Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058415.t1 | GAU35521.1 | 64.6 | 195 | 65 | 2 | 1 | 194 | 1 | 192 | 7.10E-64 | 253.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058415.t1 | Q9SZL8 | 34.9 | 215 | 131 | 5 | 4 | 210 | 35 | 248 | 1.5e-22 | 107.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058415.t1 | A0A2Z6MVW6 | 64.6 | 195 | 65 | 2 | 1 | 194 | 1 | 192 | 5.1e-64 | 253.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene058415.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058415.t1 | MTR_7g446520 | 40.179 | 224 | 109 | 3 | 6 | 220 | 47 | 254 | 9.73e-47 | 157 |
| MS.gene058415.t1 | MTR_8g035530 | 77.632 | 76 | 16 | 1 | 28 | 102 | 1 | 76 | 8.08e-35 | 119 |
| MS.gene058415.t1 | MTR_7g028855 | 43.421 | 152 | 81 | 1 | 33 | 179 | 112 | 263 | 1.95e-34 | 127 |
| MS.gene058415.t1 | MTR_7g446480 | 50.926 | 108 | 53 | 0 | 113 | 220 | 140 | 247 | 4.60e-33 | 121 |
| MS.gene058415.t1 | MTR_7g446510 | 50.000 | 108 | 54 | 0 | 113 | 220 | 155 | 262 | 7.78e-31 | 115 |
| MS.gene058415.t1 | MTR_5g076070 | 30.723 | 166 | 110 | 2 | 38 | 198 | 50 | 215 | 1.24e-27 | 106 |
| MS.gene058415.t1 | MTR_3g080590 | 54.023 | 87 | 40 | 0 | 5 | 91 | 69 | 155 | 9.73e-26 | 98.2 |
| MS.gene058415.t1 | MTR_6g022080 | 29.825 | 171 | 113 | 2 | 34 | 198 | 78 | 247 | 4.28e-24 | 96.3 |
| MS.gene058415.t1 | MTR_1g070355 | 30.645 | 186 | 120 | 3 | 41 | 220 | 74 | 256 | 1.78e-23 | 98.2 |
| MS.gene058415.t1 | MTR_0402s0040 | 31.395 | 172 | 113 | 3 | 45 | 212 | 30 | 200 | 2.09e-20 | 89.4 |
| MS.gene058415.t1 | MTR_6g051425 | 26.984 | 189 | 129 | 3 | 40 | 220 | 16 | 203 | 1.60e-18 | 82.8 |
| MS.gene058415.t1 | MTR_4g035820 | 30.811 | 185 | 117 | 5 | 44 | 219 | 78 | 260 | 3.75e-15 | 73.9 |
| MS.gene058415.t1 | MTR_7g061980 | 34.862 | 109 | 65 | 2 | 44 | 148 | 13 | 119 | 4.75e-15 | 69.3 |
| MS.gene058415.t1 | MTR_1g008290 | 29.197 | 137 | 86 | 3 | 81 | 217 | 1 | 126 | 1.00e-14 | 68.9 |
| MS.gene058415.t1 | MTR_5g015480 | 33.077 | 130 | 80 | 3 | 45 | 167 | 60 | 189 | 4.35e-14 | 69.7 |
| MS.gene058415.t1 | MTR_4g067290 | 35.000 | 100 | 63 | 2 | 44 | 141 | 59 | 158 | 8.56e-14 | 68.6 |
| MS.gene058415.t1 | MTR_2g098920 | 35.514 | 107 | 67 | 2 | 39 | 143 | 78 | 184 | 9.41e-14 | 68.6 |
| MS.gene058415.t1 | MTR_4g067290 | 35.000 | 100 | 63 | 2 | 44 | 141 | 59 | 158 | 9.42e-14 | 68.2 |
| MS.gene058415.t1 | MTR_4g067290 | 35.000 | 100 | 63 | 2 | 44 | 141 | 79 | 178 | 1.12e-13 | 68.6 |
| MS.gene058415.t1 | MTR_4g067290 | 35.000 | 100 | 63 | 2 | 44 | 141 | 77 | 176 | 1.16e-13 | 68.2 |
| MS.gene058415.t1 | MTR_4g067290 | 35.000 | 100 | 63 | 2 | 44 | 141 | 79 | 178 | 1.20e-13 | 68.2 |
| MS.gene058415.t1 | MTR_4g067290 | 35.000 | 100 | 63 | 2 | 44 | 141 | 119 | 218 | 1.38e-13 | 68.6 |
