AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057681

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057681.t1 MTR_6g011450 84.556 518 75 3 29 541 309 826 0.0 837
MS.gene057681.t1 MTR_6g011450 84.556 518 75 3 29 541 171 688 0.0 835
MS.gene057681.t1 MTR_4g073340 81.365 542 65 4 1 541 1 507 0.0 800
MS.gene057681.t1 MTR_8g086050 86.142 267 15 3 547 800 367 624 9.00e-157 471
MS.gene057681.t1 MTR_8g086050 86.466 266 14 3 547 799 367 623 2.78e-156 473
MS.gene057681.t1 MTR_8g085680 75.564 266 43 3 547 799 366 622 9.15e-137 422
MS.gene057681.t1 MTR_8g085680 75.564 266 43 3 547 799 366 622 1.48e-136 422
MS.gene057681.t1 MTR_8g086050 85.470 234 12 3 547 767 367 591 1.26e-131 406
MS.gene057681.t1 MTR_6g011470 71.837 245 33 3 285 528 1 210 2.45e-112 340
MS.gene057681.t1 MTR_8g085680 76.630 184 30 1 547 717 366 549 2.21e-90 297
MS.gene057681.t1 MTR_2g056120 53.933 267 101 4 547 800 363 620 1.03e-88 296
MS.gene057681.t1 MTR_7g103840 33.526 173 110 3 362 531 846 1016 5.26e-30 128
MS.gene057681.t1 MTR_7g103840 33.526 173 110 3 362 531 848 1018 5.68e-30 128
MS.gene057681.t1 MTR_1g061130 34.104 173 107 4 362 529 839 1009 1.95e-28 123
MS.gene057681.t1 MTR_1g061130 34.104 173 107 4 362 529 839 1009 2.05e-28 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057681.t1 AT1G55320 69.173 266 60 4 547 799 147 403 2.61e-123 380
MS.gene057681.t1 AT1G55320 68.797 266 61 3 547 799 363 619 4.38e-120 379
MS.gene057681.t1 AT4G04885 44.068 531 246 15 39 546 303 805 9.54e-113 362
MS.gene057681.t1 AT5G23050 49.438 267 113 4 547 800 358 615 3.05e-79 270
MS.gene057681.t1 AT5G23050 47.881 236 101 4 547 769 358 584 9.41e-65 227
MS.gene057681.t1 AT1G66500 34.722 360 169 14 210 534 66 394 1.93e-45 169
MS.gene057681.t1 AT5G43620 36.111 288 134 10 279 534 119 388 1.77e-42 160
MS.gene057681.t1 AT2G36480 32.941 170 106 3 362 531 786 947 1.76e-23 107
MS.gene057681.t1 AT2G36480 32.941 170 106 3 362 531 786 947 1.76e-23 107

Find 224 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTGTATGCAGGTTATTT+TGG 0.133360 4.4:-35143598 MS.gene057681:intron
CTATTGCTGGCCTACAAATT+TGG 0.134649 4.4:-35144734 MS.gene057681:CDS
CCATGATGAGGTTTACTTTA+AGG 0.136545 4.4:-35142729 MS.gene057681:CDS
GGATGCAGGTGTTACCATTT+TGG 0.185825 4.4:-35143753 MS.gene057681:CDS
TACATAATGCACATGTATTT+TGG 0.199596 4.4:+35148389 None:intergenic
AAACAGCTCTTTCAGTTTCC+TGG 0.223690 4.4:-35149022 MS.gene057681:CDS
CCCCTGCGGCCTCAAGCTTT+TGG 0.226216 4.4:-35143463 MS.gene057681:CDS
TCTCCTCAAGGCCATGGTTT+TGG 0.228125 4.4:-35144640 MS.gene057681:CDS
TTATATCATTGCTTTCTAAA+TGG 0.249459 4.4:-35144688 MS.gene057681:CDS
TGCGAGAGGGCTTCTGCTTA+TGG 0.258664 4.4:+35148545 None:intergenic
ACGCGGAGCATGCACATTTA+TGG 0.263678 4.4:+35149419 None:intergenic
TTATTCCATTAGGCATATTA+AGG 0.280367 4.4:+35148302 None:intergenic
CATCAAGAGCACTTCGTATT+GGG 0.284804 4.4:+35144791 None:intergenic
CATGATGAGGTTTACTTTAA+GGG 0.286532 4.4:-35142728 MS.gene057681:CDS
GCACTTGCTGCAACGGTAAC+AGG 0.291227 4.4:+35149534 None:intergenic
TGACGAACCTTCAGAAGATT+TGG 0.298615 4.4:+35148700 None:intergenic
AAGAGCTGTTTGCAATTCTT+TGG 0.300209 4.4:+35149034 None:intergenic
GCTTACTGCCAGAAGTTTCT+TGG 0.305569 4.4:+35149727 None:intergenic
GCTTGGAACGGTTCCCAAAA+TGG 0.305618 4.4:+35143739 None:intergenic
ATGTGTATTAGCAGGTTCTT+AGG 0.307494 4.4:-35142304 MS.gene057681:intron
ATGCCTGTTGTTATTCCATT+AGG 0.324024 4.4:+35148292 None:intergenic
