AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058100.t1 MTR_5g007600 92.188 256 20 0 2 257 41 296 9.86e-168 467
MS.gene058100.t1 MTR_5g007600 85.577 104 15 0 82 185 223 326 5.71e-59 191
MS.gene058100.t1 MTR_1g046680 79.310 174 36 0 12 185 66 239 2.46e-89 265
MS.gene058100.t1 MTR_1g046680 72.000 75 21 0 183 257 135 209 8.41e-34 123
MS.gene058100.t1 MTR_8g013950 41.398 186 98 4 7 185 112 293 5.82e-33 123
MS.gene058100.t1 MTR_8g013950 57.143 49 21 0 209 257 215 263 1.27e-12 67.4
MS.gene058100.t1 MTR_2g094600 44.720 161 57 4 44 181 128 279 1.03e-31 119
MS.gene058100.t1 MTR_2g094600 65.116 43 15 0 208 250 204 246 7.74e-13 67.8
MS.gene058100.t1 MTR_1g094930 54.545 99 45 0 83 181 112 210 5.03e-31 115
MS.gene058100.t1 MTR_1g094930 49.296 71 34 2 181 250 108 177 4.24e-12 64.7
MS.gene058100.t1 MTR_8g076340 70.000 70 21 0 116 185 216 285 3.20e-30 115
MS.gene058100.t1 MTR_8g076340 62.500 40 15 0 218 257 216 255 8.10e-12 64.3
MS.gene058100.t1 MTR_1g094930 54.545 99 45 0 83 181 207 305 3.24e-30 115
MS.gene058100.t1 MTR_1g094930 49.296 71 34 2 181 250 203 272 8.95e-12 64.7
MS.gene058100.t1 MTR_3g109760 73.913 69 18 0 117 185 206 274 3.42e-30 115
MS.gene058100.t1 MTR_3g109760 47.458 59 31 0 199 257 186 244 4.82e-12 65.5
MS.gene058100.t1 MTR_6g032830 73.846 65 17 0 117 181 191 255 3.80e-30 114
MS.gene058100.t1 MTR_6g032830 64.103 39 14 0 219 257 191 229 4.31e-11 62.0
MS.gene058100.t1 MTR_1g006650 71.831 71 20 0 118 188 234 304 5.71e-30 115
MS.gene058100.t1 MTR_1g006650 77.419 31 7 0 220 250 234 264 3.66e-11 62.8
MS.gene058100.t1 MTR_7g085410 75.000 64 16 0 118 181 188 251 1.49e-29 112
MS.gene058100.t1 MTR_7g085410 65.789 38 13 0 220 257 188 225 8.02e-11 61.2
MS.gene058100.t1 MTR_4g058910 62.651 83 30 1 100 181 295 377 1.37e-28 112
MS.gene058100.t1 MTR_4g058910 54.000 50 23 0 208 257 302 351 8.12e-11 62.0
MS.gene058100.t1 MTR_1g116010 68.182 66 21 0 116 181 162 227 2.04e-28 108
MS.gene058100.t1 MTR_1g116010 72.727 33 9 0 218 250 162 194 2.34e-11 62.8
MS.gene058100.t1 MTR_4g071590 70.588 68 20 0 118 185 211 278 3.93e-28 109
MS.gene058100.t1 MTR_4g071590 39.286 112 52 4 152 257 147 248 2.24e-11 63.2
MS.gene058100.t1 MTR_4g471590 70.588 68 20 0 118 185 211 278 3.93e-28 109
MS.gene058100.t1 MTR_4g471590 39.286 112 52 4 152 257 147 248 2.24e-11 63.2
MS.gene058100.t1 MTR_5g021340 66.667 72 24 0 118 189 300 371 4.65e-28 111
MS.gene058100.t1 MTR_3g117380 59.259 81 33 0 109 189 220 300 7.75e-28 109
