AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056486

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056486.t1 MTR_8g070075 88.942 633 64 1 1 633 132 758 0.0 1177
MS.gene056486.t1 MTR_8g070070 77.090 646 136 6 1 637 127 769 0.0 993
MS.gene056486.t1 MTR_8g071030 69.579 641 166 5 1 637 134 749 0.0 909
MS.gene056486.t1 MTR_1g035420 66.944 481 155 3 157 636 181 658 0.0 685
MS.gene056486.t1 MTR_1g035400 59.831 473 187 3 157 628 98 568 0.0 590
MS.gene056486.t1 MTR_6g044690 52.182 550 229 12 90 625 35 564 0.0 547
MS.gene056486.t1 MTR_0376s0030 53.306 484 201 7 164 636 108 577 2.44e-178 518
MS.gene056486.t1 MTR_6g043960 48.851 522 218 11 113 625 1 482 4.11e-162 474
MS.gene056486.t1 MTR_2g054460 46.529 533 202 14 107 625 1 464 1.42e-143 426
MS.gene056486.t1 MTR_5g013420 41.253 463 249 8 165 623 615 1058 4.11e-111 359
MS.gene056486.t1 MTR_5g013420 41.253 463 249 8 165 623 615 1058 4.29e-111 359
MS.gene056486.t1 MTR_4g010830 41.991 462 246 8 165 623 507 949 7.65e-108 348
MS.gene056486.t1 MTR_7g088370 41.189 471 241 13 165 625 236 680 2.40e-106 337
MS.gene056486.t1 MTR_3g011440 40.000 475 255 13 165 624 174 633 2.68e-104 330
MS.gene056486.t1 MTR_8g042750 39.207 454 248 11 185 625 220 658 3.43e-98 315
MS.gene056486.t1 MTR_4g010550 38.961 462 230 11 165 623 21 433 6.36e-93 295
MS.gene056486.t1 MTR_7g084090 37.882 491 267 15 158 624 251 727 8.89e-92 300
MS.gene056486.t1 MTR_6g061270 38.750 480 263 16 165 624 196 664 9.32e-90 293
MS.gene056486.t1 MTR_6g061200 37.849 465 246 15 171 624 203 635 1.13e-83 276
MS.gene056486.t1 MTR_7g084090 40.541 370 198 13 158 514 251 611 8.87e-74 250
MS.gene056486.t1 MTR_7g445710 32.773 476 227 18 168 623 110 512 1.99e-56 200
MS.gene056486.t1 MTR_7g445530 32.563 476 228 18 168 623 110 512 4.04e-56 199
MS.gene056486.t1 MTR_1g048950 32.421 475 272 12 164 623 67 507 1.11e-53 193
MS.gene056486.t1 MTR_7g450730 31.303 476 214 16 162 623 98 474 1.60e-53 191
MS.gene056486.t1 MTR_7g052480 36.154 260 153 7 375 624 270 526 4.18e-43 163
MS.gene056486.t1 MTR_8g078505 25.519 337 192 11 321 623 204 515 2.67e-22 101
MS.gene056486.t1 MTR_7g445600 34.000 150 62 2 474 623 243 355 4.09e-21 95.9
MS.gene056486.t1 MTR_7g445600 41.860 129 61 5 168 296 106 220 6.28e-16 80.1
MS.gene056486.t1 MTR_6g044000 38.400 125 72 2 113 237 1 120 8.20e-20 87.4
MS.gene056486.t1 MTR_6g044000 69.811 53 16 0 573 625 117 169 9.03e-20 87.4
MS.gene056486.t1 MTR_1g069570 26.710 307 153 12 375 625 430 720 9.06e-20 94.4
MS.gene056486.t1 MTR_8g078490 31.217 189 110 4 440 623 1056 1229 2.49e-19 93.2
MS.gene056486.t1 MTR_4g132610 30.270 185 115 4 441 623 851 1023 2.43e-18 90.1
MS.gene056486.t1 MTR_5g018850 31.034 203 119 6 440 637 1310 1496 2.74e-18 90.1
MS.gene056486.t1 MTR_1g080340 26.059 307 156 12 375 625 269 560 5.12e-18 88.2
MS.gene056486.t1 MTR_5g098680 36.076 158 87 4 193 345 267 415 6.65e-17 85.1
MS.gene056486.t1 MTR_1g069570 26.996 263 121 11 375 582 430 676 1.49e-16 84.0
MS.gene056486.t1 MTR_8g102580 35.443 158 88 4 193 345 261 409 3.10e-16 82.8
MS.gene056486.t1 MTR_7g098390 25.402 311 158 12 371 625 384 676 3.05e-15 79.7
MS.gene056486.t1 MTR_3g114910 33.333 153 88 4 193 345 187 325 5.41e-13 71.6
MS.gene056486.t1 MTR_7g445810 36.686 169 85 7 162 324 114 266 1.08e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056486.t1 AT4G13460 62.900 469 167 5 170 636 186 649 0.0 624
MS.gene056486.t1 AT4G13460 62.900 469 167 5 170 636 186 649 0.0 624
MS.gene056486.t1 AT2G33290 58.921 482 188 5 156 636 178 650 0.0 610
MS.gene056486.t1 AT2G33290 58.921 482 188 5 156 636 178 650 0.0 610
MS.gene056486.t1 AT2G33290 58.921 482 188 5 156 636 178 650 0.0 610
MS.gene056486.t1 AT2G33290 58.921 482 188 5 156 636 178 650 0.0 610
MS.gene056486.t1 AT2G35160 39.738 458 232 10 170 623 310 727 1.74e-97 315
MS.gene056486.t1 AT2G35160 39.738 458 232 10 170 623 346 763 5.11e-97 315
MS.gene056486.t1 AT2G35160 39.738 458 232 10 170 623 346 763 5.11e-97 315
MS.gene056486.t1 AT2G35160 39.738 458 232 10 170 623 346 763 5.11e-97 315
MS.gene056486.t1 AT2G35160 39.738 458 232 10 170 623 346 763 5.11e-97 315
MS.gene056486.t1 AT2G22740 38.316 475 270 8 158 623 299 759 9.76e-97 314
MS.gene056486.t1 AT2G22740 38.316 475 270 8 158 623 299 759 9.76e-97 314
MS.gene056486.t1 AT5G04940 39.869 459 233 12 185 625 207 640 7.51e-96 309
MS.gene056486.t1 AT5G04940 39.869 459 233 12 185 625 207 640 7.51e-96 309
