AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057149

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057149.t1 MTR_4g104020 99.034 414 4 0 290 703 108 521 0.0 853
MS.gene057149.t1 MTR_4g104020 87.402 127 15 1 1 127 1 126 4.07e-68 233
MS.gene057149.t1 MTR_8g442410 46.214 383 195 5 325 702 149 525 1.74e-101 321
MS.gene057149.t1 MTR_7g027190 41.687 403 205 8 315 703 288 674 1.48e-91 299
MS.gene057149.t1 MTR_3g065980 34.085 399 218 11 325 702 166 540 5.27e-64 222
MS.gene057149.t1 MTR_2g082090 32.323 396 244 6 317 701 196 578 6.52e-61 214
MS.gene057149.t1 MTR_3g089055 32.723 382 235 5 330 702 197 565 8.27e-60 211
MS.gene057149.t1 MTR_3g089055 32.723 382 235 5 330 702 202 570 9.50e-60 211
MS.gene057149.t1 MTR_1g029420 32.461 382 236 5 330 702 224 592 1.83e-59 211
MS.gene057149.t1 MTR_5g097480 32.292 384 238 6 328 702 174 544 6.04e-58 206
MS.gene057149.t1 MTR_5g094450 30.928 388 242 7 328 702 158 532 8.61e-56 199
MS.gene057149.t1 MTR_4g064200 32.642 386 231 9 328 701 281 649 5.88e-55 199
MS.gene057149.t1 MTR_3g056110 30.208 384 246 5 328 702 172 542 1.82e-54 196
MS.gene057149.t1 MTR_4g102790 34.109 387 234 12 325 701 286 661 2.14e-54 198
MS.gene057149.t1 MTR_4g064160 29.268 410 265 7 302 701 289 683 6.64e-54 197
MS.gene057149.t1 MTR_4g064120 29.563 389 243 8 328 700 251 624 1.04e-50 187
MS.gene057149.t1 MTR_2g097467 29.897 388 244 7 325 701 283 653 1.22e-50 187
MS.gene057149.t1 MTR_4g064150 29.397 398 250 8 320 701 350 732 3.18e-50 187
MS.gene057149.t1 MTR_4g133660 30.155 388 248 6 326 703 170 544 1.30e-49 182
MS.gene057149.t1 MTR_2g097410 30.198 404 245 10 316 701 356 740 1.82e-49 185
MS.gene057149.t1 MTR_4g064180 29.049 389 245 8 328 700 251 624 1.89e-49 184
MS.gene057149.t1 MTR_2g026250 31.458 391 239 9 326 703 223 597 5.44e-49 182
MS.gene057149.t1 MTR_7g062120 29.870 385 247 6 328 702 369 740 6.81e-49 184
MS.gene057149.t1 MTR_7g074650 31.622 370 232 8 335 702 442 792 2.10e-48 183
MS.gene057149.t1 MTR_4g076140 28.345 441 240 10 325 702 42 469 2.28e-48 177
MS.gene057149.t1 MTR_2g097473 28.974 390 249 7 323 701 281 653 9.30e-47 176
MS.gene057149.t1 MTR_2g097463 29.091 385 245 7 328 701 287 654 1.03e-46 176
MS.gene057149.t1 MTR_2g097390 28.272 382 253 8 328 701 272 640 1.76e-45 172
MS.gene057149.t1 MTR_2g097350 26.761 426 279 10 284 701 239 639 9.28e-45 170
MS.gene057149.t1 MTR_2g097310 28.982 383 249 10 328 701 269 637 1.15e-44 170
MS.gene057149.t1 MTR_1g096030 30.170 411 235 15 323 703 72 460 2.82e-44 166
MS.gene057149.t1 MTR_5g009080 28.182 440 245 13 325 703 50 479 3.49e-44 166
MS.gene057149.t1 MTR_4g076020 32.086 374 216 11 335 702 88 429 3.13e-43 162
MS.gene057149.t1 MTR_1g069725 29.302 430 261 13 294 703 58 464 4.32e-43 162
MS.gene057149.t1 MTR_3g072710 30.732 410 251 11 315 703 104 501 1.09e-42 162
MS.gene057149.t1 MTR_2g034250 29.383 405 264 9 309 702 192 585 3.66e-42 162
MS.gene057149.t1 MTR_4g077760 27.532 385 250 7 328 702 190 555 1.08e-41 160
MS.gene057149.t1 MTR_2g034260 28.501 407 264 12 309 702 192 584 2.03e-40 157
MS.gene057149.t1 MTR_3g022830 26.241 423 248 6 332 702 26 436 9.24e-40 152
MS.gene057149.t1 MTR_5g058860 29.157 415 263 10 308 703 96 498 9.31e-40 154
MS.gene057149.t1 MTR_1g086970 30.179 391 246 13 326 702 101 478 2.14e-39 152
MS.gene057149.t1 MTR_2g097380 26.005 373 228 8 336 701 229 560 1.52e-38 151
MS.gene057149.t1 MTR_2g034280 27.901 405 267 12 309 702 185 575 4.49e-38 150
MS.gene057149.t1 MTR_4g097080 26.154 390 253 10 330 702 132 503 5.70e-35 139
MS.gene057149.t1 MTR_7g109580 26.500 400 272 11 309 702 171 554 5.72e-35 140
MS.gene057149.t1 MTR_7g057230 30.592 304 199 6 406 701 357 656 2.31e-33 136
MS.gene057149.t1 MTR_5g015490 25.575 391 258 10 331 702 114 490 2.80e-32 131
MS.gene057149.t1 MTR_2g089100 25.829 422 260 11 314 701 40 442 1.76e-30 125
MS.gene057149.t1 MTR_6g047750 30.102 392 232 15 330 702 294 662 8.95e-30 125
MS.gene057149.t1 MTR_5g015950 25.891 421 254 13 330 701 34 445 4.40e-26 112
MS.gene057149.t1 MTR_3g053270 25.891 421 254 13 330 701 34 445 4.40e-26 112
MS.gene057149.t1 MTR_4g095500 26.047 430 240 14 330 701 58 467 1.99e-25 110
MS.gene057149.t1 MTR_2g099110 24.824 427 267 14 303 702 52 451 1.99e-25 110
MS.gene057149.t1 MTR_0092s0100 25.773 388 249 12 328 703 369 729 3.63e-21 99.0
MS.gene057149.t1 MTR_0092s0100 25.773 388 249 12 328 703 372 732 3.98e-21 99.0
