Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057181.t1 | RHN49639.1 | 68.2 | 157 | 27 | 4 | 31 | 165 | 12 | 167 | 1.60E-47 | 199.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057181.t1 | A0A396H8N5 | 68.2 | 157 | 27 | 4 | 31 | 165 | 12 | 167 | 1.1e-47 | 199.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene057181.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057181.t1 | MTR_7g117590 | 68.590 | 156 | 27 | 4 | 31 | 165 | 18 | 172 | 9.54e-65 | 196 |
| MS.gene057181.t1 | MTR_1g054470 | 57.500 | 160 | 35 | 6 | 1 | 154 | 1 | 133 | 6.02e-52 | 162 |
| MS.gene057181.t1 | MTR_2g012700 | 36.364 | 110 | 69 | 1 | 57 | 165 | 11 | 120 | 3.78e-27 | 99.0 |
| MS.gene057181.t1 | MTR_2g062330 | 38.462 | 117 | 67 | 3 | 50 | 165 | 27 | 139 | 1.10e-25 | 95.9 |
| MS.gene057181.t1 | MTR_5g071400 | 54.545 | 77 | 11 | 4 | 1 | 71 | 1 | 59 | 1.71e-16 | 70.9 |
| MS.gene057181.t1 | MTR_0010s0310 | 33.333 | 90 | 57 | 1 | 69 | 155 | 151 | 240 | 1.52e-13 | 66.6 |
| MS.gene057181.t1 | MTR_6g477950 | 36.275 | 102 | 61 | 3 | 64 | 164 | 46 | 144 | 5.10e-12 | 62.8 |
| MS.gene057181.t1 | MTR_6g087720 | 32.039 | 103 | 66 | 3 | 54 | 152 | 196 | 298 | 3.54e-11 | 60.5 |
| MS.gene057181.t1 | MTR_5g078270 | 31.461 | 89 | 58 | 1 | 70 | 155 | 210 | 298 | 5.32e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGATGGTTTGAGTTTGTT+AGG | 0.273672 | 8.1:+20831018 | MS.gene057181:CDS |
| CGAATTGTTGATGATGAAAC+TGG | 0.373851 | 8.1:+20830936 | MS.gene057181:CDS |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 8.1:+20830969 | MS.gene057181:CDS |
| GGCTGCTGGACGGAGAGTTC+TGG | 0.386406 | 8.1:+20830751 | MS.gene057181:CDS |
| TGGCCATCGACTTCTTCATT+TGG | 0.387700 | 8.1:-20830656 | None:intergenic |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 8.1:+20830976 | MS.gene057181:CDS |
| TCAGCGAAGAACGATGAAAA+TGG | 0.399065 | 8.1:+20830244 | None:intergenic |
| TTTGAAATTGTGCAAATACT+TGG | 0.402550 | 8.1:-20830676 | None:intergenic |
| ATTAGTTCTGCTACGGCTGC+TGG | 0.408831 | 8.1:+20830737 | MS.gene057181:CDS |
| CGAAGAAGAAGTTGAAGATC+GGG | 0.445939 | 8.1:+20830324 | MS.gene057181:CDS |
| ATGAAATGTGCGGCGGATGC+AGG | 0.449012 | 8.1:+20830273 | MS.gene057181:CDS |
| TGGATGGTTTGAGTTTGTTA+GGG | 0.484898 | 8.1:+20831019 | MS.gene057181:CDS |
| CTGCAGCGATGTAATTGATC+CGG | 0.509939 | 8.1:+20830601 | MS.gene057181:intron |
| CTTCTTCGTCCATCTCAAAA+CGG | 0.535091 | 8.1:-20830309 | None:intergenic |
| ACGAAGAAGAAGTTGAAGAT+CGG | 0.540825 | 8.1:+20830323 | MS.gene057181:CDS |
| CGATGTAATTGATCCGGAGT+TGG | 0.544205 | 8.1:+20830607 | MS.gene057181:CDS |
| CTTTGAATTGCAATCTTCCG+AGG | 0.557056 | 8.1:-20830888 | None:intergenic |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 8.1:+20830980 | MS.gene057181:CDS |
| GGTTTCAATTCGTCCAACTC+CGG | 0.567151 | 8.1:-20830620 | None:intergenic |
