AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059476

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059476.t1 MTR_5g005940 38.035 631 353 14 53 654 76 697 6.92e-99 318
MS.gene059476.t1 MTR_1g104870 36.282 667 386 15 14 650 39 696 7.04e-99 318
MS.gene059476.t1 MTR_7g094910 34.877 648 382 16 45 662 60 697 1.54e-81 272
MS.gene059476.t1 MTR_7g094900 35.897 585 346 14 97 662 142 716 1.38e-80 270
MS.gene059476.t1 MTR_5g005940 36.477 562 312 14 26 550 39 592 2.92e-74 250
MS.gene059476.t1 MTR_7g094910 35.263 570 336 12 45 589 60 621 5.76e-71 242
MS.gene059476.t1 MTR_3g008350 28.884 457 263 18 33 461 42 464 1.26e-25 112
MS.gene059476.t1 MTR_1g012360 27.158 475 285 21 68 494 68 529 8.24e-16 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059476.t1 AT5G13060 33.583 667 392 16 17 650 77 725 5.17e-88 290
MS.gene059476.t1 AT5G19330 34.762 630 373 14 44 644 72 692 5.69e-87 287
MS.gene059476.t1 AT5G19330 30.506 613 333 14 57 643 86 631 7.30e-52 190

Find 121 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGTTGCTCTAACATTAA+AGG 0.177108 1.3:-11021038 MS.gene059476:CDS
CAAGTTGGCCAGTAAATTCT+TGG 0.274559 1.3:-11023433 MS.gene059476:CDS
ATCGACTTCTGAACTTAAAT+AGG 0.307874 1.3:+11023652 None:intergenic
AACACGAATTGCTTTGGCTT+TGG 0.334035 1.3:-11023675 MS.gene059476:CDS
ATAAGAATACTGAGCTTTCC+TGG 0.338428 1.3:+11024887 None:intergenic
TCATCGTCTTGCAGATAATA+AGG 0.339116 1.3:-11024205 MS.gene059476:intron
TACTTAGCGTGGAAACTATA+TGG 0.342160 1.3:-11021084 MS.gene059476:CDS
GACGTTATATTTCTTATTGA+AGG 0.346191 1.3:-11023036 MS.gene059476:intron
TTCGTTGCTCTAACATTAAA+GGG 0.350794 1.3:-11021037 MS.gene059476:CDS
CAACTATGCTAGATAGTATA+AGG 0.352751 1.3:-11027243 MS.gene059476:intron
ATCCTCCTTGGAGGAGTCTC+TGG 0.355174 1.3:+11024030 None:intergenic
TCATTAGCGGAAAAGATTAA+TGG 0.357540 1.3:-11023834 MS.gene059476:CDS
ATTAGAACCACTTCAGTTAT+CGG 0.364657 1.3:+11028323 None:intergenic
CTAAACTGCCACTGCGTAAG+AGG 0.378205 1.3:+11020752 None:intergenic
GTAAGTGCCTTTGTCAATAA+AGG 0.380566 1.3:+11024928 None:intergenic
ATAAAGGTATAAGACCTCTT+TGG 0.387340 1.3:+11024944 None:intergenic
AGCTTCCGATCCCTCCTTAG+AGG 0.391644 1.3:+11023470 None:intergenic
ATCGAAACAAATCAAATGCT+TGG 0.395849 1.3:+11023868 None:intergenic
CTCCTCCAAGGAGGATATCT+TGG 0.404179 1.3:-11024023 MS.gene059476:CDS
GCTACTTGGCTCATCCTCTA+AGG 0.406347 1.3:-11023484 MS.gene059476:CDS
TTAGAACCACTTCAGTTATC+GGG 0.408364 1.3:+11028324 None:intergenic
TTCTATATCTATCAGATTGT+AGG 0.413639 1.3:-11025761 MS.gene059476:intron
CTCCTCATCATACTCTGTTA+TGG 0.426496 1.3:+11028555 None:intergenic
TACCTCATTAATTCTGTAAG+TGG 0.440870 1.3:+11025256 None:intergenic