MS.gene057149.t1 MTR_8g093070 27.366 391 236 16 328 701 136 495 4.73e-21 97.8
MS.gene057149.t1 MTR_4g026485 24.937 397 260 12 318 703 256 625 1.43e-20 96.7
MS.gene057149.t1 MTR_8g020840 24.146 410 255 13 324 702 164 548 4.98e-19 91.7
MS.gene057149.t1 MTR_3g022580 34.375 128 79 2 580 702 56 183 1.56e-17 81.6
MS.gene057149.t1 MTR_3g022005 34.653 101 66 0 602 702 25 125 1.37e-15 74.3
MS.gene057149.t1 MTR_8g077940 24.752 303 200 10 413 703 256 542 9.67e-15 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057149.t1 AT1G66350 36.554 383 215 10 325 703 149 507 1.37e-71 242
MS.gene057149.t1 AT3G03450 35.294 391 218 12 325 702 177 545 2.80e-67 231
MS.gene057149.t1 AT1G14920 36.132 393 215 11 321 702 162 529 3.81e-67 230
MS.gene057149.t1 AT5G17490 35.990 389 212 13 325 702 154 516 1.02e-66 229
MS.gene057149.t1 AT2G01570 34.351 393 222 11 321 702 214 581 5.06e-64 223
MS.gene057149.t1 AT5G48150 33.245 379 234 5 332 702 123 490 8.38e-62 215
MS.gene057149.t1 AT5G48150 33.245 379 234 5 332 702 123 490 8.38e-62 215
MS.gene057149.t1 AT5G48150 33.245 379 234 5 332 702 123 490 8.38e-62 215
MS.gene057149.t1 AT5G48150 33.245 379 234 5 332 702 123 490 8.38e-62 215
MS.gene057149.t1 AT1G21450 32.637 383 235 6 330 702 224 593 1.52e-58 208
MS.gene057149.t1 AT2G04890 31.733 375 239 5 331 702 53 413 2.02e-57 201
MS.gene057149.t1 AT2G29065 31.877 389 234 9 331 701 252 627 1.68e-54 198
MS.gene057149.t1 AT1G50600 32.031 384 239 6 328 702 156 526 6.45e-54 194
MS.gene057149.t1 AT1G50420 31.066 441 231 10 325 703 51 480 1.22e-53 192
MS.gene057149.t1 AT1G50600 32.031 384 239 6 328 702 231 601 1.27e-53 195
MS.gene057149.t1 AT1G50600 32.031 384 239 6 328 702 227 597 1.34e-53 195
MS.gene057149.t1 AT1G07520 31.620 389 234 10 331 701 154 528 2.11e-52 190
MS.gene057149.t1 AT1G07520 31.620 389 234 10 331 701 154 528 2.11e-52 190
MS.gene057149.t1 AT3G54220 34.054 370 224 9 335 701 297 649 3.87e-52 192
MS.gene057149.t1 AT3G46600 30.729 384 238 8 331 702 4 371 1.02e-51 184
MS.gene057149.t1 AT5G41920 32.564 390 241 10 317 703 31 401 1.87e-51 184
MS.gene057149.t1 AT1G07520 31.620 389 234 10 331 701 318 692 3.28e-51 190
MS.gene057149.t1 AT1G07520 31.620 389 234 10 331 701 319 693 3.55e-51 189
MS.gene057149.t1 AT5G59450 30.670 388 243 8 328 702 224 598 7.20e-50 184
MS.gene057149.t1 AT3G46600 30.729 384 238 8 331 702 180 547 8.66e-50 183
MS.gene057149.t1 AT3G46600 30.729 384 238 8 331 702 212 579 1.24e-49 183
MS.gene057149.t1 AT4G17230 31.606 386 242 6 326 702 153 525 6.15e-49 180
MS.gene057149.t1 AT4G17230 31.606 386 242 6 326 702 153 525 6.15e-49 180
MS.gene057149.t1 AT1G07530 28.385 384 253 5 328 701 393 764 3.00e-48 182
MS.gene057149.t1 AT2G37650 29.457 387 244 8 328 702 332 701 4.40e-47 178
MS.gene057149.t1 AT2G37650 29.457 387 244 8 328 702 344 713 5.34e-47 178
MS.gene057149.t1 AT2G29060 29.208 404 252 10 315 701 268 654 1.82e-46 176
MS.gene057149.t1 AT2G29060 29.208 404 252 10 315 701 305 691 3.42e-46 175
MS.gene057149.t1 AT1G55580 34.680 297 176 6 420 702 153 445 6.70e-44 164
MS.gene057149.t1 AT5G66770 28.052 385 250 7 328 702 217 584 4.69e-43 164
MS.gene057149.t1 AT1G63100 29.592 392 246 8 328 701 249 628 1.01e-42 164
MS.gene057149.t1 AT1G63100 29.592 392 246 8 328 701 274 653 1.39e-42 164
MS.gene057149.t1 AT3G50650 29.132 357 217 7 362 702 206 542 1.06e-39 154
MS.gene057149.t1 AT5G52510 29.808 312 191 8 414 702 334 640 5.66e-34 138
MS.gene057149.t1 AT4G08250 28.244 393 249 12 325 701 101 476 3.61e-33 134
MS.gene057149.t1 AT4G37650 25.622 402 251 13 331 702 146 529 3.46e-31 129
MS.gene057149.t1 AT3G49950 26.290 407 253 10 322 701 21 407 5.10e-28 117
MS.gene057149.t1 AT2G45160 26.111 360 216 14 363 703 312 640 2.95e-23 105
MS.gene057149.t1 AT3G60630 27.476 313 188 13 412 703 329 623 2.76e-22 102
MS.gene057149.t1 AT4G00150 24.860 358 223 12 370 703 220 555 2.03e-20 96.3
MS.gene057149.t1 AT4G36710 25.666 413 256 17 308 702 106 485 6.21e-18 87.8
MS.gene057149.t1 AT3G13840 25.061 411 252 16 315 702 133 510 3.57e-13 73.2

Find 132 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTGCGATTTGCGATTC+TGG 0.147690 8.1:-20487039 MS.gene057149:CDS
GTTCGTTCTGCTTGCGATTC+TGG 0.160101 8.1:-20487015 MS.gene057149:CDS
CTCAATTGTTGAGAAGAATT+TGG 0.186388 8.1:-20485500 MS.gene057149:CDS
AATTGTCCACATATTCAATT+TGG 0.204871 8.1:-20485778 MS.gene057149:CDS
CAACCGTAACTATCCCTATT+TGG 0.207024 8.1:-20487526 MS.gene057149:CDS
