACTTTACTTGGATGTGGTC+TGG + chr4.1:34171486-34171505 MS.gene056372:CDS 40.0%
ACACCAACAAATGCAGCAAA+AGG - chr4.1:34171448-34171467 None:intergenic 40.0%
ACATAATTGGCACCCATCTA+TGG - chr4.1:34171858-34171877 None:intergenic 40.0%
ACATCAAAGAAAGGAGAGGA+TGG + chr4.1:34170833-34170852 MS.gene056372:CDS 40.0%
ACCACCTATGTCTAGTAGTA+GGG - chr4.1:34171642-34171661 None:intergenic 40.0%
ACCTCCTTAAATATCGTGGA+AGG + chr4.1:34172168-34172187 MS.gene056372:CDS 40.0%
ACCTTCCACGATATTTAAGG+AGG - chr4.1:34172172-34172191 None:intergenic 40.0%
AGAGAAGGAAATCATGTGTC+GGG + chr4.1:34170887-34170906 MS.gene056372:CDS 40.0%
AGGAAGCGGACCAAAAAAAA+GGG + chr4.1:34172771-34172790 MS.gene056372:CDS 40.0%
AGTTGAAGCCACAAATGGAT+AGG + chr4.1:34172460-34172479 MS.gene056372:CDS 40.0%
ATCATTGGAAAAGGAGGAGA+CGG + chr4.1:34171232-34171251 MS.gene056372:CDS 40.0%
ATGCTATCTTGCTCAAGAGA+AGG + chr4.1:34170872-34170891 MS.gene056372:CDS 40.0%
ATGGACACAACAATTCCTTG+TGG + chr4.1:34172048-34172067 MS.gene056372:CDS 40.0%
CATCAAAGAAAGGAGAGGAT+GGG + chr4.1:34170834-34170853 MS.gene056372:CDS 40.0%
CTAATTGTGTGAGAGTGGTT+AGG - chr4.1:34171019-34171038 None:intergenic 40.0%
CTGTCAAACCTATCCATTTG+TGG - chr4.1:34172471-34172490 None:intergenic 40.0%
CTTGTGCAAGCATTTCTATG+AGG + chr4.1:34172491-34172510 MS.gene056372:CDS 40.0%
GAAGTTTAAGGAGGTTCAAG+CGG + chr4.1:34172119-34172138 MS.gene056372:CDS 40.0%
GTGTTGGAGGAGATGTTAAT+TGG + chr4.1:34172210-34172229 MS.gene056372:CDS 40.0%
TACTCATGCGAAGTTTAAGG+AGG + chr4.1:34172110-34172129 MS.gene056372:CDS 40.0%
TGAAGACACTTCCGACTAAA+AGG + chr4.1:34170924-34170943 MS.gene056372:CDS 40.0%
TGGACACAACAATTCCTTGT+GGG + chr4.1:34172049-34172068 MS.gene056372:CDS 40.0%
TTTCTATGAGGTTGCTGAAG+TGG + chr4.1:34172503-34172522 MS.gene056372:CDS 40.0%
TTTGTTGGTGTGAATCACCA+TGG + chr4.1:34171457-34171476 MS.gene056372:CDS 40.0%
! ATGCCTTTTGCTGCATTTGT+TGG + chr4.1:34171442-34171461 MS.gene056372:CDS 40.0%
! GAAAGGCGTATCATTGGAAA+AGG + chr4.1:34171223-34171242 MS.gene056372:CDS 40.0%
! GAACATAATGATTGGCTGAG+TGG + chr4.1:34171817-34171836 MS.gene056372:CDS 40.0%
! GATGTCCTTTTCATCTGTAG+CGG + chr4.1:34170751-34170770 MS.gene056372:CDS 40.0%
! GGACAGCTTTTCCTTTTAGT+CGG - chr4.1:34170938-34170957 None:intergenic 40.0%
