AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056847

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056847.t1 MTR_1g060700 94.569 313 13 3 1 312 178 487 0.0 598
MS.gene056847.t1 MTR_2g067000 81.366 322 26 10 1 312 1 298 5.33e-163 456
MS.gene056847.t1 MTR_8g093500 58.491 53 22 0 182 234 125 177 2.85e-13 69.3
MS.gene056847.t1 MTR_3g101810 58.491 53 22 0 182 234 182 234 1.78e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056847.t1 AT1G30670 41.520 171 97 2 135 305 95 262 2.82e-29 113
MS.gene056847.t1 AT2G34820 42.254 213 115 4 97 305 81 289 2.29e-28 111
MS.gene056847.t1 AT3G21330 60.377 53 21 0 182 234 280 332 2.02e-13 70.5
MS.gene056847.t1 AT5G01305 50.000 58 29 0 182 239 48 105 1.23e-12 65.5
MS.gene056847.t1 AT4G00120 52.830 53 25 0 182 234 123 175 6.45e-12 63.9

Find 56 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGGAATGCTTCAACTTA+TGG 0.195386 1.1:-46148666 MS.gene056847:CDS
ATCTCTGCTGTGTTCATCTT+TGG 0.238213 1.1:+46148731 None:intergenic
TTGGTGAAATCTTCCTCATT+TGG 0.290806 1.1:+46149052 None:intergenic
ATCAAAACCTACCATTCTTA+AGG 0.300143 1.1:-46147638 MS.gene056847:CDS
TCAAATAGTTAGAGGGAAAA+TGG 0.318116 1.1:+46149227 None:intergenic
AAATAAAACTATTCTTGCTT+TGG 0.325467 1.1:+46147577 None:intergenic
TGGTGAAATCTTCCTCATTT+GGG 0.332786 1.1:+46149053 None:intergenic
GAAGCAAAAGGGTTAGGGTT+AGG 0.345524 1.1:+46148917 None:intergenic
AAGCAAAAGGGTTAGGGTTA+GGG 0.345934 1.1:+46148918 None:intergenic
TCTCCCCGTAGCATAGCTTT+TGG 0.368098 1.1:+46149008 None:intergenic
AGTTGATCTTGACAAGGGTT+AGG 0.369040 1.1:+46149136 None:intergenic
TCAAAGGAAGCAAAAGGGTT+AGG 0.369513 1.1:+46148911 None:intergenic
AGTAGTGATTTCTAAGTTGT+TGG 0.372241 1.1:+46149291 None:intergenic
GTAGTTTGAATAGGTTGATA+AGG 0.378786 1.1:+46149313 None:intergenic
TACAGTTTCTCTTGAACAAA+TGG 0.383908 1.1:+46147727 None:intergenic
AAGGGTCTGAAACAGCTTGC+TGG 0.388228 1.1:-46147619 MS.gene056847:CDS
AATGTGGTGACAAACTCTTT+TGG 0.390869 1.1:+46147682 None:intergenic
GAGCTTGGGAAGTTGGTTCC+TGG 0.392352 1.1:-46148758 MS.gene056847:CDS
GACACAAGAGCTTGGGAAGT+TGG 0.397999 1.1:-46148765 MS.gene056847:CDS
AAGTTGTTGGTAGTTTGAAT+AGG 0.402079 1.1:+46149304 None:intergenic
TTGATTATTTCAGGAAGAAA+AGG 0.406675 1.1:-46147791 MS.gene056847:intron
TAGTTTGAATAGGTTGATAA+GGG 0.429054 1.1:+46149314 None:intergenic
AGTTTCATTCAAATAGTTAG+AGG 0.435549 1.1:+46149219 None:intergenic
GTTGATCTTGACAAGGGTTA+GGG 0.453473 1.1:+46149137 None:intergenic