CTGAAATTCGCAAACCAAAT+AGG 0.213265 8.1:+20487512 None:intergenic
CTCAAAGGCTTCCAATATTA+TGG 0.226205 8.1:+20485738 None:intergenic
AATCAATTACTTCTCTTCTT+TGG 0.243141 8.1:-20487088 MS.gene057149:CDS
TGAAAATCCATCATCATTAA+TGG 0.252428 8.1:-20487678 MS.gene057149:CDS
TGTGGCTAATTCCATAATAT+TGG 0.269201 8.1:-20485749 MS.gene057149:CDS
GAGAAAGGGTGTTTGGTTCT+TGG 0.272544 8.1:-20484989 MS.gene057149:CDS
CATGATCAGAAAATGGATTA+AGG 0.277342 8.1:+20487588 None:intergenic
AAATCAAATGCTTTGGTGTT+TGG 0.284256 8.1:-20485970 MS.gene057149:CDS
GCTTGAACAACCATCTTAAT+AGG 0.288790 8.1:+20485064 None:intergenic
ATTTCTTTCTCAGCGTTAAA+AGG 0.289911 8.1:+20487309 None:intergenic
ATCTCAAATCTCATATTAAC+AGG 0.292368 8.1:-20487187 MS.gene057149:CDS
TCAGTTTCATGATCAGAAAA+TGG 0.293107 8.1:+20487581 None:intergenic
TTCTGCTATATTTGACTCAT+TGG 0.296218 8.1:-20485254 MS.gene057149:CDS
TATTGAAGAAACTATTACTA+TGG 0.299092 8.1:+20487486 None:intergenic
GTTATGGCTGAGCAAGATTC+TGG 0.301485 8.1:-20485328 MS.gene057149:CDS
TTTCTTCATGTGGTTGATCT+TGG 0.314063 8.1:-20485706 MS.gene057149:CDS
TTCATCTACCTCACATTTGA+AGG 0.343224 8.1:+20487697 None:intergenic
CAGCAAGAAACTGCAACAAT+AGG 0.367426 8.1:+20484953 None:intergenic
CTATTGTTGCAGTTTCTTGC+TGG 0.367470 8.1:-20484952 MS.gene057149:CDS
CATAATATTGGAAGCCTTTG+AGG 0.384786 8.1:-20485737 MS.gene057149:CDS
TGAAATTCGCAAACCAAATA+GGG 0.387079 8.1:+20487513 None:intergenic
ATCGATCCAGCCACCGTGTT+CGG 0.388370 8.1:-20485624 MS.gene057149:CDS
GGATGCTGCATCAGAAGAAA+TGG 0.398391 8.1:-20485827 MS.gene057149:CDS
CCAGCAGAGTTATTACCAAT+TGG 0.412468 8.1:+20485880 None:intergenic
AAAATCAATGATGAACATAA+TGG 0.412525 8.1:-20485848 MS.gene057149:CDS
CGAGCTTAAATCAAATGCTT+TGG 0.422403 8.1:-20485977 MS.gene057149:CDS
GAAAATCCATCATCATTAAT+GGG 0.422874 8.1:-20487677 MS.gene057149:CDS
GTTGATCTTGGCATGAGCCT+TGG 0.423272 8.1:-20485694 MS.gene057149:CDS
TCAGCCATAACCAACACTTT+TGG 0.423891 8.1:+20485340 None:intergenic
CGCCCGCGAAACAGAAGAAC+AGG 0.424849 8.1:-20487225 MS.gene057149:CDS
TTTCTTTCTCAGCGTTAAAA+GGG 0.425888 8.1:+20487310 None:intergenic
AGGAGGAGAATGAGTAGGGC+TGG 0.435845 8.1:-20485100 MS.gene057149:CDS
TTCGCCAAAAGTGTTGGTTA+TGG 0.438665 8.1:-20485344 MS.gene057149:CDS
AAACGCCGTTACGGCCGTTA+CGG 0.439610 8.1:-20487277 MS.gene057149:CDS
TCTGCTTGCGTCTCCTAGGC+AGG 0.440116 8.1:-20486883 MS.gene057149:intron
ATGAGTAGGGCTGGATTTCA+AGG 0.440915 8.1:-20485091 MS.gene057149:CDS
CAATTACTTCTCTTCTTTGG+TGG 0.444688 8.1:-20487085 MS.gene057149:CDS
AAGCTGCCGAACACGGTGGC+TGG 0.447890 8.1:+20485618 None:intergenic
AAGGGTGTTTGGTTCTTGGA+TGG 0.458218 8.1:-20484985 MS.gene057149:CDS
TTTGGAGAATTTGAAACCTA+AGG 0.459147 8.1:-20485482 MS.gene057149:CDS
TCATGCCTCTCCATCCTCAA+TGG 0.459152 8.1:+20485139 None:intergenic
TAAGAACCCATTAATGATGA+TGG 0.459556 8.1:+20487671 None:intergenic
CACTTCTGCTTGCGTCTCCT+AGG 0.463495 8.1:-20486887 MS.gene057149:CDS
TCAAGGTTCACCTATTAAGA+TGG 0.464234 8.1:-20485074 MS.gene057149:CDS
CTAACTCTGATTCAACCAAT+TGG 0.465189 8.1:-20485895 MS.gene057149:CDS
CTCCTGTTCTTCTGTTTCGC+GGG 0.469158 8.1:+20487223 None:intergenic
TGTGGAAGAGAAAGGGTGTT+TGG 0.469216 8.1:-20484996 MS.gene057149:CDS
GCCACCGTGTTCGGCAGCTT+AGG 0.471055 8.1:-20485615 MS.gene057149:CDS
AACAGAAGAACAGGAGAAGA+AGG 0.471853 8.1:-20487216 MS.gene057149:CDS
CTTCTGTTTCGCGGGCGTAA+CGG 0.472327 8.1:+20487231 None:intergenic
AATATCTTTGCTTATAAGCA+AGG 0.483710 8.1:+20486169 None:intergenic
GCCCTCTTAGTGTCATACTT+TGG 0.484118 8.1:+20485220 None:intergenic
AAATGTCCAAATTGAATATG+TGG 0.495894 8.1:+20485772 None:intergenic
TGCCAAAGTATGACACTAAG+AGG 0.499635 8.1:-20485222 MS.gene057149:CDS
TATACTGTTGTGGAAGAGAA+AGG 0.500286 8.1:-20485004 MS.gene057149:CDS
AGAGTTCTTCACCAATGACT+TGG 0.501290 8.1:+20485560 None:intergenic
TCGCGACAAATCACACGCTT+CGG 0.504012 8.1:-20486010 MS.gene057149:CDS
TCTCATATTAACAGGGGAAG+AGG 0.506339 8.1:-20487179 MS.gene057149:CDS
ATAATATTGGAAGCCTTTGA+GGG 0.506937 8.1:-20485736 MS.gene057149:CDS
CCAATTGGTAATAACTCTGC+TGG 0.507639 8.1:-20485880 MS.gene057149:CDS
AAGAACATTGTGAGCTGTGA+AGG 0.508905 8.1:-20485163 MS.gene057149:CDS
TCTCCTGTTCTTCTGTTTCG+CGG 0.518417 8.1:+20487222 None:intergenic
AAGAACAGGAGAAGAAGGAC+AGG 0.518537 8.1:-20487211 MS.gene057149:CDS
CAATTTCTTGTGATCCCTAG+TGG 0.519092 8.1:+20487556 None:intergenic
ATTGCTAGAATCCAAGTCAT+TGG 0.519171 8.1:-20485571 MS.gene057149:CDS
GTCGTAACGGCGTTCCGTAA+CGG 0.519248 8.1:+20487263 None:intergenic
