Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057671.t1 | XP_003622913.2 | 80.2 | 96 | 19 | 0 | 1 | 96 | 430 | 525 | 7.30E-33 | 150.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057671.t1 | Q9FYR7 | 43.8 | 105 | 50 | 2 | 1 | 96 | 403 | 507 | 1.7e-14 | 80.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057671.t1 | G7L175 | 80.2 | 96 | 19 | 0 | 1 | 96 | 430 | 525 | 5.3e-33 | 150.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene057671.t1 | TF | GRAS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057671.t1 | MTR_4g074310 | 69.231 | 117 | 23 | 2 | 1 | 117 | 114 | 217 | 2.45e-45 | 149 |
| MS.gene057671.t1 | MTR_7g057230 | 80.208 | 96 | 19 | 0 | 1 | 96 | 430 | 525 | 3.98e-44 | 155 |
| MS.gene057671.t1 | MTR_6g047750 | 48.913 | 92 | 44 | 1 | 2 | 90 | 431 | 522 | 1.97e-20 | 88.6 |
| MS.gene057671.t1 | MTR_3g089055 | 50.617 | 81 | 40 | 0 | 16 | 96 | 356 | 436 | 1.72e-15 | 73.9 |
| MS.gene057671.t1 | MTR_3g089055 | 50.617 | 81 | 40 | 0 | 16 | 96 | 351 | 431 | 1.87e-15 | 73.9 |
| MS.gene057671.t1 | MTR_1g029420 | 49.383 | 81 | 41 | 0 | 16 | 96 | 378 | 458 | 1.93e-14 | 70.9 |
| MS.gene057671.t1 | MTR_4g133660 | 43.210 | 81 | 46 | 0 | 16 | 96 | 329 | 409 | 2.65e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057671.t1 | AT5G52510 | 43.810 | 105 | 50 | 2 | 1 | 96 | 403 | 507 | 2.64e-18 | 82.4 |
| MS.gene057671.t1 | AT1G21450 | 41.975 | 81 | 47 | 0 | 16 | 96 | 378 | 458 | 5.90e-11 | 60.8 |
Find 37 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAATTTGATCGCTGGTTAT+TGG | 0.185580 | 4.4:-35033504 | None:intergenic |
| GAAAACCATCAGAGAACTTT+TGG | 0.210348 | 4.4:+35032752 | MS.gene057671:CDS |
| AGCTTCAAGGATTGCAATAT+TGG | 0.241145 | 4.4:+35033338 | MS.gene057671:intron |
| TTGCTTCAAGCTCTCTTTCA+TGG | 0.291733 | 4.4:-35033364 | None:intergenic |
| CGCGATTGGCTTTGGTTGGA+TGG | 0.310501 | 4.4:+35032976 | MS.gene057671:CDS |
| GGACGAGAAGCTGAAAGTTT+AGG | 0.344140 | 4.4:+35032774 | MS.gene057671:CDS |
| TCCGTTGACACGCTCTCATT+TGG | 0.351574 | 4.4:-35032915 | None:intergenic |
| AAGAACTCTCGCTGTTGATT+GGG | 0.353966 | 4.4:+35033000 | MS.gene057671:CDS |
| GAAATCCACGCGATTGGCTT+TGG | 0.364518 | 4.4:+35032968 | MS.gene057671:CDS |
| GAAGAACTCTCGCTGTTGAT+TGG | 0.379911 | 4.4:+35032999 | MS.gene057671:CDS |
| TATCTTCGAATGCAGCTTCA+AGG | 0.404851 | 4.4:+35033325 | MS.gene057671:intron |
| GAAATCCATGCAATTGGCTT+TGG | 0.417341 | 4.4:-35033042 | None:intergenic |
| TCACATGAAATCCATGCAAT+TGG | 0.428325 | 4.4:-35033048 | None:intergenic |
| GAAGCTGAAAGTTTAGGAAT+CGG | 0.429942 | 4.4:+35032780 | MS.gene057671:CDS |
| AAGCTGAAAGTTTAGGAATC+GGG | 0.438546 | 4.4:+35032781 | MS.gene057671:CDS |
