Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058393.t1 | XP_013459206.1 | 79.6 | 269 | 49 | 1 | 1 | 263 | 1 | 269 | 6.30E-99 | 370.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058393.t1 | Q9M202 | 26.0 | 269 | 149 | 10 | 8 | 254 | 6 | 246 | 1.4e-09 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058393.t1 | A0A072V3Z9 | 79.6 | 269 | 49 | 1 | 1 | 263 | 1 | 269 | 4.5e-99 | 370.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene058393.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058393.t1 | MTR_3g408380 | 91.078 | 269 | 18 | 1 | 1 | 263 | 1 | 269 | 0.0 | 505 |
| MS.gene058393.t1 | MTR_3g008310 | 84.000 | 275 | 32 | 1 | 1 | 263 | 1 | 275 | 1.02e-169 | 469 |
| MS.gene058393.t1 | MTR_3g408420 | 64.493 | 276 | 84 | 3 | 1 | 263 | 1 | 275 | 7.92e-114 | 328 |
| MS.gene058393.t1 | MTR_0160s0030 | 26.797 | 306 | 164 | 10 | 8 | 255 | 6 | 309 | 8.79e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058393.t1 | AT3G60580 | 26.766 | 269 | 147 | 10 | 8 | 254 | 6 | 246 | 1.59e-13 | 69.3 |
| MS.gene058393.t1 | AT1G02030 | 26.482 | 253 | 152 | 8 | 8 | 254 | 7 | 231 | 1.58e-12 | 66.2 |
Find 43 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTGGTGGGTTACTTCT+TGG | 0.249003 | 3.2:-2811314 | None:intergenic |
| CAGGTACTTGGTTGTTGATT+GGG | 0.275325 | 3.2:-2811223 | None:intergenic |
| TCAGGTACTTGGTTGTTGAT+TGG | 0.290946 | 3.2:-2811224 | None:intergenic |
| CTTCTTGGATTAACAATTGA+TGG | 0.303146 | 3.2:-2811299 | None:intergenic |
| GCCAACTCAGCTGAGTATTC+AGG | 0.304238 | 3.2:-2811242 | None:intergenic |
| TGCTTTGACATCTTTGAATC+TGG | 0.309104 | 3.2:+2811818 | MS.gene058393:CDS |
| TTCAAAGATTGAGGATTGTT+TGG | 0.332480 | 3.2:-2811329 | None:intergenic |
| CTTTGAATCTGGTCACGTAT+TGG | 0.390852 | 3.2:+2811829 | MS.gene058393:CDS |
| ACAATCAGCGTATAGCGATT+TGG | 0.398813 | 3.2:+2811529 | MS.gene058393:CDS |
| CAGCTGAGTATTCAGGTACT+TGG | 0.400249 | 3.2:-2811235 | None:intergenic |
| GAGTGTGTCTCATTTGTAAC+AGG | 0.418825 | 3.2:+2811126 | MS.gene058393:CDS |
| AGGTACTTGGTTGTTGATTG+GGG | 0.434003 | 3.2:-2811222 | None:intergenic |
| CAGAATCATAATAATTGTAT+AGG | 0.437191 | 3.2:-2811871 | None:intergenic |
| GTGTTATTCTTAAGAATGAA+TGG | 0.439493 | 3.2:+2811576 | MS.gene058393:CDS |
| AATTTCACTTTCAAAGATTG+AGG | 0.444853 | 3.2:-2811338 | None:intergenic |
| AAATGTACCCCGTCAAACTT+TGG | 0.446972 | 3.2:+2811362 | MS.gene058393:CDS |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3.2:-2811264 | None:intergenic |
