AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058766


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058766.t1 MTR_3g005480 84.937 239 26 1 22 260 36 264 1.25e-150 421
MS.gene058766.t1 MTR_3g005490 78.008 241 42 2 22 262 36 265 2.74e-134 380
MS.gene058766.t1 MTR_5g070440 70.539 241 69 1 22 262 36 274 1.72e-122 350
MS.gene058766.t1 MTR_3g005420 65.560 241 81 1 22 262 304 542 1.14e-107 322
MS.gene058766.t1 MTR_3g005420 55.556 234 78 4 22 253 55 264 1.15e-72 231
MS.gene058766.t1 MTR_3g005460 63.563 247 84 2 22 262 277 523 1.60e-103 310
MS.gene058766.t1 MTR_3g005460 55.459 229 77 3 22 250 40 243 4.94e-75 237
MS.gene058766.t1 MTR_3g005420 54.357 241 73 2 22 262 285 488 5.87e-80 249
MS.gene058766.t1 MTR_3g005420 55.556 234 78 4 22 253 36 245 1.70e-73 232
MS.gene058766.t1 MTR_3g005440 62.694 193 65 1 22 214 257 442 2.07e-75 236
MS.gene058766.t1 MTR_3g005440 50.000 238 66 3 18 253 31 217 5.85e-63 204
MS.gene058766.t1 MTR_3g005495 51.852 243 84 4 18 259 24 234 3.90e-73 231
MS.gene058766.t1 MTR_3g005495 52.766 235 90 6 22 254 258 473 1.57e-69 222
MS.gene058766.t1 MTR_1g015350 39.791 191 40 5 50 239 58 174 3.59e-29 108
MS.gene058766.t1 MTR_7g050710 30.526 285 149 10 22 259 28 310 3.70e-28 110
MS.gene058766.t1 MTR_3g098790 29.749 279 149 11 23 259 29 302 4.48e-26 104
MS.gene058766.t1 MTR_3g061310 30.597 268 153 8 19 255 20 285 5.45e-26 103
MS.gene058766.t1 MTR_3g061320 31.884 276 133 11 19 255 20 279 3.28e-25 101
MS.gene058766.t1 MTR_3g061370 29.104 268 148 6 22 255 22 281 1.57e-24 99.4
MS.gene058766.t1 MTR_0152s0100 42.982 114 62 1 147 260 2 112 1.61e-24 95.1
MS.gene058766.t1 MTR_0152s0100 42.982 114 62 1 147 260 2 112 1.61e-24 95.1
MS.gene058766.t1 MTR_3g061350 29.927 274 146 9 19 255 20 284 5.96e-24 97.8
MS.gene058766.t1 MTR_3g061340 32.061 262 146 10 19 255 20 274 8.54e-24 97.4
MS.gene058766.t1 MTR_7g050560 26.007 273 158 5 23 259 29 293 9.25e-24 98.2
MS.gene058766.t1 MTR_1g034240 48.810 84 43 0 22 105 22 105 1.12e-23 99.4
MS.gene058766.t1 MTR_1g034240 36.364 132 71 4 133 257 268 393 5.19e-16 77.4
MS.gene058766.t1 MTR_1g108780 26.804 291 151 8 20 257 10 291 2.02e-23 99.0
MS.gene058766.t1 MTR_8g103940 26.316 304 156 9 8 257 43 332 1.38e-22 96.7
MS.gene058766.t1 MTR_7g050590 24.828 290 157 6 23 259 2 283 1.77e-22 94.4
MS.gene058766.t1 MTR_7g050580 27.612 268 160 8 23 259 29 293 1.57e-21 92.0
MS.gene058766.t1 MTR_5g044670 24.460 278 166 6 23 259 32 306 2.94e-21 91.3
MS.gene058766.t1 MTR_1g035460 26.351 296 148 8 23 257 2 288 1.85e-20 90.1
MS.gene058766.t1 MTR_4g068320 38.835 103 62 1 4 105 2 104 1.59e-19 87.4
