Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057678.t1 | RHN79076.1 | 76.3 | 257 | 46 | 4 | 1 | 243 | 1 | 256 | 7.80E-68 | 267.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057678.t1 | A0A396JLE5 | 76.3 | 257 | 46 | 4 | 1 | 243 | 1 | 256 | 5.6e-68 | 267.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene057678.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057678.t1 | MTR_4g073310 | 71.942 | 278 | 25 | 3 | 1 | 276 | 1 | 227 | 7.01e-117 | 335 |
| MS.gene057678.t1 | MTR_1g052185 | 45.794 | 321 | 79 | 13 | 1 | 272 | 1 | 275 | 4.78e-60 | 192 |
| MS.gene057678.t1 | MTR_1g046810 | 35.197 | 304 | 133 | 12 | 20 | 276 | 28 | 314 | 9.24e-33 | 122 |
| MS.gene057678.t1 | MTR_1g053530 | 34.906 | 212 | 113 | 7 | 23 | 233 | 25 | 212 | 2.56e-21 | 90.5 |
| MS.gene057678.t1 | MTR_5g098470 | 29.289 | 239 | 123 | 10 | 6 | 219 | 40 | 257 | 2.15e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057678.t1 | AT2G45120 | 30.233 | 129 | 79 | 1 | 19 | 136 | 180 | 308 | 3.80e-11 | 62.8 |
Find 49 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGATCATGAACATCTTAA+TGG | 0.235891 | 4.4:-35110793 | MS.gene057678:CDS |
| AGAAGAACATGGATTATAAA+AGG | 0.308077 | 4.4:+35111200 | None:intergenic |
| CCGCTCTCATACTATTAATA+AGG | 0.314029 | 4.4:-35111061 | MS.gene057678:CDS |
| AAATTCATAACAACTCCTTT+TGG | 0.331715 | 4.4:+35110463 | None:intergenic |
| CCTCCTGATAGAACGTTTGT+TGG | 0.361348 | 4.4:+35110825 | None:intergenic |
| TGCGCTGCGGCTGGTGGTGG+TGG | 0.387987 | 4.4:-35110745 | MS.gene057678:CDS |
| AAACCAACAAACGTTCTATC+AGG | 0.407703 | 4.4:-35110828 | MS.gene057678:CDS |
| GCTGGTGGTGGTGGTGATCA+TGG | 0.417182 | 4.4:-35110736 | MS.gene057678:CDS |
| GGACTTTGGTGGCAAATACT+TGG | 0.426874 | 4.4:+35110855 | None:intergenic |
| AACTCACACTCATACATTCT+CGG | 0.428608 | 4.4:+35111141 | None:intergenic |
| ATGTCCGCGAAAAGCCTTGT+TGG | 0.435044 | 4.4:+35110984 | None:intergenic |
| TATGATATTGCACCGTGAAA+AGG | 0.448967 | 4.4:-35110962 | MS.gene057678:CDS |
| TCTTGCGCTGCGGCTGGTGG+TGG | 0.459861 | 4.4:-35110748 | MS.gene057678:CDS |
| TTGTCCTTCACCTTGGTCGC+CGG | 0.460363 | 4.4:+35110631 | None:intergenic |
| ATGATATTGCACCGTGAAAA+GGG | 0.472390 | 4.4:-35110961 | MS.gene057678:CDS |
| GGTTGACGATTGAATACTCT+TGG | 0.485743 | 4.4:+35110936 | None:intergenic |
| TCTAATGAATTGAAGCCAAA+AGG | 0.489056 | 4.4:-35110478 | MS.gene057678:CDS |
| TTGAAATTGTTCCAAGGATG+TGG | 0.492696 | 4.4:+35110915 | None:intergenic |
| AATATGTCTTGCGCTGCGGC+TGG | 0.494836 | 4.4:-35110754 | MS.gene057678:CDS |
| TCAATCGTCAACCACATCCT+TGG | 0.496081 | 4.4:-35110926 | MS.gene057678:CDS |
| GAACAATTTCAATTTCAAGT+TGG | 0.498276 | 4.4:-35110904 | MS.gene057678:CDS |
