AlfalfaGEDB | MS.gene057467

M. sativa cultivar XinJiangDaYe / MS.gene057467

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057467.t1	AES77659.2	70.3	138	41	0	1	138	1	138	1.90E-46	195.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057467.t1	O04539	38.3	120	67	3	14	130	15	130	1.2e-12	74.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057467.t1	G7L5A9	70.3	138	41	0	1	138	1	138	1.4e-46	195.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene057467.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057467.t1	MTR_7g013380	74.803	127	32	0	12	138	98	224	1.36e-59	186
MS.gene057467.t1	MTR_7g013380	70.290	138	41	0	1	138	1	138	6.77e-55	174
MS.gene057467.t1	MTR_7g013380	71.765	85	24	0	6	90	178	262	2.02e-36	126
MS.gene057467.t1	MTR_8g062700	43.269	104	55	2	29	130	56	157	5.78e-19	82.4
MS.gene057467.t1	MTR_8g062700	43.269	104	55	2	29	130	56	157	5.95e-19	82.4
MS.gene057467.t1	MTR_3g006125	39.062	128	70	3	14	135	90	215	3.79e-18	78.2
MS.gene057467.t1	MTR_3g006105	35.938	128	74	3	14	135	9	134	2.09e-15	69.7
MS.gene057467.t1	MTR_5g036670	41.176	102	56	2	31	130	59	158	3.24e-15	71.6
MS.gene057467.t1	MTR_6g008995	36.752	117	70	2	14	130	38	150	6.11e-15	70.5
MS.gene057467.t1	MTR_3g006185	36.029	136	76	4	14	140	9	142	1.06e-14	67.4
MS.gene057467.t1	MTR_6g008970	37.815	119	68	4	14	130	64	178	4.80e-13	65.5
MS.gene057467.t1	MTR_1g104937	41.026	78	44	1	13	90	12	87	1.06e-12	60.8
MS.gene057467.t1	MTR_1g104940	43.590	78	42	2	13	90	14	89	1.64e-12	60.5
MS.gene057467.t1	MTR_3g006150	35.185	108	68	2	32	138	22	128	1.76e-12	61.2
MS.gene057467.t1	MTR_1g104960	33.065	124	77	4	14	135	14	133	1.77e-12	61.6
MS.gene057467.t1	MTR_3g006065	35.185	108	68	2	32	138	22	128	2.63e-12	61.2
MS.gene057467.t1	MTR_6g008995	37.234	94	57	1	37	130	2	93	4.68e-12	62.4
MS.gene057467.t1	MTR_1g105960	32.479	117	51	3	14	130	2	90	2.61e-11	57.0
MS.gene057467.t1	MTR_7g013410	30.357	112	71	3	17	128	2	106	4.04e-11	57.4
MS.gene057467.t1	MTR_1g105010	41.558	77	43	2	14	90	17	91	5.42e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057467.t1	AT1G70060	37.405	131	75	4	3	130	4	130	5.76e-16	73.9
MS.gene057467.t1	AT1G70060	37.405	131	75	4	3	130	4	130	5.76e-16	73.9
MS.gene057467.t1	AT1G24190	36.923	130	76	4	3	130	4	129	7.34e-16	73.6
MS.gene057467.t1	AT1G24190	36.923	130	76	4	3	130	4	129	7.78e-16	73.6
MS.gene057467.t1	AT1G24190	36.923	130	76	4	3	130	4	129	7.85e-16	73.6
MS.gene057467.t1	AT5G15020	41.026	117	65	4	14	130	53	165	4.93e-14	68.2
MS.gene057467.t1	AT5G15020	41.026	117	65	4	14	130	53	165	5.03e-14	68.2
MS.gene057467.t1	AT1G59890	37.607	117	69	3	14	130	46	158	8.55e-14	67.8
MS.gene057467.t1	AT1G59890	37.607	117	69	3	14	130	46	158	8.88e-14	67.4
MS.gene057467.t1	AT1G59890	37.607	117	69	3	14	130	46	158	9.15e-14	67.4
MS.gene057467.t1	AT1G59890	37.607	117	69	3	14	130	46	158	9.88e-14	67.4
MS.gene057467.t1	AT3G01320	39.496	119	65	4	14	130	58	171	1.35e-13	67.0
MS.gene057467.t1	AT1G70030	35.714	126	75	5	14	138	12	132	1.39e-13	64.3
MS.gene057467.t1	AT3G01320	39.496	119	65	4	14	130	58	171	1.41e-13	67.0
MS.gene057467.t1	AT1G10450	37.000	100	61	1	31	130	100	197	3.14e-12	63.2
MS.gene057467.t1	AT1G10450	37.000	100	61	1	31	130	49	146	3.91e-12	62.8
MS.gene057467.t1	AT1G10450	37.000	100	61	1	31	130	49	146	3.91e-12	62.8
MS.gene057467.t1	AT1G70030	33.051	118	76	3	14	130	23	138	1.71e-11	58.2
MS.gene057467.t1	AT1G27220	32.727	110	62	3	14	113	15	122	9.34e-11	57.4

