AlfalfaGEDB | MS.gene05656

M. sativa cultivar XinJiangDaYe / MS.gene05656

KEGG: K08065
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00808.26

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05656.t1	XP_004516113.2	44.5	238	120	2	1	238	6	231	2.90E-42	182.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05656.t1	O23310	46.4	84	45	0	56	139	26	109	2.2e-14	80.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05656.t1	A0A1S2Z6W5	44.5	238	120	2	1	238	6	231	2.1e-42	182.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene05656.t1	TF	NF-YB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05656.t1	MTR_5g095740	44.681	94	51	1	47	139	18	111	3.91e-22	90.1
MS.gene05656.t1	MTR_3g058980	45.000	100	52	1	56	155	32	128	9.58e-22	89.4
MS.gene05656.t1	MTR_1g083070	42.529	87	50	0	55	141	8	94	2.33e-21	86.7
MS.gene05656.t1	MTR_2g026710	41.000	100	58	1	41	139	6	105	3.06e-21	87.4
MS.gene05656.t1	MTR_7g100650	41.053	95	55	1	46	139	20	114	4.25e-21	87.0
MS.gene05656.t1	MTR_7g061270	41.053	95	55	1	46	139	20	114	4.67e-21	87.0
MS.gene05656.t1	MTR_1g039040	35.433	127	79	3	49	172	3	129	1.11e-20	86.7
MS.gene05656.t1	MTR_8g093920	46.429	84	45	0	56	139	45	128	1.37e-20	86.7
MS.gene05656.t1	MTR_8g091720	38.095	105	64	1	36	139	12	116	1.91e-20	86.3
MS.gene05656.t1	MTR_1g088860	42.857	84	48	0	56	139	41	124	3.56e-20	85.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05656.t1	AT2G38880	36.607	112	70	1	38	148	7	118	1.04e-22	90.5
MS.gene05656.t1	AT2G38880	37.864	103	63	1	38	139	7	109	2.96e-22	89.4
MS.gene05656.t1	AT2G38880	37.864	103	63	1	38	139	7	109	2.96e-22	89.4
MS.gene05656.t1	AT2G38880	37.864	103	63	1	38	139	7	109	2.96e-22	89.4
MS.gene05656.t1	AT2G38880	37.864	103	63	1	38	139	7	109	3.52e-22	89.0
MS.gene05656.t1	AT2G38880	37.864	103	63	1	38	139	7	109	6.21e-22	89.0
MS.gene05656.t1	AT2G38880	37.864	103	63	1	38	139	7	109	6.21e-22	89.0
MS.gene05656.t1	AT4G14540	46.429	84	45	0	56	139	26	109	8.43e-22	88.6
MS.gene05656.t1	AT2G38880	37.864	103	63	1	38	139	7	109	9.60e-22	87.0
MS.gene05656.t1	AT2G37060	41.935	93	53	1	48	139	26	118	7.26e-21	86.7