MS.gene058100.t1 MTR_7g446110 54.839 62 28 0 118 179 187 248 1.38e-17 80.1
MS.gene058100.t1 MTR_7g445980 53.846 65 29 1 116 179 31 95 8.88e-17 74.3
MS.gene058100.t1 MTR_7g445950 51.613 62 30 0 118 179 120 181 1.51e-15 73.2
MS.gene058100.t1 MTR_7g446070 50.000 62 31 0 118 179 132 193 7.38e-15 71.6
MS.gene058100.t1 MTR_5g020230 34.234 111 55 3 53 150 49 154 2.40e-11 62.4
MS.gene058100.t1 MTR_7g112330 57.143 42 18 0 112 153 16 57 7.79e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058100.t1 AT3G24050 42.574 202 85 5 8 181 59 257 1.50e-39 139
MS.gene058100.t1 AT3G24050 55.000 60 20 1 205 257 172 231 3.04e-14 71.2
MS.gene058100.t1 AT4G34680 47.857 140 58 4 57 185 112 247 1.13e-33 123
MS.gene058100.t1 AT4G34680 47.887 71 35 1 183 251 141 211 3.15e-12 65.5
MS.gene058100.t1 AT4G34680 47.857 140 58 4 57 185 112 247 1.13e-33 123
MS.gene058100.t1 AT4G34680 47.887 71 35 1 183 251 141 211 3.15e-12 65.5
MS.gene058100.t1 AT3G60530 44.681 141 69 3 52 185 87 225 8.64e-32 117
MS.gene058100.t1 AT3G60530 46.429 56 30 0 202 257 140 195 9.73e-11 60.8
MS.gene058100.t1 AT1G08010 51.667 120 50 3 72 183 166 285 1.12e-30 116
MS.gene058100.t1 AT1G08010 50.980 51 25 0 207 257 207 257 7.02e-11 62.0
MS.gene058100.t1 AT1G08010 51.667 120 50 3 72 183 166 285 1.12e-30 116
MS.gene058100.t1 AT1G08010 50.980 51 25 0 207 257 207 257 7.02e-11 62.0
MS.gene058100.t1 AT1G08010 51.667 120 50 3 72 183 166 285 1.12e-30 116
MS.gene058100.t1 AT1G08010 50.980 51 25 0 207 257 207 257 7.02e-11 62.0
MS.gene058100.t1 AT4G32890 72.464 69 19 0 117 185 196 264 1.46e-30 116
MS.gene058100.t1 AT4G32890 75.000 32 8 0 219 250 196 227 4.42e-11 62.4
MS.gene058100.t1 AT2G45050 40.571 175 78 5 34 185 75 246 8.86e-30 113
MS.gene058100.t1 AT5G25830 73.529 68 18 0 118 185 219 286 8.99e-30 114
MS.gene058100.t1 AT1G08000 40.909 176 73 6 33 181 110 281 1.97e-29 113
MS.gene058100.t1 AT1G08000 40.909 176 73 6 33 181 110 281 1.97e-29 113
MS.gene058100.t1 AT5G66320 72.059 68 19 0 118 185 249 316 3.61e-29 113
MS.gene058100.t1 AT5G66320 68.421 38 12 0 220 257 249 286 4.09e-11 62.8
MS.gene058100.t1 AT5G66320 72.059 68 19 0 118 185 249 316 3.61e-29 113
MS.gene058100.t1 AT5G66320 68.421 38 12 0 220 257 249 286 4.09e-11 62.8
MS.gene058100.t1 AT3G51080 72.059 68 19 0 118 185 221 288 5.70e-29 112
MS.gene058100.t1 AT3G45170 44.366 142 57 4 47 185 60 182 1.97e-28 108
MS.gene058100.t1 AT4G36240 39.153 189 69 7 7 181 71 227 6.04e-28 107
MS.gene058100.t1 AT3G54810 70.312 64 19 0 118 181 229 292 1.80e-26 105