| AACAATCTAGCAGGAGAGAT+TGG | 0.456742 | 3.2:+2811740 | MS.gene058393:CDS |
| GGATTGGTAGTTTGGCATAG+TGG | 0.460724 | 3.2:-2811190 | None:intergenic |
| TCTTATCCAACAAACCCAAC+TGG | 0.466226 | 3.2:+2811404 | MS.gene058393:CDS |
| TATTCCTACCAAAGTTTGAC+GGG | 0.471579 | 3.2:-2811370 | None:intergenic |
| AAGATTGAGGATTGTTTGGT+GGG | 0.486813 | 3.2:-2811325 | None:intergenic |
| TCAGCCATATGAAACTGTCT+TGG | 0.489788 | 3.2:-2811446 | None:intergenic |
| CTATTCCTACCAAAGTTTGA+CGG | 0.495952 | 3.2:-2811371 | None:intergenic |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3.2:-2811272 | None:intergenic |
| TGAATCTGGTCACGTATTGG+AGG | 0.509112 | 3.2:+2811832 | MS.gene058393:CDS |
| AAAGATTGAGGATTGTTTGG+TGG | 0.514150 | 3.2:-2811326 | None:intergenic |
| GATTGGTAGTTTGGCATAGT+GGG | 0.520357 | 3.2:-2811189 | None:intergenic |
| CAAACCAAGACAGTTTCATA+TGG | 0.521057 | 3.2:+2811442 | MS.gene058393:CDS |
| GCAGGAGAGATTGGTAGAAG+TGG | 0.524466 | 3.2:+2811749 | MS.gene058393:CDS |
| AGGAAAACTAACAATCTAGC+AGG | 0.531732 | 3.2:+2811731 | MS.gene058393:CDS |
| ACCTGAATACTCAGCTGAGT+TGG | 0.539000 | 3.2:+2811241 | MS.gene058393:CDS |
| CGTATTGGAGGAACACATGA+AGG | 0.564625 | 3.2:+2811844 | MS.gene058393:CDS |
| GGTACTTGGTTGTTGATTGG+GGG | 0.573211 | 3.2:-2811221 | None:intergenic |
| GAATGAATGGAAACAAATTG+AGG | 0.585752 | 3.2:+2811589 | MS.gene058393:CDS |
| TCCAATGGAAAAGCTTTGGG+AGG | 0.590411 | 3.2:+2811155 | MS.gene058393:CDS |
| TCGGACATGAAGATATCCAT+AGG | 0.592226 | 3.2:+2811711 | MS.gene058393:CDS |
| ATAGAAGCTGCTGAAACACT+AGG | 0.609115 | 3.2:+2811554 | MS.gene058393:CDS |
| GTACCCCGTCAAACTTTGGT+AGG | 0.631191 | 3.2:+2811366 | MS.gene058393:CDS |
| ACAAATATCACACTCAAACA+CGG | 0.640237 | 3.2:-2811657 | None:intergenic |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3.2:+2811101 | None:intergenic |
| AGAGAATATAGAATTTAACA+TGG | 0.705093 | 3.2:+2811478 | MS.gene058393:CDS |
| ATTCCTACCAAAGTTTGACG+GGG | 0.741422 | 3.2:-2811369 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAGAATATAGAATTTAACA+TGG | + | chr3.2:2811478-2811497 | MS.gene058393:CDS | 20.0% |
| !! | CAGAATCATAATAATTGTAT+AGG | - | chr3.2:2811874-2811893 | None:intergenic | 20.0% |
| ! | AAGAAAAAAGCAAGTGAAAA+TGG | + | chr3.2:2811629-2811648 | MS.gene058393:CDS | 25.0% |
| ! | AATTTCACTTTCAAAGATTG+AGG | - | chr3.2:2811341-2811360 | None:intergenic | 25.0% |
| !! | GTGTTATTCTTAAGAATGAA+TGG | + | chr3.2:2811576-2811595 | MS.gene058393:CDS | 25.0% |