MS.gene056486.t1 AT1G73100 37.908 459 244 12 185 625 204 639 8.05e-94 303
MS.gene056486.t1 AT5G13960 37.174 460 259 15 185 624 11 460 5.93e-86 277
MS.gene056486.t1 AT5G13960 36.458 480 274 16 165 624 126 594 1.00e-85 281
MS.gene056486.t1 AT1G17770 33.478 460 266 15 185 625 223 661 1.61e-72 247
MS.gene056486.t1 AT2G24740 36.123 454 240 15 186 625 307 724 8.03e-70 241
MS.gene056486.t1 AT5G47150 43.827 162 82 4 184 345 172 324 8.11e-30 120
MS.gene056486.t1 AT5G47160 42.105 171 89 5 173 342 247 408 1.06e-26 113
MS.gene056486.t1 AT5G47160 42.105 171 89 5 173 342 247 408 1.06e-26 113
MS.gene056486.t1 AT5G47160 42.105 171 89 5 173 342 247 408 1.06e-26 113
MS.gene056486.t1 AT2G23740 26.648 364 189 15 324 628 1012 1356 4.07e-23 105
MS.gene056486.t1 AT2G23740 26.648 364 189 15 324 628 1019 1363 4.12e-23 105
MS.gene056486.t1 AT2G23740 26.648 364 189 15 324 628 1019 1363 4.12e-23 105
MS.gene056486.t1 AT2G23740 26.648 364 189 15 324 628 1019 1363 4.12e-23 105
MS.gene056486.t1 AT2G05900 37.640 178 103 6 230 407 26 195 1.12e-20 93.6
MS.gene056486.t1 AT2G23740 26.032 315 158 14 324 582 1019 1314 1.94e-17 87.4
MS.gene056486.t1 AT2G23740 26.032 315 158 14 324 582 1019 1314 1.94e-17 87.4
MS.gene056486.t1 AT1G57820 35.849 159 87 5 193 345 277 426 3.28e-16 82.8
MS.gene056486.t1 AT1G57820 35.849 159 87 5 193 345 277 426 3.28e-16 82.8
MS.gene056486.t1 AT3G03750 30.583 206 115 6 421 624 144 323 2.96e-15 78.2
MS.gene056486.t1 AT1G57820 35.256 156 89 5 193 345 277 423 3.01e-15 79.7
MS.gene056486.t1 AT1G66040 33.333 159 91 4 193 345 262 411 4.97e-14 75.9
MS.gene056486.t1 AT5G39550 34.591 159 89 5 193 345 262 411 6.07e-14 75.5
MS.gene056486.t1 AT1G66050 33.333 159 91 4 193 345 262 411 6.29e-14 75.5
MS.gene056486.t1 AT1G04050 24.595 309 155 10 375 625 409 697 7.76e-14 75.5
MS.gene056486.t1 AT1G04050 24.595 309 155 10 375 625 409 697 7.76e-14 75.5
MS.gene056486.t1 AT1G04050 24.595 309 155 10 375 625 305 593 9.00e-14 75.1
MS.gene056486.t1 AT4G08590 31.818 154 91 4 193 345 237 377 2.68e-11 66.6

Find 164 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGGAAGATTAAGAAAAT+TGG 0.178663 8.3:+32416072 MS.gene056486:CDS
ACTAAATCATAACCTTAATA+TGG 0.228635 8.3:+32415039 MS.gene056486:CDS
ACTCGTTAATATATCCAAAA+AGG 0.268007 8.3:+32416589 MS.gene056486:CDS
TGTTTCTGAAGTTGCGATTA+TGG 0.269601 8.3:+32415381 MS.gene056486:CDS
TGATTCTTGTTTATTTGAAA+TGG 0.272590 8.3:-32415104 None:intergenic
AAGCAAACCCTGAAGATTAT+AGG 0.291078 8.3:-32416334 None:intergenic
AATTATTGAGGAGAAGAAAT+TGG 0.298748 8.3:+32415288 MS.gene056486:CDS
AGAGTTTCCCTATAATCTTC+AGG 0.299770 8.3:+32416326 MS.gene056486:CDS
AGCTTGCCCGCCCACTCGTC+AGG 0.315436 8.3:-32416882 None:intergenic
AGTAGCTCTGGTTAAGAAAA+AGG 0.316965 8.3:+32415135 MS.gene056486:CDS
AGCCTTTATCTGCGAGTATA+CGG 0.326598 8.3:+32416518 MS.gene056486:CDS
ATGTCATTAAATAGCCTAAT+AGG 0.327262 8.3:-32416156 None:intergenic
AAGTGGTTATGAGGATGATA+TGG 0.333296 8.3:+32415726 MS.gene056486:CDS
ATCATATTCGCGGTGACATA+TGG 0.333360 8.3:+32415491 MS.gene056486:CDS
AGCAACACGATTCCGACAAT+TGG 0.337163 8.3:-32416409 None:intergenic
TTGGGAGGACAAGAGCAAAA+AGG 0.339571 8.3:-32416390 None:intergenic
CACAAACTGGTTGGGGAGTT+AGG 0.342051 8.3:+32416472 MS.gene056486:CDS
TATGGTTGTATCGCGATAAA+AGG 0.345751 8.3:+32415542 MS.gene056486:CDS
TGTATAGGATTATTGATTGC+TGG 0.360049 8.3:+32415956 MS.gene056486:CDS
AGCATAGGTTGGAAGTGTTT+AGG 0.365235 8.3:+32416445 MS.gene056486:CDS
ACCTGATATAGATTATTCTA+TGG 0.365689 8.3:+32416689 MS.gene056486:CDS
GCATTACGGGATCGAGATTA+GGG 0.365857 8.3:+32415867 MS.gene056486:CDS
CTAGGAAGATTAAGAAAATT+GGG 0.368395 8.3:+32416073 MS.gene056486:CDS
AGCAAACCCTGAAGATTATA+GGG 0.370075 8.3:-32416333 None:intergenic
TCCATAGAATAATCTATATC+AGG 0.373576 8.3:-32416690 None:intergenic
ATGGTGGAATTATGTGTAAT+TGG 0.382160 8.3:+32415613 MS.gene056486:CDS
TATAGATAGATGGGCAGAAT+GGG 0.382516 8.3:+32416614 MS.gene056486:CDS
CATCAGAGCGGGAAAGCTAT+TGG 0.382523 8.3:+32416246 MS.gene056486:CDS
TAATCTATATCAGGTATTAA+TGG 0.385147 8.3:-32416681 None:intergenic
TCCCGTATACTCGCAGATAA+AGG 0.385176 8.3:-32416520 None:intergenic
TAGGTCTGCACAAACTGGTT+GGG 0.389899 8.3:+32416464 MS.gene056486:CDS
TGCATTACGGGATCGAGATT+AGG 0.391240 8.3:+32415866 MS.gene056486:CDS
TCGGAATCGTGTTGCTCAAA+AGG 0.393717 8.3:+32416416 MS.gene056486:CDS
AGAGGGAGAATGTTCCTATT+AGG 0.395044 8.3:+32416142 MS.gene056486:CDS
AATTCTTCTTTCTTCCTCCT+TGG 0.396457 8.3:-32415783 None:intergenic
