Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057930.t1 | XP_024632961.1 | 95.8 | 310 | 13 | 0 | 1 | 310 | 1 | 310 | 8.60E-156 | 559.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057930.t1 | Q9SZL8 | 29.3 | 259 | 165 | 7 | 50 | 308 | 72 | 312 | 2.7e-25 | 117.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057930.t1 | A0A072TE13 | 87.7 | 310 | 23 | 1 | 1 | 310 | 1 | 295 | 6.4e-153 | 549.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene057930.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057930.t1 | MTR_0558s0020 | 89.355 | 310 | 18 | 1 | 1 | 310 | 1 | 295 | 0.0 | 578 |
| MS.gene057930.t1 | MTR_1g083640 | 93.357 | 286 | 19 | 0 | 1 | 286 | 1 | 286 | 0.0 | 564 |
| MS.gene057930.t1 | MTR_8g446020 | 77.419 | 310 | 15 | 2 | 1 | 310 | 1 | 255 | 1.58e-170 | 473 |
| MS.gene057930.t1 | MTR_2g014100 | 87.747 | 253 | 22 | 2 | 1 | 252 | 1 | 245 | 5.09e-163 | 454 |
| MS.gene057930.t1 | MTR_6g090505 | 90.435 | 230 | 22 | 0 | 81 | 310 | 1 | 230 | 4.12e-156 | 441 |
| MS.gene057930.t1 | MTR_0337s0040 | 73.188 | 276 | 20 | 2 | 1 | 276 | 1 | 222 | 2.35e-138 | 390 |
| MS.gene057930.t1 | MTR_3g008240 | 83.945 | 218 | 20 | 1 | 1 | 218 | 1 | 203 | 1.68e-132 | 375 |
| MS.gene057930.t1 | MTR_4g040430 | 84.404 | 218 | 14 | 2 | 1 | 218 | 1 | 198 | 1.45e-131 | 372 |
| MS.gene057930.t1 | MTR_5g071590 | 86.339 | 183 | 22 | 1 | 1 | 183 | 1 | 180 | 2.13e-116 | 333 |
| MS.gene057930.t1 | MTR_1g055240 | 94.366 | 142 | 8 | 0 | 107 | 248 | 3 | 144 | 4.00e-98 | 286 |
| MS.gene057930.t1 | MTR_0019s0290 | 88.889 | 153 | 14 | 1 | 100 | 249 | 8 | 160 | 5.79e-97 | 283 |
| MS.gene057930.t1 | MTR_1281s0010 | 95.413 | 109 | 5 | 0 | 35 | 143 | 1 | 109 | 1.40e-72 | 219 |
| MS.gene057930.t1 | MTR_8g046060 | 78.125 | 96 | 12 | 2 | 1 | 95 | 91 | 178 | 3.55e-39 | 137 |
| MS.gene057930.t1 | MTR_4g008820 | 76.842 | 95 | 7 | 1 | 1 | 95 | 125 | 204 | 9.39e-38 | 142 |
| MS.gene057930.t1 | MTR_6g051425 | 30.522 | 249 | 158 | 4 | 56 | 304 | 22 | 255 | 4.71e-32 | 122 |
| MS.gene057930.t1 | MTR_1g070355 | 24.756 | 307 | 210 | 5 | 3 | 307 | 24 | 311 | 4.56e-22 | 96.7 |
| MS.gene057930.t1 | MTR_0337s0050 | 80.556 | 36 | 7 | 0 | 275 | 310 | 14 | 49 | 8.18e-15 | 68.9 |
| MS.gene057930.t1 | MTR_0402s0040 | 25.946 | 185 | 118 | 4 | 65 | 249 | 40 | 205 | 2.54e-14 | 73.6 |
| MS.gene057930.t1 | MTR_1g013060 | 30.093 | 216 | 132 | 8 | 56 | 269 | 29 | 227 | 3.12e-14 | 71.2 |
| MS.gene057930.t1 | MTR_4g035820 | 22.692 | 260 | 176 | 5 | 56 | 310 | 80 | 319 | 5.45e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057930.t1 | AT4G38180 | 27.157 | 313 | 193 | 8 | 9 | 308 | 22 | 312 | 3.35e-26 | 109 |
| MS.gene057930.t1 | AT5G28530 | 25.000 | 260 | 165 | 6 | 56 | 303 | 60 | 301 | 1.40e-15 | 77.8 |
| MS.gene057930.t1 | AT5G28530 | 25.000 | 260 | 165 | 6 | 56 | 303 | 60 | 301 | 1.60e-15 | 77.4 |
| MS.gene057930.t1 | AT5G28530 | 25.000 | 260 | 165 | 6 | 56 | 303 | 60 | 301 | 1.60e-15 | 77.4 |
| MS.gene057930.t1 | AT1G10240 | 22.709 | 251 | 170 | 7 | 65 | 310 | 62 | 293 | 2.15e-12 | 68.2 |
| MS.gene057930.t1 | AT1G10240 | 22.709 | 251 | 170 | 7 | 65 | 310 | 62 | 293 | 2.15e-12 | 68.2 |
