AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058224

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058224.t1 MTR_2g034280 90.635 598 34 3 1 597 1 577 0.0 1090
MS.gene058224.t1 MTR_2g034250 78.974 604 103 7 1 597 1 587 0.0 953
MS.gene058224.t1 MTR_2g034260 79.700 601 103 8 1 597 1 586 0.0 950
MS.gene058224.t1 MTR_7g109580 43.701 508 266 7 91 597 68 556 3.85e-135 405
MS.gene058224.t1 MTR_1g086970 44.399 491 241 9 116 597 13 480 7.49e-129 387
MS.gene058224.t1 MTR_3g065980 28.935 432 274 8 181 596 127 541 7.48e-58 203
MS.gene058224.t1 MTR_7g027190 29.950 404 250 10 209 595 286 673 7.27e-48 178
MS.gene058224.t1 MTR_8g442410 31.701 388 247 12 216 595 148 525 5.78e-44 165
MS.gene058224.t1 MTR_7g074650 31.989 372 229 11 227 595 442 792 5.08e-42 162
MS.gene058224.t1 MTR_4g104020 28.218 404 267 11 205 595 127 520 4.53e-41 156
MS.gene058224.t1 MTR_4g076140 28.315 445 242 10 214 595 39 469 4.59e-41 155
MS.gene058224.t1 MTR_4g064150 28.354 395 257 9 213 594 351 732 8.65e-38 149
MS.gene058224.t1 MTR_7g062120 27.763 389 263 8 214 595 363 740 3.97e-37 147
MS.gene058224.t1 MTR_3g022830 27.635 427 240 8 224 596 26 437 8.46e-37 142
MS.gene058224.t1 MTR_1g069725 28.238 386 251 10 213 594 99 462 1.72e-36 142
MS.gene058224.t1 MTR_4g076020 28.205 390 211 9 227 595 88 429 3.10e-36 141
MS.gene058224.t1 MTR_4g064200 27.132 387 256 10 218 594 279 649 5.40e-36 143
MS.gene058224.t1 MTR_4g102790 27.225 382 254 11 224 594 293 661 7.11e-36 143
MS.gene058224.t1 MTR_5g094450 26.632 383 266 9 220 595 158 532 7.68e-36 141
MS.gene058224.t1 MTR_2g026250 25.974 385 267 8 218 595 223 596 4.12e-35 140
MS.gene058224.t1 MTR_4g133660 24.740 384 271 8 218 594 170 542 3.43e-34 137
MS.gene058224.t1 MTR_3g056110 26.823 384 260 9 220 595 172 542 4.68e-34 136
MS.gene058224.t1 MTR_4g064160 26.142 394 253 8 218 594 311 683 6.97e-34 137
MS.gene058224.t1 MTR_4g064120 28.165 387 252 12 212 585 243 616 9.13e-34 136
MS.gene058224.t1 MTR_4g077760 26.440 382 259 9 220 595 190 555 2.49e-33 134
MS.gene058224.t1 MTR_2g097410 26.463 393 263 8 214 594 362 740 7.58e-33 134
MS.gene058224.t1 MTR_5g097480 26.471 340 229 7 220 550 174 501 1.27e-32 132
MS.gene058224.t1 MTR_2g097463 26.396 394 249 9 218 594 285 654 2.16e-32 132
MS.gene058224.t1 MTR_2g097473 27.390 387 254 9 218 594 284 653 4.01e-32 132
MS.gene058224.t1 MTR_2g097390 24.093 386 275 7 215 594 267 640 1.72e-31 130
MS.gene058224.t1 MTR_2g097350 25.130 386 271 7 215 594 266 639 2.71e-31 129
MS.gene058224.t1 MTR_4g064180 27.532 385 257 11 212 585 243 616 2.92e-31 129
MS.gene058224.t1 MTR_3g072710 25.954 393 277 8 213 594 110 499 3.14e-31 127
MS.gene058224.t1 MTR_2g097310 24.093 386 275 7 215 594 264 637 1.22e-30 127
MS.gene058224.t1 MTR_3g089055 26.098 387 251 11 224 595 204 570 1.65e-30 126
MS.gene058224.t1 MTR_3g089055 26.098 387 251 11 224 595 199 565 1.83e-30 126
MS.gene058224.t1 MTR_2g082090 25.974 385 260 10 220 594 209 578 3.17e-30 125
MS.gene058224.t1 MTR_2g097467 25.974 385 262 8 218 594 284 653 3.26e-30 126
