AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058204

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058204.t1 MTR_0402s0040 94.000 200 12 0 33 232 1 200 2.02e-131 398
MS.gene058204.t1 MTR_0402s0040 65.468 278 26 3 417 694 200 407 7.57e-102 321
MS.gene058204.t1 MTR_6g017200 90.260 154 15 0 1 154 1 154 2.30e-98 300
MS.gene058204.t1 MTR_2g070590 90.678 118 11 0 44 161 3 120 1.19e-70 226
MS.gene058204.t1 MTR_1g057920 78.448 116 25 0 32 147 8 123 3.52e-58 193
MS.gene058204.t1 MTR_7g113010 64.474 152 39 2 32 182 431 568 5.42e-52 190
MS.gene058204.t1 MTR_5g015480 62.338 154 53 2 34 186 36 185 8.60e-52 181
MS.gene058204.t1 MTR_0558s0020 34.008 247 143 5 72 298 50 296 2.51e-39 147
MS.gene058204.t1 MTR_6g051425 31.313 297 173 8 66 338 25 314 2.13e-38 146
MS.gene058204.t1 MTR_1g086270 56.522 115 38 1 352 466 1 103 1.14e-37 136
MS.gene058204.t1 MTR_0291s0060 77.381 84 19 0 25 108 2 85 3.77e-37 134
MS.gene058204.t1 MTR_8g446020 37.222 180 111 2 121 298 77 256 3.87e-34 131
MS.gene058204.t1 MTR_0137s0040 42.581 155 36 1 34 188 12 113 1.97e-32 121
MS.gene058204.t1 MTR_6g090505 35.071 211 121 4 103 297 20 230 1.00e-31 127
MS.gene058204.t1 MTR_1g070355 30.328 244 155 7 58 292 72 309 1.44e-26 115
MS.gene058204.t1 MTR_1g083640 29.386 228 141 5 62 269 55 282 6.16e-26 108
MS.gene058204.t1 MTR_7g074790 36.257 171 88 3 58 227 10 160 1.76e-23 100
MS.gene058204.t1 MTR_7g446520 31.837 245 148 5 61 303 91 318 5.25e-23 101
MS.gene058204.t1 MTR_4g035820 30.268 261 169 8 51 302 68 324 1.41e-22 103
MS.gene058204.t1 MTR_5g039760 36.410 195 95 7 62 253 14 182 1.78e-22 95.9
MS.gene058204.t1 MTR_1g050985 78.947 57 11 1 131 186 42 98 8.26e-22 91.3
MS.gene058204.t1 MTR_0337s0040 33.103 145 95 2 121 263 78 222 1.97e-19 88.2
MS.gene058204.t1 MTR_7g446480 36.364 176 108 4 134 306 140 314 1.60e-18 87.8
MS.gene058204.t1 MTR_1g008290 33.094 139 88 2 114 251 7 141 4.02e-18 82.0
MS.gene058204.t1 MTR_5g076070 25.275 273 181 8 29 286 18 282 1.34e-17 84.3
MS.gene058204.t1 MTR_7g446510 35.795 176 109 4 134 306 155 329 1.52e-17 85.1
MS.gene058204.t1 MTR_4g102720 30.128 156 74 2 58 213 7 127 2.82e-16 79.0
MS.gene058204.t1 MTR_4g067290 42.056 107 56 3 58 162 56 158 3.37e-16 79.0
MS.gene058204.t1 MTR_4g067290 42.056 107 56 3 58 162 56 158 3.78e-16 79.0
MS.gene058204.t1 MTR_4g067290 42.056 107 56 3 58 162 76 178 5.10e-16 79.0
MS.gene058204.t1 MTR_4g067290 42.056 107 56 3 58 162 74 176 5.12e-16 79.0
MS.gene058204.t1 MTR_4g067290 42.056 107 56 3 58 162 76 178 5.53e-16 79.0
MS.gene058204.t1 MTR_4g067290 42.056 107 56 3 58 162 116 218 7.53e-16 79.3
MS.gene058204.t1 MTR_7g061980 39.823 113 63 4 58 168 10 119 1.23e-15 74.3
MS.gene058204.t1 MTR_2g098920 40.187 107 58 3 58 162 80 182 1.48e-15 77.8
MS.gene058204.t1 MTR_1g091013 51.282 78 27 1 49 126 3 69 2.43e-15 71.6
MS.gene058204.t1 MTR_5g045210 33.858 127 78 1 589 715 20 140 5.09e-15 74.7
MS.gene058204.t1 MTR_6g022070 28.161 174 120 1 352 525 1 169 8.23e-15 76.6
MS.gene058204.t1 MTR_3g006370 38.462 104 62 2 58 160 50 152 3.34e-13 70.1
MS.gene058204.t1 MTR_2g014100 23.980 196 130 4 64 240 50 245 9.03e-13 69.3
MS.gene058204.t1 MTR_3g450850 24.723 271 179 10 47 297 32 297 2.94e-12 69.3
MS.gene058204.t1 MTR_1g019850 28.313 166 102 4 30 190 43 196 1.96e-11 65.1
MS.gene058204.t1 MTR_2g098880 36.190 105 64 2 58 160 61 164 2.12e-11 65.1
MS.gene058204.t1 MTR_0337s0060 26.437 174 97 4 448 617 21 167 2.13e-11 65.1
MS.gene058204.t1 MTR_2g098880 36.190 105 64 2 58 160 73 176 2.30e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058204.t1 AT4G38180 33.587 658 422 10 33 678 46 700 6.27e-120 377
MS.gene058204.t1 AT4G15090 29.213 623 428 6 58 674 51 666 1.24e-94 312
MS.gene058204.t1 AT4G15090 29.213 623 428 6 58 674 51 666 1.24e-94 312
MS.gene058204.t1 AT4G15090 29.213 623 428 6 58 674 51 666 1.24e-94 312
MS.gene058204.t1 AT4G15090 29.213 623 428 6 58 674 51 666 1.24e-94 312
MS.gene058204.t1 AT4G15090 29.213 623 428 6 58 674 51 666 1.24e-94 312
MS.gene058204.t1 AT1G76320 30.795 604 390 13 62 657 1 584 9.08e-90 295
MS.gene058204.t1 AT1G76320 30.795 604 390 13 62 657 1 584 1.23e-89 296
MS.gene058204.t1 AT1G76320 30.795 604 390 13 62 657 1 584 1.49e-89 296
MS.gene058204.t1 AT1G76320 30.795 604 390 13 62 657 1 584 1.49e-89 296
MS.gene058204.t1 AT3G22170 29.516 620 377 13 62 657 74 657 1.10e-86 291
MS.gene058204.t1 AT3G22170 29.516 620 377 13 62 657 74 657 1.10e-86 291
MS.gene058204.t1 AT2G27110 30.635 630 393 16 50 670 41 635 3.52e-84 284