ACAAACTGAACAAATACATT+TGG 0.398498 8.3:-32416762 None:intergenic
CTAAATCATAACCTTAATAT+GGG 0.399163 8.3:+32415040 MS.gene056486:CDS
TAAAGGCTCCAGCTTGAATA+AGG 0.402182 8.3:-32416503 None:intergenic
GCTCTTGTCCTCCCAATTGT+CGG 0.405346 8.3:+32416397 MS.gene056486:CDS
GATTCTTGTTTATTTGAAAT+GGG 0.406495 8.3:-32415103 None:intergenic
TATAAGTTATTGAGAGTTGA+AGG 0.409965 8.3:+32416015 MS.gene056486:CDS
GGTAAGGTTTATGTTTATGA+TGG 0.410667 8.3:+32415931 MS.gene056486:CDS
CTGATCACAAATTTGAATTG+AGG 0.414878 8.3:-32415806 None:intergenic
AGAGATTGGTTGAGGAGAAG+AGG 0.415274 8.3:+32415338 MS.gene056486:CDS
AAACTCTCCTCCATTCTTCA+TGG 0.416886 8.3:-32416310 None:intergenic
ATTGGTTGAGGAGAAGAGGT+TGG 0.418514 8.3:+32415342 MS.gene056486:CDS
GGAAGATACTCATAATTAAG+AGG 0.420348 8.3:-32416195 None:intergenic
ATGTTTATGATGGATTGTAT+AGG 0.423249 8.3:+32415941 MS.gene056486:CDS
TGTGGAGGCAGATAAGAGAT+TGG 0.428163 8.3:+32415324 MS.gene056486:CDS
AGAGAACTCGTCATAGACTA+TGG 0.430962 8.3:+32416855 MS.gene056486:CDS
AGAAGAGGTTGGATGAGGAT+AGG 0.436158 8.3:+32415353 MS.gene056486:CDS
CGGAATCGTGTTGCTCAAAA+GGG 0.437967 8.3:+32416417 MS.gene056486:CDS
AGTAGCAATTGGCTCGCCAT+TGG 0.452219 8.3:-32415689 None:intergenic
GTGTTTAGGTCTGCACAAAC+TGG 0.452666 8.3:+32416459 MS.gene056486:CDS
AGGATAGTTGGTTCGATACC+TGG 0.455737 8.3:+32415562 MS.gene056486:CDS
TCAACAGAAAGACAATTCAT+AGG 0.456778 8.3:-32416108 None:intergenic
TGCGATTATGGAGGCGGAGA+TGG 0.457729 8.3:+32415393 MS.gene056486:CDS
TTATGAGGATGATATGGAAT+TGG 0.457955 8.3:+32415732 MS.gene056486:CDS
GGCAGATAAGAGATTGGTTG+AGG 0.458910 8.3:+32415330 MS.gene056486:CDS
GATGATATGGAATTGGATAA+TGG 0.459588 8.3:+32415739 MS.gene056486:CDS
GAGTTTCCCTATAATCTTCA+GGG 0.462595 8.3:+32416327 MS.gene056486:CDS
CTTCTTTCTTCCTCCTTGGC+CGG 0.463064 8.3:-32415779 None:intergenic
ATTGATTGCTGGTTTGAAGA+AGG 0.466360 8.3:+32415967 MS.gene056486:CDS
GTAGCAATTGGCTCGCCATT+GGG 0.469298 8.3:-32415688 None:intergenic
ATCATGTTCGGCAGTAGCTC+TGG 0.471165 8.3:+32415123 MS.gene056486:CDS
ACAATCACACTAGTAGCAAT+TGG 0.471909 8.3:-32415700 None:intergenic
TCCTTGGACCTTATTCAAGC+TGG 0.472407 8.3:+32416495 MS.gene056486:CDS
AGTTGAAGGGCAAACTAAGA+TGG 0.474128 8.3:+32416029 MS.gene056486:CDS
TATCGCGATAAAAGGATAGT+TGG 0.479508 8.3:+32415550 MS.gene056486:CDS
TTAGAGCGTAGTATGCATTA+CGG 0.480635 8.3:+32415853 MS.gene056486:CDS
TAAGGTTATGATTTAGTCCA+AGG 0.481584 8.3:-32415033 None:intergenic
TCAGTCAATATTTCTGACCT+TGG 0.482765 8.3:+32415016 MS.gene056486:CDS
GATTGTTTCAAGTGGTTATG+AGG 0.483946 8.3:+32415717 MS.gene056486:CDS
GCAACACGATTCCGACAATT+GGG 0.484911 8.3:-32416408 None:intergenic
TAGAGCGTAGTATGCATTAC+GGG 0.486228 8.3:+32415854 MS.gene056486:CDS
GAAGATTAAGAAAATTGGGT+TGG 0.487319 8.3:+32416077 MS.gene056486:CDS
TCAGGTATTAATGGATATGA+TGG 0.489556 8.3:-32416672 None:intergenic
TAATGAAGGACCGAGAGTTA+TGG 0.490732 8.3:+32415524 MS.gene056486:CDS
GCCTTTATCTGCGAGTATAC+GGG 0.492679 8.3:+32416519 MS.gene056486:CDS
TTGATTGCTGGTTTGAAGAA+GGG 0.494752 8.3:+32415968 MS.gene056486:CDS
CTTCAGGGTTTGCTTGTGAA+AGG 0.494789 8.3:+32416342 MS.gene056486:CDS
TCATATTCGCGGTGACATAT+GGG 0.505990 8.3:+32415492 MS.gene056486:CDS
TGTGTGGAGGCTTGTACTGA+TGG 0.507598 8.3:+32416276 MS.gene056486:CDS
TGAGGAGAAGAGGTTGGATG+AGG 0.507616 8.3:+32415348 MS.gene056486:CDS
AGGTCTGCACAAACTGGTTG+GGG 0.507788 8.3:+32416465 MS.gene056486:CDS
AAATAAACAAGAATCATGTT+CGG 0.510967 8.3:+32415111 MS.gene056486:CDS
TAGATAGATGGGCAGAATGG+GGG 0.511424 8.3:+32416616 MS.gene056486:CDS
GCGATTATGGAGGCGGAGAT+GGG 0.511508 8.3:+32415394 MS.gene056486:CDS
ACATCAGATTGTTATGATCA+TGG 0.511816 8.3:-32416788 None:intergenic
TTATAGATAGATGGGCAGAA+TGG 0.518603 8.3:+32416613 MS.gene056486:CDS
ATGGAGGCGGAGATGGGAGC+TGG 0.518912 8.3:+32415400 MS.gene056486:CDS
GGCATCCCTGACGAGTGGGC+GGG 0.520972 8.3:+32416876 MS.gene056486:CDS
AGATAGATGGGCAGAATGGG+GGG 0.535500 8.3:+32416617 MS.gene056486:CDS
AGACGTTATAATCATATTCG+CGG 0.536023 8.3:+32415481 MS.gene056486:CDS
TTCGTGGGATGAAGTATGAA+GGG 0.537787 8.3:+32415893 MS.gene056486:CDS
TGGTTTGAAGAAGGGATGAA+TGG 0.538003 8.3:+32415976 MS.gene056486:CDS
TGAAGTTGCGATTATGGAGG+CGG 0.538130 8.3:+32415387 MS.gene056486:CDS
GGTGATGTAATAATCTATAC+CGG 0.538669 8.3:+32415760 MS.gene056486:CDS
AATTTGTGATCAGAAATTAG+AGG 0.546612 8.3:+32415816 MS.gene056486:CDS
AAAGGGGATCAAGCATAGGT+TGG 0.547811 8.3:+32416434 MS.gene056486:CDS
TCGATACCTGGAGTTTATGT+TGG 0.551588 8.3:+32415574 MS.gene056486:CDS