AGATGGGGCCAAGAATTTAC+TGG 0.441095 1.3:+11023425 None:intergenic
ATTCGTGTTTGAAAATCATT+CGG 0.442477 1.3:+11023689 None:intergenic
CTACTTTGAGAGCCAACGTT+TGG 0.442715 1.3:-11025145 MS.gene059476:CDS
CAGGACTCGGATTGTCAAGT+TGG 0.452315 1.3:-11024312 MS.gene059476:CDS
TAAATTCTTGGCCCCATCTC+CGG 0.457915 1.3:-11023421 MS.gene059476:CDS
AATATTGTACCTTCCATTGT+GGG 0.461806 1.3:+11025088 None:intergenic
GTTAAGATCTACTGTGGGTC+TGG 0.463107 1.3:+11020718 None:intergenic
GATGCACAATATCATCTAGT+AGG 0.464401 1.3:+11027109 None:intergenic
TACTTCTTTATCAATACATC+CGG 0.470787 1.3:-11023397 MS.gene059476:intron
TCGAAACAAATCAAATGCTT+GGG 0.476037 1.3:+11023869 None:intergenic
TGCAGATGGAATTCCATCCT+TGG 0.479025 1.3:-11027136 MS.gene059476:CDS
ATCTATCAGATTGTAGGTCT+CGG 0.479989 1.3:-11025755 MS.gene059476:intron
ATATTAAGAATGGCATTTCA+CGG 0.482955 1.3:-11028490 MS.gene059476:intron
TGCAGTGCCCGCTGGTACAG+TGG 0.483512 1.3:+11021514 None:intergenic
GAGTTGCTTACTGAGCTACT+TGG 0.486455 1.3:-11023498 MS.gene059476:CDS
ATCATGTTGTCAATAAAGTC+AGG 0.495057 1.3:+11020802 None:intergenic
CAAGTTGGTATTGTGAATGA+AGG 0.499666 1.3:-11024297 MS.gene059476:CDS
TCGAATAATAAATGCTTCAA+AGG 0.502494 1.3:-11022873 MS.gene059476:CDS
TTGTTTAGATTTATTTACAC+TGG 0.503233 1.3:-11021358 MS.gene059476:intron
CTTATTATGTTTCTTATCTG+AGG 0.504191 1.3:-11024869 MS.gene059476:intron
ATTGACACTTGAATCATTAG+CGG 0.504484 1.3:-11023847 MS.gene059476:CDS
GAGAAACTATGAAGAGTTGA+GGG 0.505799 1.3:-11020993 MS.gene059476:CDS
CATTCGGCAAACTCCACATT+CGG 0.507417 1.3:+11023705 None:intergenic
GATAAAGAAGTAGCCGGAGA+TGG 0.508149 1.3:+11023408 None:intergenic
ATCCACTTACAGAATTAATG+AGG 0.509465 1.3:-11025258 MS.gene059476:intron
AGTCGTGAGGATATCAATAG+AGG 0.514618 1.3:+11027282 None:intergenic
ATAAAGAAGTAGCCGGAGAT+GGG 0.514779 1.3:+11023409 None:intergenic
CATCACCAAGATATCCTCCT+TGG 0.515233 1.3:+11024018 None:intergenic
TTGTACCTTCCATTGTGGGT+TGG 0.521112 1.3:+11025092 None:intergenic
GTCTGCTAATGAAGGAGTCC+AGG 0.521443 1.3:-11024905 MS.gene059476:CDS
GAATATTGTACCTTCCATTG+TGG 0.525011 1.3:+11025087 None:intergenic
AAGAGTAGCTTGCTTTGCGT+TGG 0.526568 1.3:-11027085 MS.gene059476:CDS
CTCATCCTCTAAGGAGGGAT+CGG 0.529278 1.3:-11023475 MS.gene059476:CDS
CTGTGAAAATAAGATTGCTC+AGG 0.533457 1.3:-11021254 MS.gene059476:intron
TGCTGCGGCTTTATTCAAGT+TGG 0.534737 1.3:-11023448 MS.gene059476:CDS
CACCAAGATATCCTCCTTGG+AGG 0.535091 1.3:+11024021 None:intergenic
CTCAACTGTTACACCGAATG+TGG 0.535467 1.3:-11023718 MS.gene059476:CDS
TATAGCATTAGAATTATAAG+TGG 0.536156 1.3:+11025725 None:intergenic
TCTAGTAGGTGAACCAAGGA+TGG 0.539296 1.3:+11027123 None:intergenic
ACAAGATGTCTAGCTAAGGC+TGG 0.541047 1.3:+11028350 None:intergenic
CAAACGTTGGCTCTCAAAGT+AGG 0.542198 1.3:+11025146 None:intergenic