MS.gene058100.t1 AT3G54810 70.312 64 19 0 118 181 229 292 1.80e-26 105
MS.gene058100.t1 AT2G28340 64.516 62 22 0 120 181 193 254 5.88e-23 95.5
MS.gene058100.t1 AT3G06740 63.889 36 13 0 118 153 41 76 5.38e-11 60.1

Find 70 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTGCAGGGCGGTATTC+AGG 0.237442 5.4:-2928795 None:intergenic
ATGTGCTTAGATTGGGGATT+AGG 0.251282 5.4:+2929430 MS.gene058100:CDS
TGTGCTTAGATTGGGGATTA+GGG 0.343205 5.4:+2929431 MS.gene058100:CDS
GGTTTGCTTCGATTGTTCTT+TGG 0.359998 5.4:-2928952 None:intergenic
GTTGTTTCCACGCGAATTGA+TGG 0.373020 5.4:-2928608 None:intergenic
CACATCACATGTGCTTAGAT+TGG 0.390703 5.4:+2929422 MS.gene058100:CDS
ATGCATGTGGGGTTAGGTAT+AGG 0.393134 5.4:+2928757 MS.gene058100:CDS
CGAAATATCTGGTTGAATAC+AGG 0.393474 5.4:-2928415 None:intergenic
CCACGCGAATTGATGGTTCC+AGG 0.401734 5.4:-2928601 None:intergenic
AGCTGGAAATGCATCTTTGT+TGG 0.402382 5.4:-2928370 None:intergenic
GCTGTTTCCACGTGAATTGA+CGG 0.414591 5.4:-2928914 None:intergenic
TTTGTAATGCATGTGGGGTT+AGG 0.415099 5.4:+2928751 MS.gene058100:CDS
TCTTTGGATGCTTTGTTACT+CGG 0.415607 5.4:-2928936 None:intergenic
ACATCACATGTGCTTAGATT+GGG 0.417527 5.4:+2929423 MS.gene058100:CDS
TCATCAAATGTTTCAACAGC+TGG 0.422269 5.4:-2928387 None:intergenic
GCCTTGGCATTTCTGATACC+TGG 0.422871 5.4:+2928583 MS.gene058100:CDS
GAGCTGATAATACTCCTCTA+TGG 0.452228 5.4:+2928700 MS.gene058100:CDS
CTATGGCGTGCAGGTCCTGC+TGG 0.452250 5.4:+2928717 MS.gene058100:CDS
CTTGTGCTGTTCTCAACAAT+TGG 0.453321 5.4:-2928456 None:intergenic
GACTTGCTCCGTGCACGGAC+AGG 0.469909 5.4:-2928546 None:intergenic
TTTGTAATGCATGTGGGGTC+AGG 0.477301 5.4:+2929057 MS.gene058100:CDS
AACAGCATTACACTCTTAAG+TGG 0.478526 5.4:+2928504 MS.gene058100:CDS
GCCTTGGCATTTCTGACACA+TGG 0.480082 5.4:+2928889 MS.gene058100:CDS
ACATTTCCTCCCAATGGTTG+AGG 0.482486 5.4:-2928973 None:intergenic
CGAAGCAAACCTCAACCATT+GGG 0.485894 5.4:+2928964 MS.gene058100:CDS
GCGAAACAAACCTCAACCAT+TGG 0.490923 5.4:+2928657 MS.gene058100:CDS
ACATTGCCTCCCAATGGTTG+AGG 0.497491 5.4:-2928667 None:intergenic
TCGAAGCAAACCTCAACCAT+TGG 0.499266 5.4:+2928963 MS.gene058100:CDS
GATCCTCTACTGCAAACTCC+TGG 0.500842 5.4:-2928335 None:intergenic
CGAAACAAACCTCAACCATT+GGG 0.506442 5.4:+2928658 MS.gene058100:CDS
GTTCCAGGAGTTTGCAGTAG+AGG 0.515595 5.4:+2928332 MS.gene058100:CDS
AATACTCCGCAATGGCGTGC+AGG 0.516576 5.4:+2929014 MS.gene058100:CDS
TGAAGTTCCCTGTCCGTGCA+CGG 0.541394 5.4:+2928538 MS.gene058100:CDS
TTGCAGTAGAGGATCTTGAA+TGG 0.544677 5.4:+2928343 MS.gene058100:CDS