ATAAGTTATTGAGAGTTGAA+GGG 0.555115 8.3:+32416016 MS.gene056486:CDS
GGTTTGTATTTCATCAGAGC+GGG 0.557067 8.3:+32416235 MS.gene056486:CDS
CAAATATGAGTATGAAGGAT+CGG 0.557193 8.3:+32415182 MS.gene056486:CDS
ATGGAGGGCGAAATGTTGAA+TGG 0.558277 8.3:+32415454 MS.gene056486:CDS
ATCGAGATTAGGGTTATTCG+TGG 0.558682 8.3:+32415877 MS.gene056486:CDS
AGAGGCAGCAACATGGATGG+AGG 0.559470 8.3:+32415438 MS.gene056486:CDS
CCGTCTCATGCTATATGCAA+TGG 0.560658 8.3:+32416815 MS.gene056486:CDS
TGTTGATATTTCACACAAGA+GGG 0.562610 8.3:+32416125 MS.gene056486:CDS
CGCGATACAACCATAACTCT+CGG 0.563453 8.3:-32415534 None:intergenic
TTAGGTCTGCACAAACTGGT+TGG 0.564740 8.3:+32416463 MS.gene056486:CDS
TGGTGATGAGAACATGGAAG+AGG 0.565897 8.3:+32415420 MS.gene056486:CDS
GGGTGCGTCTAGCTCTGGTA+AGG 0.568302 8.3:+32415915 MS.gene056486:CDS
TGGCATCCCTGACGAGTGGG+CGG 0.569853 8.3:+32416875 MS.gene056486:CDS
ATTCGTGGGATGAAGTATGA+AGG 0.571493 8.3:+32415892 MS.gene056486:CDS
CTGTTGATATTTCACACAAG+AGG 0.573952 8.3:+32416124 MS.gene056486:CDS
CATTGCATATAGCATGAGAC+GGG 0.574005 8.3:-32416814 None:intergenic
ATAGATAGATGGGCAGAATG+GGG 0.576328 8.3:+32416615 MS.gene056486:CDS
GTGTTGTTCTCAAAGATGCT+AGG 0.577721 8.3:+32416055 MS.gene056486:CDS
ATTTGTGATCAGAAATTAGA+GGG 0.579266 8.3:+32415817 MS.gene056486:CDS
ATGGGCTGCGAGGTTAATGA+AGG 0.580888 8.3:+32415510 MS.gene056486:CDS
GGAAGAGGCAGCAACATGGA+TGG 0.581307 8.3:+32415435 MS.gene056486:CDS
GCTTGCCCGCCCACTCGTCA+GGG 0.582498 8.3:-32416881 None:intergenic
TTGCATATGCAGATACAAGC+TGG 0.583868 8.3:+32415637 MS.gene056486:CDS
GTAATAATCTATACCGGCCA+AGG 0.586629 8.3:+32415766 MS.gene056486:CDS
CCATTGCATATAGCATGAGA+CGG 0.588288 8.3:-32416815 None:intergenic
GTATTCAAATATGAGTATGA+AGG 0.592101 8.3:+32415177 MS.gene056486:CDS
GCAACGTGATGAGGAGAACA+AGG 0.596580 8.3:+32415215 MS.gene056486:CDS
AGCTATTGGCTGTGAATGTG+TGG 0.596741 8.3:+32416260 MS.gene056486:CDS
CTCTGATGAAATACAAACCA+CGG 0.598765 8.3:-32416231 None:intergenic
ACATCACCAACATAAACTCC+AGG 0.599500 8.3:-32415580 None:intergenic
TGGTTGGGGAGTTAGGTCCT+TGG 0.600362 8.3:+32416479 MS.gene056486:CDS
GGGAGCTGGTGATGAGAACA+TGG 0.604987 8.3:+32415414 MS.gene056486:CDS
GTTGAAGGGCAAACTAAGAT+GGG 0.605405 8.3:+32416030 MS.gene056486:CDS
GCATACTACGCTCTAAAGCA+AGG 0.606571 8.3:-32415846 None:intergenic
TCTATGACGAGTTCTCTCAA+TGG 0.607917 8.3:-32416849 None:intergenic
ATGACGAGTTCTCTCAATGG+AGG 0.609086 8.3:-32416846 None:intergenic
TAAATCATAACCTTAATATG+GGG 0.609176 8.3:+32415041 MS.gene056486:CDS
ATACATTTGGACTCGAACTG+TGG 0.609812 8.3:-32416749 None:intergenic
ACTAGTGTGATTGTTTCAAG+TGG 0.613031 8.3:+32415709 MS.gene056486:CDS
ACGTGTGTTAGCAAGTATTG+AGG 0.613466 8.3:+32415255 MS.gene056486:CDS
AGGCAAGTCAGATGCTTGTG+AGG 0.614350 8.3:+32415155 MS.gene056486:CDS
TCGTGGGATGAAGTATGAAG+GGG 0.618537 8.3:+32415894 MS.gene056486:CDS
TCCAGCTTGAATAAGGTCCA+AGG 0.620502 8.3:-32416496 None:intergenic
TTCTGAAGTTGCGATTATGG+AGG 0.624607 8.3:+32415384 MS.gene056486:CDS
CTATGGCATCCCTGACGAGT+GGG 0.626017 8.3:+32416872 MS.gene056486:CDS
GAGGCAGCAACATGGATGGA+GGG 0.628691 8.3:+32415439 MS.gene056486:CDS
ACATGGAAGAGGCAGCAACA+TGG 0.634502 8.3:+32415431 MS.gene056486:CDS
TCGAGATTAGGGTTATTCGT+GGG 0.635248 8.3:+32415878 MS.gene056486:CDS
CTCAAAAGGGGATCAAGCAT+AGG 0.636242 8.3:+32416430 MS.gene056486:CDS
TGTGATCAGAAATTAGAGGG+TGG 0.640509 8.3:+32415820 MS.gene056486:CDS
TGACGAGTTCTCTCAATGGA+GGG 0.642236 8.3:-32416845 None:intergenic
AAATCATAACCTTAATATGG+GGG 0.649898 8.3:+32415042 MS.gene056486:CDS
ACTATGGCATCCCTGACGAG+TGG 0.651275 8.3:+32416871 MS.gene056486:CDS
GGAATCGTGTTGCTCAAAAG+GGG 0.665750 8.3:+32416418 MS.gene056486:CDS
TGGTTTGTATTTCATCAGAG+CGG 0.676509 8.3:+32416234 MS.gene056486:CDS
ACACGATTCCGACAATTGGG+AGG 0.682616 8.3:-32416405 None:intergenic
TATTGGCTGTGAATGTGTGG+AGG 0.683730 8.3:+32416263 MS.gene056486:CDS
CCTAAAAGTAGGTCTCCCAA+TGG 0.692154 8.3:+32415673 MS.gene056486:CDS
CGTGGGATGAAGTATGAAGG+GGG 0.704890 8.3:+32415895 MS.gene056486:CDS
ATAATCTATACCGGCCAAGG+AGG 0.705547 8.3:+32415769 MS.gene056486:CDS
ATTGCATATAGCATGAGACG+GGG 0.721884 8.3:-32416813 None:intergenic
TTGCATATAGCATGAGACGG+GGG 0.722787 8.3:-32416812 None:intergenic
GCGGTGACATATGGGCTGCG+AGG 0.738337 8.3:+32415500 MS.gene056486:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGTTTATAGATAGAT+GGG + chr8.3:32416605-32416624 MS.gene056486:CDS 20.0%
!! AAATAAACAAGAATCATGTT+CGG + chr8.3:32415111-32415130 MS.gene056486:CDS 20.0%