| TGCAATTGGCTTTGGTTGGA+TGG | 0.439885 | 4.4:-35033034 | None:intergenic |
| TCCACGCGATTGGCTTTGGT+TGG | 0.466185 | 4.4:+35032972 | MS.gene057671:CDS |
| TCACATGAAATCCACGCGAT+TGG | 0.468333 | 4.4:+35032962 | MS.gene057671:CDS |
| TGAAAATCCACCTCCGGTAG+TGG | 0.474184 | 4.4:-35033441 | None:intergenic |
| TCCATGCAATTGGCTTTGGT+TGG | 0.483956 | 4.4:-35033038 | None:intergenic |
| TAAAACTGAAATTTGATCGC+TGG | 0.486089 | 4.4:-35033511 | None:intergenic |
| TCGTCCCAAAAGTTCTCTGA+TGG | 0.500146 | 4.4:-35032757 | None:intergenic |
| GCGATTGGCTTTGGTTGGAT+GGG | 0.517356 | 4.4:+35032977 | MS.gene057671:CDS |
| TTTGCTGAACATTCCACTAC+CGG | 0.524879 | 4.4:+35033428 | MS.gene057671:CDS |
| GAACATTCCACTACCGGAGG+TGG | 0.534778 | 4.4:+35033434 | MS.gene057671:CDS |
| GCAATTGGCTTTGGTTGGAT+GGG | 0.539085 | 4.4:-35033033 | None:intergenic |
| TCGCTGGTTATTGGATTGCA+TGG | 0.540291 | 4.4:-35033495 | None:intergenic |
| AATCGGGTTTGAATTCAAAC+CGG | 0.540939 | 4.4:+35032797 | MS.gene057671:CDS |
| GCCAAATGAGAGCGTGTCAA+CGG | 0.550607 | 4.4:+35032914 | MS.gene057671:CDS |
| AGAACTCTCGCTGTTGATTG+GGG | 0.565540 | 4.4:+35033001 | MS.gene057671:CDS |
| TAACAATGAAAATCCACCTC+CGG | 0.565897 | 4.4:-35033447 | None:intergenic |
| TCCAACCAAAGCCAATTGCA+TGG | 0.583125 | 4.4:+35033037 | MS.gene057671:CDS |
| TCCAACCAAAGCCAATCGCG+TGG | 0.639744 | 4.4:-35032973 | None:intergenic |
| TGACGAAGAAGCGTGTCGCG+TGG | 0.641458 | 4.4:-35032942 | None:intergenic |
| GTCATTGAATTGTGACTCAG+AGG | 0.645367 | 4.4:+35032851 | MS.gene057671:CDS |
| GCTGAACATTCCACTACCGG+AGG | 0.683216 | 4.4:+35033431 | MS.gene057671:CDS |
| GTTGAAGATCACGATTGTGA+CGG | 0.729260 | 4.4:+35032707 | MS.gene057671:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTATTGTGATTTTGATAT+TGG | - | chr4.4:35033283-35033302 | None:intergenic | 20.0% |
| !!! | TGTGATTTTGATATTGGTAA+TGG | - | chr4.4:35033277-35033296 | None:intergenic | 25.0% |
| AAAAAGAGTGATCAAGGAAA+AGG | + | chr4.4:35033385-35033404 | MS.gene057671:CDS | 30.0% | |
| TAAAACTGAAATTTGATCGC+TGG | - | chr4.4:35033514-35033533 | None:intergenic | 30.0% | |
| ! | AAAACCATCAGAGAACTTTT+GGG | + | chr4.4:35032753-35032772 | MS.gene057671:CDS | 30.0% |
| ! | AGCGATCAAATTTCAGTTTT+AGG | + | chr4.4:35033513-35033532 | MS.gene057671:CDS | 30.0% |
| ! | TCTCATTTGGCATTTTGTAT+AGG | - | chr4.4:35032905-35032924 | None:intergenic | 30.0% |
| !! | TTTTCATTGTTATTCACACG+CGG | + | chr4.4:35033458-35033477 | MS.gene057671:CDS | 30.0% |
| AAGCTGAAAGTTTAGGAATC+GGG | + | chr4.4:35032781-35032800 | MS.gene057671:CDS | 35.0% | |
| AATCGGGTTTGAATTCAAAC+CGG | + | chr4.4:35032797-35032816 | MS.gene057671:CDS | 35.0% | |