TGATCTCTGAAATGAAGCCT+TGG 0.520426 8.1:+20487428 None:intergenic
AGAAAGTTGATCAGTGGAGG+AGG 0.527630 8.1:-20485117 MS.gene057149:CDS
CAAAGCATTTGATTTAAGCT+CGG 0.529084 8.1:+20485978 None:intergenic
ACGGCATTTCGCGGTCGTAA+CGG 0.537205 8.1:+20487250 None:intergenic
TTTGAATTAGTCCTCGCCAT+TGG 0.537234 8.1:+20485656 None:intergenic
GCGGGCGTAACGGCATTTCG+CGG 0.539435 8.1:+20487241 None:intergenic
CACATAAGAACTTATAAGCA+AGG 0.539564 8.1:-20487407 MS.gene057149:CDS
ATACTGTTGTGGAAGAGAAA+GGG 0.539638 8.1:-20485003 MS.gene057149:CDS
AAAGATTTGAGATGCCTGGC+TGG 0.543114 8.1:+20487140 None:intergenic
AAGAAAACTCTCACCCTCAA+AGG 0.545146 8.1:+20485723 None:intergenic
AATGGAAGAAGCATTCAAGT+TGG 0.548963 8.1:-20485809 MS.gene057149:CDS
TTACAAATCGGTATCGGCAG+TGG 0.550910 8.1:-20487349 MS.gene057149:CDS
ATGATTCCATAAACCTTCCA+AGG 0.552621 8.1:+20485287 None:intergenic
AGTGGAGGAGGAGAATGAGT+AGG 0.556868 8.1:-20485105 MS.gene057149:CDS
CATAAAAGTGAATGAAAATG+AGG 0.557353 8.1:-20485458 MS.gene057149:CDS
CACTTATCACAAACCTGCCT+AGG 0.559799 8.1:+20486870 None:intergenic
CAGAATCGCAAATCGCAAGC+AGG 0.561964 8.1:+20487040 None:intergenic
GTGAAGAATAATGTTTGTGA+TGG 0.562851 8.1:-20485028 MS.gene057149:CDS
GTGGAGGAGGAGAATGAGTA+GGG 0.563946 8.1:-20485104 MS.gene057149:CDS
GGCATGAGAAAGTTGATCAG+TGG 0.565335 8.1:-20485123 MS.gene057149:CDS
AATTGCGAAGCTGAAGAAGA+TGG 0.566408 8.1:-20486096 MS.gene057149:CDS
CGGTCTTTCGCCAAAAGTGT+TGG 0.568749 8.1:-20485350 MS.gene057149:CDS
TGCTAGTACCTTCAAATGTG+AGG 0.569925 8.1:-20487705 MS.gene057149:CDS
GCGTTCCGTAACGGCCGTAA+CGG 0.570906 8.1:+20487272 None:intergenic
GTCTTCCACATGGACACCAA+TGG 0.579517 8.1:-20485672 MS.gene057149:CDS
TACAAAAGATTTGAGATGCC+TGG 0.582467 8.1:+20487136 None:intergenic
AAACCAAATAGGGATAGTTA+CGG 0.582805 8.1:+20487523 None:intergenic
CAAATCGCAAGCAGGAAGGA+AGG 0.584416 8.1:+20487048 None:intergenic
CAGCTTAGGATCACAGCGAT+TGG 0.586462 8.1:-20485601 MS.gene057149:CDS
GAAGATGGTTCATGTGCTGA+TGG 0.596375 8.1:-20486081 MS.gene057149:CDS
AAGGTACTAGCATAAAGTGC+AGG 0.596660 8.1:+20487716 None:intergenic
CGGCCGTTACAAATCGGTAT+CGG 0.601274 8.1:-20487355 MS.gene057149:CDS
ATGAGAAAGTTGATCAGTGG+AGG 0.606341 8.1:-20485120 MS.gene057149:CDS
TTCTTTCTCAGCGTTAAAAG+GGG 0.606492 8.1:+20487311 None:intergenic
TGATCCTAAGCTGCCGAACA+CGG 0.610159 8.1:+20485611 None:intergenic
ATCATGAAACTGAAACCACT+AGG 0.610327 8.1:-20487571 MS.gene057149:CDS
CTGTGAAGGACCATTGAGGA+TGG 0.613485 8.1:-20485149 MS.gene057149:CDS
GAGTCGTGGCGCTTTGAATG+CGG 0.617247 8.1:-20485389 MS.gene057149:CDS
AAGGACCATTGAGGATGGAG+AGG 0.621814 8.1:-20485144 MS.gene057149:CDS
ATCGCAAATCGCAAGCAGGA+AGG 0.626390 8.1:+20487044 None:intergenic
TCATGAAACTGAAACCACTA+GGG 0.631575 8.1:-20487570 MS.gene057149:CDS
ATGAGCCTTGGTCTTCCACA+TGG 0.634567 8.1:-20485682 MS.gene057149:CDS
AAAACAGTGCTAGTTCAACA+TGG 0.638515 8.1:-20487613 MS.gene057149:CDS
CTCAAATCTCATATTAACAG+GGG 0.639241 8.1:-20487185 MS.gene057149:CDS
GAGCAAGATTCTGGTCACAA+TGG 0.639301 8.1:-20485319 MS.gene057149:CDS
TTGTGATGGATATACTGTTG+TGG 0.639465 8.1:-20485014 MS.gene057149:CDS
TGGTTGTTCAAGCTAAGCAG+TGG 0.641582 8.1:-20485054 MS.gene057149:CDS
ACAGTGTGAATAGCATTCCA+AGG 0.646338 8.1:-20487445 MS.gene057149:CDS
GGTGTCCATGTGGAAGACCA+AGG 0.647949 8.1:+20485677 None:intergenic
CCTCGCCATTGGTGTCCATG+TGG 0.649204 8.1:+20485667 None:intergenic
GCCAAAGTATGACACTAAGA+GGG 0.650623 8.1:-20485221 MS.gene057149:CDS
TGTGTTGTTAAAGAGAGTCG+TGG 0.651261 8.1:-20485403 MS.gene057149:CDS
CTGCCGATACCGATTTGTAA+CGG 0.655656 8.1:+20487352 None:intergenic
ACGCAAGCAGAAGTGCAGAA+CGG 0.658317 8.1:+20486895 None:intergenic
TCTCAAATCTCATATTAACA+GGG 0.659644 8.1:-20487186 MS.gene057149:CDS
CCACATGGACACCAATGGCG+AGG 0.669979 8.1:-20485667 MS.gene057149:CDS
GAATGAAAATGAGGTCCTTG+TGG 0.675244 8.1:-20485449 MS.gene057149:CDS
GAAGAATGCTGTTAACCACA+AGG 0.689238 8.1:+20485434 None:intergenic
AGGACTAATTCAAAGTCTCG+CGG 0.704228 8.1:-20485647 MS.gene057149:CDS
TGAGCTGTGAAGGACCATTG+AGG 0.705754 8.1:-20485153 MS.gene057149:CDS
TCCTAAGCTGCCGAACACGG+TGG 0.761080 8.1:+20485614 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATTACTTTTTTTTTGA+AGG - chr8.1:20485960-20485979 MS.gene057149:CDS 10.0%
!!! AATTAATAATTACTTTTAGA+TGG - chr8.1:20485984-20486003 MS.gene057149:CDS 10.0%
!! CTTTAAACATGTTATAATTA+TGG - chr8.1:20486289-20486308 MS.gene057149:intron 15.0%
!!! GAAATTTTAGAGAAAAAATT+GGG - chr8.1:20486138-20486157 MS.gene057149:CDS 15.0%