GGTTTATTCCCTGTCTCTCT+TGG 0.324602 4.4:-35142779 MS.gene057681:CDS
ATTTATTCCACCAATGTTCA+TGG 0.328257 4.4:+35142214 None:intergenic
TATTGCTGGCCTACAAATTT+GGG 0.330034 4.4:-35144733 MS.gene057681:CDS
AGAAACCATTACTTCTACTA+TGG 0.330076 4.4:+35149589 None:intergenic
CCTCCACATAATTCAATGAT+TGG 0.330215 4.4:+35143520 None:intergenic
ATAACTGATGAAGAATTCTT+TGG 0.332236 4.4:+35149858 None:intergenic
ATGCATTTAGCATGAATAAT+GGG 0.333257 4.4:+35148250 None:intergenic
GATTCGAATGGATTCCTAAC+AGG 0.333615 4.4:+35149360 None:intergenic
ACAAATTTGGGATGGGTGAT+AGG 0.340700 4.4:-35144721 MS.gene057681:CDS
CGAGTCCAAGGAATAGCTTT+TGG 0.340865 4.4:+35144820 None:intergenic
TCTAGGAAGATCGCCATATA+AGG 0.342211 4.4:+35148660 None:intergenic
TCTGCAATCAGTGCCTTATA+TGG 0.351640 4.4:-35148673 MS.gene057681:CDS
CTGCTATTGACGTTCAAGTT+GGG 0.355743 4.4:-35144762 MS.gene057681:CDS
GGTTTCTTGCAACCAACTAT+TGG 0.360158 4.4:-35149573 MS.gene057681:CDS
TTCCAAGCTTAGTGAAATCT+TGG 0.364156 4.4:-35143724 MS.gene057681:CDS
TATGCATTTAGCATGAATAA+TGG 0.367648 4.4:+35148249 None:intergenic
GGTAGAGCATGTGGGCCAGC+TGG 0.367975 4.4:+35148976 None:intergenic
AACAGCTCTTTCAGTTTCCT+GGG 0.369668 4.4:-35149021 MS.gene057681:CDS
TCAGCGTCAAGGGTGTTTCC+TGG 0.373151 4.4:-35149504 MS.gene057681:CDS
TGGCAGTAAGCCTTTGTTAG+TGG 0.376982 4.4:-35149715 MS.gene057681:CDS
TCAAAAGTTGCTGGCAGAAC+TGG 0.381258 4.4:+35149453 None:intergenic
TAGGCATCAATTAGAGCTCT+TGG 0.388106 4.4:+35149765 None:intergenic
TGTGACTTCTGTGCTCATCT+TGG 0.389046 4.4:+35148605 None:intergenic
GGCATAACAAGTTGTGCATT+TGG 0.389349 4.4:+35149072 None:intergenic
TTCCAAGATTTCACTAAGCT+TGG 0.392624 4.4:+35143722 None:intergenic
GGGACTTCCCGTAATGTAAC+TGG 0.392704 4.4:+35143483 None:intergenic
GCTGATTAGGCAACTGATAC+AGG 0.398235 4.4:+35149238 None:intergenic
AAGTTGGGGATGTCTATTGC+TGG 0.403132 4.4:-35144747 MS.gene057681:CDS
AAGGGAATGCCCTTGCATAA+AGG 0.411507 4.4:-35142710 MS.gene057681:CDS
TAACAAGTTGTGCATTTGGC+AGG 0.415660 4.4:+35149076 None:intergenic
GACAAAAGCCAAGAAACTTC+TGG 0.416293 4.4:-35149735 MS.gene057681:CDS
TTGCCACCTTTACCTCCAGC+TGG 0.419948 4.4:-35148991 MS.gene057681:CDS
GTGACTTCTGTGCTCATCTT+GGG 0.422924 4.4:+35148606 None:intergenic
GATTGGGATTAGGTAGAATC+TGG 0.424134 4.4:+35148953 None:intergenic
AATCCAATCATTGAATTATG+TGG 0.424565 4.4:-35143523 MS.gene057681:CDS
AAAGAAAGAAGATGAAGAGT+TGG 0.425274 4.4:-35148431 MS.gene057681:CDS
CCATCAAGAGCACTTCGTAT+TGG 0.425421 4.4:+35144790 None:intergenic
GGATTCTTCGATGGAAATAT+AGG 0.431337 4.4:+35149381 None:intergenic
CTGTTTGCAATTCTTTGGCC+TGG 0.431824 4.4:+35149039 None:intergenic
CCAATACGAAGTGCTCTTGA+TGG 0.432832 4.4:-35144790 MS.gene057681:CDS
GAAGGACCAGGATTGGGATT+AGG 0.436097 4.4:+35148943 None:intergenic
GTGGACAAACGCCACCATTC+CGG 0.438771 4.4:-35149182 MS.gene057681:CDS
GTATACCTTAATATGCCTAA+TGG 0.439382 4.4:-35148307 MS.gene057681:CDS
CTCCAAAAGCTTGAGGCCGC+AGG 0.443253 4.4:+35143461 None:intergenic
GTGGCTAGGTGCAGAGGCAT+TGG 0.444504 4.4:-35148500 MS.gene057681:CDS
TTCATCTTGGTGGGAATACT+CGG 0.444524 4.4:-35149890 MS.gene057681:CDS
GCTTGCGTCCAGTTACATTA+CGG 0.444712 4.4:-35143491 MS.gene057681:CDS
GACACAGAAGGACCAGGATT+GGG 0.445177 4.4:+35148937 None:intergenic
GCGTTTGTCCACTGTTATGC+GGG 0.451893 4.4:+35149193 None:intergenic
CTTGCGTCCAGTTACATTAC+GGG 0.455274 4.4:-35143490 MS.gene057681:CDS
CGCCTCGTTTCAAAAGTTGC+TGG 0.461501 4.4:+35149444 None:intergenic
