Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059887.t1 | RZB87595.1 | 84 | 75 | 12 | 0 | 5 | 79 | 579 | 653 | 7.80E-29 | 136 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059887.t1 | Q8W595 | 68.9 | 74 | 23 | 0 | 5 | 78 | 356 | 429 | 5.0e-24 | 111.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059887.t1 | A0A0R0I2D0 | 84.0 | 75 | 12 | 0 | 5 | 79 | 563 | 637 | 5.6e-29 | 136.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene059887.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059887.t1 | MTR_1g080340 | 82.667 | 75 | 13 | 0 | 5 | 79 | 480 | 554 | 2.09e-37 | 131 |
| MS.gene059887.t1 | MTR_1g069570 | 59.740 | 77 | 30 | 1 | 3 | 79 | 639 | 714 | 3.23e-27 | 102 |
| MS.gene059887.t1 | MTR_7g098390 | 54.667 | 75 | 33 | 1 | 5 | 79 | 597 | 670 | 7.76e-23 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059887.t1 | AT3G04380 | 68.000 | 75 | 24 | 0 | 5 | 79 | 340 | 414 | 2.52e-31 | 113 |
| MS.gene059887.t1 | AT3G04380 | 68.000 | 75 | 24 | 0 | 5 | 79 | 356 | 430 | 3.00e-31 | 113 |
| MS.gene059887.t1 | AT3G04380 | 68.000 | 75 | 24 | 0 | 5 | 79 | 329 | 403 | 3.46e-31 | 113 |
| MS.gene059887.t1 | AT3G04380 | 68.000 | 75 | 24 | 0 | 5 | 79 | 356 | 430 | 4.19e-31 | 113 |
| MS.gene059887.t1 | AT3G04380 | 68.000 | 75 | 24 | 0 | 5 | 79 | 313 | 387 | 4.66e-31 | 112 |
| MS.gene059887.t1 | AT3G04380 | 68.000 | 75 | 24 | 0 | 5 | 79 | 329 | 403 | 5.45e-31 | 112 |
| MS.gene059887.t1 | AT1G04050 | 55.405 | 74 | 33 | 0 | 6 | 79 | 618 | 691 | 3.09e-24 | 94.7 |
| MS.gene059887.t1 | AT1G04050 | 55.405 | 74 | 33 | 0 | 6 | 79 | 618 | 691 | 3.09e-24 | 94.7 |
| MS.gene059887.t1 | AT1G04050 | 55.405 | 74 | 33 | 0 | 6 | 79 | 514 | 587 | 5.17e-24 | 94.0 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 624 | 697 | 1.06e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 624 | 697 | 1.06e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 604 | 677 | 1.08e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 604 | 677 | 1.08e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 532 | 605 | 1.13e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 532 | 605 | 1.13e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 532 | 605 | 1.13e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 532 | 605 | 1.13e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 601 | 674 | 1.19e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 601 | 674 | 1.19e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 601 | 674 | 1.19e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 601 | 674 | 1.19e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 581 | 654 | 1.22e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 581 | 654 | 1.22e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 581 | 654 | 1.22e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 581 | 654 | 1.22e-20 | 84.3 |
| MS.gene059887.t1 | AT5G43990 | 55.405 | 74 | 33 | 0 | 6 | 79 | 512 | 585 | 1.29e-20 | 84.3 |
Find 15 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAGACATTCTTGTTGAAT+TGG | 0.291068 | 2.2:-15218079 | MS.gene059887:CDS |
| GGGTCTAGAAGGTGGATTAA+AGG | 0.309754 | 2.2:-15218276 | MS.gene059887:CDS |
| GGATGAAGAGGCTTTATGTT+TGG | 0.351749 | 2.2:-15218255 | MS.gene059887:CDS |
| GCATCAAGCAATACAGGATA+TGG | 0.359471 | 2.2:+15218305 | None:intergenic |
| CTGTATTGCTTGATGCAGAC+TGG | 0.365183 | 2.2:-15218298 | MS.gene059887:CDS |
| GATGCAGACTGGGGTCTAGA+AGG | 0.429515 | 2.2:-15218287 | MS.gene059887:CDS |
| CAGTCTGCATCAAGCAATAC+AGG | 0.456364 | 2.2:+15218299 | None:intergenic |
| TGTATTGCTTGATGCAGACT+GGG | 0.475329 | 2.2:-15218297 | MS.gene059887:CDS |
| TTGCAAGATTCATCCATCAT+AGG | 0.475638 | 2.2:-15218211 | MS.gene059887:intron |
| ACATGATAACAGTGATGGTC+AGG | 0.477703 | 2.2:+15218051 | None:intergenic |
| AATTTGAAAAGCACCTATGA+TGG | 0.482877 | 2.2:+15218198 | None:intergenic |
| AGGTGGATTAAAGGATGAAG+AGG | 0.543415 | 2.2:-15218267 | MS.gene059887:CDS |
| TTCTCACATGATAACAGTGA+TGG | 0.600443 | 2.2:+15218046 | None:intergenic |
