Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056747.t1 | XP_013444885.1 | 98.7 | 154 | 2 | 0 | 1 | 154 | 52 | 205 | 7.70E-81 | 309.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056747.t1 | Q1ECF5 | 64.4 | 149 | 53 | 0 | 1 | 149 | 52 | 200 | 1.9e-51 | 203.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056747.t1 | A0A072TZG0 | 98.7 | 154 | 2 | 0 | 1 | 154 | 52 | 205 | 5.5e-81 | 309.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene056747.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056747.t1 | MTR_8g031490 | 98.701 | 154 | 2 | 0 | 1 | 154 | 52 | 205 | 1.97e-112 | 317 |
| MS.gene056747.t1 | MTR_8g031490 | 53.659 | 82 | 37 | 1 | 46 | 126 | 3 | 84 | 1.33e-24 | 94.4 |
| MS.gene056747.t1 | MTR_7g076720 | 71.795 | 156 | 42 | 2 | 1 | 154 | 52 | 207 | 6.64e-74 | 220 |
| MS.gene056747.t1 | MTR_7g076720 | 57.746 | 71 | 30 | 0 | 46 | 116 | 3 | 73 | 3.76e-24 | 93.6 |
| MS.gene056747.t1 | MTR_3g070050 | 60.294 | 136 | 48 | 2 | 1 | 130 | 10 | 145 | 4.65e-57 | 175 |
| MS.gene056747.t1 | MTR_3g070050 | 60.294 | 136 | 48 | 2 | 1 | 130 | 52 | 187 | 3.14e-56 | 174 |
| MS.gene056747.t1 | MTR_3g070050 | 53.521 | 71 | 33 | 0 | 46 | 116 | 3 | 73 | 4.78e-22 | 87.4 |
| MS.gene056747.t1 | MTR_8g097300 | 53.947 | 152 | 64 | 3 | 1 | 151 | 52 | 198 | 2.92e-51 | 162 |
| MS.gene056747.t1 | MTR_8g097300 | 42.424 | 66 | 38 | 0 | 46 | 111 | 3 | 68 | 6.11e-15 | 69.3 |
| MS.gene056747.t1 | MTR_1g017950 | 45.139 | 144 | 72 | 3 | 1 | 138 | 52 | 194 | 1.74e-37 | 127 |
| MS.gene056747.t1 | MTR_1g017950 | 51.471 | 68 | 33 | 0 | 46 | 113 | 3 | 70 | 1.44e-18 | 78.6 |
| MS.gene056747.t1 | MTR_4g106800 | 44.961 | 129 | 58 | 2 | 1 | 120 | 53 | 177 | 1.36e-34 | 119 |
| MS.gene056747.t1 | MTR_4g106800 | 46.377 | 69 | 37 | 0 | 46 | 114 | 4 | 72 | 1.64e-16 | 72.8 |
| MS.gene056747.t1 | MTR_3g070033 | 56.923 | 65 | 28 | 0 | 66 | 130 | 6 | 70 | 1.27e-24 | 90.9 |
| MS.gene056747.t1 | MTR_1g106100 | 35.849 | 106 | 66 | 1 | 8 | 111 | 20 | 125 | 2.43e-18 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056747.t1 | AT3G61230 | 68.243 | 148 | 43 | 2 | 1 | 144 | 53 | 200 | 2.30e-72 | 216 |
| MS.gene056747.t1 | AT3G61230 | 45.833 | 96 | 48 | 1 | 46 | 141 | 4 | 95 | 2.38e-25 | 96.7 |
| MS.gene056747.t1 | AT2G45800 | 66.667 | 153 | 45 | 4 | 1 | 147 | 21 | 173 | 1.02e-68 | 206 |
| MS.gene056747.t1 | AT1G01780 | 64.430 | 149 | 53 | 0 | 1 | 149 | 52 | 200 | 3.53e-68 | 205 |
| MS.gene056747.t1 | AT1G01780 | 45.833 | 96 | 46 | 1 | 46 | 141 | 3 | 92 | 1.86e-24 | 94.0 |
| MS.gene056747.t1 | AT2G45800 | 66.667 | 153 | 45 | 4 | 1 | 147 | 52 | 204 | 4.25e-68 | 206 |