| MS.gene058415.t1 | MTR_7g074790 | 28.571 | 161 | 91 | 3 | 44 | 200 | 13 | 153 | 1.46e-11 | 62.0 |
| MS.gene058415.t1 | MTR_7g113010 | 28.455 | 123 | 72 | 2 | 44 | 163 | 460 | 569 | 5.94e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058415.t1 | AT4G38180 | 34.884 | 215 | 131 | 5 | 4 | 210 | 35 | 248 | 6.94e-27 | 108 |
| MS.gene058415.t1 | AT3G07500 | 36.842 | 152 | 82 | 6 | 16 | 157 | 3 | 150 | 1.85e-17 | 78.2 |
| MS.gene058415.t1 | AT3G59470 | 35.514 | 107 | 67 | 2 | 39 | 143 | 80 | 186 | 6.95e-14 | 69.3 |
| MS.gene058415.t1 | AT3G59470 | 35.514 | 107 | 67 | 2 | 39 | 143 | 68 | 174 | 7.04e-14 | 68.9 |
| MS.gene058415.t1 | AT3G59470 | 35.514 | 107 | 67 | 2 | 39 | 143 | 68 | 174 | 7.04e-14 | 68.9 |
| MS.gene058415.t1 | AT5G28530 | 26.966 | 178 | 122 | 4 | 44 | 214 | 58 | 234 | 1.68e-13 | 69.3 |
| MS.gene058415.t1 | AT5G28530 | 26.966 | 178 | 122 | 4 | 44 | 214 | 58 | 234 | 1.88e-13 | 69.3 |
| MS.gene058415.t1 | AT5G28530 | 26.966 | 178 | 122 | 4 | 44 | 214 | 58 | 234 | 1.88e-13 | 69.3 |
| MS.gene058415.t1 | AT5G28530 | 26.966 | 178 | 122 | 4 | 44 | 214 | 58 | 234 | 2.39e-13 | 68.9 |
| MS.gene058415.t1 | AT5G28530 | 26.966 | 178 | 122 | 4 | 44 | 214 | 58 | 234 | 2.58e-13 | 68.9 |
| MS.gene058415.t1 | AT4G12850 | 37.391 | 115 | 67 | 3 | 39 | 152 | 9 | 119 | 3.23e-13 | 65.1 |
| MS.gene058415.t1 | AT4G12850 | 39.216 | 102 | 60 | 2 | 39 | 139 | 9 | 109 | 6.50e-13 | 63.9 |
| MS.gene058415.t1 | AT1G10240 | 28.916 | 166 | 109 | 5 | 44 | 200 | 51 | 216 | 1.56e-12 | 66.6 |
| MS.gene058415.t1 | AT1G10240 | 28.916 | 166 | 109 | 5 | 44 | 200 | 51 | 216 | 1.56e-12 | 66.6 |
| MS.gene058415.t1 | AT4G12850 | 36.066 | 122 | 73 | 3 | 39 | 156 | 9 | 129 | 2.05e-12 | 63.9 |
| MS.gene058415.t1 | AT5G18960 | 39.175 | 97 | 47 | 3 | 44 | 139 | 46 | 131 | 2.00e-11 | 63.2 |
| MS.gene058415.t1 | AT5G18960 | 39.175 | 97 | 47 | 3 | 44 | 139 | 46 | 131 | 2.00e-11 | 63.2 |
Find 50 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAATAAGTGGAGAGTTTC+TGG | 0.119642 | 3.2:-3078562 | MS.gene058415:CDS |
| TGTTAGAACCTGTCATATTT+TGG | 0.145212 | 3.2:-3078422 | MS.gene058415:CDS |
| TCACTCAATTGACGGTTCTT+AGG | 0.285255 | 3.2:+3078484 | None:intergenic |
| TGCTGCGCTGAGTTATTTGT+AGG | 0.328753 | 3.2:-3078287 | None:intergenic |
| TGAAGAAGGAAAAGAGGATA+TGG | 0.353695 | 3.2:-3078866 | MS.gene058415:CDS |
| TGGATACTTACAAGAAGATT+AGG | 0.354642 | 3.2:-3078846 | MS.gene058415:CDS |
| TCCAACCCGTTATGCTCATT+TGG | 0.356553 | 3.2:-3078509 | MS.gene058415:CDS |
| AAAAGGAGGTCATGAGAGTC+TGG | 0.365097 | 3.2:-3078383 | MS.gene058415:CDS |
| AGACATAAATGGTGAAGATT+TGG | 0.373826 | 3.2:-3078911 | MS.gene058415:CDS |
| GCAAACGATGGAGACATAAA+TGG | 0.387588 | 3.2:-3078922 | MS.gene058415:CDS |
| TCTGATAAAGCCCTTGTTGA+TGG | 0.437123 | 3.2:-3078460 | MS.gene058415:CDS |