MS.gene058224.t1 MTR_1g029420 25.773 388 251 9 224 595 226 592 4.26e-30 125
MS.gene058224.t1 MTR_1g096030 25.837 418 261 14 208 597 65 461 6.27e-27 114
MS.gene058224.t1 MTR_4g097080 24.000 400 281 7 203 595 120 503 4.20e-26 112
MS.gene058224.t1 MTR_2g097380 24.586 362 226 10 239 594 240 560 5.59e-26 112
MS.gene058224.t1 MTR_5g009080 31.907 257 157 7 189 444 25 264 4.53e-24 106
MS.gene058224.t1 MTR_5g009080 32.237 152 101 2 445 595 328 478 2.69e-20 94.7
MS.gene058224.t1 MTR_5g058860 23.602 483 311 16 148 594 36 496 6.17e-24 105
MS.gene058224.t1 MTR_8g093070 27.068 399 248 15 210 594 126 495 6.21e-24 105
MS.gene058224.t1 MTR_5g015490 25.128 390 260 11 224 595 115 490 1.26e-23 105
MS.gene058224.t1 MTR_7g057230 25.926 405 266 13 205 594 271 656 1.84e-21 99.4
MS.gene058224.t1 MTR_4g026485 24.934 381 261 10 219 595 265 624 2.92e-21 98.6
MS.gene058224.t1 MTR_2g099110 27.682 289 192 8 224 507 75 351 1.08e-20 95.5
MS.gene058224.t1 MTR_6g047750 26.329 395 244 16 222 595 294 662 8.76e-19 90.9
MS.gene058224.t1 MTR_0092s0100 24.074 378 269 8 219 595 371 731 1.90e-17 86.7
MS.gene058224.t1 MTR_0092s0100 24.074 378 269 8 219 595 368 728 2.04e-17 86.7
MS.gene058224.t1 MTR_8g077940 25.627 359 220 14 222 562 181 510 2.15e-13 73.6
MS.gene058224.t1 MTR_8g020840 19.851 403 285 11 216 596 163 549 4.36e-13 72.4
MS.gene058224.t1 MTR_2g089100 19.764 339 219 8 224 525 53 375 4.28e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058224.t1 AT3G03450 30.886 395 247 8 210 596 170 546 3.91e-58 204
MS.gene058224.t1 AT1G66350 30.233 387 245 7 212 596 144 507 3.39e-55 195
MS.gene058224.t1 AT5G17490 31.266 387 242 9 214 596 151 517 5.59e-54 192
MS.gene058224.t1 AT1G14920 31.026 390 248 8 210 596 159 530 2.17e-53 191
MS.gene058224.t1 AT2G01570 30.256 390 251 7 210 596 211 582 3.29e-53 191
MS.gene058224.t1 AT5G41920 32.143 392 239 12 210 595 30 400 2.43e-47 171
MS.gene058224.t1 AT3G54220 31.892 370 233 8 227 594 297 649 3.44e-43 164
MS.gene058224.t1 AT1G07530 28.715 397 264 7 206 594 379 764 4.80e-40 156
MS.gene058224.t1 AT2G29060 29.114 395 249 10 215 594 276 654 8.22e-39 152
MS.gene058224.t1 AT2G29060 29.114 395 249 10 215 594 313 691 1.15e-38 152
MS.gene058224.t1 AT5G59450 30.077 389 249 12 218 595 222 598 3.88e-38 149
MS.gene058224.t1 AT1G55580 27.711 415 251 10 220 595 41 445 9.00e-38 145
MS.gene058224.t1 AT3G46600 29.765 383 242 11 224 595 5 371 1.17e-37 144
MS.gene058224.t1 AT3G46600 29.412 391 249 11 216 595 205 579 3.11e-37 146
MS.gene058224.t1 AT3G46600 29.412 391 249 11 216 595 173 547 6.37e-37 145
MS.gene058224.t1 AT1G63100 25.000 400 265 10 215 594 269 653 1.36e-35 142
MS.gene058224.t1 AT1G63100 25.000 400 265 10 215 594 244 628 1.51e-35 142
MS.gene058224.t1 AT2G29065 25.065 383 267 8 224 594 253 627 4.79e-35 140
MS.gene058224.t1 AT2G04890 25.729 377 258 7 224 595 54 413 9.87e-35 136
MS.gene058224.t1 AT1G07520 25.189 397 278 9 209 594 140 528 2.45e-34 137
MS.gene058224.t1 AT1G07520 25.189 397 278 9 209 594 140 528 2.45e-34 137
