AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056472

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056472.t1 MTR_8g069935 62.216 352 85 5 69 410 150 463 1.02e-127 376
MS.gene056472.t1 MTR_8g069935 90.123 162 15 1 1 162 1 161 1.55e-45 163
MS.gene056472.t1 MTR_2g024380 43.301 515 142 10 1 410 1 470 5.16e-111 334
MS.gene056472.t1 MTR_8g067635 43.571 420 172 9 33 409 138 535 4.85e-84 266
MS.gene056472.t1 MTR_8g067635 60.795 176 54 3 1 161 1 176 1.48e-38 145
MS.gene056472.t1 MTR_8g067650 46.133 375 154 6 69 409 159 519 8.17e-81 258
MS.gene056472.t1 MTR_8g067650 61.143 175 48 3 1 161 1 169 8.75e-46 165
MS.gene056472.t1 MTR_6g053200 40.525 343 128 7 117 410 221 536 1.64e-62 210
MS.gene056472.t1 MTR_6g053200 55.030 169 65 3 1 162 1 165 1.46e-30 123
MS.gene056472.t1 MTR_2g024350 44.493 227 64 3 246 410 1 227 3.63e-52 174
MS.gene056472.t1 MTR_2g033110 54.545 165 68 4 1 162 1 161 1.45e-31 119
MS.gene056472.t1 MTR_7g105000 42.654 211 99 5 69 273 146 340 1.03e-30 121
MS.gene056472.t1 MTR_7g105000 55.556 162 67 1 1 162 1 157 2.49e-22 97.8
MS.gene056472.t1 MTR_7g104980 59.000 100 40 1 274 372 18 117 2.45e-28 111
MS.gene056472.t1 MTR_1g099600 51.020 98 47 1 230 327 163 259 1.73e-27 111
MS.gene056472.t1 MTR_7g079040 45.263 95 52 0 232 326 133 227 3.23e-23 99.8
MS.gene056472.t1 MTR_8g028565 44.330 97 52 1 230 326 134 228 4.16e-23 99.8
MS.gene056472.t1 MTR_2g032030 42.857 112 53 2 233 334 238 348 4.49e-21 95.1
MS.gene056472.t1 MTR_2g032030 42.857 112 53 2 233 334 238 348 9.40e-21 94.4
MS.gene056472.t1 MTR_8g098945 53.521 71 33 0 254 324 130 200 1.06e-19 88.6
MS.gene056472.t1 MTR_4g094908 57.895 57 24 0 254 310 267 323 9.80e-18 84.0
MS.gene056472.t1 MTR_4g127040 56.061 66 28 1 249 314 242 306 1.19e-17 83.6
MS.gene056472.t1 MTR_8g087000 50.000 66 33 0 251 316 75 140 1.82e-17 81.6
MS.gene056472.t1 MTR_2g083870 55.556 63 28 0 248 310 75 137 2.02e-17 82.0
MS.gene056472.t1 MTR_3g108960 44.681 94 51 1 224 316 284 377 2.27e-17 83.6
MS.gene056472.t1 MTR_8g087000 50.000 66 33 0 251 316 74 139 2.27e-17 81.6
MS.gene056472.t1 MTR_5g016610 56.140 57 25 0 254 310 200 256 6.46e-17 80.9
MS.gene056472.t1 MTR_7g109600 48.529 68 35 0 243 310 43 110 1.13e-16 79.7
MS.gene056472.t1 MTR_2g016770 45.783 83 42 1 251 330 25 107 1.60e-16 78.2
MS.gene056472.t1 MTR_2g016770 45.783 83 42 1 251 330 33 115 1.61e-16 78.6
MS.gene056472.t1 MTR_1g070455 56.140 57 25 0 254 310 294 350 2.97e-16 80.1
MS.gene056472.t1 MTR_1g009613 45.833 72 35 2 254 321 263 334 6.54e-16 79.0
MS.gene056472.t1 MTR_0117s0040 53.448 58 27 0 254 311 75 132 7.50e-15 74.3
MS.gene056472.t1 MTR_7g011820 40.244 82 48 1 253 334 410 490 4.91e-14 73.9
MS.gene056472.t1 MTR_7g011820 46.377 69 34 3 249 316 224 290 1.68e-12 69.3
MS.gene056472.t1 MTR_7g100510 53.226 62 27 2 249 310 185 244 2.98e-13 71.2
MS.gene056472.t1 MTR_7g100510 37.500 96 45 2 230 311 324 418 6.22e-13 70.5
MS.gene056472.t1 MTR_7g071120 44.898 98 48 4 220 316 136 228 4.08e-13 71.2
MS.gene056472.t1 MTR_3g009470 47.297 74 36 3 251 323 278 349 4.32e-13 71.6
MS.gene056472.t1 MTR_3g009470 42.029 69 38 2 253 320 495 562 2.35e-11 66.2
MS.gene056472.t1 MTR_3g009470 47.297 74 36 3 251 323 278 349 5.25e-13 70.9
MS.gene056472.t1 MTR_8g099350 40.230 87 46 2 228 308 103 189 8.03e-13 69.7
MS.gene056472.t1 MTR_4g052030 54.545 55 13 1 256 310 8 50 9.87e-13 62.8
MS.gene056472.t1 MTR_4g094588 48.276 58 29 1 253 310 178 234 1.46e-12 68.6
MS.gene056472.t1 MTR_5g042920 50.000 62 29 2 249 310 147 206 1.48e-12 69.3
MS.gene056472.t1 MTR_5g042920 44.776 67 35 2 253 318 320 385 2.87e-12 68.6
MS.gene056472.t1 MTR_1g086790 37.363 91 54 3 233 322 139 227 2.15e-12 68.2
MS.gene056472.t1 MTR_4g130900 35.238 105 55 3 218 310 312 415 2.32e-12 68.9
MS.gene056472.t1 MTR_7g080020 37.143 105 50 4 253 356 493 582 2.51e-12 69.3
MS.gene056472.t1 MTR_7g080020 42.683 82 44 3 243 323 270 349 5.61e-12 68.2
MS.gene056472.t1 MTR_4g082580 42.500 80 44 2 253 331 278 356 3.67e-12 67.8
MS.gene056472.t1 MTR_7g109800 37.500 96 57 3 226 320 119 212 4.54e-12 66.6
MS.gene056472.t1 MTR_5g091390 48.276 58 29 1 253 310 157 213 1.12e-11 65.9
MS.gene056472.t1 MTR_4g013260 50.000 66 31 2 246 310 176 240 1.34e-11 66.6
MS.gene056472.t1 MTR_4g013260 43.103 58 32 1 253 310 336 392 6.31e-11 64.3
MS.gene056472.t1 MTR_7g062220 32.394 142 88 4 176 310 40 180 1.68e-11 65.5
MS.gene056472.t1 MTR_3g106060 31.667 120 75 2 253 372 138 250 1.93e-11 64.7
MS.gene056472.t1 MTR_5g067680 46.667 60 30 1 251 308 200 259 2.54e-11 65.1
MS.gene056472.t1 MTR_8g007270 35.849 106 62 5 215 318 117 218 2.56e-11 63.5
MS.gene056472.t1 MTR_3g090860 34.862 109 59 4 231 331 106 210 2.68e-11 64.7
MS.gene056472.t1 MTR_3g031220 48.485 66 31 3 252 316 197 260 3.01e-11 65.1