AATACTCCTCTATGGCGTGC+AGG 0.546436 5.4:+2928708 MS.gene058100:CDS
TGTGGGGTTAGGTATAGGTC+TGG 0.555845 5.4:+2928762 MS.gene058100:CDS
TATTTCCACTACTTTCCTGT+GGG 0.558023 5.4:-2928850 None:intergenic
TGCGAGACTTGCTCCGTGCA+CGG 0.562316 5.4:-2928551 None:intergenic
ACTTGCTCCGTGCACGGACA+GGG 0.574860 5.4:-2928545 None:intergenic
GCAGGACCTGCACGCCATAG+AGG 0.577006 5.4:-2928714 None:intergenic
AAAACACTTTGTAATGCATG+TGG 0.577073 5.4:+2929050 MS.gene058100:CDS
ACTTAAGAGTGTAATGCTGT+TGG 0.577340 5.4:-2928502 None:intergenic
TCTCGCAGTAAGCCTCGCCT+TGG 0.577592 5.4:+2928567 MS.gene058100:CDS
ATGATGACATTTCCTCCCAA+TGG 0.580868 5.4:-2928979 None:intergenic
AAAACACTTTGTAATGCATG+TGG 0.583893 5.4:+2928744 MS.gene058100:CDS
CATCACATGTGCTTAGATTG+GGG 0.585297 5.4:+2929424 MS.gene058100:CDS
CAATGGCGTGCAGGTCCCGA+TGG 0.590689 5.4:+2929023 MS.gene058100:CDS
ATTCGTAGTAAGCCTCGCCT+TGG 0.591249 5.4:+2928873 MS.gene058100:CDS
TTATTTCCACTACTTTCCTG+TGG 0.598127 5.4:-2928851 None:intergenic
AACACTTTGTAATGCATGTG+GGG 0.606830 5.4:+2928746 MS.gene058100:CDS
TAATTCCCACAGGAAAGTAG+TGG 0.608211 5.4:+2928845 MS.gene058100:CDS
TCCAGGTATCAGAAATGCCA+AGG 0.610134 5.4:-2928584 None:intergenic
AAACACTTTGTAATGCATGT+GGG 0.612005 5.4:+2928745 MS.gene058100:CDS
TCCATGTGTCAGAAATGCCA+AGG 0.617962 5.4:-2928890 None:intergenic
AACACTTTGTAATGCATGTG+GGG 0.618425 5.4:+2929052 MS.gene058100:CDS
AAACACTTTGTAATGCATGT+GGG 0.620936 5.4:+2929051 MS.gene058100:CDS
GGGAGGAAATGTCATCATTG+TGG 0.622937 5.4:+2928984 MS.gene058100:CDS
GGGAGGCAATGTCATCATTG+TGG 0.625233 5.4:+2928678 MS.gene058100:CDS
ATGTGCATTCTAATTCCCAC+AGG 0.631252 5.4:+2928835 MS.gene058100:CDS
GGCGGTATTCAGGTACAAGT+CGG 0.632650 5.4:-2928785 None:intergenic
AACAAACCTCAACCATTGGG+AGG 0.638620 5.4:+2928661 MS.gene058100:CDS
CCTGGAACCATCAATTCGCG+TGG 0.640359 5.4:+2928601 MS.gene058100:CDS
CATGGAACCGTCAATTCACG+TGG 0.644051 5.4:+2928907 MS.gene058100:CDS
TCGGGACCTGCACGCCATTG+CGG 0.648022 5.4:-2929020 None:intergenic
ATGATGACATTGCCTCCCAA+TGG 0.653586 5.4:-2928673 None:intergenic
GTGTCAGAAATGCCAAGGCG+AGG 0.655134 5.4:-2928885 None:intergenic
AGCAAACCTCAACCATTGGG+AGG 0.655985 5.4:+2928967 MS.gene058100:CDS
GTATCAGAAATGCCAAGGCG+AGG 0.673094 5.4:-2928579 None:intergenic
GAGCTGATAATACTCCGCAA+TGG 0.677467 5.4:+2929006 MS.gene058100:CDS
AAGTTGGGCTCTTTGCAGGG+CGG 0.700990 5.4:-2928803 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTTCTTTTTTACTT+TGG + chr5.4:2929271-2929290 MS.gene058100:intron 15.0%