| AATCGGGTTTGAATTCAAAC+CGG | - | chr4.4:35032816-35032797 | None:intergenic | 35.0% | |
| AGCTTCAAGGATTGCAATAT+TGG | + | chr4.4:35033338-35033357 | MS.gene057671:intron | 35.0% | |
| GAAGCTGAAAGTTTAGGAAT+CGG | + | chr4.4:35032780-35032799 | MS.gene057671:CDS | 35.0% | |
| TAACAATGAAAATCCACCTC+CGG | - | chr4.4:35033450-35033469 | None:intergenic | 35.0% | |
| TCACATGAAATCCATGCAAT+TGG | - | chr4.4:35033051-35033070 | None:intergenic | 35.0% | |
| TCTCATTTGGCATTATGTAC+AGG | + | chr4.4:35033108-35033127 | MS.gene057671:intron | 35.0% | |
| TCTTCTAAGTCACAATTCAC+CGG | + | chr4.4:35033158-35033177 | MS.gene057671:intron | 35.0% | |
| TGAAGCAAAAAGAGTGATCA+AGG | + | chr4.4:35033379-35033398 | MS.gene057671:CDS | 35.0% | |
| ! | AAATCCATCGGAGAACTTTT+AGG | - | chr4.4:35033222-35033241 | None:intergenic | 35.0% |
| ! | GAAAACCATCAGAGAACTTT+TGG | + | chr4.4:35032752-35032771 | MS.gene057671:CDS | 35.0% |
| ! | GAAATTTGATCGCTGGTTAT+TGG | - | chr4.4:35033507-35033526 | None:intergenic | 35.0% |
| ! | TCCAAGAATCTGAAATCCAT+CGG | - | chr4.4:35033234-35033253 | None:intergenic | 35.0% |
| ! | TCTGATGGTTTTCAGATTCT+CGG | - | chr4.4:35032745-35032764 | None:intergenic | 35.0% |
| !! | TCTGTGAGTTTTATGTTCAC+CGG | - | chr4.4:35032819-35032838 | None:intergenic | 35.0% |
| AGAAGCTGAGTTTAGGAATC+GGG | - | chr4.4:35033196-35033215 | None:intergenic | 40.0% | |
| GAGAAGCTGAGTTTAGGAAT+CGG | - | chr4.4:35033197-35033216 | None:intergenic | 40.0% | |
| GGATTTCAGATTCTTGGAGA+AGG | + | chr4.4:35033236-35033255 | MS.gene057671:intron | 40.0% | |
| GTCATTGAATTGTGACTCAG+AGG | + | chr4.4:35032851-35032870 | MS.gene057671:CDS | 40.0% | |
| GTTGAAGATCACGATTGTGA+CGG | + | chr4.4:35032707-35032726 | MS.gene057671:CDS | 40.0% | |
| TATCTTCGAATGCAGCTTCA+AGG | + | chr4.4:35033325-35033344 | MS.gene057671:intron | 40.0% | |
| TCTATTGACACGCTCTCATT+TGG | + | chr4.4:35033095-35033114 | MS.gene057671:intron | 40.0% | |
| TTGCTTCAAGCTCTCTTTCA+TGG | - | chr4.4:35033367-35033386 | None:intergenic | 40.0% | |
| TTTGCTGAACATTCCACTAC+CGG | + | chr4.4:35033428-35033447 | MS.gene057671:CDS | 40.0% | |
| ! | AAGAACTCTCGCTGTTGATT+GGG | + | chr4.4:35033000-35033019 | MS.gene057671:CDS | 40.0% |
| ! | GAAATCCATGCAATTGGCTT+TGG | - | chr4.4:35033045-35033064 | None:intergenic | 40.0% |
| ! | TCCGATGGATTTCAGATTCT+TGG | + | chr4.4:35033230-35033249 | MS.gene057671:intron | 40.0% |
| GAAGAACTCTCGCTGTTGAT+TGG | + | chr4.4:35032999-35033018 | MS.gene057671:CDS | 45.0% | |
| GGACGAGAAGCTGAAAGTTT+AGG | + | chr4.4:35032774-35032793 | MS.gene057671:CDS | 45.0% | |
