AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059513

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059513.t1 MTR_4g084550 90.761 184 7 3 1 182 1 176 5.06e-119 338
MS.gene059513.t1 MTR_8g107210 35.273 275 112 8 65 283 94 358 4.33e-36 132
MS.gene059513.t1 MTR_7g060630 71.429 70 20 0 60 129 1 70 1.00e-29 112
MS.gene059513.t1 MTR_5g021930 67.164 67 21 1 63 128 26 92 7.71e-26 103
MS.gene059513.t1 MTR_1g019130 68.750 64 20 0 66 129 89 152 1.10e-25 101
MS.gene059513.t1 MTR_5g081990 61.765 68 26 0 67 134 33 100 1.25e-25 100
MS.gene059513.t1 MTR_1g019130 68.750 64 20 0 66 129 89 152 2.37e-25 101
MS.gene059513.t1 MTR_8g095580 84.615 52 8 0 78 129 93 144 4.06e-25 98.6
MS.gene059513.t1 MTR_3g088485 84.615 52 8 0 78 129 91 142 4.85e-25 98.2
MS.gene059513.t1 MTR_4g063735 66.667 63 21 0 67 129 25 87 1.26e-24 99.8
MS.gene059513.t1 MTR_7g025010 46.377 69 37 0 62 130 42 110 3.01e-17 82.0
MS.gene059513.t1 MTR_1g115315 46.341 82 38 1 67 148 85 160 9.61e-17 78.2
MS.gene059513.t1 MTR_1g115315 46.341 82 38 1 67 148 85 160 9.69e-17 78.6
MS.gene059513.t1 MTR_2g015000 44.928 69 38 0 62 130 50 118 1.39e-16 79.7
MS.gene059513.t1 MTR_4g088070 44.776 67 37 0 61 127 23 89 1.69e-15 76.3
MS.gene059513.t1 MTR_4g088080 38.356 73 45 0 55 127 28 100 4.47e-13 68.6
MS.gene059513.t1 MTR_3g115620 42.623 61 35 0 67 127 82 142 1.10e-12 66.2
MS.gene059513.t1 MTR_6g027390 39.474 76 46 0 66 141 31 106 3.63e-12 65.9
MS.gene059513.t1 MTR_7g023810 32.743 113 66 1 47 159 48 150 5.96e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059513.t1 AT3G18010 55.285 123 41 4 65 182 74 187 1.02e-34 128
MS.gene059513.t1 AT2G28610 65.714 70 24 0 60 129 1 70 4.74e-28 108
MS.gene059513.t1 AT2G01500 51.485 101 42 2 27 121 16 115 1.13e-26 105
MS.gene059513.t1 AT5G59340 64.706 68 24 0 65 132 12 79 2.85e-26 104
MS.gene059513.t1 AT3G11260 44.444 108 50 1 54 151 11 118 2.74e-25 99.8
MS.gene059513.t1 AT1G46480 63.768 69 24 1 61 129 85 152 4.72e-25 99.4
MS.gene059513.t1 AT2G17950 60.759 79 30 1 63 140 34 112 1.14e-24 100
MS.gene059513.t1 AT1G46480 63.768 69 24 1 61 129 85 152 2.44e-24 99.0
MS.gene059513.t1 AT5G05770 58.462 65 27 0 66 130 28 92 7.66e-22 88.6
MS.gene059513.t1 AT2G33880 36.842 114 71 1 18 130 5 118 6.67e-17 80.1
MS.gene059513.t1 AT2G33880 36.842 114 71 1 18 130 5 118 8.00e-17 80.1
MS.gene059513.t1 AT5G45980 37.736 106 45 3 38 128 17 116 2.15e-15 75.5
MS.gene059513.t1 AT4G35550 49.180 61 31 0 67 127 99 159 1.04e-14 72.8
MS.gene059513.t1 AT1G20700 45.000 60 33 0 67 126 33 92 2.50e-13 66.6
MS.gene059513.t1 AT1G20700 45.000 60 33 0 67 126 95 154 8.00e-13 66.2
MS.gene059513.t1 AT1G20700 45.000 60 33 0 67 126 95 154 9.30e-13 66.2
MS.gene059513.t1 AT3G03660 49.057 53 27 0 66 118 32 84 4.14e-12 65.5