!!! TGAAATTTTAGAGAAAAAAT+TGG - chr8.1:20486137-20486156 MS.gene057149:CDS 15.0%
!! AAAATCAATGATGAACATAA+TGG - chr8.1:20486817-20486836 MS.gene057149:intron 20.0%
!! TATTGAAGAAACTATTACTA+TGG + chr8.1:20485182-20485201 None:intergenic 20.0%
!!! ATAAGCAAGGTTTTAAAAAA+CGG - chr8.1:20485271-20485290 MS.gene057149:CDS 20.0%
!!! TTTTCTAAAATAGGCAAATT+TGG + chr8.1:20485036-20485055 None:intergenic 20.0%
! AAATGTCCAAATTGAATATG+TGG + chr8.1:20486896-20486915 None:intergenic 25.0%
! AATATCTTTGCTTATAAGCA+AGG + chr8.1:20486499-20486518 None:intergenic 25.0%
! AATCAATTACTTCTCTTCTT+TGG - chr8.1:20485577-20485596 MS.gene057149:CDS 25.0%
! AATTGTCCACATATTCAATT+TGG - chr8.1:20486887-20486906 MS.gene057149:CDS 25.0%
! ATCTCAAATCTCATATTAAC+AGG - chr8.1:20485478-20485497 MS.gene057149:CDS 25.0%
! CATAAAAGTGAATGAAAATG+AGG - chr8.1:20487207-20487226 MS.gene057149:CDS 25.0%
! GAAAATCCATCATCATTAAT+GGG - chr8.1:20484988-20485007 MS.gene057149:CDS 25.0%
! TCAATTTACGCGAAAAATTT+GGG - chr8.1:20487127-20487146 MS.gene057149:CDS 25.0%
! TCTCAAATCTCATATTAACA+GGG - chr8.1:20485479-20485498 MS.gene057149:CDS 25.0%
! TGAAAATCCATCATCATTAA+TGG - chr8.1:20484987-20485006 MS.gene057149:CDS 25.0%
!! CACTCTTAATTTGAGTATTT+AGG - chr8.1:20486100-20486119 MS.gene057149:CDS 25.0%
!! TATTCAATTTGGACATTTTG+TGG - chr8.1:20486898-20486917 MS.gene057149:CDS 25.0%
!! TCATCTCTTATTTAAGTTCA+AGG - chr8.1:20486063-20486082 MS.gene057149:CDS 25.0%
!! TGTAAAAAAGATTTGAGATG+TGG + chr8.1:20485555-20485574 None:intergenic 25.0%
!!! ATAAGCAAGGTTCTAAAAAA+CGG + chr8.1:20486486-20486505 None:intergenic 25.0%
!!! TAAGCAAGGTTTTAAAAAAC+GGG - chr8.1:20485272-20485291 MS.gene057149:CDS 25.0%
!!! TTTTGTTTTTTGAGCGAATA+AGG - chr8.1:20485827-20485846 MS.gene057149:CDS 25.0%
AAACCAAATAGGGATAGTTA+CGG + chr8.1:20485145-20485164 None:intergenic 30.0%
ATAAACCTTCCAAGGAAAAA+AGG + chr8.1:20487373-20487392 None:intergenic 30.0%
ATAATATTGGAAGCCTTTGA+GGG - chr8.1:20486929-20486948 MS.gene057149:CDS 30.0%
ATTTCTTTCTCAGCGTTAAA+AGG + chr8.1:20485359-20485378 None:intergenic 30.0%
CAATTTACGCGAAAAATTTG+GGG - chr8.1:20487128-20487147 MS.gene057149:CDS 30.0%
CACATAAGAACTTATAAGCA+AGG - chr8.1:20485258-20485277 MS.gene057149:CDS 30.0%
CTCAAATCTCATATTAACAG+GGG - chr8.1:20485480-20485499 MS.gene057149:CDS 30.0%
CTCAATTGTTGAGAAGAATT+TGG - chr8.1:20487165-20487184 MS.gene057149:CDS 30.0%
CTCAATTTACGCGAAAAATT+TGG - chr8.1:20487126-20487145 MS.gene057149:CDS 30.0%
GAAAAATTTGGGGATTCATT+TGG - chr8.1:20487138-20487157 MS.gene057149:CDS 30.0%
GTGAAGAATAATGTTTGTGA+TGG - chr8.1:20487637-20487656 MS.gene057149:CDS 30.0%
TAAGAACCCATTAATGATGA+TGG + chr8.1:20484997-20485016 None:intergenic 30.0%
TCAGTTTCATGATCAGAAAA+TGG + chr8.1:20485087-20485106 None:intergenic 30.0%
TGAAATTCGCAAACCAAATA+GGG + chr8.1:20485155-20485174 None:intergenic 30.0%
TGTGGCTAATTCCATAATAT+TGG - chr8.1:20486916-20486935 MS.gene057149:CDS 30.0%
TTCTGCTATATTTGACTCAT+TGG - chr8.1:20487411-20487430 MS.gene057149:CDS 30.0%
TTTCTTTCTCAGCGTTAAAA+GGG + chr8.1:20485358-20485377 None:intergenic 30.0%
! CAAAGCATTTGATTTAAGCT+CGG + chr8.1:20486690-20486709 None:intergenic 30.0%
! TTTGGAGAATTTGAAACCTA+AGG - chr8.1:20487183-20487202 MS.gene057149:CDS 30.0%
!! AAATCAAATGCTTTGGTGTT+TGG - chr8.1:20486695-20486714 MS.gene057149:intron 30.0%
!! CATGATCAGAAAATGGATTA+AGG + chr8.1:20485080-20485099 None:intergenic 30.0%
!! TCATTCACTTTTATGTCCTT+AGG + chr8.1:20487202-20487221 None:intergenic 30.0%
!!! CTAGCACTGTTTTCTAAAAT+AGG + chr8.1:20485045-20485064 None:intergenic 30.0%
!!! TTTTTTCCTTGGAAGGTTTA+TGG - chr8.1:20487372-20487391 MS.gene057149:CDS 30.0%
AAAACAGTGCTAGTTCAACA+TGG - chr8.1:20485052-20485071 MS.gene057149:CDS 35.0%
ATACTGTTGTGGAAGAGAAA+GGG - chr8.1:20487662-20487681 MS.gene057149:CDS 35.0%
ATCATGAAACTGAAACCACT+AGG - chr8.1:20485094-20485113 MS.gene057149:CDS 35.0%
ATGATTCCATAAACCTTCCA+AGG + chr8.1:20487381-20487400 None:intergenic 35.0%
ATTGCTAGAATCCAAGTCAT+TGG - chr8.1:20487094-20487113 MS.gene057149:CDS 35.0%
CAATTACTTCTCTTCTTTGG+TGG - chr8.1:20485580-20485599 MS.gene057149:CDS 35.0%
CATAATATTGGAAGCCTTTG+AGG - chr8.1:20486928-20486947 MS.gene057149:CDS 35.0%
CGAGCTTAAATCAAATGCTT+TGG - chr8.1:20486688-20486707 MS.gene057149:intron 35.0%
CTAACTCTGATTCAACCAAT+TGG - chr8.1:20486770-20486789 MS.gene057149:intron 35.0%
CTCAAAGGCTTCCAATATTA+TGG + chr8.1:20486930-20486949 None:intergenic 35.0%