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Find 53 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATATTCCCTCGTTAACAA+TGG	0.602859	3.2:+51328523	MS.gene05656:CDS
AACGTTGTCACAGGGAATGC+AGG	0.505564	3.2:+51329052	MS.gene05656:CDS
AAGCCATTGAACGTTGTCAC+AGG	0.498720	3.2:+51329043	MS.gene05656:CDS
AAGCCTGACCCAATTGCAAT+GGG	0.536639	3.2:-51329270	None:intergenic
AAGTTTGTTAAAGCAGTCTC+TGG	0.385163	3.2:-51329384	None:intergenic
AATGGGTGGCGGTGGTGGTA+AGG	0.484109	3.2:-51329253	None:intergenic
AATTGCTAGTTGGGGTACAA+GGG	0.641551	3.2:-51328881	None:intergenic
AGAGCTGGTAAGGTGGGCTT+AGG	0.525041	3.2:-51329213	None:intergenic
AGCCATTGAACGTTGTCACA+GGG	0.613629	3.2:+51329044	MS.gene05656:CDS
AGCGATCTGAAGAGCTGGTA+AGG	0.528940	3.2:-51329223	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATATTCCCTCGTTAACAA+TGG	+	chr3.2:51328523-51328542	MS.gene05656:CDS	30.0%
AAGTATTTATTAGGATGTGC+AGG	+	chr3.2:51328781-51328800	MS.gene05656:intron	30.0%
ATTAAGGGTCATAAAGTCTA+TGG	+	chr3.2:51329098-51329117	MS.gene05656:CDS	30.0%
CTTGGATTCATTATACTTGT+TGG	-	chr3.2:51328853-51328872	None:intergenic	30.0%
TACCTTGCCAATAAAAAATG+TGG	+	chr3.2:51328915-51328934	MS.gene05656:CDS	30.0%
TAGACTTTATGACCCTTAAT+AGG	-	chr3.2:51329098-51329117	None:intergenic	30.0%
TCATCTATGTTGCATCAAAT+TGG	+	chr3.2:51328805-51328824	MS.gene05656:intron	30.0%
TGTCTCTAGATTTCAATACT+AGG	+	chr3.2:51328624-51328643	MS.gene05656:intron	30.0%
TTAAGGGTCATAAAGTCTAT+GGG	+	chr3.2:51329099-51329118	MS.gene05656:CDS	30.0%
TGACGATATCTTTCAAGAGA+TGG	+	chr3.2:51329431-51329450	MS.gene05656:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	51328514	51329473	51328514	ID=MS.gene05656
chr3.2	mRNA	51328514	51329473	51328514	ID=MS.gene05656.t1;Parent=MS.gene05656
chr3.2	exon	51328514	51328598	51328514	ID=MS.gene05656.t1.exon1;Parent=MS.gene05656.t1
chr3.2	CDS	51328514	51328598	51328514	ID=cds.MS.gene05656.t1;Parent=MS.gene05656.t1
chr3.2	exon	51328839	51329473	51328839	ID=MS.gene05656.t1.exon2;Parent=MS.gene05656.t1
chr3.2	CDS	51328839	51329473	51328839	ID=cds.MS.gene05656.t1;Parent=MS.gene05656.t1

Gene Sequence

>MS.gene05656

ATGGAGAATAAATATTCCCTCGTTAACAATGGTCAAAATCCAACTCAAGCTCTGGTAAAACACAAAATCAGAATAACTTACACACGTATATGAATTTTATTTAATAAAATTGTCTCTAGATTTCAATACTAGGTTGATAGATGAGTTGGTTTTGAGTCATTTCAAACCATAGATCAAATTGTTATTTTATTTTTAACTATTTGATTCATTTTGCTTTCTATCAGTGTTTTGCATACAATGTGTTATTTTTAAATGTTAAAATAATGAAAGTATTTATTAGGATGTGCAGGTTCATCTATGTTGCATCAAATTGGTGCCTCTGCAGATACAAGAGCTCCAACAAGTATAATGAATCCAAGAAATGATGCCCTTGTACCCCAACTAGCAATTCGAGACAAAAATACCTTGCCAATAAAAAATGTGGCTACAATCATGAGAAAAGTTCTTCCACCAAACACAAAAATCTCTCGTGGAGCAAAGGAGATGGTTGAACAAATCACCTCAAAGTTTATAAGATATGTAACCAAAAAAGCCATTGAACGTTGTCACAGGGAATGCAGGAAAATTTTGGGTGCAGATGACCTATTAAGGGTCATAAAGTCTATGGGTTATAATAACTATTATGAGTATCTCAATTTTTATCTCAAATGCTTTCGTTATAGTAATGAAATTGGATATTTGAAACTTGTTGGTGAGCTTCCTAAGCCCACCTTACCAGCTCTTCAGATCGCTTCTCCCACCTTACCACCACCGCCACCCATTGCAATTGGGTCAGGCTTATCTTTGAACTCAAACTCATTTATTGATTTGGATGAAGCAGCGGGCATGAATGAGGTTTGGAATGAATTGGATAATTTGGGTGCTGGTTCCCCAGAGACTGCTTTAACAAACTTTGATCATTCAATTCAATTCAACTTTGACGATATCTTTCAAGAGATGGATGATAAAATGAATGATTAA

Protein sequence

>MS.gene05656.t1

MENKYSLVNNGQNPTQALVKHKIRITYTHTRAPTSIMNPRNDALVPQLAIRDKNTLPIKNVATIMRKVLPPNTKISRGAKEMVEQITSKFIRYVTKKAIERCHRECRKILGADDLLRVIKSMGYNNYYEYLNFYLKCFRYSNEIGYLKLVGELPKPTLPALQIASPTLPPPPPIAIGSGLSLNSNSFIDLDEAAGMNEVWNELDNLGAGSPETALTNFDHSIQFNFDDIFQEMDDKMND