TAGATCATGCTTATCCAAAG+AGG 0.547059 1.3:-11024958 MS.gene059476:CDS
ACTATGAAGAGTTGAGGGAA+AGG 0.551507 1.3:-11020988 MS.gene059476:intron
GAGTGCTATGGTTACTAAGT+CGG 0.552815 1.3:-11022846 MS.gene059476:CDS
TGAGAAACTATGAAGAGTTG+AGG 0.557066 1.3:-11020994 MS.gene059476:CDS
TATTGTGAATGAAGGCTTGG+TGG 0.558315 1.3:-11024289 MS.gene059476:CDS
AAATTATATTCTCTGCAAGT+AGG 0.560105 1.3:-11024243 MS.gene059476:CDS
TTGCAGAAAGAATTGTGAAG+AGG 0.560350 1.3:-11028394 MS.gene059476:intron
TCTTATACCTTTATTGACAA+AGG 0.563122 1.3:-11024935 MS.gene059476:CDS
ACGGAGCTTGTGAGATAGTG+AGG 0.567161 1.3:-11025306 MS.gene059476:CDS
GTGAAGAGGATGGTGGACTT+AGG 0.572667 1.3:-11028380 MS.gene059476:CDS
GCACTTACGTCTGCTAATGA+AGG 0.575276 1.3:-11024913 MS.gene059476:CDS
TTCTTAATATAAAATTAGCA+CGG 0.580057 1.3:+11028503 None:intergenic
AATCAAATCAAGCAAATCGA+TGG 0.582383 1.3:+11025122 None:intergenic
TGTATTGATAAAGAAGTAGC+CGG 0.582481 1.3:+11023402 None:intergenic
TTTGTGGTTAAGATCTACTG+TGG 0.585702 1.3:+11020712 None:intergenic
AGCAACCAAATGATTAACTA+CGG 0.585894 1.3:-11028646 None:intergenic
CGCACAGAATGTACTTAGCG+TGG 0.586567 1.3:-11021095 MS.gene059476:intron
GTGTACAAGTTTATCATGCT+AGG 0.592047 1.3:-11024059 MS.gene059476:CDS
GGTGGAATTACTGAAGGTGT+TGG 0.593973 1.3:-11024271 MS.gene059476:CDS
CAGATGATCTATAATCTAGA+CGG 0.595673 1.3:-11025325 MS.gene059476:CDS
GGAGTACAGTTCAAAGATTG+AGG 0.596794 1.3:-11028536 MS.gene059476:CDS
ACTACGTGTGCAGTGCCCGC+TGG 0.598387 1.3:+11021506 None:intergenic
TGTGTCCCCGATAACTGAAG+TGG 0.599893 1.3:-11028330 MS.gene059476:CDS
AAAGACCAACCCACAATGGA+AGG 0.601200 1.3:-11025097 MS.gene059476:intron
TTCAAAGGTGTTGAGTGCTA+TGG 0.603447 1.3:-11022858 MS.gene059476:CDS
TGGTATTGTGAATGAAGGCT+TGG 0.604614 1.3:-11024292 MS.gene059476:CDS
ACTTGGCTCATCCTCTAAGG+AGG 0.604968 1.3:-11023481 MS.gene059476:CDS
ACCTAAAATACACCAACAGA+TGG 0.610698 1.3:-11020779 MS.gene059476:CDS
GGAGTCCAGAGACTCCTCCA+AGG 0.616675 1.3:-11024035 MS.gene059476:CDS
GGACACAAGATGTCTAGCTA+AGG 0.619572 1.3:+11028346 None:intergenic
GGAGGGATCGGAAGCTGCTG+CGG 0.621384 1.3:-11023463 MS.gene059476:CDS
TAGAACCACTTCAGTTATCG+GGG 0.625659 1.3:+11028325 None:intergenic
CTTGGCTCATCCTCTAAGGA+GGG 0.627160 1.3:-11023480 MS.gene059476:CDS
GTAAGAGGTGTACCATCTGT+TGG 0.629013 1.3:+11020767 None:intergenic
TTGTAAAGACCAACCCACAA+TGG 0.630882 1.3:-11025101 MS.gene059476:CDS
ACGTTGGCTCTCAAAGTAGG+TGG 0.633070 1.3:+11025149 None:intergenic
TTGTGGTTAAGATCTACTGT+GGG 0.637838 1.3:+11020713 None:intergenic
GCAAATCGATGGCCAAACGT+TGG 0.640110 1.3:+11025133 None:intergenic
GTAGATACCACTGTACCAGC+GGG 0.647731 1.3:-11021521 MS.gene059476:CDS
ATCATCTAGTAGGTGAACCA+AGG 0.648799 1.3:+11027119 None:intergenic