TCAAAACCTACCATTCTTAA+GGG 0.456430 1.1:-46147637 MS.gene056847:CDS
CAAAGGAAGCAAAAGGGTTA+GGG 0.461232 1.1:+46148912 None:intergenic
AGAGTATCGCGGCGAGAGAA+AGG 0.466876 1.1:-46148808 MS.gene056847:CDS
TCTACCTCAAAGGAAGCAAA+AGG 0.468900 1.1:+46148905 None:intergenic
TTGTGTAGATCTTCACTTGG+AGG 0.473071 1.1:+46147763 None:intergenic
GTTCAATGTGAGAAGAAAGT+TGG 0.488552 1.1:-46148851 MS.gene056847:CDS
TGTAGATCTTCACTTGGAGG+AGG 0.494574 1.1:+46147766 None:intergenic
GTGTTCATCTTTGGTCCACC+AGG 0.499817 1.1:+46148740 None:intergenic
ACTGAGAAGACACAAGAGCT+TGG 0.499993 1.1:-46148773 MS.gene056847:CDS
CTGAGAAGACACAAGAGCTT+GGG 0.500383 1.1:-46148772 MS.gene056847:CDS
AACGCCAAAAGCTATGCTAC+GGG 0.513263 1.1:-46149012 MS.gene056847:CDS
CTGTTTCAGACCCTTAAGAA+TGG 0.516519 1.1:+46147627 None:intergenic
AGGGTCTGAAACAGCTTGCT+GGG 0.522554 1.1:-46147618 MS.gene056847:CDS
AAACGCCAAAAGCTATGCTA+CGG 0.538423 1.1:-46149013 MS.gene056847:CDS
GTTTCATTCAAATAGTTAGA+GGG 0.549802 1.1:+46149220 None:intergenic
CTTGGGAAGTTGGTTCCTGG+TGG 0.551828 1.1:-46148755 MS.gene056847:CDS
GTGAGAAGAAAGTTGGTGAA+AGG 0.566992 1.1:-46148844 MS.gene056847:CDS
AAGTTTCTTCAAGCTCAAGT+TGG 0.567049 1.1:-46148683 MS.gene056847:CDS
AGCAAAGGGTTCTACCTCAA+AGG 0.572532 1.1:+46148895 None:intergenic
AGTTTGTGTAGATCTTCACT+TGG 0.582386 1.1:+46147760 None:intergenic
TTCAGACCCTTAAGAATGGT+AGG 0.583734 1.1:+46147631 None:intergenic
GAGTTCAAAAGCTCTTGTTG+TGG 0.586358 1.1:+46148974 None:intergenic
TCGCGGCGAGAGAAAGGAGA+AGG 0.587504 1.1:-46148802 MS.gene056847:CDS
ACTTATGGAAACATTTAACA+AGG 0.595324 1.1:-46148651 MS.gene056847:intron
CTACCTCAAAGGAAGCAAAA+GGG 0.620756 1.1:+46148906 None:intergenic
AATAGTTAGAGGGAAAATGG+TGG 0.625963 1.1:+46149230 None:intergenic
ACAAGTAGTTGATCTTGACA+AGG 0.633389 1.1:+46149130 None:intergenic
CAAGTAGTTGATCTTGACAA+GGG 0.656926 1.1:+46149131 None:intergenic
ATCATTATGATTGCTCAATG+TGG 0.666694 1.1:+46147666 None:intergenic
ATGCTACGGGGAGAAAAGAG+AGG 0.672871 1.1:-46148999 MS.gene056847:CDS
TATCTCGTCGCAGAGTATCG+CGG 0.686396 1.1:-46148819 MS.gene056847:CDS
ACGCCAAAAGCTATGCTACG+GGG 0.725950 1.1:-46149011 MS.gene056847:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTTGTAAATATCTAAA+TGG - chr1.1:46148662-46148681 MS.gene056847:CDS 15.0%
!! CATAATAACACTACAATAAA+TGG + chr1.1:46148637-46148656 None:intergenic 20.0%
!! CGTTTGATAAAAAAATATAG+AGG - chr1.1:46148606-46148625 MS.gene056847:intron 20.0%
!! GGAATTGTTATAAAAATTGA+CGG - chr1.1:46148818-46148837 MS.gene056847:CDS 20.0%
