AlfalfaGEDB | MS.gene056847

M. sativa cultivar XinJiangDaYe / MS.gene056847

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056847.t1	KEH42071.1	92.7	313	19	3	1	312	178	487	1.20E-157	565.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056847.t1	Q84RD0	40.1	247	133	6	61	300	46	284	3.1e-29	130.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056847.t1	A0A072VKB3	92.7	313	19	3	1	312	178	487	8.7e-158	565.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene056847.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056847.t1	MTR_1g060700	94.569	313	13	3	1	312	178	487	0.0	598
MS.gene056847.t1	MTR_2g067000	81.366	322	26	10	1	312	1	298	5.33e-163	456
MS.gene056847.t1	MTR_8g093500	58.491	53	22	0	182	234	125	177	2.85e-13	69.3
MS.gene056847.t1	MTR_3g101810	58.491	53	22	0	182	234	182	234	1.78e-12	66.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056847.t1	AT1G30670	41.520	171	97	2	135	305	95	262	2.82e-29	113
MS.gene056847.t1	AT2G34820	42.254	213	115	4	97	305	81	289	2.29e-28	111
MS.gene056847.t1	AT3G21330	60.377	53	21	0	182	234	280	332	2.02e-13	70.5
MS.gene056847.t1	AT5G01305	50.000	58	29	0	182	239	48	105	1.23e-12	65.5
MS.gene056847.t1	AT4G00120	52.830	53	25	0	182	234	123	175	6.45e-12	63.9

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Find 56 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGCCAAAAGCTATGCTA+CGG	0.538423	1.1:-46149013	MS.gene056847:CDS
AAATAAAACTATTCTTGCTT+TGG	0.325467	1.1:+46147577	None:intergenic
AACGCCAAAAGCTATGCTAC+GGG	0.513263	1.1:-46149012	MS.gene056847:CDS
AAGCAAAAGGGTTAGGGTTA+GGG	0.345934	1.1:+46148918	None:intergenic
AAGGGTCTGAAACAGCTTGC+TGG	0.388228	1.1:-46147619	MS.gene056847:CDS
AAGTTGTTGGTAGTTTGAAT+AGG	0.402079	1.1:+46149304	None:intergenic
AAGTTTCTTCAAGCTCAAGT+TGG	0.567049	1.1:-46148683	MS.gene056847:CDS
AATAGTTAGAGGGAAAATGG+TGG	0.625963	1.1:+46149230	None:intergenic
AATGTGGTGACAAACTCTTT+TGG	0.390869	1.1:+46147682	None:intergenic
ACAAGTAGTTGATCTTGACA+AGG	0.633389	1.1:+46149130	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCAAATGATTACAAGCAA+GGG	+	chr1.1:46148580-46148599	None:intergenic	30.0%
AGTAGTGATTTCTAAGTTGT+TGG	+	chr1.1:46147649-46147668	None:intergenic	30.0%
ATCAAAACCTACCATTCTTA+AGG	-	chr1.1:46149299-46149318	MS.gene056847:CDS	30.0%
ATCATTATGATTGCTCAATG+TGG	+	chr1.1:46149274-46149293	None:intergenic	30.0%
TAATGCTGTTGTTGTAGATT+TGG	-	chr1.1:46148689-46148708	MS.gene056847:CDS	30.0%
TACAGTTTCTCTTGAACAAA+TGG	+	chr1.1:46149213-46149232	None:intergenic	30.0%
TACTCAAATGATTACAAGCA+AGG	+	chr1.1:46148581-46148600	None:intergenic	30.0%
TCAAAACCTACCATTCTTAA+GGG	-	chr1.1:46149300-46149319	MS.gene056847:CDS	30.0%
TCAAATAGTTAGAGGGAAAA+TGG	+	chr1.1:46147713-46147732	None:intergenic	30.0%
AAGTTTCTTCAAGCTCAAGT+TGG	-	chr1.1:46148254-46148273	MS.gene056847:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	46147585	46149374	46147585	ID=MS.gene056847
chr1.1	mRNA	46147585	46149374	46147585	ID=MS.gene056847.t1;Parent=MS.gene056847
chr1.1	exon	46148652	46149374	46148652	ID=MS.gene056847.t1.exon1;Parent=MS.gene056847.t1
chr1.1	CDS	46148652	46149374	46148652	ID=cds.MS.gene056847.t1;Parent=MS.gene056847.t1
chr1.1	exon	46147585	46147800	46147585	ID=MS.gene056847.t1.exon2;Parent=MS.gene056847.t1
chr1.1	CDS	46147585	46147800	46147585	ID=cds.MS.gene056847.t1;Parent=MS.gene056847.t1

