AlfalfaGEDB | MS.gene057313

M. sativa cultivar XinJiangDaYe / MS.gene057313

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057313.t1	XP_003600854.2	82.9	216	37	0	1	216	1	216	5.00E-94	354

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057313.t1	Q8S9H7	50.0	80	33	1	92	171	129	201	7.7e-11	68.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057313.t1	G7JAP1	82.9	216	37	0	1	216	1	216	3.6e-94	354.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene057313.t1	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057313.t1	MTR_3g070110	92.130	216	17	0	1	216	1	216	1.51e-140	392
MS.gene057313.t1	MTR_5g081860	41.401	157	78	6	21	171	48	196	1.03e-20	87.8
MS.gene057313.t1	MTR_5g069710	37.209	129	68	2	49	171	80	201	2.25e-17	79.3
MS.gene057313.t1	MTR_5g088010	57.143	77	27	4	92	167	57	128	3.82e-17	77.8
MS.gene057313.t1	MTR_8g063600	33.735	166	94	6	9	167	17	173	8.74e-17	77.4
MS.gene057313.t1	MTR_3g462790	35.583	163	85	4	20	171	63	216	1.38e-16	77.0
MS.gene057313.t1	MTR_5g027570	36.879	141	78	4	38	174	32	165	1.64e-16	75.9
MS.gene057313.t1	MTR_7g067080	40.708	113	53	3	59	171	111	209	7.93e-16	75.1
MS.gene057313.t1	MTR_5g027550	43.023	86	43	2	61	140	37	122	1.24e-15	73.2
MS.gene057313.t1	MTR_5g088060	58.904	73	24	4	96	167	273	340	5.81e-15	73.2
MS.gene057313.t1	MTR_5g488160	58.904	73	24	4	96	167	273	340	5.81e-15	73.2
MS.gene057313.t1	MTR_3g104370	38.136	118	57	2	49	166	57	158	6.35e-15	71.2
MS.gene057313.t1	MTR_8g063870	39.007	141	64	6	32	170	42	162	6.44e-15	72.0
MS.gene057313.t1	MTR_1g048660	59.259	54	22	0	87	140	4	57	1.44e-14	67.0
MS.gene057313.t1	MTR_1g111830	32.639	144	79	3	27	170	67	192	1.37e-13	67.8
MS.gene057313.t1	MTR_4g107230	33.333	117	66	2	24	140	25	129	1.46e-13	68.6
MS.gene057313.t1	MTR_2g090305	46.667	75	33	2	94	168	27	94	1.46e-12	64.3
MS.gene057313.t1	MTR_2g100930	39.759	83	43	1	86	168	100	175	1.85e-12	65.9
MS.gene057313.t1	MTR_6g092540	37.692	130	57	6	83	212	87	192	2.79e-12	64.7
MS.gene057313.t1	MTR_4g100630	36.607	112	52	2	59	170	71	163	4.13e-12	64.7
MS.gene057313.t1	MTR_4g111975	44.304	79	37	2	92	170	135	206	5.43e-12	63.9
MS.gene057313.t1	MTR_5g037080	47.368	57	30	0	84	140	91	147	6.81e-12	63.9
MS.gene057313.t1	MTR_5g075760	45.833	96	43	5	64	158	77	164	9.02e-12	62.4
MS.gene057313.t1	MTR_4g015130	47.826	69	29	1	103	171	1	62	1.16e-11	59.3
MS.gene057313.t1	MTR_8g101650	44.000	75	34	1	92	166	102	168	1.74e-11	62.4
MS.gene057313.t1	MTR_0036s0260	45.333	75	34	2	94	168	27	94	4.21e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057313.t1	AT5G05790	41.406	128	63	3	49	172	82	201	5.21e-20	85.9
MS.gene057313.t1	AT5G05790	41.406	128	63	3	49	172	82	201	5.21e-20	85.9
MS.gene057313.t1	AT3G11280	38.217	157	79	7	21	172	54	197	4.13e-19	83.2
MS.gene057313.t1	AT3G11280	38.217	157	79	7	21	172	54	197	4.13e-19	83.2
MS.gene057313.t1	AT5G01200	45.299	117	50	4	56	171	112	215	2.83e-18	81.3
MS.gene057313.t1	AT2G38090	37.069	116	63	2	59	171	102	210	1.91e-16	76.6
MS.gene057313.t1	AT5G58900	44.681	94	45	1	78	171	124	210	4.51e-16	75.5
MS.gene057313.t1	AT5G08520	36.207	174	84	9	12	169	23	185	7.77e-16	74.7
MS.gene057313.t1	AT4G09450	39.837	123	61	4	70	186	66	181	3.23e-14	68.9
MS.gene057313.t1	AT5G04760	49.367	79	33	2	92	170	96	167	1.57e-13	67.4
MS.gene057313.t1	AT1G19000	50.877	57	28	0	84	140	91	147	5.15e-13	67.0
MS.gene057313.t1	AT1G19000	50.877	57	28	0	84	140	91	147	5.15e-13	67.0
MS.gene057313.t1	AT5G47390	35.878	131	66	3	86	216	87	199	6.82e-13	67.0
MS.gene057313.t1	AT1G70000	41.096	73	41	1	70	140	71	143	1.51e-12	65.5
MS.gene057313.t1	AT1G70000	41.096	73	41	1	70	140	71	143	1.51e-12	65.5
MS.gene057313.t1	AT3G10580	46.835	79	33	1	92	170	92	161	2.61e-12	64.7
MS.gene057313.t1	AT1G49010	45.570	79	36	2	92	170	131	202	3.04e-12	65.1
MS.gene057313.t1	AT3G10580	46.835	79	33	1	92	170	92	161	3.12e-12	64.7
MS.gene057313.t1	AT5G56840	52.632	57	27	0	92	148	88	144	2.60e-11	61.6
MS.gene057313.t1	AT5G61620	45.570	79	36	1	92	170	105	176	4.09e-11	61.6
MS.gene057313.t1	AT1G74840	55.102	49	22	0	92	140	93	141	6.35e-11	60.5
MS.gene057313.t1	AT1G74840	55.102	49	22	0	92	140	93	141	6.77e-11	60.8
MS.gene057313.t1	AT3G16350	48.148	54	28	0	87	140	129	182	9.02e-11	60.8