MS.gene058224.t1 AT1G07520 25.189 397 278 9 209 594 304 692 2.79e-34 139
MS.gene058224.t1 AT1G07520 25.189 397 278 9 209 594 305 693 2.99e-34 138
MS.gene058224.t1 AT5G66770 26.700 397 263 10 211 595 204 584 3.69e-34 137
MS.gene058224.t1 AT4G17230 25.648 386 262 9 220 595 155 525 1.19e-33 135
MS.gene058224.t1 AT4G17230 25.648 386 262 9 220 595 155 525 1.19e-33 135
MS.gene058224.t1 AT5G48150 23.958 384 272 9 220 595 119 490 3.02e-32 130
MS.gene058224.t1 AT5G48150 23.958 384 272 9 220 595 119 490 3.02e-32 130
MS.gene058224.t1 AT5G48150 23.958 384 272 9 220 595 119 490 3.02e-32 130
MS.gene058224.t1 AT5G48150 23.958 384 272 9 220 595 119 490 3.02e-32 130
MS.gene058224.t1 AT3G50650 26.462 359 231 10 251 595 203 542 2.57e-31 128
MS.gene058224.t1 AT2G37650 26.357 387 269 6 214 595 338 713 6.11e-31 129
MS.gene058224.t1 AT2G37650 26.357 387 269 6 214 595 326 701 6.27e-31 128
MS.gene058224.t1 AT1G50600 25.132 378 266 7 224 595 160 526 1.80e-30 125
MS.gene058224.t1 AT1G50600 25.132 378 266 7 224 595 231 597 3.62e-30 125
MS.gene058224.t1 AT1G50600 25.132 378 266 7 224 595 235 601 4.18e-30 125
MS.gene058224.t1 AT4G08250 26.034 411 262 12 204 594 88 476 3.30e-28 118
MS.gene058224.t1 AT1G21450 24.873 394 269 9 215 595 214 593 3.20e-23 104
MS.gene058224.t1 AT2G45160 25.414 362 242 10 241 595 299 639 1.80e-21 99.4
MS.gene058224.t1 AT1G50420 30.901 233 148 6 214 445 48 268 3.55e-21 97.4
MS.gene058224.t1 AT1G50420 31.410 156 95 4 446 595 330 479 1.95e-18 89.0
MS.gene058224.t1 AT3G60630 26.246 301 201 7 306 595 332 622 2.84e-20 95.5
MS.gene058224.t1 AT4G00150 26.357 387 241 13 218 595 203 554 3.67e-20 94.7
MS.gene058224.t1 AT4G37650 22.917 432 282 15 190 595 123 529 8.04e-19 90.5
MS.gene058224.t1 AT3G49950 24.359 390 263 12 224 594 31 407 1.35e-17 85.9
MS.gene058224.t1 AT5G52510 24.549 277 187 5 336 595 369 640 7.33e-16 81.6
MS.gene058224.t1 AT3G13840 25.887 282 183 10 221 490 145 412 3.60e-11 66.2

Find 76 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTTCTTTGCATCTTTA+AGG 0.061011 2.4:-56473966 None:intergenic
AATATCTCGACTGGTGTTAA+AGG 0.227086 2.4:-56473361 None:intergenic
CTGTTTCTTTGTCGATTCTT+TGG 0.234871 2.4:-56473789 None:intergenic
TTATGGTATTAGGAATATTT+TGG 0.239748 2.4:+56474518 MS.gene058224:CDS
TGTACTTCAGTTATGGTATT+AGG 0.241607 2.4:+56474508 MS.gene058224:CDS
TTCACTTGCTGAATCACTTT+TGG 0.248339 2.4:+56473636 MS.gene058224:CDS
CTTTGAAGTGGATGTGTTTC+CGG 0.249224 2.4:-56474100 None:intergenic
TTGATTCTTAATCACCCTTT+TGG 0.256538 2.4:+56473571 MS.gene058224:CDS
GAGTCTCTTTGCTCTAATTT+TGG 0.258364 2.4:+56473139 MS.gene058224:CDS
ATGGAAAACTTGTGGGAATT+TGG 0.283067 2.4:+56472983 MS.gene058224:CDS
CAGACAGAAACAGAGTTAAT+TGG 0.284565 2.4:-56474741 None:intergenic
ATAATTGTATTGAATGAAAA+AGG 0.286455 2.4:-56474180 None:intergenic
AGATCCTTGCTAACCTCTTC+AGG 0.292320 2.4:-56473859 None:intergenic
AAAGTTTCATCATCTTGATA+TGG 0.321809 2.4:-56473232 None:intergenic
TAATGAACCACACGCTTAAC+GGG 0.334947 2.4:-56473748 None:intergenic