| AGAAATACGTTGGAAGTGGA+TGG | 0.575514 | 8.1:+20831003 | MS.gene057181:CDS |
| ATGGAGAAATACGTTGGAAG+TGG | 0.576439 | 8.1:+20830999 | MS.gene057181:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.580120 | 8.1:+20830730 | MS.gene057181:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.583531 | 8.1:+20830993 | MS.gene057181:CDS |
| CCATCTCAAAACGGCGACGA+TGG | 0.584489 | 8.1:-20830300 | None:intergenic |
| AGGGCAAAAGAACTCAAAGA+AGG | 0.584629 | 8.1:+20831038 | MS.gene057181:CDS |
| TTAATGTGTAGCGTATTCCT+CGG | 0.586314 | 8.1:+20830871 | MS.gene057181:intron |
| GCATCCGCCGCACATTTCAT+CGG | 0.590420 | 8.1:-20830270 | None:intergenic |
| GATACAAATAATTTGTGTGG+AGG | 0.593345 | 8.1:-20831089 | None:intergenic |
| ACTGATACAAATAATTTGTG+TGG | 0.607437 | 8.1:-20831092 | None:intergenic |
| GTTCTGCTACGGCTGCTGGA+CGG | 0.628877 | 8.1:+20830741 | MS.gene057181:CDS |
| AATCCAAATGAAGAAGTCGA+TGG | 0.671453 | 8.1:+20830653 | MS.gene057181:CDS |
| ATGGCGACCGATGAAATGTG+CGG | 0.726557 | 8.1:+20830263 | MS.gene057181:CDS |
| GCGACCGATGAAATGTGCGG+CGG | 0.753822 | 8.1:+20830266 | MS.gene057181:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAATTTCAAAATTTTCAAAA+CGG | + | chr8.1:20830689-20830708 | MS.gene057181:CDS | 15.0% |
| !!! | GTTTTTTATTTTATTTTTGC+TGG | + | chr8.1:20830795-20830814 | MS.gene057181:intron | 15.0% |
| !!! | TTGTTTTTTTTTTAATCATC+TGG | + | chr8.1:20830555-20830574 | MS.gene057181:intron | 15.0% |
| !! | TTAATCATCTGGTTATTTAT+TGG | + | chr8.1:20830566-20830585 | MS.gene057181:intron | 20.0% |
| ! | CTTAAAATAGCCTTCTAAAT+GGG | - | chr8.1:20830437-20830456 | None:intergenic | 25.0% |
| !! | ACTGATACAAATAATTTGTG+TGG | - | chr8.1:20831095-20831114 | None:intergenic | 25.0% |
| !! | TTTGAAATTGTGCAAATACT+TGG | - | chr8.1:20830679-20830698 | None:intergenic | 25.0% |
| !!! | CATTTAGAAGGCTATTTTAA+GGG | + | chr8.1:20830436-20830455 | MS.gene057181:intron | 25.0% |
| AAGTTCGTGATAAAGGAAAT+TGG | + | chr8.1:20830976-20830995 | MS.gene057181:CDS | 30.0% | |
| CCTTAAAATAGCCTTCTAAA+TGG | - | chr8.1:20830438-20830457 | None:intergenic | 30.0% | |
| ! | AAAACGGTGATCATACTTTT+TGG | + | chr8.1:20830705-20830724 | MS.gene057181:CDS | 30.0% |
| ! | GATACAAATAATTTGTGTGG+AGG | - | chr8.1:20831092-20831111 | None:intergenic | 30.0% |
| !! | CCATTTAGAAGGCTATTTTA+AGG | + | chr8.1:20830435-20830454 | MS.gene057181:intron | 30.0% |
| !!! | AAGATTGGGCTTTTTAGTTA+TGG | + | chr8.1:20830385-20830404 | MS.gene057181:intron | 30.0% |
| AATCCAAATGAAGAAGTCGA+TGG | + | chr8.1:20830653-20830672 | MS.gene057181:CDS | 35.0% | |
| AATTGGATGGAGAAATACGT+TGG | + | chr8.1:20830993-20831012 | MS.gene057181:CDS | 35.0% | |
| ACGAAGAAGAAGTTGAAGAT+CGG | + | chr8.1:20830323-20830342 | MS.gene057181:CDS | 35.0% | |