!! TTTACTCCATGAAAATATTT+AGG - chr1.1:46148797-46148816 MS.gene056847:CDS 20.0%
!!! ACAATTCCTAAATATTTTCA+TGG + chr1.1:46148806-46148825 None:intergenic 20.0%
!!! TTTTGCTAATTGATTATTTC+AGG - chr1.1:46149137-46149156 MS.gene056847:CDS 20.0%
! AAACTCATCCACAAAAAAAT+TGG + chr1.1:46147991-46148010 None:intergenic 25.0%
! AAAGACAAAATTGAGACAAT+AGG + chr1.1:46148750-46148769 None:intergenic 25.0%
! ACTTATGGAAACATTTAACA+AGG - chr1.1:46148286-46148305 MS.gene056847:intron 25.0%
! AGAAAAACATCAAATTCACT+TGG + chr1.1:46148438-46148457 None:intergenic 25.0%
! AGTTTCATTCAAATAGTTAG+AGG + chr1.1:46147721-46147740 None:intergenic 25.0%
! GTTTCATTCAAATAGTTAGA+GGG + chr1.1:46147720-46147739 None:intergenic 25.0%
! GTTTCATTTGAGTAAAAACA+AGG + chr1.1:46147777-46147796 None:intergenic 25.0%
! TGTTAGGTTTAAGAAAATGT+TGG - chr1.1:46148848-46148867 MS.gene056847:CDS 25.0%
! TTGATTATTTCAGGAAGAAA+AGG - chr1.1:46149146-46149165 MS.gene056847:CDS 25.0%
! TTGTTATAAAAATTGACGGA+TGG - chr1.1:46148822-46148841 MS.gene056847:CDS 25.0%
! TTGTTTGTTCATTGCATATT+TGG - chr1.1:46148538-46148557 MS.gene056847:intron 25.0%
!! TATTTTCATGGAGTAAAAGT+TGG + chr1.1:46148794-46148813 None:intergenic 25.0%
!! TTAAACTGTTTTGTGTACTT+AGG - chr1.1:46148398-46148417 MS.gene056847:intron 25.0%
!!! ACTTTTTCTTCTCAAAATGA+TGG - chr1.1:46147837-46147856 MS.gene056847:intron 25.0%
!!! ATCATTTTGAGAAGAAAAAG+TGG + chr1.1:46147838-46147857 None:intergenic 25.0%
!!! TAGTTTGAATAGGTTGATAA+GGG + chr1.1:46147626-46147645 None:intergenic 25.0%
ACTCAAATGATTACAAGCAA+GGG + chr1.1:46148580-46148599 None:intergenic 30.0%
AGTAGTGATTTCTAAGTTGT+TGG + chr1.1:46147649-46147668 None:intergenic 30.0%
ATCAAAACCTACCATTCTTA+AGG - chr1.1:46149299-46149318 MS.gene056847:CDS 30.0%
ATCATTATGATTGCTCAATG+TGG + chr1.1:46149274-46149293 None:intergenic 30.0%
TAATGCTGTTGTTGTAGATT+TGG - chr1.1:46148689-46148708 MS.gene056847:CDS 30.0%
TACAGTTTCTCTTGAACAAA+TGG + chr1.1:46149213-46149232 None:intergenic 30.0%
TACTCAAATGATTACAAGCA+AGG + chr1.1:46148581-46148600 None:intergenic 30.0%
TCAAAACCTACCATTCTTAA+GGG - chr1.1:46149300-46149319 MS.gene056847:CDS 30.0%
TCAAATAGTTAGAGGGAAAA+TGG + chr1.1:46147713-46147732 None:intergenic 30.0%
! GAATGAAATTTTCCCAAATG+AGG - chr1.1:46147872-46147891 MS.gene056847:intron 30.0%
! TTTTGGACATGTAAGGTAAT+TGG + chr1.1:46147907-46147926 None:intergenic 30.0%
!! AAGTTGTTGGTAGTTTGAAT+AGG + chr1.1:46147636-46147655 None:intergenic 30.0%
