Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058395.t1 | XP_013459208.1 | 85.5 | 275 | 35 | 2 | 1 | 270 | 1 | 275 | 1.40E-117 | 432.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058395.t1 | Q9M202 | 26.4 | 277 | 147 | 10 | 7 | 261 | 5 | 246 | 2.4e-09 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058395.t1 | A0A072UTD1 | 85.5 | 275 | 35 | 2 | 1 | 270 | 1 | 275 | 9.9e-118 | 432.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene058395.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058395.t1 | MTR_3g408420 | 88.727 | 275 | 26 | 2 | 1 | 270 | 1 | 275 | 7.20e-177 | 488 |
| MS.gene058395.t1 | MTR_3g008310 | 63.636 | 275 | 95 | 3 | 1 | 270 | 1 | 275 | 2.82e-114 | 330 |
| MS.gene058395.t1 | MTR_3g408380 | 61.511 | 278 | 90 | 5 | 1 | 270 | 1 | 269 | 6.01e-111 | 321 |
| MS.gene058395.t1 | MTR_0160s0030 | 25.231 | 325 | 172 | 11 | 4 | 268 | 2 | 315 | 2.85e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058395.t1 | AT1G02030 | 26.296 | 270 | 148 | 8 | 5 | 263 | 4 | 233 | 3.40e-13 | 68.2 |
| MS.gene058395.t1 | AT3G60580 | 26.087 | 276 | 149 | 10 | 7 | 261 | 5 | 246 | 9.74e-12 | 64.3 |
Find 27 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTCGTAGACAATTTAATT+TGG | 0.272723 | 3.2:+2832766 | MS.gene058395:CDS |
| AACAATCTATCTGAAGAAAT+TGG | 0.300908 | 3.2:+2833091 | MS.gene058395:CDS |
| TGAGAATACACAAGTTAAAT+TGG | 0.302912 | 3.2:+2832865 | MS.gene058395:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3.2:+2832967 | MS.gene058395:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3.2:+2832880 | MS.gene058395:CDS |
| TCAGGTGCTTGGTTGTTGAT+TGG | 0.373001 | 3.2:-2832554 | None:intergenic |
| TATCATACCCTTGTAAACTT+TGG | 0.403944 | 3.2:+2832686 | MS.gene058395:CDS |
| CAACTGAGTGTTCAGGTGCT+TGG | 0.424891 | 3.2:-2832565 | None:intergenic |
| TCTGAAGAAATTGGTATAAG+TGG | 0.426032 | 3.2:+2833100 | MS.gene058395:CDS |
| CTATTATTTGTGTGAATGAA+TGG | 0.435873 | 3.2:+2832927 | MS.gene058395:CDS |
| GTTGACTCAACTGAGTGTTC+AGG | 0.442669 | 3.2:-2832572 | None:intergenic |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3.2:-2832594 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.471910 | 3.2:+2833153 | MS.gene058395:CDS |
| TATTCTTACCAAAGTTTACA+AGG | 0.477139 | 3.2:-2832694 | None:intergenic |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3.2:-2832480 | None:intergenic |
| AAGATCGAAGATTATGCATA+GGG | 0.485747 | 3.2:-2832649 | None:intergenic |
| TTTACTTGAGGAACACAAAA+CGG | 0.493212 | 3.2:+2833195 | MS.gene058395:CDS |
| TACAGAAGCTGCTGAAACAA+TGG | 0.521106 | 3.2:+2832904 | MS.gene058395:CDS |
| TGAATCTGATCATTTACTTG+AGG | 0.544553 | 3.2:+2833183 | MS.gene058395:CDS |
| ATAATCTTCGATCTTTGAAG+AGG | 0.557615 | 3.2:+2832657 | MS.gene058395:CDS |
| AAAGATCGAAGATTATGCAT+AGG | 0.557994 | 3.2:-2832650 | None:intergenic |
| AGAAATTGGTATAAGTGGAA+AGG | 0.574832 | 3.2:+2833105 | MS.gene058395:CDS |
| AGAGAATACAATATTTAATG+TGG | 0.616169 | 3.2:+2832793 | MS.gene058395:CDS |
| TGTGGCTGAAGAAAATGAAG+AGG | 0.616323 | 3.2:+2832811 | MS.gene058395:CDS |
| ATTCTTACCAAAGTTTACAA+GGG | 0.625545 | 3.2:-2832693 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3.2:+2832485 | MS.gene058395:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3.2:+2832940 | MS.gene058395:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAGATTGTTATTTTTCTTA+TGG | - | chr3.2:2833081-2833100 | None:intergenic | 15.0% |
| !! | AGAGAATACAATATTTAATG+TGG | + | chr3.2:2832793-2832812 | MS.gene058395:CDS | 20.0% |
| ! | AACAATCTATCTGAAGAAAT+TGG | + | chr3.2:2833091-2833110 | MS.gene058395:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3.2:2832980-2832999 | MS.gene058395:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3.2:2832967-2832986 | MS.gene058395:CDS | 25.0% |
| ! | ATTCTTACCAAAGTTTACAA+GGG | - | chr3.2:2832696-2832715 | None:intergenic | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3.2:2832927-2832946 | MS.gene058395:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3.2:2832880-2832899 | MS.gene058395:CDS | 25.0% |