MS.gene056472.t1 MTR_3g031220 48.485 66 31 3 252 316 197 260 3.24e-11 65.5
MS.gene056472.t1 MTR_4g122530 41.379 58 33 1 253 310 208 264 4.44e-11 64.7
MS.gene056472.t1 MTR_2g033820 44.828 58 31 1 253 310 203 259 6.29e-11 63.9
MS.gene056472.t1 MTR_7g080000 44.068 59 32 1 253 311 432 489 6.60e-11 64.7
MS.gene056472.t1 MTR_5g074400 50.847 59 27 2 252 310 242 298 7.72e-11 64.3
MS.gene056472.t1 MTR_5g043880 38.824 85 50 2 226 310 98 180 9.16e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056472.t1 AT5G52830 51.020 98 47 1 230 327 143 239 6.60e-27 110
MS.gene056472.t1 AT4G01250 35.616 146 88 3 181 326 62 201 9.63e-24 100
MS.gene056472.t1 AT4G23550 44.086 93 52 0 237 329 124 216 6.90e-21 92.8
MS.gene056472.t1 AT5G45050 36.022 186 105 6 233 409 1021 1201 1.66e-20 94.7
MS.gene056472.t1 AT5G45050 36.022 186 105 6 233 409 1132 1312 2.08e-20 94.4
MS.gene056472.t1 AT5G45050 48.315 89 46 0 233 321 1160 1248 2.10e-20 94.4
MS.gene056472.t1 AT1G30650 54.412 68 31 0 254 321 218 285 3.22e-19 89.7
MS.gene056472.t1 AT2G34830 54.412 68 31 0 254 321 216 283 3.25e-19 89.4
MS.gene056472.t1 AT2G34830 54.412 68 31 0 254 321 225 292 3.40e-19 89.4
MS.gene056472.t1 AT4G31550 33.103 145 84 2 194 325 173 317 8.49e-18 84.3
MS.gene056472.t1 AT2G23320 45.977 87 43 2 224 310 215 297 1.21e-17 83.6
MS.gene056472.t1 AT2G24570 50.000 66 33 0 254 319 244 309 1.33e-17 83.6
MS.gene056472.t1 AT4G31550 45.833 72 39 0 254 325 247 318 2.01e-17 83.2
MS.gene056472.t1 AT1G29280 51.515 66 32 0 245 310 66 131 4.07e-17 81.3
MS.gene056472.t1 AT4G24240 47.619 84 39 2 231 310 256 338 7.60e-17 81.6
MS.gene056472.t1 AT5G28650 50.000 66 33 0 254 319 263 328 1.11e-16 80.9
MS.gene056472.t1 AT3G04670 50.000 66 33 0 254 319 263 328 1.58e-16 80.5
MS.gene056472.t1 AT3G58710 53.333 60 28 0 251 310 68 127 2.89e-16 79.0
MS.gene056472.t1 AT3G58710 53.333 60 28 0 251 310 69 128 2.90e-16 79.0
MS.gene056472.t1 AT2G30590 52.632 57 27 0 254 310 314 370 7.63e-15 75.9
MS.gene056472.t1 AT3G01080 43.836 73 39 2 249 321 201 271 4.73e-14 73.2
MS.gene056472.t1 AT3G01080 43.836 73 39 2 249 321 201 271 1.09e-13 72.8
MS.gene056472.t1 AT3G01080 46.552 58 30 1 253 310 344 400 7.46e-13 70.5
MS.gene056472.t1 AT3G01080 43.836 73 39 2 249 321 163 233 1.25e-13 72.4
MS.gene056472.t1 AT3G01080 46.552 58 30 1 253 310 306 362 9.82e-13 69.7
MS.gene056472.t1 AT2G47260 36.207 116 65 4 226 335 148 260 1.41e-13 71.6
MS.gene056472.t1 AT3G62340 38.554 83 50 1 253 335 118 199 2.67e-13 70.1
MS.gene056472.t1 AT2G38470 48.052 77 36 3 234 310 167 239 2.86e-13 71.6
MS.gene056472.t1 AT1G13960 40.230 87 45 3 234 315 205 289 3.57e-13 71.6
MS.gene056472.t1 AT1G13960 46.552 58 30 1 253 310 409 465 2.59e-12 68.9
MS.gene056472.t1 AT2G03340 48.276 58 29 1 253 310 415 471 5.88e-13 70.9
MS.gene056472.t1 AT2G03340 47.619 63 31 2 249 311 246 306 4.79e-12 68.2
MS.gene056472.t1 AT1G13960 43.210 81 39 3 240 315 58 136 9.73e-13 69.3
MS.gene056472.t1 AT1G13960 46.552 58 30 1 253 310 256 312 4.69e-12 67.4
MS.gene056472.t1 AT4G30935 34.906 106 56 3 217 310 283 387 1.09e-12 69.7
MS.gene056472.t1 AT1G64000 36.905 84 49 3 228 310 90 170 2.06e-12 66.2
MS.gene056472.t1 AT5G43290 53.448 58 26 1 253 310 114 170 2.45e-12 67.4
MS.gene056472.t1 AT2G21900 38.202 89 50 2 226 310 78 165 3.50e-12 65.5
MS.gene056472.t1 AT5G56270 49.275 69 32 3 251 318 271 337 3.72e-12 68.6
MS.gene056472.t1 AT5G56270 43.103 58 32 1 253 310 487 543 9.22e-11 64.3
MS.gene056472.t1 AT2G30250 49.123 57 28 1 254 310 329 384 4.33e-12 67.8
MS.gene056472.t1 AT5G41570 38.824 85 49 3 228 310 71 154 4.36e-12 64.7
MS.gene056472.t1 AT4G26440 44.595 74 38 3 251 323 176 247 7.74e-12 67.4
MS.gene056472.t1 AT2G40740 35.870 92 54 2 222 308 139 230 7.98e-12 66.2
MS.gene056472.t1 AT2G40740 35.870 92 54 2 222 308 148 239 1.01e-11 65.9
MS.gene056472.t1 AT2G37260 36.905 84 49 2 230 310 323 405 1.01e-11 66.6
MS.gene056472.t1 AT4G18170 39.744 78 45 2 233 310 153 228 1.14e-11 65.9
MS.gene056472.t1 AT2G44745 36.275 102 61 4 214 313 105 204 1.33e-11 64.3
MS.gene056472.t1 AT2G37260 36.905 84 49 2 230 310 243 325 1.64e-11 65.5
MS.gene056472.t1 AT2G37260 36.905 84 49 2 230 310 241 323 1.76e-11 65.5
MS.gene056472.t1 AT1G29860 46.552 58 30 1 253 310 136 192 2.01e-11 64.7
MS.gene056472.t1 AT1G80840 43.103 58 33 0 253 310 146 203 2.15e-11 64.7
MS.gene056472.t1 AT1G69310 38.372 86 51 2 226 311 121 204 2.27e-11 64.7
MS.gene056472.t1 AT1G69310 38.372 86 51 2 226 311 121 204 2.27e-11 64.7
MS.gene056472.t1 AT1G69310 38.372 86 51 2 226 311 121 204 2.27e-11 64.7
MS.gene056472.t1 AT5G26170 46.032 63 33 1 248 310 108 169 2.73e-11 62.4
MS.gene056472.t1 AT2G04880 47.368 57 29 1 254 310 284 339 3.06e-11 65.5
MS.gene056472.t1 AT2G04880 47.368 57 29 1 254 310 308 363 3.32e-11 65.5