MS.gene058766.t1 MTR_4g068320 36.567 134 72 4 133 259 264 391 1.09e-16 79.3
MS.gene058766.t1 MTR_1g034210 49.333 75 38 0 22 96 22 96 1.11e-18 84.3
MS.gene058766.t1 MTR_1g034210 49.333 75 38 0 22 96 22 96 2.07e-18 84.0
MS.gene058766.t1 MTR_1g034210 33.871 124 69 4 141 257 242 359 7.57e-12 65.1
MS.gene058766.t1 MTR_3g061330 29.825 171 91 3 22 165 25 193 1.22e-17 80.1
MS.gene058766.t1 MTR_3g061290 41.509 106 59 2 19 122 20 124 1.63e-17 79.0
MS.gene058766.t1 MTR_7g050720 34.737 95 62 0 23 117 26 120 4.96e-15 73.9
MS.gene058766.t1 MTR_7g050640 34.375 96 63 0 12 107 18 113 6.83e-14 67.0
MS.gene058766.t1 MTR_3g061220 39.326 89 39 2 19 105 20 95 9.37e-13 63.9
MS.gene058766.t1 MTR_6g061070 23.288 292 161 8 23 255 34 321 2.31e-12 66.6
MS.gene058766.t1 MTR_4g119990 24.727 275 146 12 22 242 30 297 6.72e-12 65.1
MS.gene058766.t1 MTR_6g043890 22.932 266 156 10 22 242 30 291 4.66e-11 62.4
MS.gene058766.t1 MTR_1g035510 30.612 98 65 2 23 120 2 96 5.69e-11 61.2
MS.gene058766.t1 MTR_1g021290 38.202 89 54 1 12 100 33 120 8.64e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058766.t1 AT3G18990 44.898 98 51 1 8 105 10 104 8.09e-22 93.2
MS.gene058766.t1 AT3G18990 36.296 135 74 4 133 261 213 341 1.66e-15 75.5
MS.gene058766.t1 AT3G18990 44.898 98 51 1 8 105 10 104 1.46e-21 92.8
MS.gene058766.t1 AT3G18990 36.029 136 75 4 133 262 213 342 3.58e-15 74.7
MS.gene058766.t1 AT1G49475 40.625 96 56 1 20 115 47 141 2.08e-18 80.9
MS.gene058766.t1 AT3G18960 40.000 90 54 0 21 110 7 96 5.97e-18 79.3
MS.gene058766.t1 AT3G18960 37.000 100 60 1 8 107 34 130 8.18e-18 79.3
MS.gene058766.t1 AT4G01580 35.922 103 63 1 8 110 34 133 9.74e-18 79.3
MS.gene058766.t1 AT3G18960 37.000 100 60 1 8 107 34 130 1.19e-17 79.3
MS.gene058766.t1 AT1G49480 36.296 135 74 4 133 261 98 226 5.95e-16 75.1
MS.gene058766.t1 AT1G49480 36.296 135 74 4 133 261 98 226 5.95e-16 75.1
MS.gene058766.t1 AT1G49480 36.296 135 74 4 133 261 98 226 5.95e-16 75.1

Find 39 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTCCCATTAGAATTT+TGG 0.142530 3.1:+729372 None:intergenic
AATGCTCTCCCTCAGAATTT+TGG 0.165314 3.1:+729212 None:intergenic
AAGTGAGAGTCATGAGAAAA+AGG 0.253305 3.1:-729476 MS.gene058766:CDS
TTTGCAGAATACTATTCTAT+AGG 0.313465 3.1:-729664 MS.gene058766:CDS
CATTGCAGATGATGATGATT+TGG 0.324669 3.1:+729162 None:intergenic
TTGCAGAATACTATTCTATA+GGG 0.341018 3.1:-729663 MS.gene058766:CDS
GTCAGCCCTCCTCTAAAATT+TGG 0.348157 3.1:-729864 MS.gene058766:CDS
AGGGGCAACAATCTGTCTTA+TGG 0.381060 3.1:-729109 MS.gene058766:CDS
TTCCTGTGTTTCAAATATGA+AGG 0.394296 3.1:-729631 MS.gene058766:CDS
TTCAAAACTCTAAGTACAAA+TGG 0.425055 3.1:-729550 MS.gene058766:CDS