MS.gene059513.t1 AT3G03660 49.057 53 27 0 66 118 32 84 6.10e-12 65.1
MS.gene059513.t1 AT3G03660 49.057 53 27 0 66 118 32 84 8.67e-12 64.7
MS.gene059513.t1 AT3G03660 49.057 53 27 0 66 118 32 84 1.19e-11 64.7

Find 55 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAGGAATTTCAATATTT+TGG 0.107761 4.4:-27554545 None:intergenic
GCTCTCTCGCCTTGTGATTC+TGG 0.194023 4.4:-27553440 None:intergenic
GCTTCGCAGCTTCGACAATT+TGG 0.206061 4.4:+27553384 MS.gene059513:CDS
TGGCTACATCAATTTCTTAA+AGG 0.237847 4.4:-27554867 None:intergenic
TGGGAATTATGGTTGGAATT+TGG 0.252701 4.4:-27552453 None:intergenic
TTGAAGGTGCATGGGAATTA+TGG 0.269434 4.4:-27552464 None:intergenic
TAGCAGGATATAGCAGAAAA+AGG 0.336272 4.4:+27554474 MS.gene059513:intron
AGAGAAAGAGAAGTACGTTA+TGG 0.359866 4.4:+27553527 MS.gene059513:CDS
GTAAAAGGATTGAAGCAATA+AGG 0.375522 4.4:-27554561 None:intergenic
GTTGTAGCTGGCATAGTAAA+AGG 0.395877 4.4:-27554576 None:intergenic
CGACAATTTGGAAAGATTGA+AGG 0.396848 4.4:+27553396 MS.gene059513:CDS
GAGAAAGAGAAGTACGTTAT+GGG 0.401887 4.4:+27553528 MS.gene059513:CDS
CAATGGGTGCAAATACAATA+AGG 0.401985 4.4:-27552507 None:intergenic
TCTTGTTTATCCTCCTTAGC+TGG 0.415005 4.4:-27554747 None:intergenic
GAAGCTGCGAAGCTATTTGT+TGG 0.428703 4.4:-27553374 None:intergenic
CTTGCCTATACAATTCTTCA+AGG 0.428836 4.4:-27553323 None:intergenic
TAGTCCTTGAAGAATTGTAT+AGG 0.431151 4.4:+27553319 MS.gene059513:CDS
GTTGGACTCCACCTTGAACT+TGG 0.444756 4.4:-27553282 None:intergenic
AATACAATAAGGTTGGACTA+TGG 0.444802 4.4:-27552496 None:intergenic
TTGTGTTAATGCTTCTATGA+TGG 0.459648 4.4:+27554797 MS.gene059513:CDS
CTTGAGATGGAGGAAACAAT+TGG 0.464032 4.4:+27553480 MS.gene059513:CDS
TGCATCCTCAACCAAGTTCA+AGG 0.472729 4.4:+27553271 MS.gene059513:CDS
AACAAAATGCAGATTATCCT+AGG 0.488112 4.4:+27554709 MS.gene059513:intron
CTCCACCTTGAACTTGGTTG+AGG 0.502185 4.4:-27553276 None:intergenic
TGTTGGATTTGTTCAGCTGA+TGG 0.503667 4.4:-27553357 None:intergenic
TTGTTCACTGCAGTTTGAAG+TGG 0.506780 4.4:-27554101 None:intergenic
TCAAGGACTAGTAATTGAAC+TGG 0.509163 4.4:-27553306 None:intergenic
CTTACCGAGATGGTGATTGA+AGG 0.526602 4.4:-27552544 None:intergenic
GAAGCGTCGTCGCCTTGAGA+TGG 0.530642 4.4:+27553467 MS.gene059513:CDS
TTAAGCTTGGCTTACCGAGA+TGG 0.532231 4.4:-27552554 None:intergenic
ACTAGTAATTGAACTGGTGT+TGG 0.537149 4.4:-27553300 None:intergenic
CTTGAAGAATTGTATAGGCA+AGG 0.540070 4.4:+27553324 MS.gene059513:CDS
TCAGAAAAGCAAGAAGCTGC+AGG 0.540109 4.4:+27553555 MS.gene059513:CDS
AGGGGATGAGATGGTTGCAA+TGG 0.545416 4.4:-27552524 None:intergenic
CAATTGTTTCCTCCATCTCA+AGG 0.548253 4.4:-27553479 None:intergenic
GGGTGCAAATACAATAAGGT+TGG 0.557080 4.4:-27552503 None:intergenic
GCTAAGGAGGATAAACAAGA+TGG 0.562497 4.4:+27554750 MS.gene059513:CDS
TTACCGAGATGGTGATTGAA+GGG 0.563954 4.4:-27552543 None:intergenic