MS.gene056472.t1 AT5G49520 43.103 58 32 1 253 310 221 277 3.80e-11 64.7
MS.gene056472.t1 AT1G55600 46.032 63 32 2 248 310 303 363 3.96e-11 65.1
MS.gene056472.t1 AT4G26640 36.538 104 58 5 252 351 170 269 5.55e-11 64.7
MS.gene056472.t1 AT5G46350 40.278 72 41 2 239 310 170 239 5.86e-11 63.9
MS.gene056472.t1 AT4G26640 36.538 104 58 5 252 351 210 309 6.08e-11 64.7
MS.gene056472.t1 AT5G07100 35.455 110 63 4 252 354 23 131 6.23e-11 62.4
MS.gene056472.t1 AT5G07100 35.455 110 63 4 252 354 23 131 6.23e-11 62.4
MS.gene056472.t1 AT5G07100 35.455 110 63 4 252 354 23 131 6.23e-11 62.4
MS.gene056472.t1 AT5G07100 35.455 110 63 4 252 354 23 131 6.23e-11 62.4
MS.gene056472.t1 AT4G26640 36.538 104 58 5 252 351 138 237 6.63e-11 64.3
MS.gene056472.t1 AT5G07100 42.373 59 33 1 253 311 234 291 7.25e-11 63.5
MS.gene056472.t1 AT4G26640 36.538 104 58 5 252 351 34 133 9.30e-11 63.5
MS.gene056472.t1 AT4G26640 36.538 104 58 5 252 351 34 133 9.30e-11 63.5
MS.gene056472.t1 AT4G26640 36.538 104 58 5 252 351 34 133 9.30e-11 63.5
MS.gene056472.t1 AT4G26640 36.538 104 58 5 252 351 34 133 9.30e-11 63.5

Find 106 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGGTTTAAGTGGGGTAAT+TGG 0.192042 8.3:+32574946 None:intergenic
ACTACAAATGTAGAAGTTTC+TGG 0.203501 8.3:-32572963 MS.gene056472:CDS
TCGGTTTAAGTGGGGTAATT+GGG 0.264814 8.3:+32574947 None:intergenic
GGAAGGGATGGTGGTGCTTG+AGG 0.267804 8.3:+32574807 None:intergenic
TGGAGGTGGAGTTTCAAAAT+TGG 0.270539 8.3:+32575065 None:intergenic
TGGATGTGGAGACCCTTTAA+TGG 0.270856 8.3:+32574019 None:intergenic
TGGGGCTGATTTATTCTCTT+TGG 0.279506 8.3:+32574966 None:intergenic
TATGGAGAAAACCCCATTAA+AGG 0.283476 8.3:-32574032 MS.gene056472:CDS
GAAGGGATGGTGGTGCTTGA+GGG 0.285161 8.3:+32574808 None:intergenic
TGCGATTTCACTGCGGGCAT+GGG 0.316131 8.3:+32574375 None:intergenic
AAACTTGGAATCGCCTAATA+TGG 0.320459 8.3:-32572752 MS.gene056472:CDS
TAGTCTTTGATGACTTGTTT+TGG 0.324581 8.3:+32572818 None:intergenic
CTCCAATGGACAATGATGAT+TGG 0.334060 8.3:-32575143 None:intergenic
GGATGTGGAGACCCTTTAAT+GGG 0.334936 8.3:+32574020 None:intergenic
CAAAATTTAAATCAAGAAAA+AGG 0.335426 8.3:-32574222 MS.gene056472:intron
TTGAGGGGATATGATGTTAT+TGG 0.344141 8.3:+32575026 None:intergenic
GTAGTTGATTGCGCTTTCTC+AGG 0.345960 8.3:+32574302 None:intergenic
ATGAAATCATTCGGTTTAAG+TGG 0.356968 8.3:+32574937 None:intergenic
GGATATGATGTTATTGGTTG+TGG 0.366736 8.3:+32575032 None:intergenic
ACTTATGTCGTGACATATAA+AGG 0.369771 8.3:-32572892 MS.gene056472:CDS
TTGCGATTTCACTGCGGGCA+TGG 0.375796 8.3:+32574374 None:intergenic
TACACTTCATTGTTACGGTT+TGG 0.377794 8.3:+32574409 None:intergenic
AGTTCTCACCGATACGTCTA+TGG 0.382535 8.3:-32572689 MS.gene056472:CDS
AGGGATGGTGGTGCTTGAGG+GGG 0.385352 8.3:+32574810 None:intergenic
GTTGGGGTGGTAGTGATGGT+TGG 0.392907 8.3:+32574844 None:intergenic
GTTGTGGTGGTTAGAGTTGG+AGG 0.394427 8.3:+32575048 None:intergenic
TTTGTTCGAGGTAGTAGAAA+TGG 0.396886 8.3:+32574280 None:intergenic
AAGGGATGGTGGTGCTTGAG+GGG 0.398824 8.3:+32574809 None:intergenic
AACGGCAACATAGCCATATT+AGG 0.400299 8.3:+32572739 None:intergenic
GTTGGGATACTAGTGATAGT+AGG 0.406363 8.3:+32574862 None:intergenic
AAACATGACACCTATGTTAG+AGG 0.407154 8.3:-32572626 MS.gene056472:CDS
TCCAATGGACAATGATGATT+GGG 0.413894 8.3:-32575142 None:intergenic
TTGGTTGTGGTGGTTAGAGT+TGG 0.415176 8.3:+32575045 None:intergenic
CGCTTTCTCAGGGGTTGTTG+TGG 0.415304 8.3:+32574313 None:intergenic
TTGGGATACTAGTGATAGTA+GGG 0.418845 8.3:+32574863 None:intergenic
TTGGGGTGGTAGTGATGGTT+GGG 0.421087 8.3:+32574845 None:intergenic
CTACAAATGTAGAAGTTTCT+GGG 0.423709 8.3:-32572962 MS.gene056472:CDS
TAGAAGTTTCTGGGATTGTT+CGG 0.428728 8.3:-32572953 MS.gene056472:CDS
GGTAATGTGGGAAGGGATGG+TGG 0.443946 8.3:+32574798 None:intergenic
ACTTGAGCCTCGTTGCTCTC+TGG 0.444131 8.3:+32572709 None:intergenic
GATAGTAGGGGTGGGGATAG+TGG 0.445875 8.3:+32574876 None:intergenic
AGTGGTTGGGGTGGTAGTGA+TGG 0.446309 8.3:+32574840 None:intergenic
TCAAGCTCTATGAAATCATT+CGG 0.447306 8.3:+32574928 None:intergenic
ATGGAGAAAACCCCATTAAA+GGG 0.449347 8.3:-32574031 MS.gene056472:CDS
TATGTTAGAGGATCTCTTGA+TGG 0.450163 8.3:-32572614 MS.gene056472:CDS
AAACATACACTTCATTGTTA+CGG 0.454894 8.3:+32574404 None:intergenic
ACGAACCTTCAGAAGATTCA+TGG 0.454895 8.3:-32574067 MS.gene056472:CDS
GGGGTGGGGATAGTGGTTGT+TGG 0.455018 8.3:+32574883 None:intergenic
TTAATCAGAACTCCGTTGTT+GGG 0.460189 8.3:-32572846 MS.gene056472:CDS
TTATGCATTGGCAAAGAAGC+TGG 0.473516 8.3:-32572793 MS.gene056472:CDS
GGTGGTAATGTGGGAAGGGA+TGG 0.475750 8.3:+32574795 None:intergenic
GAATGCACAATCAAAATTGT+TGG 0.476039 8.3:+32574351 None:intergenic
TAGTTGATTGCGCTTTCTCA+GGG 0.481392 8.3:+32574303 None:intergenic
TTGCGCAAATGTCAGAAACT+TGG 0.482429 8.3:-32572767 MS.gene056472:CDS