CTGAAATTCGCAAACCAAAT+AGG + chr8.1:20485156-20485175 None:intergenic 35.0%
GCTTGAACAACCATCTTAAT+AGG + chr8.1:20487604-20487623 None:intergenic 35.0%
TATACTGTTGTGGAAGAGAA+AGG - chr8.1:20487661-20487680 MS.gene057149:CDS 35.0%
TCAAGGTTCACCTATTAAGA+TGG - chr8.1:20487591-20487610 MS.gene057149:CDS 35.0%
TCATGAAACTGAAACCACTA+GGG - chr8.1:20485095-20485114 MS.gene057149:CDS 35.0%
TTCATCTACCTCACATTTGA+AGG + chr8.1:20484971-20484990 None:intergenic 35.0%
TTCTTTCTCAGCGTTAAAAG+GGG + chr8.1:20485357-20485376 None:intergenic 35.0%
TTTCTTCATGTGGTTGATCT+TGG - chr8.1:20486959-20486978 MS.gene057149:CDS 35.0%
! TACAAAAGATTTGAGATGCC+TGG + chr8.1:20485532-20485551 None:intergenic 35.0%
!! AATGGAAGAAGCATTCAAGT+TGG - chr8.1:20486856-20486875 MS.gene057149:intron 35.0%
!! TTGTGATGGATATACTGTTG+TGG - chr8.1:20487651-20487670 MS.gene057149:CDS 35.0%
!!! GGAACAGTTTTATTTTGCTG+AGG - chr8.1:20487474-20487493 MS.gene057149:CDS 35.0%
!!! GTTGCTTCTTGTTTTGTTCA+AGG - chr8.1:20486734-20486753 MS.gene057149:intron 35.0%
AAAAGGGGCAAAAACGGTAA+CGG + chr8.1:20485342-20485361 None:intergenic 40.0%
AACAGAAGAACAGGAGAAGA+AGG - chr8.1:20485449-20485468 MS.gene057149:CDS 40.0%
AAGAAAACTCTCACCCTCAA+AGG + chr8.1:20486945-20486964 None:intergenic 40.0%
AAGAACATTGTGAGCTGTGA+AGG - chr8.1:20487502-20487521 MS.gene057149:CDS 40.0%
AATTGCGAAGCTGAAGAAGA+TGG - chr8.1:20486569-20486588 MS.gene057149:intron 40.0%
ACAGTGTGAATAGCATTCCA+AGG - chr8.1:20485220-20485239 MS.gene057149:CDS 40.0%
AGAGTTCTTCACCAATGACT+TGG + chr8.1:20487108-20487127 None:intergenic 40.0%
AGCGTTAAAAGGGGCAAAAA+CGG + chr8.1:20485348-20485367 None:intergenic 40.0%
AGGACTAATTCAAAGTCTCG+CGG - chr8.1:20487018-20487037 MS.gene057149:CDS 40.0%
CAAAAACGGCCGTTACAAAT+CGG - chr8.1:20485304-20485323 MS.gene057149:CDS 40.0%
CAACCGTAACTATCCCTATT+TGG - chr8.1:20485139-20485158 MS.gene057149:CDS 40.0%
CAATTTCTTGTGATCCCTAG+TGG + chr8.1:20485112-20485131 None:intergenic 40.0%
CAGCAAGAAACTGCAACAAT+AGG + chr8.1:20487715-20487734 None:intergenic 40.0%
CCAGCAGAGTTATTACCAAT+TGG + chr8.1:20486788-20486807 None:intergenic 40.0%
CTATTGTTGCAGTTTCTTGC+TGG - chr8.1:20487713-20487732 MS.gene057149:CDS 40.0%
GAAGAATGCTGTTAACCACA+AGG + chr8.1:20487234-20487253 None:intergenic 40.0%
GAATGAAAATGAGGTCCTTG+TGG - chr8.1:20487216-20487235 MS.gene057149:CDS 40.0%
GATACAAATAACGGTCTTCG+CGG + chr8.1:20486403-20486422 None:intergenic 40.0%
GCCAAAGTATGACACTAAGA+GGG - chr8.1:20487444-20487463 MS.gene057149:CDS 40.0%
TCACAGAAAAAACGCCGTTA+CGG - chr8.1:20485379-20485398 MS.gene057149:CDS 40.0%
TCTCATATTAACAGGGGAAG+AGG - chr8.1:20485486-20485505 MS.gene057149:CDS 40.0%
TGACACTAAGAGGGCAAAAA+TGG - chr8.1:20487453-20487472 MS.gene057149:CDS 40.0%
TGCCAAAGTATGACACTAAG+AGG - chr8.1:20487443-20487462 MS.gene057149:CDS 40.0%
TGCTAGTACCTTCAAATGTG+AGG - chr8.1:20484960-20484979 MS.gene057149:CDS 40.0%
TTTGAATTAGTCCTCGCCAT+TGG + chr8.1:20487012-20487031 None:intergenic 40.0%
! AAGGTACTAGCATAAAGTGC+AGG + chr8.1:20484952-20484971 None:intergenic 40.0%
! ATGAGAAAGTTGATCAGTGG+AGG - chr8.1:20487545-20487564 MS.gene057149:CDS 40.0%
! CCAATTGGTAATAACTCTGC+TGG - chr8.1:20486785-20486804 MS.gene057149:intron 40.0%
! GCGGTTTTACAAATGATCCA+CGG - chr8.1:20487295-20487314 MS.gene057149:CDS 40.0%
! TCAGCCATAACCAACACTTT+TGG + chr8.1:20487328-20487347 None:intergenic 40.0%
! TCTTATCTTTTGTACCAGCC+AGG - chr8.1:20485511-20485530 MS.gene057149:CDS 40.0%
! TGTGTTGTTAAAGAGAGTCG+TGG - chr8.1:20487262-20487281 MS.gene057149:CDS 40.0%
!! AAGGTTCTAAAAAACGGTCG+CGG + chr8.1:20486480-20486499 None:intergenic 40.0%
!! TGATCTCTGAAATGAAGCCT+TGG + chr8.1:20485240-20485259 None:intergenic 40.0%
!! TTCGCCAAAAGTGTTGGTTA+TGG - chr8.1:20487321-20487340 MS.gene057149:CDS 40.0%
!!! ATGGACCTTTTTTCCTTGGA+AGG - chr8.1:20487365-20487384 MS.gene057149:CDS 40.0%
!!! ATTTGTAACGGCCGTTTTTG+CGG + chr8.1:20485304-20485323 None:intergenic 40.0%
!!! CACAATGGACCTTTTTTCCT+TGG - chr8.1:20487361-20487380 MS.gene057149:CDS 40.0%
AAGAACAGGAGAAGAAGGAC+AGG - chr8.1:20485454-20485473 MS.gene057149:CDS 45.0%
ATGAGTAGGGCTGGATTTCA+AGG - chr8.1:20487574-20487593 MS.gene057149:CDS 45.0%
CACTTATCACAAACCTGCCT+AGG + chr8.1:20485798-20485817 None:intergenic 45.0%
CGTTAAGGTTGTCGCGATTT+CGG + chr8.1:20486442-20486461 None:intergenic 45.0%
GAAGATGGTTCATGTGCTGA+TGG - chr8.1:20486584-20486603 MS.gene057149:intron 45.0%