Find 30 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAACCAATAATCATCTTCT+TGG	0.216049	3.1:-57488027	None:intergenic
CATGATCTTTATCACATTCT+TGG	0.225450	3.1:-57488692	None:intergenic
ATTTCCAAGAAGATGATTAT+TGG	0.238910	3.1:+57488023	MS.gene057313:CDS
GACTCATATGCGTGGTTATT+TGG	0.243758	3.1:+57488609	MS.gene057313:CDS
ATAACTACTCTTGATCTTCT+TGG	0.251399	3.1:+57488579	MS.gene057313:CDS
AGTTCTATTGTTATTATTAT+CGG	0.281602	3.1:-57488194	None:intergenic
TTTGAGCATGGCTTGCTACT+TGG	0.356659	3.1:-57488476	None:intergenic
AAAATATCAAAAGGAAACTT+AGG	0.373972	3.1:+57488541	MS.gene057313:CDS
CATTCAAGAAAGTGGTTCAT+TGG	0.387227	3.1:+57488245	MS.gene057313:CDS
AGAATGGTAGAGAATATTCT+AGG	0.388611	3.1:+57487974	None:intergenic
TTGAGCATGGCTTGCTACTT+GGG	0.417939	3.1:-57488475	None:intergenic
TATTTGTAGGCGTTTCTTGA+AGG	0.450413	3.1:+57488383	MS.gene057313:intron
AAATATCAAAAGGAAACTTA+GGG	0.461487	3.1:+57488542	MS.gene057313:CDS
AGATGATTATTGGTTGATGA+AGG	0.467158	3.1:+57488033	MS.gene057313:CDS
AAAGTGGAAAGAAATCTCTA+AGG	0.472931	3.1:+57488428	MS.gene057313:CDS
AAAGAAATCATTCAAGAAAG+TGG	0.476326	3.1:+57488237	MS.gene057313:CDS
ATTTGTAGGCGTTTCTTGAA+GGG	0.494586	3.1:+57488384	MS.gene057313:intron
GATCAAGAGTAGTTATATCA+TGG	0.499404	3.1:-57488572	None:intergenic
TACACGGCAAAGACGGAAAG+TGG	0.504210	3.1:+57488412	MS.gene057313:CDS
GCATGGCTTGCTACTTGGGA+TGG	0.505418	3.1:-57488471	None:intergenic
TGTTGTCGTCTTCAATCACT+TGG	0.511632	3.1:-57488635	None:intergenic
GGACTTCTACACGGCAAAGA+CGG	0.559413	3.1:+57488405	MS.gene057313:CDS
ATACATATGAACATATCAAG+TGG	0.590413	3.1:-57488727	None:intergenic
TTCTTGAAGGGACTTCTACA+CGG	0.594579	3.1:+57488396	MS.gene057313:CDS
GAAGACGACAACAATAACAA+TGG	0.605151	3.1:+57488645	MS.gene057313:CDS
TCAGCACAACATCGATGAAG+TGG	0.609150	3.1:+57488087	MS.gene057313:CDS
TTATTATTATCGGTTGCATG+AGG	0.632701	3.1:-57488184	None:intergenic
GCACAACATCGATGAAGTGG+TGG	0.668571	3.1:+57488090	MS.gene057313:CDS
AATATCAAAAGGAAACTTAG+GGG	0.670754	3.1:+57488543	MS.gene057313:CDS
GAAGTGATGACTCATATGCG+TGG	0.764866	3.1:+57488601	MS.gene057313:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTCTATTGTTATTATTAT+CGG	-	chr3.1:57488197-57488216	None:intergenic	15.0%
!!	AAAATATCAAAAGGAAACTT+AGG	+	chr3.1:57488541-57488560	MS.gene057313:CDS	20.0%
!!	AAATATCAAAAGGAAACTTA+GGG	+	chr3.1:57488542-57488561	MS.gene057313:CDS	20.0%
!!	ATGATAGCAAAAATATCAAA+AGG	+	chr3.1:57488532-57488551	MS.gene057313:CDS	20.0%
!!!	TTTCTTGAATGATTTCTTTT+TGG	-	chr3.1:57488236-57488255	None:intergenic	20.0%
!	AAAGAAATCATTCAAGAAAG+TGG	+	chr3.1:57488237-57488256	MS.gene057313:CDS	25.0%
!	AATATCAAAAGGAAACTTAG+GGG	+	chr3.1:57488543-57488562	MS.gene057313:CDS	25.0%
!	ATTTCCAAGAAGATGATTAT+TGG	+	chr3.1:57488023-57488042	MS.gene057313:CDS	25.0%