TGGAAATATAGGAGTTATGC+TGG 0.461860 4.4:+35149392 None:intergenic
TAAGTAAATTGTGACGCTGC+TGG 0.462016 4.4:+35149141 None:intergenic
TTCCATCGAAGAATCCTGTT+AGG 0.463431 4.4:-35149374 MS.gene057681:CDS
GCTCATCTTGGGACTTGAAT+CGG 0.465641 4.4:+35148617 None:intergenic
CTTTGGCCTGGCGAAGGAAG+TGG 0.465990 4.4:+35149051 None:intergenic
TTGGGAGCTGAATCAGCTCC+AGG 0.466055 4.4:-35148481 MS.gene057681:CDS
TCAGATGCTCCAAGAGAGAC+AGG 0.473039 4.4:+35142770 None:intergenic
ATCACCCATCCCAAATTTGT+AGG 0.473339 4.4:+35144724 None:intergenic
TCACTGTAAAACTTGTCAAA+AGG 0.474303 4.4:+35148361 None:intergenic
GCCTTTGTTAGTGGAGCGAC+CGG 0.474907 4.4:-35149706 MS.gene057681:CDS
GATATAATCAAGAGAATGGT+TGG 0.477992 4.4:-35142272 MS.gene057681:CDS
ATGCCTAATGGAATAACAAC+AGG 0.480072 4.4:-35148295 MS.gene057681:CDS
GGCCCAAGGTGTTATGTCAT+TGG 0.480316 4.4:-35148866 MS.gene057681:CDS
CCATGTTCATGCACAGGAAA+AGG 0.480750 4.4:+35149291 None:intergenic
TCTGATGAGAAACCAATAGT+TGG 0.483041 4.4:+35149561 None:intergenic
ACTTATAGCACTTCTTTGCC+AGG 0.484285 4.4:-35149123 MS.gene057681:CDS
GCATCTCTTTCTTTGTACCA+TGG 0.485412 4.4:-35144664 MS.gene057681:CDS
AGCACAGCATCAATGACAAC+TGG 0.485665 4.4:-35143433 MS.gene057681:CDS
TGCTATTGACGTTCAAGTTG+GGG 0.485865 4.4:-35144761 MS.gene057681:CDS
TGTGGGCCAGCTGGAGGTAA+AGG 0.486896 4.4:+35148985 None:intergenic
GAGATCCAAAAGCTATTCCT+TGG 0.489267 4.4:-35144825 MS.gene057681:CDS
TTAGCATTTATTGATTCGAA+TGG 0.491940 4.4:+35149348 None:intergenic
ATAGATTTAGTTACCAGCGC+AGG 0.494952 4.4:+35148832 None:intergenic
TGGCTAGGTGCAGAGGCATT+GGG 0.497013 4.4:-35148499 MS.gene057681:CDS
TACGAAGTGCTCTTGATGGA+TGG 0.497932 4.4:-35144786 MS.gene057681:CDS
TGACACAGAAGGACCAGGAT+TGG 0.501011 4.4:+35148936 None:intergenic
ATCAGACCGGGTAGCTGATT+AGG 0.502180 4.4:+35149225 None:intergenic
CTGAAGAGGAGGAGTTCGAT+TGG 0.502985 4.4:-35149638 MS.gene057681:CDS
GGCATGGACAGGTCGCAGTT+AGG 0.505187 4.4:-35148274 MS.gene057681:CDS
CCACAAGTTGTGCACTGTCT+AGG 0.505428 4.4:+35148643 None:intergenic
TCATCAAAACATCTCTTTCG+CGG 0.506613 4.4:+35149825 None:intergenic
TAATTTGATTAGTTCACTCA+TGG 0.508846 4.4:-35148887 MS.gene057681:CDS
GTTCCTTGTGTTGGTGAAGT+GGG 0.510523 4.4:-35142800 MS.gene057681:CDS
ACTATGATTGTTCAGGATGC+AGG 0.510990 4.4:-35143767 MS.gene057681:intron
CTAGAAAATGTTCCTTGTGT+TGG 0.513511 4.4:-35142809 MS.gene057681:CDS
AGAGCTCTAATTGATGCCTA+TGG 0.514260 4.4:-35149762 MS.gene057681:CDS
AGCCAATGACATAACACCTT+GGG 0.518658 4.4:+35148864 None:intergenic
ACAGTGATGAAACGGAGGAT+TGG 0.518817 4.4:-35148345 MS.gene057681:CDS
ACGGAGGATTGGATGTATAG+AGG 0.522404 4.4:-35148334 MS.gene057681:CDS
TGGAGATATAATCAAGAGAA+TGG 0.526748 4.4:-35142276 MS.gene057681:CDS
CCATCACAAAATATTCAGAC+GGG 0.528028 4.4:+35148215 None:intergenic
AGCACAGAAGTCACATGGAT+TGG 0.529243 4.4:-35148597 MS.gene057681:CDS
TCCAAAAGCTTGAGGCCGCA+GGG 0.534212 4.4:+35143462 None:intergenic
ATTTCATATCAGGAGGTGGC+AGG 0.534414 4.4:-35145627 MS.gene057681:intron
TGAAGAGGAGGAGTTCGATT+GGG 0.536389 4.4:-35149637 MS.gene057681:CDS
GCTGCTATTGACGTTCAAGT+TGG 0.538506 4.4:-35144763 MS.gene057681:CDS
CTCATAGCAGCACAATGTCC+TGG 0.542263 4.4:+35149105 None:intergenic
GGCTAATCAAGCCCCTGCGC+TGG 0.542720 4.4:-35148845 MS.gene057681:intron
AACAAGTTGTGCATTTGGCA+GGG 0.544007 4.4:+35149077 None:intergenic
AATATCCAACAGTTGGCTGT+TGG 0.544187 4.4:+35148911 None:intergenic
AAATTAGAATATCCAACAGT+TGG 0.545058 4.4:+35148904 None:intergenic
TGCAACTACTTCAGCGTCAA+GGG 0.545212 4.4:-35149514 MS.gene057681:CDS