| TAGGTCGAGAAGCTGAGTTT+AGG | - | chr4.4:35033203-35033222 | None:intergenic | 45.0% | |
| TCACATGAAATCCACGCGAT+TGG | + | chr4.4:35032962-35032981 | MS.gene057671:CDS | 45.0% | |
| TCCAACCAAAGCCAATTGCA+TGG | + | chr4.4:35033037-35033056 | MS.gene057671:CDS | 45.0% | |
| TCGACCTAAAAGTTCTCCGA+TGG | + | chr4.4:35033215-35033234 | MS.gene057671:intron | 45.0% | |
| TCGTCCCAAAAGTTCTCTGA+TGG | - | chr4.4:35032760-35032779 | None:intergenic | 45.0% | |
| ! | AGAACTCTCGCTGTTGATTG+GGG | + | chr4.4:35033001-35033020 | MS.gene057671:CDS | 45.0% |
| ! | TCGCTGGTTATTGGATTGCA+TGG | - | chr4.4:35033498-35033517 | None:intergenic | 45.0% |
| !! | GCAATTGGCTTTGGTTGGAT+GGG | - | chr4.4:35033036-35033055 | None:intergenic | 45.0% |
| !! | TCCATGCAATTGGCTTTGGT+TGG | - | chr4.4:35033041-35033060 | None:intergenic | 45.0% |
| !! | TGCAATTGGCTTTGGTTGGA+TGG | - | chr4.4:35033037-35033056 | None:intergenic | 45.0% |
| TCCGTTGACACGCTCTCATT+TGG | - | chr4.4:35032918-35032937 | None:intergenic | 50.0% | |
| TGAAAATCCACCTCCGGTAG+TGG | - | chr4.4:35033444-35033463 | None:intergenic | 50.0% | |
| ! | GCCAAATGAGAGCGTGTCAA+CGG | + | chr4.4:35032914-35032933 | MS.gene057671:CDS | 50.0% |
| !! | GAAATCCACGCGATTGGCTT+TGG | + | chr4.4:35032968-35032987 | MS.gene057671:CDS | 50.0% |
| !! | GCGATTGGCTTTGGTTGGAT+GGG | + | chr4.4:35032977-35032996 | MS.gene057671:CDS | 50.0% |
| GAACATTCCACTACCGGAGG+TGG | + | chr4.4:35033434-35033453 | MS.gene057671:CDS | 55.0% | |
| GCTGAACATTCCACTACCGG+AGG | + | chr4.4:35033431-35033450 | MS.gene057671:CDS | 55.0% | |
| TCCAACCAAAGCCAATCGCG+TGG | - | chr4.4:35032976-35032995 | None:intergenic | 55.0% | |
| ! | TGACGAAGAAGCGTGTCTCG+TGG | + | chr4.4:35033068-35033087 | MS.gene057671:intron | 55.0% |
| !! | CGCGATTGGCTTTGGTTGGA+TGG | + | chr4.4:35032976-35032995 | MS.gene057671:CDS | 55.0% |
| !! | TCCACGCGATTGGCTTTGGT+TGG | + | chr4.4:35032972-35032991 | MS.gene057671:CDS | 55.0% |
| ! | TGACGAAGAAGCGTGTCGCG+TGG | - | chr4.4:35032945-35032964 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 35032705 | 35033547 | 35032705 | ID=MS.gene057671 |
| chr4.4 | mRNA | 35032705 | 35033547 | 35032705 | ID=MS.gene057671.t1;Parent=MS.gene057671 |
| chr4.4 | exon | 35032705 | 35033066 | 35032705 | ID=MS.gene057671.t1.exon1;Parent=MS.gene057671.t1 |
| chr4.4 | CDS | 35032705 | 35033066 | 35032705 | ID=cds.MS.gene057671.t1;Parent=MS.gene057671.t1 |
| chr4.4 | exon | 35033340 | 35033547 | 35033340 | ID=MS.gene057671.t1.exon2;Parent=MS.gene057671.t1 |
| chr4.4 | CDS | 35033340 | 35033547 | 35033340 | ID=cds.MS.gene057671.t1;Parent=MS.gene057671.t1 |
| Gene Sequence |
| Protein sequence |