!	GAAACAATAAACGATGAAAA+AGG	-	chr3.1:57488294-57488313	None:intergenic	25.0%
!!!	GACATGGATTTTTTATTTGT+AGG	+	chr3.1:57488370-57488389	MS.gene057313:intron	25.0%
	AAAGTGGAAAGAAATCTCTA+AGG	+	chr3.1:57488428-57488447	MS.gene057313:CDS	30.0%
	ATAACTACTCTTGATCTTCT+TGG	+	chr3.1:57488579-57488598	MS.gene057313:CDS	30.0%
	CATGATCTTTATCACATTCT+TGG	-	chr3.1:57488695-57488714	None:intergenic	30.0%
	GATCAAGAGTAGTTATATCA+TGG	-	chr3.1:57488575-57488594	None:intergenic	30.0%
	TCAACCAATAATCATCTTCT+TGG	-	chr3.1:57488030-57488049	None:intergenic	30.0%
!	TTATTATTATCGGTTGCATG+AGG	-	chr3.1:57488187-57488206	None:intergenic	30.0%
!!	AGATGATTATTGGTTGATGA+AGG	+	chr3.1:57488033-57488052	MS.gene057313:CDS	30.0%
!!	ATGCTCAAAAGTACCAAAAA+AGG	+	chr3.1:57488490-57488509	MS.gene057313:CDS	30.0%
!!!	TTTTTTGGTACTTTTGAGCA+TGG	-	chr3.1:57488491-57488510	None:intergenic	30.0%
	ATTTGTAGGCGTTTCTTGAA+GGG	+	chr3.1:57488384-57488403	MS.gene057313:intron	35.0%
	CATTCAAGAAAGTGGTTCAT+TGG	+	chr3.1:57488245-57488264	MS.gene057313:CDS	35.0%
	GAAGACGACAACAATAACAA+TGG	+	chr3.1:57488645-57488664	MS.gene057313:CDS	35.0%
	TATTTGTAGGCGTTTCTTGA+AGG	+	chr3.1:57488383-57488402	MS.gene057313:intron	35.0%
!!	GTCTTTGCTTTTGCCTTTTT+TGG	-	chr3.1:57488506-57488525	None:intergenic	35.0%
	GACTCATATGCGTGGTTATT+TGG	+	chr3.1:57488609-57488628	MS.gene057313:CDS	40.0%
	TGTTGTCGTCTTCAATCACT+TGG	-	chr3.1:57488638-57488657	None:intergenic	40.0%
	TTCTTGAAGGGACTTCTACA+CGG	+	chr3.1:57488396-57488415	MS.gene057313:CDS	40.0%
	GAAGTGATGACTCATATGCG+TGG	+	chr3.1:57488601-57488620	MS.gene057313:CDS	45.0%
	TCAGCACAACATCGATGAAG+TGG	+	chr3.1:57488087-57488106	MS.gene057313:CDS	45.0%
!!	TCGACGAGCGTTTCACTTTT+TGG	+	chr3.1:57488130-57488149	MS.gene057313:CDS	45.0%
!!	TTGAGCATGGCTTGCTACTT+GGG	-	chr3.1:57488478-57488497	None:intergenic	45.0%
!!	TTTGAGCATGGCTTGCTACT+TGG	-	chr3.1:57488479-57488498	None:intergenic	45.0%
	GCACAACATCGATGAAGTGG+TGG	+	chr3.1:57488090-57488109	MS.gene057313:CDS	50.0%
	GCGCACACATGTGTTTGACA+TGG	+	chr3.1:57488354-57488373	MS.gene057313:intron	50.0%
	GGACTTCTACACGGCAAAGA+CGG	+	chr3.1:57488405-57488424	MS.gene057313:CDS	50.0%
	TACACGGCAAAGACGGAAAG+TGG	+	chr3.1:57488412-57488431	MS.gene057313:CDS	50.0%
!!	GCATGGCTTGCTACTTGGGA+TGG	-	chr3.1:57488474-57488493	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	57487977	57488734	57487977	ID=MS.gene057313
chr3.1	mRNA	57487977	57488734	57487977	ID=MS.gene057313.t1;Parent=MS.gene057313
chr3.1	exon	57487977	57488284	57487977	ID=MS.gene057313.t1.exon1;Parent=MS.gene057313.t1
chr3.1	CDS	57487977	57488284	57487977	ID=cds.MS.gene057313.t1;Parent=MS.gene057313.t1
chr3.1	exon	57488392	57488734	57488392	ID=MS.gene057313.t1.exon2;Parent=MS.gene057313.t1
chr3.1	CDS	57488392	57488734	57488392	ID=cds.MS.gene057313.t1;Parent=MS.gene057313.t1