TATGATGTTATTGGTTGTGG+TGG 0.483931 8.3:+32575035 None:intergenic
TCTAACATAGGTGTCATGTT+TGG 0.484082 8.3:+32572628 None:intergenic
GTTGGTTGCGATTTCACTGC+GGG 0.484377 8.3:+32574369 None:intergenic
CGGTTTAAGTGGGGTAATTG+GGG 0.485800 8.3:+32574948 None:intergenic
GTTGTTGTTCGATGAGTGTT+GGG 0.487764 8.3:+32574713 None:intergenic
GGTAGGAGTAGTGGTTGGGG+TGG 0.493221 8.3:+32574831 None:intergenic
GTTAATCAGAACTCCGTTGT+TGG 0.498948 8.3:-32572847 MS.gene056472:CDS
GTGGTGGTTAGAGTTGGAGG+TGG 0.500554 8.3:+32575051 None:intergenic
ATAGAGCACCACTATTGATC+TGG 0.502409 8.3:+32572584 None:intergenic
GTTGTTGTGGAATTGATGAA+TGG 0.506516 8.3:+32574326 None:intergenic
TTGCTCTCTGGCTGATCTAA+CGG 0.509856 8.3:+32572721 None:intergenic
ACGGAGTTCTGATTAACTGC+TGG 0.513492 8.3:+32572853 None:intergenic
TGAAATCATTCGGTTTAAGT+GGG 0.513951 8.3:+32574938 None:intergenic
TGTTGTTGTTCGATGAGTGT+TGG 0.514177 8.3:+32574712 None:intergenic
GGAGTTTCAAAATTGGAGGT+AGG 0.515498 8.3:+32575072 None:intergenic
TGATGGTATTGGTGGTAATG+TGG 0.520234 8.3:+32574785 None:intergenic
AGGTGGAGTTTCAAAATTGG+AGG 0.521183 8.3:+32575068 None:intergenic
CATGCCCATGAATCTTCTGA+AGG 0.531817 8.3:+32574062 None:intergenic
TGTTGGGAAATTAGAAAAGG+TGG 0.536200 8.3:+32574729 None:intergenic
ATCAAGAGATCCTCTAACAT+AGG 0.542974 8.3:+32572616 None:intergenic
GCGATTTCACTGCGGGCATG+GGG 0.547693 8.3:+32574376 None:intergenic
GATGGTATTGGTGGTAATGT+GGG 0.548603 8.3:+32574786 None:intergenic
TCCCAATCATCATTGTCCAT+TGG 0.550956 8.3:+32575141 None:intergenic
ATACTAGTGATAGTAGGGGT+GGG 0.555078 8.3:+32574868 None:intergenic
GGAGGTAGGAGTAGCAAAGG+TGG 0.555353 8.3:+32575086 None:intergenic
TACTAGTGATAGTAGGGGTG+GGG 0.555369 8.3:+32574869 None:intergenic
CTTCAGAAGATTCATGGGCA+TGG 0.559542 8.3:-32574061 MS.gene056472:CDS
GACTTGAACCAGATCAATAG+TGG 0.561347 8.3:-32572592 MS.gene056472:CDS
GAGTGTTGGGAAATTAGAAA+AGG 0.562639 8.3:+32574726 None:intergenic
ACTAGTGTTAGTGATGGTAT+TGG 0.562993 8.3:+32574774 None:intergenic
CATGGCACACAAATGTCACT+TGG 0.574104 8.3:+32574099 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.584498 8.3:-32574016 MS.gene056472:intron
CGAACCTTCAGAAGATTCAT+GGG 0.585253 8.3:-32574066 MS.gene056472:CDS
AAACTAAAATCATCAAAGCA+TGG 0.588328 8.3:+32574991 None:intergenic
TCAAAGACTAGATTATGCAT+TGG 0.588584 8.3:-32572805 MS.gene056472:CDS
AAGGTTCGTCTTCCCTCACA+TGG 0.591333 8.3:+32574081 None:intergenic
ATCATCAAAGCATGGAGGTG+TGG 0.594915 8.3:+32574999 None:intergenic
ATGAAAACTAGTGTTAGTGA+TGG 0.596957 8.3:+32574768 None:intergenic
AGTGTTAGTGATGGTATTGG+TGG 0.606164 8.3:+32574777 None:intergenic
GTATTGGTGGTAATGTGGGA+AGG 0.610190 8.3:+32574790 None:intergenic
GATACTAGTGATAGTAGGGG+TGG 0.616026 8.3:+32574867 None:intergenic
TATTGGTGGTAATGTGGGAA+GGG 0.622832 8.3:+32574791 None:intergenic
GACATTTGTGTGCCATGTGA+GGG 0.624856 8.3:-32574093 MS.gene056472:CDS
GATGTGGAGACCCTTTAATG+GGG 0.631825 8.3:+32574021 None:intergenic
ATTGGAGGTAGGAGTAGCAA+AGG 0.639288 8.3:+32575083 None:intergenic
GAAATCATTCGGTTTAAGTG+GGG 0.641149 8.3:+32574939 None:intergenic
TGACATTTGTGTGCCATGTG+AGG 0.643817 8.3:-32574094 MS.gene056472:CDS
AGTTGATTGCGCTTTCTCAG+GGG 0.662828 8.3:+32574304 None:intergenic
TGTTGGTTGCGATTTCACTG+CGG 0.712957 8.3:+32574368 None:intergenic
TGGGATACTAGTGATAGTAG+GGG 0.723289 8.3:+32574864 None:intergenic
CTAAAATCATCAAAGCATGG+AGG 0.732238 8.3:+32574994 None:intergenic
AGATCAGCCAGAGAGCAACG+AGG 0.772309 8.3:-32572716 MS.gene056472:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATATAATAAATAAT+AGG + chr8.3:32573531-32573550 None:intergenic 0.0%
!! AAATTTATTTATAGTGAAAT+TGG - chr8.3:32574234-32574253 MS.gene056472:CDS 10.0%
!!! TTCACTATAAATAAATTTTA+CGG + chr8.3:32574232-32574251 None:intergenic 10.0%
!! CAAAATTTAAATCAAGAAAA+AGG - chr8.3:32573492-32573511 MS.gene056472:intron 15.0%
!! TATCAAAATAAGGATTAAAA+TGG + chr8.3:32574272-32574291 None:intergenic 15.0%
!!! ATGAAATGTATAATGATTTT+AGG + chr8.3:32573573-32573592 None:intergenic 15.0%
!!! CTTTTTCTTGATTTAAATTT+TGG + chr8.3:32573494-32573513 None:intergenic 15.0%
!!! GATTTTATTCTATCAAAATA+AGG + chr8.3:32574282-32574301 None:intergenic 15.0%
!! AAAAAAATGATACATTTGCA+AGG + chr8.3:32574134-32574153 None:intergenic 20.0%
!! ATCAAAATAGTATGATTTAG+CGG - chr8.3:32574685-32574704 MS.gene056472:CDS 20.0%
!! TAAATAAAGTGAAAGATCTT+AGG + chr8.3:32574522-32574541 None:intergenic 20.0%
!! TGTATACATTTATATGTGTA+TGG - chr8.3:32574654-32574673 MS.gene056472:intron 20.0%
!!! AAATATACCACTTTTTCTTT+TGG + chr8.3:32574378-32574397 None:intergenic 20.0%
!!! AATGTATGTTAATTGCTTTT+TGG - chr8.3:32574091-32574110 MS.gene056472:CDS 20.0%
!!! ATATCAATGAATGTTTTTGT+AGG - chr8.3:32574724-32574743 MS.gene056472:CDS 20.0%
! AAAAGAGAGGATATATTTGT+AGG - chr8.3:32574422-32574441 MS.gene056472:intron 25.0%