| MS.gene056747.t1 | AT2G45800 | 60.563 | 71 | 28 | 0 | 46 | 116 | 3 | 73 | 6.58e-26 | 98.6 |
| MS.gene056747.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene056747.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene056747.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene056747.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene056747.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene056747.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene056747.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene056747.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene056747.t1 | AT2G39900 | 66.142 | 127 | 38 | 2 | 1 | 122 | 52 | 178 | 3.64e-60 | 185 |
| MS.gene056747.t1 | AT2G39900 | 48.101 | 79 | 41 | 0 | 46 | 124 | 3 | 81 | 8.36e-21 | 84.7 |
| MS.gene056747.t1 | AT3G55770 | 53.125 | 160 | 37 | 3 | 1 | 122 | 52 | 211 | 8.10e-53 | 167 |
| MS.gene056747.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 5.51e-20 | 83.2 |
| MS.gene056747.t1 | AT1G10200 | 45.161 | 124 | 62 | 2 | 1 | 118 | 10 | 133 | 1.35e-35 | 120 |
| MS.gene056747.t1 | AT1G10200 | 45.161 | 124 | 62 | 2 | 1 | 118 | 52 | 175 | 1.25e-34 | 119 |
| MS.gene056747.t1 | AT1G10200 | 50.000 | 68 | 34 | 0 | 46 | 113 | 3 | 70 | 1.88e-17 | 75.5 |
| MS.gene056747.t1 | AT3G55770 | 64.634 | 82 | 24 | 3 | 1 | 78 | 52 | 132 | 2.09e-28 | 102 |
| MS.gene056747.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 3.90e-21 | 84.0 |
| MS.gene056747.t1 | AT3G55770 | 65.789 | 76 | 20 | 3 | 1 | 70 | 52 | 127 | 8.81e-26 | 95.5 |
| MS.gene056747.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.19e-21 | 84.3 |
Find 28 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATTCAAGAGGTTTCTTC+TGG | 0.235129 | 8.4:+65765276 | None:intergenic |
| GTTTCTTCTGGATGATCTTC+AGG | 0.252303 | 8.4:+65765288 | None:intergenic |
| TCAAGCTATGCTGCTCTTGA+TGG | 0.353414 | 8.4:-65765456 | MS.gene056747:CDS |
| CAAAATGAACCCTGCAGTAT+AGG | 0.377575 | 8.4:+65765430 | None:intergenic |
| AGCAAGTCGCACATTTGTCC+AGG | 0.436853 | 8.4:+65765865 | None:intergenic |
| TGCTTTAGGTGTGCTCATGC+AGG | 0.437095 | 8.4:-65765495 | MS.gene056747:CDS |
| TTGATGGTGTCCTATACTGC+AGG | 0.448405 | 8.4:-65765440 | MS.gene056747:CDS |
| TCTACCACAAGACATGCTTT+AGG | 0.453453 | 8.4:-65765509 | MS.gene056747:CDS |
| AACCGTCTATCCACTGGAAA+AGG | 0.456725 | 8.4:-65765838 | MS.gene056747:intron |
| CTCTAAAACCGTCTATCCAC+TGG | 0.500295 | 8.4:-65765844 | MS.gene056747:CDS |
| TTGTTACTGTTTCATTCAAG+AGG | 0.524503 | 8.4:+65765266 | None:intergenic |
| TTCCATGTTCAGTGGTACCC+TGG | 0.525905 | 8.4:-65765883 | MS.gene056747:CDS |
| AACATGGAAGATAGTCTACT+TGG | 0.545011 | 8.4:+65765897 | None:intergenic |