TTGATTGAAGTGCTTGCATT+AGG 0.362361 2.4:-56474032 None:intergenic
GAATTTGGTGAGTTCAGTTT+TGG 0.366424 2.4:+56472998 MS.gene058224:CDS
ATAATGAACCACACGCTTAA+CGG 0.369400 2.4:-56473749 None:intergenic
ACTAAATCAAAATCATTAAC+AGG 0.380198 2.4:-56473445 None:intergenic
AACATTGAAACTTTAACAAA+TGG 0.394609 2.4:-56473916 None:intergenic
AATGGTCATTGTCTTCTTGT+TGG 0.397166 2.4:+56474705 MS.gene058224:CDS
TCTTGCACATTTGATATGAA+TGG 0.412387 2.4:+56474687 MS.gene058224:CDS
CAAAGAATCGACAAAGAAAC+AGG 0.418322 2.4:+56473790 MS.gene058224:CDS
AATGAGAGTAAGTTGTCAAC+TGG 0.424534 2.4:+56473481 MS.gene058224:CDS
GATTCTTTGGCAAAGTGCTT+CGG 0.434208 2.4:-56473776 None:intergenic
CAATTAACTCTGTTTCTGTC+TGG 0.434590 2.4:+56474742 MS.gene058224:CDS
ATGTTATCGATCTCGAAATT+CGG 0.443557 2.4:+56473989 MS.gene058224:CDS
GTCTATGTATCAAGCTGAAT+TGG 0.451983 2.4:+56474638 MS.gene058224:CDS
GAAGATCTAGGAGACTCTAA+TGG 0.460843 2.4:+56473112 MS.gene058224:CDS
AACTATAAATCCTATGGTAA+TGG 0.461785 2.4:+56474341 MS.gene058224:CDS
TAGAAGCTCGAGTGGACATT+CGG 0.469119 2.4:-56474058 None:intergenic
TGTCTTCTTGTTGGATGGAA+GGG 0.477569 2.4:+56474714 MS.gene058224:CDS
CATCATCTTGATATGGTTGG+TGG 0.478304 2.4:-56473225 None:intergenic
ATCTGTGATGGAAAACTTGT+GGG 0.486830 2.4:+56472976 None:intergenic
TTTCTCTTCATCAGAGAGAC+CGG 0.489642 2.4:-56473605 None:intergenic
AACTCTTTATCTTCGAAGAC+AGG 0.490618 2.4:+56473721 MS.gene058224:CDS
GAATCACTGTACTTCAGTTA+TGG 0.497629 2.4:+56474501 MS.gene058224:CDS
GGAGATATCATGAAGATAGC+TGG 0.498257 2.4:+56473502 MS.gene058224:CDS
TTTCATCATCTTGATATGGT+TGG 0.499177 2.4:-56473228 None:intergenic
TTGTCTTCTTGTTGGATGGA+AGG 0.499303 2.4:+56474713 MS.gene058224:CDS
TTTCATTGTATCAAGTCCCT+TGG 0.502952 2.4:-56473068 None:intergenic
CCGGAGAAAGAAAATCCAAA+AGG 0.504198 2.4:-56473586 None:intergenic
GTCATTGTCTTCTTGTTGGA+TGG 0.509549 2.4:+56474709 MS.gene058224:CDS
TTCAACTATAAGCTTTGAAG+TGG 0.517088 2.4:-56474112 None:intergenic
CAAAATCATTAACAGGATGA+AGG 0.518378 2.4:-56473438 None:intergenic
TGTTAGAACTATAAATCCTA+TGG 0.520922 2.4:+56474335 MS.gene058224:CDS
CTCTCATGACTGTTTCAAGT+TGG 0.524579 2.4:-56474311 None:intergenic
AGAAGAAGCTCTTCTTGTGA+TGG 0.532512 2.4:-56473178 None:intergenic
TCGATCTCGAAATTCGGAAA+GGG 0.539721 2.4:+56473995 MS.gene058224:CDS
ATCTTTATTTGATCCTGAAG+AGG 0.540063 2.4:+56473846 MS.gene058224:CDS
CGGAGAAAGAAAATCCAAAA+GGG 0.549231 2.4:-56473585 None:intergenic
AAATTCGGAAAGGGTTACAT+TGG 0.551296 2.4:+56474004 MS.gene058224:CDS
TCGACTGGTGTTAAAGGCGA+TGG 0.563201 2.4:-56473355 None:intergenic
TGTTTGGAAGATTGTATGAA+AGG 0.565315 2.4:+56474453 MS.gene058224:CDS
ATTGTGATTAGCTTCAGTCT+CGG 0.571904 2.4:-56474370 None:intergenic
TCTTCCTCAAAATCTATACC+AGG 0.583983 2.4:+56473547 MS.gene058224:CDS
AATCTGTGATGGAAAACTTG+TGG 0.585803 2.4:+56472975 None:intergenic
AGGTTATCACCTGATGATGA+AGG 0.586177 2.4:+56473409 MS.gene058224:CDS
ATCGATCTCGAAATTCGGAA+AGG 0.591892 2.4:+56473994 MS.gene058224:CDS
AAGATAACAGCTATTGCCAC+CGG 0.592969 2.4:+56474081 MS.gene058224:CDS