!! GGAAGTGTCTTCAATGTACA+TGG - chr4.1:34170917-34170936 None:intergenic 40.0%
!!! GGTTTTGATGGTGAAGCTAA+TGG + chr4.1:34170680-34170699 MS.gene056372:CDS 40.0%
ACTATTGTTAGAGACGCTGG+AGG + chr4.1:34171148-34171167 MS.gene056372:CDS 45.0%
AGGCGTATCATTGGAAAAGG+AGG + chr4.1:34171226-34171245 MS.gene056372:CDS 45.0%
CAATTAGCCCCACTAAGTCA+AGG + chr4.1:34171032-34171051 MS.gene056372:CDS 45.0%
CACCACCTATGTCTAGTAGT+AGG - chr4.1:34171643-34171662 None:intergenic 45.0%
CACGTGCCTTAGTTGTCAAT+TGG - chr4.1:34172800-34172819 None:intergenic 45.0%
CAGACTATTGTTAGAGACGC+TGG + chr4.1:34171145-34171164 MS.gene056372:CDS 45.0%
CAGGAAGCGGACCAAAAAAA+AGG + chr4.1:34172770-34172789 MS.gene056372:CDS 45.0%
CATGGCTTCGTTGTATGCAA+GGG + chr4.1:34171551-34171570 MS.gene056372:CDS 45.0%
CCAACACGTGGTAAATAGCA+AGG - chr4.1:34172197-34172216 None:intergenic 45.0%
CTAGACATAGGTGGTGTTTG+TGG + chr4.1:34171647-34171666 MS.gene056372:CDS 45.0%
CTGGTATGTCGACAACACAA+CGG + chr4.1:34171896-34171915 MS.gene056372:CDS 45.0%
GAAAAGGACATCCCTGATGA+AGG - chr4.1:34170743-34170762 None:intergenic 45.0%
GACAGAGTTGAAGCCACAAA+TGG + chr4.1:34172455-34172474 MS.gene056372:CDS 45.0%
GGGTGACCAATTGACAACTA+AGG + chr4.1:34172791-34172810 MS.gene056372:CDS 45.0%
GTACAATGAGCGCCATAGAT+GGG + chr4.1:34171843-34171862 MS.gene056372:CDS 45.0%
TCATGGCTTCGTTGTATGCA+AGG + chr4.1:34171550-34171569 MS.gene056372:CDS 45.0%
TCCCTACTACTAGACATAGG+TGG + chr4.1:34171638-34171657 MS.gene056372:CDS 45.0%
TGGTCAGTGATAATACCTGC+AGG - chr4.1:34171583-34171602 None:intergenic 45.0%
TGTACAATGAGCGCCATAGA+TGG + chr4.1:34171842-34171861 MS.gene056372:CDS 45.0%
TTAACATCTCCTCCAACACG+TGG - chr4.1:34172209-34172228 None:intergenic 45.0%
! AAGACACGGAGTCTTTTGTG+TGG + chr4.1:34171518-34171537 MS.gene056372:CDS 45.0%
! TCTGGTTTCAGCTGAAGACA+CGG + chr4.1:34171504-34171523 MS.gene056372:CDS 45.0%
! TGCTATTTACCACGTGTTGG+AGG + chr4.1:34172197-34172216 MS.gene056372:CDS 45.0%
!! CCTTGCTATTTACCACGTGT+TGG + chr4.1:34172194-34172213 MS.gene056372:CDS 45.0%
ATCGATGAGCTTGACCCACA+AGG - chr4.1:34172066-34172085 None:intergenic 50.0%
ATTTGGCCTCGAGTCAGTGT+TGG - chr4.1:34172628-34172647 None:intergenic 50.0%
CAAGTTGCGAGCCTTCATCA+GGG + chr4.1:34170729-34170748 MS.gene056372:CDS 50.0%