GGGATTTACTATATTTCAAA+GGG 0.437078 3.1:-729128 MS.gene058766:CDS
AAATTCTGAGGGAGAGCATT+GGG 0.443772 3.1:-729209 MS.gene058766:CDS
CTGTTCCTGATGGTCGTGAT+TGG 0.459514 3.1:-729744 MS.gene058766:CDS
CAGTTCATCCGTTATAGTAT+AGG 0.459617 3.1:+729840 None:intergenic
ACTCTCACTTGTTTCAACCT+TGG 0.466400 3.1:+729489 None:intergenic
CATCTGCAATGATAATATCA+AGG 0.473557 3.1:-729150 MS.gene058766:CDS
GGGGATTTACTATATTTCAA+AGG 0.484674 3.1:-729129 MS.gene058766:CDS
ATTTCCCAATCACGACCATC+AGG 0.484720 3.1:+729739 None:intergenic
AGTGTCATAGTCGTAGAAAA+AGG 0.486595 3.1:-729513 MS.gene058766:CDS
TGATGGTCGTGATTGGGAAA+TGG 0.490310 3.1:-729737 MS.gene058766:CDS
TGTTCCTGATGGTCGTGATT+GGG 0.496959 3.1:-729743 MS.gene058766:CDS
AATTCTAATGGGAAATTATG+TGG 0.499901 3.1:-729368 MS.gene058766:intron
CCCCTTCATATTTGAAACAC+AGG 0.508323 3.1:+729629 None:intergenic
GAATACTATTCTATAGGGTA+TGG 0.523564 3.1:-729658 MS.gene058766:CDS
AAAATTCTGAGGGAGAGCAT+TGG 0.523796 3.1:-729210 MS.gene058766:CDS
TCCTGTGTTTCAAATATGAA+GGG 0.529278 3.1:-729630 MS.gene058766:CDS
AATTTGGTCCTATACTATAA+CGG 0.531388 3.1:-729848 MS.gene058766:CDS
AATGTGCAGTATGTTAATGC+TGG 0.551027 3.1:-729277 MS.gene058766:intron
ATCTGCAATGATAATATCAA+GGG 0.551519 3.1:-729149 MS.gene058766:CDS
CCTGTGTTTCAAATATGAAG+GGG 0.591078 3.1:-729629 MS.gene058766:CDS
TGAGCTGATCAATAAGAAGC+CGG 0.592920 3.1:-729071 MS.gene058766:CDS
TGTAAAGCTCCAAAATTCTG+AGG 0.595481 3.1:-729221 MS.gene058766:CDS
ACATGCTTCTGAAAGAGCAA+AGG 0.603971 3.1:-729437 MS.gene058766:CDS
GTAAAGCTCCAAAATTCTGA+GGG 0.614548 3.1:-729220 MS.gene058766:CDS
TTCATATTTGAAACACAGGA+AGG 0.623348 3.1:+729633 None:intergenic
GCTACAATTACTGTTCCTGA+TGG 0.638622 3.1:-729754 MS.gene058766:CDS
GAAATGGAACTGAAGAAATG+CGG 0.682734 3.1:-729721 MS.gene058766:CDS
TCTGCAATGATAATATCAAG+GGG 0.693916 3.1:-729148 MS.gene058766:CDS
GGATTTACTATATTTCAAAG+GGG 0.704196 3.1:-729127 MS.gene058766:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATGAGTTTATAAAAAGATT+TGG - chr3.1:729096-729115 MS.gene058766:CDS 20.0%
!!! AATCTTTTTATAAACTCATC+TGG + chr3.1:729096-729115 None:intergenic 20.0%
!!! ACATTTACTTTTGTAACAAA+TGG - chr3.1:729199-729218 MS.gene058766:CDS 20.0%
!!! AGAATTTTGGAACAAAAATA+AGG + chr3.1:729510-729529 None:intergenic 20.0%
!!! ATAATTTCCCATTAGAATTT+TGG + chr3.1:729523-729542 None:intergenic 20.0%
!!! GAATTTTGGAACAAAAATAA+GGG + chr3.1:729509-729528 None:intergenic 20.0%
!!! GTTTTTTGGATTAAATTCTT+TGG + chr3.1:729487-729506 None:intergenic 20.0%
!!! TTTTGTTCCAAAATTCTAAT+GGG - chr3.1:729513-729532 MS.gene058766:CDS 20.0%
!!! TTTTTGTTCCAAAATTCTAA+TGG - chr3.1:729512-729531 MS.gene058766:CDS 20.0%