TGATCCTGAAGAGGTTAGCA+AGG 0.599740 2.4:+56473855 MS.gene058224:CDS
CTGGTGTTAAAGGCGATGGT+TGG 0.604359 2.4:-56473351 None:intergenic
GTTGCTAAAAGATTTCCTTG+TGG 0.611038 2.4:+56474660 MS.gene058224:CDS
TGATAACTTCAACATTGACA+TGG 0.613825 2.4:+56473255 MS.gene058224:CDS
GTTATCTTTAGAAGCTCGAG+TGG 0.620777 2.4:-56474066 None:intergenic
TCGGCTACAACCATTACCAT+AGG 0.626248 2.4:-56474351 None:intergenic
AGGTAACCCCGTTAAGCGTG+TGG 0.631363 2.4:+56473741 MS.gene058224:CDS
AAATTGAGAGTAAACTGCAA+CGG 0.631573 2.4:-56474259 None:intergenic
TCATGAAGATAGCTGGAACA+AGG 0.635820 2.4:+56473509 MS.gene058224:CDS
TGAAGTGGATGTGTTTCCGG+TGG 0.657076 2.4:-56474097 None:intergenic
CGAATGTGAAGATTGATGTG+TGG 0.657727 2.4:+56474565 MS.gene058224:CDS
GGGTGATTAAGAATCAAACC+TGG 0.658686 2.4:-56473565 None:intergenic
AAGCTTATAGTTGAAGACAC+CGG 0.666214 2.4:+56474120 MS.gene058224:CDS
ATTATGGCAAAGGATTCAAG+AGG 0.672484 2.4:+56473389 MS.gene058224:CDS
AATGAACCACACGCTTAACG+GGG 0.713768 2.4:-56473747 None:intergenic
AATGTGCAAGAATTTCCACA+AGG 0.746520 2.4:-56474675 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTGTATTGAATGAAAA+AGG - chr2.4:56474183-56474202 None:intergenic 15.0%
!! AACATTGAAACTTTAACAAA+TGG - chr2.4:56473919-56473938 None:intergenic 20.0%
!! ACTAAATCAAAATCATTAAC+AGG - chr2.4:56473448-56473467 None:intergenic 20.0%
!! ATATTGAAAAATTATGGCAA+AGG + chr2.4:56473379-56473398 MS.gene058224:CDS 20.0%
!!! TTATGGTATTAGGAATATTT+TGG + chr2.4:56474518-56474537 MS.gene058224:CDS 20.0%
! AAAGTTTCATCATCTTGATA+TGG - chr2.4:56473235-56473254 None:intergenic 25.0%
! AACTATAAATCCTATGGTAA+TGG + chr2.4:56474341-56474360 MS.gene058224:CDS 25.0%
! CTCTTCAAAAAATGCAATAA+GGG - chr2.4:56473893-56473912 None:intergenic 25.0%
! GTCGAGATATTGAAAAATTA+TGG + chr2.4:56473373-56473392 MS.gene058224:CDS 25.0%
! TGTTAGAACTATAAATCCTA+TGG + chr2.4:56474335-56474354 MS.gene058224:CDS 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.4:56474593-56474612 MS.gene058224:CDS 25.0%
!!! TAATTTTTCAATATCTCGAC+TGG - chr2.4:56473373-56473392 None:intergenic 25.0%
!!! TTCATCCAATGATACTTTTT+GGG + chr2.4:56473042-56473061 MS.gene058224:CDS 25.0%
AAATTGAGAGTAAACTGCAA+CGG - chr2.4:56474262-56474281 None:intergenic 30.0%
ATGTTATCGATCTCGAAATT+CGG + chr2.4:56473989-56474008 MS.gene058224:CDS 30.0%
CAAAATCATTAACAGGATGA+AGG - chr2.4:56473441-56473460 None:intergenic 30.0%
GAATCTTCTTTGCATCTTTA+AGG - chr2.4:56473969-56473988 None:intergenic 30.0%
GCTCTTCAAAAAATGCAATA+AGG - chr2.4:56473894-56473913 None:intergenic 30.0%
TCAAAAAATGCAATAAGGGT+TGG - chr2.4:56473889-56473908 None:intergenic 30.0%
TCTTGCACATTTGATATGAA+TGG + chr2.4:56474687-56474706 MS.gene058224:CDS 30.0%
TGATAACTTCAACATTGACA+TGG + chr2.4:56473255-56473274 MS.gene058224:CDS 30.0%
TGTACTTCAGTTATGGTATT+AGG + chr2.4:56474508-56474527 MS.gene058224:CDS 30.0%
TGTTTGGAAGATTGTATGAA+AGG + chr2.4:56474453-56474472 MS.gene058224:CDS 30.0%
TTTCATCATCTTGATATGGT+TGG - chr2.4:56473231-56473250 None:intergenic 30.0%