GAATAACAACAGGCATGGAC+AGG 0.551052 4.4:-35148285 MS.gene057681:CDS
GCAATTCTTTGGCCTGGCGA+AGG 0.555122 4.4:+35149045 None:intergenic
GTTATGCCACTTCCTTCGCC+AGG 0.555543 4.4:-35149057 MS.gene057681:CDS
TAGATTTAGTTACCAGCGCA+GGG 0.556762 4.4:+35148833 None:intergenic
ACAAGTTGTGCATTTGGCAG+GGG 0.557390 4.4:+35149078 None:intergenic
GGTGGCTATATCATCGTACA+AGG 0.557509 4.4:-35142251 MS.gene057681:CDS
AGATACCTTTCCTTTATGCA+AGG 0.557986 4.4:+35142700 None:intergenic
GGCGTTTGTCCACTGTTATG+CGG 0.558202 4.4:+35149192 None:intergenic
TAGCAGGTTCTTAGGAGACA+TGG 0.558384 4.4:-35142296 MS.gene057681:intron
GTGCAACTACTTCAGCGTCA+AGG 0.559076 4.4:-35149515 MS.gene057681:CDS
AGCAGAAGCCCTCTCGCAAG+TGG 0.560089 4.4:-35148540 MS.gene057681:CDS
AGAATCTGGGGTAGAGCATG+TGG 0.560420 4.4:+35148967 None:intergenic
AAAGTTGCTGGCAGAACTGG+TGG 0.562453 4.4:+35149456 None:intergenic
TTCCTAACAGGATTCTTCGA+TGG 0.563320 4.4:+35149372 None:intergenic
CAAGTTGTGCATTTGGCAGG+GGG 0.563383 4.4:+35149079 None:intergenic
AGTAGTTGCACTTGCTGCAA+CGG 0.563425 4.4:+35149527 None:intergenic
AATCAAAAGATGCTTGCTGA+TGG 0.564765 4.4:-35149915 None:intergenic
GATACCTTTCCTTTATGCAA+GGG 0.565038 4.4:+35142701 None:intergenic
ATTCTACCTAATCCCAATCC+TGG 0.565745 4.4:-35148949 MS.gene057681:CDS
TGGCTTGACACAGAAGGACC+AGG 0.566253 4.4:+35148931 None:intergenic
CTACTATGGTCTACTAGTGT+CGG 0.567210 4.4:+35149603 None:intergenic
GGCTGTTGGCTTGACACAGA+AGG 0.567803 4.4:+35148925 None:intergenic
TCCATCACAAAATATTCAGA+CGG 0.568109 4.4:+35148214 None:intergenic
GCTGGCCTACAAATTTGGGA+TGG 0.568482 4.4:-35144729 MS.gene057681:CDS
TCAAGCCAACAGCCAACTGT+TGG 0.568482 4.4:-35148916 MS.gene057681:CDS
GACGCTGCTGGATCCTGAGA+TGG 0.569203 4.4:+35149153 None:intergenic
CGGGTTAGATGAAATCAGAC+CGG 0.570016 4.4:+35149212 None:intergenic
ATCAAGAGCACTTCGTATTG+GGG 0.570807 4.4:+35144792 None:intergenic
ACGAAGTGCTCTTGATGGAT+GGG 0.573019 4.4:-35144785 MS.gene057681:CDS
GTAGACCATAGTAGAAGTAA+TGG 0.573238 4.4:-35149594 MS.gene057681:CDS
ATTAGTTCACTCATGGCCCA+AGG 0.574805 4.4:-35148880 MS.gene057681:CDS
AAGCAATGATATAACAGAGT+AGG 0.574823 4.4:+35144697 None:intergenic
CATGGATCTCCTCAAGGCCA+TGG 0.575075 4.4:-35144646 MS.gene057681:CDS
CCTTAAAGTAAACCTCATCA+TGG 0.575288 4.4:+35142729 None:intergenic
TGTTCCTTGTGTTGGTGAAG+TGG 0.576111 4.4:-35142801 MS.gene057681:CDS
GTTTGATCCAAATCTTCTGA+AGG 0.578645 4.4:-35148707 MS.gene057681:CDS
GGAGTTATGCTGGGAGGACG+CGG 0.578950 4.4:+35149402 None:intergenic
CAGCTCTTTCAGTTTCCTGG+GGG 0.579833 4.4:-35149019 MS.gene057681:CDS
TCCGGTCGCTCCACTAACAA+AGG 0.582967 4.4:+35149705 None:intergenic
GCAGGAAAATCCCCTTGCAC+AGG 0.583936 4.4:-35145609 MS.gene057681:intron
ACAGCTCTTTCAGTTTCCTG+GGG 0.586349 4.4:-35149020 MS.gene057681:CDS
CCTAGACAGTGCACAACTTG+TGG 0.586792 4.4:-35148643 MS.gene057681:CDS
TCAACACCATGTTCATGCAC+AGG 0.588454 4.4:+35149285 None:intergenic
CTGGCCTACAAATTTGGGAT+GGG 0.589172 4.4:-35144728 MS.gene057681:CDS
GTTGGCAGTTCTTGCTGACG+AGG 0.590256 4.4:-35148413 MS.gene057681:CDS
AAAGTAAACCTCATCATGGT+CGG 0.590749 4.4:+35142733 None:intergenic
TTCCCAAAACCATGGCCTTG+AGG 0.591486 4.4:+35144637 None:intergenic
TTGTACCATGGATCTCCTCA+AGG 0.591857 4.4:-35144652 MS.gene057681:CDS
CTGAATGCTCCAAAAGCTTG+AGG 0.595297 4.4:+35143454 None:intergenic
ATTGGGGCAAGTCGAGTCCA+AGG 0.596687 4.4:+35144808 None:intergenic
GACGATGTGGCTAGGTGCAG+AGG 0.597079 4.4:-35148506 MS.gene057681:CDS