| MS.gene057930.t1 | AT4G15090 | 24.797 | 246 | 164 | 6 | 66 | 310 | 66 | 291 | 6.38e-11 | 63.5 |
| MS.gene057930.t1 | AT4G15090 | 24.797 | 246 | 164 | 6 | 66 | 310 | 66 | 291 | 6.38e-11 | 63.5 |
| MS.gene057930.t1 | AT4G15090 | 24.797 | 246 | 164 | 6 | 66 | 310 | 66 | 291 | 6.38e-11 | 63.5 |
| MS.gene057930.t1 | AT4G15090 | 24.797 | 246 | 164 | 6 | 66 | 310 | 66 | 291 | 6.38e-11 | 63.5 |
| MS.gene057930.t1 | AT4G15090 | 24.797 | 246 | 164 | 6 | 66 | 310 | 66 | 291 | 6.38e-11 | 63.5 |
Find 41 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGTTGTTGGCCTGTTGTT+AGG | 0.292589 | 3.3:+14957210 | None:intergenic |
| TTACTTCTCGAAATGATCTA+AGG | 0.311482 | 3.3:+14957019 | None:intergenic |
| CAACTAGATTATTCTCTATT+CGG | 0.323831 | 3.3:-14956703 | MS.gene057930:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.341758 | 3.3:+14956653 | None:intergenic |
| AACAATAAATATCGGCCATT+AGG | 0.388497 | 3.3:-14956646 | None:intergenic |
| ACCGAACAAACAATAAATAT+CGG | 0.389395 | 3.3:-14956654 | MS.gene057930:CDS |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 3.3:-14956944 | MS.gene057930:CDS |
| GTCTTATGAAGTTATGGGTC+AGG | 0.415385 | 3.3:-14956938 | MS.gene057930:CDS |
| GAAGTTATGGGTCAGGGAGT+TGG | 0.426260 | 3.3:-14956931 | MS.gene057930:CDS |
| CGTTATGTTTGTAACAAAGA+AGG | 0.427485 | 3.3:-14957258 | MS.gene057930:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 3.3:-14957006 | MS.gene057930:CDS |
| TCCATGATTCTTTATTGCTA+GGG | 0.444266 | 3.3:+14957421 | None:intergenic |
| TTCCATGATTCTTTATTGCT+AGG | 0.445442 | 3.3:+14957420 | None:intergenic |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 3.3:-14957141 | MS.gene057930:CDS |
| ATGTGTATAAGATATATAGA+TGG | 0.452819 | 3.3:-14957089 | MS.gene057930:CDS |
| ACATCAACTTCATCTGATAG+TGG | 0.459514 | 3.3:-14957546 | MS.gene057930:CDS |
| GCTCTTATAAATTCTAAAGC+TGG | 0.462891 | 3.3:-14956976 | MS.gene057930:CDS |
| ACCTGTGGACTGTCTGTTGT+TGG | 0.465317 | 3.3:+14957198 | None:intergenic |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 3.3:-14956943 | MS.gene057930:CDS |
| GTTTACTTAATCCTAACAAC+AGG | 0.470860 | 3.3:-14957221 | MS.gene057930:CDS |
| TTTAAGTCTGAAAACCGAAA+AGG | 0.483010 | 3.3:+14956892 | None:intergenic |
| CCCCTAGCAATAAAGAATCA+TGG | 0.497001 | 3.3:-14957422 | MS.gene057930:CDS |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 3.3:+14957147 | None:intergenic |
| AGCTGGAATATATTTGAAGC+TGG | 0.507410 | 3.3:-14957124 | MS.gene057930:CDS |
| ACAAAACAAGAAATCTGATA+AGG | 0.511435 | 3.3:-14957292 | MS.gene057930:CDS |
| CTAAGGTAACACGAATGCTC+TGG | 0.517941 | 3.3:+14957036 | None:intergenic |
| GTCAGCATGAATCATTGCTA+GGG | 0.522802 | 3.3:+14957461 | None:intergenic |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 3.3:-14957354 | MS.gene057930:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 3.3:-14957247 | MS.gene057930:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.528498 | 3.3:-14957082 | MS.gene057930:CDS |
| GAATGCTCTGGTTTATGTAA+TGG | 0.534744 | 3.3:+14957048 | None:intergenic |
| CCATGATTCTTTATTGCTAG+GGG | 0.537011 | 3.3:+14957422 | None:intergenic |