!! ACTAAATCATAACCTTAATA+TGG + chr8.3:32415039-32415058 MS.gene056486:CDS 20.0%
!! CTAAATCATAACCTTAATAT+GGG + chr8.3:32415040-32415059 MS.gene056486:CDS 20.0%
!! TAAATCATAACCTTAATATG+GGG + chr8.3:32415041-32415060 MS.gene056486:CDS 20.0%
!! TAATCTATATCAGGTATTAA+TGG - chr8.3:32416684-32416703 None:intergenic 20.0%
!!! GATTCTTGTTTATTTGAAAT+GGG - chr8.3:32415106-32415125 None:intergenic 20.0%
!!! TGATTCTTGTTTATTTGAAA+TGG - chr8.3:32415107-32415126 None:intergenic 20.0%
! AAATCATAACCTTAATATGG+GGG + chr8.3:32415042-32415061 MS.gene056486:CDS 25.0%
! AATTATTGAGGAGAAGAAAT+TGG + chr8.3:32415288-32415307 MS.gene056486:CDS 25.0%
! AATTTGTGATCAGAAATTAG+AGG + chr8.3:32415816-32415835 MS.gene056486:CDS 25.0%
! ACAAACTGAACAAATACATT+TGG - chr8.3:32416765-32416784 None:intergenic 25.0%
! ACCTGATATAGATTATTCTA+TGG + chr8.3:32416689-32416708 MS.gene056486:CDS 25.0%
! ACTCGTTAATATATCCAAAA+AGG + chr8.3:32416589-32416608 MS.gene056486:CDS 25.0%
! ATAAGTTATTGAGAGTTGAA+GGG + chr8.3:32416016-32416035 MS.gene056486:CDS 25.0%
! ATGTCATTAAATAGCCTAAT+AGG - chr8.3:32416159-32416178 None:intergenic 25.0%
! ATTTGTGATCAGAAATTAGA+GGG + chr8.3:32415817-32415836 MS.gene056486:CDS 25.0%
! CAAAAAGGTTTATAGATAGA+TGG + chr8.3:32416604-32416623 MS.gene056486:CDS 25.0%
! CTAGGAAGATTAAGAAAATT+GGG + chr8.3:32416073-32416092 MS.gene056486:CDS 25.0%
! GGATGAGAAAAAAATTATTG+AGG + chr8.3:32415276-32415295 MS.gene056486:CDS 25.0%
! GTATTCAAATATGAGTATGA+AGG + chr8.3:32415177-32415196 MS.gene056486:CDS 25.0%
! TATAAGTTATTGAGAGTTGA+AGG + chr8.3:32416015-32416034 MS.gene056486:CDS 25.0%
! TCCATAGAATAATCTATATC+AGG - chr8.3:32416693-32416712 None:intergenic 25.0%
!! CATCTATCTATAAACCTTTT+TGG - chr8.3:32416606-32416625 None:intergenic 25.0%
!! TTGATTTTCTTCCTAAAAGT+AGG + chr8.3:32415662-32415681 MS.gene056486:CDS 25.0%
!!! ATGTTTATGATGGATTGTAT+AGG + chr8.3:32415941-32415960 MS.gene056486:CDS 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG + chr8.3:32416564-32416583 MS.gene056486:CDS 25.0%
ACATCAGATTGTTATGATCA+TGG - chr8.3:32416791-32416810 None:intergenic 30.0%
AGACGTTATAATCATATTCG+CGG + chr8.3:32415481-32415500 MS.gene056486:CDS 30.0%
CAAATATGAGTATGAAGGAT+CGG + chr8.3:32415182-32415201 MS.gene056486:CDS 30.0%
CTGATCACAAATTTGAATTG+AGG - chr8.3:32415809-32415828 None:intergenic 30.0%
GAAGATTAAGAAAATTGGGT+TGG + chr8.3:32416077-32416096 MS.gene056486:CDS 30.0%
GATGATATGGAATTGGATAA+TGG + chr8.3:32415739-32415758 MS.gene056486:CDS 30.0%
GCTAGGAAGATTAAGAAAAT+TGG + chr8.3:32416072-32416091 MS.gene056486:CDS 30.0%
GGAAGATACTCATAATTAAG+AGG - chr8.3:32416198-32416217 None:intergenic 30.0%
GGTAAGGTTTATGTTTATGA+TGG + chr8.3:32415931-32415950 MS.gene056486:CDS 30.0%
GGTGATGTAATAATCTATAC+CGG + chr8.3:32415760-32415779 MS.gene056486:CDS 30.0%
TAAGGTTATGATTTAGTCCA+AGG - chr8.3:32415036-32415055 None:intergenic 30.0%
TCAACAGAAAGACAATTCAT+AGG - chr8.3:32416111-32416130 None:intergenic 30.0%
TGTTGATATTTCACACAAGA+GGG + chr8.3:32416125-32416144 MS.gene056486:CDS 30.0%
TTATGAGGATGATATGGAAT+TGG + chr8.3:32415732-32415751 MS.gene056486:CDS 30.0%
! AACCACGGAAAAATAGTTTT+TGG - chr8.3:32416219-32416238 None:intergenic 30.0%
! ATGGTGGAATTATGTGTAAT+TGG + chr8.3:32415613-32415632 MS.gene056486:CDS 30.0%
! TCAGGTATTAATGGATATGA+TGG - chr8.3:32416675-32416694 None:intergenic 30.0%
! TGATGTTTTCTTGTATATGG+TGG + chr8.3:32415597-32415616 MS.gene056486:CDS 30.0%
! TGTATAGGATTATTGATTGC+TGG + chr8.3:32415956-32415975 MS.gene056486:CDS 30.0%
!! AAGCCTTTGATTTTTGAATG+TGG + chr8.3:32416366-32416385 MS.gene056486:CDS 30.0%
!! ATACGGGAGTAATTTTAACA+AGG + chr8.3:32416535-32416554 MS.gene056486:CDS 30.0%
!! TACGGGAGTAATTTTAACAA+GGG + chr8.3:32416536-32416555 MS.gene056486:CDS 30.0%
!! TGGTGATGTTTTCTTGTATA+TGG + chr8.3:32415594-32415613 MS.gene056486:CDS 30.0%
!! TTCCAAAAACTATTTTTCCG+TGG + chr8.3:32416214-32416233 MS.gene056486:CDS 30.0%
AAGCAAACCCTGAAGATTAT+AGG - chr8.3:32416337-32416356 None:intergenic 35.0%
AAGTGGTTATGAGGATGATA+TGG + chr8.3:32415726-32415745 MS.gene056486:CDS 35.0%
AATTCTTCTTTCTTCCTCCT+TGG - chr8.3:32415786-32415805 None:intergenic 35.0%
ACAATCACACTAGTAGCAAT+TGG - chr8.3:32415703-32415722 None:intergenic 35.0%
ACTAGTGTGATTGTTTCAAG+TGG + chr8.3:32415709-32415728 MS.gene056486:CDS 35.0%
AGAGTTTCCCTATAATCTTC+AGG + chr8.3:32416326-32416345 MS.gene056486:CDS 35.0%
AGCAAACCCTGAAGATTATA+GGG - chr8.3:32416336-32416355 None:intergenic 35.0%