MS.gene058204.t1 AT2G27110 30.635 630 393 16 50 670 41 635 3.52e-84 284
MS.gene058204.t1 AT3G06250 28.617 629 390 14 49 669 181 758 5.06e-79 268
MS.gene058204.t1 AT3G06250 28.617 629 390 14 49 669 181 758 5.06e-79 268
MS.gene058204.t1 AT3G06250 28.617 629 390 14 49 669 181 758 5.06e-79 268
MS.gene058204.t1 AT5G18960 28.594 640 399 17 38 669 193 782 3.48e-74 256
MS.gene058204.t1 AT5G18960 28.594 640 399 17 38 669 193 782 3.48e-74 256
MS.gene058204.t1 AT1G10240 28.682 645 424 15 52 674 42 672 1.61e-73 251
MS.gene058204.t1 AT1G10240 28.682 645 424 15 52 674 42 672 1.61e-73 251
MS.gene058204.t1 AT2G27110 33.634 443 279 9 236 670 55 490 4.65e-73 251
MS.gene058204.t1 AT2G32250 26.384 614 396 13 62 669 42 605 6.58e-69 241
MS.gene058204.t1 AT2G32250 26.710 614 394 13 62 669 42 605 7.99e-69 241
MS.gene058204.t1 AT2G32250 26.710 614 394 13 62 669 42 605 7.99e-69 241
MS.gene058204.t1 AT2G32250 26.710 614 394 13 62 669 1 564 8.39e-69 240
MS.gene058204.t1 AT2G32250 26.710 614 394 13 62 669 1 564 8.75e-69 240
MS.gene058204.t1 AT2G32250 26.384 614 396 13 62 669 42 605 9.65e-69 241
MS.gene058204.t1 AT2G32250 26.384 614 396 13 62 669 42 605 9.65e-69 241
MS.gene058204.t1 AT2G32250 26.384 614 396 13 62 669 42 605 9.65e-69 241
MS.gene058204.t1 AT4G19990 26.929 661 403 13 32 667 5 610 3.99e-61 219
MS.gene058204.t1 AT4G19990 27.619 630 386 12 63 667 26 610 4.12e-61 219
MS.gene058204.t1 AT4G19990 27.619 630 386 12 63 667 26 610 4.12e-61 219
MS.gene058204.t1 AT4G19990 26.929 661 403 13 32 667 5 610 4.88e-61 219
MS.gene058204.t1 AT4G19990 26.929 661 403 13 32 667 5 610 4.88e-61 219
MS.gene058204.t1 AT4G19990 26.706 674 409 14 19 667 11 624 5.40e-61 219
MS.gene058204.t1 AT4G38170 30.343 379 253 5 241 613 27 400 2.05e-60 213
MS.gene058204.t1 AT5G28530 26.435 575 385 14 58 607 55 616 1.13e-57 208
MS.gene058204.t1 AT1G80010 25.914 602 402 12 42 612 73 661 2.93e-56 205
MS.gene058204.t1 AT5G28530 26.261 575 382 14 58 607 55 612 3.24e-56 204
MS.gene058204.t1 AT5G28530 26.261 575 382 14 58 607 55 612 3.24e-56 204
MS.gene058204.t1 AT4G19990 27.349 596 363 12 97 667 3 553 2.17e-53 196
MS.gene058204.t1 AT1G52520 26.034 580 378 11 56 610 83 636 2.50e-53 196
MS.gene058204.t1 AT5G28530 25.267 562 345 17 58 607 55 553 9.02e-41 159
MS.gene058204.t1 AT5G28530 25.177 564 339 18 58 607 55 549 1.79e-39 155
MS.gene058204.t1 AT3G59470 36.424 151 84 3 25 165 27 175 2.89e-16 79.7
MS.gene058204.t1 AT3G59470 36.424 151 84 3 25 165 27 175 2.89e-16 79.7
MS.gene058204.t1 AT3G59470 36.424 151 84 3 25 165 39 187 3.51e-16 79.7
MS.gene058204.t1 AT4G12850 36.364 132 78 4 51 182 4 129 2.69e-15 73.9
MS.gene058204.t1 AT4G12850 38.739 111 64 3 51 161 4 110 9.03e-15 72.4
MS.gene058204.t1 AT4G12850 38.739 111 64 3 51 161 4 110 2.22e-14 72.4
MS.gene058204.t1 AT3G07500 40.000 105 62 1 58 162 33 136 7.66e-13 68.9
MS.gene058204.t1 AT2G43280 38.318 107 58 3 58 160 22 124 2.34e-12 67.0

Find 139 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATAACAAATATATTT+TGG 0.083882 2.2:+54387335 MS.gene058204:CDS
CGCTTAAGTATATAGGTTTC+AGG 0.211352 2.2:-54388354 None:intergenic
TTTGAAGTCTGATTTAAATT+TGG 0.211881 2.2:+54387954 MS.gene058204:CDS
TTCAGATTGTGTCAATAAAT+TGG 0.215486 2.2:+54386649 MS.gene058204:intron
CACCACATGCAAAACAAATA+AGG 0.219696 2.2:+54387414 MS.gene058204:CDS
ATAAATGATTATGGGTATTT+TGG 0.231818 2.2:+54387373 MS.gene058204:CDS
CACAATCCATGAAATGTTTC+TGG 0.237682 2.2:-54387640 None:intergenic
GCACAAATGATAAATGATTA+TGG 0.241903 2.2:+54387364 MS.gene058204:CDS
CTTTAGTGACTGTTTCCTTT+TGG 0.269240 2.2:-54387224 None:intergenic
TTTGGTATTCGTCGAGAATA+TGG 0.287007 2.2:+54386806 MS.gene058204:CDS
ATATATTCATGCAAATCTTT+TGG 0.296908 2.2:-54387133 None:intergenic
CTTGACTTGGTTATGACTCT+TGG 0.305137 2.2:-54388983 None:intergenic
AAGTATGTGGATGGAGAATC+AGG 0.314708 2.2:+54388591 MS.gene058204:CDS
TCTGCTCTAGGCTCCCCTTT+TGG 0.322934 2.2:-54386917 None:intergenic
TGTCCATCGCTTAAGTATAT+AGG 0.330247 2.2:-54388361 None:intergenic
CATTTCATGGATTGTGCACT+TGG 0.334162 2.2:+54387647 MS.gene058204:CDS
AATGAGTATAGTAAAAGAAT+TGG 0.336174 2.2:+54386782 MS.gene058204:CDS
ATGGTCGGAGACGGCGGCGG+CGG 0.340105 2.2:-54386153 None:intergenic
TGGAACTCCTCAAATATTCT+AGG 0.341058 2.2:-54388120 None:intergenic
CATTGTACCGCTTCATGAAA+TGG 0.342244 2.2:-54388449 None:intergenic
ATGAGTTTCTGAAGCTTTGT+TGG 0.343120 2.2:-54388499 None:intergenic
ATTGTCTTGGGAGATGAATC+TGG 0.347852 2.2:+54387103 MS.gene058204:CDS
ACTTGTTGAGCATAATATAT+TGG 0.354013 2.2:+54387801 MS.gene058204:CDS