Gene Sequence

>MS.gene057313

ATGGTAGAGAATATTCTAGGTTACAGTCTTCATAGCATGATGAATAATTTCCAAGAAGATGATTATTGGTTGATGAAGGAAACTATGCTATGTAGTGTTGATCAAATCAATCAGCACAACATCGATGAAGTGGTGGTAGAAAATAATATTGCATCGACGAGCGTTTCACTTTTTGGATATGATTCAGTTGATTCTTTCAACTTCTCTCCTCATGCAACCGATAATAATAACAATAGAACTACGAATATCGTAGAATCCAAAAAGAAATCATTCAAGAAAGTGGTTCATTGGTCACGTGATGAACACATGTAAGTCCTTTTTCATCGTTTATTGTTTCATGTAGTTTGAATTATATCTCTCTTTATATATACTCGTGCGCGCACACATGTGTTTGACATGGATTTTTTATTTGTAGGCGTTTCTTGAAGGGACTTCTACACGGCAAAGACGGAAAGTGGAAAGAAATCTCTAAGGACTATGTAAAAACAAAGACTCCATCCCAAGTAGCAAGCCATGCTCAAAAGTACCAAAAAAGGCAAAAGCAAAGACTTGATGATGATAGCAAAAATATCAAAAGGAAACTTAGGGGTAGCATCCATGATATAACTACTCTTGATCTTCTTGGAAGTGATGACTCATATGCGTGGTTATTTGGTGACCAAGTGATTGAAGACGACAACAATAACAATGGATCACTCTCAGTCACAACTTTCAACCAAGAATGTGATAAAGATCATGAAGTTGTTGCTTCCACTTGA

Protein sequence

>MS.gene057313.t1

MVENILGYSLHSMMNNFQEDDYWLMKETMLCSVDQINQHNIDEVVVENNIASTSVSLFGYDSVDSFNFSPHATDNNNNRTTNIVESKKKSFKKVVHWSRDEHMRFLKGLLHGKDGKWKEISKDYVKTKTPSQVASHAQKYQKRQKQRLDDDSKNIKRKLRGSIHDITTLDLLGSDDSYAWLFGDQVIEDDNNNNGSLSVTTFNQECDKDHEVVAST