| ! | TATTCTTACCAAAGTTTACA+AGG | - | chr3.2:2832697-2832716 | None:intergenic | 25.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3.2:2832865-2832884 | MS.gene058395:CDS | 25.0% |
| AAAGATCGAAGATTATGCAT+AGG | - | chr3.2:2832653-2832672 | None:intergenic | 30.0% | |
| AAGATCGAAGATTATGCATA+GGG | - | chr3.2:2832652-2832671 | None:intergenic | 30.0% | |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3.2:2832597-2832616 | None:intergenic | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3.2:2833105-2833124 | MS.gene058395:CDS | 30.0% | |
| TATCATACCCTTGTAAACTT+TGG | + | chr3.2:2832686-2832705 | MS.gene058395:CDS | 30.0% | |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3.2:2833100-2833119 | MS.gene058395:CDS | 30.0% | |
| TGAATCTGATCATTTACTTG+AGG | + | chr3.2:2833183-2833202 | MS.gene058395:CDS | 30.0% | |
| TTTACTTGAGGAACACAAAA+CGG | + | chr3.2:2833195-2833214 | MS.gene058395:CDS | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3.2:2832657-2832676 | MS.gene058395:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3.2:2832471-2832490 | MS.gene058395:CDS | 30.0% |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3.2:2833230-2833249 | None:intergenic | 30.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3.2:2832470-2832489 | MS.gene058395:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3.2:2832544-2832563 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3.2:2832545-2832564 | None:intergenic | 30.0% |
| !!! | AGATTATGCATAGGGTTTTT+TGG | - | chr3.2:2832644-2832663 | None:intergenic | 30.0% |
| !!! | GTTTTTTGGATTGGAAATTG+AGG | - | chr3.2:2832630-2832649 | None:intergenic | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3.2:2832482-2832501 | MS.gene058395:CDS | 30.0% |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3.2:2832483-2832502 | None:intergenic | 35.0% | |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3.2:2832766-2832785 | MS.gene058395:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3.2:2832940-2832959 | MS.gene058395:CDS | 35.0% | |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3.2:2832737-2832756 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3.2:2832745-2832764 | None:intergenic | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3.2:2832511-2832530 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3.2:2832728-2832747 | MS.gene058395:CDS | 35.0% |
| !! | ATTGGAGATTTTAGAGGGAT+TGG | - | chr3.2:2832539-2832558 | None:intergenic | 35.0% |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3.2:2832481-2832500 | MS.gene058395:CDS | 35.0% |
| !!! | ATGCATAGGGTTTTTTGGAT+TGG | - | chr3.2:2832639-2832658 | None:intergenic | 35.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3.2:2832746-2832765 | MS.gene058395:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAA+TGG | + | chr3.2:2832904-2832923 | MS.gene058395:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3.2:2833153-2833172 | MS.gene058395:CDS | 40.0% | |
| TGTGGCTGAAGAAAATGAAG+AGG | + | chr3.2:2832811-2832830 | MS.gene058395:CDS | 40.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3.2:2832746-2832765 | None:intergenic | 40.0% |
| TCAGGTGCTTGGTTGTTGAT+TGG | - | chr3.2:2832557-2832576 | None:intergenic | 45.0% | |
| ! | GTTGACTCAACTGAGTGTTC+AGG | - | chr3.2:2832575-2832594 | None:intergenic | 45.0% |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3.2:2832485-2832504 | MS.gene058395:CDS | 45.0% |
| CAACTGAGTGTTCAGGTGCT+TGG | - | chr3.2:2832568-2832587 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 2832440 | 2833252 | 2832440 | ID=MS.gene058395 |
| chr3.2 | mRNA | 2832440 | 2833252 | 2832440 | ID=MS.gene058395.t1;Parent=MS.gene058395 |
| chr3.2 | exon | 2832440 | 2833252 | 2832440 | ID=MS.gene058395.t1.exon1;Parent=MS.gene058395.t1 |
| chr3.2 | CDS | 2832440 | 2833252 | 2832440 | ID=cds.MS.gene058395.t1;Parent=MS.gene058395.t1 |
| Gene Sequence |
| Protein sequence |