GGGGATGAGATGGTTGCAAT+GGG 0.571604 4.4:-27552523 None:intergenic
ATCCCCTTCAATCACCATCT+CGG 0.572975 4.4:+27552540 MS.gene059513:CDS
AGGTGCATGGGAATTATGGT+TGG 0.574486 4.4:-27552460 None:intergenic
GCGTCGTCGCCTTGAGATGG+AGG 0.576407 4.4:+27553470 MS.gene059513:CDS
ATAAGGTTGGACTATGGAGG+AGG 0.582667 4.4:-27552490 None:intergenic
GGTGATTGAAGGGGATGAGA+TGG 0.585438 4.4:-27552533 None:intergenic
TTGAAGAATTGTATAGGCAA+GGG 0.590766 4.4:+27553325 MS.gene059513:CDS
GCTGCTGAAGATGTTGTAGC+TGG 0.592967 4.4:-27554588 None:intergenic
ACAGCTCATGCAGCTGAGCA+AGG 0.595931 4.4:+27553234 MS.gene059513:intron
ACAATAAGGTTGGACTATGG+AGG 0.614353 4.4:-27552493 None:intergenic
AAACTGCAGTGAACAAGCAG+AGG 0.632631 4.4:+27554108 MS.gene059513:CDS
ATTATGGTTGGAATTTGGAG+TGG 0.636820 4.4:-27552448 None:intergenic
TACCGAGATGGTGATTGAAG+GGG 0.640386 4.4:-27552542 None:intergenic
AGTTAAAGAGACAAAGAAGT+GGG 0.667079 4.4:+27554075 MS.gene059513:CDS
CTATTGGTTCCAGAATCACA+AGG 0.685006 4.4:+27553431 MS.gene059513:CDS
GAGTTAAAGAGACAAAGAAG+TGG 0.689037 4.4:+27554074 MS.gene059513:CDS
ATCCTCAACCAAGTTCAAGG+TGG 0.762805 4.4:+27553274 MS.gene059513:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTTCTTTCAAATA+TGG - chr4.4:27553901-27553920 None:intergenic 10.0%
!!! TAAGTTTTTATTTTTGTTTT+AGG + chr4.4:27552660-27552679 MS.gene059513:intron 10.0%
!!! AAATTATTTTAGTGTATGTA+TGG - chr4.4:27553004-27553023 None:intergenic 15.0%
!!! AATAAGGAATTTCAATATTT+TGG - chr4.4:27554548-27554567 None:intergenic 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG + chr4.4:27553953-27553972 MS.gene059513:intron 15.0%
!!! GTAATAAATCATAATTTTGA+GGG - chr4.4:27554656-27554675 None:intergenic 15.0%
!!! TAATAAATCATAATTTTGAG+GGG - chr4.4:27554655-27554674 None:intergenic 15.0%
!!! TTGTTGTTTATTCAATTTAA+TGG + chr4.4:27553922-27553941 MS.gene059513:intron 15.0%
!! AAAAGATTTCATAATCAAAG+AGG + chr4.4:27554203-27554222 MS.gene059513:intron 20.0%
!! AAGAAAAAAAAATGACCAAA+TGG - chr4.4:27553762-27553781 None:intergenic 20.0%
!! AGAAAAAAAAATGACCAAAT+GGG - chr4.4:27553761-27553780 None:intergenic 20.0%
!! ATAGTTGAAATTTACTTTGT+TGG - chr4.4:27554682-27554701 None:intergenic 20.0%
!! TCATGAGTGAAATATAATAT+AGG - chr4.4:27553639-27553658 None:intergenic 20.0%
!! TTTGAGCTATATATAAACAA+CGG + chr4.4:27553027-27553046 MS.gene059513:intron 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG - chr4.4:27553877-27553896 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG - chr4.4:27553794-27553813 None:intergenic 20.0%
!!! CTTTGATTATGAAATCTTTT+GGG - chr4.4:27554203-27554222 None:intergenic 20.0%
!!! GGTAATAAATCATAATTTTG+AGG - chr4.4:27554657-27554676 None:intergenic 20.0%
!!! GTTAAAGTGTTAAAATTAGT+AGG - chr4.4:27552713-27552732 None:intergenic 20.0%