GTTATGCCAGAAACATTTCA+TGG 0.360799 2.2:+54387634 MS.gene058204:CDS
GGAAATAAAAGCAGAGTTGA+TGG 0.361988 2.2:+54386827 MS.gene058204:CDS
CGACCGCTGGGAGTATTTGT+TGG 0.367452 2.2:+54387442 MS.gene058204:CDS
GATATTCTTCATATTCCTCT+TGG 0.370740 2.2:-54388140 None:intergenic
AGAAATTAGAATGGGATGTT+TGG 0.393590 2.2:+54386937 MS.gene058204:CDS
TATAGTAAAAGAATTGGATT+TGG 0.393932 2.2:+54386788 MS.gene058204:CDS
TGCGTAAAAGATTTGAAAGA+AGG 0.394307 2.2:+54388171 MS.gene058204:CDS
CTGTATTCCACCAGCTTGCT+TGG 0.394330 2.2:-54387158 None:intergenic
AAAGAGTTCACAATTCGGTT+TGG 0.397902 2.2:+54387714 MS.gene058204:CDS
CACAAATGATAAATGATTAT+GGG 0.400438 2.2:+54387365 MS.gene058204:CDS
TTACACTCCTAGAATATTTG+AGG 0.404149 2.2:+54388113 MS.gene058204:CDS
TCAAATAACCACTTGAAAGA+AGG 0.406075 2.2:-54387526 None:intergenic
GCTGGACTCTTCATAAACTT+TGG 0.408472 2.2:-54388541 None:intergenic
AGGAAACAGTCACTAAAGTA+TGG 0.410122 2.2:+54387229 MS.gene058204:CDS
AGTGTCTTGATCCGTCAAAA+TGG 0.423186 2.2:-54387587 None:intergenic
AATTTAAATCAGACTTCAAA+TGG 0.428063 2.2:-54387951 None:intergenic
TGGTCGGAGACGGCGGCGGC+GGG 0.433188 2.2:-54386152 None:intergenic
ACCACATGCAAAACAAATAA+GGG 0.433258 2.2:+54387415 MS.gene058204:CDS
ATATAAGACAGAATGCTTTA+CGG 0.434726 2.2:+54387671 MS.gene058204:CDS
CGAATTACCTTGAAACCAAA+AGG 0.436415 2.2:+54387209 MS.gene058204:CDS
GCATACAATTTATCAATTGA+AGG 0.436807 2.2:+54387837 MS.gene058204:CDS
GTTCTATTATGACTTTCAAA+TGG 0.442664 2.2:+54387303 MS.gene058204:CDS
ATTGTACCGCTTCATGAAAT+GGG 0.445421 2.2:-54388448 None:intergenic
AACAACAATGGCAGCGGCGC+AGG 0.452252 2.2:-54386177 None:intergenic
AATGGGATGTTTGGCGCGTA+TGG 0.453226 2.2:+54386946 MS.gene058204:CDS
TATCAAAAGAGTTCACAATT+CGG 0.453232 2.2:+54387709 MS.gene058204:CDS
GACAAAAGACGTCAACCAAA+AGG 0.461065 2.2:+54386902 MS.gene058204:CDS
TGAAACATTTCTCAAGGCAA+TGG 0.465247 2.2:+54387546 MS.gene058204:CDS
GAAACATTTCTCAAGGCAAT+GGG 0.465404 2.2:+54387547 MS.gene058204:CDS
TAGTGTTGGTAGCAGGATGA+TGG 0.465525 2.2:-54386124 None:intergenic
CCAACGGAGAGGATAGTTAT+GGG 0.468436 2.2:+54388222 MS.gene058204:CDS
GGTTCGTTAGTGTTGGTAGC+AGG 0.468658 2.2:-54386131 None:intergenic
CAAATAACCACTTGAAAGAA+GGG 0.471210 2.2:-54387525 None:intergenic
CCCATAACTATCCTCTCCGT+TGG 0.473881 2.2:-54388222 None:intergenic
TCCAACGGAGAGGATAGTTA+TGG 0.475548 2.2:+54388221 MS.gene058204:CDS
GCTGCCATTGTTGTTGATCA+AGG 0.480338 2.2:+54386185 MS.gene058204:CDS
GGTTGGGAAGTGATCAAGAT+TGG 0.482271 2.2:+54388394 MS.gene058204:CDS
CAAAATAATTGATGATATGT+TGG 0.486221 2.2:+54388563 MS.gene058204:CDS
TTGGTTAACTTAACCATTCG+AGG 0.487402 2.2:-54388480 None:intergenic
CCCATTCTAATTTCTGCTCT+AGG 0.487681 2.2:-54386929 None:intergenic
AGTGTTGGTAGCAGGATGAT+GGG 0.490017 2.2:-54386123 None:intergenic
GCTGGTGGAATACAGATAGT+TGG 0.495710 2.2:+54387166 MS.gene058204:CDS
ACAAAAGACGTCAACCAAAA+GGG 0.496414 2.2:+54386903 MS.gene058204:CDS
GATAGTTATGGGAAATGCTA+TGG 0.498511 2.2:+54388233 MS.gene058204:CDS
TGCCAACAATGTACCTCACT+TGG 0.498928 2.2:+54386718 MS.gene058204:CDS
TTCCAAGTGAGGTACATTGT+TGG 0.502644 2.2:-54386720 None:intergenic
AAATAAGGGTTATCGACCGC+TGG 0.503228 2.2:+54387429 MS.gene058204:CDS
ACAAGCATCACAAATTGTCT+TGG 0.503759 2.2:+54387090 MS.gene058204:CDS
TGGTTTCAAGGTAATTCGTA+AGG 0.504744 2.2:-54387204 None:intergenic
CGAATCATATGAACATGCTC+AGG 0.508001 2.2:-54387043 None:intergenic
GGCAGCGGCGCAGGGATGGT+CGG 0.512257 2.2:-54386168 None:intergenic
TGGACAAGAAATGCAAGGTT+GGG 0.513978 2.2:+54388378 MS.gene058204:CDS
ATGGACAAGAAATGCAAGGT+TGG 0.517510 2.2:+54388377 MS.gene058204:CDS
GGGATGGTCGGAGACGGCGG+CGG 0.517961 2.2:-54386156 None:intergenic
CAAGCATCACAAATTGTCTT+GGG 0.518135 2.2:+54387091 MS.gene058204:CDS
GTTATTTGAAACATTTCTCA+AGG 0.522402 2.2:+54387540 MS.gene058204:CDS
GGAAATGAAGTTTGATTCTG+AGG 0.525153 2.2:+54386739 MS.gene058204:CDS
GTTGAGCATAATATATTGGA+AGG 0.525933 2.2:+54387805 MS.gene058204:CDS
CAATGGCAGCGGCGCAGGGA+TGG 0.526019 2.2:-54386172 None:intergenic
GCCTAGAGCAGAAATTAGAA+TGG 0.527171 2.2:+54386928 MS.gene058204:CDS
GGCGCAGGGATGGTCGGAGA+CGG 0.533543 2.2:-54386162 None:intergenic
AATGGGCATGGACATATGTC+CGG 0.534093 2.2:+54387863 MS.gene058204:CDS
AAACAATCCCTTCTTTCAAG+TGG 0.538998 2.2:+54387518 MS.gene058204:CDS
GGCGGCGGGTTCGTTAGTGT+TGG 0.541693 2.2:-54386138 None:intergenic
GAAGTTTGATTCTGAGGCAG+CGG 0.545649 2.2:+54386745 MS.gene058204:CDS
GCCTAGATTGAAAATGAAGA+AGG 0.546195 2.2:+54388062 MS.gene058204:CDS
TCAAGACACTGCGATGGCTA+AGG 0.547319 2.2:+54387600 MS.gene058204:CDS
GCGACACAATCAAGTCAAGT+TGG 0.547894 2.2:+54388933 MS.gene058204:CDS