AAGAATTGTGAAGAGGATGG+TGG 0.651093 1.3:-11028387 MS.gene059476:CDS
TAAAGAAGTAGCCGGAGATG+GGG 0.655484 1.3:+11023410 None:intergenic
AGAAAGAATTGTGAAGAGGA+TGG 0.656473 1.3:-11028390 MS.gene059476:CDS
TACAAGTTTATCATGCTAGG+TGG 0.658188 1.3:-11024056 MS.gene059476:CDS
GAACCATGAAAATGCTACAC+AGG 0.659261 1.3:-11027049 MS.gene059476:intron
ATATCCTCACGACTCTTGCG+AGG 0.664239 1.3:-11027273 MS.gene059476:CDS
TACCATAACAGAGTATGATG+AGG 0.683678 1.3:-11028557 MS.gene059476:CDS
GCTTGTGAGATAGTGAGGAG+AGG 0.683896 1.3:-11025301 MS.gene059476:CDS
TTGTCCTCGCAAGAGTCGTG+AGG 0.687559 1.3:+11027269 None:intergenic
GGTAGATACCACTGTACCAG+CGG 0.696611 1.3:-11021522 MS.gene059476:CDS
GAGGATGGTGGACTTAGGTG+TGG 0.697058 1.3:-11028375 MS.gene059476:CDS
AGGCTTGGTGGAATTACTGA+AGG 0.698166 1.3:-11024277 MS.gene059476:CDS
GTCCAGAGACTCCTCCAAGG+AGG 0.702113 1.3:-11024032 MS.gene059476:CDS
ATGGTACACCTCTTACGCAG+TGG 0.704422 1.3:-11020760 MS.gene059476:CDS
TGGAAACACATATGTCAGTG+TGG 0.704970 1.3:-11021338 MS.gene059476:CDS
CACGCAACTTCACAACTCTG+TGG 0.713617 1.3:-11025693 MS.gene059476:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.3 gene 11020726 11028659 11020726 ID=MS.gene059476
chr1.3 mRNA 11020726 11028659 11020726 ID=MS.gene059476.t1;Parent=MS.gene059476
chr1.3 exon 11028491 11028659 11028491 ID=MS.gene059476.t1.exon1;Parent=MS.gene059476.t1
chr1.3 CDS 11028491 11028659 11028491 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11028292 11028410 11028292 ID=MS.gene059476.t1.exon2;Parent=MS.gene059476.t1
chr1.3 CDS 11028292 11028410 11028292 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11027244 11027401 11027244 ID=MS.gene059476.t1.exon3;Parent=MS.gene059476.t1
chr1.3 CDS 11027244 11027401 11027244 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11027050 11027161 11027050 ID=MS.gene059476.t1.exon4;Parent=MS.gene059476.t1
chr1.3 CDS 11027050 11027161 11027050 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11025694 11025768 11025694 ID=MS.gene059476.t1.exon5;Parent=MS.gene059476.t1
chr1.3 CDS 11025694 11025768 11025694 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11025259 11025350 11025259 ID=MS.gene059476.t1.exon6;Parent=MS.gene059476.t1
chr1.3 CDS 11025259 11025350 11025259 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11025098 11025173 11025098 ID=MS.gene059476.t1.exon7;Parent=MS.gene059476.t1
chr1.3 CDS 11025098 11025173 11025098 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11024870 11024977 11024870 ID=MS.gene059476.t1.exon8;Parent=MS.gene059476.t1
chr1.3 CDS 11024870 11024977 11024870 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11024206 11024331 11024206 ID=MS.gene059476.t1.exon9;Parent=MS.gene059476.t1