CCAAAAGCTTGAGGCCGCAG+GGG 0.597788 4.4:+35143463 None:intergenic
GTGAGTGAGACGATGTGGCT+AGG 0.601495 4.4:-35148514 MS.gene057681:CDS
AGATTTCACTAAGCTTGGAA+CGG 0.603067 4.4:+35143727 None:intergenic
TTTGTCATTCTTGACGACAA+TGG 0.604567 4.4:-35143409 MS.gene057681:CDS
TAATGGAATAACAACAGGCA+TGG 0.604751 4.4:-35148290 MS.gene057681:CDS
AATAGTACCAGTGTGAGTAA+TGG 0.608110 4.4:-35149327 MS.gene057681:CDS
AGAGCATGTGGGCCAGCTGG+AGG 0.611285 4.4:+35148979 None:intergenic
GGAAATATAGGAGTTATGCT+GGG 0.611878 4.4:+35149393 None:intergenic
AATTAGACCATTACTCACAC+TGG 0.612003 4.4:+35149320 None:intergenic
CTCACAAACCACTTGCGAGA+GGG 0.612344 4.4:+35148532 None:intergenic
CTATATCATCGTACAAGGGA+GGG 0.614354 4.4:-35142246 MS.gene057681:CDS
GGGTTAGATGAAATCAGACC+GGG 0.617202 4.4:+35149213 None:intergenic
ATTGGGATTAGGTAGAATCT+GGG 0.617573 4.4:+35148954 None:intergenic
AATGCCCTTGCATAAAGGAA+AGG 0.620306 4.4:-35142705 MS.gene057681:intron
TAGCCAATGACATAACACCT+TGG 0.621234 4.4:+35148863 None:intergenic
CCAATCATTGAATTATGTGG+AGG 0.624312 4.4:-35143520 MS.gene057681:CDS
TGCATTTAGCATGAATAATG+GGG 0.625810 4.4:+35148251 None:intergenic
CATGGCCTTGAGGAGATCCA+TGG 0.627745 4.4:+35144647 None:intergenic
GAACAAATTTCCCAAAACCA+TGG 0.631785 4.4:+35144629 None:intergenic
GCTATATCATCGTACAAGGG+AGG 0.632238 4.4:-35142247 MS.gene057681:CDS
ATGGCAGAATACTGAAGAGG+AGG 0.632607 4.4:-35149649 MS.gene057681:CDS
ACTCACAAACCACTTGCGAG+AGG 0.632815 4.4:+35148531 None:intergenic
GATGACACCATGAACATTGG+TGG 0.633573 4.4:-35142221 MS.gene057681:CDS
AGCTCTTTCAGTTTCCTGGG+GGG 0.634048 4.4:-35149018 MS.gene057681:CDS
AGATGAGCACAGAAGTCACA+TGG 0.637659 4.4:-35148602 MS.gene057681:CDS
GGGCCAGCTGGAGGTAAAGG+TGG 0.639466 4.4:+35148988 None:intergenic
CAGTTGCCTAATCAGCTACC+CGG 0.639744 4.4:-35149231 MS.gene057681:CDS
ATAAGTTTATGACAACATCA+TGG 0.644139 4.4:-35149668 MS.gene057681:CDS
CATCTTGGGACTTGAATCGG+AGG 0.650361 4.4:+35148620 None:intergenic
GCTGATGACACCATGAACAT+TGG 0.650476 4.4:-35142224 MS.gene057681:CDS
TCAATATTAGAATTGAGACC+AGG 0.653967 4.4:+35149486 None:intergenic
GCTGAATGCCGACCATGATG+AGG 0.659320 4.4:-35142741 MS.gene057681:CDS
ATAATCAAGAGAATGGTTGG+TGG 0.659430 4.4:-35142269 MS.gene057681:CDS
ATCATGGCAGAATACTGAAG+AGG 0.665757 4.4:-35149652 MS.gene057681:CDS
AGATTTAGTTACCAGCGCAG+GGG 0.672730 4.4:+35148834 None:intergenic
TCATCTAACCCGCATAACAG+TGG 0.680332 4.4:-35149201 MS.gene057681:CDS
GAATCTGGGGTAGAGCATGT+GGG 0.680646 4.4:+35148968 None:intergenic
CATCACAAAATATTCAGACG+GGG 0.686925 4.4:+35148216 None:intergenic
TTGGGATTAGGTAGAATCTG+GGG 0.686989 4.4:+35148955 None:intergenic
AAACCCACTTCACCAACACA+AGG 0.689168 4.4:+35142797 None:intergenic
TTGCTGGCAGAACTGGTGGA+CGG 0.691595 4.4:+35149460 None:intergenic
GAGTTAAGCTATCTCAACAA+CGG 0.702080 4.4:-35149804 MS.gene057681:CDS
CAGATGCTCCAAGAGAGACA+GGG 0.703099 4.4:+35142771 None:intergenic
CAAATATTGATGATGATCTG+TGG 0.704903 4.4:-35143567 MS.gene057681:CDS
AATATAGGAGTTATGCTGGG+AGG 0.735215 4.4:+35149396 None:intergenic
GGTTTGTGAGTGAGACGATG+TGG 0.738502 4.4:-35148519 MS.gene057681:CDS
GTGGCTATATCATCGTACAA+GGG 0.741466 4.4:-35142250 MS.gene057681:CDS
ACATTACGGGAAGTCCCCTG+CGG 0.755485 4.4:-35143477 MS.gene057681:CDS
TTTGCCAACCGAGACCACTG+AGG 0.774441 4.4:-35148455 MS.gene057681:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.4 gene 35142208 35149928 35142208 ID=MS.gene057681
chr4.4 mRNA 35142208 35149928 35142208 ID=MS.gene057681.t1;Parent=MS.gene057681