GTCACTAAAGTATGGTGAGG+TGG 0.552933 2.2:+54387237 MS.gene058204:CDS
GAAGTGATCAAGATTGGAAA+GGG 0.552959 2.2:+54388400 MS.gene058204:CDS
GTCCGGAAAACATTCACTGC+AGG 0.554763 2.2:+54387880 MS.gene058204:CDS
GCAGGGATGGTCGGAGACGG+CGG 0.559162 2.2:-54386159 None:intergenic
TCACTAAAGTATGGTGAGGT+GGG 0.560370 2.2:+54387238 MS.gene058204:CDS
TTGTTGGACTAAATAATCAC+AGG 0.567844 2.2:+54387458 MS.gene058204:CDS
CCTAGAGCAGAAATTAGAAT+GGG 0.568736 2.2:+54386929 MS.gene058204:CDS
ACAACAATGGCAGCGGCGCA+GGG 0.575609 2.2:-54386176 None:intergenic
CAAAATGTGCAACACGGACA+AGG 0.581561 2.2:+54386689 MS.gene058204:CDS
GTGTTGGTAGCAGGATGATG+GGG 0.582430 2.2:-54386122 None:intergenic
GGAAGTGATCAAGATTGGAA+AGG 0.584639 2.2:+54388399 MS.gene058204:CDS
GTATGGTTATTTCACTCGAT+CGG 0.586251 2.2:+54386963 MS.gene058204:CDS
TATATATCCAAGCAAGCTGG+TGG 0.588298 2.2:+54387151 MS.gene058204:CDS
ATAATATATTGGAAGGGTCG+TGG 0.588775 2.2:+54387812 MS.gene058204:CDS
CAATGAGTTATGTCCTCGAA+TGG 0.589590 2.2:+54388467 MS.gene058204:CDS
GAATATATATCCAAGCAAGC+TGG 0.590655 2.2:+54387148 MS.gene058204:CDS
AAACCTATATACTTAAGCGA+TGG 0.590891 2.2:+54388358 MS.gene058204:CDS
GATAGATGTACACGAAGATG+AGG 0.599215 2.2:+54387750 MS.gene058204:CDS
CATGCTCAGGTTGAAGTTGG+TGG 0.600927 2.2:-54387030 None:intergenic
AATAAGGGTTATCGACCGCT+GGG 0.601235 2.2:+54387430 MS.gene058204:CDS
TCAAACTTCATTTCCAAGTG+AGG 0.602359 2.2:-54386731 None:intergenic
CAAAAGACGTCAACCAAAAG+GGG 0.607006 2.2:+54386904 MS.gene058204:CDS
GAACATGCTCAGGTTGAAGT+TGG 0.607804 2.2:-54387033 None:intergenic
TAAAAGCCCATTTCATGAAG+CGG 0.608976 2.2:+54388442 MS.gene058204:CDS
TGTCGTAAATTTGAGACACA+TGG 0.610330 2.2:+54388276 MS.gene058204:CDS
CAATCAAGTCAAGTTGGCAA+TGG 0.617634 2.2:+54388939 MS.gene058204:CDS
AAGATTCACATGCTACAAAG+AGG 0.619398 2.2:+54386865 MS.gene058204:CDS
TTGAGCATAATATATTGGAA+GGG 0.622690 2.2:+54387806 MS.gene058204:CDS
TTGATCAACAACAATGGCAG+CGG 0.627743 2.2:-54386183 None:intergenic
GTGATCAAGATTGGAAAGGG+AGG 0.630069 2.2:+54388403 MS.gene058204:CDS
AGTATGTGGATGGAGAATCA+GGG 0.630721 2.2:+54388592 MS.gene058204:CDS
GACGGATCAAGACACTGCGA+TGG 0.636401 2.2:+54387594 MS.gene058204:CDS
TGCTACAAAGAGGGTGTACG+AGG 0.638548 2.2:+54386875 MS.gene058204:CDS
AGCGATGGACAAGAAATGCA+AGG 0.640628 2.2:+54388373 MS.gene058204:CDS
ACATTTCTCAAGGCAATGGG+TGG 0.644607 2.2:+54387550 MS.gene058204:CDS
GTATGTGGATGGAGAATCAG+GGG 0.652149 2.2:+54388593 MS.gene058204:CDS
TGAAAGTCATAATAGAACGA+GGG 0.653626 2.2:-54387298 None:intergenic
AGATTCACATGCTACAAAGA+GGG 0.654496 2.2:+54386866 MS.gene058204:CDS
ATAGATGTACACGAAGATGA+GGG 0.654743 2.2:+54387751 MS.gene058204:CDS
CACAAACTATGATAATCCAA+CGG 0.657053 2.2:+54388206 MS.gene058204:CDS
CATGAACAAAATGTGCAACA+CGG 0.658217 2.2:+54386683 MS.gene058204:CDS
ATTACCTTGATCAACAACAA+TGG 0.673823 2.2:-54386189 None:intergenic
ACAGTCACTAAAGTATGGTG+AGG 0.682809 2.2:+54387234 MS.gene058204:CDS
AATATTTGAGGAGTTCCAAG+AGG 0.693235 2.2:+54388125 MS.gene058204:CDS
TTGAAAGTCATAATAGAACG+AGG 0.700448 2.2:-54387299 None:intergenic
ACTATGATAATCCAACGGAG+AGG 0.733483 2.2:+54388211 MS.gene058204:CDS
AGTCCAACAAATACTCCCAG+CGG 0.747809 2.2:-54387445 None:intergenic
TATGTGGATGGAGAATCAGG+GGG 0.769238 2.2:+54388594 MS.gene058204:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACTATTAAAAAAACATT+GGG - chr2.2:54386392-54386411 None:intergenic 10.0%
!! GAAAAGATAAATAAATAATA+TGG - chr2.2:54388882-54388901 None:intergenic 10.0%
!! TATTACTATTAAAAAAACAT+TGG - chr2.2:54386393-54386412 None:intergenic 10.0%
!! AAAACCAAAATTTACAATTA+AGG - chr2.2:54386629-54386648 None:intergenic 15.0%
!! TTACTATTAAAAAAACATTG+GGG - chr2.2:54386391-54386410 None:intergenic 15.0%
!!! AGCAAATAACAAATATATTT+TGG + chr2.2:54387335-54387354 MS.gene058204:CDS 15.0%
!!! GCAAATAACAAATATATTTT+GGG + chr2.2:54387336-54387355 MS.gene058204:CDS 15.0%
!!! TATACATATATATAGCTTTA+TGG + chr2.2:54386327-54386346 MS.gene058204:intron 15.0%
!! AATGAGTATAGTAAAAGAAT+TGG + chr2.2:54386782-54386801 MS.gene058204:CDS 20.0%
!! AATTTAAATCAGACTTCAAA+TGG - chr2.2:54387954-54387973 None:intergenic 20.0%
!! ATGAAATTTAATTGACAATG+TGG + chr2.2:54388649-54388668 MS.gene058204:intron 20.0%
!! CACAAATGATAAATGATTAT+GGG + chr2.2:54387365-54387384 MS.gene058204:CDS 20.0%
!! TACTATTAAAAAAACATTGG+GGG - chr2.2:54386390-54386409 None:intergenic 20.0%
!! TATAGTAAAAGAATTGGATT+TGG + chr2.2:54386788-54386807 MS.gene058204:CDS 20.0%
!! TTATCAATTGAAGGAAAAAT+GGG + chr2.2:54387846-54387865 MS.gene058204:CDS 20.0%
!! TTTATCAATTGAAGGAAAAA+TGG + chr2.2:54387845-54387864 MS.gene058204:CDS 20.0%