| TGTCAGCATGAATCATTGCT+AGG | 0.540740 | 3.3:+14957460 | None:intergenic |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 3.3:-14957246 | MS.gene057930:CDS |
| GTTATGGGTCAGGGAGTTGG+AGG | 0.547221 | 3.3:-14956928 | MS.gene057930:CDS |
| TGTGTATAAGATATATAGAT+GGG | 0.601002 | 3.3:-14957088 | MS.gene057930:CDS |
| TCTTATGAAGTTATGGGTCA+GGG | 0.609153 | 3.3:-14956937 | MS.gene057930:CDS |
| GCCAACAACAGACAGTCCAC+AGG | 0.619933 | 3.3:-14957199 | MS.gene057930:CDS |
| TTCACAAACTCAATAACCTG+TGG | 0.626760 | 3.3:+14957183 | None:intergenic |
| TCACTGATTCATGATGAGAG+TGG | 0.657353 | 3.3:-14957501 | MS.gene057930:CDS |
| TCAGCATGAATCATTGCTAG+GGG | 0.681156 | 3.3:+14957462 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTGTATAAGATATATAGA+TGG | - | chr3.3:14957113-14957132 | MS.gene057930:CDS | 20.0% |
| !! | TGAATATCATAGTAAAAAGA+TGG | + | chr3.3:14957427-14957446 | None:intergenic | 20.0% |
| !! | TGTGTATAAGATATATAGAT+GGG | - | chr3.3:14957114-14957133 | MS.gene057930:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | - | chr3.3:14956827-14956846 | MS.gene057930:CDS | 20.0% |
| ! | ACAAAACAAGAAATCTGATA+AGG | - | chr3.3:14956910-14956929 | MS.gene057930:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | chr3.3:14957548-14957567 | MS.gene057930:CDS | 25.0% |
| ! | CAACTAGATTATTCTCTATT+CGG | - | chr3.3:14957499-14957518 | MS.gene057930:CDS | 25.0% |
| !! | ATAACACTGGATTTTCTATT+AGG | - | chr3.3:14956861-14956880 | MS.gene057930:CDS | 25.0% |
| !!! | AAGACATAGCTAGTATTTTT+TGG | - | chr3.3:14957461-14957480 | MS.gene057930:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | - | chr3.3:14956956-14956975 | MS.gene057930:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr3.3:14957259-14957278 | MS.gene057930:CDS | 30.0% | |
| CGTTATGTTTGTAACAAAGA+AGG | - | chr3.3:14956944-14956963 | MS.gene057930:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | chr3.3:14957552-14957571 | None:intergenic | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | - | chr3.3:14956848-14956867 | MS.gene057930:CDS | 30.0% | |
| GTTTACTTAATCCTAACAAC+AGG | - | chr3.3:14956981-14957000 | MS.gene057930:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | chr3.3:14956955-14956974 | MS.gene057930:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | chr3.3:14957120-14957139 | MS.gene057930:CDS | 30.0% | |
| TCCATGATTCTTTATTGCTA+GGG | + | chr3.3:14956784-14956803 | None:intergenic | 30.0% | |
| TTACTTCTCGAAATGATCTA+AGG | + | chr3.3:14957186-14957205 | None:intergenic | 30.0% | |
| TTCCATGATTCTTTATTGCT+AGG | + | chr3.3:14956785-14956804 | None:intergenic | 30.0% | |
| TTTAAGTCTGAAAACCGAAA+AGG | + | chr3.3:14957313-14957332 | None:intergenic | 30.0% | |
| !! | GCTCTTATAAATTCTAAAGC+TGG | - | chr3.3:14957226-14957245 | MS.gene057930:CDS | 30.0% |
| AATTCGTCAAAAAGAGATGG+TGG | - | chr3.3:14957348-14957367 | MS.gene057930:CDS | 35.0% | |
| ACATCAACTTCATCTGATAG+TGG | - | chr3.3:14956656-14956675 | MS.gene057930:CDS | 35.0% | |
| AGTAAAAAGATGGTTTCGAG+AGG | + | chr3.3:14957417-14957436 | None:intergenic | 35.0% | |