| ACAAATATCACACTCAAACA+CGG | - | chr3.2:2811660-2811679 | None:intergenic | 30.0% | |
| CTTCTTGGATTAACAATTGA+TGG | - | chr3.2:2811302-2811321 | None:intergenic | 30.0% | |
| GAATGAATGGAAACAAATTG+AGG | + | chr3.2:2811589-2811608 | MS.gene058393:CDS | 30.0% | |
| TTCAAAGATTGAGGATTGTT+TGG | - | chr3.2:2811332-2811351 | None:intergenic | 30.0% | |
| ! | ATTTTCCAATGGAAAAGCTT+TGG | + | chr3.2:2811151-2811170 | MS.gene058393:CDS | 30.0% |
| ! | CAATCTAGCAAAGATCTTTT+CGG | + | chr3.2:2811692-2811711 | MS.gene058393:CDS | 30.0% |
| ! | TTTTCCAATGGAAAAGCTTT+GGG | + | chr3.2:2811152-2811171 | MS.gene058393:CDS | 30.0% |
| !! | GTTTTGATCTTTTTCCAGTT+GGG | - | chr3.2:2811421-2811440 | None:intergenic | 30.0% |
| !!! | CTAGATTGTTAGTTTTCCTA+TGG | - | chr3.2:2811730-2811749 | None:intergenic | 30.0% |
| AAAGATTGAGGATTGTTTGG+TGG | - | chr3.2:2811329-2811348 | None:intergenic | 35.0% | |
| AAGATTGAGGATTGTTTGGT+GGG | - | chr3.2:2811328-2811347 | None:intergenic | 35.0% | |
| AGGAAAACTAACAATCTAGC+AGG | + | chr3.2:2811731-2811750 | MS.gene058393:CDS | 35.0% | |
| CAAACCAAGACAGTTTCATA+TGG | + | chr3.2:2811442-2811461 | MS.gene058393:CDS | 35.0% | |
| CTATTCCTACCAAAGTTTGA+CGG | - | chr3.2:2811374-2811393 | None:intergenic | 35.0% | |
| TATTCCTACCAAAGTTTGAC+GGG | - | chr3.2:2811373-2811392 | None:intergenic | 35.0% | |
| TGCTTTGACATCTTTGAATC+TGG | + | chr3.2:2811818-2811837 | MS.gene058393:CDS | 35.0% | |
| !! | CGTTTTGATCTTTTTCCAGT+TGG | - | chr3.2:2811422-2811441 | None:intergenic | 35.0% |
| !! | TGTAACAGGTCATTTTCCAA+TGG | + | chr3.2:2811140-2811159 | MS.gene058393:CDS | 35.0% |
| !!! | TTTTGGATGGATTGGTAGTT+TGG | - | chr3.2:2811201-2811220 | None:intergenic | 35.0% |
| AAATGTACCCCGTCAAACTT+TGG | + | chr3.2:2811362-2811381 | MS.gene058393:CDS | 40.0% | |
| AACAATCTAGCAGGAGAGAT+TGG | + | chr3.2:2811740-2811759 | MS.gene058393:CDS | 40.0% | |
| ACAATCAGCGTATAGCGATT+TGG | + | chr3.2:2811529-2811548 | MS.gene058393:CDS | 40.0% | |
| ATAGAAGCTGCTGAAACACT+AGG | + | chr3.2:2811554-2811573 | MS.gene058393:CDS | 40.0% | |
| ATTCCTACCAAAGTTTGACG+GGG | - | chr3.2:2811372-2811391 | None:intergenic | 40.0% | |
| CTTTGAATCTGGTCACGTAT+TGG | + | chr3.2:2811829-2811848 | MS.gene058393:CDS | 40.0% | |
| GAGTGTGTCTCATTTGTAAC+AGG | + | chr3.2:2811126-2811145 | MS.gene058393:CDS | 40.0% | |
| GATGTAGAGATAGAGTGAGT+TGG | - | chr3.2:2811275-2811294 | None:intergenic | 40.0% | |
| TCAGCCATATGAAACTGTCT+TGG | - | chr3.2:2811449-2811468 | None:intergenic | 40.0% | |