| TATCAGATTCACTCAATTGA+CGG | 0.439464 | 3.2:+3078476 | None:intergenic |
| GAAGGAAAGGCAAGAATAGA+AGG | 0.447532 | 3.2:-3078322 | MS.gene058415:CDS |
| GGATACTTACAAGAAGATTA+GGG | 0.465987 | 3.2:-3078845 | MS.gene058415:CDS |
| CAAGAAGATTAGGGAATTGA+CGG | 0.467663 | 3.2:-3078836 | MS.gene058415:CDS |
| AGGAACCAAATGAGCATAAC+GGG | 0.471013 | 3.2:+3078504 | None:intergenic |
| ATTTATGTCTCCATCGTTTG+CGG | 0.473976 | 3.2:+3078924 | None:intergenic |
| AATGGTGTGTTTAATGAAGA+AGG | 0.477078 | 3.2:-3078880 | MS.gene058415:CDS |
| TAAATGTTGATACTAATAAG+TGG | 0.482914 | 3.2:-3078573 | MS.gene058415:CDS |
| GAAGATTTGGCCAGCGGTAA+TGG | 0.496494 | 3.2:-3078898 | MS.gene058415:CDS |
| GGATGATGATATAAGAGGAT+TGG | 0.496802 | 3.2:-3078815 | MS.gene058415:CDS |
| TTGACGGATGATGATATAAG+AGG | 0.497315 | 3.2:-3078820 | MS.gene058415:CDS |
| GAAATCTATGCAGATTGTCA+TGG | 0.498853 | 3.2:-3078757 | MS.gene058415:CDS |
| TATAACTACTTATACAATGA+AGG | 0.501991 | 3.2:-3078340 | MS.gene058415:CDS |
| AGGATTGGAATTTGATTCAG+AGG | 0.508865 | 3.2:-3078800 | MS.gene058415:CDS |
| GATGTAAACAAAGATGATGA+TGG | 0.508994 | 3.2:-3078715 | MS.gene058415:CDS |
| AGAAACAAAGTACATATTCG+AGG | 0.529955 | 3.2:-3078639 | MS.gene058415:CDS |
| TGCAGATTGTCATGGGTTTG+TGG | 0.531824 | 3.2:-3078749 | MS.gene058415:CDS |
| AAATCTATGCAGATTGTCAT+GGG | 0.533515 | 3.2:-3078756 | MS.gene058415:CDS |
| ATTTAAATGGCCGCAAACGA+TGG | 0.539363 | 3.2:-3078934 | None:intergenic |
| TGCAGAACACTTAGTTCGAA+TGG | 0.539821 | 3.2:+3078612 | None:intergenic |
| GGAAAGGCAAGAATAGAAGG+TGG | 0.544624 | 3.2:-3078319 | MS.gene058415:CDS |
| TAGGAACCAAATGAGCATAA+CGG | 0.549503 | 3.2:+3078503 | None:intergenic |
| CATAAAACCCAAAATATGAC+AGG | 0.552239 | 3.2:+3078414 | None:intergenic |
| ATTAAACACACCATTACCGC+TGG | 0.563951 | 3.2:+3078888 | None:intergenic |
| GTTTAATGAAGAAGGAAAAG+AGG | 0.567512 | 3.2:-3078872 | MS.gene058415:CDS |
| AACGGGTTGGAGTTAACTCA+TGG | 0.579913 | 3.2:+3078521 | None:intergenic |
| CTACTTATACAATGAAGGAA+AGG | 0.581665 | 3.2:-3078335 | MS.gene058415:CDS |
| ATGTAAACAAAGATGATGAT+GGG | 0.584402 | 3.2:-3078714 | MS.gene058415:CDS |
| AGTTAACTCATGGTTGTGTG+AGG | 0.591294 | 3.2:+3078531 | None:intergenic |
| ACCAAATGAGCATAACGGGT+TGG | 0.596668 | 3.2:+3078508 | None:intergenic |
| TCGATTAGTATGTAATAGAG+AGG | 0.600970 | 3.2:-3078677 | MS.gene058415:CDS |
| AAAGGAGGTCATGAGAGTCT+GGG | 0.612373 | 3.2:-3078382 | MS.gene058415:CDS |
| GTGCATGAAGACCATCAACA+AGG | 0.624046 | 3.2:+3078449 | None:intergenic |
| GTTAACTCATGGTTGTGTGA+GGG | 0.630795 | 3.2:+3078532 | None:intergenic |
| CGATTAGTATGTAATAGAGA+GGG | 0.642038 | 3.2:-3078676 | MS.gene058415:CDS |
| TTCGAACTAAGTGTTCTGCA+AGG | 0.661356 | 3.2:-3078609 | MS.gene058415:CDS |