!!! AATAACAAATATATTTTGGG+CGG + chr2.2:54387339-54387358 MS.gene058204:CDS 20.0%
!!! AATAGTAATAGATAGCTTTA+TGG + chr2.2:54386403-54386422 MS.gene058204:intron 20.0%
!!! ATAAATGATTATGGGTATTT+TGG + chr2.2:54387373-54387392 MS.gene058204:CDS 20.0%
!!! ATATATTCATGCAAATCTTT+TGG - chr2.2:54387136-54387155 None:intergenic 20.0%
!!! CAAAATAATTGATGATATGT+TGG + chr2.2:54388563-54388582 MS.gene058204:CDS 20.0%
!!! TTGATGAAAAATTAGTTTCT+AGG + chr2.2:54386275-54386294 MS.gene058204:intron 20.0%
!!! TTTGAAGTCTGATTTAAATT+TGG + chr2.2:54387954-54387973 MS.gene058204:CDS 20.0%
! AACATCATGTAATAAAGAGA+CGG - chr2.2:54386244-54386263 None:intergenic 25.0%
! ATATAAGACAGAATGCTTTA+CGG + chr2.2:54387671-54387690 MS.gene058204:CDS 25.0%
! CATAGATAAAAACTACTTAC+CGG - chr2.2:54388824-54388843 None:intergenic 25.0%
! GCACAAATGATAAATGATTA+TGG + chr2.2:54387364-54387383 MS.gene058204:CDS 25.0%
! GCATACAATTTATCAATTGA+AGG + chr2.2:54387837-54387856 MS.gene058204:CDS 25.0%
! GGCAAATAATAAAATAGAGA+AGG - chr2.2:54386223-54386242 None:intergenic 25.0%
! GTTCTATTATGACTTTCAAA+TGG + chr2.2:54387303-54387322 MS.gene058204:CDS 25.0%
! TAAATAATATGGTTTCCTAC+CGG - chr2.2:54388871-54388890 None:intergenic 25.0%
! TATCAAAAGAGTTCACAATT+CGG + chr2.2:54387709-54387728 MS.gene058204:CDS 25.0%
! TTCAGATTGTGTCAATAAAT+TGG + chr2.2:54386649-54386668 MS.gene058204:intron 25.0%
! TTCATCATTTGCAATTGATA+TGG - chr2.2:54388634-54388653 None:intergenic 25.0%
! TTGAGCATAATATATTGGAA+GGG + chr2.2:54387806-54387825 MS.gene058204:CDS 25.0%
!! ACTTGTTGAGCATAATATAT+TGG + chr2.2:54387801-54387820 MS.gene058204:CDS 25.0%
!! GTTATTTGAAACATTTCTCA+AGG + chr2.2:54387540-54387559 MS.gene058204:CDS 25.0%
!!! CATATCATCAATTATTTTGC+TGG - chr2.2:54388562-54388581 None:intergenic 25.0%
AAACCTATATACTTAAGCGA+TGG + chr2.2:54388358-54388377 MS.gene058204:CDS 30.0%
AAATTTAGTACAGTCGTTAC+CGG + chr2.2:54388802-54388821 MS.gene058204:intron 30.0%
ACCACATGCAAAACAAATAA+GGG + chr2.2:54387415-54387434 MS.gene058204:CDS 30.0%
AGAAATTAGAATGGGATGTT+TGG + chr2.2:54386937-54386956 MS.gene058204:CDS 30.0%
ATTACCTTGATCAACAACAA+TGG - chr2.2:54386192-54386211 None:intergenic 30.0%
CAAATAACCACTTGAAAGAA+GGG - chr2.2:54387528-54387547 None:intergenic 30.0%
CACAAACTATGATAATCCAA+CGG + chr2.2:54388206-54388225 MS.gene058204:CDS 30.0%
GATATTCTTCATATTCCTCT+TGG - chr2.2:54388143-54388162 None:intergenic 30.0%
GGAAAAAGCGATATAAATCT+TGG + chr2.2:54388723-54388742 MS.gene058204:intron 30.0%
GTTGAGCATAATATATTGGA+AGG + chr2.2:54387805-54387824 MS.gene058204:CDS 30.0%
TCAAATAACCACTTGAAAGA+AGG - chr2.2:54387529-54387548 None:intergenic 30.0%
TGAAAGTCATAATAGAACGA+GGG - chr2.2:54387301-54387320 None:intergenic 30.0%
TGCGTAAAAGATTTGAAAGA+AGG + chr2.2:54388171-54388190 MS.gene058204:CDS 30.0%
TTAAAAAAACATTGGGGGAA+AGG - chr2.2:54386385-54386404 None:intergenic 30.0%
TTACACTCCTAGAATATTTG+AGG + chr2.2:54388113-54388132 MS.gene058204:CDS 30.0%
TTGAAAGTCATAATAGAACG+AGG - chr2.2:54387302-54387321 None:intergenic 30.0%
TTGTTGGACTAAATAATCAC+AGG + chr2.2:54387458-54387477 MS.gene058204:CDS 30.0%
! AAAGCCAAAAACCATTTTGA+CGG + chr2.2:54387576-54387595 MS.gene058204:CDS 30.0%
! ATGTCTGAAATCATCACTTA+TGG + chr2.2:54386600-54386619 MS.gene058204:intron 30.0%
! GTGAACTCTTTTGATATAAG+TGG - chr2.2:54387705-54387724 None:intergenic 30.0%
!! AATTTTAGTACAGTCGTTAC+CGG + chr2.2:54388849-54388868 MS.gene058204:intron 30.0%
!! GTGGCCTTAATTGTAAATTT+TGG + chr2.2:54386622-54386641 MS.gene058204:intron 30.0%
AAACAATCCCTTCTTTCAAG+TGG + chr2.2:54387518-54387537 MS.gene058204:CDS 35.0%
AAAGAGTTCACAATTCGGTT+TGG + chr2.2:54387714-54387733 MS.gene058204:CDS 35.0%
AAGATTCACATGCTACAAAG+AGG + chr2.2:54386865-54386884 MS.gene058204:CDS 35.0%
AATATTTGAGGAGTTCCAAG+AGG + chr2.2:54388125-54388144 MS.gene058204:CDS 35.0%
AATTGAAGGAAAAATGGGCA+TGG + chr2.2:54387851-54387870 MS.gene058204:CDS 35.0%
ACAAAAGACGTCAACCAAAA+GGG + chr2.2:54386903-54386922 MS.gene058204:CDS 35.0%
ACAAGCATCACAAATTGTCT+TGG + chr2.2:54387090-54387109 MS.gene058204:CDS 35.0%
AGATTCACATGCTACAAAGA+GGG + chr2.2:54386866-54386885 MS.gene058204:CDS 35.0%
AGGAAACAGTCACTAAAGTA+TGG + chr2.2:54387229-54387248 MS.gene058204:CDS 35.0%
ATAATATATTGGAAGGGTCG+TGG + chr2.2:54387812-54387831 MS.gene058204:CDS 35.0%
ATAGATGTACACGAAGATGA+GGG + chr2.2:54387751-54387770 MS.gene058204:CDS 35.0%
ATTGTACCGCTTCATGAAAT+GGG - chr2.2:54388451-54388470 None:intergenic 35.0%
CAAGCATCACAAATTGTCTT+GGG + chr2.2:54387091-54387110 MS.gene058204:CDS 35.0%