!! CAATAAACGAGAGAGTATTT+AGG - chr1.1:46148950-46148969 MS.gene056847:CDS 30.0%
!! GTAGTTTGAATAGGTTGATA+AGG + chr1.1:46147627-46147646 None:intergenic 30.0%
!!! AACAATTTTTGAAGCAGCAA+AGG + chr1.1:46148060-46148079 None:intergenic 30.0%
!!! ACAATTTTTGAAGCAGCAAA+GGG + chr1.1:46148059-46148078 None:intergenic 30.0%
!!! GAACTCAACCAATTTTTTTG+TGG - chr1.1:46147980-46147999 MS.gene056847:intron 30.0%
AAGTTTCTTCAAGCTCAAGT+TGG - chr1.1:46148254-46148273 MS.gene056847:intron 35.0%
AATAGTTAGAGGGAAAATGG+TGG + chr1.1:46147710-46147729 None:intergenic 35.0%
ACAAGTAGTTGATCTTGACA+AGG + chr1.1:46147810-46147829 None:intergenic 35.0%
AGGAAACGGTTAATAAGAAG+AGG - chr1.1:46148920-46148939 MS.gene056847:CDS 35.0%
AGTTGGAATGCTTCAACTTA+TGG - chr1.1:46148271-46148290 MS.gene056847:intron 35.0%
AGTTTGTGTAGATCTTCACT+TGG + chr1.1:46149180-46149199 None:intergenic 35.0%
ATTGAGACAATAGGTAGTCA+TGG + chr1.1:46148741-46148760 None:intergenic 35.0%
CAAGTAGTTGATCTTGACAA+GGG + chr1.1:46147809-46147828 None:intergenic 35.0%
CAAGTGTATATCAAATGAGC+TGG - chr1.1:46148978-46148997 MS.gene056847:CDS 35.0%
CGGTTAATAAGAAGAGGAAA+TGG - chr1.1:46148926-46148945 MS.gene056847:CDS 35.0%
GCAATGAACAAACAAACTCT+AGG + chr1.1:46148533-46148552 None:intergenic 35.0%
GGCTATGTAACTGTTTAACA+AGG - chr1.1:46148999-46149018 MS.gene056847:CDS 35.0%
GTTCAATGTGAGAAGAAAGT+TGG - chr1.1:46148086-46148105 MS.gene056847:intron 35.0%
TGGTGAAATCTTCCTCATTT+GGG + chr1.1:46147887-46147906 None:intergenic 35.0%
TTGGTGAAATCTTCCTCATT+TGG + chr1.1:46147888-46147907 None:intergenic 35.0%
! AATGTGGTGACAAACTCTTT+TGG + chr1.1:46149258-46149277 None:intergenic 35.0%
! AATTGACGGATGGAATTGTT+AGG - chr1.1:46148832-46148851 MS.gene056847:CDS 35.0%
AAACGCCAAAAGCTATGCTA+CGG - chr1.1:46147924-46147943 MS.gene056847:intron 40.0%
AAGCAAAAGGGTTAGGGTTA+GGG + chr1.1:46148022-46148041 None:intergenic 40.0%
AGTTGATCTTGACAAGGGTT+AGG + chr1.1:46147804-46147823 None:intergenic 40.0%
ATCTCTGCTGTGTTCATCTT+TGG + chr1.1:46148209-46148228 None:intergenic 40.0%
CAAAGGAAGCAAAAGGGTTA+GGG + chr1.1:46148028-46148047 None:intergenic 40.0%
CTACCTCAAAGGAAGCAAAA+GGG + chr1.1:46148034-46148053 None:intergenic 40.0%
CTGTTTCAGACCCTTAAGAA+TGG + chr1.1:46149313-46149332 None:intergenic 40.0%
GAGAGAGAGAGTTAAGGAAA+CGG - chr1.1:46148906-46148925 MS.gene056847:CDS 40.0%
GAGTTCAAAAGCTCTTGTTG+TGG + chr1.1:46147966-46147985 None:intergenic 40.0%
GTTGATCTTGACAAGGGTTA+GGG + chr1.1:46147803-46147822 None:intergenic 40.0%
