Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058397.t1 | XP_013459208.1 | 86.2 | 275 | 34 | 1 | 1 | 271 | 1 | 275 | 2.80E-118 | 434.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058397.t1 | Q9M202 | 26.9 | 279 | 144 | 10 | 7 | 262 | 5 | 246 | 9.0e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058397.t1 | A0A072UTD1 | 86.2 | 275 | 34 | 1 | 1 | 271 | 1 | 275 | 2.0e-118 | 434.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene058397.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058397.t1 | MTR_3g408420 | 90.909 | 275 | 21 | 1 | 1 | 271 | 1 | 275 | 1.12e-177 | 490 |
| MS.gene058397.t1 | MTR_3g008310 | 62.909 | 275 | 98 | 2 | 1 | 271 | 1 | 275 | 2.93e-114 | 330 |
| MS.gene058397.t1 | MTR_3g408380 | 61.511 | 278 | 91 | 4 | 1 | 271 | 1 | 269 | 5.70e-112 | 323 |
| MS.gene058397.t1 | MTR_0160s0030 | 25.000 | 324 | 175 | 9 | 4 | 269 | 2 | 315 | 4.29e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058397.t1 | AT1G02030 | 26.199 | 271 | 148 | 8 | 5 | 264 | 4 | 233 | 2.29e-12 | 65.9 |
| MS.gene058397.t1 | AT3G60580 | 26.354 | 277 | 148 | 10 | 7 | 262 | 5 | 246 | 9.86e-12 | 64.3 |
Find 27 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTCGTAGACAATTTAATT+TGG | 0.272723 | 3.2:+2845901 | MS.gene058397:CDS |
| TGAGAGTACACAAGTTAAAT+TGG | 0.302912 | 3.2:+2846000 | MS.gene058397:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3.2:+2846102 | MS.gene058397:CDS |
| AACAATCTATCCGAAGAAAT+TGG | 0.317101 | 3.2:+2846226 | MS.gene058397:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3.2:+2846015 | MS.gene058397:CDS |
| CTATTATTTGTGTGAATGAA+TGG | 0.399412 | 3.2:+2846062 | MS.gene058397:CDS |
| TCACGTGCTTGGTTGTTGAT+TGG | 0.399497 | 3.2:-2845686 | None:intergenic |
| TATCATACCCTTGTAAACTT+TGG | 0.406116 | 3.2:+2845821 | MS.gene058397:CDS |
| TCCACTTATACCAATTTCTT+CGG | 0.421044 | 3.2:-2846236 | None:intergenic |
| TCCGAAGAAATTGGTATAAG+TGG | 0.435137 | 3.2:+2846235 | MS.gene058397:CDS |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3.2:-2845726 | None:intergenic |
| TACAGAAGCTGCTGAAACAT+TGG | 0.473334 | 3.2:+2846039 | MS.gene058397:CDS |
| TATTCTTACCAAAGTTTACA+AGG | 0.477139 | 3.2:-2845829 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.480968 | 3.2:+2846288 | MS.gene058397:CDS |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3.2:-2845612 | None:intergenic |
| AAGATCGAAGATTATGCATA+GGG | 0.485747 | 3.2:-2845784 | None:intergenic |
| TGTACTTGAGGAACACAAAA+CGG | 0.523616 | 3.2:+2846330 | MS.gene058397:CDS |
| CAACTGAGTGTTCACGTGCT+TGG | 0.533072 | 3.2:-2845697 | None:intergenic |
| TGAATCTGATCATGTACTTG+AGG | 0.537940 | 3.2:+2846318 | MS.gene058397:CDS |
| ATAATCTTCGATCTTTGAAG+AGG | 0.557615 | 3.2:+2845792 | MS.gene058397:CDS |
| AAAGATCGAAGATTATGCAT+AGG | 0.557994 | 3.2:-2845785 | None:intergenic |
| AGAAATTGGTATAAGTGGAA+AGG | 0.571130 | 3.2:+2846240 | MS.gene058397:CDS |
| AGAGAATACAATATTTAATG+TGG | 0.616169 | 3.2:+2845928 | MS.gene058397:CDS |
| TGTGGCTGAAGAAAATGAAG+AGG | 0.616323 | 3.2:+2845946 | MS.gene058397:CDS |
| ATTCTTACCAAAGTTTACAA+GGG | 0.625545 | 3.2:-2845828 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3.2:+2845617 | MS.gene058397:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3.2:+2846075 | MS.gene058397:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAGATTGTTATTTTTCTTA+TGG | - | chr3.2:2846216-2846235 | None:intergenic | 15.0% |
| !! | AGAGAATACAATATTTAATG+TGG | + | chr3.2:2845928-2845947 | MS.gene058397:CDS | 20.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3.2:2846115-2846134 | MS.gene058397:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3.2:2846102-2846121 | MS.gene058397:CDS | 25.0% |
| ! | ATTCTTACCAAAGTTTACAA+GGG | - | chr3.2:2845831-2845850 | None:intergenic | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3.2:2846062-2846081 | MS.gene058397:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3.2:2846015-2846034 | MS.gene058397:CDS | 25.0% |