GAGCAAGATTCTGGTCACAA+TGG - chr8.1:20487346-20487365 MS.gene057149:CDS 45.0%
GCCCTCTTAGTGTCATACTT+TGG + chr8.1:20487448-20487467 None:intergenic 45.0%
GGACGCTGCGATACAAATAA+CGG + chr8.1:20486412-20486431 None:intergenic 45.0%
GGATGCTGCATCAGAAGAAA+TGG - chr8.1:20486838-20486857 MS.gene057149:intron 45.0%
GTTATGGCTGAGCAAGATTC+TGG - chr8.1:20487337-20487356 MS.gene057149:CDS 45.0%
TCTCCTGTTCTTCTGTTTCG+CGG + chr8.1:20485446-20485465 None:intergenic 45.0%
TGGTTGTTCAAGCTAAGCAG+TGG - chr8.1:20487611-20487630 MS.gene057149:CDS 45.0%
! AAAGATTTGAGATGCCTGGC+TGG + chr8.1:20485528-20485547 None:intergenic 45.0%
! AGAAAGTTGATCAGTGGAGG+AGG - chr8.1:20487548-20487567 MS.gene057149:CDS 45.0%
! GGCATGAGAAAGTTGATCAG+TGG - chr8.1:20487542-20487561 MS.gene057149:CDS 45.0%
! GGGTGAGAGTTTTCTTCATG+TGG - chr8.1:20486949-20486968 MS.gene057149:CDS 45.0%
!! AAGGGTGTTTGGTTCTTGGA+TGG - chr8.1:20487680-20487699 MS.gene057149:CDS 45.0%
!! CTGCCGATACCGATTTGTAA+CGG + chr8.1:20485316-20485335 None:intergenic 45.0%
!! GAGAAAGGGTGTTTGGTTCT+TGG - chr8.1:20487676-20487695 MS.gene057149:CDS 45.0%
!! TGTGGAAGAGAAAGGGTGTT+TGG - chr8.1:20487669-20487688 MS.gene057149:CDS 45.0%
!! TTACAAATCGGTATCGGCAG+TGG - chr8.1:20485316-20485335 MS.gene057149:CDS 45.0%
AAGGACCATTGAGGATGGAG+AGG - chr8.1:20487521-20487540 MS.gene057149:CDS 50.0%
ACGGGACGTTACCGCAAAAA+CGG - chr8.1:20485290-20485309 MS.gene057149:CDS 50.0%
AGTGGAGGAGGAGAATGAGT+AGG - chr8.1:20487560-20487579 MS.gene057149:CDS 50.0%
ATCGCAAATCGCAAGCAGGA+AGG + chr8.1:20485624-20485643 None:intergenic 50.0%
CAAATCGCAAGCAGGAAGGA+AGG + chr8.1:20485620-20485639 None:intergenic 50.0%
CAGAATCGCAAATCGCAAGC+AGG + chr8.1:20485628-20485647 None:intergenic 50.0%
CAGCTTAGGATCACAGCGAT+TGG - chr8.1:20487064-20487083 MS.gene057149:CDS 50.0%
CGGCCGTTACAAATCGGTAT+CGG - chr8.1:20485310-20485329 MS.gene057149:CDS 50.0%
CTCCTGTTCTTCTGTTTCGC+GGG + chr8.1:20485445-20485464 None:intergenic 50.0%
CTGCTTGCGATTTGCGATTC+TGG - chr8.1:20485626-20485645 MS.gene057149:CDS 50.0%
CTGTGAAGGACCATTGAGGA+TGG - chr8.1:20487516-20487535 MS.gene057149:CDS 50.0%
GTCTTCCACATGGACACCAA+TGG - chr8.1:20486993-20487012 MS.gene057149:CDS 50.0%
GTGGAGGAGGAGAATGAGTA+GGG - chr8.1:20487561-20487580 MS.gene057149:CDS 50.0%
GTTCGTTCTGCTTGCGATTC+TGG - chr8.1:20485650-20485669 MS.gene057149:CDS 50.0%
GTTGATCTTGGCATGAGCCT+TGG - chr8.1:20486971-20486990 MS.gene057149:CDS 50.0%
TCATGCCTCTCCATCCTCAA+TGG + chr8.1:20487529-20487548 None:intergenic 50.0%
TCGCGACAAATCACACGCTT+CGG - chr8.1:20486655-20486674 MS.gene057149:intron 50.0%
TGAGCTGTGAAGGACCATTG+AGG - chr8.1:20487512-20487531 MS.gene057149:CDS 50.0%
TGATCCTAAGCTGCCGAACA+CGG + chr8.1:20487057-20487076 None:intergenic 50.0%
TGTCGCGATTTCGGCTGTAA+CGG + chr8.1:20486433-20486452 None:intergenic 50.0%
! ACACTTTTGGCGAAAGACCG+TGG + chr8.1:20487315-20487334 None:intergenic 50.0%
! ACGCAAGCAGAAGTGCAGAA+CGG + chr8.1:20485739-20485758 None:intergenic 50.0%
! ATGAGCCTTGGTCTTCCACA+TGG - chr8.1:20486983-20487002 MS.gene057149:CDS 50.0%
! CGGTCTTTCGCCAAAAGTGT+TGG - chr8.1:20487315-20487334 MS.gene057149:CDS 50.0%
AAACGCCGTTACGGCCGTTA+CGG - chr8.1:20485388-20485407 MS.gene057149:CDS 55.0%
ACGGCATTTCGCGGTCGTAA+CGG + chr8.1:20485418-20485437 None:intergenic 55.0%
AGGAGGAGAATGAGTAGGGC+TGG - chr8.1:20487565-20487584 MS.gene057149:CDS 55.0%
ATCGATCCAGCCACCGTGTT+CGG - chr8.1:20487041-20487060 MS.gene057149:CDS 55.0%
CACTTCTGCTTGCGTCTCCT+AGG - chr8.1:20485778-20485797 MS.gene057149:CDS 55.0%
CTTCTGTTTCGCGGGCGTAA+CGG + chr8.1:20485437-20485456 None:intergenic 55.0%
GGTGTCCATGTGGAAGACCA+AGG + chr8.1:20486991-20487010 None:intergenic 55.0%
GTCGTAACGGCGTTCCGTAA+CGG + chr8.1:20485405-20485424 None:intergenic 55.0%
! GAGTCGTGGCGCTTTGAATG+CGG - chr8.1:20487276-20487295 MS.gene057149:CDS 55.0%
CCACATGGACACCAATGGCG+AGG - chr8.1:20486998-20487017 MS.gene057149:CDS 60.0%
CGCCCGCGAAACAGAAGAAC+AGG - chr8.1:20485440-20485459 MS.gene057149:CDS 60.0%
GCGTTCCGTAACGGCCGTAA+CGG + chr8.1:20485396-20485415 None:intergenic 60.0%
TCCTAAGCTGCCGAACACGG+TGG + chr8.1:20487054-20487073 None:intergenic 60.0%
TCTGCTTGCGTCTCCTAGGC+AGG - chr8.1:20485782-20485801 MS.gene057149:CDS 60.0%
! CCTCGCCATTGGTGTCCATG+TGG + chr8.1:20487001-20487020 None:intergenic 60.0%
AAGCTGCCGAACACGGTGGC+TGG + chr8.1:20487050-20487069 None:intergenic 65.0%
GCCACCGTGTTCGGCAGCTT+AGG - chr8.1:20487050-20487069 MS.gene057149:CDS 65.0%