| TGCATGAAGACCATCAACAA+GGG | 0.667132 | 3.2:+3078450 | None:intergenic |
| GTTGATGGTCTTCATGCACA+CGG | 0.677402 | 3.2:-3078445 | MS.gene058415:CDS |
| AATGGTGAAGATTTGGCCAG+CGG | 0.682068 | 3.2:-3078904 | MS.gene058415:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATGTTGATACTAATAAG+TGG | - | chr3.2:3078643-3078662 | MS.gene058415:CDS | 20.0% |
| !! | TATAACTACTTATACAATGA+AGG | - | chr3.2:3078876-3078895 | MS.gene058415:CDS | 20.0% |
| ! | ATGTAAACAAAGATGATGAT+GGG | - | chr3.2:3078502-3078521 | MS.gene058415:CDS | 25.0% |
| !!! | TCATATTTTGGGTTTTATGA+TGG | - | chr3.2:3078806-3078825 | MS.gene058415:CDS | 25.0% |
| AAATCTATGCAGATTGTCAT+GGG | - | chr3.2:3078460-3078479 | MS.gene058415:CDS | 30.0% | |
| AATGGTGTGTTTAATGAAGA+AGG | - | chr3.2:3078336-3078355 | MS.gene058415:CDS | 30.0% | |
| AGAAACAAAGTACATATTCG+AGG | - | chr3.2:3078577-3078596 | MS.gene058415:CDS | 30.0% | |
| AGACATAAATGGTGAAGATT+TGG | - | chr3.2:3078305-3078324 | MS.gene058415:CDS | 30.0% | |
| CATAAAACCCAAAATATGAC+AGG | + | chr3.2:3078805-3078824 | None:intergenic | 30.0% | |
| CGATTAGTATGTAATAGAGA+GGG | - | chr3.2:3078540-3078559 | MS.gene058415:CDS | 30.0% | |
| CTACTTATACAATGAAGGAA+AGG | - | chr3.2:3078881-3078900 | MS.gene058415:CDS | 30.0% | |
| GATGTAAACAAAGATGATGA+TGG | - | chr3.2:3078501-3078520 | MS.gene058415:CDS | 30.0% | |
| GGATACTTACAAGAAGATTA+GGG | - | chr3.2:3078371-3078390 | MS.gene058415:CDS | 30.0% | |
| GTTTAATGAAGAAGGAAAAG+AGG | - | chr3.2:3078344-3078363 | MS.gene058415:CDS | 30.0% | |
| TATCAGATTCACTCAATTGA+CGG | + | chr3.2:3078743-3078762 | None:intergenic | 30.0% | |
| TCGATTAGTATGTAATAGAG+AGG | - | chr3.2:3078539-3078558 | MS.gene058415:CDS | 30.0% | |
| TGGATACTTACAAGAAGATT+AGG | - | chr3.2:3078370-3078389 | MS.gene058415:CDS | 30.0% | |
| ! | GTTAGAACCTGTCATATTTT+GGG | - | chr3.2:3078795-3078814 | MS.gene058415:CDS | 30.0% |
| ! | TGTTAGAACCTGTCATATTT+TGG | - | chr3.2:3078794-3078813 | MS.gene058415:CDS | 30.0% |
| ACTAATAAGTGGAGAGTTTC+TGG | - | chr3.2:3078654-3078673 | MS.gene058415:CDS | 35.0% | |
| AGGATTGGAATTTGATTCAG+AGG | - | chr3.2:3078416-3078435 | MS.gene058415:CDS | 35.0% | |
| ATTTATGTCTCCATCGTTTG+CGG | + | chr3.2:3078295-3078314 | None:intergenic | 35.0% | |
| CAAGAAGATTAGGGAATTGA+CGG | - | chr3.2:3078380-3078399 | MS.gene058415:CDS | 35.0% | |
| GAAATCTATGCAGATTGTCA+TGG | - | chr3.2:3078459-3078478 | MS.gene058415:CDS | 35.0% | |
| GGATGATGATATAAGAGGAT+TGG | - | chr3.2:3078401-3078420 | MS.gene058415:CDS | 35.0% | |
| TAGGAACCAAATGAGCATAA+CGG | + | chr3.2:3078716-3078735 | None:intergenic | 35.0% | |
| TGAAGAAGGAAAAGAGGATA+TGG | - | chr3.2:3078350-3078369 | MS.gene058415:CDS | 35.0% | |
| TTTATGATGGCTCAAAAAGG+AGG | - | chr3.2:3078819-3078838 | MS.gene058415:CDS | 35.0% | |