| AAATACTTGGTTAAGTCAAT+CGG | 0.503127 | 4.4:+35110868 | None:intergenic |
| TCATGTGCAACATCTTCTGT+TGG | 0.510423 | 4.4:+35110424 | None:intergenic |
| TTCAACTCTGGCAACGCACT+CGG | 0.512404 | 4.4:-35111105 | MS.gene057678:CDS |
| TTGAAATTGAAATTGTTCCA+AGG | 0.519609 | 4.4:+35110909 | None:intergenic |
| ATTATTGTTGTCCTTCACCT+TGG | 0.532929 | 4.4:+35110624 | None:intergenic |
| CTTGCACTCATGAGGAAGAA+AGG | 0.539075 | 4.4:-35111237 | None:intergenic |
| ATGTCTTGCGCTGCGGCTGG+TGG | 0.545212 | 4.4:-35110751 | MS.gene057678:CDS |
| TGATGAAACACCGGCGACCA+AGG | 0.566718 | 4.4:-35110641 | MS.gene057678:CDS |
| GACAAGATCGAGAAACAAGT+TGG | 0.568686 | 4.4:-35110670 | MS.gene057678:CDS |
| TTGGTTAAGTCAATCGGTGG+TGG | 0.571109 | 4.4:+35110874 | None:intergenic |
| AAGGTCTCGCAATGCAGCCA+AGG | 0.578454 | 4.4:-35110553 | MS.gene057678:CDS |
| AGTGTGAGTTGTGCGGCAAA+AGG | 0.579769 | 4.4:-35111128 | MS.gene057678:CDS |
| TGCGGCAAAAGGTTCAACTC+TGG | 0.586813 | 4.4:-35111117 | MS.gene057678:CDS |
| TAGTTGTCCGATTTGCAACA+AGG | 0.592837 | 4.4:-35111016 | MS.gene057678:CDS |
| AACTCTGGCAACGCACTCGG+TGG | 0.616672 | 4.4:-35111102 | MS.gene057678:CDS |
| CTTCAATATGTCTTGCGCTG+CGG | 0.618324 | 4.4:-35110758 | MS.gene057678:CDS |
| GGATTATAAAAGGAACAAGA+AGG | 0.636811 | 4.4:+35111210 | None:intergenic |
| AATGAATGAAAAGAAGAACA+TGG | 0.641497 | 4.4:+35111189 | None:intergenic |
| AACACCGGCGACCAAGGTGA+AGG | 0.642622 | 4.4:-35110635 | MS.gene057678:CDS |
| TGAAGGTGGTGAAACTGCGA+TGG | 0.648342 | 4.4:-35110509 | MS.gene057678:CDS |
| TACTTGGTTAAGTCAATCGG+TGG | 0.654609 | 4.4:+35110871 | None:intergenic |
| CCAACAAACGTTCTATCAGG+AGG | 0.665230 | 4.4:-35110825 | MS.gene057678:CDS |
| TGGTGACATTGATGAAACAC+CGG | 0.668270 | 4.4:-35110650 | MS.gene057678:CDS |
| GCTCAAAGTTTCAAGCAATG+TGG | 0.669423 | 4.4:-35110698 | MS.gene057678:CDS |
| ATTCAAGATTAAAGACAACA+AGG | 0.676790 | 4.4:-35110572 | MS.gene057678:CDS |
| TATTAATAGTATGAGAGCGG+CGG | 0.687350 | 4.4:+35111064 | None:intergenic |
| CCTTATTAATAGTATGAGAG+CGG | 0.698742 | 4.4:+35111061 | None:intergenic |
| ATGTATGAGTGTGAGTTGTG+CGG | 0.703631 | 4.4:-35111135 | MS.gene057678:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAGAAGAAAAATAATGA+AGG | - | chr4.4:35111122-35111141 | MS.gene057678:CDS | 20.0% |
| ! | AAATACTTGGTTAAGTCAAT+CGG | + | chr4.4:35110783-35110802 | None:intergenic | 25.0% |
| ! | AAGAAGAAAAATAATGAAGG+TGG | - | chr4.4:35111125-35111144 | MS.gene057678:CDS | 25.0% |
| ! | AATGAATGAAAAGAAGAACA+TGG | + | chr4.4:35110462-35110481 | None:intergenic | 25.0% |
| ! | ATTCAAGATTAAAGACAACA+AGG | - | chr4.4:35111076-35111095 | MS.gene057678:CDS | 25.0% |
| ! | ATTGATCATGAACATCTTAA+TGG | - | chr4.4:35110855-35110874 | MS.gene057678:CDS | 25.0% |