| ACGTCTGAGAAGAAAATTCT+AGG | + | chr8.1:20830483-20830502 | MS.gene057181:intron | 35.0% | |
| CAATCTTATTGCCGTCAAAA+CGG | - | chr8.1:20830372-20830391 | None:intergenic | 35.0% | |
| CGTCTGAGAAGAAAATTCTA+GGG | + | chr8.1:20830484-20830503 | MS.gene057181:intron | 35.0% | |
| GAATGTGAAGTTCGTGATAA+AGG | + | chr8.1:20830969-20830988 | MS.gene057181:CDS | 35.0% | |
| TCGTGATAAAGGAAATTGGA+TGG | + | chr8.1:20830980-20830999 | MS.gene057181:CDS | 35.0% | |
| TGAAAATGTCTTCTTCTAGC+AGG | + | chr8.1:20830515-20830534 | MS.gene057181:intron | 35.0% | |
| TTAAGGGTTGTTGTGAATCT+TGG | + | chr8.1:20830452-20830471 | MS.gene057181:intron | 35.0% | |
| TTAATGTGTAGCGTATTCCT+CGG | + | chr8.1:20830871-20830890 | MS.gene057181:intron | 35.0% | |
| ! | TCATTTGGATTTTCGTCTAC+AGG | - | chr8.1:20830644-20830663 | None:intergenic | 35.0% |
| !! | CGAATTGTTGATGATGAAAC+TGG | + | chr8.1:20830936-20830955 | MS.gene057181:CDS | 35.0% |
| !! | GCATAGTATTAGTTCTGCTA+CGG | + | chr8.1:20830730-20830749 | MS.gene057181:CDS | 35.0% |
| !! | GTTTTGACGGCAATAAGATT+GGG | + | chr8.1:20830371-20830390 | MS.gene057181:intron | 35.0% |
| !! | TGGATGGTTTGAGTTTGTTA+GGG | + | chr8.1:20831019-20831038 | MS.gene057181:CDS | 35.0% |
| AGAAATACGTTGGAAGTGGA+TGG | + | chr8.1:20831003-20831022 | MS.gene057181:CDS | 40.0% | |
| AGGGCAAAAGAACTCAAAGA+AGG | + | chr8.1:20831038-20831057 | MS.gene057181:CDS | 40.0% | |
| ATGGAGAAATACGTTGGAAG+TGG | + | chr8.1:20830999-20831018 | MS.gene057181:CDS | 40.0% | |
| CGAAGAAGAAGTTGAAGATC+GGG | + | chr8.1:20830324-20830343 | MS.gene057181:CDS | 40.0% | |
| CTTCTTCGTCCATCTCAAAA+CGG | - | chr8.1:20830312-20830331 | None:intergenic | 40.0% | |
| CTTTGAATTGCAATCTTCCG+AGG | - | chr8.1:20830891-20830910 | None:intergenic | 40.0% | |
| GAAGATCGGGTCATTTGATT+GGG | + | chr8.1:20830337-20830356 | MS.gene057181:intron | 40.0% | |
| TGAAGATCGGGTCATTTGAT+TGG | + | chr8.1:20830336-20830355 | MS.gene057181:intron | 40.0% | |
| TTATTGCCGTCAAAACGGTT+CGG | - | chr8.1:20830367-20830386 | None:intergenic | 40.0% | |
| ! | GTGGATGGTTTGAGTTTGTT+AGG | + | chr8.1:20831018-20831037 | MS.gene057181:CDS | 40.0% |
| ! | TTGCTGGAGTATTTGCACAT+GGG | + | chr8.1:20830811-20830830 | MS.gene057181:intron | 40.0% |
| ! | TTTGCTGGAGTATTTGCACA+TGG | + | chr8.1:20830810-20830829 | MS.gene057181:intron | 40.0% |
| !! | CGTTTTGACGGCAATAAGAT+TGG | + | chr8.1:20830370-20830389 | MS.gene057181:intron | 40.0% |
| !!! | TGGGCTTTTTAGTTATGGAC+CGG | + | chr8.1:20830390-20830409 | MS.gene057181:intron | 40.0% |
| ATGGACCGGGCAAAAATGAA+TGG | + | chr8.1:20830404-20830423 | MS.gene057181:intron | 45.0% | |
| CGATGTAATTGATCCGGAGT+TGG | + | chr8.1:20830607-20830626 | MS.gene057181:CDS | 45.0% | |
| CTGCAGCGATGTAATTGATC+CGG | + | chr8.1:20830601-20830620 | MS.gene057181:intron | 45.0% | |