CTCTGATGAAATACAAACCA+CGG - chr8.3:32416234-32416253 None:intergenic 35.0%
CTGTTGATATTTCACACAAG+AGG + chr8.3:32416124-32416143 MS.gene056486:CDS 35.0%
GAGTTTCCCTATAATCTTCA+GGG + chr8.3:32416327-32416346 MS.gene056486:CDS 35.0%
TATAGATAGATGGGCAGAAT+GGG + chr8.3:32416614-32416633 MS.gene056486:CDS 35.0%
TATCGCGATAAAAGGATAGT+TGG + chr8.3:32415550-32415569 MS.gene056486:CDS 35.0%
TATGGTTGTATCGCGATAAA+AGG + chr8.3:32415542-32415561 MS.gene056486:CDS 35.0%
TTAGAGCGTAGTATGCATTA+CGG + chr8.3:32415853-32415872 MS.gene056486:CDS 35.0%
TTATAGATAGATGGGCAGAA+TGG + chr8.3:32416613-32416632 MS.gene056486:CDS 35.0%
! GATTGTTTCAAGTGGTTATG+AGG + chr8.3:32415717-32415736 MS.gene056486:CDS 35.0%
! TCAGTCAATATTTCTGACCT+TGG + chr8.3:32415016-32415035 MS.gene056486:CDS 35.0%
! TGGTTTGTATTTCATCAGAG+CGG + chr8.3:32416234-32416253 MS.gene056486:CDS 35.0%
!! AGTAGCTCTGGTTAAGAAAA+AGG + chr8.3:32415135-32415154 MS.gene056486:CDS 35.0%
!! ATTGATTGCTGGTTTGAAGA+AGG + chr8.3:32415967-32415986 MS.gene056486:CDS 35.0%
!! TGTTTCTGAAGTTGCGATTA+TGG + chr8.3:32415381-32415400 MS.gene056486:CDS 35.0%
!! TTGATTGCTGGTTTGAAGAA+GGG + chr8.3:32415968-32415987 MS.gene056486:CDS 35.0%
AAACTCTCCTCCATTCTTCA+TGG - chr8.3:32416313-32416332 None:intergenic 40.0%
ACATCACCAACATAAACTCC+AGG - chr8.3:32415583-32415602 None:intergenic 40.0%
AGAGAACTCGTCATAGACTA+TGG + chr8.3:32416855-32416874 MS.gene056486:CDS 40.0%
AGAGGGAGAATGTTCCTATT+AGG + chr8.3:32416142-32416161 MS.gene056486:CDS 40.0%
AGCCTTTATCTGCGAGTATA+CGG + chr8.3:32416518-32416537 MS.gene056486:CDS 40.0%
AGTTGAAGGGCAAACTAAGA+TGG + chr8.3:32416029-32416048 MS.gene056486:CDS 40.0%
ATACATTTGGACTCGAACTG+TGG - chr8.3:32416752-32416771 None:intergenic 40.0%
ATAGATAGATGGGCAGAATG+GGG + chr8.3:32416615-32416634 MS.gene056486:CDS 40.0%
ATCATATTCGCGGTGACATA+TGG + chr8.3:32415491-32415510 MS.gene056486:CDS 40.0%
ATCGAGATTAGGGTTATTCG+TGG + chr8.3:32415877-32415896 MS.gene056486:CDS 40.0%
ATTCGTGGGATGAAGTATGA+AGG + chr8.3:32415892-32415911 MS.gene056486:CDS 40.0%
ATTGCATATAGCATGAGACG+GGG - chr8.3:32416816-32416835 None:intergenic 40.0%
CAAAATCCACCCCCATATTA+AGG - chr8.3:32415054-32415073 None:intergenic 40.0%
CATTGCATATAGCATGAGAC+GGG - chr8.3:32416817-32416836 None:intergenic 40.0%
CCATTGCATATAGCATGAGA+CGG - chr8.3:32416818-32416837 None:intergenic 40.0%
GGGCCACATTCAAAAATCAA+AGG - chr8.3:32416372-32416391 None:intergenic 40.0%
GGTTTGTATTTCATCAGAGC+GGG + chr8.3:32416235-32416254 MS.gene056486:CDS 40.0%
GTAATAATCTATACCGGCCA+AGG + chr8.3:32415766-32415785 MS.gene056486:CDS 40.0%
GTTGAAGGGCAAACTAAGAT+GGG + chr8.3:32416030-32416049 MS.gene056486:CDS 40.0%
TAAAGGCTCCAGCTTGAATA+AGG - chr8.3:32416506-32416525 None:intergenic 40.0%
TAATGAAGGACCGAGAGTTA+TGG + chr8.3:32415524-32415543 MS.gene056486:CDS 40.0%
TAGAGCGTAGTATGCATTAC+GGG + chr8.3:32415854-32415873 MS.gene056486:CDS 40.0%
TCATAACCTTAATATGGGGG+TGG + chr8.3:32415045-32415064 MS.gene056486:CDS 40.0%
TCATATTCGCGGTGACATAT+GGG + chr8.3:32415492-32415511 MS.gene056486:CDS 40.0%
TCGAGATTAGGGTTATTCGT+GGG + chr8.3:32415878-32415897 MS.gene056486:CDS 40.0%
TCGATACCTGGAGTTTATGT+TGG + chr8.3:32415574-32415593 MS.gene056486:CDS 40.0%
TCTATGACGAGTTCTCTCAA+TGG - chr8.3:32416852-32416871 None:intergenic 40.0%
TGGTTTGAAGAAGGGATGAA+TGG + chr8.3:32415976-32415995 MS.gene056486:CDS 40.0%
TGTGATCAGAAATTAGAGGG+TGG + chr8.3:32415820-32415839 MS.gene056486:CDS 40.0%
TTCGTGGGATGAAGTATGAA+GGG + chr8.3:32415893-32415912 MS.gene056486:CDS 40.0%
TTGCATATGCAGATACAAGC+TGG + chr8.3:32415637-32415656 MS.gene056486:CDS 40.0%
! ACGTGTGTTAGCAAGTATTG+AGG + chr8.3:32415255-32415274 MS.gene056486:CDS 40.0%
! ATTGGCTGACGAAGTTTTTG+TGG + chr8.3:32415306-32415325 MS.gene056486:CDS 40.0%
! GTGTTGTTCTCAAAGATGCT+AGG + chr8.3:32416055-32416074 MS.gene056486:CDS 40.0%
! TGCATTTTTGCAACGTGATG+AGG + chr8.3:32415206-32415225 MS.gene056486:CDS 40.0%
! TGCTTTTGCTCCATGAAGAA+TGG + chr8.3:32416300-32416319 MS.gene056486:CDS 40.0%
! TTTTGCTCCATGAAGAATGG+AGG + chr8.3:32416303-32416322 MS.gene056486:CDS 40.0%
!! AGCATAGGTTGGAAGTGTTT+AGG + chr8.3:32416445-32416464 MS.gene056486:CDS 40.0%
!! TAATATGGGGGTGGATTTTG+TGG + chr8.3:32415054-32415073 MS.gene056486:CDS 40.0%
!! TTCTGAAGTTGCGATTATGG+AGG + chr8.3:32415384-32415403 MS.gene056486:CDS 40.0%
AAAGGGGATCAAGCATAGGT+TGG + chr8.3:32416434-32416453 MS.gene056486:CDS 45.0%
AGAAGAGGTTGGATGAGGAT+AGG + chr8.3:32415353-32415372 MS.gene056486:CDS 45.0%