| ! | TTGAAATTGAAATTGTTCCA+AGG | + | chr4.4:35110742-35110761 | None:intergenic | 25.0% |
| !! | AAATTCATAACAACTCCTTT+TGG | + | chr4.4:35111188-35111207 | None:intergenic | 25.0% |
| !! | AGAAGAACATGGATTATAAA+AGG | + | chr4.4:35110451-35110470 | None:intergenic | 25.0% |
| !! | GAACAATTTCAATTTCAAGT+TGG | - | chr4.4:35110744-35110763 | MS.gene057678:CDS | 25.0% |
| CCTTATTAATAGTATGAGAG+CGG | + | chr4.4:35110590-35110609 | None:intergenic | 30.0% | |
| GAAAAAACCTTGTTGCAAAT+CGG | + | chr4.4:35110642-35110661 | None:intergenic | 30.0% | |
| GGATTATAAAAGGAACAAGA+AGG | + | chr4.4:35110441-35110460 | None:intergenic | 30.0% | |
| TCTAATGAATTGAAGCCAAA+AGG | - | chr4.4:35111170-35111189 | MS.gene057678:CDS | 30.0% | |
| ! | TTTTCTTCTTTTCTTCACCT+TGG | + | chr4.4:35111115-35111134 | None:intergenic | 30.0% |
| !!! | AGAACGTTTGTTGGTTTTAT+GGG | + | chr4.4:35110817-35110836 | None:intergenic | 30.0% |
| !!! | TAGAACGTTTGTTGGTTTTA+TGG | + | chr4.4:35110818-35110837 | None:intergenic | 30.0% |
| AAACCAACAAACGTTCTATC+AGG | - | chr4.4:35110820-35110839 | MS.gene057678:CDS | 35.0% | |
| AACTCACACTCATACATTCT+CGG | + | chr4.4:35110510-35110529 | None:intergenic | 35.0% | |
| ATGATATTGCACCGTGAAAA+GGG | - | chr4.4:35110687-35110706 | MS.gene057678:CDS | 35.0% | |
| ATTATTGTTGTCCTTCACCT+TGG | + | chr4.4:35111027-35111046 | None:intergenic | 35.0% | |
| CCGCTCTCATACTATTAATA+AGG | - | chr4.4:35110587-35110606 | MS.gene057678:CDS | 35.0% | |
| TATGATATTGCACCGTGAAA+AGG | - | chr4.4:35110686-35110705 | MS.gene057678:CDS | 35.0% | |
| TATTAATAGTATGAGAGCGG+CGG | + | chr4.4:35110587-35110606 | None:intergenic | 35.0% | |
| TTGAAATTGTTCCAAGGATG+TGG | + | chr4.4:35110736-35110755 | None:intergenic | 35.0% | |
| !!! | CAAGGTTTTTTCATCCAACA+AGG | - | chr4.4:35110650-35110669 | MS.gene057678:CDS | 35.0% |
| !!! | TTGTTGGTTTTATGGGACTT+TGG | + | chr4.4:35110810-35110829 | None:intergenic | 35.0% |
| ATGTATGAGTGTGAGTTGTG+CGG | - | chr4.4:35110513-35110532 | MS.gene057678:CDS | 40.0% | |
| GACAAGATCGAGAAACAAGT+TGG | - | chr4.4:35110978-35110997 | MS.gene057678:CDS | 40.0% | |
| GCTCAAAGTTTCAAGCAATG+TGG | - | chr4.4:35110950-35110969 | MS.gene057678:CDS | 40.0% | |
| GGTTGACGATTGAATACTCT+TGG | + | chr4.4:35110715-35110734 | None:intergenic | 40.0% | |
| TACTTGGTTAAGTCAATCGG+TGG | + | chr4.4:35110780-35110799 | None:intergenic | 40.0% | |
| TCATGTGCAACATCTTCTGT+TGG | + | chr4.4:35111227-35111246 | None:intergenic | 40.0% | |
| TGGTGACATTGATGAAACAC+CGG | - | chr4.4:35110998-35111017 | MS.gene057678:CDS | 40.0% | |
| ! | TACTCTTGGAACCCTTTTCA+CGG | + | chr4.4:35110701-35110720 | None:intergenic | 40.0% |