!! AATCATTTCAATGTCAAATT+TGG + chr5.4:2929379-2929398 MS.gene058100:intron 20.0%
!! TTTGACATTGAAATGATTTA+AGG - chr5.4:2929377-2929396 None:intergenic 20.0%
!!! AAATTTGGTTTTTGCATTTT+AGG + chr5.4:2929394-2929413 MS.gene058100:intron 20.0%
!!! AAGTTAGCTTGCTATAAATT+AGG + chr5.4:2929246-2929265 MS.gene058100:intron 25.0%
AAAACACTTTGTAATGCATG+TGG + chr5.4:2928744-2928763 MS.gene058100:CDS 30.0%
AAACACTTTGTAATGCATGT+GGG + chr5.4:2928745-2928764 MS.gene058100:CDS 30.0%
!! CATGCATTACAAAGTGTTTT+CGG - chr5.4:2928744-2928763 None:intergenic 30.0%
!! CATGCATTACAAAGTGTTTT+TGG - chr5.4:2928744-2928763 None:intergenic 30.0%
!! TTTTGATTCTGCGAAATATC+TGG - chr5.4:2928429-2928448 None:intergenic 30.0%
!!! CAGTGAAAGTTTTGAAGTTA+GGG + chr5.4:2929348-2929367 MS.gene058100:intron 30.0%
AAAAAGTTGGGCTATTTGCA+GGG - chr5.4:2929115-2929134 None:intergenic 35.0%
AACACTTTGTAATGCATGTG+GGG + chr5.4:2928746-2928765 MS.gene058100:CDS 35.0%
AACAGCATTACACTCTTAAG+TGG + chr5.4:2928504-2928523 MS.gene058100:CDS 35.0%
AAGCAGAAGAGAATGAGAAT+GGG + chr5.4:2929182-2929201 MS.gene058100:intron 35.0%
AAGTGGTAGCTAGTGAAATA+TGG + chr5.4:2929215-2929234 MS.gene058100:intron 35.0%
ACATCACATGTGCTTAGATT+GGG + chr5.4:2929423-2929442 MS.gene058100:CDS 35.0%
AGAATGAGAATGGGATGAAA+TGG + chr5.4:2929191-2929210 MS.gene058100:intron 35.0%
ATAGCAAGCTAACTTCTACA+AGG - chr5.4:2929241-2929260 None:intergenic 35.0%
CGAAATATCTGGTTGAATAC+AGG - chr5.4:2928418-2928437 None:intergenic 35.0%
TATTTCCACTACTTTCCTGT+GGG - chr5.4:2928853-2928872 None:intergenic 35.0%
TCATCAAATGTTTCAACAGC+TGG - chr5.4:2928390-2928409 None:intergenic 35.0%
TTATTTCCACTACTTTCCTG+TGG - chr5.4:2928854-2928873 None:intergenic 35.0%
! ACAGGAAAGTAGTGGAAATA+AGG + chr5.4:2928853-2928872 MS.gene058100:CDS 35.0%
! ACTTAAGAGTGTAATGCTGT+TGG - chr5.4:2928505-2928524 None:intergenic 35.0%
! TCTTTGGATGCTTTGTTACT+CGG - chr5.4:2928939-2928958 None:intergenic 35.0%
!!! ACAAAGTGTTTTTGGACCAT+CGG - chr5.4:2929042-2929061 None:intergenic 35.0%
!!! GCAGTGAAAGTTTTGAAGTT+AGG + chr5.4:2929347-2929366 MS.gene058100:intron 35.0%
!!! TGAAAGTTTTGAAGTTAGGG+AGG + chr5.4:2929351-2929370 MS.gene058100:intron 35.0%
!!! TTTAGTCCTTTTTTCGCTGT+CGG + chr5.4:2929311-2929330 MS.gene058100:intron 35.0%
AAAAAGTTGGGCTCTTTGCA+GGG - chr5.4:2928809-2928828 None:intergenic 40.0%
AAACATCCGACAGCGAAAAA+AGG - chr5.4:2929320-2929339 None:intergenic 40.0%
AGCTGGAAATGCATCTTTGT+TGG - chr5.4:2928373-2928392 None:intergenic 40.0%