! AATTCTAATGGGAAATTATG+TGG - chr3.1:729524-729543 MS.gene058766:CDS 25.0%
! AATTTGGTCCTATACTATAA+CGG - chr3.1:729044-729063 MS.gene058766:CDS 25.0%
! ATCTGCAATGATAATATCAA+GGG - chr3.1:729743-729762 MS.gene058766:CDS 25.0%
! GGATTTACTATATTTCAAAG+GGG - chr3.1:729765-729784 MS.gene058766:CDS 25.0%
! GGGATTTACTATATTTCAAA+GGG - chr3.1:729764-729783 MS.gene058766:CDS 25.0%
! TTCAAAACTCTAAGTACAAA+TGG - chr3.1:729342-729361 MS.gene058766:intron 25.0%
! TTGCAGAATACTATTCTATA+GGG - chr3.1:729229-729248 MS.gene058766:CDS 25.0%
! TTTGCAGAATACTATTCTAT+AGG - chr3.1:729228-729247 MS.gene058766:CDS 25.0%
!!! GAACAAAAATAAGGGTTTTT+TGG + chr3.1:729501-729520 None:intergenic 25.0%
!!! TTTGTACTTAGAGTTTTGAA+TGG + chr3.1:729342-729361 None:intergenic 25.0%
CATCTGCAATGATAATATCA+AGG - chr3.1:729742-729761 MS.gene058766:CDS 30.0%
GAATACTATTCTATAGGGTA+TGG - chr3.1:729234-729253 MS.gene058766:CDS 30.0%
GGGGATTTACTATATTTCAA+AGG - chr3.1:729763-729782 MS.gene058766:CDS 30.0%
TCCTGTGTTTCAAATATGAA+GGG - chr3.1:729262-729281 MS.gene058766:CDS 30.0%
TCTGCAATGATAATATCAAG+GGG - chr3.1:729744-729763 MS.gene058766:CDS 30.0%
TTCCTGTGTTTCAAATATGA+AGG - chr3.1:729261-729280 MS.gene058766:CDS 30.0%
! AGTATAGGACCAAATTTTAG+AGG + chr3.1:729040-729059 None:intergenic 30.0%
! TTCATATTTGAAACACAGGA+AGG + chr3.1:729262-729281 None:intergenic 30.0%
AAGTGAGAGTCATGAGAAAA+AGG - chr3.1:729416-729435 MS.gene058766:CDS 35.0%
AATGTGCAGTATGTTAATGC+TGG - chr3.1:729615-729634 MS.gene058766:CDS 35.0%
AGTGAGAGTCATGAGAAAAA+GGG - chr3.1:729417-729436 MS.gene058766:CDS 35.0%
AGTGTCATAGTCGTAGAAAA+AGG - chr3.1:729379-729398 MS.gene058766:CDS 35.0%
CAGTTCATCCGTTATAGTAT+AGG + chr3.1:729055-729074 None:intergenic 35.0%
CCTGTGTTTCAAATATGAAG+GGG - chr3.1:729263-729282 MS.gene058766:CDS 35.0%
GAAATGGAACTGAAGAAATG+CGG - chr3.1:729171-729190 MS.gene058766:CDS 35.0%
GTAAAGCTCCAAAATTCTGA+GGG - chr3.1:729672-729691 MS.gene058766:CDS 35.0%
TAATGGGAAATTATGTGGTG+AGG - chr3.1:729529-729548 MS.gene058766:CDS 35.0%
TGTAAAGCTCCAAAATTCTG+AGG - chr3.1:729671-729690 MS.gene058766:CDS 35.0%
! ATTGTGACTTTTAGCACATC+CGG + chr3.1:729843-729862 None:intergenic 35.0%
! CATTGCAGATGATGATGATT+TGG + chr3.1:729733-729752 None:intergenic 35.0%
!! ATAGGACCAAATTTTAGAGG+AGG + chr3.1:729037-729056 None:intergenic 35.0%
!! TAGGACCAAATTTTAGAGGA+GGG + chr3.1:729036-729055 None:intergenic 35.0%
!!! TCAGAATTTTGGAGCTTTAC+AGG + chr3.1:729672-729691 None:intergenic 35.0%
ACTCTCACTTGTTTCAACCT+TGG + chr3.1:729406-729425 None:intergenic 40.0%
GCTACAATTACTGTTCCTGA+TGG - chr3.1:729138-729157 MS.gene058766:CDS 40.0%
TAGTCGTAGAAAAAGGTCCA+AGG - chr3.1:729386-729405 MS.gene058766:CDS 40.0%