! AAACATACACTTCATTGTTA+CGG + chr8.3:32573313-32573332 None:intergenic 25.0%
! AAACTAAAATCATCAAAGCA+TGG + chr8.3:32572726-32572745 None:intergenic 25.0%
! AAAGAGAGGATATATTTGTA+GGG - chr8.3:32574423-32574442 MS.gene056472:intron 25.0%
! AGGATTAAAATGGATATTGA+AGG + chr8.3:32574262-32574281 None:intergenic 25.0%
! ATAAAGACCAAAAGAAAAAG+TGG - chr8.3:32574368-32574387 MS.gene056472:CDS 25.0%
! CTAATATGAATAAAGTTTGC+AGG - chr8.3:32574331-32574350 MS.gene056472:CDS 25.0%
! GGATTAAAATGGATATTGAA+GGG + chr8.3:32574261-32574280 None:intergenic 25.0%
! TTAACTACAATTCTTTCCAA+TGG + chr8.3:32573931-32573950 None:intergenic 25.0%
!! AAAAATCTAGAATTTGTTCG+AGG + chr8.3:32573449-32573468 None:intergenic 25.0%
!! CACTATTTAAAACTCTAGTT+GGG + chr8.3:32573886-32573905 None:intergenic 25.0%
!! TCACTATTTAAAACTCTAGT+TGG + chr8.3:32573887-32573906 None:intergenic 25.0%
AAGGAAGAAAAAGAAAAGAG+AGG - chr8.3:32574409-32574428 MS.gene056472:CDS 30.0%
AATTGAAATGCAAATGCAAC+CGG + chr8.3:32573977-32573996 None:intergenic 30.0%
ACAATTACACCTTTGATCTT+TGG - chr8.3:32574184-32574203 MS.gene056472:intron 30.0%
ACTACAAATGTAGAAGTTTC+TGG - chr8.3:32574751-32574770 MS.gene056472:CDS 30.0%
ACTTATGTCGTGACATATAA+AGG - chr8.3:32574822-32574841 MS.gene056472:CDS 30.0%
ATGAAAACTAGTGTTAGTGA+TGG + chr8.3:32572949-32572968 None:intergenic 30.0%
ATGAAATCATTCGGTTTAAG+TGG + chr8.3:32572780-32572799 None:intergenic 30.0%
CGTTTAATGCATAAAAACCT+TGG + chr8.3:32573718-32573737 None:intergenic 30.0%
CTACAAATGTAGAAGTTTCT+GGG - chr8.3:32574752-32574771 MS.gene056472:CDS 30.0%
GAATGCACAATCAAAATTGT+TGG + chr8.3:32573366-32573385 None:intergenic 30.0%
GTTTACTGAGCTAGAAAAAA+TGG - chr8.3:32573040-32573059 MS.gene056472:intron 30.0%
TACAATTCTTTCCAATGGTA+AGG + chr8.3:32573926-32573945 None:intergenic 30.0%
TCAAAGACTAGATTATGCAT+TGG - chr8.3:32574909-32574928 MS.gene056472:CDS 30.0%
TCAAGCTCTATGAAATCATT+CGG + chr8.3:32572789-32572808 None:intergenic 30.0%
TGAAATCATTCGGTTTAAGT+GGG + chr8.3:32572779-32572798 None:intergenic 30.0%
TGTAACAAAATTCTTTCCGA+TGG + chr8.3:32573852-32573871 None:intergenic 30.0%
TGTGTTTCCTAAAAAACAGA+GGG + chr8.3:32574167-32574186 None:intergenic 30.0%
TTGTGTTTCCTAAAAAACAG+AGG + chr8.3:32574168-32574187 None:intergenic 30.0%
! CACTATTTAAAACTCTAGGT+GGG + chr8.3:32573807-32573826 None:intergenic 30.0%
! CATGAGAGAGTTTTACATAA+AGG + chr8.3:32574636-32574655 None:intergenic 30.0%
! TTACATAAAGGTATGCAATC+TGG + chr8.3:32574624-32574643 None:intergenic 30.0%
!! AGAAAAGGTGGAATTTTGAT+TGG + chr8.3:32572976-32572995 None:intergenic 30.0%
!!! AGTCTTTGATGACTTGTTTT+GGG + chr8.3:32574898-32574917 None:intergenic 30.0%
!!! AGTTTTAAATAGTGATCGCA+AGG - chr8.3:32573892-32573911 MS.gene056472:intron 30.0%
!!! GGTTTTTTCGAATGTGTATT+TGG + chr8.3:32573473-32573492 None:intergenic 30.0%
!!! TAGTCTTTGATGACTTGTTT+TGG + chr8.3:32574899-32574918 None:intergenic 30.0%
AAACATGACACCTATGTTAG+AGG - chr8.3:32575088-32575107 MS.gene056472:CDS 35.0%
AAACTTGGAATCGCCTAATA+TGG - chr8.3:32574962-32574981 MS.gene056472:CDS 35.0%
ACTAGTATTAGTCGTTGTAG+TGG + chr8.3:32573216-32573235 None:intergenic 35.0%
ACTAGTGTTAGTGATGGTAT+TGG + chr8.3:32572943-32572962 None:intergenic 35.0%
ATCAAGAGATCCTCTAACAT+AGG + chr8.3:32575101-32575120 None:intergenic 35.0%
ATGCATAAAAACCTTGGATG+TGG + chr8.3:32573712-32573731 None:intergenic 35.0%
ATGGAGAAAACCCCATTAAA+GGG - chr8.3:32573683-32573702 MS.gene056472:intron 35.0%
ATTGAAATGCAAATGCAACC+GGG + chr8.3:32573976-32573995 None:intergenic 35.0%
CAAAAACTCCATAGACGTAT+CGG + chr8.3:32575036-32575055 None:intergenic 35.0%
CAAAATTCTTTCCGATGGTA+AGG + chr8.3:32573847-32573866 None:intergenic 35.0%
CATCAAAGACCAAAGATCAA+AGG + chr8.3:32574196-32574215 None:intergenic 35.0%
CTAAAATCATCAAAGCATGG+AGG + chr8.3:32572723-32572742 None:intergenic 35.0%
CTATGTTACTCAGTGTAAGA+CGG - chr8.3:32574596-32574615 MS.gene056472:intron 35.0%
GAAATCATTCGGTTTAAGTG+GGG + chr8.3:32572778-32572797 None:intergenic 35.0%
GTAGTTAAATGCAACATACG+TGG - chr8.3:32573943-32573962 MS.gene056472:intron 35.0%
GTTGTTGTGGAATTGATGAA+TGG + chr8.3:32573391-32573410 None:intergenic 35.0%
TACACTTCATTGTTACGGTT+TGG + chr8.3:32573308-32573327 None:intergenic 35.0%
TATGGAGAAAACCCCATTAA+AGG - chr8.3:32573682-32573701 MS.gene056472:intron 35.0%
TATGTTAGAGGATCTCTTGA+TGG - chr8.3:32575100-32575119 MS.gene056472:CDS 35.0%
TCATGGGCATGGAAAAAATA+TGG - chr8.3:32573664-32573683 MS.gene056472:intron 35.0%
TCTTACACTGAGTAACATAG+TGG + chr8.3:32574596-32574615 None:intergenic 35.0%
TGTTGGGAAATTAGAAAAGG+TGG + chr8.3:32572988-32573007 None:intergenic 35.0%
TTAATCAGAACTCCGTTGTT+GGG - chr8.3:32574868-32574887 MS.gene056472:CDS 35.0%
TTGAGGGGATATGATGTTAT+TGG + chr8.3:32572691-32572710 None:intergenic 35.0%