AGCAACACGATTCCGACAAT+TGG - chr8.3:32416412-32416431 None:intergenic 45.0%
ATAATCTATACCGGCCAAGG+AGG + chr8.3:32415769-32415788 MS.gene056486:CDS 45.0%
ATGACGAGTTCTCTCAATGG+AGG - chr8.3:32416849-32416868 None:intergenic 45.0%
ATGGAGGGCGAAATGTTGAA+TGG + chr8.3:32415454-32415473 MS.gene056486:CDS 45.0%
ATTGGTTGAGGAGAAGAGGT+TGG + chr8.3:32415342-32415361 MS.gene056486:CDS 45.0%
CCGTCTCATGCTATATGCAA+TGG + chr8.3:32416815-32416834 MS.gene056486:CDS 45.0%
CCTAAAAGTAGGTCTCCCAA+TGG + chr8.3:32415673-32415692 MS.gene056486:CDS 45.0%
CGCGATACAACCATAACTCT+CGG - chr8.3:32415537-32415556 None:intergenic 45.0%
CTCAAAAGGGGATCAAGCAT+AGG + chr8.3:32416430-32416449 MS.gene056486:CDS 45.0%
GCAACACGATTCCGACAATT+GGG - chr8.3:32416411-32416430 None:intergenic 45.0%
GCATTACGGGATCGAGATTA+GGG + chr8.3:32415867-32415886 MS.gene056486:CDS 45.0%
GCCTTTATCTGCGAGTATAC+GGG + chr8.3:32416519-32416538 MS.gene056486:CDS 45.0%
GTGTTTAGGTCTGCACAAAC+TGG + chr8.3:32416459-32416478 MS.gene056486:CDS 45.0%
TAGATAGATGGGCAGAATGG+GGG + chr8.3:32416616-32416635 MS.gene056486:CDS 45.0%
TAGGTCTGCACAAACTGGTT+GGG + chr8.3:32416464-32416483 MS.gene056486:CDS 45.0%
TCCAGCTTGAATAAGGTCCA+AGG - chr8.3:32416499-32416518 None:intergenic 45.0%
TCCCGTATACTCGCAGATAA+AGG - chr8.3:32416523-32416542 None:intergenic 45.0%
TCCTTGGACCTTATTCAAGC+TGG + chr8.3:32416495-32416514 MS.gene056486:CDS 45.0%
TCGTGGGATGAAGTATGAAG+GGG + chr8.3:32415894-32415913 MS.gene056486:CDS 45.0%
TGAAGTTGCGATTATGGAGG+CGG + chr8.3:32415387-32415406 MS.gene056486:CDS 45.0%
TGACGAGTTCTCTCAATGGA+GGG - chr8.3:32416848-32416867 None:intergenic 45.0%
TGCATTACGGGATCGAGATT+AGG + chr8.3:32415866-32415885 MS.gene056486:CDS 45.0%
TGGGAGGACAAGAGCAAAAA+GGG - chr8.3:32416392-32416411 None:intergenic 45.0%
TGTGGAGGCAGATAAGAGAT+TGG + chr8.3:32415324-32415343 MS.gene056486:CDS 45.0%
TTAGGTCTGCACAAACTGGT+TGG + chr8.3:32416463-32416482 MS.gene056486:CDS 45.0%
TTGCATATAGCATGAGACGG+GGG - chr8.3:32416815-32416834 None:intergenic 45.0%
TTGGGAGGACAAGAGCAAAA+AGG - chr8.3:32416393-32416412 None:intergenic 45.0%
! AGAGATTGGTTGAGGAGAAG+AGG + chr8.3:32415338-32415357 MS.gene056486:CDS 45.0%
! AGCTATTGGCTGTGAATGTG+TGG + chr8.3:32416260-32416279 MS.gene056486:CDS 45.0%
! CGGAATCGTGTTGCTCAAAA+GGG + chr8.3:32416417-32416436 MS.gene056486:CDS 45.0%
! CTTCAGGGTTTGCTTGTGAA+AGG + chr8.3:32416342-32416361 MS.gene056486:CDS 45.0%
! GGAATCGTGTTGCTCAAAAG+GGG + chr8.3:32416418-32416437 MS.gene056486:CDS 45.0%
! GGCAGATAAGAGATTGGTTG+AGG + chr8.3:32415330-32415349 MS.gene056486:CDS 45.0%
! TATTGGCTGTGAATGTGTGG+AGG + chr8.3:32416263-32416282 MS.gene056486:CDS 45.0%
! TCGGAATCGTGTTGCTCAAA+AGG + chr8.3:32416416-32416435 MS.gene056486:CDS 45.0%
! TGGTGATGAGAACATGGAAG+AGG + chr8.3:32415420-32415439 MS.gene056486:CDS 45.0%
!! AGGATAGTTGGTTCGATACC+TGG + chr8.3:32415562-32415581 MS.gene056486:CDS 45.0%
!! CCATTGGGAGACCTACTTTT+AGG - chr8.3:32415676-32415695 None:intergenic 45.0%
!! GCATACTACGCTCTAAAGCA+AGG - chr8.3:32415849-32415868 None:intergenic 45.0%
ACACGATTCCGACAATTGGG+AGG - chr8.3:32416408-32416427 None:intergenic 50.0%
ACATGGAAGAGGCAGCAACA+TGG + chr8.3:32415431-32415450 MS.gene056486:CDS 50.0%
AGATAGATGGGCAGAATGGG+GGG + chr8.3:32416617-32416636 MS.gene056486:CDS 50.0%
AGGCAAGTCAGATGCTTGTG+AGG + chr8.3:32415155-32415174 MS.gene056486:CDS 50.0%
AGGTCTGCACAAACTGGTTG+GGG + chr8.3:32416465-32416484 MS.gene056486:CDS 50.0%
ATCATGTTCGGCAGTAGCTC+TGG + chr8.3:32415123-32415142 MS.gene056486:CDS 50.0%
CACAAACTGGTTGGGGAGTT+AGG + chr8.3:32416472-32416491 MS.gene056486:CDS 50.0%
CATCAGAGCGGGAAAGCTAT+TGG + chr8.3:32416246-32416265 MS.gene056486:CDS 50.0%
CGTGGGATGAAGTATGAAGG+GGG + chr8.3:32415895-32415914 MS.gene056486:CDS 50.0%
CTTCTTTCTTCCTCCTTGGC+CGG - chr8.3:32415782-32415801 None:intergenic 50.0%
GCAACGTGATGAGGAGAACA+AGG + chr8.3:32415215-32415234 MS.gene056486:CDS 50.0%
GCTCTTGTCCTCCCAATTGT+CGG + chr8.3:32416397-32416416 MS.gene056486:CDS 50.0%
TGAGGAGAAGAGGTTGGATG+AGG + chr8.3:32415348-32415367 MS.gene056486:CDS 50.0%
TGTGTGGAGGCTTGTACTGA+TGG + chr8.3:32416276-32416295 MS.gene056486:CDS 50.0%
! ATGGGCTGCGAGGTTAATGA+AGG + chr8.3:32415510-32415529 MS.gene056486:CDS 50.0%
! GGCTGACGAAGTTTTTGTGG+AGG + chr8.3:32415309-32415328 MS.gene056486:CDS 50.0%
!! AGTAGCAATTGGCTCGCCAT+TGG - chr8.3:32415692-32415711 None:intergenic 50.0%