CACAATCCATGAAATGTTTC+TGG - chr2.2:54387643-54387662 None:intergenic 35.0%
CACCACATGCAAAACAAATA+AGG + chr2.2:54387414-54387433 MS.gene058204:CDS 35.0%
CATGAACAAAATGTGCAACA+CGG + chr2.2:54386683-54386702 MS.gene058204:CDS 35.0%
CCTAGAGCAGAAATTAGAAT+GGG + chr2.2:54386929-54386948 MS.gene058204:CDS 35.0%
CGAATTACCTTGAAACCAAA+AGG + chr2.2:54387209-54387228 MS.gene058204:CDS 35.0%
CGCTTAAGTATATAGGTTTC+AGG - chr2.2:54388357-54388376 None:intergenic 35.0%
GAAACATTTCTCAAGGCAAT+GGG + chr2.2:54387547-54387566 MS.gene058204:CDS 35.0%
GAAGTGATCAAGATTGGAAA+GGG + chr2.2:54388400-54388419 MS.gene058204:CDS 35.0%
GAATATATATCCAAGCAAGC+TGG + chr2.2:54387148-54387167 MS.gene058204:CDS 35.0%
GATAGTTATGGGAAATGCTA+TGG + chr2.2:54388233-54388252 MS.gene058204:CDS 35.0%
GCCTAGATTGAAAATGAAGA+AGG + chr2.2:54388062-54388081 MS.gene058204:CDS 35.0%
GGAAATAAAAGCAGAGTTGA+TGG + chr2.2:54386827-54386846 MS.gene058204:CDS 35.0%
GGAAATGAAGTTTGATTCTG+AGG + chr2.2:54386739-54386758 MS.gene058204:CDS 35.0%
GTTATGCCAGAAACATTTCA+TGG + chr2.2:54387634-54387653 MS.gene058204:CDS 35.0%
TAAAAGCCCATTTCATGAAG+CGG + chr2.2:54388442-54388461 MS.gene058204:CDS 35.0%
TCAAACTTCATTTCCAAGTG+AGG - chr2.2:54386734-54386753 None:intergenic 35.0%
TGAAACATTTCTCAAGGCAA+TGG + chr2.2:54387546-54387565 MS.gene058204:CDS 35.0%
TGGAACTCCTCAAATATTCT+AGG - chr2.2:54388123-54388142 None:intergenic 35.0%
TGGTTTCAAGGTAATTCGTA+AGG - chr2.2:54387207-54387226 None:intergenic 35.0%
TGTCCATCGCTTAAGTATAT+AGG - chr2.2:54388364-54388383 None:intergenic 35.0%
TTGGTTAACTTAACCATTCG+AGG - chr2.2:54388483-54388502 None:intergenic 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr2.2:54386806-54386825 MS.gene058204:CDS 35.0%
! AGGGTGAATTTTTGAATGCT+TGG + chr2.2:54387770-54387789 MS.gene058204:CDS 35.0%
! CTTTAGTGACTGTTTCCTTT+TGG - chr2.2:54387227-54387246 None:intergenic 35.0%
! GCCTTCTTCATTTTCAATCT+AGG - chr2.2:54388066-54388085 None:intergenic 35.0%
! GTATGGTTATTTCACTCGAT+CGG + chr2.2:54386963-54386982 MS.gene058204:CDS 35.0%
! TATGTTGGCCAAAAAGTATG+TGG + chr2.2:54388578-54388597 MS.gene058204:CDS 35.0%
! TCTGAAATCATCACTTATGG+TGG + chr2.2:54386603-54386622 MS.gene058204:intron 35.0%
! TGATCCGTCAAAATGGTTTT+TGG - chr2.2:54387583-54387602 None:intergenic 35.0%
! TGCAGTCTAAGTTTTTCTAG+TGG + chr2.2:54386440-54386459 MS.gene058204:intron 35.0%
! TGTCGTAAATTTGAGACACA+TGG + chr2.2:54388276-54388295 MS.gene058204:CDS 35.0%
! TTCTCCATCCACATACTTTT+TGG - chr2.2:54388589-54388608 None:intergenic 35.0%
!! AAAACAACATTCTGAAGTCG+CGG - chr2.2:54386548-54386567 None:intergenic 35.0%
!! ATGAGTTTCTGAAGCTTTGT+TGG - chr2.2:54388502-54388521 None:intergenic 35.0%
!!! ACCCTTATTTGTTTTGCATG+TGG - chr2.2:54387419-54387438 None:intergenic 35.0%
!!! ACTGTTTCCTTTTGGTTTCA+AGG - chr2.2:54387219-54387238 None:intergenic 35.0%
AAAAACATTGGGGGAAAGGA+TGG - chr2.2:54386381-54386400 None:intergenic 40.0%
AAGTATGTGGATGGAGAATC+AGG + chr2.2:54388591-54388610 MS.gene058204:CDS 40.0%
ACAGTCACTAAAGTATGGTG+AGG + chr2.2:54387234-54387253 MS.gene058204:CDS 40.0%
ACTATGATAATCCAACGGAG+AGG + chr2.2:54388211-54388230 MS.gene058204:CDS 40.0%
AGTATGTGGATGGAGAATCA+GGG + chr2.2:54388592-54388611 MS.gene058204:CDS 40.0%
AGTGTCTTGATCCGTCAAAA+TGG - chr2.2:54387590-54387609 None:intergenic 40.0%
ATGGACAAGAAATGCAAGGT+TGG + chr2.2:54388377-54388396 MS.gene058204:CDS 40.0%
ATTGTCTTGGGAGATGAATC+TGG + chr2.2:54387103-54387122 MS.gene058204:CDS 40.0%
CAAAAGACGTCAACCAAAAG+GGG + chr2.2:54386904-54386923 MS.gene058204:CDS 40.0%
CAATGAGTTATGTCCTCGAA+TGG + chr2.2:54388467-54388486 MS.gene058204:CDS 40.0%
CATTGTACCGCTTCATGAAA+TGG - chr2.2:54388452-54388471 None:intergenic 40.0%
CCCATTCTAATTTCTGCTCT+AGG - chr2.2:54386932-54386951 None:intergenic 40.0%
CGAATCATATGAACATGCTC+AGG - chr2.2:54387046-54387065 None:intergenic 40.0%
GACAAAAGACGTCAACCAAA+AGG + chr2.2:54386902-54386921 MS.gene058204:CDS 40.0%
GATAGATGTACACGAAGATG+AGG + chr2.2:54387750-54387769 MS.gene058204:CDS 40.0%
GCCTAGAGCAGAAATTAGAA+TGG + chr2.2:54386928-54386947 MS.gene058204:CDS 40.0%
GCTGGACTCTTCATAAACTT+TGG - chr2.2:54388544-54388563 None:intergenic 40.0%
GGAAGTGATCAAGATTGGAA+AGG + chr2.2:54388399-54388418 MS.gene058204:CDS 40.0%
TATATATCCAAGCAAGCTGG+TGG + chr2.2:54387151-54387170 MS.gene058204:CDS 40.0%
TCACTAAAGTATGGTGAGGT+GGG + chr2.2:54387238-54387257 MS.gene058204:CDS 40.0%
TGGACAAGAAATGCAAGGTT+GGG + chr2.2:54388378-54388397 MS.gene058204:CDS 40.0%
TTCACCAGATCTCTTTCTCT+TGG - chr2.2:54388783-54388802 None:intergenic 40.0%