TTGGGATACTAGTGATAGTA+GGG + chr8.3:32572854-32572873 None:intergenic 35.0%
TTTCCTAAAAAACAGAGGGA+CGG + chr8.3:32574163-32574182 None:intergenic 35.0%
TTTGTTCGAGGTAGTAGAAA+TGG + chr8.3:32573437-32573456 None:intergenic 35.0%
! CCACTATTTAAAACTCTAGG+TGG + chr8.3:32573808-32573827 None:intergenic 35.0%
! CGACCACTATTTAAAACTCT+AGG + chr8.3:32573811-32573830 None:intergenic 35.0%
! GATACGTCTATGGAGTTTTT+GGG - chr8.3:32575035-32575054 MS.gene056472:CDS 35.0%
! GGATATGATGTTATTGGTTG+TGG + chr8.3:32572685-32572704 None:intergenic 35.0%
! TATGATGTTATTGGTTGTGG+TGG + chr8.3:32572682-32572701 None:intergenic 35.0%
! TCTAACATAGGTGTCATGTT+TGG + chr8.3:32575089-32575108 None:intergenic 35.0%
!! GAGTGTTGGGAAATTAGAAA+AGG + chr8.3:32572991-32573010 None:intergenic 35.0%
!! TAGAAGTTTCTGGGATTGTT+CGG - chr8.3:32574761-32574780 MS.gene056472:CDS 35.0%
!!! CCACCTAGAGTTTTAAATAG+TGG - chr8.3:32573805-32573824 MS.gene056472:intron 35.0%
AACGGCAACATAGCCATATT+AGG + chr8.3:32574978-32574997 None:intergenic 40.0%
AAGACCATGATCCTTACCAT+CGG - chr8.3:32573833-32573852 MS.gene056472:intron 40.0%
ACGAACCTTCAGAAGATTCA+TGG - chr8.3:32573647-32573666 MS.gene056472:intron 40.0%
AGGTGGAGTTTCAAAATTGG+AGG + chr8.3:32572649-32572668 None:intergenic 40.0%
ATACTAGTGATAGTAGGGGT+GGG + chr8.3:32572849-32572868 None:intergenic 40.0%
ATTAGTCGTTGTAGTGGTGA+TGG + chr8.3:32573210-32573229 None:intergenic 40.0%
CGAACCTTCAGAAGATTCAT+GGG - chr8.3:32573648-32573667 MS.gene056472:intron 40.0%
CTTTCCAATGGTAAGGATCA+TGG + chr8.3:32573919-32573938 None:intergenic 40.0%
GACTTGAACCAGATCAATAG+TGG - chr8.3:32575122-32575141 MS.gene056472:CDS 40.0%
GGAGTTTCAAAATTGGAGGT+AGG + chr8.3:32572645-32572664 None:intergenic 40.0%
GTTAATCAGAACTCCGTTGT+TGG - chr8.3:32574867-32574886 MS.gene056472:CDS 40.0%
GTTGGGATACTAGTGATAGT+AGG + chr8.3:32572855-32572874 None:intergenic 40.0%
TAGTTGATTGCGCTTTCTCA+GGG + chr8.3:32573414-32573433 None:intergenic 40.0%
TCGGTTTAAGTGGGGTAATT+GGG + chr8.3:32572770-32572789 None:intergenic 40.0%
TGGAGGTGGAGTTTCAAAAT+TGG + chr8.3:32572652-32572671 None:intergenic 40.0%
TGGGATACTAGTGATAGTAG+GGG + chr8.3:32572853-32572872 None:intergenic 40.0%
TTCGGTTTAAGTGGGGTAAT+TGG + chr8.3:32572771-32572790 None:intergenic 40.0%
TTGCGCAAATGTCAGAAACT+TGG - chr8.3:32574947-32574966 MS.gene056472:CDS 40.0%
! AACATACGTGGTCTTAATCC+CGG - chr8.3:32573955-32573974 MS.gene056472:intron 40.0%
! AGTGTTAGTGATGGTATTGG+TGG + chr8.3:32572940-32572959 None:intergenic 40.0%
! ATAGAGCACCACTATTGATC+TGG + chr8.3:32575133-32575152 None:intergenic 40.0%
! CGATACGTCTATGGAGTTTT+TGG - chr8.3:32575034-32575053 MS.gene056472:CDS 40.0%
! GTAGTGAAGATGGTGGTAAT+GGG + chr8.3:32573122-32573141 None:intergenic 40.0%
! GTTGTTGTTCGATGAGTGTT+GGG + chr8.3:32573004-32573023 None:intergenic 40.0%
! TAGTGAAGATGGTGGTAATG+GGG + chr8.3:32573121-32573140 None:intergenic 40.0%
! TATTGGTGGTAATGTGGGAA+GGG + chr8.3:32572926-32572945 None:intergenic 40.0%
! TGGGGCTGATTTATTCTCTT+TGG + chr8.3:32572751-32572770 None:intergenic 40.0%
! TGTTGTTGTTCGATGAGTGT+TGG + chr8.3:32573005-32573024 None:intergenic 40.0%
! TTATGCATTGGCAAAGAAGC+TGG - chr8.3:32574921-32574940 MS.gene056472:CDS 40.0%
! TTGTAGGGATAGTGGTTGTA+GGG + chr8.3:32573145-32573164 None:intergenic 40.0%
!! GATGGTATTGGTGGTAATGT+GGG + chr8.3:32572931-32572950 None:intergenic 40.0%
!! TGATGGTATTGGTGGTAATG+TGG + chr8.3:32572932-32572951 None:intergenic 40.0%
!! TGGTGATGGTAGGTATGATT+GGG + chr8.3:32573196-32573215 None:intergenic 40.0%
!!! CTTTGATGACTTGTTTTGGG+AGG + chr8.3:32574895-32574914 None:intergenic 40.0%
AGGACCATGATCCTTACCAT+TGG - chr8.3:32573912-32573931 MS.gene056472:intron 45.0%
AGTTCTCACCGATACGTCTA+TGG - chr8.3:32575025-32575044 MS.gene056472:CDS 45.0%
AGTTGATTGCGCTTTCTCAG+GGG + chr8.3:32573413-32573432 None:intergenic 45.0%
ATCATCAAAGCATGGAGGTG+TGG + chr8.3:32572718-32572737 None:intergenic 45.0%
ATTGGAGGTAGGAGTAGCAA+AGG + chr8.3:32572634-32572653 None:intergenic 45.0%
CATGCCCATGAATCTTCTGA+AGG + chr8.3:32573655-32573674 None:intergenic 45.0%
CATGGCACACAAATGTCACT+TGG + chr8.3:32573618-32573637 None:intergenic 45.0%
CGGTTTAAGTGGGGTAATTG+GGG + chr8.3:32572769-32572788 None:intergenic 45.0%
CTTCAGAAGATTCATGGGCA+TGG - chr8.3:32573653-32573672 MS.gene056472:intron 45.0%
CTTTCCGATGGTAAGGATCA+TGG + chr8.3:32573840-32573859 None:intergenic 45.0%
GAATGTGGTTGTAGGGATAG+TGG + chr8.3:32573153-32573172 None:intergenic 45.0%
GACATTTGTGTGCCATGTGA+GGG - chr8.3:32573621-32573640 MS.gene056472:intron 45.0%
GATACTAGTGATAGTAGGGG+TGG + chr8.3:32572850-32572869 None:intergenic 45.0%
GATGTGGAGACCCTTTAATG+GGG + chr8.3:32573696-32573715 None:intergenic 45.0%
GGATGTGGAGACCCTTTAAT+GGG + chr8.3:32573697-32573716 None:intergenic 45.0%
GTAGTTGATTGCGCTTTCTC+AGG + chr8.3:32573415-32573434 None:intergenic 45.0%