!!! GTTTTTTTTAACTTTCTGTT+GGG + chr4.4:27554263-27554282 MS.gene059513:intron 20.0%
!!! TCTTTGATTATGAAATCTTT+TGG - chr4.4:27554204-27554223 None:intergenic 20.0%
!!! TGTTTTTTTTAACTTTCTGT+TGG + chr4.4:27554262-27554281 MS.gene059513:intron 20.0%
!!! TTTTTTTTAACTTTCTGTTG+GGG + chr4.4:27554264-27554283 MS.gene059513:intron 20.0%
! AAATATCAATTTAGCCCATT+TGG + chr4.4:27553744-27553763 MS.gene059513:intron 25.0%
! AAGAGAAGATTATACTATGA+TGG - chr4.4:27552906-27552925 None:intergenic 25.0%
! AAGGAAAAAATGTGTTCTAT+TGG + chr4.4:27553415-27553434 MS.gene059513:CDS 25.0%
! ACAGCTAGATATTATCTTTA+GGG + chr4.4:27553581-27553600 MS.gene059513:intron 25.0%
! AGAAAATAGTTTAGCTACTT+AGG - chr4.4:27553154-27553173 None:intergenic 25.0%
! AGAGAAGATTATACTATGAT+GGG - chr4.4:27552905-27552924 None:intergenic 25.0%
! AGTAGGTTTGCATTAAAAAA+TGG - chr4.4:27552696-27552715 None:intergenic 25.0%
! AGTATTGTTAGTATCATAGT+CGG + chr4.4:27552803-27552822 MS.gene059513:intron 25.0%
! AGTTAAAAAGTGGAATGATA+AGG - chr4.4:27552851-27552870 None:intergenic 25.0%
! ATCATATGACTGTATTTATC+TGG - chr4.4:27553072-27553091 None:intergenic 25.0%
! ATTGGTAAGGAATCTATTAA+TGG + chr4.4:27552614-27552633 MS.gene059513:intron 25.0%
! CATATGAGAAAGTTAAAAAG+TGG - chr4.4:27552861-27552880 None:intergenic 25.0%
! GAAAATAGTTTAGCTACTTA+GGG - chr4.4:27553153-27553172 None:intergenic 25.0%
! TACAGCTAGATATTATCTTT+AGG + chr4.4:27553580-27553599 MS.gene059513:intron 25.0%
! TATGAGTAACTATATTCATG+TGG + chr4.4:27552932-27552951 MS.gene059513:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG + chr4.4:27553846-27553865 MS.gene059513:intron 25.0%
! TTGAAATTTACTTTGTTGGA+TGG - chr4.4:27554678-27554697 None:intergenic 25.0%
! TTTATAGAAAGAATGACAGA+GGG - chr4.4:27554396-27554415 None:intergenic 25.0%
!! AAAGATACATAGCTTTTGTA+AGG + chr4.4:27553695-27553714 MS.gene059513:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG + chr4.4:27554022-27554041 MS.gene059513:intron 25.0%
!! GTTAAAAAAAACAGCTTTAG+AGG - chr4.4:27554255-27554274 None:intergenic 25.0%
!! TGTTACATTTCAGATTTTCT+TGG + chr4.4:27554295-27554314 MS.gene059513:intron 25.0%
!! TTTTATAGAAAGAATGACAG+AGG - chr4.4:27554397-27554416 None:intergenic 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG + chr4.4:27553591-27553610 MS.gene059513:intron 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG - chr4.4:27553795-27553814 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG + chr4.4:27553792-27553811 MS.gene059513:intron 25.0%
AAAAACATAGCAGAAAGACA+AGG + chr4.4:27554516-27554535 MS.gene059513:CDS 30.0%
AACAAAATGCAGATTATCCT+AGG + chr4.4:27554709-27554728 MS.gene059513:intron 30.0%
AAGAAACTCAAAAAACTGGT+TGG - chr4.4:27554847-27554866 None:intergenic 30.0%
AAGGAAGAAACTCAAAAAAC+TGG - chr4.4:27554851-27554870 None:intergenic 30.0%