| ! | TAGTTGTCCGATTTGCAACA+AGG | - | chr4.4:35110632-35110651 | MS.gene057678:CDS | 40.0% |
| !!! | TTGGTTTTATGGGACTTTGG+TGG | + | chr4.4:35110807-35110826 | None:intergenic | 40.0% |
| CCAACAAACGTTCTATCAGG+AGG | - | chr4.4:35110823-35110842 | MS.gene057678:CDS | 45.0% | |
| CCTCCTGATAGAACGTTTGT+TGG | + | chr4.4:35110826-35110845 | None:intergenic | 45.0% | |
| CTTCAATATGTCTTGCGCTG+CGG | - | chr4.4:35110890-35110909 | MS.gene057678:CDS | 45.0% | |
| TCAATCGTCAACCACATCCT+TGG | - | chr4.4:35110722-35110741 | MS.gene057678:CDS | 45.0% | |
| TTGGTTAAGTCAATCGGTGG+TGG | + | chr4.4:35110777-35110796 | None:intergenic | 45.0% | |
| ! | GGACTTTGGTGGCAAATACT+TGG | + | chr4.4:35110796-35110815 | None:intergenic | 45.0% |
| ! | TCATCCAACAAGGCTTTTCG+CGG | - | chr4.4:35110660-35110679 | MS.gene057678:CDS | 45.0% |
| AGTGTGAGTTGTGCGGCAAA+AGG | - | chr4.4:35110520-35110539 | MS.gene057678:CDS | 50.0% | |
| TGAAGGTGGTGAAACTGCGA+TGG | - | chr4.4:35111139-35111158 | MS.gene057678:CDS | 50.0% | |
| TGCGGCAAAAGGTTCAACTC+TGG | - | chr4.4:35110531-35110550 | MS.gene057678:CDS | 50.0% | |
| TTCAACTCTGGCAACGCACT+CGG | - | chr4.4:35110543-35110562 | MS.gene057678:CDS | 50.0% | |
| ! | ATGTCCGCGAAAAGCCTTGT+TGG | + | chr4.4:35110667-35110686 | None:intergenic | 50.0% |
| AAGGTCTCGCAATGCAGCCA+AGG | - | chr4.4:35111095-35111114 | MS.gene057678:CDS | 55.0% | |
| AATATGTCTTGCGCTGCGGC+TGG | - | chr4.4:35110894-35110913 | MS.gene057678:CDS | 55.0% | |
| TGATGAAACACCGGCGACCA+AGG | - | chr4.4:35111007-35111026 | MS.gene057678:CDS | 55.0% | |
| TTGTCCTTCACCTTGGTCGC+CGG | + | chr4.4:35111020-35111039 | None:intergenic | 55.0% | |
| ! | AGCGGCGGTGAGATGTTTTG+TGG | + | chr4.4:35110572-35110591 | None:intergenic | 55.0% |
| AACACCGGCGACCAAGGTGA+AGG | - | chr4.4:35111013-35111032 | MS.gene057678:CDS | 60.0% | |
| AACTCTGGCAACGCACTCGG+TGG | - | chr4.4:35110546-35110565 | MS.gene057678:CDS | 60.0% | |
| !! | GCTGGTGGTGGTGGTGATCA+TGG | - | chr4.4:35110912-35110931 | MS.gene057678:CDS | 60.0% |
| ATGTCTTGCGCTGCGGCTGG+TGG | - | chr4.4:35110897-35110916 | MS.gene057678:CDS | 65.0% | |
| TCTTGCGCTGCGGCTGGTGG+TGG | - | chr4.4:35110900-35110919 | MS.gene057678:CDS | 70.0% | |
| !! | TGCGCTGCGGCTGGTGGTGG+TGG | - | chr4.4:35110903-35110922 | MS.gene057678:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 35110420 | 35111250 | 35110420 | ID=MS.gene057678 |
| chr4.4 | mRNA | 35110420 | 35111250 | 35110420 | ID=MS.gene057678.t1;Parent=MS.gene057678 |
| chr4.4 | exon | 35110420 | 35111250 | 35110420 | ID=MS.gene057678.t1.exon1;Parent=MS.gene057678.t1 |
| chr4.4 | CDS | 35110420 | 35111250 | 35110420 | ID=cds.MS.gene057678.t1;Parent=MS.gene057678.t1 |
| Gene Sequence |
| Protein sequence |