chr4.4 exon 35148846 35149928 35148846 ID=MS.gene057681.t1.exon1;Parent=MS.gene057681.t1
chr4.4 CDS 35148846 35149928 35148846 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
chr4.4 exon 35148207 35148746 35148207 ID=MS.gene057681.t1.exon2;Parent=MS.gene057681.t1
chr4.4 CDS 35148207 35148746 35148207 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
chr4.4 exon 35145610 35145637 35145610 ID=MS.gene057681.t1.exon3;Parent=MS.gene057681.t1
chr4.4 CDS 35145610 35145637 35145610 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
chr4.4 exon 35144627 35144847 35144627 ID=MS.gene057681.t1.exon4;Parent=MS.gene057681.t1
chr4.4 CDS 35144627 35144847 35144627 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
chr4.4 exon 35143711 35143774 35143711 ID=MS.gene057681.t1.exon5;Parent=MS.gene057681.t1
chr4.4 CDS 35143711 35143774 35143711 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
chr4.4 exon 35143399 35143607 35143399 ID=MS.gene057681.t1.exon6;Parent=MS.gene057681.t1
chr4.4 CDS 35143399 35143607 35143399 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
chr4.4 exon 35142706 35142858 35142706 ID=MS.gene057681.t1.exon7;Parent=MS.gene057681.t1
chr4.4 CDS 35142706 35142858 35142706 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
chr4.4 exon 35142208 35142312 35142208 ID=MS.gene057681.t1.exon8;Parent=MS.gene057681.t1
chr4.4 CDS 35142208 35142312 35142208 ID=cds.MS.gene057681.t1;Parent=MS.gene057681.t1
Gene Sequence

>MS.gene057681

ATGCTTGCTGATGGTTTTCATCTTGGTGGGAATACTCGGAGCGAAATACCAAAGAATTCTTCATCAGTTATCTCAAACATTCCGCGAAAGAGATGTTTTGATGAGTTAAGCTATCTCAACAACGGACAACCGCGTCAAAATCCAAGAGCTCTAATTGATGCCTATGGTAGTGACAAAAGCCAAGAAACTTCTGGCAGTAAGCCTTTGTTAGTGGAGCGACCGGATAAAAACAGTATGGATAAGTTTATGACAACATCATGGCAGAATACTGAAGAGGAGGAGTTCGATTGGGAAGATATGAGTCCGACACTAGTAGACCATAGTAGAAGTAATGGTTTCTTGCAACCAACTATTGGTTTCTCATCAGAAAAGCCTGTTACCGTTGCAGCAAGTGCAACTACTTCAGCGTCAAGGGTGTTTCCTGGTCTCAATTCTAATATTGAGTACCGTCCACCAGTTCTGCCAGCAACTTTTGAAACGAGGCGCTCCATAAATGTGCATGCTCCGCGTCCTCCCAGCATAACTCCTATATTTCCATCGAAGAATCCTGTTAGGAATCCATTCGAATCAATAAATGCTAATAGTACCAGTGTGAGTAATGGTCTAATTAACAGACCTTTTCCTGTGCATGAACATGGTGTTGAGAACAACAATACCAGTAAAAGAAACCTGTATCAGTTGCCTAATCAGCTACCCGGTCTGATTTCATCTAACCCGCATAACAGTGGACAAACGCCACCATTCCGGTTTTTTCCATCTCAGGATCCAGCAGCGTCACAATTTACTTATAGCACTTCTTTGCCAGGACATTGTGCTGCTATGAGTAACCCCCTGCCAAATGCACAACTTGTTATGCCACTTCCTTCGCCAGGCCAAAGAATTGCAAACAGCTCTTTCAGTTTCCTGGGGGGAGCTTTGCCACCTTTACCTCCAGCTGGCCCACATGCTCTACCCCAGATTCTACCTAATCCCAATCCTGGTCCTTCTGTGTCAAGCCAACAGCCAACTGTTGGATATTCTAATTTGATTAGTTCACTCATGGCCCAAGGTGTTATGTCATTGGCTAATCAAGCCCCTGCGCTGGTAACTAAATCTATCCTTAGTTTAATTTTTATATCCTATTTTCCTTCCATTTTTGTTTGATTGCGTACCAATTTTGCAATTATATTATTATGTTTGTAGGATTTTGTTGGCATTGAGTTTGATCCAAATCTTCTGAAGGTTCGTCATGAATCTGCAATCAGTGCCTTATATGGCGATCTTCCTAGACAGTGCACAACTTGTGGCCTCCGATTCAAGTCCCAAGATGAGCACAGAAGTCACATGGATTGGCATGTGACTAAGAACCGTATGTCTAAAATCCATAAGCAGAAGCCCTCTCGCAAGTGGTTTGTGAGTGAGACGATGTGGCTAGGTGCAGAGGCATTGGGAGCTGAATCAGCTCCAGGTTTTTTGCCAACCGAGACCACTGAGGAAAAGAAAGAAGATGAAGAGTTGGCAGTTCTTGCTGACGAGGACCAAAATACATGTGCATTATGTAAAGAACCTTTTGACAAGTTTTACAGTGATGAAACGGAGGATTGGATGTATAGAGGAGCTGTATACCTTAATATGCCTAATGGAATAACAACAGGCATGGACAGGTCGCAGTTAGGCCCCATTATTCATGCTAAATGCATATCTGAATCTACCCCGTCTGAATATTTTGTGATGGATGAAGGGGTATGTGTTTCTTGTATTATTACTTTTCTATGCATTGTCTTGTTTTTGTTCCACTTTATGTCTTTAATTCATTGACTGACACATGTTTTTGGGGGAGAACAATACTAGTTGACTAGATTTCTTTGCAACTTTATGTTTTTTCTTTCCCATTGGTAAAACTTGTTTTTGAGGGAGATGTCCAGAATTCAGCAGCCATATTGTTCTTTTGGTGACTTCTCTTCAAGTGAGAATCAATCCATTATATCGTAAATCGCATGTTTTGGCTGAAATCCAAATGCAATGTCCTGTTTTTTCCGTCTTCTATGTCATGTCTTGCAAATGAGGATATCATGGTTTAAAATAGCTTGCAACACTAGGCATGGTAAAAACTGCAAATATATGTGCCTATTTAATGGCTGCCAACTTCACATGATGTTATTATTTCAAGTGGACTTTTGCATGTGCTGATGTTCTTCAAGCAGAGCAAATATTTATTCTTTTGTTTTCCTTGTATGCAGGGCACCTATGAAGAAGGCAGCCAAAGAAAACGAATGCAGAGCTGACTAAAGTAGTCCATTTATGTTCGCTGTAAGCCTGTAACTTACTAGTTTCGGATCAATGTATGCTCAATGTATGCAGGGCCTCTTGCCTAAATTATGTACATACATGTATAGATGCTCCTGTGTAACATGCATATCTTATTGCAGTCAAAAGTTTGCATTTAACCTGCAAATTTTCAGTACCTTACATTTATTGTCATTTCTTTTACTTCATTTTTGGCAGGAATTTGGCAAAGGATCCATGAGCACTTACTGTTTGAATTGTTATATCCAATTTAAATTGTGCATTGATGAATGAATGAGAATACTACAGATATTTGATGTTTTTTTTCCTTCTATAATTATTTCTGCTAGACATTTTATTTTAATGTCATTGGCTCTTTCTATAAGTTATTTTATTACTTTTTTCTCTATTATTTTGTGGTTCCTATGAATGAAAGTTTTTTTATAAGGTAAGAGATGGAGTCACCAACTATTTGTCTCTTTGTCCTTAAAAAAGAGATATCATTAATTCGTTTTACTATATTAGTATCTACAATCTAAATCAAAAGCTTAATTAACTATATAAAAAAAATTAGTATCTACAATATTGACTAAACAAGACTTATTAGTTCGAAATATAGGGCGCTATCTTTCACACGAGCCAAATTATCGCAAAGATCACTAAAGAATGTAAATTAGTTAATTAATCAAACAAGTGATTATACATAAACATGATAACTTATCCTATCAAACTCCTCTTATCCAGACAGTCAAACAAAGTTATAGGTTGTATTGTATTTGAATCCACACTTTTTGTTCATGAAAACATAGTGACAAACACCACAAGAAATTCACATTTCAAGGTTCCAAGTTCGGATCCAAATAAGATGTCTCCCATACCGATATTGATATTGCCAGTCAAATTAGGTCTCATGAACTGAATCCAAACCTTTTTTCTGTGTGTTGTCTCATTCCTTTGAACTCTAAAACGTTGCTTCTCCCTCACTATGCAGTGTTACTATGAAACACTGAATCTTCAAACACATTTCATCCACAATTGTAACGATTAACTCAAATTTCTTCGAAGTGTCATTTTTTATTATTTTATTTTCTCATGAGGTGCTATTGGGAAATATTTGGATTTCTACACACAATTGAATCAATTAACGCCAAACTTGGTTAAGGTGAAAGAATCGTGTTTGGTTCGCATAATGTTATAAATAAATTAGTAGTAACATTTTTTTTTTTTTTACAAATTATTAGTAGTAACAATGACTTGTAAGCAAAATTCCTAAAAAAAAAAAAAGATTTATAAGCAAAAGATAGCTCAATTATCTTAAAAAGAAATATTGTAAAAAAAAGTGCTCGAACATGATCACTTTTTAAAGAGAGAAAGACTTGACAAAAAGGTTACAAGATCTAAGGTTTGTGTTTGTGATCTTAAAGACACTCAAATATTGTGTTACTTAATACTTGTTTGAGATGAAACGATAAAACTAAACACACTCAAATATTGTGATCTACGAATTTGAAAAAAGTGCTGAAATGCTTGATATAAAGAGCCTTAGGCTTTGTTTGGGAGTTTGGAGGGGAGGGTAGGTGAGGGTTTTGGAAAAAAGGAAGGAGCAAGTGGAAGAAATAGACGACATTGAAGGAGAGGGCTTTGGAGGTTAATTTTTTACTCATAATAAAAAATCCTCCATCATTTGGGGGAACTCAAAAATTGTATTGGGGGAGGGTTTTGGGGGGGTTAGGGAGGGTTTATATGAATTTTTCAAATTTAATTATATTCTTAATATTTAAAGTAGAGAGAGAGGGAAAAGAAAATCAAGCAAGTATACTGCGAGAAAAGTGAAACTTGGGACGAAGCGAGTCCCTCCCTCGAAGAGTTAGCCTGATTACATTTTTGTTTCCTTCATTCAGTTTCTGCAATTTCTGGGTTAACTCCCAGTTTTCATCAATATTAGAGTGGGTTCTATCAATTTCCAATTTTTTAACAAATTAAAATTGTATTTCACTCAGCTTTTGTTTTCAGTATTTCATATCAGGAGGTGGCAGGAAAATCCCCTTGCACAGGTAAAATAAATCACTAGTACTTGTTGCTGCATTTTTGGCAGCACTTTAATTTTTGAATACCAAAATCATACTTTACTTGGGTCTGGGTCACTGTTATGATGCCATAGTCTGATTTATCTATTTAAAGATAGTTGGCATGTTAAGTGAAAATAAAGTTTATTTAAGATAGTTAGGTTATGTACTGGTCGTCTATTGATGCATTATTTAATTAATGTTTCAATTCTGGATGCTGATATAGAAAATACTTACTCGACTACTTCTCTCAGACTGCTTTACTGTCCTTAACTTGAGCTTGATAATACTGTTTGAATGGTTGTTAGATGGATTTACAACCGTTCTTTAAGCACATATATCATTTGAGATCTCATTCTGCATATTTGTCTTTGCAACTGAGGCAGCTGCAAGTAAAGTTATTGTGCTCACTGAGGCAGCTGCAATTAAGGGAAGAAGACTCGTCTCTCTTCTGGAAGTCAGAGCTCCAGTTATTGCATATTGCAATGAATTCATTAGACTATACCTTAATTTAATTAAGTGGTCCCTTGTACATACTAAATACTCACAGCAAAATATCCCGAAAATGGGGTCAAATGAGACAAATTGAGAAATTTCATGTTTTCTCTTTCGTAAGTTTTGTTTAATCTGACCTTAATATACTATTCTCATCTGGAACCACAGATAAAGATTATCCCTTATTATGTATGACATATCAGGTAGAGACCACTCATTATAATGATTTTCTGGTTATATTTTGGCTTTTATCAGGAGATCCAAAAGCTATTCCTTGGACTCGACTTGCCCCAATACGAAGTGCTCTTGATGGATGGGCTGCTATTGACGTTCAAGTTGGGGATGTCTATTGCTGGCCTACAAATTTGGGATGGGTGATAGGACCTACTCTGTTATATCATTGCTTTCTAAATGGTGCATCTCTTTCTTTGTACCATGGATCTCCTCAAGGCCATGGTTTTGGGAAATTTGTTCAGGTGAGTCTCCAATTTAATATTTCATTACTTTTATAATTTTGCCATACATAGATGATTAATTTATTTAGTATTTGCTTCTGCATATGATGGTTATCAATTATTGTTTTGATCAACTAAAATGTTGGAACAACAAACAAAAATATAAACGCTGATTTGTAATGAGAACCATTACTACAGTTCTCCTCACGATTACTGATTTGTAAAATAATGGAGGAAATAATGGGAATGAGAAACGGAGAGGATCCACCGCAGAGTATAAAGACCACCTCACCACGAAAATCTGTTTCATTCCTATAGTTGACCATTCTCGATGGAATAAGGTTGGCGTCCGACTCTATATCATCATCACCTCAAATCCACTATTCATTTACTATCATCAAACTTTGACCCAAAAAGCTGGTTAAAGAACTAATGTTTGGGGATGTTACATAGATGAAACACGTTTATGATAATCAGTCTCCCCTGCATATCTCTCAAATCCAATATTCCACAAGGGAACCAAACATTTGAGATAGAATGTCATTTCATTGAAGGAAGGATCTTACCTAAAGACATTATCACTAGCTTCATAAATTGGAAGATGTGTTCCCTTTAAATTAGTTATATATGTAACAAATATGATTTATAATCTTCAGTTGGAGGGGGAGTGTTCGAAATAGTTTAAATAGGGTAATGATTCTAAGTAGTAACAGTAACCGTACATCGTTGTGTCTATACAGCATTCTCAGATTTACTCATTCTCAGATTTATCTCAGATTCACAGATTCTATAACATGCCTATCATCTATTCTCCTTCTCAGCAAATACATCACACCAAGGCACAACTTTACTCATTGTACTATGATTGTTCAGGATGCAGGTGTTACCATTTTGGGAACCGTTCCAAGCTTAGTGAAATCTTGGAAGAGTACTCGATGTATGGTGGGCTTGGATTGGACAAAAATAAAGTAATATCTATTGAAAGTGTAAAGAATAAAATAGCTACTAAGAAAGATGAACTAAACTGCCCTTGTATGCAGGTTATTTTGGAGAAACGTCAAATATTGATGATGATCTGTGGCTTTCTTCAAAGTCATATTACAATCCAATCATTGAATTATGTGGAGGAACTGAGCTTGCGTCCAGTTACATTACGGGAAGTCCCCTGCGGCCTCAAGCTTTTGGAGCATTCAGCACAGCATCAATGACAACTGGCTTTGTCATTCTTGACGACAATGGAGTTCCTTATGTAAGAGCTTTTTCTGGATAAGTTATATTCTTTGGTCATGATTTTATTTACTTGTGCTGCTAGTCTGCCACTTCTCACCACATTACATTGTGCCAATACATCAGTATAGTTTTTTTGTGGCAAAGGTGATGGATGTGTGCTTACTTCATCTTCCAGACTAGGGAATGGAAAGGTTATTATGAACTATGAAGCACTGATAACACACAAACACTGGACATTGATAACAATTTTAAAAATTGAATTAATTGAATGTTATCACATGTGTACGCCTCTGATCAGAAGTGTCAGTGCTACAGAGTATGTTGGCGTGATAATGGCCAATAATACACCCTAGTCTCAAAATCTATGATCCATAGTTCCTAATGGCCATCAATTTGAATTCTGCACATGAATCTTTTTTCAATACTTGTTTCTGTACAGGTCTAGTTCTTATGCTAGCCCTATGTTCACCACCTTTTTCTTGTAACTTTCAGTCCCTATTGACTCTTAAATTTATTGATAGGTTTTCTGATTTATTGCTCTCTTTGACCAATATGACAGCTAGAAAATGTTCCTTTTTTGAAGAAACTAGAAAATGTTCCTTGTGTTGGTGAAGTGGGTTTATTCCCTGTCTCTCTTGGAGCATCTGATAGATTGCTGAATGCCGACCATGATGAGGTTTACTTTAAGGGAATGCCCTTGCATAAAGGAAAGGTATCTACCACTTCAATTAATATGATTAGAATGATTTCCCCTTTTTGTCTCGACATACCAACACATTTCGATACAACTACTTTGAAATTTCAGTTCATTCATTCTCGCTATGCGTCCTAAAGGCTTTTAAGAATTTTTTTTTCATGTGCAATTACCAAATTACACATCGAACATCGAAATTAGATCGAGCAAAAAGATAGGAAAATCTTTTATTCTGAACTTTTGGATAAATCAAATGTCCAAAAGAACCGTATATTAGTTAGATATACATTAAGGATTTTCAAACAATCCCTTCATTATAAATTACATAGGCTTAATTTGTAATTTTGACTAGTCTCATTCTGCTATTATTGCTATAACTTGTCATGTCTTTCATGATATGTGTATTAGCAGGTTCTTAGGAGACATGGAGATATAATCAAGAGAATGGTTGGTGGCTATATCATCGTACAAGGGAGGGCTGATGACACCATGAACATTGGTGGAATAAATGCGTAA
Protein sequence

>MS.gene057681.t1

MLADGFHLGGNTRSEIPKNSSSVISNIPRKRCFDELSYLNNGQPRQNPRALIDAYGSDKSQETSGSKPLLVERPDKNSMDKFMTTSWQNTEEEEFDWEDMSPTLVDHSRSNGFLQPTIGFSSEKPVTVAASATTSASRVFPGLNSNIEYRPPVLPATFETRRSINVHAPRPPSITPIFPSKNPVRNPFESINANSTSVSNGLINRPFPVHEHGVENNNTSKRNLYQLPNQLPGLISSNPHNSGQTPPFRFFPSQDPAASQFTYSTSLPGHCAAMSNPLPNAQLVMPLPSPGQRIANSSFSFLGGALPPLPPAGPHALPQILPNPNPGPSVSSQQPTVGYSNLISSLMAQGVMSLANQAPALDFVGIEFDPNLLKVRHESAISALYGDLPRQCTTCGLRFKSQDEHRSHMDWHVTKNRMSKIHKQKPSRKWFVSETMWLGAEALGAESAPGFLPTETTEEKKEDEELAVLADEDQNTCALCKEPFDKFYSDETEDWMYRGAVYLNMPNGITTGMDRSQLGPIIHAKCISESTPSEYFVMDEGEVAGKSPCTGDPKAIPWTRLAPIRSALDGWAAIDVQVGDVYCWPTNLGWVIGPTLLYHCFLNGASLSLYHGSPQGHGFGKFVQDAGVTILGTVPSLVKSWKSTRCYFGETSNIDDDLWLSSKSYYNPIIELCGGTELASSYITGSPLRPQAFGAFSTASMTTGFVILDDNGVPYLENVPFLKKLENVPCVGEVGLFPVSLGASDRLLNADHDEVYFKGMPLHKGKVLRRHGDIIKRMVGGYIIVQGRADDTMNIGGINA