chr1.3 CDS 11024206 11024331 11024206 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11024019 11024087 11024019 ID=MS.gene059476.t1.exon10;Parent=MS.gene059476.t1
chr1.3 CDS 11024019 11024087 11024019 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11023830 11023892 11023830 ID=MS.gene059476.t1.exon11;Parent=MS.gene059476.t1
chr1.3 CDS 11023830 11023892 11023830 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11023624 11023747 11023624 ID=MS.gene059476.t1.exon12;Parent=MS.gene059476.t1
chr1.3 CDS 11023624 11023747 11023624 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11023398 11023525 11023398 ID=MS.gene059476.t1.exon13;Parent=MS.gene059476.t1
chr1.3 CDS 11023398 11023525 11023398 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11023037 11023103 11023037 ID=MS.gene059476.t1.exon14;Parent=MS.gene059476.t1
chr1.3 CDS 11023037 11023103 11023037 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11022838 11022920 11022838 ID=MS.gene059476.t1.exon15;Parent=MS.gene059476.t1
chr1.3 CDS 11022838 11022920 11022838 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11021464 11021543 11021464 ID=MS.gene059476.t1.exon16;Parent=MS.gene059476.t1
chr1.3 CDS 11021464 11021543 11021464 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11021255 11021372 11021255 ID=MS.gene059476.t1.exon17;Parent=MS.gene059476.t1
chr1.3 CDS 11021255 11021372 11021255 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11020989 11021110 11020989 ID=MS.gene059476.t1.exon18;Parent=MS.gene059476.t1
chr1.3 CDS 11020989 11021110 11020989 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
chr1.3 exon 11020726 11020828 11020726 ID=MS.gene059476.t1.exon19;Parent=MS.gene059476.t1
chr1.3 CDS 11020726 11020828 11020726 ID=cds.MS.gene059476.t1;Parent=MS.gene059476.t1
Gene Sequence

>MS.gene059476

ATGATTAACTACGGTAATAGAAATACTCAAAGTCTTGATGATGACAATATTCGCTCGTTTACAGAATTTCTGAATGATGTTACCATAACAGAGTATGATGAGGAGTACAGTTCAAAGATTGAGGAGTTCAAGAACCGTGCTAATTTTATATTAAGAATGGCATTTCACGGTAATGCACTGTTTTTATTTTCAACTGTTGCATGAAATGTAAAACAGCTGTTTCATCATGAACGATGTTCCTTTTTGCAGAAAGAATTGTGAAGAGGATGGTGGACTTAGGTGTGGTTCCAGCCTTAGCTAGACATCTTGTGTCCCCGATAACTGAAGTGGTTCTAATAACTCTAAGAGCACTGAAGAATTTTGCTAAGGTAAGTTTTCATTTGCCATTTTTTGTTAGTTGTTGTGGTCAAGGTTTTAAATAACCGTCGTAGTCAAAAGTCGCGATTTCGGCTGTTACCGATGTTGCGTTACGAATTGTGGTTGTCGCGGTGTGACAAATGAGTCAGTGTCCCGTGCATTTCAGGCACCTGTGTGTGCCGTATTTTAAGGAGTGTTCGTGTTTCATTGTTAAAAAATTAATGTAAGAGTTGAAGTCTATAAAGCATGCACACACACACACACACCAGATATGCACAAACATTGAAATGTCGGTAGCGTAAATGATTTATGAAAATGACACGAGTGACCAACGGGTGTCAGACACTGGGGCACTCCTAATTCGAGGAGTGACCGTGTTTCATTGTTCGAATGTAAATGAAAGAGTTAAGTTCTATGAAGCACAAACACAAACAGAAACCGGATATGACACTAACACTTAGGGTCTGTTTGGATGGAGGGTTTAGGAGGGGAGGGGAAGGGAGGGGAGAGTTTACTTTTCATTTTTAAATTACGGACACTATAAAATGTTTTTTAAAAAGTTAACATGTTAACATGATAAATGATCATATAATACTTCAATCACATTTAATTTATTTTAGAAAAACATGTTATTTAGTCCGTACTTTAAAAATGAAAAAAGTAAACCCTCCCCTCCCTTCCCCTCCTAAAACCTCAAGCCAAACCCACCCTTAAGGTTTTAGGTAAAGATGTGATATGCAATCTCATTTACTCAATGTATAGACTCTTTTGGAAGTCCCAACTTTGGCATCAATTAATTGCTACTTATTCCTTTTAATTGTTCCATTTGTTTTGATTTATTTGTTTTAATTCTTTCATTACAACTGTTAGCTAACGATGCTCCTATTACATTTGCTTGCAGCAATATCAAGAGCAATTTGATGAGACTCGTGCTGTATCACAAATAATTAATTTTTTAAACAATAGTCAAAATGAAGCAAAACACAGTTTGATTTACTCCTCTATTGATATCCTCACGACTCTTGCGAGGACAAGCACAACTATGCTAGATAGTATAAGGTTTAGTTTTTCAACCAATCCTGCTATTAATTTGAATTAACTTGATTTGATTTCTTTTTAATCCAATTTGAATTTTGTGTAGGATTGCAGATGGAATTCCATCCTTGGTTCACCTACTAGATGATATTGTGCATCAAAGAGTAGCTTGCTTTGCGTTGGAAGTCCTTTCCACGAACCATGAAAATGCTACACAGGTAGCACTTTTTTTTTTTTGTTGAACATCAAACAAATTTATTTGATATGAATGAGTAAAGCAAATTTCAAACCGATTTGTAAAACTATAAAAATTGTTAGATTATCAATTATCAAAATATATTGACATCATAGTAAGAAAATGATCATGAGACTAAAGAGCTTATGAGTCTTACCCGTGATGATAAATTTCTTAAGATCGTCTCTAACCTGTTTTTACTTTTTTTTTTTTTTTTCCTTCTATGTTCTTCTTCCTTTTTTTTTTTTAATTATCTCTATTTGTTCTTTTTTCTTTTGTGGAATCGATAAATGGGAGTATAAATTGAGAGAGAAGAGAAATTGAATTGGCTTTTTGATGACAGAAAGTGAGGTTAAAGGTTGAATATGAAAAAGGAGGATTTTTGAGGTAAAGGTGATGATCCTCAGCCAAACTCTTATCCCACTTTTATTTCCTTCAATGGCATTCAATATAATAACACCAGATCTTCTCCACCAACGTTGTTGCAAGGACCAATTTGGTGCCTTCCATCCAGATTGAAAACTGGATGTTCTCGACTACTGCTACTTCTTTTAGTTGTCCCATGAATTATCCTTTTAGCTTATCAAGGTTCTTCGACTATGTATCAACTTCAATAAAACTTTCTGCTCCTTATGGTAATAAGATCAAGTTTCCTTCCCTCTAGGTTCTTCCTTGCCAAAATCTCCTTGAACGAAATCCCCAGCTACTTCTTAGGGATGAAGACATCCCCCACCTTCCATAAAGAGTAGACGGTTATGTTCTCTAGGAAATTTGTGATAAAAAATTATGTGATGGCTCCTCTCCAAACAAGCTGTCGTGAAGTTGACGAAAAAGGTTTCTGATTGTTACCTAGACTCCATTATTAAGAGAATTAACACATCATTTCACAAATAACTTAACTTTTATCATCTACAGGAGATTGTACCTTTTGTGTTAGAACTTAATGAATCACACACACACTTACAAGAGGGAAAGGAAATCAGAGGGAAATCTTAATTGGTGGTCATGCACTTAACTTCTTCATTCATTGTCACAATGTAAAAACTTATACATCAAACTGGTTGGGCTTACAAGTAACTAGAGAGGCCCAACAAATCATAACCAAATTAGTTAGTTAGTTCCTTGCGCACTAAGCAACCTAACTAACTTGGTTATCTAGATAACTGCACATCAAGTAACATAACTAACCTAACTACTATTTGAATTGCACTATAAACAATAATAATTTGAATGACATGTAGTAATACAATTCTATATCTATCAGATTGTAGGTCTCGGTGCTGTTCCACTTATAATTCTAATGCTATATCGTGAAGACACGCAACTTCACAACTCTGTGGTATGTATTTGAAGTCATCTGTTTATGAATCTTATATTTGTAAGCTACTTCCTCTATTTAAGTGTCGTATTTTCAGAATTTTTTGTTTTTTAAAATTTCAAAGTAGTATTCGATGTTGTTTTCTCAAAATTATCTGTGCTTATTTATTTCAGAGAGATAAATAAGTGAGTTGAGATGATAAATAATTAAGGGTAATATAAAAAATAGATAAAATTATTCATAAGATGTAGCAAAATGTAACAGCTTCATTGGTTTGTGTAACCAGAAGTGAGTTAAAAAGGAACAGATGAGTATTCAATATTAAACTTTGTATTATCTAACATTTATAGTGAAATGGTTGCAGTTTCAGATGATCTATAATCTAGACGGAGCTTGTGAGATAGTGAGGAGAGGTTATCTTGTTGAAGCTTTTCATCCACTTACAGAATTAATGAGGTATTTATACTCTTTCCTTTGATTTTCGAAATTATGTCTGATGATTAGTTATGTTCCAATTAATAAATTGTGCTTTATTAACCAGCTCCACCTACTTTGAGAGCCAACGTTTGGCCATCGATTTGCTTGATTTGATTGTAAAGACCAACCCACAATGGAAGGTACAATATTCTTTTTGCTTGAATAACAAAAATATTTTTGATGTATTATTTAGTTACAAGAAACTGACAATTGGTCGTATAATGGAATGTACATGTTCTAACTGATTTTTTATTTTATAGATCATGCTTATCCAAAGAGGTCTTATACCTTTATTGACAAAGGCACTTACGTCTGCTAATGAAGGAGTCCAGGAAAGCTCAGTATTCTTATTATGTTTCTTATCTGAGGTCATTATCCGTTCCTTCTTTTATTTTCTACATAACATCTGCAAGTTTGGGATGTATGTAATTTCACGAACAAAATTTGCTTAAAAAGTACTCAAGTTCAAATTGGACAAAACACTTCATCATCTCTTTTTAAAATAGTGAATGAGCATTTAAGTAACTTTGTTTTTTTCTCTGAAAAATAATAATTCTTAATTCATCCCATTAATGACATAAGAAGCTTCCACATCAGTCCACCATCTAGTCTTTCTTCTATCCATACTGCTTGTTGTTGGTGCCGATGATAACAAATCTAATGAAGACAACTATGGTAAGGATCTTATAGCGGGTTCATCATGGAAATAGATACAAAAGAACCCCTAAAAAAGTCAAACCAAATCCCTAGGCAAAACCCACTCCCATCCTAACAATCATATACCACACCTTAGAGGCCACTCCGCACGTAACAAAGAGATGATTAACAAACTCGGTAGAAACCCCACAATGAGCACAGACAGAACAAGCAGGACTCAGATAATCATAAATTATTTTTCAATTTCAGGACTCGGATTGTCAAGTTGGTATTGTGAATGAAGGCTTGGTGGAATTACTGAAGGTGTTGGACACAAAATTATATTCTCTGCAAGTAGGTGCTGCTTTTACTCTTCATCGTCTTGCAGATAATAAGGTATTATGATATTACTAAATTTTGAATTTAGTAAATGTCTTGATTGGTTTAAATACCGATTATTGTAAACAAGGGAATTAACTATCTATTTTTTATTTTATTTGTTCCCCCTCTATAGGACAATGTGTACAAGTTTATCATGCTAGGTGGAGTCCAGAGACTCCTCCAAGGAGGATATCTTGGTGATGTAAGTTTTTTTCTAAATGTCTATTTTTATCTTTTTAAAATACTTCATGGGTGTTGTTACTTTAGGAATTGGAGGAAAATAATTTCAGATCTGTATTAACCTACATATATATGGTTGATATTGCAGACCCAAGCATTTGATTTGTTTCGATTGACACTTGAATCATTAGCGGAAAAGATTAATGGACATGTATGTCATGTTTCTATTTATTTTTATTTTTGTGTAATTAGTATGTTGTGTTCAAACTTGTAGTTATTTTTTATTCTTGTAGGTACTAACTCAACTGTTACACCGAATGTGGAGTTTGCCGAATGATTTTCAAACACGAATTGCTTTGGCTTTGGCCTATTTAAGTTCAGAAGTCGATAGCAAAAGAATTTTTATCGATAGCTATGGTAAATGCGTCTTAAACAATACTTTTCATGGGTTTAATAAATGAATTATGAATTATTTTGTGTTTGTGCTATTTAAACCTGAGAATTTGTCCATGTAGGTCTTGAGTTGCTTACTGAGCTACTTGGCTCATCCTCTAAGGAGGGATCGGAAGCTGCTGCGGCTTTATTCAAGTTGGCCAGTAAATTCTTGGCCCCATCTCCGGCTACTTCTTTATCAATACATCCGGTAAGCTTAAATCCGGTGGATTTATCATCATCCTCACAAGCTTAATATCTTACTTCCTAGAATTTAATAAAAAAATGTAAGTGTTCTGTATGATTTCAATCGGCTTCATAAGGATATAAGGCATTTTCTCGGTTCTTTTTCCATGTCTTCCATTCCAGTTGCATACGAATTTGGCATATAGCTCCCTTTCACTATGTTTGTTTATAATTATTATGGAATATGGCGTTTCTATAAGCTGCATTATCTATGCGTAAAAAAAAAACTGGATTATTTATGATCATACTATTCTTGCAGGACAAATATAGAGAGAGTTTTGCTGATACTGATGGAAAAATGTCAGACGTTATATTTCTTATTGAAGGTTGATCTCTTCTGTCTATTGTTTCATCCTTAAACTGTTTGCCTTATCTTAAAGAAAGTATTGTTGCTTCAGTTGATATGTGAAATTAACTTGGCATTATATGTTCTTTGTTGTAGGCAAACTGCTGTATGCACATAAATCTCGAATAATAAATGCTTCAAAGGTGTTGAGTGCTATGGTTACTAAGTCGGAGTCTTTTGTGAGTTTTAACCATTCAACTCTTAATTATCAACATTACTCCTCATACTTAGAATAAAACTATCAACATTACTCCTCTCATATTTGATACTTGGAATGAACTGTTAAAAATGCTTGGAAACTGCCTTTTCTTAAAGAAAGTATTGATGCTTTTTTTTTTCTTTTTTTTTTTTGTGAAAAAAGAGGGGCATAGCCCCAAACAAAAACCTATGTTATAACTTGTGGAATTACATCAACACCATCATCCTCCATAAAGGAGTTTTACAATGAAATCAGGAATTGAGACAAAAATAACATCCTGCACTACTAACTACAGGCCATACTTTGCTAATATATCTGCTACACTATTTACAGATCTAGGAACAAATGAGAAACATTCTGAACTAAAACAGCCATTTTTTTTTGTTTCTCTTAGACACCTGCACAAAACACGGGTGGGTACGGGGACAATTTCGATCTAGCGAGTGTTCTGCCACCTTGGAACCGGTCTCCAAAATCACGCTAGAACAGCTGTGATCCGTGGCACTCTTCAATCCCCAATAAATTACCCACAAGTAGCCTGAATAACTACCATTAGAGTTGCAGGCAATCTCTCCACAGTCTGCATTACTTCCGATACGCGTGAGTGCAGCGTCACAATTCACTTTGGTTAATCAGCAGCTGGTGCAGACCAGTGAAAGTCTGTTGTTAGGCTGATGTAGCCATGGTGAGATGTGATGGGAATGACTTCTTCCATAGTCTTTCGAATACCCGGAATTAACGTCTTTGTTGCCTGAGCTATAATTTGGTGATGAGAATGTGACATGTTGTTGCAGATTAATTCATTCCTACACATTCCAAATACGGTCCAGACCAGTTCAAAAGTAGTATCCCAACAAGTAGCATTTTTTCCTTTGACAAGAGACCAATAAGTTTCCCTTCATCCATTCCTCCACAGTTGTCATTCTGAAAAAGGTTAGTGAAAGCTATACCGAAGCTGCTTCAAATTTGCTTAACAAATGAGCAGTCCTTGAATGCATGAATGGTAGACTCAGCCTGTAGATTACATAAAGGACAGTATACATGTCCGAATCAGACAGGAATATTGTAACTTGTGCAATAGTATCTGCCAAAGAAATAGGAACAACTACATAATTTGTAAGAACCCCTATATTTGCGATCCATGGGTCAGTCCAAAAATTAACATTGTTACCATTTCCTACTCTCCACACAATATTTGACATCACTCTTTCCCAGCTGCTGCACACATCTTGCCAAAAATTTGATCCTGATCTCTTAATTCATTTGGATGACAGGTAGATACCACTGTACCAGCGGGCACTGCACACGTAGTGATCACTGAAGTGAAATATAAAGTTTTTGAAAGGATATTGAGGTCTGATTCTAATGTTTTACAAAATTTCTTACACATAAGCTTCTAATGTTTGCTGGCAATATGTATGATTGTTTTTTTGATATTTGTTTAGATTTATTTACACTGGAAACACATATGTCAGTGTGGATACTGCTAGAGAACTTTTTGAAGCTGCTAGTTATTTTGATTTAGCAGAACTAATGAAACTCTGTGAAAATAAGATTGCTCAGGTCAGTGAGAAAAAAAATTGGTTTTATAGTCTATGTTTACTCTTCTTTTATATCATAAATATCTTTATCTGTGTGTACATGGATGTATGTGAAATGGCCCCAAATGGGTGTCCATATAACCTGATGTATATTTTTGTCGCACAGAATGTACTTAGCGTGGAAACTATATGGCTTTTATATGAAGCTGCTGAAGCTTTCGTTGCTCTAACATTAAAGGGTCACTGTATCATGTTTGCATTGAGAAACTATGAAGAGTTGAGGGAAAGGTATTGAATTTGATTTATTTTCAATGGTGCTGTCTTTATTTTGTTTGTTTTCACGGTGACTCATGCTATTGACCCAAAATGATATTTAGATACCGTTCTAGATGATTTCTAATACCAATTGTTTGTTGTCTAATTGTGTTATCATGATACTTGTCTGTAGGAAACCTGACTTTATTGACAACATGATACCTAAAATACACCAACAGATGGTACACCTCTTACGCAGTGGCAGTTTAGATTGTGCGCGTCCAGACCCACAGTAG
Protein sequence

>MS.gene059476.t1

MINYGNRNTQSLDDDNIRSFTEFLNDVTITEYDEEYSSKIEEFKNRANFILRMAFHERIVKRMVDLGVVPALARHLVSPITEVVLITLRALKNFAKQYQEQFDETRAVSQIINFLNNSQNEAKHSLIYSSIDILTTLARTSTTMLDSIRIADGIPSLVHLLDDIVHQRVACFALEVLSTNHENATQIVGLGAVPLIILMLYREDTQLHNSVFQMIYNLDGACEIVRRGYLVEAFHPLTELMSSTYFESQRLAIDLLDLIVKTNPQWKIMLIQRGLIPLLTKALTSANEGVQESSVFLLCFLSEDSDCQVGIVNEGLVELLKVLDTKLYSLQVGAAFTLHRLADNKDNVYKFIMLGGVQRLLQGGYLGDTQAFDLFRLTLESLAEKINGHVLTQLLHRMWSLPNDFQTRIALALAYLSSEVDSKRIFIDSYGLELLTELLGSSSKEGSEAAAALFKLASKFLAPSPATSLSIHPDKYRESFADTDGKMSDVIFLIEGKLLYAHKSRIINASKVLSAMVTKSESFVDTTVPAGTAHVVITEVKYKVFERILRFIYTGNTYVSVDTARELFEAASYFDLAELMKLCENKIAQNVLSVETIWLLYEAAEAFVALTLKGHCIMFALRNYEELRERKPDFIDNMIPKIHQQMVHLLRSGSLDCARPDPQ