TACTAGTGATAGTAGGGGTG+GGG + chr8.3:32572848-32572867 None:intergenic 45.0%
TGACATTTGTGTGCCATGTG+AGG - chr8.3:32573620-32573639 MS.gene056472:intron 45.0%
TGGATGTGGAGACCCTTTAA+TGG + chr8.3:32573698-32573717 None:intergenic 45.0%
TTAAAGGGTCTCCACATCCA+AGG - chr8.3:32573698-32573717 MS.gene056472:intron 45.0%
TTGCTCTCTGGCTGATCTAA+CGG + chr8.3:32574996-32575015 None:intergenic 45.0%
TTGGTTGTGGTGGTTAGAGT+TGG + chr8.3:32572672-32572691 None:intergenic 45.0%
! ACGGAGTTCTGATTAACTGC+TGG + chr8.3:32574864-32574883 None:intergenic 45.0%
! GGTAGTGAAGATGGTGGTAA+TGG + chr8.3:32573123-32573142 None:intergenic 45.0%
! GGTGATGGTAGGTATGATTG+GGG + chr8.3:32573195-32573214 None:intergenic 45.0%
! GTATTGGTGGTAATGTGGGA+AGG + chr8.3:32572927-32572946 None:intergenic 45.0%
! GTGTCGCTCATTGCAAAGAA+TGG + chr8.3:32574022-32574041 None:intergenic 45.0%
! GTTGTAGGGATAGTGGTTGT+AGG + chr8.3:32573146-32573165 None:intergenic 45.0%
! TGTAGGGATAGTGGTTGTAG+GGG + chr8.3:32573144-32573163 None:intergenic 45.0%
! TGTTGGTTGCGATTTCACTG+CGG + chr8.3:32573349-32573368 None:intergenic 45.0%
!! AGTGAAGATGGTGGTAATGG+GGG + chr8.3:32573120-32573139 None:intergenic 45.0%
!! ATGGAGGTGTGGTGTTTTGA+GGG + chr8.3:32572707-32572726 None:intergenic 45.0%
!! GTGGTGATGGTAGGTATGAT+TGG + chr8.3:32573197-32573216 None:intergenic 45.0%
!!! GTTCCGTCCCTCTGTTTTTT+AGG - chr8.3:32574157-32574176 MS.gene056472:intron 45.0%
!!! GTATATTTTTTTAAAAAAAA+AGG - chr8.3:32574390-32574409 MS.gene056472:CDS 5.0%
AAGGTTCGTCTTCCCTCACA+TGG + chr8.3:32573636-32573655 None:intergenic 50.0%
AGGTATGATTGGGGTTGTGG+TGG + chr8.3:32573186-32573205 None:intergenic 50.0%
CTCTAGGTGGGTGTGTGTTT+AGG + chr8.3:32573795-32573814 None:intergenic 50.0%
GGTAGGTATGATTGGGGTTG+TGG + chr8.3:32573189-32573208 None:intergenic 50.0%
GGTTGTAGGGGTAGTGAAGA+TGG + chr8.3:32573132-32573151 None:intergenic 50.0%
GTTGGTTGCGATTTCACTGC+GGG + chr8.3:32573348-32573367 None:intergenic 50.0%
GTTGTGGTGGTTAGAGTTGG+AGG + chr8.3:32572669-32572688 None:intergenic 50.0%
TGAGCAGTGATGGTAGAGGT+GGG + chr8.3:32573095-32573114 None:intergenic 50.0%
TGTAGGGGTAGTGAAGATGG+TGG + chr8.3:32573129-32573148 None:intergenic 50.0%
! TTGGGGTGGTAGTGATGGTT+GGG + chr8.3:32572872-32572891 None:intergenic 50.0%
!! CATGGAGGTGTGGTGTTTTG+AGG + chr8.3:32572708-32572727 None:intergenic 50.0%
!! GTCGTTGTAGTGGTGATGGT+AGG + chr8.3:32573206-32573225 None:intergenic 50.0%
!! TGGAGGTGTGGTGTTTTGAG+GGG + chr8.3:32572706-32572725 None:intergenic 50.0%
!!! GTTTTGGGAGGTCCCAACAA+CGG + chr8.3:32574883-32574902 None:intergenic 50.0%
ACTTGAGCCTCGTTGCTCTC+TGG + chr8.3:32575008-32575027 None:intergenic 55.0%
AGATCAGCCAGAGAGCAACG+AGG - chr8.3:32574998-32575017 MS.gene056472:CDS 55.0%
AGGGGGTAGGAGTAGTGGTT+GGG + chr8.3:32572890-32572909 None:intergenic 55.0%
AGGGTGGCGAATGTGGTTGT+AGG + chr8.3:32573161-32573180 None:intergenic 55.0%
CGCTTTCTCAGGGGTTGTTG+TGG + chr8.3:32573404-32573423 None:intergenic 55.0%
GAGCAGTGATGGTAGAGGTG+GGG + chr8.3:32573094-32573113 None:intergenic 55.0%
GATAGTAGGGGTGGGGATAG+TGG + chr8.3:32572841-32572860 None:intergenic 55.0%
GATGGTAGAGGTGGGGATAG+TGG + chr8.3:32573087-32573106 None:intergenic 55.0%
GGAGGTAGGAGTAGCAAAGG+TGG + chr8.3:32572631-32572650 None:intergenic 55.0%
GGGTGGCGAATGTGGTTGTA+GGG + chr8.3:32573160-32573179 None:intergenic 55.0%
GGTAATGTGGGAAGGGATGG+TGG + chr8.3:32572919-32572938 None:intergenic 55.0%
GGTGTGAGCAGTGATGGTAG+AGG + chr8.3:32573099-32573118 None:intergenic 55.0%
GTGAGCAGTGATGGTAGAGG+TGG + chr8.3:32573096-32573115 None:intergenic 55.0%
GTGGTGGTTAGAGTTGGAGG+TGG + chr8.3:32572666-32572685 None:intergenic 55.0%
TGCGATTTCACTGCGGGCAT+GGG + chr8.3:32573342-32573361 None:intergenic 55.0%
TTGCGATTTCACTGCGGGCA+TGG + chr8.3:32573343-32573362 None:intergenic 55.0%
! AAGGGATGGTGGTGCTTGAG+GGG + chr8.3:32572908-32572927 None:intergenic 55.0%
! AATGGGGGTGTGAGCAGTGA+TGG + chr8.3:32573105-32573124 None:intergenic 55.0%
! AGAGGTGGGGATAGTGGTGT+TGG + chr8.3:32573081-32573100 None:intergenic 55.0%
! AGGTGGGGATAGTGGTGTTG+GGG + chr8.3:32573079-32573098 None:intergenic 55.0%
! AGTGGTTGGGGTGGTAGTGA+TGG + chr8.3:32572877-32572896 None:intergenic 55.0%
! GAAGGGATGGTGGTGCTTGA+GGG + chr8.3:32572909-32572928 None:intergenic 55.0%
! GAGGTGGGGATAGTGGTGTT+GGG + chr8.3:32573080-32573099 None:intergenic 55.0%
! GATTGGGGTTGTGGTGGTGA+GGG + chr8.3:32573180-32573199 None:intergenic 55.0%
! GGTGGTAATGTGGGAAGGGA+TGG + chr8.3:32572922-32572941 None:intergenic 55.0%
! GTTGGGGTGGTAGTGATGGT+TGG + chr8.3:32572873-32572892 None:intergenic 55.0%
! TGATTGGGGTTGTGGTGGTG+AGG + chr8.3:32573181-32573200 None:intergenic 55.0%
GAGGGGGTAGGAGTAGTGGT+TGG + chr8.3:32572891-32572910 None:intergenic 60.0%