ATGATGACATTTCCTCCCAA+TGG - chr5.4:2928982-2929001 None:intergenic 40.0%
ATGTGCATTCTAATTCCCAC+AGG + chr5.4:2928835-2928854 MS.gene058100:CDS 40.0%
ATGTGCTTAGATTGGGGATT+AGG + chr5.4:2929430-2929449 MS.gene058100:CDS 40.0%
CAAAAAGTTGGGCTATTTGC+AGG - chr5.4:2929116-2929135 None:intergenic 40.0%
CACATCACATGTGCTTAGAT+TGG + chr5.4:2929422-2929441 MS.gene058100:CDS 40.0%
CATCACATGTGCTTAGATTG+GGG + chr5.4:2929424-2929443 MS.gene058100:CDS 40.0%
CGAAACAAACCTCAACCATT+GGG + chr5.4:2928658-2928677 MS.gene058100:CDS 40.0%
GAAGCAGAAGAGAATGAGAA+TGG + chr5.4:2929181-2929200 MS.gene058100:intron 40.0%
GAATGGGATGAAATGGAAAG+TGG + chr5.4:2929198-2929217 MS.gene058100:intron 40.0%
GAGCTGATAATACTCCTCTA+TGG + chr5.4:2928700-2928719 MS.gene058100:CDS 40.0%
GCACATTGCTGCAAAAAGTT+GGG - chr5.4:2928821-2928840 None:intergenic 40.0%
TAATTCCCACAGGAAAGTAG+TGG + chr5.4:2928845-2928864 MS.gene058100:CDS 40.0%
TGCACATTGCTGCAAAAAGT+TGG - chr5.4:2928822-2928841 None:intergenic 40.0%
TGTGCTTAGATTGGGGATTA+GGG + chr5.4:2929431-2929450 MS.gene058100:CDS 40.0%
TTGCAGTAGAGGATCTTGAA+TGG + chr5.4:2928343-2928362 MS.gene058100:CDS 40.0%
TTTGTAATGCATGTGGGGTT+AGG + chr5.4:2928751-2928770 MS.gene058100:CDS 40.0%
! CTTGTGCTGTTCTCAACAAT+TGG - chr5.4:2928459-2928478 None:intergenic 40.0%
! GGTTTGCTTCGATTGTTCTT+TGG - chr5.4:2928955-2928974 None:intergenic 40.0%
!!! CAAAGTGTTTTTGGACCATC+GGG - chr5.4:2929041-2929060 None:intergenic 40.0%
AACAAACCTCAACCATTGGG+AGG + chr5.4:2928661-2928680 MS.gene058100:CDS 45.0%
ACATTTCCTCCCAATGGTTG+AGG - chr5.4:2928976-2928995 None:intergenic 45.0%
ATGATGACATTGCCTCCCAA+TGG - chr5.4:2928676-2928695 None:intergenic 45.0%
ATGCATGTGGGGTTAGGTAT+AGG + chr5.4:2928757-2928776 MS.gene058100:CDS 45.0%
CAAAAAGTTGGGCTCTTTGC+AGG - chr5.4:2928810-2928829 None:intergenic 45.0%
CGAAGCAAACCTCAACCATT+GGG + chr5.4:2928964-2928983 MS.gene058100:CDS 45.0%
GAGCTGATAATACTCCGCAA+TGG + chr5.4:2929006-2929025 MS.gene058100:CDS 45.0%
GCGAAACAAACCTCAACCAT+TGG + chr5.4:2928657-2928676 MS.gene058100:CDS 45.0%
GGGAGGAAATGTCATCATTG+TGG + chr5.4:2928984-2929003 MS.gene058100:CDS 45.0%
TAATGCCCACAGGAAAGTAG+TGG + chr5.4:2929151-2929170 MS.gene058100:intron 45.0%
TCCAGGTATCAGAAATGCCA+AGG - chr5.4:2928587-2928606 None:intergenic 45.0%
TCCATGTGTCAGAAATGCCA+AGG - chr5.4:2928893-2928912 None:intergenic 45.0%
TCGAAGCAAACCTCAACCAT+TGG + chr5.4:2928963-2928982 MS.gene058100:CDS 45.0%
TTTGTAATGCATGTGGGGTC+AGG + chr5.4:2929057-2929076 MS.gene058100:CDS 45.0%
! GCTGTTTCCACGTGAATTGA+CGG - chr5.4:2928917-2928936 None:intergenic 45.0%