| ! | TTGACGGATGATGATATAAG+AGG | - | chr3.2:3078396-3078415 | MS.gene058415:CDS | 35.0% |
| !! | GGTTTTATGATGGCTCAAAA+AGG | - | chr3.2:3078816-3078835 | MS.gene058415:CDS | 35.0% |
| AGGAACCAAATGAGCATAAC+GGG | + | chr3.2:3078715-3078734 | None:intergenic | 40.0% | |
| AGTTAACTCATGGTTGTGTG+AGG | + | chr3.2:3078688-3078707 | None:intergenic | 40.0% | |
| ATTAAACACACCATTACCGC+TGG | + | chr3.2:3078331-3078350 | None:intergenic | 40.0% | |
| GAAGGAAAGGCAAGAATAGA+AGG | - | chr3.2:3078894-3078913 | MS.gene058415:CDS | 40.0% | |
| GCAAACGATGGAGACATAAA+TGG | - | chr3.2:3078294-3078313 | MS.gene058415:CDS | 40.0% | |
| GTTAACTCATGGTTGTGTGA+GGG | + | chr3.2:3078687-3078706 | None:intergenic | 40.0% | |
| TCACTCAATTGACGGTTCTT+AGG | + | chr3.2:3078735-3078754 | None:intergenic | 40.0% | |
| TCTGATAAAGCCCTTGTTGA+TGG | - | chr3.2:3078756-3078775 | MS.gene058415:CDS | 40.0% | |
| TGCAGAACACTTAGTTCGAA+TGG | + | chr3.2:3078607-3078626 | None:intergenic | 40.0% | |
| TGCATGAAGACCATCAACAA+GGG | + | chr3.2:3078769-3078788 | None:intergenic | 40.0% | |
| TTCGAACTAAGTGTTCTGCA+AGG | - | chr3.2:3078607-3078626 | MS.gene058415:CDS | 40.0% | |
| AAAAGGAGGTCATGAGAGTC+TGG | - | chr3.2:3078833-3078852 | MS.gene058415:CDS | 45.0% | |
| AAAGGAGGTCATGAGAGTCT+GGG | - | chr3.2:3078834-3078853 | MS.gene058415:CDS | 45.0% | |
| AATGGTGAAGATTTGGCCAG+CGG | - | chr3.2:3078312-3078331 | MS.gene058415:CDS | 45.0% | |
| GGAAAGGCAAGAATAGAAGG+TGG | - | chr3.2:3078897-3078916 | MS.gene058415:CDS | 45.0% | |
| GTGCATGAAGACCATCAACA+AGG | + | chr3.2:3078770-3078789 | None:intergenic | 45.0% | |
| TCCAACCCGTTATGCTCATT+TGG | - | chr3.2:3078707-3078726 | MS.gene058415:CDS | 45.0% | |
| TGCAGATTGTCATGGGTTTG+TGG | - | chr3.2:3078467-3078486 | MS.gene058415:CDS | 45.0% | |
| !! | AACGGGTTGGAGTTAACTCA+TGG | + | chr3.2:3078698-3078717 | None:intergenic | 45.0% |
| !! | ACCAAATGAGCATAACGGGT+TGG | + | chr3.2:3078711-3078730 | None:intergenic | 45.0% |
| !! | ATGAGAGTCTGGGATTTTGC+AGG | - | chr3.2:3078844-3078863 | MS.gene058415:CDS | 45.0% |
| !! | GTTGATGGTCTTCATGCACA+CGG | - | chr3.2:3078771-3078790 | MS.gene058415:CDS | 45.0% |
| GAAGATTTGGCCAGCGGTAA+TGG | - | chr3.2:3078318-3078337 | MS.gene058415:CDS | 50.0% | |
| !! | GAGTCTGGGATTTTGCAGGA+AGG | - | chr3.2:3078848-3078867 | MS.gene058415:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 3078288 | 3078950 | 3078288 | ID=MS.gene058415 |
| chr3.2 | mRNA | 3078288 | 3078950 | 3078288 | ID=MS.gene058415.t1;Parent=MS.gene058415 |
| chr3.2 | exon | 3078288 | 3078950 | 3078288 | ID=MS.gene058415.t1.exon1;Parent=MS.gene058415.t1 |
| chr3.2 | CDS | 3078288 | 3078950 | 3078288 | ID=cds.MS.gene058415.t1;Parent=MS.gene058415.t1 |
| Gene Sequence |
| Protein sequence |