| CCATGATTCTTTATTGCTAG+GGG | + | chr3.3:14956783-14956802 | None:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr3.3:14957196-14957215 | MS.gene057930:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | - | chr3.3:14957045-14957064 | MS.gene057930:CDS | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | - | chr3.3:14957039-14957058 | MS.gene057930:CDS | 35.0% | |
| GACAATTCGTCAAAAAGAGA+TGG | - | chr3.3:14957345-14957364 | MS.gene057930:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr3.3:14957258-14957277 | MS.gene057930:CDS | 35.0% | |
| GTAAAAAGATGGTTTCGAGA+GGG | + | chr3.3:14957416-14957435 | None:intergenic | 35.0% | |
| TCTTATGAAGTTATGGGTCA+GGG | - | chr3.3:14957265-14957284 | MS.gene057930:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | - | chr3.3:14957044-14957063 | MS.gene057930:CDS | 35.0% | |
| TTCACAAACTCAATAACCTG+TGG | + | chr3.3:14957022-14957041 | None:intergenic | 35.0% | |
| ! | AGCTGGAATATATTTGAAGC+TGG | - | chr3.3:14957078-14957097 | MS.gene057930:CDS | 35.0% |
| ! | GAATGCTCTGGTTTATGTAA+TGG | + | chr3.3:14957157-14957176 | None:intergenic | 35.0% |
| !!! | GCTAGTATTTTTTGGTCAGA+TGG | - | chr3.3:14957469-14957488 | MS.gene057930:CDS | 35.0% |
| CCCCTAGCAATAAAGAATCA+TGG | - | chr3.3:14956780-14956799 | MS.gene057930:CDS | 40.0% | |
| GTCAGCATGAATCATTGCTA+GGG | + | chr3.3:14956744-14956763 | None:intergenic | 40.0% | |
| GTCTTATGAAGTTATGGGTC+AGG | - | chr3.3:14957264-14957283 | MS.gene057930:CDS | 40.0% | |
| TCAGCATGAATCATTGCTAG+GGG | + | chr3.3:14956743-14956762 | None:intergenic | 40.0% | |
| TGTCAGCATGAATCATTGCT+AGG | + | chr3.3:14956745-14956764 | None:intergenic | 40.0% | |
| ! | GCACAGAGAATCTTCCTTTT+CGG | - | chr3.3:14957296-14957315 | MS.gene057930:CDS | 40.0% |
| !! | TCACTGATTCATGATGAGAG+TGG | - | chr3.3:14956701-14956720 | MS.gene057930:CDS | 40.0% |
| CTAAGGTAACACGAATGCTC+TGG | + | chr3.3:14957169-14957188 | None:intergenic | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | - | chr3.3:14957049-14957068 | MS.gene057930:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | + | chr3.3:14957058-14957077 | None:intergenic | 45.0% | |
| ! | CGTCAAAAAGAGATGGTGGT+TGG | - | chr3.3:14957352-14957371 | MS.gene057930:CDS | 45.0% |
| ! | TCTGTTGTTGGCCTGTTGTT+AGG | + | chr3.3:14956995-14957014 | None:intergenic | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr3.3:14957061-14957080 | MS.gene057930:CDS | 45.0% |
| GAAGTTATGGGTCAGGGAGT+TGG | - | chr3.3:14957271-14957290 | MS.gene057930:CDS | 50.0% | |
| ! | ACCTGTGGACTGTCTGTTGT+TGG | + | chr3.3:14957007-14957026 | None:intergenic | 50.0% |
| GCCAACAACAGACAGTCCAC+AGG | - | chr3.3:14957003-14957022 | MS.gene057930:CDS | 55.0% | |
| GTTATGGGTCAGGGAGTTGG+AGG | - | chr3.3:14957274-14957293 | MS.gene057930:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 14956647 | 14957577 | 14956647 | ID=MS.gene057930 |
| chr3.3 | mRNA | 14956647 | 14957577 | 14956647 | ID=MS.gene057930.t1;Parent=MS.gene057930 |
| chr3.3 | exon | 14956647 | 14957577 | 14956647 | ID=MS.gene057930.t1.exon1;Parent=MS.gene057930.t1 |
| chr3.3 | CDS | 14956647 | 14957577 | 14956647 | ID=cds.MS.gene057930.t1;Parent=MS.gene057930.t1 |
| Gene Sequence |
| Protein sequence |