!! GTAGCAATTGGCTCGCCATT+GGG - chr8.3:32415691-32415710 None:intergenic 50.0%
ACTATGGCATCCCTGACGAG+TGG + chr8.3:32416871-32416890 MS.gene056486:CDS 55.0%
AGAGGCAGCAACATGGATGG+AGG + chr8.3:32415438-32415457 MS.gene056486:CDS 55.0%
CTATGGCATCCCTGACGAGT+GGG + chr8.3:32416872-32416891 MS.gene056486:CDS 55.0%
GAGGCAGCAACATGGATGGA+GGG + chr8.3:32415439-32415458 MS.gene056486:CDS 55.0%
GCGATTATGGAGGCGGAGAT+GGG + chr8.3:32415394-32415413 MS.gene056486:CDS 55.0%
GGAAGAGGCAGCAACATGGA+TGG + chr8.3:32415435-32415454 MS.gene056486:CDS 55.0%
TGCGATTATGGAGGCGGAGA+TGG + chr8.3:32415393-32415412 MS.gene056486:CDS 55.0%
TGGTTGGGGAGTTAGGTCCT+TGG + chr8.3:32416479-32416498 MS.gene056486:CDS 55.0%
! GGGAGCTGGTGATGAGAACA+TGG + chr8.3:32415414-32415433 MS.gene056486:CDS 55.0%
!! GGGTGCGTCTAGCTCTGGTA+AGG + chr8.3:32415915-32415934 MS.gene056486:CDS 60.0%
ATGGAGGCGGAGATGGGAGC+TGG + chr8.3:32415400-32415419 MS.gene056486:CDS 65.0%
TGGCATCCCTGACGAGTGGG+CGG + chr8.3:32416875-32416894 MS.gene056486:CDS 65.0%
! GAAGGGGGTGCGTCTAGCTC+TGG + chr8.3:32415910-32415929 MS.gene056486:CDS 65.0%
! GCGGTGACATATGGGCTGCG+AGG + chr8.3:32415500-32415519 MS.gene056486:CDS 65.0%
AGCTTGCCCGCCCACTCGTC+AGG - chr8.3:32416885-32416904 None:intergenic 70.0%
GCTTGCCCGCCCACTCGTCA+GGG - chr8.3:32416884-32416903 None:intergenic 70.0%
GGCATCCCTGACGAGTGGGC+GGG + chr8.3:32416876-32416895 MS.gene056486:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 32415007 32416920 32415007 ID=MS.gene056486
chr8.3 mRNA 32415007 32416920 32415007 ID=MS.gene056486.t1;Parent=MS.gene056486
chr8.3 exon 32415007 32416920 32415007 ID=MS.gene056486.t1.exon1;Parent=MS.gene056486.t1
chr8.3 CDS 32415007 32416920 32415007 ID=cds.MS.gene056486.t1;Parent=MS.gene056486.t1
Gene Sequence

>MS.gene056486

ATGGAGAACTCAGTCAATATTTCTGACCTTGGACTAAATCATAACCTTAATATGGGGGTGGATTTTGTGGATGATAGAAATGATCGATCTATTGTGCCCATTTCAAATAAACAAGAATCATGTTCGGCAGTAGCTCTGGTTAAGAAAAAGGCAAGTCAGATGCTTGTGAGGTATTCAAATATGAGTATGAAGGATCGGATGCATTTTTGCAACGTGATGAGGAGAACAAGGATGACTTTTGATTCGCTACGTGTGTTAGCAAGTATTGAGGATGAGAAAAAAATTATTGAGGAGAAGAAATTGGCTGACGAAGTTTTTGTGGAGGCAGATAAGAGATTGGTTGAGGAGAAGAGGTTGGATGAGGATAGGATATTTGTTTCTGAAGTTGCGATTATGGAGGCGGAGATGGGAGCTGGTGATGAGAACATGGAAGAGGCAGCAACATGGATGGAGGGCGAAATGTTGAATGGAAAAAGACGTTATAATCATATTCGCGGTGACATATGGGCTGCGAGGTTAATGAAGGACCGAGAGTTATGGTTGTATCGCGATAAAAGGATAGTTGGTTCGATACCTGGAGTTTATGTTGGTGATGTTTTCTTGTATATGGTGGAATTATGTGTAATTGGTTTGCATATGCAGATACAAGCTGGCATTGATTTTCTTCCTAAAAGTAGGTCTCCCAATGGCGAGCCAATTGCTACTAGTGTGATTGTTTCAAGTGGTTATGAGGATGATATGGAATTGGATAATGGTGATGTAATAATCTATACCGGCCAAGGAGGAAGAAAGAAGAATTCCTCAATTCAAATTTGTGATCAGAAATTAGAGGGTGGAAACCTTGCTTTAGAGCGTAGTATGCATTACGGGATCGAGATTAGGGTTATTCGTGGGATGAAGTATGAAGGGGGTGCGTCTAGCTCTGGTAAGGTTTATGTTTATGATGGATTGTATAGGATTATTGATTGCTGGTTTGAAGAAGGGATGAATGGTTTTAGAGTTTTTAAGTATAAGTTATTGAGAGTTGAAGGGCAAACTAAGATGGGTAGTGTTGTTCTCAAAGATGCTAGGAAGATTAAGAAAATTGGGTTGGATGTTAAGCCTATGAATTGTCTTTCTGTTGATATTTCACACAAGAGGGAGAATGTTCCTATTAGGCTATTTAATGACATAGATGACAATGAAGAACCTCTTAATTATGAGTATCTTCCAAAAACTATTTTTCCGTGGTTTGTATTTCATCAGAGCGGGAAAGCTATTGGCTGTGAATGTGTGGAGGCTTGTACTGATGGCTGCTTTTGCTCCATGAAGAATGGAGGAGAGTTTCCCTATAATCTTCAGGGTTTGCTTGTGAAAGGTAAGCCTTTGATTTTTGAATGTGGCCCTTTTTGCTCTTGTCCTCCCAATTGTCGGAATCGTGTTGCTCAAAAGGGGATCAAGCATAGGTTGGAAGTGTTTAGGTCTGCACAAACTGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAAGCTGGAGCCTTTATCTGCGAGTATACGGGAGTAATTTTAACAAGGGAACAAGCTCAAATTTTAACAATGAATGGCGACTCGTTAATATATCCAAAAAGGTTTATAGATAGATGGGCAGAATGGGGGGATTTGTCTCAAATATACAATAATTATGTTCGTCCATCATATCCATTAATACCTGATATAGATTATTCTATGGATATATCAACACTGCGAAATGTTGCTAGCTATTTGAGCCACAGTTCGAGTCCAAATGTATTTGTTCAGTTTGTTCTCCATGATCATAACAATCTGATGTTCCCCCGTCTCATGCTATATGCAATGGAGAACATCCCTCCATTGAGAGAACTCGTCATAGACTATGGCATCCCTGACGAGTGGGCGGGCAAGCTATCAGTATGTATGTAA
Protein sequence

>MS.gene056486.t1

MENSVNISDLGLNHNLNMGVDFVDDRNDRSIVPISNKQESCSAVALVKKKASQMLVRYSNMSMKDRMHFCNVMRRTRMTFDSLRVLASIEDEKKIIEEKKLADEVFVEADKRLVEEKRLDEDRIFVSEVAIMEAEMGAGDENMEEAATWMEGEMLNGKRRYNHIRGDIWAARLMKDRELWLYRDKRIVGSIPGVYVGDVFLYMVELCVIGLHMQIQAGIDFLPKSRSPNGEPIATSVIVSSGYEDDMELDNGDVIIYTGQGGRKKNSSIQICDQKLEGGNLALERSMHYGIEIRVIRGMKYEGGASSSGKVYVYDGLYRIIDCWFEEGMNGFRVFKYKLLRVEGQTKMGSVVLKDARKIKKIGLDVKPMNCLSVDISHKRENVPIRLFNDIDDNEEPLNYEYLPKTIFPWFVFHQSGKAIGCECVEACTDGCFCSMKNGGEFPYNLQGLLVKGKPLIFECGPFCSCPPNCRNRVAQKGIKHRLEVFRSAQTGWGVRSLDLIQAGAFICEYTGVILTREQAQILTMNGDSLIYPKRFIDRWAEWGDLSQIYNNYVRPSYPLIPDIDYSMDISTLRNVASYLSHSSSPNVFVQFVLHDHNNLMFPRLMLYAMENIPPLRELVIDYGIPDEWAGKLSVCM