! GTTGTTTCCACGCGAATTGA+TGG - chr5.4:2928611-2928630 None:intergenic 45.0%
!! CGCTGTCGGATGTTTTTGAT+GGG + chr5.4:2929325-2929344 MS.gene058100:intron 45.0%
!! TCGCTGTCGGATGTTTTTGA+TGG + chr5.4:2929324-2929343 MS.gene058100:intron 45.0%
AAGTTGGGCTATTTGCAGGG+CGG - chr5.4:2929112-2929131 None:intergenic 50.0%
AATACTCCTCTATGGCGTGC+AGG + chr5.4:2928708-2928727 MS.gene058100:CDS 50.0%
ACATTGCCTCCCAATGGTTG+AGG - chr5.4:2928670-2928689 None:intergenic 50.0%
ACGTGCATTCTAATGCCCAC+AGG + chr5.4:2929141-2929160 MS.gene058100:intron 50.0%
AGCAAACCTCAACCATTGGG+AGG + chr5.4:2928967-2928986 MS.gene058100:CDS 50.0%
ATGCATGTGGGGTCAGGTAT+AGG + chr5.4:2929063-2929082 MS.gene058100:intron 50.0%
ATTCGTAGTAAGCCTCGCCT+TGG + chr5.4:2928873-2928892 MS.gene058100:CDS 50.0%
CATGGAACCGTCAATTCACG+TGG + chr5.4:2928907-2928926 MS.gene058100:CDS 50.0%
CTATTTGCAGGGCGGTATTC+AGG - chr5.4:2929104-2929123 None:intergenic 50.0%
GATCCTCTACTGCAAACTCC+TGG - chr5.4:2928338-2928357 None:intergenic 50.0%
GCACGTCGCTGCAAAAAGTT+GGG - chr5.4:2929127-2929146 None:intergenic 50.0%
GCCTTGGCATTTCTGACACA+TGG + chr5.4:2928889-2928908 MS.gene058100:CDS 50.0%
GCCTTGGCATTTCTGATACC+TGG + chr5.4:2928583-2928602 MS.gene058100:CDS 50.0%
GGCGGTATTCAGGTACAAGT+CGG - chr5.4:2928788-2928807 None:intergenic 50.0%
GGGAGGCAATGTCATCATTG+TGG + chr5.4:2928678-2928697 MS.gene058100:CDS 50.0%
GTATCAGAAATGCCAAGGCG+AGG - chr5.4:2928582-2928601 None:intergenic 50.0%
GTTCCAGGAGTTTGCAGTAG+AGG + chr5.4:2928332-2928351 MS.gene058100:CDS 50.0%
TGCACGTCGCTGCAAAAAGT+TGG - chr5.4:2929128-2929147 None:intergenic 50.0%
TGTGGGGTTAGGTATAGGTC+TGG + chr5.4:2928762-2928781 MS.gene058100:CDS 50.0%
!! CAAAGTGTTTTCGGACCAGC+AGG - chr5.4:2928735-2928754 None:intergenic 50.0%
AAGTTGGGCTCTTTGCAGGG+CGG - chr5.4:2928806-2928825 None:intergenic 55.0%
AATACTCCGCAATGGCGTGC+AGG + chr5.4:2929014-2929033 MS.gene058100:CDS 55.0%
CCTGGAACCATCAATTCGCG+TGG + chr5.4:2928601-2928620 MS.gene058100:CDS 55.0%
CTCTTTGCAGGGCGGTATTC+AGG - chr5.4:2928798-2928817 None:intergenic 55.0%
GTGTCAGAAATGCCAAGGCG+AGG - chr5.4:2928888-2928907 None:intergenic 55.0%
TGAAGTTCCCTGTCCGTGCA+CGG + chr5.4:2928538-2928557 MS.gene058100:CDS 55.0%
TGTGGGGTCAGGTATAGGTC+TGG + chr5.4:2929068-2929087 MS.gene058100:intron 55.0%
! CCACGCGAATTGATGGTTCC+AGG - chr5.4:2928604-2928623 None:intergenic 55.0%
ACTTGCTCCGTGCACGGACA+GGG - chr5.4:2928548-2928567 None:intergenic 60.0%
TCTCGCAGTAAGCCTCGCCT+TGG + chr5.4:2928567-2928586 MS.gene058100:CDS 60.0%