TTCCAAGTGAGGTACATTGT+TGG - chr2.2:54386723-54386742 None:intergenic 40.0%
TTGATCAACAACAATGGCAG+CGG - chr2.2:54386186-54386205 None:intergenic 40.0%
! CATTTCATGGATTGTGCACT+TGG + chr2.2:54387647-54387666 MS.gene058204:CDS 40.0%
! TACCTGCAGTGAATGTTTTC+CGG - chr2.2:54387885-54387904 None:intergenic 40.0%
! TATGCCTTTTGCCATACCAA+CGG - chr2.2:54388678-54388697 None:intergenic 40.0%
! TTGGCCAAAAAGTATGTGGA+TGG + chr2.2:54388582-54388601 MS.gene058204:CDS 40.0%
!! CAATCAAGTCAAGTTGGCAA+TGG + chr2.2:54388939-54388958 MS.gene058204:CDS 40.0%
!! CACAGGCAAACAGTCATTTT+TGG + chr2.2:54387475-54387494 MS.gene058204:CDS 40.0%
AAATAAGGGTTATCGACCGC+TGG + chr2.2:54387429-54387448 MS.gene058204:CDS 45.0%
AATAAGGGTTATCGACCGCT+GGG + chr2.2:54387430-54387449 MS.gene058204:CDS 45.0%
AATGGGCATGGACATATGTC+CGG + chr2.2:54387863-54387882 MS.gene058204:CDS 45.0%
ACATTTCTCAAGGCAATGGG+TGG + chr2.2:54387550-54387569 MS.gene058204:CDS 45.0%
AGCGATGGACAAGAAATGCA+AGG + chr2.2:54388373-54388392 MS.gene058204:CDS 45.0%
AGTCCAACAAATACTCCCAG+CGG - chr2.2:54387448-54387467 None:intergenic 45.0%
ATCGAGAAAGTTCACGCAAG+CGG + chr2.2:54388702-54388721 MS.gene058204:intron 45.0%
ATTGACAATGTGGACACCGT+TGG + chr2.2:54388659-54388678 MS.gene058204:intron 45.0%
CAAAATGTGCAACACGGACA+AGG + chr2.2:54386689-54386708 MS.gene058204:CDS 45.0%
CCAACGGAGAGGATAGTTAT+GGG + chr2.2:54388222-54388241 MS.gene058204:CDS 45.0%
GCGACACAATCAAGTCAAGT+TGG + chr2.2:54388933-54388952 MS.gene058204:CDS 45.0%
GCTGGTGGAATACAGATAGT+TGG + chr2.2:54387166-54387185 MS.gene058204:CDS 45.0%
GGTTGGGAAGTGATCAAGAT+TGG + chr2.2:54388394-54388413 MS.gene058204:CDS 45.0%
GTATGTGGATGGAGAATCAG+GGG + chr2.2:54388593-54388612 MS.gene058204:CDS 45.0%
GTCACTAAAGTATGGTGAGG+TGG + chr2.2:54387237-54387256 MS.gene058204:CDS 45.0%
GTGATCAAGATTGGAAAGGG+AGG + chr2.2:54388403-54388422 MS.gene058204:CDS 45.0%
TATGTGGATGGAGAATCAGG+GGG + chr2.2:54388594-54388613 MS.gene058204:CDS 45.0%
TCCAACGGAGAGGATAGTTA+TGG + chr2.2:54388221-54388240 MS.gene058204:CDS 45.0%
TGCCAACAATGTACCTCACT+TGG + chr2.2:54386718-54386737 MS.gene058204:CDS 45.0%
TGCTCCAAGAGAAAGAGATC+TGG + chr2.2:54388776-54388795 MS.gene058204:intron 45.0%
TTAGTACAGTCGTTACCGGT+AGG + chr2.2:54388806-54388825 MS.gene058204:intron 45.0%
! GCTGCCATTGTTGTTGATCA+AGG + chr2.2:54386185-54386204 MS.gene058204:CDS 45.0%
!! AGTGTTGGTAGCAGGATGAT+GGG - chr2.2:54386126-54386145 None:intergenic 45.0%
!! GAACATGCTCAGGTTGAAGT+TGG - chr2.2:54387036-54387055 None:intergenic 45.0%
!! GAAGTTTGATTCTGAGGCAG+CGG + chr2.2:54386745-54386764 MS.gene058204:CDS 45.0%
!! TAGTGTTGGTAGCAGGATGA+TGG - chr2.2:54386127-54386146 None:intergenic 45.0%
AATGGGATGTTTGGCGCGTA+TGG + chr2.2:54386946-54386965 MS.gene058204:CDS 50.0%
CAATGTGGACACCGTTGGTA+TGG + chr2.2:54388664-54388683 MS.gene058204:intron 50.0%
CCCATAACTATCCTCTCCGT+TGG - chr2.2:54388225-54388244 None:intergenic 50.0%
GACACCGTTGGTATGGCAAA+AGG + chr2.2:54388671-54388690 MS.gene058204:intron 50.0%
GTCCGGAAAACATTCACTGC+AGG + chr2.2:54387880-54387899 MS.gene058204:CDS 50.0%
TCAAGACACTGCGATGGCTA+AGG + chr2.2:54387600-54387619 MS.gene058204:CDS 50.0%
! CTGTATTCCACCAGCTTGCT+TGG - chr2.2:54387161-54387180 None:intergenic 50.0%
! TGCTACAAAGAGGGTGTACG+AGG + chr2.2:54386875-54386894 MS.gene058204:CDS 50.0%
!! CATGCTCAGGTTGAAGTTGG+TGG - chr2.2:54387033-54387052 None:intergenic 50.0%
!! GGTTCGTTAGTGTTGGTAGC+AGG - chr2.2:54386134-54386153 None:intergenic 50.0%
!! GTGTTGGTAGCAGGATGATG+GGG - chr2.2:54386125-54386144 None:intergenic 50.0%
GACGGATCAAGACACTGCGA+TGG + chr2.2:54387594-54387613 MS.gene058204:CDS 55.0%
!! CGACCGCTGGGAGTATTTGT+TGG + chr2.2:54387442-54387461 MS.gene058204:CDS 55.0%
!! TCTGCTCTAGGCTCCCCTTT+TGG - chr2.2:54386920-54386939 None:intergenic 55.0%
AACAACAATGGCAGCGGCGC+AGG - chr2.2:54386180-54386199 None:intergenic 60.0%
ACAACAATGGCAGCGGCGCA+GGG - chr2.2:54386179-54386198 None:intergenic 60.0%
GGATGGAGAATCAGGGGGCA+TGG + chr2.2:54388599-54388618 MS.gene058204:CDS 60.0%
! GGCGGCGGGTTCGTTAGTGT+TGG - chr2.2:54386141-54386160 None:intergenic 65.0%
CAATGGCAGCGGCGCAGGGA+TGG - chr2.2:54386175-54386194 None:intergenic 70.0%
GCAGGGATGGTCGGAGACGG+CGG - chr2.2:54386162-54386181 None:intergenic 70.0%
GGCGCAGGGATGGTCGGAGA+CGG - chr2.2:54386165-54386184 None:intergenic 70.0%
ATGGTCGGAGACGGCGGCGG+CGG - chr2.2:54386156-54386175 None:intergenic 75.0%
GGCAGCGGCGCAGGGATGGT+CGG - chr2.2:54386171-54386190 None:intergenic 75.0%
GGGATGGTCGGAGACGGCGG+CGG - chr2.2:54386159-54386178 None:intergenic 75.0%