| GGTGCTTCTGCTGATTGTGA+AGG | 0.545791 | 8.4:+65765309 | None:intergenic |
| ATTAAACAGGTGACACTGGA+AGG | 0.549370 | 8.4:-65765537 | MS.gene056747:intron |
| GCAATAGAGAATTCCATCCA+TGG | 0.558350 | 8.4:+65766471 | None:intergenic |
| AGTACATACTCCTCCATGGA+TGG | 0.562913 | 8.4:-65766484 | None:intergenic |
| TTCTGACAAAGCAAATGAGC+TGG | 0.565336 | 8.4:-65766255 | MS.gene056747:intron |
| TACTCAAATCTGCACACAAG+AGG | 0.573033 | 8.4:-65765371 | MS.gene056747:CDS |
| GCTTTAGGTGTGCTCATGCA+GGG | 0.590942 | 8.4:-65765494 | MS.gene056747:CDS |
| TGATGGTGTCCTATACTGCA+GGG | 0.598936 | 8.4:-65765439 | MS.gene056747:CDS |
| GTCCAGGGTACCACTGAACA+TGG | 0.612761 | 8.4:+65765881 | None:intergenic |
| GCATAGCTTGAATGTGTGAG+AGG | 0.618734 | 8.4:+65765468 | None:intergenic |
| AAATCTGCACACAAGAGGAA+TGG | 0.618865 | 8.4:-65765366 | MS.gene056747:CDS |
| CATAGCTTGAATGTGTGAGA+GGG | 0.638300 | 8.4:+65765469 | None:intergenic |
| AGACTATCTTCCATGTTCAG+TGG | 0.654978 | 8.4:-65765891 | MS.gene056747:CDS |
| GCAAGTCGCACATTTGTCCA+GGG | 0.661497 | 8.4:+65765866 | None:intergenic |
| CACACCTAAAGCATGTCTTG+TGG | 0.697099 | 8.4:+65765505 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATCTCTAATAATTTA+TGG | + | chr8.4:65765664-65765683 | None:intergenic | 10.0% |
| !! | TAAAATAAAAATTCAAGTAA+TGG | + | chr8.4:65765564-65765583 | None:intergenic | 10.0% |
| !!! | AATTGTTTCTTTTAAAAATT+TGG | - | chr8.4:65765999-65766018 | MS.gene056747:intron | 10.0% |
| !!! | ATATAATTTTTTGTTCATTT+TGG | - | chr8.4:65765439-65765458 | MS.gene056747:CDS | 10.0% |
| !! | AAAATAAAAAATGAAGGATT+TGG | + | chr8.4:65765366-65765385 | None:intergenic | 15.0% |
| !! | AAAATTATATGTGTATCAAT+TGG | + | chr8.4:65765429-65765448 | None:intergenic | 15.0% |
| !! | TAAAAGATAAAGATAAGATA+TGG | + | chr8.4:65765527-65765546 | None:intergenic | 15.0% |
| !! | AATATGGAATTCATAAATGA+AGG | - | chr8.4:65766138-65766157 | MS.gene056747:intron | 20.0% |
| !!! | AGACAAATTTCATATCTTTT+TGG | + | chr8.4:65765632-65765651 | None:intergenic | 20.0% |
| !!! | GCTTTTTTAAATCAAATTCT+TGG | + | chr8.4:65766052-65766071 | None:intergenic | 20.0% |
| !!! | TTTTTCTGGTTTTATTAAAC+AGG | - | chr8.4:65766200-65766219 | MS.gene056747:intron | 20.0% |
| ! | AAAAAGTTGAATTAGCCATA+TGG | - | chr8.4:65765596-65765615 | MS.gene056747:intron | 25.0% |
| ! | AATTAGCCATATGGAAATTA+GGG | - | chr8.4:65765605-65765624 | MS.gene056747:intron | 25.0% |
| ! | TTACCTGATTTCATATAACT+TGG | - | chr8.4:65766025-65766044 | MS.gene056747:intron | 25.0% |
| ! | TTTAAACACAAGCTGTAATA+TGG | - | chr8.4:65766122-65766141 | MS.gene056747:intron | 25.0% |
| !! | AACAATTTTCCACAAAATCA+AGG | + | chr8.4:65765986-65766005 | None:intergenic | 25.0% |