| TCAGGTACTTGGTTGTTGAT+TGG | - | chr3.2:2811227-2811246 | None:intergenic | 40.0% | |
| TCGGACATGAAGATATCCAT+AGG | + | chr3.2:2811711-2811730 | MS.gene058393:CDS | 40.0% | |
| TCTTATCCAACAAACCCAAC+TGG | + | chr3.2:2811404-2811423 | MS.gene058393:CDS | 40.0% | |
| ! | AGGTACTTGGTTGTTGATTG+GGG | - | chr3.2:2811225-2811244 | None:intergenic | 40.0% |
| ! | CAGGTACTTGGTTGTTGATT+GGG | - | chr3.2:2811226-2811245 | None:intergenic | 40.0% |
| ! | CTTTTTCCAGTTGGGTTTGT+TGG | - | chr3.2:2811413-2811432 | None:intergenic | 40.0% |
| ! | GATAGAGTGAGTTGGATGTT+TGG | - | chr3.2:2811267-2811286 | None:intergenic | 40.0% |
| ! | GATTGGTAGTTTGGCATAGT+GGG | - | chr3.2:2811192-2811211 | None:intergenic | 40.0% |
| !! | TTGTTTGGTGGGTTACTTCT+TGG | - | chr3.2:2811317-2811336 | None:intergenic | 40.0% |
| ACCTGAATACTCAGCTGAGT+TGG | + | chr3.2:2811241-2811260 | MS.gene058393:CDS | 45.0% | |
| CAGCTGAGTATTCAGGTACT+TGG | - | chr3.2:2811238-2811257 | None:intergenic | 45.0% | |
| CGTATTGGAGGAACACATGA+AGG | + | chr3.2:2811844-2811863 | MS.gene058393:CDS | 45.0% | |
| TGAATCTGGTCACGTATTGG+AGG | + | chr3.2:2811832-2811851 | MS.gene058393:CDS | 45.0% | |
| ! | GGATTGGTAGTTTGGCATAG+TGG | - | chr3.2:2811193-2811212 | None:intergenic | 45.0% |
| ! | GGTACTTGGTTGTTGATTGG+GGG | - | chr3.2:2811224-2811243 | None:intergenic | 45.0% |
| ! | TCCTCCCAAAGCTTTTCCAT+TGG | - | chr3.2:2811159-2811178 | None:intergenic | 45.0% |
| !! | TCCAATGGAAAAGCTTTGGG+AGG | + | chr3.2:2811155-2811174 | MS.gene058393:CDS | 45.0% |
| !! | TGGTTGTTGATTGGGGGTTT+TGG | - | chr3.2:2811218-2811237 | None:intergenic | 45.0% |
| !!! | ATTGGGGGTTTTGGATGGAT+TGG | - | chr3.2:2811209-2811228 | None:intergenic | 45.0% |
| !!! | TGTTGATTGGGGGTTTTGGA+TGG | - | chr3.2:2811214-2811233 | None:intergenic | 45.0% |
| GCAGGAGAGATTGGTAGAAG+TGG | + | chr3.2:2811749-2811768 | MS.gene058393:CDS | 50.0% | |
| GCCAACTCAGCTGAGTATTC+AGG | - | chr3.2:2811245-2811264 | None:intergenic | 50.0% | |
| GTACCCCGTCAAACTTTGGT+AGG | + | chr3.2:2811366-2811385 | MS.gene058393:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 2811110 | 2811901 | 2811110 | ID=MS.gene058393 |
| chr3.2 | mRNA | 2811110 | 2811901 | 2811110 | ID=MS.gene058393.t1;Parent=MS.gene058393 |
| chr3.2 | exon | 2811110 | 2811901 | 2811110 | ID=MS.gene058393.t1.exon1;Parent=MS.gene058393.t1 |
| chr3.2 | CDS | 2811110 | 2811901 | 2811110 | ID=cds.MS.gene058393.t1;Parent=MS.gene058393.t1 |
| Gene Sequence |
| Protein sequence |