GCGATTTCACTGCGGGCATG+GGG + chr8.3:32573341-32573360 None:intergenic 60.0%
GCTTGAGGGGGTAGGAGTAG+TGG + chr8.3:32572895-32572914 None:intergenic 60.0%
GGGGGTAGGAGTAGTGGTTG+GGG + chr8.3:32572889-32572908 None:intergenic 60.0%
GGTAGGAGTAGTGGTTGGGG+TGG + chr8.3:32572886-32572905 None:intergenic 60.0%
! AGGGATGGTGGTGCTTGAGG+GGG + chr8.3:32572907-32572926 None:intergenic 60.0%
! ATGGTGGTGCTTGAGGGGGT+AGG + chr8.3:32572903-32572922 None:intergenic 60.0%
! GGAAGGGATGGTGGTGCTTG+AGG + chr8.3:32572910-32572929 None:intergenic 60.0%
! GGGGTGGGGATAGTGGTTGT+TGG + chr8.3:32572834-32572853 None:intergenic 60.0%
GGTGGTGAGGGTGGCGAATG+TGG + chr8.3:32573168-32573187 None:intergenic 65.0%
! TGGGGTTGTGGTGGTGAGGG+TGG + chr8.3:32573177-32573196 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 32572576 32575160 32572576 ID=MS.gene056472
chr8.3 mRNA 32572576 32575160 32572576 ID=MS.gene056472.t1;Parent=MS.gene056472
chr8.3 exon 32574675 32575160 32574675 ID=MS.gene056472.t1.exon1;Parent=MS.gene056472.t1
chr8.3 CDS 32574675 32575160 32574675 ID=cds.MS.gene056472.t1;Parent=MS.gene056472.t1
chr8.3 exon 32574223 32574437 32574223 ID=MS.gene056472.t1.exon2;Parent=MS.gene056472.t1
chr8.3 CDS 32574223 32574437 32574223 ID=cds.MS.gene056472.t1;Parent=MS.gene056472.t1
chr8.3 exon 32574017 32574133 32574017 ID=MS.gene056472.t1.exon3;Parent=MS.gene056472.t1
chr8.3 CDS 32574017 32574133 32574017 ID=cds.MS.gene056472.t1;Parent=MS.gene056472.t1
chr8.3 exon 32572576 32572990 32572576 ID=MS.gene056472.t1.exon4;Parent=MS.gene056472.t1
chr8.3 CDS 32572576 32572990 32572576 ID=cds.MS.gene056472.t1;Parent=MS.gene056472.t1
Gene Sequence

>MS.gene056472

ATGGACAATGATGATTGGGATCTCTTTTCTATTGTTCGTAGCTGCAAAGCTACCACCTTTGCTACTCCTACCTCCAATTTTGAAACTCCACCTCCAACTCTAACCACCACAACCAATAACATCATATCCCCTCAAAACACCACACCTCCATGCTTTGATGATTTTAGTTTTACCAAAGAGAATAAATCAGCCCCAATTACCCCACTTAAACCGAATGATTTCATAGAGCTTGACAAATTGATAATCAGTTTCAATCCAACAACCACTATCCCCACCCCTACTATCACTAGTATCCCAACCATCACTACCACCCCAACCACTACTCCTACCCCCTCAAGCACCACCATCCCTTCCCACATTACCACCAATACCATCACTAACACTAGTTTTCATGATTCCAATCAAAATTCCACCTTTTCTAATTTCCCAACACTCATCGAACAACAACAACTGCAACTAAATGAGTTTACTGAGCTAGAAAAAATGGTACTTAAATTCTACCCCAACACCACTATCCCCACCTCTACCATCACTGCTCACACCCCCATTACCACCATCTTCACTACCCCTACAACCACTATCCCTACAACCACATTCGCCACCCTCACCACCACAACCCCAATCATACCTACCATCACCACTACAACGACTAATACTAGTGTTTGTAAAACAAATCAATATCTTACAATTTCTGACTATCAAATACTCGTCGAACAACAACAGAATCAACCAAACCGTAACAATGAAGTGTATGTTTCTGAACCCCATGCCCGCAGTGAAATCGCAACCAACAATTTTGATTGTGCATTCAACCATTCATCAATTCCACAACAACCCCTGAGAAAGCGCAATCAACTACCATTTCTACTACCTCGAACAAATTCTAGATTTTTACCAAATACACATTCGAAAAAACCAAAATTTAAATCAAGAAAAAGGTAAAGTATTTATTCCTATTATTTATTATATTTTAATTATATCAATTTTATTGTGACCTAAAATCATTATACATTTCATTTTTTTACAGAAAAAATAATAACCAAGTGACATTTGTGTGCCATGTGAGGGAAGACGAACCTTCAGAAGATTCATGGGCATGGAAAAAATATGGAGAAAACCCCATTAAAGGGTCTCCACATCCAAGGTTTTTATGCATTAAACGCTCTAAATTTACATATTTGTCTTCTATTTCATGTTAAAAATAATAATTTTGTTACCTAAACACACACCCACCTAGAGTTTTAAATAGTGGTCGCAAAGACCATGATCCTTACCATCGGAAAGAATTTTGTTACATAATACACACACCCAACTAGAGTTTTAAATAGTGATCGCAAGGACCATGATCCTTACCATTGGAAAGAATTGTAGTTAAATGCAACATACGTGGTCTTAATCCCGGTTGCATTTGCATTTCAATTAAAAATATCAAAAGTTATTACTCCATTCTTTGCAATGAGCGACACTTTTTCAAAATTGAATTTTGTGACAAAATGAATGTCACTATTAATTTTCAATGTATGTTAATTGCTTTTTGGTTTGATATGTTCTTAGTCCTTGCAAATGTATCATTTTTTTTGCGTTCCGTCCCTCTGTTTTTTAGGAAACACAATTACACCTTTGATCTTTGGTCTTTGATGTTTGTTTTAGTTTCCGTAAAATTTATTTATAGTGAAATTGGTCCCTTCAATATCCATTTTAATCCTTATTTTGATAGAATAAAATCAAATATTCTACATATTAGAGAGATCAATTCTAATATGAATAAAGTTTGCAGGAGTAAAACAAAACAATAAAGACCAAAAGAAAAAGTGGTATATTTTTTTAAAAAAAAAGGAAGAAAAAGAAAAGAGAGGATATATTTGTAGGGACTAAAAATATATTTAAATATCACTTTTTATTTTTTTCAATTGTAATATTTAATTGATTTTAAACACATCACTCCTAAGATCTTTCACTTTATTTAAATCTCACTTCATTTATATATATATTTTTTAAAATATGTATAAAAATTAAACCACTATGTTACTCAGTGTAAGACGGATCCAGATTGCATACCTTTATGTAAAACTCTCTCATGTATACATTTATATGTGTATGGTTTTATCTATCAAAATAGTATGATTTAGCGGTGTTTGATATGAAAAGATATCAATGAATGTTTTTGTAGGAGCTACTACAAATGTAGAAGTTTCTGGGATTGTTCGGCAAAAAAACTAGTAGAGAAAAGCAAAACATGTGAGAACACTTATGTCGTGACATATAAAGGAGAACACAATCATAAACCAGCAGTTAATCAGAACTCCGTTGTTGGGACCTCCCAAAACAAGTCATCAAAGACTAGATTATGCATTGGCAAAGAAGCTGGATCTTGCGCAAATGTCAGAAACTTGGAATCGCCTAATATGGCTATGTTGCCGTTAGATCAGCCAGAGAGCAACGAGGCTCAAGTTCTCACCGATACGTCTATGGAGTTTTTGGGAAGCTCTGATGACGATGTCATTTTGATACCAAACATGACACCTATGTTAGAGGATCTCTTGATGGACTTGAACCAGATCAATAGTGGTGCTCTATTCCCTTAG
Protein sequence

>MS.gene056472.t1

MDNDDWDLFSIVRSCKATTFATPTSNFETPPPTLTTTTNNIISPQNTTPPCFDDFSFTKENKSAPITPLKPNDFIELDKLIISFNPTTTIPTPTITSIPTITTTPTTTPTPSSTTIPSHITTNTITNTSFHDSNQNSTFSNFPTLIEQQQLQLNEFTELEKMNQPNRNNEVYVSEPHARSEIATNNFDCAFNHSSIPQQPLRKRNQLPFLLPRTNSRFLPNTHSKKPKFKSRKRKNNNQVTFVCHVREDEPSEDSWAWKKYGENPIKGSPHPRSYYKCRSFWDCSAKKLVEKSKTCENTYVVTYKGEHNHKPAVNQNSVVGTSQNKSSKTRLCIGKEAGSCANVRNLESPNMAMLPLDQPESNEAQVLTDTSMEFLGSSDDDVILIPNMTPMLEDLLMDLNQINSGALFP