! ATCTTTATTTGATCCTGAAG+AGG + chr2.4:56473846-56473865 MS.gene058224:CDS 30.0%
! GTTCATCCAATGATACTTTT+TGG + chr2.4:56473041-56473060 MS.gene058224:CDS 30.0%
! TCCATTTTGTGACACAAAAA+AGG + chr2.4:56473315-56473334 MS.gene058224:CDS 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.4:56473571-56473590 MS.gene058224:CDS 30.0%
! TTTTCTGTAGAAGAAGATCT+AGG + chr2.4:56473100-56473119 MS.gene058224:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.4:56474413-56474432 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.4:56474588-56474607 MS.gene058224:CDS 30.0%
!! TTCAACTATAAGCTTTGAAG+TGG - chr2.4:56474115-56474134 None:intergenic 30.0%
!!! AAAATCCTCCAATTTTTTGC+CGG - chr2.4:56474142-56474161 None:intergenic 30.0%
!!! ATTAGGAATATTTTGGCTGA+AGG + chr2.4:56474525-56474544 MS.gene058224:CDS 30.0%
!!! TCCTTTTTTGTGTCACAAAA+TGG - chr2.4:56473319-56473338 None:intergenic 30.0%
AAATTCGGAAAGGGTTACAT+TGG + chr2.4:56474004-56474023 MS.gene058224:CDS 35.0%
AACTCTTTATCTTCGAAGAC+AGG + chr2.4:56473721-56473740 MS.gene058224:CDS 35.0%
AAGCTTATAGTTGAAGACAC+CGG + chr2.4:56474120-56474139 MS.gene058224:CDS 35.0%
AATGAGAGTAAGTTGTCAAC+TGG + chr2.4:56473481-56473500 MS.gene058224:CDS 35.0%
AATGGTCATTGTCTTCTTGT+TGG + chr2.4:56474705-56474724 MS.gene058224:CDS 35.0%
AATGTGCAAGAATTTCCACA+AGG - chr2.4:56474678-56474697 None:intergenic 35.0%
ATAATGAACCACACGCTTAA+CGG - chr2.4:56473752-56473771 None:intergenic 35.0%
ATGGAAAACTTGTGGGAATT+TGG + chr2.4:56472983-56473002 MS.gene058224:CDS 35.0%
ATTATGGCAAAGGATTCAAG+AGG + chr2.4:56473389-56473408 MS.gene058224:CDS 35.0%
CAAAGAATCGACAAAGAAAC+AGG + chr2.4:56473790-56473809 MS.gene058224:CDS 35.0%
CAGACAGAAACAGAGTTAAT+TGG - chr2.4:56474744-56474763 None:intergenic 35.0%
CGGAGAAAGAAAATCCAAAA+GGG - chr2.4:56473588-56473607 None:intergenic 35.0%
GAATCACTGTACTTCAGTTA+TGG + chr2.4:56474501-56474520 MS.gene058224:CDS 35.0%
GTCTATGTATCAAGCTGAAT+TGG + chr2.4:56474638-56474657 MS.gene058224:CDS 35.0%
GTTGCTAAAAGATTTCCTTG+TGG + chr2.4:56474660-56474679 MS.gene058224:CDS 35.0%
TCTTCCTCAAAATCTATACC+AGG + chr2.4:56473547-56473566 MS.gene058224:CDS 35.0%
TTTCATTGTATCAAGTCCCT+TGG - chr2.4:56473071-56473090 None:intergenic 35.0%
! AATATCTCGACTGGTGTTAA+AGG - chr2.4:56473364-56473383 None:intergenic 35.0%
! AGGATTTTTCCTTCATCATC+AGG - chr2.4:56473421-56473440 None:intergenic 35.0%
! CAAACCTGGTATAGATTTTG+AGG - chr2.4:56473554-56473573 None:intergenic 35.0%
! CCTTTTGGATTTTCTTTCTC+CGG + chr2.4:56473586-56473605 MS.gene058224:CDS 35.0%
! GAGTCTCTTTGCTCTAATTT+TGG + chr2.4:56473139-56473158 MS.gene058224:CDS 35.0%
! GTGATACTTTTGTTGATCAG+TGG - chr2.4:56473206-56473225 None:intergenic 35.0%
! TTGATTGAAGTGCTTGCATT+AGG - chr2.4:56474035-56474054 None:intergenic 35.0%
!! ATTGTGATTAGCTTCAGTCT+CGG - chr2.4:56474373-56474392 None:intergenic 35.0%
!! CAATTAACTCTGTTTCTGTC+TGG + chr2.4:56474742-56474761 MS.gene058224:CDS 35.0%
!! CTGTTTCTTTGTCGATTCTT+TGG - chr2.4:56473792-56473811 None:intergenic 35.0%
!! GAATTTGGTGAGTTCAGTTT+TGG + chr2.4:56472998-56473017 MS.gene058224:CDS 35.0%
