Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059775.t1 | XP_013464662.1 | 94.1 | 68 | 4 | 0 | 5 | 72 | 104 | 171 | 2.30E-27 | 131 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059775.t1 | Q9S7A9 | 66.0 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.0e-07 | 57.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059775.t1 | A0A072VA58 | 94.1 | 68 | 4 | 0 | 5 | 72 | 104 | 171 | 1.6e-27 | 131.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene059775.t1 | TF | SBP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059775.t1 | MTR_2g078770 | 94.118 | 68 | 4 | 0 | 5 | 72 | 104 | 171 | 6.74e-42 | 134 |
| MS.gene059775.t1 | MTR_8g463140 | 55.714 | 70 | 29 | 1 | 5 | 72 | 111 | 180 | 4.03e-17 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059775.t1 | AT1G53160 | 65.957 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.07e-12 | 59.7 |
| MS.gene059775.t1 | AT1G53160 | 65.957 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.07e-12 | 59.7 |
| MS.gene059775.t1 | AT1G53160 | 65.957 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.07e-12 | 59.7 |
| MS.gene059775.t1 | AT3G15270 | 74.359 | 39 | 10 | 0 | 5 | 43 | 109 | 147 | 2.14e-12 | 58.9 |
| MS.gene059775.t1 | AT2G33810 | 86.667 | 30 | 4 | 0 | 5 | 34 | 100 | 129 | 4.47e-11 | 54.7 |
Find 9 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAACATAACAAGTAGTTC+TGG | 0.412406 | 2.4:+19691227 | MS.gene059775:CDS |
| AGCAAAATCATGATGGTGAT+TGG | 0.426111 | 2.4:+19691192 | MS.gene059775:CDS |
| TGCACTGAAGGAAGCGGTAC+TGG | 0.443322 | 2.4:+19691167 | MS.gene059775:CDS |
| CGGCGTCGTAAAAGCACTGC+TGG | 0.466987 | 2.4:+19691140 | MS.gene059775:CDS |
| ACTGCTGGAACTTGCACTGA+AGG | 0.529046 | 2.4:+19691155 | MS.gene059775:CDS |
| AACCTTTGATGAGTGTGCGC+AGG | 0.591425 | 2.4:+19691048 | None:intergenic |
| GGAACTTGCACTGAAGGAAG+CGG | 0.610870 | 2.4:+19691161 | MS.gene059775:CDS |
| ACTGGCCAGCAAAATCATGA+TGG | 0.639807 | 2.4:+19691185 | MS.gene059775:CDS |
| GCTTAGCGCGACACAATGAA+CGG | 0.657384 | 2.4:+19691120 | MS.gene059775:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGAACATAACAAGTAGTTC+TGG | + | chr2.4:19691227-19691246 | MS.gene059775:CDS | 30.0% | |
| ! | AATCACCATCATGATTTTGC+TGG | - | chr2.4:19691193-19691212 | None:intergenic | 35.0% |
| ! | AGCAAAATCATGATGGTGAT+TGG | + | chr2.4:19691192-19691211 | MS.gene059775:CDS | 35.0% |
| ACTGGCCAGCAAAATCATGA+TGG | + | chr2.4:19691185-19691204 | MS.gene059775:CDS | 45.0% | |
| ACTGCTGGAACTTGCACTGA+AGG | + | chr2.4:19691155-19691174 | MS.gene059775:CDS | 50.0% | |
| GCTTAGCGCGACACAATGAA+CGG | + | chr2.4:19691120-19691139 | MS.gene059775:CDS | 50.0% | |
| GGAACTTGCACTGAAGGAAG+CGG | + | chr2.4:19691161-19691180 | MS.gene059775:CDS | 50.0% | |
| TGCACTGAAGGAAGCGGTAC+TGG | + | chr2.4:19691167-19691186 | MS.gene059775:CDS | 55.0% | |
| !! | CGGCGTCGTAAAAGCACTGC+TGG | + | chr2.4:19691140-19691159 | MS.gene059775:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 19691056 | 19691274 | 19691056 | ID=MS.gene059775 |
| chr2.4 | mRNA | 19691056 | 19691274 | 19691056 | ID=MS.gene059775.t1;Parent=MS.gene059775 |
| chr2.4 | exon | 19691056 | 19691274 | 19691056 | ID=MS.gene059775.t1.exon1;Parent=MS.gene059775.t1 |
| chr2.4 | CDS | 19691056 | 19691274 | 19691056 | ID=cds.MS.gene059775.t1;Parent=MS.gene059775.t1 |
| Gene Sequence |
| Protein sequence |