AATACAATAAGGTTGGACTA+TGG - chr4.4:27552499-27552518 None:intergenic 30.0%
ACACACACTTCAAATTGTAA+GGG - chr4.4:27553992-27554011 None:intergenic 30.0%
ACCCTTAAGAAAATTAAGCT+TGG - chr4.4:27552570-27552589 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG + chr4.4:27554075-27554094 MS.gene059513:CDS 30.0%
ATTTCTCTTGTCTTTGTTGA+TGG + chr4.4:27554137-27554156 MS.gene059513:intron 30.0%
GTAAAAGGATTGAAGCAATA+AGG - chr4.4:27554564-27554583 None:intergenic 30.0%
GTATTGTTAGTATCATAGTC+GGG + chr4.4:27552804-27552823 MS.gene059513:intron 30.0%
TAGTCCTTGAAGAATTGTAT+AGG + chr4.4:27553319-27553338 MS.gene059513:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG + chr4.4:27553325-27553344 MS.gene059513:CDS 30.0%
TTGTGTTAATGCTTCTATGA+TGG + chr4.4:27554797-27554816 MS.gene059513:CDS 30.0%
! AAGGATCAATACAAGTTTTG+AGG + chr4.4:27554493-27554512 MS.gene059513:CDS 30.0%
! AGCCAAGCTTAATTTTCTTA+AGG + chr4.4:27552565-27552584 MS.gene059513:intron 30.0%
! GCCAAGCTTAATTTTCTTAA+GGG + chr4.4:27552566-27552585 MS.gene059513:intron 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG + chr4.4:27553603-27553622 MS.gene059513:intron 30.0%
!! ATCAAAGAGGTTTTGTATGT+TGG + chr4.4:27554216-27554235 MS.gene059513:intron 30.0%
!! TGAAATCTTTTGGGTATCTT+TGG - chr4.4:27554194-27554213 None:intergenic 30.0%
AAACTCAAAAAACTGGTTGG+AGG - chr4.4:27554844-27554863 None:intergenic 35.0%
ACTAGTAATTGAACTGGTGT+TGG - chr4.4:27553303-27553322 None:intergenic 35.0%
AGTATCTGCTGTAATTTAGC+AGG + chr4.4:27554458-27554477 MS.gene059513:intron 35.0%
ATTATGGTTGGAATTTGGAG+TGG - chr4.4:27552451-27552470 None:intergenic 35.0%
ATTCCTTACCAATGCCTTTA+GGG - chr4.4:27552607-27552626 None:intergenic 35.0%
CAATGGGTGCAAATACAATA+AGG - chr4.4:27552510-27552529 None:intergenic 35.0%
CACACACACTTCAAATTGTA+AGG - chr4.4:27553993-27554012 None:intergenic 35.0%
CGACAATTTGGAAAGATTGA+AGG + chr4.4:27553396-27553415 MS.gene059513:CDS 35.0%
CTTGAAGAATTGTATAGGCA+AGG + chr4.4:27553324-27553343 MS.gene059513:CDS 35.0%
CTTGCCTATACAATTCTTCA+AGG - chr4.4:27553326-27553345 None:intergenic 35.0%
GAGTTAAAGAGACAAAGAAG+TGG + chr4.4:27554074-27554093 MS.gene059513:CDS 35.0%
TAGCAGGATATAGCAGAAAA+AGG + chr4.4:27554474-27554493 MS.gene059513:intron 35.0%
TATCATGATGTGACTACTTG+CGG + chr4.4:27553099-27553118 MS.gene059513:intron 35.0%
TGGGAATTATGGTTGGAATT+TGG - chr4.4:27552456-27552475 None:intergenic 35.0%
TGGGTATCTTTGGTTAACAA+AGG - chr4.4:27554184-27554203 None:intergenic 35.0%
! ACTTGATCTTTTTCCAGCTA+AGG + chr4.4:27554734-27554753 MS.gene059513:CDS 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG + chr4.4:27553527-27553546 MS.gene059513:CDS 35.0%
! CATAGCTTTTGTAAGGATCT+CGG + chr4.4:27553702-27553721 MS.gene059513:intron 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG + chr4.4:27553528-27553547 MS.gene059513:CDS 35.0%