!! GGTATCCCAAAAAGTATCAT+TGG - chr2.4:56473050-56473069 None:intergenic 35.0%
!! TAGTGCGCTTTTTGATTGTT+TGG + chr2.4:56474437-56474456 MS.gene058224:CDS 35.0%
!! TCTTTTCGAGAAAGATTCTG+AGG + chr2.4:56474233-56474252 MS.gene058224:CDS 35.0%
!! TTCACTTGCTGAATCACTTT+TGG + chr2.4:56473636-56473655 MS.gene058224:CDS 35.0%
!!! GATACTTTTTGGGATACCAA+GGG + chr2.4:56473052-56473071 MS.gene058224:CDS 35.0%
!!! TGATACTTTTTGGGATACCA+AGG + chr2.4:56473051-56473070 MS.gene058224:CDS 35.0%
AAGATAACAGCTATTGCCAC+CGG + chr2.4:56474081-56474100 MS.gene058224:CDS 40.0%
AGAAGAAGCTCTTCTTGTGA+TGG - chr2.4:56473181-56473200 None:intergenic 40.0%
ATCGATCTCGAAATTCGGAA+AGG + chr2.4:56473994-56474013 MS.gene058224:CDS 40.0%
CATCATCTTGATATGGTTGG+TGG - chr2.4:56473228-56473247 None:intergenic 40.0%
CCGGAGAAAGAAAATCCAAA+AGG - chr2.4:56473589-56473608 None:intergenic 40.0%
CGAATGTGAAGATTGATGTG+TGG + chr2.4:56474565-56474584 MS.gene058224:CDS 40.0%
GAAGATCTAGGAGACTCTAA+TGG + chr2.4:56473112-56473131 MS.gene058224:CDS 40.0%
GGAGATATCATGAAGATAGC+TGG + chr2.4:56473502-56473521 MS.gene058224:CDS 40.0%
GGGTGATTAAGAATCAAACC+TGG - chr2.4:56473568-56473587 None:intergenic 40.0%
GTCATTGTCTTCTTGTTGGA+TGG + chr2.4:56474709-56474728 MS.gene058224:CDS 40.0%
TAATGAACCACACGCTTAAC+GGG - chr2.4:56473751-56473770 None:intergenic 40.0%
TCATGAAGATAGCTGGAACA+AGG + chr2.4:56473509-56473528 MS.gene058224:CDS 40.0%
TCGATCTCGAAATTCGGAAA+GGG + chr2.4:56473995-56474014 MS.gene058224:CDS 40.0%
TGAAGACACCGGCAAAAAAT+TGG + chr2.4:56474131-56474150 MS.gene058224:CDS 40.0%
TGTCTTCTTGTTGGATGGAA+GGG + chr2.4:56474714-56474733 MS.gene058224:CDS 40.0%
TTGTCTTCTTGTTGGATGGA+AGG + chr2.4:56474713-56474732 MS.gene058224:CDS 40.0%
TTTCTCTTCATCAGAGAGAC+CGG - chr2.4:56473608-56473627 None:intergenic 40.0%
! AGGTTATCACCTGATGATGA+AGG + chr2.4:56473409-56473428 MS.gene058224:CDS 40.0%
! CTCTCATGACTGTTTCAAGT+TGG - chr2.4:56474314-56474333 None:intergenic 40.0%
! GATTCTTTGGCAAAGTGCTT+CGG - chr2.4:56473779-56473798 None:intergenic 40.0%
! GTTATCTTTAGAAGCTCGAG+TGG - chr2.4:56474069-56474088 None:intergenic 40.0%
!! CTTTGAAGTGGATGTGTTTC+CGG - chr2.4:56474103-56474122 None:intergenic 40.0%
!! GTTCAGTTTTGGTGTAACTG+AGG + chr2.4:56473009-56473028 MS.gene058224:CDS 40.0%
!! TTGGCTTGTGCTGAAAAAGT+TGG + chr2.4:56473655-56473674 MS.gene058224:CDS 40.0%
AATGAACCACACGCTTAACG+GGG - chr2.4:56473750-56473769 None:intergenic 45.0%
AGACACCGGCAAAAAATTGG+AGG + chr2.4:56474134-56474153 MS.gene058224:CDS 45.0%
AGATCCTTGCTAACCTCTTC+AGG - chr2.4:56473862-56473881 None:intergenic 45.0%
TAGAAGCTCGAGTGGACATT+CGG - chr2.4:56474061-56474080 None:intergenic 45.0%
TCGGCTACAACCATTACCAT+AGG - chr2.4:56474354-56474373 None:intergenic 45.0%
TGATCCTGAAGAGGTTAGCA+AGG + chr2.4:56473855-56473874 MS.gene058224:CDS 45.0%
TGAAGTGGATGTGTTTCCGG+TGG - chr2.4:56474100-56474119 None:intergenic 50.0%
! CTGGTGTTAAAGGCGATGGT+TGG - chr2.4:56473354-56473373 None:intergenic 50.0%