! TGGTCGGAGACGGCGGCGGC+GGG - chr2.2:54386155-54386174 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 54386113 54388995 54386113 ID=MS.gene058204
chr2.2 mRNA 54386113 54388995 54386113 ID=MS.gene058204.t1;Parent=MS.gene058204
chr2.2 exon 54386113 54386206 54386113 ID=MS.gene058204.t1.exon1;Parent=MS.gene058204.t1
chr2.2 CDS 54386113 54386206 54386113 ID=cds.MS.gene058204.t1;Parent=MS.gene058204.t1
chr2.2 exon 54386654 54388626 54386654 ID=MS.gene058204.t1.exon2;Parent=MS.gene058204.t1
chr2.2 CDS 54386654 54388626 54386654 ID=cds.MS.gene058204.t1;Parent=MS.gene058204.t1
chr2.2 exon 54388915 54388995 54388915 ID=MS.gene058204.t1.exon3;Parent=MS.gene058204.t1
chr2.2 CDS 54388915 54388995 54388915 ID=cds.MS.gene058204.t1;Parent=MS.gene058204.t1
Gene Sequence

>MS.gene058204

ATGAGCGAACCCCATCATCCTGCTACCAACACTAACGAACCCGCCGCCGCCGTCTCCGACCATCCCTGCGCCGCTGCCATTGTTGTTGATCAAGGTAATATAGTTTGCCTTCTCTATTTTATTATTTGCCGTCTCTTTATTACATGATGTTGTTTTACTGTGTTGATGAAAAATTAGTTTCTAGGTTCACTTACGTGATGCATTACTTACATGATATACATATATATAGCTTTATGGTCACTATGTGTGTAACTTTATATAATCACCATCCTTTCCCCCAATGTTTTTTTAATAGTAATAGATAGCTTTATGGTCAACAAATTCTTATGCAGTCTAAGTTTTTCTAGTGGCTTTTTGCTGTTATGTTGTTTTTGTTCAGTTGTTGTCAAAAAATCATCAGTTGTTTCTGTTTATGTTGTTTCTGTTCAGTTGCCGCGACTTCAGAATGTTGTTTTCTAACACTGTTATCATATATTCATGTAAGTTTATGTCTGAAATCATCACTTATGGTGGCCTTAATTGTAAATTTTGGTTTTTTCAGATTGTGTCAATAAATTGGTTAACAACATTCATGAACAAAATGTGCAACACGGACAAGGAAGTATTGCCAACAATGTACCTCACTTGGAAATGAAGTTTGATTCTGAGGCAGCGGCTTATGACTTTTATAATGAGTATAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAGTTGATGGAGTTTTGACTTCAAGAAGATTCACATGCTACAAAGAGGGTGTACGAGGTGTTGACAAAAGACGTCAACCAAAAGGGGAGCCTAGAGCAGAAATTAGAATGGGATGTTTGGCGCGTATGGTTATTTCACTCGATCGGAAGATAGAGAAATATAAAGTTGTTGACTTTATAGCTGAACATAACCACCAACTTCAACCTGAGCATGTTCATATGATTCGCTCTCATCGACGCATATCTGAGACACAAGCATCACAAATTGTCTTGGGAGATGAATCTGGATTAAGACCAAAAGATTTGCATGAATATATATCCAAGCAAGCTGGTGGAATACAGATAGTTGGTTTTACAAAAAAAGTCCTTACGAATTACCTTGAAACCAAAAGGAAACAGTCACTAAAGTATGGTGAGGTGGGTGCACTGATGATGTATTTCAAAAAAGAAAGTGAAAATCCCTCGTTCTATTATGACTTTCAAATGGATGTTGAAGAGCAAATAACAAATATATTTTGGGCGGATGCACAAATGATAAATGATTATGGGTATTTTGGTGATGTTATTACTTTTGACACCACATGCAAAACAAATAAGGGTTATCGACCGCTGGGAGTATTTGTTGGACTAAATAATCACAGGCAAACAGTCATTTTTGGTGCAGCATTGTTATATGACGAAACAATCCCTTCTTTCAAGTGGTTATTTGAAACATTTCTCAAGGCAATGGGTGGTAAAAAGCCAAAAACCATTTTGACGGATCAAGACACTGCGATGGCTAAGGCTATTTCTTTAGTTATGCCAGAAACATTTCATGGATTGTGCACTTGGCATATAAGACAGAATGCTTTACGGCATGTGAACCACTTATATCAAAAGAGTTCACAATTCGGTTTGGATTTTGAAGCATTGATAGATGTACACGAAGATGAGGGTGAATTTTTGAATGCTTGGAATTCTTTACTTGTTGAGCATAATATATTGGAAGGGTCGTGGTTGCATACAATTTATCAATTGAAGGAAAAATGGGCATGGACATATGTCCGGAAAACATTCACTGCAGGTATGAGATCTACTCAATTAAGTGAGAGCTTTAATGCTGATTTGAAGAACCATTTGAAGTCTGATTTAAATTTGGTACAATTTTTTACACACTTTAAAAGAGCTGTTAATGATAAACGAAACAAAGAGTCAGAAGCTGAATATGAGTCAAAAAATAAGCTGCCTAGATTGAAAATGAAGAAGGCTCGTATGCTCATACAAGCTGCAAATGTTTACACTCCTAGAATATTTGAGGAGTTCCAAGAGGAATATGAAGAATATCAAGACACTTGCGTAAAAGATTTGAAAGAAGGTTTGTATGCTGTCACAAACTATGATAATCCAACGGAGAGGATAGTTATGGGAAATGCTATGGAACAAAAAGTTTCATGTGATTGTCGTAAATTTGAGACACATGGCATCTTATGCAGTCATGCTTTGAAAGTTTTAGATGTCATGAACAGTAAGTTAATTCCTGAAACCTATATACTTAAGCGATGGACAAGAAATGCAAGGTTGGGAAGTGATCAAGATTGGAAAGGGAGGCATGTTGAATTAGACATAAAAGCCCATTTCATGAAGCGGTACAATGAGTTATGTCCTCGAATGGTTAAGTTAACCAACAAAGCTTCAGAAACTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAATTGATGATATGTTGGCCAAAAAGTATGTGGATGGAGAATCAGGGGGCATGGTTCATGTATCCATATCAATTGCAAATGATGAAATTTAATTGACAATGTGGACACCGTTGGTATGGCAAAAGGCATAGCTAATCGAGAAAGTTCACGCAAGCGGAAAAAGCGATATAAATCTTGGATTGAGAAAAAAGATAAAAAGACAAAGATGTGCTCCAAGAGAAAGAGATCTGGTGAAAATTTAGTACAGTCGTTACCGGTAAGTAGTTTTTATCTATGTATTAAATTTTAGTACAGTCGTTACCGGTAGGAAACCATATTATTTATTTATCTTTTCTTACATTATGCAGCAAGAAACTACAGTTCAAGCGACACAATCAAGTCAAGTTGGCAATGGAACTCAACATCAAGCTACAATCCAAGAGTCATAA
Protein sequence

>MS.gene058204.t1

MSEPHHPATNTNEPAAAVSDHPCAAAIVVDQDCVNKLVNNIHEQNVQHGQGSIANNVPHLEMKFDSEAAAYDFYNEYSKRIGFGIRREYGNKSRVDGVLTSRRFTCYKEGVRGVDKRRQPKGEPRAEIRMGCLARMVISLDRKIEKYKVVDFIAEHNHQLQPEHVHMIRSHRRISETQASQIVLGDESGLRPKDLHEYISKQAGGIQIVGFTKKVLTNYLETKRKQSLKYGEVGALMMYFKKESENPSFYYDFQMDVEEQITNIFWADAQMINDYGYFGDVITFDTTCKTNKGYRPLGVFVGLNNHRQTVIFGAALLYDETIPSFKWLFETFLKAMGGKKPKTILTDQDTAMAKAISLVMPETFHGLCTWHIRQNALRHVNHLYQKSSQFGLDFEALIDVHEDEGEFLNAWNSLLVEHNILEGSWLHTIYQLKEKWAWTYVRKTFTAGMRSTQLSESFNADLKNHLKSDLNLVQFFTHFKRAVNDKRNKESEAEYESKNKLPRLKMKKARMLIQAANVYTPRIFEEFQEEYEEYQDTCVKDLKEGLYAVTNYDNPTERIVMGNAMEQKVSCDCRKFETHGILCSHALKVLDVMNSKLIPETYILKRWTRNARLGSDQDWKGRHVELDIKAHFMKRYNELCPRMVKLTNKASETHETYTFLSKVYEESSKIIDDMLAKKYVDGESGGMVHQETTVQATQSSQVGNGTQHQATIQES