! TCAAGGACTAGTAATTGAAC+TGG - chr4.4:27553309-27553328 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG + chr4.4:27554029-27554048 MS.gene059513:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG + chr4.4:27554046-27554065 MS.gene059513:intron 35.0%
AAAGAATGACAGAGGGAAAG+AGG - chr4.4:27554389-27554408 None:intergenic 40.0%
AAGAATGACAGAGGGAAAGA+GGG - chr4.4:27554388-27554407 None:intergenic 40.0%
ACAATAAGGTTGGACTATGG+AGG - chr4.4:27552496-27552515 None:intergenic 40.0%
AGAATGCTAATGAATGCCCT+TGG - chr4.4:27552743-27552762 None:intergenic 40.0%
CAATTGTTTCCTCCATCTCA+AGG - chr4.4:27553482-27553501 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG + chr4.4:27553480-27553499 MS.gene059513:CDS 40.0%
CTTGTTAACCAATGCCCTAA+AGG + chr4.4:27552590-27552609 MS.gene059513:intron 40.0%
GATTCCTTACCAATGCCTTT+AGG - chr4.4:27552608-27552627 None:intergenic 40.0%
GCTAAGGAGGATAAACAAGA+TGG + chr4.4:27554750-27554769 MS.gene059513:CDS 40.0%
GGAAAAAGATCAAGTGTCCT+AGG - chr4.4:27554729-27554748 None:intergenic 40.0%
GGGTGCAAATACAATAAGGT+TGG - chr4.4:27552506-27552525 None:intergenic 40.0%
GTTAGTATCATAGTCGGGTT+GGG + chr4.4:27552809-27552828 MS.gene059513:intron 40.0%
GTTGTAGCTGGCATAGTAAA+AGG - chr4.4:27554579-27554598 None:intergenic 40.0%
TGTTAGTATCATAGTCGGGT+TGG + chr4.4:27552808-27552827 MS.gene059513:intron 40.0%
TTACCGAGATGGTGATTGAA+GGG - chr4.4:27552546-27552565 None:intergenic 40.0%
TTGTTCACTGCAGTTTGAAG+TGG - chr4.4:27554104-27554123 None:intergenic 40.0%
! ACTATGGAGGAGGTTTTTGA+AGG - chr4.4:27552483-27552502 None:intergenic 40.0%
! TCTTGTTTATCCTCCTTAGC+TGG - chr4.4:27554750-27554769 None:intergenic 40.0%
! TGATCTTTTTCCAGCTAAGG+AGG + chr4.4:27554737-27554756 MS.gene059513:CDS 40.0%
! TGTTGGATTTGTTCAGCTGA+TGG - chr4.4:27553360-27553379 None:intergenic 40.0%
! TTGAAGGTGCATGGGAATTA+TGG - chr4.4:27552467-27552486 None:intergenic 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG + chr4.4:27554052-27554071 MS.gene059513:intron 40.0%
!! CTATTGGTTCCAGAATCACA+AGG + chr4.4:27553431-27553450 MS.gene059513:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG + chr4.4:27554049-27554068 MS.gene059513:intron 40.0%
AAACTGCAGTGAACAAGCAG+AGG + chr4.4:27554108-27554127 MS.gene059513:CDS 45.0%
ACACTTTAACCAATGCGCCA+AGG + chr4.4:27552723-27552742 MS.gene059513:intron 45.0%
AGGTGCATGGGAATTATGGT+TGG - chr4.4:27552463-27552482 None:intergenic 45.0%
ATAAGGTTGGACTATGGAGG+AGG - chr4.4:27552493-27552512 None:intergenic 45.0%
ATCCCCTTCAATCACCATCT+CGG + chr4.4:27552540-27552559 MS.gene059513:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG + chr4.4:27553274-27553293 MS.gene059513:CDS 45.0%