! TCGACTGGTGTTAAAGGCGA+TGG - chr2.4:56473358-56473377 None:intergenic 50.0%
AGGTAACCCCGTTAAGCGTG+TGG + chr2.4:56473741-56473760 MS.gene058224:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 56472983 56474776 56472983 ID=MS.gene058224
chr2.4 mRNA 56472983 56474776 56472983 ID=MS.gene058224.t1;Parent=MS.gene058224
chr2.4 exon 56472983 56474776 56472983 ID=MS.gene058224.t1.exon1;Parent=MS.gene058224.t1
chr2.4 CDS 56472983 56474776 56472983 ID=cds.MS.gene058224.t1;Parent=MS.gene058224.t1
Gene Sequence

>MS.gene058224

ATGGAAAACTTGTGGGAATTTGGTGAGTTCAGTTTTGGTGTAACTGAGGATAAACTCAGTTCATCCAATGATACTTTTTGGGATACCAAGGGACTTGATACAATGAAAAATGTTCAATTTTCTGTAGAAGAAGATCTAGGAGACTCTAATGGAATTGAGTCTCTTTGCTCTAATTTTGGTCTCTTTCAAGATGATCCATCACAAGAAGAGCTTCTTCTTTCCACTGATCAACAAAAGTATCACCACCAACCATATCAAGATGATGAAACTTTTGATAACTTCAACATTGACATGGTTCACTTTGATGAACAACAATGTGCTACAAGAATTCTTCCATTTTGTGACACAAAAAAGGACAATCAATATTACCAACCATCGCCTTTAACACCAGTCGAGATATTGAAAAATTATGGCAAAGGATTCAAGAGGTTATCACCTGATGATGAAGGAAAAATCCTTCATCCTGTTAATGATTTTGATTTAGTTACAGATAATCAAAATGAGAGTAAGTTGTCAACTGGAGATATCATGAAGATAGCTGGAACAAGGTTCATACAATCATCATCTTCCTCAAAATCTATACCAGGTTTGATTCTTAATCACCCTTTTGGATTTTCTTTCTCCGGTCTCTCTGATGAAGAGAAAGAAGATGTTTCACTTGCTGAATCACTTTTGGCTTGTGCTGAAAAAGTTGGATATCAACAATTTGAACGCGCTAGAAAATTGTTATCACATATCAACTCTTTATCTTCGAAGACAGGTAACCCCGTTAAGCGTGTGGTTCATTATTTTGCCGAAGCACTTTGCCAAAGAATCGACAAAGAAACAGGAAGAGTTTCATCTAACAATACACAAAAGATAGAATCTTTATTTGATCCTGAAGAGGTTAGCAAGGATCTAAATCCAACCCTTATTGCATTTTTTGAAGAGCTTCCATTTGTTAAAGTTTCAATGTTCACATGTGTACAAGCTTTAATAGAAAACCTTAAAGATGCAAAGAAGATTCATGTTATCGATCTCGAAATTCGGAAAGGGTTACATTGGACAATCCTAATGCAAGCACTTCAATCAAGAACCGAATGTCCACTCGAGCTTCTAAAGATAACAGCTATTGCCACCGGAAACACATCCACTTCAAAGCTTATAGTTGAAGACACCGGCAAAAAATTGGAGGATTTTGCACAAAGTTTGAACATACCTTTTTCATTCAATACAATTATTGTATCTAACTTGTTACATCTAAGAGAAGATCTTTTCGAGAAAGATTCTGAGGAAACCGTTGCAGTTTACTCTCAATTTGCTCTTAGAAGCAGTATTCAACAATCAGACCAACTTGAAACAGTCATGAGAGTTGTTAGAACTATAAATCCTATGGTAATGGTTGTAGCCGAGACTGAAGCTAATCACAATTCTAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCGCTTTTTGATTGTTTGGAAGATTGTATGAAAGGTGATGAGAAGAACAGAATGATTATAGAATCACTGTACTTCAGTTATGGTATTAGGAATATTTTGGCTGAAGGAGTTGAAAGAAAGAGTACGAATGTGAAGATTGATGTGTGGAGAGCATTTTTCACAAGATTTGGAATGGTTGAAACAGAATTGAGTATGAAGTCTATGTATCAAGCTGAATTGGTTGCTAAAAGATTTCCTTGTGGAAATTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTGTTGGATGGAAGGGAACACCAATTAACTCTGTTTCTGTCTGGAAATTCCTTTGA
Protein sequence

>MS.gene058224.t1

MENLWEFGEFSFGVTEDKLSSSNDTFWDTKGLDTMKNVQFSVEEDLGDSNGIESLCSNFGLFQDDPSQEELLLSTDQQKYHHQPYQDDETFDNFNIDMVHFDEQQCATRILPFCDTKKDNQYYQPSPLTPVEILKNYGKGFKRLSPDDEGKILHPVNDFDLVTDNQNESKLSTGDIMKIAGTRFIQSSSSSKSIPGLILNHPFGFSFSGLSDEEKEDVSLAESLLACAEKVGYQQFERARKLLSHINSLSSKTGNPVKRVVHYFAEALCQRIDKETGRVSSNNTQKIESLFDPEEVSKDLNPTLIAFFEELPFVKVSMFTCVQALIENLKDAKKIHVIDLEIRKGLHWTILMQALQSRTECPLELLKITAIATGNTSTSKLIVEDTGKKLEDFAQSLNIPFSFNTIIVSNLLHLREDLFEKDSEETVAVYSQFALRSSIQQSDQLETVMRVVRTINPMVMVVAETEANHNSKSFVNRFIEALFYFSALFDCLEDCMKGDEKNRMIIESLYFSYGIRNILAEGVERKSTNVKIDVWRAFFTRFGMVETELSMKSMYQAELVAKRFPCGNSCTFDMNGHCLLVGWKGTPINSVSVWKFL