| !! | TCTTTCTAGTAGTGATATAA+AGG | - | chr8.4:65765725-65765744 | MS.gene056747:intron | 25.0% |
| !!! | GTATTGATCTTTTACAGAAT+AGG | - | chr8.4:65765827-65765846 | MS.gene056747:intron | 25.0% |
| GAATTAGCCATATGGAAATT+AGG | - | chr8.4:65765604-65765623 | MS.gene056747:intron | 30.0% | |
| ! | AAAGTCGTAACACATTTTTC+TGG | - | chr8.4:65766186-65766205 | MS.gene056747:intron | 30.0% |
| ! | ACATTTTGAACAGCTTTTCA+AGG | - | chr8.4:65765307-65765326 | MS.gene056747:CDS | 30.0% |
| ! | GAAAAATGTGTTACGACTTT+TGG | + | chr8.4:65766186-65766205 | None:intergenic | 30.0% |
| ! | TCATTTTGCACAACTTTTCA+TGG | - | chr8.4:65766335-65766354 | MS.gene056747:intron | 30.0% |
| ! | TTTTATTAAACAGGTGACAC+TGG | - | chr8.4:65766209-65766228 | MS.gene056747:intron | 30.0% |
| AAAGCAATCATACGTTTCGT+TGG | - | chr8.4:65766067-65766086 | MS.gene056747:intron | 35.0% | |
| AACATGGAAGATAGTCTACT+TGG | + | chr8.4:65765856-65765875 | None:intergenic | 35.0% | |
| ATCATACGTTTCGTTGGATA+TGG | - | chr8.4:65766073-65766092 | MS.gene056747:intron | 35.0% | |
| GGTCCAAGTTATATGAAATC+AGG | + | chr8.4:65766031-65766050 | None:intergenic | 35.0% | |
| TTTGGTCCCTAATTTCCATA+TGG | + | chr8.4:65765614-65765633 | None:intergenic | 35.0% | |
| ! | TGTTGTGATCCTTGATTTTG+TGG | - | chr8.4:65765974-65765993 | MS.gene056747:intron | 35.0% |
| !! | GGCAATTTTAGCAAGAACTT+TGG | - | chr8.4:65765335-65765354 | MS.gene056747:CDS | 35.0% |
| AAATCTGCACACAAGAGGAA+TGG | - | chr8.4:65766384-65766403 | MS.gene056747:intron | 40.0% | |
| AGACTATCTTCCATGTTCAG+TGG | - | chr8.4:65765859-65765878 | MS.gene056747:CDS | 40.0% | |
| ATTAAACAGGTGACACTGGA+AGG | - | chr8.4:65766213-65766232 | MS.gene056747:intron | 40.0% | |
| CAAAATGAACCCTGCAGTAT+AGG | + | chr8.4:65766323-65766342 | None:intergenic | 40.0% | |
| CATAGCTTGAATGTGTGAGA+GGG | + | chr8.4:65766284-65766303 | None:intergenic | 40.0% | |
| GTTTCTTCTGGATGATCTTC+AGG | + | chr8.4:65766465-65766484 | None:intergenic | 40.0% | |
| TACTCAAATCTGCACACAAG+AGG | - | chr8.4:65766379-65766398 | MS.gene056747:intron | 40.0% | |
| TCTACCACAAGACATGCTTT+AGG | - | chr8.4:65766241-65766260 | MS.gene056747:intron | 40.0% | |
| TTCTGACAAAGCAAATGAGC+TGG | - | chr8.4:65765495-65765514 | MS.gene056747:CDS | 40.0% | |
| ! | AACCTTTTCCAGTGGATAGA+CGG | + | chr8.4:65765917-65765936 | None:intergenic | 40.0% |
| ! | GCACAACTTTTCATGGAGAA+AGG | - | chr8.4:65766342-65766361 | MS.gene056747:intron | 40.0% |
| ! | TGTCGCGATTCTTGATTTTG+AGG | - | chr8.4:65765937-65765956 | MS.gene056747:intron | 40.0% |
| ! | TGTGTTACGACTTTTGGTCA+CGG | + | chr8.4:65766180-65766199 | None:intergenic | 40.0% |