TGAGCTGATCAATAAGAAGC+CGG - chr3.1:729821-729840 MS.gene058766:CDS 40.0%
! AAAATTCTGAGGGAGAGCAT+TGG - chr3.1:729682-729701 MS.gene058766:CDS 40.0%
! AATGCTCTCCCTCAGAATTT+TGG + chr3.1:729683-729702 None:intergenic 40.0%
! ACATGCTTCTGAAAGAGCAA+AGG - chr3.1:729455-729474 MS.gene058766:CDS 40.0%
! CCCCTTCATATTTGAAACAC+AGG + chr3.1:729266-729285 None:intergenic 40.0%
!! AAATTCTGAGGGAGAGCATT+GGG - chr3.1:729683-729702 MS.gene058766:CDS 40.0%
AGGGGCAACAATCTGTCTTA+TGG - chr3.1:729783-729802 MS.gene058766:CDS 45.0%
ATTTCCCAATCACGACCATC+AGG + chr3.1:729156-729175 None:intergenic 45.0%
TGATGGTCGTGATTGGGAAA+TGG - chr3.1:729155-729174 MS.gene058766:CDS 45.0%
TGTTCCTGATGGTCGTGATT+GGG - chr3.1:729149-729168 MS.gene058766:CDS 45.0%
!! GTCAGCCCTCCTCTAAAATT+TGG - chr3.1:729028-729047 MS.gene058766:CDS 45.0%
CTGTTCCTGATGGTCGTGAT+TGG - chr3.1:729148-729167 MS.gene058766:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 729024 729890 729024 ID=MS.gene058766
chr3.1 mRNA 729024 729890 729024 ID=MS.gene058766.t1;Parent=MS.gene058766
chr3.1 exon 729369 729890 729369 ID=MS.gene058766.t1.exon1;Parent=MS.gene058766.t1
chr3.1 CDS 729369 729890 729369 ID=cds.MS.gene058766.t1;Parent=MS.gene058766.t1
chr3.1 exon 729024 729290 729024 ID=MS.gene058766.t1.exon2;Parent=MS.gene058766.t1
chr3.1 CDS 729024 729290 729024 ID=cds.MS.gene058766.t1;Parent=MS.gene058766.t1
Gene Sequence

>MS.gene058766

ATGTGTCAGCCCTCCTCTAAAATTTGGTCCTATACTATAACGGATGAACTGTTATCACTGCAGAGAATTCCAGATGAGTTTATAAAAAGATTTGGAAATGAACTCAAGAATGTTGCTACAATTACTGTTCCTGATGGTCGTGATTGGGAAATGGAACTGAAGAAATGCGGCAACGACATTTACTTTTGTAACAAATGGCAACAGTTTGCAGAATACTATTCTATAGGGTATGGTTCCTTCCTGTGTTTCAAATATGAAGGGGATTCAAAGTTTAGTGTCGTTATATTTGATGTAACTTCTGTTGAGATTTGCTATCCATTCAAAACTCTAAGTACAAATGGAGAAACAAACACAAAGTGTCATAGTCGTAGAAAAAGGTCCAAGGTTGAAACAAGTGAGAGTCATGAGAAAAAGGGCAAAAGCATGTCTAAACATGCTTCTGAAAGAGCAAAGGAAGCTGCCAAAGAATTTAATCCAAAAAACCCTTATTTTTGTTCCAAAATTCTAATGGGAAATTATGTGGTGAGGATTTTACTTTAAAGCAAATGTTGAGTTTTTTATTTATGTTATCACTTTAATGATCTCTCATTTAATGTGCAGTATGTTAATGCTGGTTTTGCTTCAAAGCATCTGAATCTAAATGTTCCTGTAAAGCTCCAAAATTCTGAGGGAGAGCATTGGGAAGTTTCTTGTGCAATGCATGATGCCAAATCATCATCATCTGCAATGATAATATCAAGGGGATTTACTATATTTCAAAGGGGCAACAATCTGTCTTATGGAGATTATTGTGTGTTTGAGCTGATCAATAAGAAGCCGGATGTGCTAAAAGTCACAATGTTTCGTGTGATTGACTATTGCGATTAA

Protein sequence

>MS.gene058766.t1

MCQPSSKIWSYTITDELLSLQRIPDEFIKRFGNELKNVATITVPDGRDWEMELKKCGNDIYFCNKWQQFAEYYSIGYGSFLCFKYEGDSKFSVVIFDVTSVEICYPFKTLSTNGETNTKCHSRRKRSKVETSESHEKKGKSMSKHASERAKEAAKEFNPKNPYFCSKILMGNYVYVNAGFASKHLNLNVPVKLQNSEGEHWEVSCAMHDAKSSSSAMIISRGFTIFQRGNNLSYGDYCVFELINKKPDVLKVTMFRVIDYCD