Find 23 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTCATTGACAAATTCTT+TGG	0.276334	7.2:-83917321	None:intergenic
TAGGATTGATGCTGTGAATC+TGG	0.308646	7.2:+83917560	MS.gene057467:intron
ATAGATTTATTCAAGATAAC+AGG	0.341070	7.2:+83917745	MS.gene057467:CDS
GGAACATTGTGAAGCCAAAA+AGG	0.355327	7.2:+83917298	MS.gene057467:CDS
CGACTTTCTGAAAGTCTTGA+AGG	0.360292	7.2:+83917385	MS.gene057467:CDS
TGACTTTCTGAAAGTCTTGA+AGG	0.381266	7.2:+83917782	MS.gene057467:CDS
AACTTCAATGACAAATTCTT+CGG	0.410704	7.2:-83917718	None:intergenic
ATCGGAGGAGGAACAATCTC+TGG	0.440798	7.2:+83917674	MS.gene057467:CDS
AGCTTGATTTCATATCCATC+TGG	0.485942	7.2:-83917648	None:intergenic
GAATCTGGGTAAAATATTGA+AGG	0.487796	7.2:+83917575	MS.gene057467:CDS
ATATTGAAGGAATTATTTGA+AGG	0.515550	7.2:+83917588	MS.gene057467:CDS
AGAGATTGTTCCTCCTCCGA+TGG	0.550257	7.2:-83917672	None:intergenic
GACTTTCTGAAAGTCTTGAA+GGG	0.558807	7.2:+83917386	MS.gene057467:CDS
TCGAAATACTACATGCCAGA+TGG	0.575885	7.2:+83917633	MS.gene057467:CDS
AATCAAGCTTCCATCGGAGG+AGG	0.577026	7.2:+83917662	MS.gene057467:CDS
AGGATTGATGCTGTGAATCT+GGG	0.599560	7.2:+83917561	MS.gene057467:intron
GATTTATTCAAGATAACAGG+AGG	0.606846	7.2:+83917748	MS.gene057467:CDS
TGAAGGATTATATGACACAA+AGG	0.612910	7.2:+83917799	MS.gene057467:CDS
TGAAATCAAGCTTCCATCGG+AGG	0.622504	7.2:+83917659	MS.gene057467:CDS
GGGTAACCCGAGATAATGAA+AGG	0.627344	7.2:+83917266	None:intergenic
TGAAGGGTTACAAGACACAA+AGG	0.630499	7.2:+83917402	MS.gene057467:CDS
ATATGAAATCAAGCTTCCAT+CGG	0.662074	7.2:+83917656	MS.gene057467:CDS
ATTTATTCAAGATAACAGGA+GGG	0.684609	7.2:+83917749	MS.gene057467:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTATTATATTGTTAGA+TGG	+	chr7.2:83917453-83917472	MS.gene057467:intron	10.0%
!!	ATAGATTTATTCAAGATAAC+AGG	+	chr7.2:83917745-83917764	MS.gene057467:CDS	20.0%
!!	ATATTGAAGGAATTATTTGA+AGG	+	chr7.2:83917588-83917607	MS.gene057467:CDS	20.0%
!!!	ATCTAATTTATCTATCCTTT+TGG	+	chr7.2:83917500-83917519	MS.gene057467:intron	20.0%
!!!	TTTTTTTAATTCTCACAAGA+TGG	+	chr7.2:83917822-83917841	MS.gene057467:intron	20.0%
!	AACTTCAATGACAAATTCTT+CGG	-	chr7.2:83917721-83917740	None:intergenic	25.0%
!	AACTTCATTGACAAATTCTT+TGG	-	chr7.2:83917324-83917343	None:intergenic	25.0%
!	ATTTATTCAAGATAACAGGA+GGG	+	chr7.2:83917749-83917768	MS.gene057467:CDS	25.0%
	ATATGAAATCAAGCTTCCAT+CGG	+	chr7.2:83917656-83917675	MS.gene057467:CDS	30.0%
	ATCCTACAAAATGTACCAAA+AGG	-	chr7.2:83917518-83917537	None:intergenic	30.0%
	GAATCTGGGTAAAATATTGA+AGG	+	chr7.2:83917575-83917594	MS.gene057467:CDS	30.0%
	GATTTATTCAAGATAACAGG+AGG	+	chr7.2:83917748-83917767	MS.gene057467:CDS	30.0%