CACTTTAACCAATGCGCCAA+GGG + chr4.4:27552724-27552743 MS.gene059513:intron 45.0%
CTTACCGAGATGGTGATTGA+AGG - chr4.4:27552547-27552566 None:intergenic 45.0%
GAAGCTGCGAAGCTATTTGT+TGG - chr4.4:27553377-27553396 None:intergenic 45.0%
TACCGAGATGGTGATTGAAG+GGG - chr4.4:27552545-27552564 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG + chr4.4:27553555-27553574 MS.gene059513:CDS 45.0%
TGCATCCTCAACCAAGTTCA+AGG + chr4.4:27553271-27553290 MS.gene059513:CDS 45.0%
TTAAGCTTGGCTTACCGAGA+TGG - chr4.4:27552557-27552576 None:intergenic 45.0%
! AACCAATGCCCTAAAGGCAT+TGG + chr4.4:27552596-27552615 MS.gene059513:intron 45.0%
!! AGGAGGTTTTTGAAGGTGCA+TGG - chr4.4:27552476-27552495 None:intergenic 45.0%
!! ATGCCCTAAAGGCATTGGTA+AGG + chr4.4:27552601-27552620 MS.gene059513:intron 45.0%
!! GGAGGTTTTTGAAGGTGCAT+GGG - chr4.4:27552475-27552494 None:intergenic 45.0%
!! TACCAATGCCTTTAGGGCAT+TGG - chr4.4:27552601-27552620 None:intergenic 45.0%
AATGAATGCCCTTGGCGCAT+TGG - chr4.4:27552735-27552754 None:intergenic 50.0%
AGGGGATGAGATGGTTGCAA+TGG - chr4.4:27552527-27552546 None:intergenic 50.0%
CTCCACCTTGAACTTGGTTG+AGG - chr4.4:27553279-27553298 None:intergenic 50.0%
GCTGCTGAAGATGTTGTAGC+TGG - chr4.4:27554591-27554610 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG + chr4.4:27553384-27553403 MS.gene059513:CDS 50.0%
GGGGATGAGATGGTTGCAAT+GGG - chr4.4:27552526-27552545 None:intergenic 50.0%
GGTGATTGAAGGGGATGAGA+TGG - chr4.4:27552536-27552555 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG - chr4.4:27553285-27553304 None:intergenic 50.0%
ACAGCTCATGCAGCTGAGCA+AGG + chr4.4:27553234-27553253 MS.gene059513:intron 55.0%
GCTCTCTCGCCTTGTGATTC+TGG - chr4.4:27553443-27553462 None:intergenic 55.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG + chr4.4:27553467-27553486 MS.gene059513:CDS 60.0%
GCGTCGTCGCCTTGAGATGG+AGG + chr4.4:27553470-27553489 MS.gene059513:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 27552417 27554881 27552417 ID=MS.gene059513
chr4.4 mRNA 27552417 27554881 27552417 ID=MS.gene059513.t1;Parent=MS.gene059513
chr4.4 exon 27552417 27552561 27552417 ID=MS.gene059513.t1.exon1;Parent=MS.gene059513.t1
chr4.4 CDS 27552417 27552561 27552417 ID=cds.MS.gene059513.t1;Parent=MS.gene059513.t1
chr4.4 exon 27553238 27553576 27553238 ID=MS.gene059513.t1.exon2;Parent=MS.gene059513.t1
chr4.4 CDS 27553238 27553576 27553238 ID=cds.MS.gene059513.t1;Parent=MS.gene059513.t1
chr4.4 exon 27554068 27554129 27554068 ID=MS.gene059513.t1.exon3;Parent=MS.gene059513.t1
chr4.4 CDS 27554068 27554129 27554068 ID=cds.MS.gene059513.t1;Parent=MS.gene059513.t1
chr4.4 exon 27554480 27554624 27554480 ID=MS.gene059513.t1.exon4;Parent=MS.gene059513.t1
chr4.4 CDS 27554480 27554624 27554480 ID=cds.MS.gene059513.t1;Parent=MS.gene059513.t1