CACCTCCGCTACAGATGAAA+AGG - chr4.1:34170759-34170778 None:intergenic 50.0%
GCTTCGTTGTATGCAAGGGA+AGG + chr4.1:34171555-34171574 MS.gene056372:CDS 50.0%
TGGGGCTAATTGTGTGAGAG+TGG - chr4.1:34171024-34171043 None:intergenic 50.0%
TTGTGCAGAGAAGCAGGAAG+CGG + chr4.1:34172757-34172776 MS.gene056372:CDS 50.0%
! CGCCCGCTTCATCATTGATT+TGG - chr4.1:34171105-34171124 None:intergenic 50.0%
! GTCCTTTTCATCTGTAGCGG+AGG + chr4.1:34170754-34170773 MS.gene056372:CDS 50.0%
!! GAAGTTCCAACACTGACTCG+AGG + chr4.1:34172619-34172638 MS.gene056372:CDS 50.0%
CCATTGCGAGTCACGCGTAA+AGG + chr4.1:34172693-34172712 MS.gene056372:CDS 55.0%
CCTTTACGCGTGACTCGCAA+TGG - chr4.1:34172696-34172715 None:intergenic 55.0%
GCAAGTTGCGAGCCTTCATC+AGG + chr4.1:34170728-34170747 MS.gene056372:CDS 55.0%
TCAATGATGAAGCGGGCGTG+AGG + chr4.1:34171107-34171126 MS.gene056372:CDS 55.0%
!! GCGTGACTCGCAATGGACTA+CGG - chr4.1:34172689-34172708 None:intergenic 55.0%
ATGCAAGGGAAGGCTCCTGC+AGG + chr4.1:34171565-34171584 MS.gene056372:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 34170647 34172857 34170647 ID=MS.gene056372
chr4.1 mRNA 34170647 34172857 34170647 ID=MS.gene056372.t1;Parent=MS.gene056372
chr4.1 exon 34170647 34172857 34170647 ID=MS.gene056372.t1.exon1;Parent=MS.gene056372.t1
chr4.1 CDS 34170647 34172857 34170647 ID=cds.MS.gene056372.t1;Parent=MS.gene056372.t1
Gene Sequence

>MS.gene056372

ATGGATAGTCCAAACAATTTTGATGTTTGTGATGGTTTTGATGGTGAAGCTAATGGAGTTGATAACTTTAGTGGTGAACAAGCAAGTTGCGAGCCTTCATCAGGGATGTCCTTTTCATCTGTAGCGGAGGTGAAATCATTTTATAAAGAATATGCTTCTAAAAAGGGTTTTGATTGGAAGATTAGAACATCAAAGAAAGGAGAGGATGGGAATCTCAAGTACTTCATGCTATCTTGCTCAAGAGAAGGAAATCATGTGTCGGGAGTACCATGTACATTGAAGACACTTCCGACTAAAAGGAAAAGCTGTCCTGCAAAGATTACTGCTAAGTTGGGAAAAGATGAATTATGGTATATTAAAAAGTTTGAACCTAACCACTCTCACACAATTAGCCCCACTAAGTCAAGGTTATTCAAGGCAAACAAAAATATGAACATGCATGCAAAGAGAACAATCCAAATCAATGATGAAGCGGGCGTGAGGATCAACAAGACTTTTCAGACTATTGTTAGAGACGCTGGAGGTTATGAAAATGTAGCATTTTGTGAAAGAGATGTTAGGAATTATGTTAATAGAGAAAGGCGTATCATTGGAAAAGGAGGAGACGGCAAAGCATTGCTAAATTATTTTTGTCGAATGAGAGAGCAAAATACAAATTTCTTCTATGATATAGATTTGGATGAAGATTTTCATGTACGAAACGTGTTTTGGGCTGATGCAAGAAGCAGAGCTGCATATGAATATTTTGGGGATGTTGTGACTTTCGATACAACGTATTTGACCAATAAATATGATATGCCTTTTGCTGCATTTGTTGGTGTGAATCACCATGGTCAATCAACTTTACTTGGATGTGGTCTGGTTTCAGCTGAAGACACGGAGTCTTTTGTGTGGCTTTTTCAATCATGGCTTCGTTGTATGCAAGGGAAGGCTCCTGCAGGTATTATCACTGACCAATGTAAGGCTATGCAAAATGCCATTCAATCAGTATTCCCTACTACTAGACATAGGTGGTGTTTGTGGCACATAATGACCAAAATCCCTGAAAAGCTTAGTAGATATGGTAAATATAAGAATATTAAGTTTGCAATGAAAGAAGCAGTTTATGATTCACTTACACCATCTGAATTCGAAGAAAAATGGTGTTATTTTGTTAACAAATTTGAGCTGGAACATAATGATTGGCTGAGTGGGTTGTACAATGAGCGCCATAGATGGGTGCCAATTATGTTGAAAAAATCTTTTTGGGCTGGTATGTCGACAACACAACGGAGTGAGAGCATACATGCTTTCTTTGATGGATATATCAATTCGACAACCAGTTTGCATCAATTTGTAAATCAATATGATAATGCTCTCAAAAGTAGAGCAGAAAAAGAATTTGAAGCTGATTTTCAATCAATGGACACAACAATTCCTTGTGGGTCAAGCTCATCGATCGAAAAGCAATTCCAAGAAGAATATACTCATGCGAAGTTTAAGGAGGTTCAAGCGGAATTCAGAGCTAAAATGAATTGTGTTACCTCCTTAAATATCGTGGAAGGTAACCTTGCTATTTACCACGTGTTGGAGGAGATGTTAATTGGAGATAGAAGAAAAGATCGTATCCTTAAAGTTTCTTTTGATCGTGACAGCCATGATGTTAGTTGTGAATGCTCATTGTTTGAGTTTAGAGGTATTGTATGTCGTCATGTGCTATCTGTGTGTGCTCAAGAAAGAGTTAAAAACATGCCACTGAAGTATGTTTTAGTAAGATGGAGCAAAAATATTAAAAGGAAGCATTCATATATTAAGAGTAGCTATAATGTGACAGAGTTGAAGCCACAAATGGATAGGTTTGACAGCTTGTGCAAGCATTTCTATGAGGTTGCTGAAGTGGCTGCAGAAAAAGAAGATAAAACTATGGCTCTCCATGAAACACTTCATGAATTCAAATCTAGCTTGCTATGTTTGGGTGCTACAAGTAACAACAGAAGTTCCAACACTGACTCGAGGCCAAATAGAATTATTCGAAGGGATGAAGATGCGATTATTGAAATCCGTAGTCCATTGCGAGTCACGCGTAAAGGTCGTCCTCGAACAAAGAGAAAATTATCTTTAGTTGAAAAAGTTGTGCAGAGAAGCAGGAAGCGGACCAAAAAAAAGGGTGACCAATTGACAACTAAGGCACGTGATTATTTTCATAATATATGCATTAAAATGAAGTATTAA
Protein sequence

>MS.gene056372.t1

MDSPNNFDVCDGFDGEANGVDNFSGEQASCEPSSGMSFSSVAEVKSFYKEYASKKGFDWKIRTSKKGEDGNLKYFMLSCSREGNHVSGVPCTLKTLPTKRKSCPAKITAKLGKDELWYIKKFEPNHSHTISPTKSRLFKANKNMNMHAKRTIQINDEAGVRINKTFQTIVRDAGGYENVAFCERDVRNYVNRERRIIGKGGDGKALLNYFCRMREQNTNFFYDIDLDEDFHVRNVFWADARSRAAYEYFGDVVTFDTTYLTNKYDMPFAAFVGVNHHGQSTLLGCGLVSAEDTESFVWLFQSWLRCMQGKAPAGIITDQCKAMQNAIQSVFPTTRHRWCLWHIMTKIPEKLSRYGKYKNIKFAMKEAVYDSLTPSEFEEKWCYFVNKFELEHNDWLSGLYNERHRWVPIMLKKSFWAGMSTTQRSESIHAFFDGYINSTTSLHQFVNQYDNALKSRAEKEFEADFQSMDTTIPCGSSSSIEKQFQEEYTHAKFKEVQAEFRAKMNCVTSLNIVEGNLAIYHVLEEMLIGDRRKDRILKVSFDRDSHDVSCECSLFEFRGIVCRHVLSVCAQERVKNMPLKYVLVRWSKNIKRKHSYIKSSYNVTELKPQMDRFDSLCKHFYEVAEVAAEKEDKTMALHETLHEFKSSLLCLGATSNNRSSNTDSRPNRIIRRDEDAIIEIRSPLRVTRKGRPRTKRKLSLVEKVVQRSRKRTKKKGDQLTTKARDYFHNICIKMKY