TCAAAGGAAGCAAAAGGGTT+AGG + chr1.1:46148029-46148048 None:intergenic 40.0%
TCTACCTCAAAGGAAGCAAA+AGG + chr1.1:46148035-46148054 None:intergenic 40.0%
TTCAGACCCTTAAGAATGGT+AGG + chr1.1:46149309-46149328 None:intergenic 40.0%
TTGTGTAGATCTTCACTTGG+AGG + chr1.1:46149177-46149196 None:intergenic 40.0%
! TAACCCTTTTGCTTCCTTTG+AGG - chr1.1:46148028-46148047 MS.gene056847:intron 40.0%
! TAGCATAGCTTTTGGCGTTT+TGG + chr1.1:46147924-46147943 None:intergenic 40.0%
!! GTGAGAAGAAAGTTGGTGAA+AGG - chr1.1:46148093-46148112 MS.gene056847:intron 40.0%
!! TTTGGCGTTTTGGACATGTA+AGG + chr1.1:46147914-46147933 None:intergenic 40.0%
AACGCCAAAAGCTATGCTAC+GGG - chr1.1:46147925-46147944 MS.gene056847:intron 45.0%
ACTGAGAAGACACAAGAGCT+TGG - chr1.1:46148164-46148183 MS.gene056847:intron 45.0%
AGCAAAGGGTTCTACCTCAA+AGG + chr1.1:46148045-46148064 None:intergenic 45.0%
CAGAGAGAGAGAGAGAGTTA+AGG - chr1.1:46148900-46148919 MS.gene056847:CDS 45.0%
CTGAGAAGACACAAGAGCTT+GGG - chr1.1:46148165-46148184 MS.gene056847:intron 45.0%
GAAGCAAAAGGGTTAGGGTT+AGG + chr1.1:46148023-46148042 None:intergenic 45.0%
TGTAGATCTTCACTTGGAGG+AGG + chr1.1:46149174-46149193 None:intergenic 45.0%
ACGCCAAAAGCTATGCTACG+GGG - chr1.1:46147926-46147945 MS.gene056847:intron 50.0%
ATGCTACGGGGAGAAAAGAG+AGG - chr1.1:46147938-46147957 MS.gene056847:intron 50.0%
GACACAAGAGCTTGGGAAGT+TGG - chr1.1:46148172-46148191 MS.gene056847:intron 50.0%
GTGTTCATCTTTGGTCCACC+AGG + chr1.1:46148200-46148219 None:intergenic 50.0%
! AAGGGTCTGAAACAGCTTGC+TGG - chr1.1:46149318-46149337 MS.gene056847:CDS 50.0%
! AGGGTCTGAAACAGCTTGCT+GGG - chr1.1:46149319-46149338 MS.gene056847:CDS 50.0%
! TCTCCCCGTAGCATAGCTTT+TGG + chr1.1:46147932-46147951 None:intergenic 50.0%
!! TATCTCGTCGCAGAGTATCG+CGG - chr1.1:46148118-46148137 MS.gene056847:intron 50.0%
! CTTGGGAAGTTGGTTCCTGG+TGG - chr1.1:46148182-46148201 MS.gene056847:intron 55.0%
! GAGCTTGGGAAGTTGGTTCC+TGG - chr1.1:46148179-46148198 MS.gene056847:intron 55.0%
!! AGAGTATCGCGGCGAGAGAA+AGG - chr1.1:46148129-46148148 MS.gene056847:intron 55.0%
TCGCGGCGAGAGAAAGGAGA+AGG - chr1.1:46148135-46148154 MS.gene056847:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 46147585 46149374 46147585 ID=MS.gene056847
chr1.1 mRNA 46147585 46149374 46147585 ID=MS.gene056847.t1;Parent=MS.gene056847
chr1.1 exon 46148652 46149374 46148652 ID=MS.gene056847.t1.exon1;Parent=MS.gene056847.t1
chr1.1 CDS 46148652 46149374 46148652 ID=cds.MS.gene056847.t1;Parent=MS.gene056847.t1
chr1.1 exon 46147585 46147800 46147585 ID=MS.gene056847.t1.exon2;Parent=MS.gene056847.t1
chr1.1 CDS 46147585 46147800 46147585 ID=cds.MS.gene056847.t1;Parent=MS.gene056847.t1
Gene Sequence

>MS.gene056847

ATGGGAGCACTTAGCACATACTTCAACTATAATTGTTACCCTTATCAACCTATTCAAACTACCAACAACTTAGAAATCACTACTTTTCAACATCATGAACAAGAACAATTTGTGCAACAAAACCACCATTTTCCCTCTAACTATTTGAATGAAACTTCTCTTTTAGATGAAAGCTTCATTTTTCAAACTCCTTGTTTTTACTCAAATGAAACTTACCCTAACCCTTGTCAAGATCAACTACTTGTTGACTCCACTTTTTCTTCTCAAAATGATGGCTTTGTTTCAATGAATGAAATTTTCCCAAATGAGGAAGATTTCACCAATTACCTTACATGTCCAAAACGCCAAAAGCTATGCTACGGGGAGAAAAGAGAGGAACCACAACAAGAGCTTTTGAACTCAACCAATTTTTTTGTGGATGAGTTTATGACTAACCCTAACCCTAACCCTTTTGCTTCCTTTGAGGTAGAACCCTTTGCTGCTTCAAAAATTGTTGATGTTGTTCAATGTGAGAAGAAAGTTGGTGAAAGGACTATCTCGTCGCAGAGTATCGCGGCGAGAGAAAGGAGAAGGAAGATAACTGAGAAGACACAAGAGCTTGGGAAGTTGGTTCCTGGTGGACCAAAGATGAACACAGCAGAGATGCTTAATGCTGCTGCTAATTATGTCAAGTTTCTTCAAGCTCAAGTTGGAATGCTTCAACTTATGGAAACATTTAACAAGGTAACTGTTTTTGTTTTTATTGTTGCAATGTTCTTATATTATTAGAGCTCATAATTGAAGTTAATTACTTGTTTTCACTTGAGTTTCAATTTAAACTGTTTTGTGTACTTAGGTCTTATTCAATACTCCAAGTGAATTTGATGTTTTTCTTTTGAATGATGTTTTTTGCTTTTTATATGTTATGCTATGCTACTTGATTTAGTTTTTGAGTTTATAATAATACCTAGAGTTTGTTTGTTCATTGCATATTTGGTTACTTTGTGTGAAGACCCTTGCTTGTAATCATTTGAGTACATGTCGTTTGATAAAAAAATATAGAGGATATGCCATTTATTGTAGTGTTATTATGTCATGTATTGTTGTAAATATCTAAATGGATATTAATGCTGTTGTTGTAGATTTGGTTAGTTTTATCATTTATGTATGTAATCCATGACTACCTATTGTCTCAATTTTGTCTTTTTTATATTGATTTTTTTCATACCAACTTTTACTCCATGAAAATATTTAGGAATTGTTATAAAAATTGACGGATGGAATTGTTAGGTTTAAGAAAATGTTGGTTACAATGTATGAGAGAGAGAGAGAGAGACAGAGAGAGAGAGAGAGTTAAGGAAACGGTTAATAAGAAGAGGAAATGGTCAATAAACGAGAGAGTATTTAGGAACAGCAAGTGTATATCAAATGAGCTGGCTATGTAACTGTTTAACAAGGAATTTTGCAATATTTTTTTAAATTTAAATATTCACTATTTAGTGCAATATTTTTGTAAAAGCTAAATTTCTAGTGTAACTTATATAATAAAAAGAATTCACGTATATATTTTAGATTTTGCTAATTGATTATTTCAGGAAGAAAAGGAACCTCCTCCAAGTGAAGATCTACACAAACTTCTTCTTTCTCCATTTGTTCAAGAGAAACTGTATTCAGAAGAAAAGTGCTTTGTTCCAAAAGAGTTTGTCACCACATTGAGCAATCATAATGATGTTCAATCAAAACCTACCATTCTTAAGGGTCTGAAACAGCTTGCTGGGACTGAGATTGAGAAGAAACCAAAGCAAGAATAG
Protein sequence

>MS.gene056847.t1

MGALSTYFNYNCYPYQPIQTTNNLEITTFQHHEQEQFVQQNHHFPSNYLNETSLLDESFIFQTPCFYSNETYPNPCQDQLLVDSTFSSQNDGFVSMNEIFPNEEDFTNYLTCPKRQKLCYGEKREEPQQELLNSTNFFVDEFMTNPNPNPFASFEVEPFAASKIVDVVQCEKKVGERTISSQSIAARERRRKITEKTQELGKLVPGGPKMNTAEMLNAAANYVKFLQAQVGMLQLMETFNKEEKEPPPSEDLHKLLLSPFVQEKLYSEEKCFVPKEFVTTLSNHNDVQSKPTILKGLKQLAGTEIEKKPKQE