! TGCGAGACTTGCTCCGTGCA+CGG - chr5.4:2928554-2928573 None:intergenic 60.0%
CAATGGCGTGCAGGTCCCGA+TGG + chr5.4:2929023-2929042 MS.gene058100:CDS 65.0%
CTATGGCGTGCAGGTCCTGC+TGG + chr5.4:2928717-2928736 MS.gene058100:CDS 65.0%
GCAGGACCTGCACGCCATAG+AGG - chr5.4:2928717-2928736 None:intergenic 65.0%
TCGGGACCTGCACGCCATTG+CGG - chr5.4:2929023-2929042 None:intergenic 65.0%
! GACTTGCTCCGTGCACGGAC+AGG - chr5.4:2928549-2928568 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 2928330 2929473 2928330 ID=MS.gene058100
chr5.4 mRNA 2928330 2929473 2928330 ID=MS.gene058100.t1;Parent=MS.gene058100
chr5.4 exon 2928330 2929078 2928330 ID=MS.gene058100.t1.exon1;Parent=MS.gene058100.t1
chr5.4 CDS 2928330 2929078 2928330 ID=cds.MS.gene058100.t1;Parent=MS.gene058100.t1
chr5.4 exon 2929416 2929473 2929416 ID=MS.gene058100.t1.exon2;Parent=MS.gene058100.t1
chr5.4 CDS 2929416 2929473 2929416 ID=cds.MS.gene058100.t1;Parent=MS.gene058100.t1
Gene Sequence

>MS.gene058100

ATGTTCCAGGAGTTTGCAGTAGAGGATCTTGAATGGCTATCCAACAAAGATGCATTTCCAGCTGTTGAAACATTTGATGACTTTTCCTGTATTCAACCAGATATTTCGCAGAATCAAAAGATAGCACCAATTGTTGAGAACAGCACAAGTAGTAGCAACAGCAATAACAGTTCCAACAGCATTACACTCTTAAGTGGCTACAATCACGTGAAGTTCCCTGTCCGTGCACGGAGCAAGTCTCGCAGTAAGCCTCGCCTTGGCATTTCTGATACCTGGAACCATCAATTCGCGTGGAAACAACTGAGTAACAAAACTTCTAAAGAACAAGCGAAACAAACCTCAACCATTGGGAGGCAATGTCATCATTGTGGAGCTGATAATACTCCTCTATGGCGTGCAGGTCCTGCTGGTCCGAAAACACTTTGTAATGCATGTGGGGTTAGGTATAGGTCTGGCCGACTTGTACCTGAATACCGCCCTGCAAAGAGCCCAACTTTTTGCAGCAATGTGCATTCTAATTCCCACAGGAAAGTAGTGGAAATAATTCGTAGTAAGCCTCGCCTTGGCATTTCTGACACATGGAACCGTCAATTCACGTGGAAACAGCCGAGTAACAAAGCATCCAAAGAACAATCGAAGCAAACCTCAACCATTGGGAGGAAATGTCATCATTGTGGAGCTGATAATACTCCGCAATGGCGTGCAGGTCCCGATGGTCCAAAAACACTTTGTAATGCATGTGGGGTCAGGTATAGGTCTGGCCGACTTGTACCTGAATACCGCCCTGCAAATAGCCCAACTTTTTGCAGCGACGTGCATTCTAATGCCCACAGGAAAGTAGTGGAAATAAGGAAGCAGAAGAGAATGAGAATGGGATGAAATGGAAAGTGGTAGCTAGTGAAATATGGCCTTGTAGAAGTTAGCTTGCTATAAATTAGGATTTTGATTTTCTTTTTTACTTTGGTCTATTCTAATTGAAATTTTAGTCCTTTTTTCGCTGTCGGATGTTTTTGATGGGCAGTGAAAGTTTTGAAGTTAGGGAGGCCTTAAATCATTTCAATGTCAAATTTGGTTTTTGCATTTTAGGTTGCTCACATCACATGTGCTTAGATTGGGGATTAGGGAGAAGATGAGTTGCAAGTAG
Protein sequence

>MS.gene058100.t1

MFQEFAVEDLEWLSNKDAFPAVETFDDFSCIQPDISQNQKIAPIVENSTSSSNSNNSSNSITLLSGYNHVKFPVRARSKSRSKPRLGISDTWNHQFAWKQLSNKTSKEQAKQTSTIGRQCHHCGADNTPLWRAGPAGPKTLCNACGVRYRSGRLVPEYRPAKSPTFCSNVHSNSHRKVVEIIRSKPRLGISDTWNRQFTWKQPSNKASKEQSKQTSTIGRKCHHCGADNTPQWRAGPDGPKTLCNACGVRLLTSHVLRLGIREKMSCK