| GCAGACTGGGGTCTAGAAGG+TGG | 0.617117 | 2.2:-15218284 | MS.gene059887:CDS |
| GTATTGCTTGATGCAGACTG+GGG | 0.684028 | 2.2:-15218296 | MS.gene059887:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTACATTAATTAATATGTTA+AGG | + | chr2.2:15218194-15218213 | None:intergenic | 15.0% |
| !!! | TATTATAGTTTTTTTTGACA+AGG | - | chr2.2:15218149-15218168 | MS.gene059887:intron | 15.0% |
| !!! | TGAATTTTTATAGTTTGTTT+TGG | - | chr2.2:15218253-15218272 | MS.gene059887:CDS | 15.0% |
| !! | AATTAATGTACCTAAATCAA+TGG | - | chr2.2:15218203-15218222 | MS.gene059887:intron | 20.0% |
| !! | CATATATAACATGATACAAA+AGG | + | chr2.2:15218023-15218042 | None:intergenic | 20.0% |
| ! | AATAGACATTCTTGTTGAAT+TGG | - | chr2.2:15218087-15218106 | MS.gene059887:CDS | 25.0% |
| ! | CTATAAAAATTCATCCTCAA+AGG | + | chr2.2:15218246-15218265 | None:intergenic | 25.0% |
| !!! | CACATTTTTGTTTGTAAAGA+GGG | + | chr2.2:15218296-15218315 | None:intergenic | 25.0% |
| !!! | TATAGTTTTTTTTGACAAGG+TGG | - | chr2.2:15218152-15218171 | MS.gene059887:intron | 25.0% |
| !!! | TCACATTTTTGTTTGTAAAG+AGG | + | chr2.2:15218297-15218316 | None:intergenic | 25.0% |
| ACAATGAAAGTTAACCTTTG+AGG | - | chr2.2:15218229-15218248 | MS.gene059887:CDS | 30.0% | |
| ACTGTTATCATGTGAGAATT+TGG | - | chr2.2:15218125-15218144 | MS.gene059887:intron | 30.0% | |
| TAATGTACCTAAATCAATGG+AGG | - | chr2.2:15218206-15218225 | MS.gene059887:intron | 30.0% | |
| TTGACAAGGTGGTTTATTAT+AGG | - | chr2.2:15218163-15218182 | MS.gene059887:intron | 30.0% | |
| !! | AATTTGAAAAGCACCTATGA+TGG | + | chr2.2:15217971-15217990 | None:intergenic | 30.0% |
| TTCTCACATGATAACAGTGA+TGG | + | chr2.2:15218123-15218142 | None:intergenic | 35.0% | |
| TTGCAAGATTCATCCATCAT+AGG | - | chr2.2:15217955-15217974 | MS.gene059887:intron | 35.0% | |
| !! | TCATTGTCCTCCATTGATTT+AGG | + | chr2.2:15218216-15218235 | None:intergenic | 35.0% |
| ACATGATAACAGTGATGGTC+AGG | + | chr2.2:15218118-15218137 | None:intergenic | 40.0% | |
| CAAAAATGTGAGTGCATACG+AGG | - | chr2.2:15218306-15218325 | MS.gene059887:CDS | 40.0% | |
| GCATCAAGCAATACAGGATA+TGG | + | chr2.2:15217864-15217883 | None:intergenic | 40.0% | |
| TGTATTGCTTGATGCAGACT+GGG | - | chr2.2:15217869-15217888 | MS.gene059887:CDS | 40.0% | |
| ! | GGATGAAGAGGCTTTATGTT+TGG | - | chr2.2:15217911-15217930 | MS.gene059887:intron | 40.0% |
| ! | TAGATTTGCATCAGAGCATC+TGG | + | chr2.2:15218068-15218087 | None:intergenic | 40.0% |
| !! | AGGTGGATTAAAGGATGAAG+AGG | - | chr2.2:15217899-15217918 | MS.gene059887:intron | 40.0% |
| CAGTCTGCATCAAGCAATAC+AGG | + | chr2.2:15217870-15217889 | None:intergenic | 45.0% | |
| CTGTATTGCTTGATGCAGAC+TGG | - | chr2.2:15217868-15217887 | MS.gene059887:CDS | 45.0% | |
| GTATTGCTTGATGCAGACTG+GGG | - | chr2.2:15217870-15217889 | MS.gene059887:CDS | 45.0% | |
| !! | GGGTCTAGAAGGTGGATTAA+AGG | - | chr2.2:15217890-15217909 | MS.gene059887:intron | 45.0% |
| AAGAGGGCAAGCTGCAGACA+AGG | + | chr2.2:15218280-15218299 | None:intergenic | 55.0% | |
| GATGCAGACTGGGGTCTAGA+AGG | - | chr2.2:15217879-15217898 | MS.gene059887:CDS | 55.0% | |
| GCAGACTGGGGTCTAGAAGG+TGG | - | chr2.2:15217882-15217901 | MS.gene059887:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 15217852 | 15218336 | 15217852 | ID=MS.gene059887 |
| chr2.2 | mRNA | 15217852 | 15218336 | 15217852 | ID=MS.gene059887.t1;Parent=MS.gene059887 |
| chr2.2 | exon | 15218212 | 15218336 | 15218212 | ID=MS.gene059887.t1.exon1;Parent=MS.gene059887.t1 |
| chr2.2 | CDS | 15218212 | 15218336 | 15218212 | ID=cds.MS.gene059887.t1;Parent=MS.gene059887.t1 |
| chr2.2 | exon | 15218053 | 15218119 | 15218053 | ID=MS.gene059887.t1.exon2;Parent=MS.gene059887.t1 |
| chr2.2 | CDS | 15218053 | 15218119 | 15218053 | ID=cds.MS.gene059887.t1;Parent=MS.gene059887.t1 |
| chr2.2 | exon | 15217852 | 15217899 | 15217852 | ID=MS.gene059887.t1.exon3;Parent=MS.gene059887.t1 |
| chr2.2 | CDS | 15217852 | 15217899 | 15217852 | ID=cds.MS.gene059887.t1;Parent=MS.gene059887.t1 |
| Gene Sequence |
| Protein sequence |