	TGAAGGATTATATGACACAA+AGG	+	chr7.2:83917799-83917818	MS.gene057467:CDS	30.0%
!	ATCCTTTTGGTACATTTTGT+AGG	+	chr7.2:83917513-83917532	MS.gene057467:intron	30.0%
!	TTTGTAGGATTCTATCCTTT+TGG	+	chr7.2:83917528-83917547	MS.gene057467:intron	30.0%
	AGCTTGATTTCATATCCATC+TGG	-	chr7.2:83917651-83917670	None:intergenic	35.0%
	CCTGAAGATCAATGACAAAA+TGG	-	chr7.2:83917886-83917905	None:intergenic	35.0%
!	CCATTTTGTCATTGATCTTC+AGG	+	chr7.2:83917883-83917902	MS.gene057467:intron	35.0%
!	GACAAATTCTTCGGCATTTT+TGG	-	chr7.2:83917712-83917731	None:intergenic	35.0%
!	GACTTTCTGAAAGTCTTGAA+GGG	+	chr7.2:83917386-83917405	MS.gene057467:CDS	35.0%
!	TGACTTTCTGAAAGTCTTGA+AGG	+	chr7.2:83917782-83917801	MS.gene057467:CDS	35.0%
!!	GACAAATTCTTTGGCCTTTT+TGG	-	chr7.2:83917315-83917334	None:intergenic	35.0%
	AGGATTGATGCTGTGAATCT+GGG	+	chr7.2:83917561-83917580	MS.gene057467:intron	40.0%
	GGAACATTGTGAAGCCAAAA+AGG	+	chr7.2:83917298-83917317	MS.gene057467:CDS	40.0%
	TAGGATTGATGCTGTGAATC+TGG	+	chr7.2:83917560-83917579	MS.gene057467:intron	40.0%
	TCGAAATACTACATGCCAGA+TGG	+	chr7.2:83917633-83917652	MS.gene057467:CDS	40.0%
	TGAAGGGTTACAAGACACAA+AGG	+	chr7.2:83917402-83917421	MS.gene057467:CDS	40.0%
	TTCTCACAAGATGGTGCAAA+AGG	+	chr7.2:83917831-83917850	MS.gene057467:intron	40.0%
	TTGTCATTGATCTTCAGGTG+TGG	+	chr7.2:83917888-83917907	MS.gene057467:intron	40.0%
!	CGACTTTCTGAAAGTCTTGA+AGG	+	chr7.2:83917385-83917404	MS.gene057467:CDS	40.0%
	CACAAGATGGTGCAAAAGGA+TGG	+	chr7.2:83917835-83917854	MS.gene057467:intron	45.0%
	TGAAATCAAGCTTCCATCGG+AGG	+	chr7.2:83917659-83917678	MS.gene057467:CDS	45.0%
	AATCAAGCTTCCATCGGAGG+AGG	+	chr7.2:83917662-83917681	MS.gene057467:CDS	50.0%
	AGAGATTGTTCCTCCTCCGA+TGG	-	chr7.2:83917675-83917694	None:intergenic	50.0%
	ATCGGAGGAGGAACAATCTC+TGG	+	chr7.2:83917674-83917693	MS.gene057467:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	83917281	83917926	83917281	ID=MS.gene057467
chr7.2	mRNA	83917281	83917926	83917281	ID=MS.gene057467.t1;Parent=MS.gene057467
chr7.2	exon	83917281	83917423	83917281	ID=MS.gene057467.t1.exon1;Parent=MS.gene057467.t1
chr7.2	CDS	83917281	83917423	83917281	ID=cds.MS.gene057467.t1;Parent=MS.gene057467.t1
chr7.2	exon	83917563	83917820	83917563	ID=MS.gene057467.t1.exon2;Parent=MS.gene057467.t1
chr7.2	CDS	83917563	83917820	83917563	ID=cds.MS.gene057467.t1;Parent=MS.gene057467.t1
chr7.2	exon	83917905	83917926	83917905	ID=MS.gene057467.t1.exon3;Parent=MS.gene057467.t1
chr7.2	CDS	83917905	83917926	83917905	ID=cds.MS.gene057467.t1;Parent=MS.gene057467.t1