GCGGGCGTAACGGCATTTCG+CGG + chr8.1:20485427-20485446 None:intergenic 65.0%
TCGCGATCGCGGTCGCGTTA+AGG + chr8.1:20486457-20486476 None:intergenic 65.0%
AACGGTCGCGGTCGCGATCG+CGG + chr8.1:20486468-20486487 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 20484943 20487744 20484943 ID=MS.gene057149
chr8.1 mRNA 20484943 20487744 20484943 ID=MS.gene057149.t1;Parent=MS.gene057149
chr8.1 exon 20486884 20487744 20486884 ID=MS.gene057149.t1.exon1;Parent=MS.gene057149.t1
chr8.1 CDS 20486884 20487744 20486884 ID=cds.MS.gene057149.t1;Parent=MS.gene057149.t1
chr8.1 exon 20484943 20486193 20484943 ID=MS.gene057149.t1.exon2;Parent=MS.gene057149.t1
chr8.1 CDS 20484943 20486193 20484943 ID=cds.MS.gene057149.t1;Parent=MS.gene057149.t1
Gene Sequence

>MS.gene057149

ATGTCACCTGCACTTTATGCTAGTACCTTCAAATGTGAGGTAGATGAAAATCCATCATCATTAATGGGTTCTTACTATGCTTCTTTATATCCAAATTTGCCTATTTTAGAAAACAGTGCTAGTTCAACATGGATCCTTAATCCATTTTCTGATCATGAAACTGAAACCACTAGGGATCACAAGAAATTGAAAAGATCAACCGTAACTATCCCTATTTGGTTTGCGAATTTCAGCAGCCATAGTAATAGTTTCTTCAATAACATCAACAGCAATAACAACAGTGTGAATAGCATTCCAAGGCTTCATTTCAGAGATCACATAAGAACTTATAAGCAAGGTTTTAAAAAACGGGACGTTACCGCAAAAACGGCCGTTACAAATCGGTATCGGCAGTGGCCGTTACCGTTTTTGCCCCTTTTAACGCTGAGAAAGAAATTCACAGAAAAAACGCCGTTACGGCCGTTACGGAACGCCGTTACGACCGCGAAATGCCGTTACGCCCGCGAAACAGAAGAACAGGAGAAGAAGGACAGGCATCTCAAATCTCATATTAACAGGGGAAGAGGAATCTTATCTTTTGTACCAGCCAGGCATCTCAAATCTTTTGTACCACATCTCAAATCTTTTTTACACAAATCAATTACTTCTCTTCTTTGGTGGTGTGATTCTTTGTTCCTTCCTTCCTGCTTGCGATTTGCGATTCTGGTGTTCGTTCTGCTTGCGATTCTGGTGTGTGATTCTTTTGCGATTTGCGATTTGCGATTCTTTGTTCGTTCTGCTTGCGTCTCTTTGTTCGTTCTGCACTTCTGCTTGCGTCTCTCTGCTTGCCGTTCTGCACTTCTGCTTGCGTCTCCTAGGCAGGTTTGTGATAAGTGATAAGTATTTTTTGTTTTTTGAGCGAATAAGGTTTGTTATCACAATACACACACGTATGCACACACACAACTTCACTCTTCATTCAATAATAATCAATTAATAATAATAATAATAATAATAATAATAATAATAATAATAATTAATAATTACTTTTTTTTTGAAGGAAATTAATAATTACTTTTAGATGGTTTATTTGTTTATTCATATATTTATTTATTAAACTCAAAAAAAAAAAAGTGTGCATTCATCTCTTATTTAAGTTCAAGGTAATTTTAAATTGACACTCTTAATTTGAGTATTTAGGTAACAAATATTAGATGAAATTTTAGAGAAAAAATTGGGACTTTGATGTTAGATCATGAACTTTGTTTAAAGATATGTTATGAGTAGTTATTTATTAGTTAATTTCTTTTCAATCTGTCAAACACTCTATCTTTACTAAATGTAGTCTATTTTTATTATTATATTTACTTTAAACATGTTATAATTATGGAAAAAAAGTATTAATCTAAGTTATTATACATTATATGATAATATAAATAGTTTTAATACGTTTTGTGAAACGTTTTGATTAATTCACACCGCGAAGACCGTTATTTGTATCGCAGCGTCCGTTACAGCCGAAATCGCGACAACCTTAACGCGACCGCGATCGCGACCGCGACCGTTTTTTAGAACCTTGCTTATAAGCAAAGATATTTTGCTGCTGAAGCTGTTGAAGAAGCAGCAGAAGATAACTTCAACTACAATAATTGCGAAGCTGAAGAAGATGGTTCATGTGCTGATGGAATGAGACTAGTTCAACTTCTAATTGCATGTGCTGAAGCTGTCGCGTGTCGCGACAAATCACACGCTTCGGTTTTACTGTCCGAGCTTAAATCAAATGCTTTGGTGTTTGGTTCTTCATTTCAAAGAGTTGCTTCTTGTTTTGTTCAAGGACTCACAGAGAGACTAACTCTGATTCAACCAATTGGTAATAACTCTGCTGGATCAGATACAAAATCAATGATGAACATAATGGATGCTGCATCAGAAGAAATGGAAGAAGCATTCAAGTTGGTTTATGAAAATTGTCCACATATTCAATTTGGACATTTTGTGGCTAATTCCATAATATTGGAAGCCTTTGAGGGTGAGAGTTTTCTTCATGTGGTTGATCTTGGCATGAGCCTTGGTCTTCCACATGGACACCAATGGCGAGGACTAATTCAAAGTCTCGCGGATCGATCCAGCCACCGTGTTCGGCAGCTTAGGATCACAGCGATTGGTCTATGCATTGCTAGAATCCAAGTCATTGGTGAAGAACTCTCAATTTACGCGAAAAATTTGGGGATTCATTTGGAATTCTCAATTGTTGAGAAGAATTTGGAGAATTTGAAACCTAAGGACATAAAAGTGAATGAAAATGAGGTCCTTGTGGTTAACAGCATTCTTCAATTGCATTGTGTTGTTAAAGAGAGTCGTGGCGCTTTGAATGCGGTTTTACAAATGATCCACGGTCTTTCGCCAAAAGTGTTGGTTATGGCTGAGCAAGATTCTGGTCACAATGGACCTTTTTTCCTTGGAAGGTTTATGGAATCATTGCATTATTATTCTGCTATATTTGACTCATTGGATGCTATGCTGCCAAAGTATGACACTAAGAGGGCAAAAATGGAACAGTTTTATTTTGCTGAGGAGATAAAGAACATTGTGAGCTGTGAAGGACCATTGAGGATGGAGAGGCATGAGAAAGTTGATCAGTGGAGGAGGAGAATGAGTAGGGCTGGATTTCAAGGTTCACCTATTAAGATGGTTGTTCAAGCTAAGCAGTGGCTTGTGAAGAATAATGTTTGTGATGGATATACTGTTGTGGAAGAGAAAGGGTGTTTGGTTCTTGGATGGAAATCAAAGCCTATTGTTGCAGTTTCTTGCTGGAAATGCTGA
Protein sequence

>MS.gene057149.t1

MSPALYASTFKCEVDENPSSLMGSYYASLYPNLPILENSASSTWILNPFSDHETETTRDHKKLKRSTVTIPIWFANFSSHSNSFFNNINSNNNSVNSIPRLHFRDHIRTYKQGFKKRDVTAKTAVTNRYRQWPLPFLPLLTLRKKFTEKTPLRPLRNAVTTAKCRYARETEEQEKKDRHLKSHINRGRGILSFVPARHLKSFVPHLKSFLHKSITSLLWWCDSLFLPSCLRFAILVFVLLAILVCDSFAICDLRFFVRSACVSLFVLHFCLRLSACRSALLLASPRQNLAYKQRYFAAEAVEEAAEDNFNYNNCEAEEDGSCADGMRLVQLLIACAEAVACRDKSHASVLLSELKSNALVFGSSFQRVASCFVQGLTERLTLIQPIGNNSAGSDTKSMMNIMDAASEEMEEAFKLVYENCPHIQFGHFVANSIILEAFEGESFLHVVDLGMSLGLPHGHQWRGLIQSLADRSSHRVRQLRITAIGLCIARIQVIGEELSIYAKNLGIHLEFSIVEKNLENLKPKDIKVNENEVLVVNSILQLHCVVKESRGALNAVLQMIHGLSPKVLVMAEQDSGHNGPFFLGRFMESLHYYSAIFDSLDAMLPKYDTKRAKMEQFYFAEEIKNIVSCEGPLRMERHEKVDQWRRRMSRAGFQGSPIKMVVQAKQWLVKNNVCDGYTVVEEKGCLVLGWKSKPIVAVSCWKC