chr4.4 exon 27554721 27554881 27554721 ID=MS.gene059513.t1.exon5;Parent=MS.gene059513.t1
chr4.4 CDS 27554721 27554881 27554721 ID=cds.MS.gene059513.t1;Parent=MS.gene059513.t1
Gene Sequence

>MS.gene059513

ATGTCTGATTCATTCTGTAGCTTTCTCTCTGCCACTCCAAATTCCAACCATAATTCCCATGCACCTTCAAAAACCTCCTCCATAGTCCAACCTTATTGTATTTGCACCCATTGCAACCATCTCATCCCCTTCAATCACCATCTCGGTAAGCCAAGCTTAATTTTCTTAAGGGTCTTGTTAACCAATGCCCTAAAGGCATTGGTAAGGAATCTATTAATGGAAAGTTAATTTATCTTGAAAATATAAGTTTTTATTTTTGTTTTAGGAATAATTACACCATTTTTTAATGCAAACCTACTAATTTTAACACTTTAACCAATGCGCCAAGGGCATTCATTAGCATTCTACTTTTCTTAAATATCTCTCTTATTTTAACAGAAAATATTAGTATTGTTAGTATCATAGTCGGGTTGGGATCGAACATTTGACATCCTTATCATTCCACTTTTTAACTTTCTCATATGTTTGAATATTTTCTCGAATATCCCATCATAGTATAATCTTCTCTTTTTCAATATGAGTAACTATATTCATGTGGTCATTTACTTATTTACTTGTTTGTATATTTCTGCTAAGAAATTTGACCATACATACACTAAAATAATTTGATTTTGAGCTATATATAAACAACGGTTTTAAATTGCAAGAAATTCCAGATAAATACAGTCATATGATATCATAATATCATGATGTGACTACTTGCGGCAGTAATTTAAAAATCATGTTATATATGCCCTAAGTAGCTAAACTATTTTCTTCATGTGTTTTTTTTTTTTTTTTTGAACAATTTTCTTCATGTGTTTTGTATATATATTATACAGCTCATGCAGCTGAGCAAGGTACAAGCAATACTATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGGTATACAGCTAGATATTATCTTTAGGGTTATTCAAGGTTCTTTTAGTGGTCAAAAGATTCCTATATTATATTTCACTCATGACATTACAAGTTACAGCAGAGAGAGAGAGAGACAGAAAAAGATACATAGCTTTTGTAAGGATCTCGGTAAATTTAACAAATTTACAAAATATCAATTTAGCCCATTTGGTCATTTTTTTTTCTTCAAATATAGCCCTACAATTTTAGAGATTTTAGGTTTATTCTGATGCAACAAGAAACTATAGAAGTCAATATGATATTCTTGCATAGGATAAACCTAACATCTTTAAAAGTATAGTGCCATATTTGAAAGAAAAAAATTTATTGTTGTTTATTCAATTTAATGGACTAAATTATTGCTATTTTTGAAATTTTAGGCATTAAATTAACACCCTTACAATTTGAAGTGTGTGTGAATATGTATATGTATATGAGTTGAATTTGTGGTGATTGGATTTTGAAAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAGGTCTGTAATTTCTCTTGTCTTTGTTGATGGTACAATTACAACATTTATTCACCTTTGTTAACCAAAGATACCCAAAAGATTTCATAATCAAAGAGGTTTTGTATGTTGGAAAAAAAGAGTGTCCTCTAAAGCTGTTTTTTTTAACTTTCTGTTGGGGCTGTTTTATGTTACATTTCAGATTTTCTTGGACTTTTTGTGAATTCAATCTGAAAAAAAATGAATTCTCTTCAGACTTCAATCACATATTATCACTCTCCCTCTTTCCCTCTGTCATTCTTTCTATAAAAATAATTAAATAATTTAAATATTATTTTTTACTTTTAACTTTAGTATCTGCTGTAATTTAGCAGGATATAGCAGAAAAAGGATCAATACAAGTTTTGAGGAAAAACATAGCAGAAAGACAAGGAAAATGCCAAAATATTGAAATTCCTTATTGCTTCAATCCTTTTACTATGCCAGCTACAACATCTTCAGCAGCATATAGAACAACATGTAATAATAATAATACACAACTTTTAACCCCTCAAAATTATGATTTATTACCATCCAACAAAGTAAATTTCAACTATTATGAAGAACAAAATGCAGATTATCCTAGGACACTTGATCTTTTTCCAGCTAAGGAGGATAAACAAGATGGAATTTCTGAGAGAAAATCTATGTTTTGTGTTAATGCTTCTATGATGGACACTCATGAAATCAACTCTTCCTCCAACCAGTTTTTTGAGTTTCTTCCTTTAAGAAATTGA
Protein sequence

>MS.gene059513.t1

MSDSFCSFLSATPNSNHNSHAPSKTSSIVQPYCICTHCNHLIPFNHHLAHAAEQGTSNTMHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRRRLEMEETIGSSSTEDKKEKEKYVMGNSEKQEAAGGGVKETKKWATTSNCSEQAEDIAEKGSIQVLRKNIAERQGKCQNIEIPYCFNPFTMPATTSSAAYRTTYYPRTLDLFPAKEDKQDGISERKSMFCVNASMMDTHEINSSSNQFFEFLPLRN