| GGGCAAAAATGAATGGGTCA+CGG | + | chr8.1:20830411-20830430 | MS.gene057181:intron | 45.0% | |
| GGTTTCAATTCGTCCAACTC+CGG | - | chr8.1:20830623-20830642 | None:intergenic | 45.0% | |
| TGGACCGGGCAAAAATGAAT+GGG | + | chr8.1:20830405-20830424 | MS.gene057181:intron | 45.0% | |
| ! | GGTTTTCCGAACCGTTTTGA+CGG | + | chr8.1:20830358-20830377 | MS.gene057181:intron | 45.0% |
| !! | GTGACCCATTCATTTTTGCC+CGG | - | chr8.1:20830412-20830431 | None:intergenic | 45.0% |
| !! | TGGCCATCGACTTCTTCATT+TGG | - | chr8.1:20830659-20830678 | None:intergenic | 45.0% |
| !!! | GGGCTTTTTAGTTATGGACC+GGG | + | chr8.1:20830391-20830410 | MS.gene057181:intron | 45.0% |
| ATGGCGACCGATGAAATGTG+CGG | + | chr8.1:20830263-20830282 | MS.gene057181:CDS | 50.0% | |
| GAGTATTTGCACATGGGTGG+TGG | + | chr8.1:20830817-20830836 | MS.gene057181:intron | 50.0% | |
| ! | CTGGAGTATTTGCACATGGG+TGG | + | chr8.1:20830814-20830833 | MS.gene057181:intron | 50.0% |
| !! | ATTAGTTCTGCTACGGCTGC+TGG | + | chr8.1:20830737-20830756 | MS.gene057181:CDS | 50.0% |
| ATGAAATGTGCGGCGGATGC+AGG | + | chr8.1:20830273-20830292 | MS.gene057181:CDS | 55.0% | |
| CCATCTCAAAACGGCGACGA+TGG | - | chr8.1:20830303-20830322 | None:intergenic | 55.0% | |
| GCATCCGCCGCACATTTCAT+CGG | - | chr8.1:20830273-20830292 | None:intergenic | 55.0% | |
| TGGGTCACGGCCCATTTAGA+AGG | + | chr8.1:20830424-20830443 | MS.gene057181:intron | 55.0% | |
| !!! | CCATCGTCGCCGTTTTGAGA+TGG | + | chr8.1:20830300-20830319 | MS.gene057181:CDS | 55.0% |
| GCGACCGATGAAATGTGCGG+CGG | + | chr8.1:20830266-20830285 | MS.gene057181:CDS | 60.0% | |
| GTTCTGCTACGGCTGCTGGA+CGG | + | chr8.1:20830741-20830760 | MS.gene057181:CDS | 60.0% | |
| ! | GCACATGGGTGGTGGCTTTC+TGG | + | chr8.1:20830825-20830844 | MS.gene057181:intron | 60.0% |
| ! | GCTGGACGGAGAGTTCTGGT+TGG | + | chr8.1:20830755-20830774 | MS.gene057181:intron | 60.0% |
| GGCTGCTGGACGGAGAGTTC+TGG | + | chr8.1:20830751-20830770 | MS.gene057181:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 20830257 | 20831124 | 20830257 | ID=MS.gene057181 |
| chr8.1 | mRNA | 20830257 | 20831124 | 20830257 | ID=MS.gene057181.t1;Parent=MS.gene057181 |
| chr8.1 | exon | 20830257 | 20830345 | 20830257 | ID=MS.gene057181.t1.exon1;Parent=MS.gene057181.t1 |
| chr8.1 | CDS | 20830257 | 20830345 | 20830257 | ID=cds.MS.gene057181.t1;Parent=MS.gene057181.t1 |
| chr8.1 | exon | 20830607 | 20830772 | 20830607 | ID=MS.gene057181.t1.exon2;Parent=MS.gene057181.t1 |
| chr8.1 | CDS | 20830607 | 20830772 | 20830607 | ID=cds.MS.gene057181.t1;Parent=MS.gene057181.t1 |
| chr8.1 | exon | 20830882 | 20831124 | 20830882 | ID=MS.gene057181.t1.exon3;Parent=MS.gene057181.t1 |
| chr8.1 | CDS | 20830882 | 20831124 | 20830882 | ID=cds.MS.gene057181.t1;Parent=MS.gene057181.t1 |
| Gene Sequence |
| Protein sequence |