| !! | GAACAGCTTTTCAAGGAATC+TGG | - | chr8.4:65765314-65765333 | MS.gene056747:CDS | 40.0% |
| AACCGTCTATCCACTGGAAA+AGG | - | chr8.4:65765912-65765931 | MS.gene056747:intron | 45.0% | |
| CACACCTAAAGCATGTCTTG+TGG | + | chr8.4:65766248-65766267 | None:intergenic | 45.0% | |
| GCATAGCTTGAATGTGTGAG+AGG | + | chr8.4:65766285-65766304 | None:intergenic | 45.0% | |
| TCAAGCTATGCTGCTCTTGA+TGG | - | chr8.4:65766294-65766313 | MS.gene056747:intron | 45.0% | |
| ! | TGATGGTGTCCTATACTGCA+GGG | - | chr8.4:65766311-65766330 | MS.gene056747:intron | 45.0% |
| !! | CTCTAAAACCGTCTATCCAC+TGG | - | chr8.4:65765906-65765925 | MS.gene056747:CDS | 45.0% |
| !! | TTGATGGTGTCCTATACTGC+AGG | - | chr8.4:65766310-65766329 | MS.gene056747:intron | 45.0% |
| !! | AAAATTAAAATAAAAAATGA+AGG | + | chr8.4:65765372-65765391 | None:intergenic | 5.0% |
| AGCAAGTCGCACATTTGTCC+AGG | + | chr8.4:65765888-65765907 | None:intergenic | 50.0% | |
| GCAAGTCGCACATTTGTCCA+GGG | + | chr8.4:65765887-65765906 | None:intergenic | 50.0% | |
| TTCCATGTTCAGTGGTACCC+TGG | - | chr8.4:65765867-65765886 | MS.gene056747:CDS | 50.0% | |
| ! | CGACTTTTGGTCACGGAGTT+TGG | + | chr8.4:65766173-65766192 | None:intergenic | 50.0% |
| ! | GCTTTAGGTGTGCTCATGCA+GGG | - | chr8.4:65766256-65766275 | MS.gene056747:CDS | 50.0% |
| ! | GGTGCTTCTGCTGATTGTGA+AGG | + | chr8.4:65766444-65766463 | None:intergenic | 50.0% |
| ! | TCGCGACAAACCTTTTCCAG+TGG | + | chr8.4:65765925-65765944 | None:intergenic | 50.0% |
| ! | TGCTTTAGGTGTGCTCATGC+AGG | - | chr8.4:65766255-65766274 | MS.gene056747:intron | 50.0% |
| ! | GTCCAGGGTACCACTGAACA+TGG | + | chr8.4:65765872-65765891 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 65765281 | 65766491 | 65765281 | ID=MS.gene056747 |
| chr8.4 | mRNA | 65765281 | 65766491 | 65765281 | ID=MS.gene056747.t1;Parent=MS.gene056747 |
| chr8.4 | exon | 65766416 | 65766491 | 65766416 | ID=MS.gene056747.t1.exon1;Parent=MS.gene056747.t1 |
| chr8.4 | CDS | 65766416 | 65766491 | 65766416 | ID=cds.MS.gene056747.t1;Parent=MS.gene056747.t1 |
| chr8.4 | exon | 65766256 | 65766284 | 65766256 | ID=MS.gene056747.t1.exon2;Parent=MS.gene056747.t1 |
| chr8.4 | CDS | 65766256 | 65766284 | 65766256 | ID=cds.MS.gene056747.t1;Parent=MS.gene056747.t1 |
| chr8.4 | exon | 65765839 | 65765928 | 65765839 | ID=MS.gene056747.t1.exon3;Parent=MS.gene056747.t1 |
| chr8.4 | CDS | 65765839 | 65765928 | 65765839 | ID=cds.MS.gene056747.t1;Parent=MS.gene056747.t1 |
| chr8.4 | exon | 65765281 | 65765550 | 65765281 | ID=MS.gene056747.t1.exon4;Parent=MS.gene056747.t1 |
| chr8.4 | CDS | 65765281 | 65765550 | 65765281 | ID=cds.MS.gene056747.t1;Parent=MS.gene056747.t1 |
| Gene Sequence |
| Protein sequence |