| ! | TATTCTTACCAAAGTTTACA+AGG | - | chr3.2:2845832-2845851 | None:intergenic | 25.0% |
| AAAGATCGAAGATTATGCAT+AGG | - | chr3.2:2845788-2845807 | None:intergenic | 30.0% | |
| AACAATCTATCCGAAGAAAT+TGG | + | chr3.2:2846226-2846245 | MS.gene058397:CDS | 30.0% | |
| AAGATCGAAGATTATGCATA+GGG | - | chr3.2:2845787-2845806 | None:intergenic | 30.0% | |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3.2:2845729-2845748 | None:intergenic | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3.2:2846240-2846259 | MS.gene058397:CDS | 30.0% | |
| TATCATACCCTTGTAAACTT+TGG | + | chr3.2:2845821-2845840 | MS.gene058397:CDS | 30.0% | |
| TCCACTTATACCAATTTCTT+CGG | - | chr3.2:2846239-2846258 | None:intergenic | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3.2:2845792-2845811 | MS.gene058397:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3.2:2845603-2845622 | MS.gene058397:CDS | 30.0% |
| ! | TGAGAGTACACAAGTTAAAT+TGG | + | chr3.2:2846000-2846019 | MS.gene058397:CDS | 30.0% |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3.2:2846365-2846384 | None:intergenic | 30.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3.2:2845602-2845621 | MS.gene058397:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3.2:2845676-2845695 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3.2:2845677-2845696 | None:intergenic | 30.0% |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3.2:2845762-2845781 | None:intergenic | 30.0% |
| !!! | AGATTATGCATAGGGTTTTT+TGG | - | chr3.2:2845779-2845798 | None:intergenic | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3.2:2845614-2845633 | MS.gene058397:CDS | 30.0% |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3.2:2845615-2845634 | None:intergenic | 35.0% | |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3.2:2845901-2845920 | MS.gene058397:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3.2:2846075-2846094 | MS.gene058397:CDS | 35.0% | |
| TCCGAAGAAATTGGTATAAG+TGG | + | chr3.2:2846235-2846254 | MS.gene058397:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3.2:2846318-2846337 | MS.gene058397:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3.2:2846330-2846349 | MS.gene058397:CDS | 35.0% | |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3.2:2845872-2845891 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3.2:2845880-2845899 | None:intergenic | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3.2:2845643-2845662 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3.2:2845863-2845882 | MS.gene058397:CDS | 35.0% |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3.2:2845613-2845632 | MS.gene058397:CDS | 35.0% |
| !!! | ATGCATAGGGTTTTTTGGAT+TGG | - | chr3.2:2845774-2845793 | None:intergenic | 35.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3.2:2845881-2845900 | MS.gene058397:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAT+TGG | + | chr3.2:2846039-2846058 | MS.gene058397:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3.2:2846288-2846307 | MS.gene058397:CDS | 40.0% | |
| TGTGGCTGAAGAAAATGAAG+AGG | + | chr3.2:2845946-2845965 | MS.gene058397:CDS | 40.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3.2:2845881-2845900 | None:intergenic | 40.0% |
| TCACGTGCTTGGTTGTTGAT+TGG | - | chr3.2:2845689-2845708 | None:intergenic | 45.0% | |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3.2:2845617-2845636 | MS.gene058397:CDS | 45.0% |
| CAACTGAGTGTTCACGTGCT+TGG | - | chr3.2:2845700-2845719 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 2845572 | 2846387 | 2845572 | ID=MS.gene058397 |
| chr3.2 | mRNA | 2845572 | 2846387 | 2845572 | ID=MS.gene058397.t1;Parent=MS.gene058397 |
| chr3.2 | exon | 2845572 | 2846387 | 2845572 | ID=MS.gene058397.t1.exon1;Parent=MS.gene058397.t1 |
| chr3.2 | CDS | 2845572 | 2846387 | 2845572 | ID=cds.MS.gene058397.t1;Parent=MS.gene058397.t1 |
| Gene Sequence |
| Protein sequence |