Gene Sequence

>MS.gene057467

ATGAAAGGACATTTTCTGGAACATTGTGAAGCCAAAAAGGCCAAAGAATTTGTCAATGAAGTTAAGTGTAGATTTATTCAAGATAACATGAGAGATAAGTATGACGACTTTCTGAAAGTCTTGAAGGGTTACAAGACACAAAGGTTTGATCATGTGTGACTACTAATTTATTTTTTTTATTATATTGTTAGATGGATTTTATTAAATATTGTTGATTGTATCTAATTTATCTATCCTTTTGGTACATTTTGTAGGATTCTATCCTTTTGGTACATTTTGTAGGATTGATGCTGTGAATCTGGGTAAAATATTGAAGGAATTATTTGAAGGTCATAGAGATCTTATATCAAGATCGAAATACTACATGCCAGATGGATATGAAATCAAGCTTCCATCGGAGGAGGAACAATCTCTGGAACATTGCGAAGCCAAAAATGCCGAAGAATTTGTCATTGAAGTTAAACATAGATTTATTCAAGATAACAGGAGGGATATGTATGATGACTTTCTGAAAGTCTTGAAGGATTATATGACACAAAGGTTTTTTTAATTCTCACAAGATGGTGCAAAAGGATGGCATATTGCATATACTAAATGTTGTTCCATTTTGTCATTGATCTTCAGGTGTGGTGCAGCAAGATCTTAA

Protein sequence

>MS.gene057467.t1

MKGHFLEHCEAKKAKEFVNEVKCRFIQDNMRDKYDDFLKVLKGYKTQRIDAVNLGKILKELFEGHRDLISRSKYYMPDGYEIKLPSEEEQSLEHCEAKNAEEFVIEVKHRFIQDNRRDMYDDFLKVLKDYMTQRCGAARS