Gene Sequence

>MS.gene056847

ATGGGAGCACTTAGCACATACTTCAACTATAATTGTTACCCTTATCAACCTATTCAAACTACCAACAACTTAGAAATCACTACTTTTCAACATCATGAACAAGAACAATTTGTGCAACAAAACCACCATTTTCCCTCTAACTATTTGAATGAAACTTCTCTTTTAGATGAAAGCTTCATTTTTCAAACTCCTTGTTTTTACTCAAATGAAACTTACCCTAACCCTTGTCAAGATCAACTACTTGTTGACTCCACTTTTTCTTCTCAAAATGATGGCTTTGTTTCAATGAATGAAATTTTCCCAAATGAGGAAGATTTCACCAATTACCTTACATGTCCAAAACGCCAAAAGCTATGCTACGGGGAGAAAAGAGAGGAACCACAACAAGAGCTTTTGAACTCAACCAATTTTTTTGTGGATGAGTTTATGACTAACCCTAACCCTAACCCTTTTGCTTCCTTTGAGGTAGAACCCTTTGCTGCTTCAAAAATTGTTGATGTTGTTCAATGTGAGAAGAAAGTTGGTGAAAGGACTATCTCGTCGCAGAGTATCGCGGCGAGAGAAAGGAGAAGGAAGATAACTGAGAAGACACAAGAGCTTGGGAAGTTGGTTCCTGGTGGACCAAAGATGAACACAGCAGAGATGCTTAATGCTGCTGCTAATTATGTCAAGTTTCTTCAAGCTCAAGTTGGAATGCTTCAACTTATGGAAACATTTAACAAGGTAACTGTTTTTGTTTTTATTGTTGCAATGTTCTTATATTATTAGAGCTCATAATTGAAGTTAATTACTTGTTTTCACTTGAGTTTCAATTTAAACTGTTTTGTGTACTTAGGTCTTATTCAATACTCCAAGTGAATTTGATGTTTTTCTTTTGAATGATGTTTTTTGCTTTTTATATGTTATGCTATGCTACTTGATTTAGTTTTTGAGTTTATAATAATACCTAGAGTTTGTTTGTTCATTGCATATTTGGTTACTTTGTGTGAAGACCCTTGCTTGTAATCATTTGAGTACATGTCGTTTGATAAAAAAATATAGAGGATATGCCATTTATTGTAGTGTTATTATGTCATGTATTGTTGTAAATATCTAAATGGATATTAATGCTGTTGTTGTAGATTTGGTTAGTTTTATCATTTATGTATGTAATCCATGACTACCTATTGTCTCAATTTTGTCTTTTTTATATTGATTTTTTTCATACCAACTTTTACTCCATGAAAATATTTAGGAATTGTTATAAAAATTGACGGATGGAATTGTTAGGTTTAAGAAAATGTTGGTTACAATGTATGAGAGAGAGAGAGAGAGACAGAGAGAGAGAGAGAGTTAAGGAAACGGTTAATAAGAAGAGGAAATGGTCAATAAACGAGAGAGTATTTAGGAACAGCAAGTGTATATCAAATGAGCTGGCTATGTAACTGTTTAACAAGGAATTTTGCAATATTTTTTTAAATTTAAATATTCACTATTTAGTGCAATATTTTTGTAAAAGCTAAATTTCTAGTGTAACTTATATAATAAAAAGAATTCACGTATATATTTTAGATTTTGCTAATTGATTATTTCAGGAAGAAAAGGAACCTCCTCCAAGTGAAGATCTACACAAACTTCTTCTTTCTCCATTTGTTCAAGAGAAACTGTATTCAGAAGAAAAGTGCTTTGTTCCAAAAGAGTTTGTCACCACATTGAGCAATCATAATGATGTTCAATCAAAACCTACCATTCTTAAGGGTCTGAAACAGCTTGCTGGGACTGAGATTGAGAAGAAACCAAAGCAAGAATAG

Protein sequence

>MS.gene056847.t1

MGALSTYFNYNCYPYQPIQTTNNLEITTFQHHEQEQFVQQNHHFPSNYLNETSLLDESFIFQTPCFYSNETYPNPCQDQLLVDSTFSSQNDGFVSMNEIFPNEEDFTNYLTCPKRQKLCYGEKREEPQQELLNSTNFFVDEFMTNPNPNPFASFEVEPFAASKIVDVVQCEKKVGERTISSQSIAARERRRKITEKTQELGKLVPGGPKMNTAEMLNAAANYVKFLQAQVGMLQLMETFNKEEKEPPPSEDLHKLLLSPFVQEKLYSEEKCFVPKEFVTTLSNHNDVQSKPTILKGLKQLAGTEIEKKPKQE